FvH4_6g52400

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001269
Physical Location & Seq
Reverse (-)
38600882 .. 38605354
4473 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g52400.t2

Sequence Viewer

Length: 2082 bp
ATGGGAGACTCGACCTGTCTCATGCAACCCTTTTCTTACGCTTCTGGAATTCCCAATGAGGCATTCGAGGGGAATCCAATTCATGCTCTTGGGCATTCGGTTTCGTTTGGGAGGTTTATGACAGATTCATTAGCTTGGGAGAAATGGTCATCATTCTCTCATAATCGATATGTAGAAGAGGCTGAGAGGTATGCTCAGCCCGGCTCAGTTGCACAGAAGAAGGCTTTTTTTGAGGCACATTACAAGAAGGTTGCTGCTCAACGAGCCGCGGCTTTGCTTGAACAAGCAAATGCTGCGGCTGCGAAATCCAACAATGCTACTCCACCAAAACCCCAAGTCATTGCCCCAGATCCACCAAAGTACATGATCTGTGAGCAGGAGCTAAGGGAGAAGGTGAAACAGCGAAATGGACTCCGTTCGAAAGTTGGATTGGACAAATTCGAAAGCATTGAGATGGTGAAACTAGCTGATCTTGTCGCTGAAGAGGAGGCTGAAGACAAACATAGAGAAGAAGCTGTATTGGTGGATACCAGTGGGTATAACTCAAGCATTGACCCACAAAGAGAAGAAGGAGCTGTATTTGTGGATACCAGTGGGTATAACTCGAGCATTGATATGGAGAACTGTGAAAAAGTGCATCCTATCTCTGAATTCCCAGTTCCAGGGGAGAACTCTGATGTAGCTGAATTCTCAACCCCGATGCAAGAAACTTATACCCACAATGTGGAAACTGTCATGGAGCTCGAGCACAGTGGCACACTTCAGATGGAAAAACCTCTTTTACAGGAGAGTCGCAGCTCTGATCAAGAAGCTTCATTGGAACAAAACAAAACTCTCTTTTCATCCAAGTCATCATTAATGTATCACAAAGCACCTAAGGCACCAATTTCTCCATCCAAAACCACAGCTCCTTGTTTTCCAAGAAGGGAAGGCAATGCCACTCCATTACACAAAGCTTGTAAGGCCCCAACATTCCCATCCAAACTCACAACTCCTTGTTTTCCGAGGAGGGAAAGTAATGCCACTCCATTACTGAAAGCACCCAAAGCCCCAGCATCTCCATCCAAACCCACACCTTCTTGTTTTCCAGGAAGGGAAGGTGCCATTCCATTACACAAAGAACGTAAGGGCCCAACATCGCCAGCCAAATCCACAGCTTCTTGTTTTCCGAGAAGGGAAGGTAATGCCATTCCATCACACAAAGCACGTAATGGCCCAGCATCTCCAGCCAAACCCACAGCTTCTTGTTTTCCGAGAAGGGAAGGTAATGCCACTCCATTACATAAAATGCTGTCATCAGCTTCGGAGGACAAGAAATCTTCTACACCAAAATCGCTTCAAAAGTCTGTCAGTTTTACACCCATCAGAGAGCTCTCTAGATTTACTTCAACAGTCATGAGAAAGATTGAGAACTCAAGAGTTGGTACTAGTTCTTTCAAGACGCCTAAGGATTGCTCAACTCTTCTAAAGACTCCAACCATGGTATCTAAAAATGAGGTGCACAATCATCCTTCAGTAACTCCTTGTTCAGGAAAGAGAAGGGCAAAAACGCCAGGCGATCCCTCATCAGTCACTGGAGCGAAAACCTTGGGGCCCAAATGGCACACAATCCGTACAGATTGCTCAAAAATTTTGAGTGCCTGCAGAAACAAAGCACGATCTCCATTTTCATCTGCCTCCTTCAGCTTAAGAACTGAAGAACGAGCTGCAAGTAGAAAGAAGAAGCTCGAAGAAAAGTTCAATGCTGATGAGGCACAACAAGTGCAGGAGAAAGCAGAAATAGAAATTAGAAAGTTCCGTCAAAGCCTTTGCTTCAAAGCCAGGCCACTGCCTGATTTTTATAAAGAAAGAAAAGCACCACACAAGGATGAGGTTCTGAATACTAATTCACAGACGGTAGCGAAAAAGCATAGTCCACGAGGCGCAATCTCTGTACCTCCTCATGATCCTTCATTCAAGAGTAGTGGCTCCAAGCATGTGCAGATCCAAGCAAGGAATAAGGAAACCCCAACTTGCTCACTCATTACACGATCCTTAAAGACCGTCCATGAGAACACATCTCCAAATATTCAGTCCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

