Rh4CG332900

TPX2 (targeting protein for Xklp2) protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001269
Physical Location & Seq
Forward (+)
59393974 .. 59397240
3267 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG332900.1

Sequence Viewer

Length: 1371 bp
ATGGGTGACTCTTCGTGTCTAATGAGGTCGTTTTCTGGGCCTTCTGAGCGTTCCGGTGAAGTCAAAGAGGGGGGCCTCCTTCGGTGTCTAGGAGAATCAGTCTCATTCGGTAGGTATGCTTCAGAACCTCTTGATTGGGAAAAATGGTCGACATTTTCTCACAATCGGTATCTGGAAGAAGTGGAGAAGTTTTCCAAACCGGGCTCGGTTGCACAGAAGAAAGCCTACTTTGAAGCTCACTACAAAAAGAAGGCTGCTGAGAAAGCAGCAGCTTTGGCTGAGGTCGCAAATACTTGTGATAGCAAAGAACCCGAGTCAGTTTCTATGCACAAGAGTTGTCAAAGCTCCTCTGTCAACATGGAGTTGGCAAATGGAGAGAGCCAGATGGTTGAGGAAAATATAGTTCCAAGTACTGAGGTGGTATACTCAGCTGTTGTGAATGACCACAATCCAGATGTTGAAGGGACTCAATTGGAAAATGCAAAGGTGGATGGAGCAGAGACGGTCATTCAAGAAAGTGTTACTGTGGAGGATGCTATTCAGGTTGAGGTGGAGAATTCTATTCAGGATGAAATGGATACTTCGATTCAGATTCAGATGGAGAGTTCTACTCAAGTTGAAATCTCAAACCAGTTTGAAAATGACCAAAAAGATATTGTTAACGGTGCCACCCAAGAAGAGAAGATGCCTAATAAGGAAGCTGCGAGTGAGGAAAATCTAGCTTCAACAAGCAAGAAAAGATCGGTGAACTCTTCTCCCTCAAGGTTATCTACTCAATGTAGAGCGTCCAAGATCCAATCTTTTCCTGCCAAACAACTTGCTGGTGTTCCAACTGGAAAGAAGAACAATGCCACCCCAAAGAGTAAGAATTCTGAGGCTGACTTAGTTGACAAGAGGAGATCAACAAAAAAATCACTCCACATGTCAATCAATTTCAGCTCTCATGCTGGTGAAACCAGTAAAAGAACTTCGCCGGTCCTGGAGAAGATAAAAAACTTAAGAGTTGATGCTACTGCACTTAATGTGCCTGTTAAAAACTCAGCTTCCCTACAAACTGCAACTAGGGAAAAAGCAGTGAATGATGTTAAAAAATTGCGTCAGAGTACAGGCTTAAAAGCCAAAGATCTGTCTTCTGGATCACAATTGCTAAATAATAAAGTGAGCAAGGTTCCACCGACCCGGAGTCAGTCACAGAAACTCGAGAGGCAGTCTACTACCAGCAAGGTCTTGGACCCAAGCACTCGGCCTTCAGTCAATTCTCACAGCTCTAAGCATGTCACACAGAAATATAACCCAAGGACCAGCCAGTCAGTCACTTCCCTTCCAAAGAAGAATGCTCATGAGAATTCTTCTCCCAATATTCAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

456

Amino Acids

49.86

Weight (kDa)

8.55

Isoelectric Point (pI)

