Rroxscaffold_2G00086430

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013702
Physical Location & Seq
Forward (+)
8620625 .. 8630386
9762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00086430.1

Sequence Viewer

Length: 1035 bp
ATGGGAATCAAACTATCAATTTCGTCACTTATTTTTCTGTTCTTGAGTTTTGCAAATTTTGGCTCAGTTTTAATTGATGGATCGCCGATTGCACCAGCATTATACGTGTTTGGGGATTCTTTGTTTGATAGTGGTAACAATAATTTCTTGCCAACTTTAGCAAAGGCAGATTTCCTGCCCTATGGTGTCAATTTTGCGAAGGGTGTCACCGGAAGATTTACCAACGGTAAAACAGTAGCGGATTTTATAGGTAAAACAGGGAAGTGCTTGAATCTGAAAGAGCAGATGGATTTGTTTGAAAAAACGGTGAGGTCAGACTTGTCACGACATTTCAAAAATCCCAATGATCTTGCGGAGTACTTGTCCAAGTCCATATTTATAGTTTCTGTAGGCAACAATGATTTTCTTAATAATTACCTTCAACCTAAACTCTATAACACAAGCAAACGCTACCCTCCTCCACAGTTTGCACAACTCTTGATGGATGATCTTTCTCACCATTTTGAGAGATTATATAACTTAGGAGCCAGGAAAATAGTTATGTTTGAAATTGGTCCCCTTGGTTGCATCCCATCAATTGCAAAGACACAAAACCATAGTGGAAACTGTGCGGAAGGAACAAACAAGCTGGCCTCGATTTTTAACGACAAACTTCGTGCAACTCTCGCAAATTTAACTTTCACTTTTCAAGGATCTTTATTTGTTCTCGGTCGAGCTAACGGGATCGGCTATGATGCAATTACAAGTCCCCTCAAATATGGCCTCGAGGACGGAAGCAATCCATGCTGCACAACTTGGAACAATGGGACATCGGCATGTATTCCATGGGCTAAACCATGCTTTCAACCAAATAGTCACTTCTTCTGGGATGCTTTTCATCTTACTGAATCTGCTAGTTCAGTCGTAGCAACTGGCTGTTTCAATGATACAACTGTTTGCACTCCACTAAACATCAAGAAACTTGTACAAGTGGAATCGAAGTTGAGAGAAAATCGCTGCTGTGTTCTCATTGATAATAGGGGCCTCTTTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

344

Amino Acids

37.96

Weight (kDa)

8.57

Isoelectric Point (pI)