694

Amino Acids

76.34

Weight (kDa)

9.44

Isoelectric Point (pI)

55.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 552 - 613 3.5e-11 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1842
AccB1I GGYRCC 2 cut(s) 880, 1100
AccB7I CCANNNNNTGG 1 cut(s) 724
AccII CGCG 1 cut(s) 269
AciI CCGC 3 cut(s) 267, 269, 296
AclWI GGATC 5 cut(s) 344, 1553, 1940, 1978, 2025
AcsI RAATTY 5 cut(s) 48, 437, 650, 686, 1629
AcuI CTGAAG 6 cut(s) 501, 513, 746, 1497, 1666, 1716
AcyI GRCGYC 1 cut(s) 1442
AdeI CACNNNGTG 1 cut(s) 724
AfaI GTAC 4 cut(s) 362, 1426, 1615, 1935
AfiI CCNNNNNNNGG 3 cut(s) 662, 724, 1529
AflII CTTAAG 1 cut(s) 1687
AgsI TTSAA 7 cut(s) 281, 1340, 1389, 1438, 1741, 1816, 1957
AhlI ACTAGT 1 cut(s) 1427
AjnI CCWGG 4 cut(s) 661, 1087, 1552, 1820
AjuI GAANNNNNNNTTGG 4 cut(s) 47, 79, 413, 445
Alw21I GWGCWC 4 cut(s) 744, 750, 1374, 1503
Alw26I GTCTC 1 cut(s) 23
Alw44I GTGCAC 1 cut(s) 1499
AlwI GGATC 5 cut(s) 344, 1553, 1940, 1978, 2025
AlwNI CAGNNNCTG 1 cut(s) 1574
Ama87I CYCGRG 2 cut(s) 604, 743
AoxI GGCC 5 cut(s) 963, 1129, 1213, 1592, 1823
ApaI GGGCCC 2 cut(s) 1133, 1596
ApaLI GTGCAC 1 cut(s) 1499
ApeKI GCWGC 5 cut(s) 254, 293, 299, 795, 1706
ApoI RAATTY 5 cut(s) 48, 437, 650, 686, 1629
ArsI GACNNNNNNTTYG 1 cut(s) 2057
AseI ATTAAT 1 cut(s) 857
AspLEI GCGC 1 cut(s) 1925
AspS9I GGNCC 6 cut(s) 964, 1129, 1130, 1214, 1592, 1593
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 2 cut(s) 406, 469
AsuII TTCGAA 2 cut(s) 419, 441
AvaI CYCGRG 2 cut(s) 604, 743
AxyI CCTNAGG 2 cut(s) 876, 1446
BaeGI GKGCMC 3 cut(s) 1133, 1503, 1596
BaeI ACNNNNGTAYC 2 cut(s) 1467, 1500
BanI GGYRCC 2 cut(s) 880, 1100
BanII GRGCYC 4 cut(s) 744, 1133, 1374, 1596
BauI CACGAG 1 cut(s) 1917
BbsI GAAGAC 1 cut(s) 501
Bbv12I GWGCWC 4 cut(s) 744, 750, 1374, 1503
BbvI GCAGC 5 cut(s) 241, 280, 286, 807, 1693
BccI CCATC 7 cut(s) 448, 760, 901, 985, 1069, 1201, 1370
BciT130I CCWGG 4 cut(s) 663, 1089, 1554, 1822
BciVI GTATCC 2 cut(s) 520, 580
BclI TGATCA 1 cut(s) 802
BcnI CCSGG 1 cut(s) 201
BcoDI GTCTC 1 cut(s) 23
BcuI ACTAGT 1 cut(s) 1427
BfaI CTAG 3 cut(s) 464, 1377, 1428
BfmI CTRYAG 1 cut(s) 1642
BfrI CTTAAG 1 cut(s) 1687
BfuI GTATCC 2 cut(s) 520, 580
BglI GCCNNNNNGGC 1 cut(s) 1600
BisI GCNGC 8 cut(s) 255, 267, 270, 294, 297, 300, 796, 1707
BlpI GCTNAGC 1 cut(s) 195
BlsI GCNGC 8 cut(s) 256, 268, 271, 295, 298, 301, 797, 1708
Bme1390I CCNGG 5 cut(s) 201, 663, 1089, 1554, 1822
BmeT110I CYCGRG 2 cut(s) 604, 743
BmgT120I GGNCC 6 cut(s) 964, 1129, 1130, 1214, 1592, 1593
BmiI GGNNCC 7 cut(s) 882, 966, 1102, 1131, 1593, 1594, 1969
BmrFI CCNGG 5 cut(s) 201, 663, 1089, 1554, 1822
BmrI ACTGGG 1 cut(s) 650
BmsI GCATC 4 cut(s) 646, 690, 1064, 1229
BmuI ACTGGG 1 cut(s) 650
BpiI GAAGAC 1 cut(s) 501
BplI GAGNNNNNCTC 2 cut(s) 178, 210
BpmI CTGGAG 2 cut(s) 1209, 1596
Bpu10I CCTNAGC 1 cut(s) 383
Bpu1102I GCTNAGC 1 cut(s) 195
Bpu14I TTCGAA 2 cut(s) 419, 441
BpuEI CTTGAG 2 cut(s) 529, 1399
BpuMI CCSGG 1 cut(s) 201
Bsa29I ATCGAT 1 cut(s) 166
BsaAI YACGTR 1 cut(s) 1208
BsaHI GRCGYC 1 cut(s) 1442
BsaJI CCNNGG 5 cut(s) 267, 662, 1004, 1479, 1587
BsaXI ACNNNNNCTCC 6 cut(s) 131, 161, 892, 922, 1000, 1030
Bsc4I CCNNNNNNNGG 3 cut(s) 662, 724, 1529
Bse1I ACTGG 4 cut(s) 531, 591, 656, 1579
Bse21I CCTNAGG 2 cut(s) 876, 1446
Bse3DI GCAATG 2 cut(s) 339, 940
BseBI CCWGG 4 cut(s) 663, 1089, 1554, 1822
BseCI ATCGAT 1 cut(s) 166
BseDI CCNNGG 5 cut(s) 267, 662, 1004, 1479, 1587
BseGI GGATG 7 cut(s) 637, 842, 893, 977, 1061, 1507, 1873
BseLI CCNNNNNNNGG 3 cut(s) 662, 724, 1529
BseMI GCAATG 2 cut(s) 339, 940
BseMII CTCAG 3 cut(s) 174, 209, 219
BseNI ACTGG 4 cut(s) 531, 591, 656, 1579
BseRI GAGGAG 3 cut(s) 500, 1021, 1929
BseSI GKGCMC 3 cut(s) 1133, 1503, 1596
BseXI GCAGC 5 cut(s) 241, 280, 286, 807, 1693
BseYI CCCAGC 2 cut(s) 1051, 1216
BsgI GTGCAG 2 cut(s) 1784, 2000
Bsh1236I CGCG 1 cut(s) 269
BshFI GGCC 5 cut(s) 965, 1131, 1215, 1594, 1825
BshNI GGYRCC 2 cut(s) 880, 1100
BshVI ATCGAT 1 cut(s) 166
BsiHKAI GWGCWC 4 cut(s) 744, 750, 1374, 1503
BsiHKCI CYCGRG 2 cut(s) 604, 743
BsiSI CCGG 1 cut(s) 201
BslI CCNNNNNNNGG 3 cut(s) 662, 724, 1529
BsmAI GTCTC 1 cut(s) 23
BsmI GAATGC 2 cut(s) 62, 94
BsnI GGCC 5 cut(s) 965, 1131, 1215, 1594, 1825
BsoBI CYCGRG 2 cut(s) 604, 743
Bsp119I TTCGAA 2 cut(s) 419, 441
Bsp120I GGGCCC 2 cut(s) 1129, 1592
Bsp1286I GDGCHC 6 cut(s) 744, 750, 1133, 1374, 1503, 1596
Bsp143I GATC 9 cut(s) 349, 366, 469, 802, 1558, 1658, 1945, 1983, 2030
Bsp1720I GCTNAGC 1 cut(s) 195
Bsp19I CCATGG 1 cut(s) 1479
BspACI CCGC 3 cut(s) 267, 269, 296
BspANI GGCC 5 cut(s) 965, 1131, 1215, 1594, 1825
BspCNI CTCAG 3 cut(s) 175, 208, 218
BspDI ATCGAT 1 cut(s) 166
BspFNI CGCG 1 cut(s) 269
BspHI TCATGA 2 cut(s) 1395, 1942