18.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 303 4.3e-22 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 764
AciI CCGC 3 cut(s) 239, 353, 611
AclWI GGATC 3 cut(s) 88, 700, 731
AcsI RAATTY 2 cut(s) 55, 670
AfaI GTAC 2 cut(s) 359, 966
AflIII ACRYGT 1 cut(s) 105
AgsI TTSAA 8 cut(s) 271, 299, 334, 422, 548, 689, 845, 922
AjnI CCWGG 1 cut(s) 527
AluBI AGCT 2 cut(s) 628, 716
AluI AGCT 2 cut(s) 628, 716
AlwI GGATC 3 cut(s) 88, 700, 731
Ama87I CYCGRG 1 cut(s) 764
AoxI GGCC 3 cut(s) 630, 760, 1021
ApeKI GCWGC 2 cut(s) 786, 996
ApoI RAATTY 2 cut(s) 55, 670
AspS9I GGNCC 2 cut(s) 554, 1021
AsuHPI GGTGA 3 cut(s) 199, 319, 488
AvaI CYCGRG 1 cut(s) 764
AvaII GGWCC 1 cut(s) 554
BaeI ACNNNNGTAYC 2 cut(s) 918, 951
BbvI GCAGC 2 cut(s) 773, 983
BccI CCATC 4 cut(s) 71, 280, 475, 580
BciT130I CCWGG 1 cut(s) 529
BfaI CTAG 1 cut(s) 894
BfmI CTRYAG 1 cut(s) 387
BisI GCNGC 2 cut(s) 787, 997
BlsI GCNGC 2 cut(s) 788, 998
BmcAI AGTACT 1 cut(s) 359
Bme1390I CCNGG 1 cut(s) 529
Bme18I GGWCC 1 cut(s) 554
BmeT110I CYCGRG 1 cut(s) 764
BmgT120I GGNCC 2 cut(s) 554, 1021
BmiI GGNNCC 3 cut(s) 526, 556, 1022
BmrFI CCNGG 1 cut(s) 529
BmsI GCATC 3 cut(s) 576, 724, 859
BpuEI CTTGAG 1 cut(s) 64
BsaAI YACGTR 1 cut(s) 106
BsaJI CCNNGG 2 cut(s) 559, 824
BsaWI WCCGGW 1 cut(s) 209
BsaXI ACNNNNNCTCC 2 cut(s) 347, 377
Bse1I ACTGG 1 cut(s) 916
BseBI CCWGG 1 cut(s) 529
BseDI CCNNGG 2 cut(s) 559, 824
BseGI GGATG 3 cut(s) 490, 567, 874
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 1 cut(s) 916
BseRI GAGGAG 1 cut(s) 447
BseXI GCAGC 2 cut(s) 773, 983
BsgI GTGCAG 1 cut(s) 772
Bsh1285I CGRYCG 1 cut(s) 712
BshFI GGCC 3 cut(s) 632, 762, 1023
BsiEI CGRYCG 1 cut(s) 712
BsiHKCI CYCGRG 1 cut(s) 764
BsiSI CCGG 1 cut(s) 210
BslFI GGGAC 3 cut(s) 540, 732, 820
BsmFI GGGAC 3 cut(s) 540, 732, 820
BsnI GGCC 3 cut(s) 632, 762, 1023
BsoBI CYCGRG 1 cut(s) 764
Bsp1407I TGTACA 1 cut(s) 964
Bsp143I GATC 5 cut(s) 80, 346, 487, 692, 723
Bsp19I CCATGG 1 cut(s) 824
BspACI CCGC 3 cut(s) 239, 353, 611
BspANI GGCC 3 cut(s) 632, 762, 1023
BspCNI CTCAG 1 cut(s) 77
BspLI GGNNCC 3 cut(s) 526, 556, 1022
BspPI GGATC 3 cut(s) 88, 700, 731
BsrGI TGTACA 1 cut(s) 964
BsrI ACTGG 1 cut(s) 916
BssECI CCNNGG 2 cut(s) 559, 824
BssMI GATC 5 cut(s) 80, 346, 487, 692, 723
BssT1I CCWWGG 2 cut(s) 559, 824
Bst2UI CCWGG 1 cut(s) 529
Bst4CI ACNGT 6 cut(s) 227, 235, 307, 465, 608, 934
BstAPI GCANNNNNTGC 1 cut(s) 783
BstAUI TGTACA 1 cut(s) 964
BstBAI YACGTR 1 cut(s) 106
BstC8I GCNNGC 1 cut(s) 630
BstDEI CTNAG 2 cut(s) 64, 520
BstDSI CCRYGG 1 cut(s) 824
BstF5I GGATG 3 cut(s) 490, 567, 874
BstKTI GATC 5 cut(s) 83, 349, 490, 695, 726
BstMBI GATC 5 cut(s) 80, 346, 487, 692, 723
BstMCI CGRYCG 1 cut(s) 712
BstMWI GCNNNNNNNGC 2 cut(s) 665, 783
BstNI CCWGG 1 cut(s) 529
BstNSI RCATGY 1 cut(s) 819
BstSCI CCNGG 1 cut(s) 527
BstSFI CTRYAG 1 cut(s) 387
BstV1I GCAGC 2 cut(s) 773, 983
BstX2I RGATCY 1 cut(s) 692
BstYI RGATCY 1 cut(s) 692
BsuRI GGCC 3 cut(s) 632, 762, 1023
BtgI CCRYGG 1 cut(s) 824
BtsCI GGATG 3 cut(s) 490, 567, 874
Cac8I GCNNGC 1 cut(s) 630
Cfr13I GGNCC 2 cut(s) 554, 1021
Csp6I GTAC 2 cut(s) 358, 965
CviAII CATG 4 cut(s) 783, 816, 825, 837
CviQI GTAC 2 cut(s) 358, 965
DdeI CTNAG 2 cut(s) 64, 520
DpnI GATC 5 cut(s) 82, 348, 489, 694, 725
DpnII GATC 5 cut(s) 80, 346, 487, 692, 723
Eco130I CCWWGG 2 cut(s) 559, 824
Eco47I GGWCC 1 cut(s) 554
Eco88I CYCGRG 1 cut(s) 764
EcoO109I RGGNCCY 1 cut(s) 1021
EcoRII CCWGG 1 cut(s) 527
EcoT14I CCWWGG 2 cut(s) 559, 824