BspLI GGNNCC 7 cut(s) 882, 966, 1102, 1131, 1593, 1594, 1969
BspMAI CTGCAG 1 cut(s) 1646
BspPI GGATC 5 cut(s) 344, 1553, 1940, 1978, 2025
BspT104I TTCGAA 2 cut(s) 419, 441
BspT107I GGYRCC 2 cut(s) 880, 1100
BspTI CTTAAG 1 cut(s) 1687
BsrDI GCAATG 2 cut(s) 339, 940
BsrI ACTGG 4 cut(s) 531, 591, 656, 1579
BssECI CCNNGG 5 cut(s) 267, 662, 1004, 1479, 1587
BssMI GATC 9 cut(s) 349, 366, 469, 802, 1558, 1658, 1945, 1983, 2030
BssNI GRCGYC 1 cut(s) 1442
BssSI CACGAG 1 cut(s) 1917
BssT1I CCWWGG 2 cut(s) 1479, 1587
Bst2BI CACGAG 1 cut(s) 1917
Bst2UI CCWGG 4 cut(s) 663, 1089, 1554, 1822
Bst4CI ACNGT 6 cut(s) 626, 733, 752, 1393, 1897, 2044
Bst6I CTCTTC 3 cut(s) 171, 477, 1467
BstACI GRCGYC 1 cut(s) 1442
BstAFI CTTAAG 1 cut(s) 1687
BstAPI GCANNNNNTGC 1 cut(s) 293
BstBAI YACGTR 1 cut(s) 1208
BstBI TTCGAA 2 cut(s) 419, 441
BstC8I GCNNGC 2 cut(s) 1143, 1642
BstDEI CTNAG 6 cut(s) 183, 195, 205, 383, 876, 1446
BstDSI CCRYGG 2 cut(s) 267, 1479
BstENI CCTNNNNNAGG 1 cut(s) 1527
BstF5I GGATG 7 cut(s) 637, 842, 893, 977, 1061, 1507, 1873
BstFNI CGCG 1 cut(s) 269
BstHHI GCGC 1 cut(s) 1925
BstKTI GATC 9 cut(s) 352, 369, 472, 805, 1561, 1661, 1948, 1986, 2033
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 9 cut(s) 349, 366, 469, 802, 1558, 1658, 1945, 1983, 2030
BstMWI GCNNNNNNNGC 9 cut(s) 263, 293, 299, 878, 962, 1046, 1226, 1600, 1751
BstNI CCWGG 4 cut(s) 663, 1089, 1554, 1822
BstNSI RCATGY 1 cut(s) 1979
BstSCI CCNGG 5 cut(s) 199, 661, 1087, 1552, 1820
BstSFI CTRYAG 1 cut(s) 1642
BstSLI GKGCMC 3 cut(s) 1133, 1503, 1596
BstUI CGCG 1 cut(s) 269
BstV1I GCAGC 5 cut(s) 241, 280, 286, 807, 1693
BstV2I GAAGAC 1 cut(s) 501
BstX2I RGATCY 2 cut(s) 349, 1983
BstYI RGATCY 2 cut(s) 349, 1983
Bsu15I ATCGAT 1 cut(s) 166
Bsu36I CCTNAGG 2 cut(s) 876, 1446
BsuI GTATCC 2 cut(s) 520, 580
BsuRI GGCC 5 cut(s) 965, 1131, 1215, 1594, 1825
BsuTUI ATCGAT 1 cut(s) 166
BtgI CCRYGG 2 cut(s) 267, 1479
BtgZI GCGATG 1 cut(s) 1122
BtsCI GGATG 7 cut(s) 637, 842, 893, 977, 1061, 1507, 1873
BtsI GCAGTG 1 cut(s) 1826
BtsIMutI CAGTG 5 cut(s) 538, 598, 757, 1572, 1826
Cac8I GCNNGC 2 cut(s) 1143, 1642
CaiI CAGNNNCTG 1 cut(s) 1574
CciI TCATGA 2 cut(s) 1395, 1942
CfoI GCGC 1 cut(s) 1925
Cfr13I GGNCC 6 cut(s) 964, 1129, 1130, 1214, 1592, 1593
Cfr42I CCGCGG 1 cut(s) 270
ClaI ATCGAT 1 cut(s) 166
CseI GACGC 1 cut(s) 1450
Csp6I GTAC 4 cut(s) 361, 1425, 1614, 1934
CspCI CAANNNNNGTGG 2 cut(s) 1945, 1980
CviAII CATG 9 cut(s) 22, 83, 364, 736, 1396, 1480, 1943, 1976, 2048
CviQI GTAC 4 cut(s) 361, 1425, 1614, 1934
DdeI CTNAG 6 cut(s) 183, 195, 205, 383, 876, 1446
DpnI GATC 9 cut(s) 351, 368, 471, 804, 1560, 1660, 1947, 1985, 2032
DpnII GATC 9 cut(s) 349, 366, 469, 802, 1558, 1658, 1945, 1983, 2030
DraIII CACNNNGTG 1 cut(s) 724
Eam1104I CTCTTC 3 cut(s) 171, 477, 1467
EarI CTCTTC 3 cut(s) 171, 477, 1467
Ecl136II GAGCTC 2 cut(s) 742, 1372
Eco130I CCWWGG 2 cut(s) 1479, 1587
Eco24I GRGCYC 4 cut(s) 744, 1133, 1374, 1596
Eco53kI GAGCTC 2 cut(s) 742, 1372
Eco57I CTGAAG 6 cut(s) 501, 513, 746, 1497, 1666, 1716
Eco81I CCTNAGG 2 cut(s) 876, 1446
Eco88I CYCGRG 2 cut(s) 604, 743
EcoICRI GAGCTC 2 cut(s) 742, 1372
EcoNI CCTNNNNNAGG 1 cut(s) 1527
EcoO109I RGGNCCY 3 cut(s) 964, 1129, 1592
EcoRI GAATTC 3 cut(s) 48, 650, 686
EcoRII CCWGG 4 cut(s) 661, 1087, 1552, 1820
EcoT14I CCWWGG 2 cut(s) 1479, 1587
EcoT38I GRGCYC 4 cut(s) 744, 1133, 1374, 1596
ErhI CCWWGG 2 cut(s) 1479, 1587
FaeI CATG 9 cut(s) 25, 86, 367, 739, 1399, 1483, 1946, 1979, 2051
FatI CATG 9 cut(s) 21, 82, 363, 735, 1395, 1479, 1942, 1975, 2047
FbaI TGATCA 1 cut(s) 802
Fnu4HI GCNGC 8 cut(s) 255, 267, 270, 294, 297, 300, 796, 1707
FokI GGATG 7 cut(s) 624, 829, 880, 964, 1048, 1494, 1880
FriOI GRGCYC 4 cut(s) 744, 1133, 1374, 1596
Fsp4HI GCNGC 8 cut(s) 255, 267, 270, 294, 297, 300, 796, 1707
FspBI CTAG 3 cut(s) 464, 1377, 1428
GlaI GCGC 1 cut(s) 1924
GluI GCNGC 8 cut(s) 255, 267, 270, 294, 297, 300, 796, 1707
GsaI CCCAGC 2 cut(s) 1055, 1220
GsuI CTGGAG 2 cut(s) 1209, 1596
HaeIII GGCC 5 cut(s) 965, 1131, 1215, 1594, 1825
HapII CCGG 1 cut(s) 201
HgaI GACGC 1 cut(s) 1450
HhaI GCGC 1 cut(s) 1925
Hin1I GRCGYC 1 cut(s) 1442
Hin1II CATG 9 cut(s) 25, 86, 367, 739, 1399, 1483, 1946, 1979, 2051
Hin6I GCGC 1 cut(s) 1923
HinP1I GCGC 1 cut(s) 1923
HindIII AAGCTT 2 cut(s) 810, 954
HinfI GANTC 6 cut(s) 8, 73, 125, 411, 790, 1471
HpaII CCGG 1 cut(s) 201
HphI GGTGA 2 cut(s) 406, 469
Hpy166II GTNNAC 2 cut(s) 1501, 1916
Hpy8I GTNNAC 2 cut(s) 1501, 1916
HpyCH4III ACNGT 6 cut(s) 626, 733, 752, 1393, 1897, 2044
HpyCH4IV ACGT 2 cut(s) 1123, 1207
HpyCH4V TGCA 9 cut(s) 25, 212, 637, 703, 1501, 1644, 1709, 1765, 1981
HpyF10VI GCNNNNNNNGC 9 cut(s) 263, 293, 299, 878, 962, 1046, 1226, 1600, 1751