ErhI CCWWGG 2 cut(s) 559, 824
FaeI CATG 4 cut(s) 786, 819, 828, 840
FaqI GGGAC 3 cut(s) 540, 732, 820
FatI CATG 4 cut(s) 782, 815, 824, 836
Fnu4HI GCNGC 2 cut(s) 787, 997
FokI GGATG 3 cut(s) 497, 554, 881
Fsp4HI GCNGC 2 cut(s) 787, 997
FspBI CTAG 1 cut(s) 894
GluI GCNGC 2 cut(s) 787, 997
HaeIII GGCC 3 cut(s) 632, 762, 1023
HapII CCGG 1 cut(s) 210
Hin1II CATG 4 cut(s) 786, 819, 828, 840
HinfI GANTC 5 cut(s) 6, 116, 271, 887, 974
HpaII CCGG 1 cut(s) 210
HphI GGTGA 3 cut(s) 199, 319, 488
Hpy188I TCNGA 2 cut(s) 276, 316
Hpy188III TCNNGA 4 cut(s) 43, 324, 478, 955
HpyAV CCTTC 3 cut(s) 193, 428, 608
HpyCH4III ACNGT 6 cut(s) 227, 235, 307, 465, 608, 934
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 9 cut(s) 53, 92, 470, 567, 581, 659, 737, 789, 939
HpyF10VI GCNNNNNNNGC 2 cut(s) 665, 783
HpyF3I CTNAG 2 cut(s) 64, 520
HpySE526I ACGT 1 cut(s) 105
Hsp92II CATG 4 cut(s) 786, 819, 828, 840
Kzo9I GATC 5 cut(s) 80, 346, 487, 692, 723
LmnI GCTCC 1 cut(s) 524
LpnPI CCDG 9 cut(s) 108, 188, 223, 243, 514, 541, 614, 850, 897
Lsp1109I GCAGC 2 cut(s) 773, 983
LweI GCATC 3 cut(s) 576, 724, 859
MaeI CTAG 1 cut(s) 894
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 5 cut(s) 24, 134, 205, 321, 854
MalI GATC 5 cut(s) 82, 348, 489, 694, 725
MboI GATC 5 cut(s) 80, 346, 487, 692, 723
MboII GAAGA 2 cut(s) 225, 853
MfeI CAATTG 1 cut(s) 576
MflI RGATCY 1 cut(s) 692
MnlI CCTC 8 cut(s) 303, 465, 468, 643, 760, 761, 773, 1034
MseI TTAA 4 cut(s) 71, 408, 642, 674
MslI CAYNNNNRTG 1 cut(s) 814
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 1 cut(s) 529
MunI CAATTG 1 cut(s) 576
MvaI CCWGG 1 cut(s) 529
MwoI GCNNNNNNNGC 2 cut(s) 665, 783
NcoI CCATGG 1 cut(s) 824
NdeII GATC 5 cut(s) 80, 346, 487, 692, 723
NlaIII CATG 4 cut(s) 786, 819, 828, 840
NlaIV GGNNCC 3 cut(s) 526, 556, 1022
NmuCI GTSAC 4 cut(s) 24, 205, 321, 854
NspI RCATGY 1 cut(s) 819
PaeR7I CTCGAG 1 cut(s) 764
PfeI GAWTC 5 cut(s) 6, 116, 271, 887, 974
PkrI GCNGC 2 cut(s) 788, 998
Ppu21I YACGTR 1 cut(s) 106
Psp6I CCWGG 1 cut(s) 527
PspGI CCWGG 1 cut(s) 527
PspN4I GGNNCC 3 cut(s) 526, 556, 1022
PspPI GGNCC 2 cut(s) 554, 1021
PspXI VCTCGAGB 1 cut(s) 764
PsuI RGATCY 1 cut(s) 692
RsaI GTAC 2 cut(s) 359, 966
RsaNI GTAC 2 cut(s) 358, 965
RseI CAYNNNNRTG 1 cut(s) 814
SaqAI TTAA 4 cut(s) 71, 408, 642, 674
SatI GCNGC 2 cut(s) 787, 997
Sau3AI GATC 5 cut(s) 80, 346, 487, 692, 723
Sau96I GGNCC 2 cut(s) 554, 1021
ScaI AGTACT 1 cut(s) 359
ScrFI CCNGG 1 cut(s) 529
SetI ASST 7 cut(s) 108, 253, 314, 420, 427, 630, 718
SfaNI GCATC 3 cut(s) 576, 724, 859
SfcI CTRYAG 1 cut(s) 387
Sfr274I CTCGAG 1 cut(s) 764
SinI GGWCC 1 cut(s) 554
SlaI CTCGAG 1 cut(s) 764
SmiMI CAYNNNNRTG 1 cut(s) 814
SmlI CTYRAG 2 cut(s) 43, 764
SmoI CTYRAG 2 cut(s) 43, 764
SsiI CCGC 3 cut(s) 239, 353, 611
SspMI CTAG 1 cut(s) 894
StyD4I CCNGG 1 cut(s) 527
StyI CCWWGG 2 cut(s) 559, 824
TaaI ACNGT 6 cut(s) 227, 235, 307, 465, 608, 934
TaiI ACGT 1 cut(s) 108
TaqI TCGA 4 cut(s) 635, 712, 765, 977
TaqII GACCGA 1 cut(s) 698
TatI WGTACW 2 cut(s) 357, 964
TfiI GAWTC 5 cut(s) 6, 116, 271, 887, 974
Tru1I TTAA 4 cut(s) 71, 408, 642, 674
Tru9I TTAA 4 cut(s) 71, 408, 642, 674
TseFI GTSAC 4 cut(s) 24, 205, 321, 854
TseI GCWGC 2 cut(s) 786, 996
Tsp45I GTSAC 4 cut(s) 24, 205, 321, 854
TspDTI ATGAA 1 cut(s) 866
TspGWI ACGGA 1 cut(s) 786
VpaK11BI GGWCC 1 cut(s) 554
XapI RAATTY 2 cut(s) 55, 670
XceI RCATGY 1 cut(s) 819
XhoI CTCGAG 1 cut(s) 764
XspI CTAG 1 cut(s) 894
ZrmI AGTACT 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.