HpyF3I CTNAG 6 cut(s) 183, 195, 205, 383, 876, 1446
HpySE526I ACGT 2 cut(s) 1123, 1207
Hsp92I GRCGYC 1 cut(s) 1442
Hsp92II CATG 9 cut(s) 25, 86, 367, 739, 1399, 1483, 1946, 1979, 2051
HspAI GCGC 1 cut(s) 1923
Ksp22I TGATCA 1 cut(s) 802
KspI CCGCGG 1 cut(s) 270
Kzo9I GATC 9 cut(s) 349, 366, 469, 802, 1558, 1658, 1945, 1983, 2030
LmnI GCTCC 6 cut(s) 379, 572, 739, 913, 1577, 1973
Lsp1109I GCAGC 5 cut(s) 241, 280, 286, 807, 1693
LweI GCATC 4 cut(s) 646, 690, 1064, 1229
MaeI CTAG 3 cut(s) 464, 1377, 1428
MaeII ACGT 2 cut(s) 1123, 1207
MaeIII GTNAC 2 cut(s) 1516, 1570
MalI GATC 9 cut(s) 351, 368, 471, 804, 1560, 1660, 1947, 1985, 2032
MboI GATC 9 cut(s) 349, 366, 469, 802, 1558, 1658, 1945, 1983, 2030
MflI RGATCY 2 cut(s) 349, 1983
MhlI GDGCHC 6 cut(s) 744, 750, 1133, 1374, 1503, 1596
MluCI AATT 9 cut(s) 48, 78, 437, 650, 686, 885, 1629, 1785, 1885
MlyI GAGTC 4 cut(s) 2, 405, 799, 1465
MmeI TCCRAC 3 cut(s) 333, 406, 1499
MseI TTAA 3 cut(s) 857, 1688, 2036
MslI CAYNNNNRTG 3 cut(s) 452, 614, 1866
MspA1I CMGCKG 1 cut(s) 269
MspCI CTTAAG 1 cut(s) 1687
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 5 cut(s) 201, 663, 1089, 1554, 1822
Mva1269I GAATGC 2 cut(s) 62, 94
MvaI CCWGG 4 cut(s) 663, 1089, 1554, 1822
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 9 cut(s) 263, 293, 299, 878, 962, 1046, 1226, 1600, 1751
NciI CCSGG 1 cut(s) 201
NcoI CCATGG 1 cut(s) 1479
NdeII GATC 9 cut(s) 349, 366, 469, 802, 1558, 1658, 1945, 1983, 2030
NlaIII CATG 9 cut(s) 25, 86, 367, 739, 1399, 1483, 1946, 1979, 2051
NlaIV GGNNCC 7 cut(s) 882, 966, 1102, 1131, 1593, 1594, 1969
NmuCI GTSAC 1 cut(s) 1570
NspI RCATGY 1 cut(s) 1979
NspV TTCGAA 2 cut(s) 419, 441
PaeR7I CTCGAG 2 cut(s) 604, 743
PagI TCATGA 2 cut(s) 1395, 1942
PctI GAATGC 2 cut(s) 62, 94
PfeI GAWTC 2 cut(s) 73, 125
PflMI CCANNNNNTGG 1 cut(s) 724
PfoI TCCNGGA 1 cut(s) 1087
PkrI GCNGC 8 cut(s) 256, 268, 271, 295, 298, 301, 797, 1708
PleI GAGTC 4 cut(s) 2, 405, 798, 1465
PpsI GAGTC 4 cut(s) 2, 405, 798, 1465
Ppu21I YACGTR 1 cut(s) 1208
PshBI ATTAAT 1 cut(s) 857
PsiI TTATAA 1 cut(s) 1842
Psp124BI GAGCTC 2 cut(s) 744, 1374
Psp6I CCWGG 4 cut(s) 661, 1087, 1552, 1820
PspFI CCCAGC 2 cut(s) 1051, 1216
PspGI CCWGG 4 cut(s) 661, 1087, 1552, 1820
PspN4I GGNNCC 7 cut(s) 882, 966, 1102, 1131, 1593, 1594, 1969
PspOMI GGGCCC 2 cut(s) 1129, 1592
PspPI GGNCC 6 cut(s) 964, 1129, 1130, 1214, 1592, 1593
PspXI VCTCGAGB 2 cut(s) 604, 743
PstI CTGCAG 1 cut(s) 1646
PstNI CAGNNNCTG 1 cut(s) 1574
PsuI RGATCY 2 cut(s) 349, 1983
RsaI GTAC 4 cut(s) 362, 1426, 1615, 1935
RsaNI GTAC 4 cut(s) 361, 1425, 1614, 1934
RseI CAYNNNNRTG 3 cut(s) 452, 614, 1866
SacI GAGCTC 2 cut(s) 744, 1374
SacII CCGCGG 1 cut(s) 270
SaqAI TTAA 3 cut(s) 857, 1688, 2036
SatI GCNGC 8 cut(s) 255, 267, 270, 294, 297, 300, 796, 1707
Sau3AI GATC 9 cut(s) 349, 366, 469, 802, 1558, 1658, 1945, 1983, 2030
Sau96I GGNCC 6 cut(s) 964, 1129, 1130, 1214, 1592, 1593
SchI GAGTC 4 cut(s) 2, 405, 799, 1465
ScrFI CCNGG 5 cut(s) 201, 663, 1089, 1554, 1822
SduI GDGCHC 6 cut(s) 744, 750, 1133, 1374, 1503, 1596
SfaNI GCATC 4 cut(s) 646, 690, 1064, 1229
SfcI CTRYAG 1 cut(s) 1642
Sfr274I CTCGAG 2 cut(s) 604, 743
Sfr303I CCGCGG 1 cut(s) 270
SfuI TTCGAA 2 cut(s) 419, 441
SgrBI CCGCGG 1 cut(s) 270
SlaI CTCGAG 2 cut(s) 604, 743
SmiMI CAYNNNNRTG 3 cut(s) 452, 614, 1866
SmlI CTYRAG 5 cut(s) 544, 604, 743, 1414, 1687
SmoI CTYRAG 5 cut(s) 544, 604, 743, 1414, 1687
SpeI ACTAGT 1 cut(s) 1427
Sse9I AATT 9 cut(s) 48, 78, 437, 650, 686, 885, 1629, 1785, 1885
SsiI CCGC 3 cut(s) 267, 269, 296
SspI AATATT 1 cut(s) 2068
SspMI CTAG 3 cut(s) 464, 1377, 1428
SstI GAGCTC 2 cut(s) 744, 1374
StyD4I CCNGG 5 cut(s) 199, 661, 1087, 1552, 1820
StyI CCWWGG 2 cut(s) 1479, 1587
TaaI ACNGT 6 cut(s) 626, 733, 752, 1393, 1897, 2044
TaiI ACGT 2 cut(s) 1126, 1210
TaqI TCGA 8 cut(s) 11, 66, 166, 419, 441, 605, 744, 1728
TasI AATT 9 cut(s) 48, 78, 437, 650, 686, 885, 1629, 1785, 1885
TatI WGTACW 1 cut(s) 360
TauI GCSGC 3 cut(s) 269, 272, 299
TfiI GAWTC 2 cut(s) 73, 125
Tru1I TTAA 3 cut(s) 857, 1688, 2036
Tru9I TTAA 3 cut(s) 857, 1688, 2036
TscAI CASTG 5 cut(s) 538, 598, 757, 1579, 1833
TseFI GTSAC 1 cut(s) 1570
TseI GCWGC 5 cut(s) 254, 293, 299, 795, 1706
Tsp45I GTSAC 1 cut(s) 1570
TspDTI ATGAA 6 cut(s) 71, 117, 804, 831, 1659, 1941
TspGWI ACGGA 3 cut(s) 404, 1601, 1787
TspRI CASTG 5 cut(s) 538, 598, 757, 1579, 1833
Van91I CCANNNNNTGG 1 cut(s) 724
Vha464I CTTAAG 1 cut(s) 1687
VneI GTGCAC 1 cut(s) 1499
VspI ATTAAT 1 cut(s) 857
XagI CCTNNNNNAGG 1 cut(s) 1527
XapI RAATTY 5 cut(s) 48, 437, 650, 686, 1629
XbaI TCTAGA 1 cut(s) 1376
XceI RCATGY 1 cut(s) 1979
XhoI CTCGAG 2 cut(s) 604, 743
XspI CTAG 3 cut(s) 464, 1377, 1428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.