57.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1306
AccB1I GGYRCC 1 cut(s) 665
AccI GTMKAC 3 cut(s) 149, 423, 1211
AclWI GGATC 2 cut(s) 787, 1144
AcsI RAATTY 3 cut(s) 556, 868, 1345
AcuI CTGAAG 2 cut(s) 105, 1233
AfaI GTAC 2 cut(s) 412, 1105
AflII CTTAAG 1 cut(s) 997
AflIII ACRYGT 1 cut(s) 921
AgsI TTSAA 6 cut(s) 233, 461, 512, 620, 638, 726
AhdI GACNNNNNGTC 1 cut(s) 1182
AjnI CCWGG 1 cut(s) 978
AluBI AGCT 9 cut(s) 236, 272, 345, 431, 701, 722, 939, 1043, 1266
AluI AGCT 9 cut(s) 236, 272, 345, 431, 701, 722, 939, 1043, 1266
Alw26I GTCTC 2 cut(s) 106, 494
AlwI GGATC 2 cut(s) 787, 1144
Ama87I CYCGRG 2 cut(s) 311, 1199
AoxI GGCC 3 cut(s) 38, 73, 1244
ApeKI GCWGC 4 cut(s) 254, 266, 269, 701
ApoI RAATTY 3 cut(s) 556, 868, 1345
AspS9I GGNCC 5 cut(s) 38, 73, 976, 1231, 1299
AsuC2I CCSGG 2 cut(s) 201, 1180
AsuHPI GGTGA 4 cut(s) 17, 68, 757, 962
AvaI CYCGRG 2 cut(s) 311, 1199
AvaII GGWCC 3 cut(s) 976, 1231, 1299
BanI GGYRCC 1 cut(s) 665
BanII GRGCYC 1 cut(s) 206
BarI GAAGNNNNNNTAC 6 cut(s) 103, 135, 209, 241, 952, 984
BbsI GAAGAC 1 cut(s) 1122
BbvCI CCTCAGC 1 cut(s) 279
BbvI GCAGC 4 cut(s) 241, 278, 281, 688
BccI CCATC 3 cut(s) 379, 485, 592
BciT130I CCWGG 1 cut(s) 980
BciVI GTATCC 1 cut(s) 571
BcnI CCSGG 2 cut(s) 201, 1180
BcoDI GTCTC 2 cut(s) 106, 494
BfaI CTAG 3 cut(s) 89, 719, 1062
BfrI CTTAAG 1 cut(s) 997
BfuI GTATCC 1 cut(s) 571
BglII AGATCT 1 cut(s) 1123
BisI GCNGC 4 cut(s) 255, 267, 270, 702
BlsI GCNGC 4 cut(s) 256, 268, 271, 703
BmcAI AGTACT 1 cut(s) 412
Bme1390I CCNGG 3 cut(s) 201, 980, 1180
Bme18I GGWCC 3 cut(s) 976, 1231, 1299
BmeRI GACNNNNNGTC 1 cut(s) 1182
BmeT110I CYCGRG 2 cut(s) 311, 1199
BmgT120I GGNCC 5 cut(s) 38, 73, 976, 1231, 1299
BmiI GGNNCC 4 cut(s) 74, 667, 1170, 1233
BmrFI CCNGG 3 cut(s) 201, 980, 1180
BmsI GCATC 3 cut(s) 523, 675, 997
BpiI GAAGAC 1 cut(s) 1122
BplI GAGNNNNNCTC 2 cut(s) 595, 627
BpmI CTGGAG 1 cut(s) 1001
Bpu10I CCTNAGC 1 cut(s) 279
BpuEI CTTGAG 2 cut(s) 597, 745
BpuMI CCSGG 2 cut(s) 201, 1180
BsaJI CCNNGG 1 cut(s) 1295
BsaWI WCCGGW 1 cut(s) 53
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bse118I RCCGGY 1 cut(s) 973
Bse1I ACTGG 4 cut(s) 631, 838, 957, 1306
BseBI CCWGG 1 cut(s) 980
BseDI CCNNGG 1 cut(s) 1295
BseGI GGATG 3 cut(s) 496, 538, 574
BseMII CTCAG 7 cut(s) 36, 249, 270, 405, 441, 864, 1053
BseNI ACTGG 4 cut(s) 631, 838, 957, 1306
BseRI GAGGAG 2 cut(s) 337, 910
BseXI GCAGC 4 cut(s) 241, 278, 281, 688
BsgI GTGCAG 1 cut(s) 999
BshFI GGCC 3 cut(s) 40, 75, 1246
BshNI GGYRCC 1 cut(s) 665
BsiHKCI CYCGRG 2 cut(s) 311, 1199
BsiSI CCGG 4 cut(s) 54, 200, 974, 1180
BslFI GGGAC 1 cut(s) 478
BsmAI GTCTC 2 cut(s) 106, 494
BsmBI CGTCTC 1 cut(s) 494
BsmFI GGGAC 1 cut(s) 478
BsmI GAATGC 1 cut(s) 1339
BsnI GGCC 3 cut(s) 40, 75, 1246
BsoBI CYCGRG 2 cut(s) 311, 1199
Bsp1286I GDGCHC 1 cut(s) 206
Bsp143I GATC 5 cut(s) 740, 792, 899, 1123, 1136
BspANI GGCC 3 cut(s) 40, 75, 1246
BspCNI CTCAG 7 cut(s) 37, 250, 271, 406, 440, 865, 1052
BspHI TCATGA 1 cut(s) 1339
BspLI GGNNCC 4 cut(s) 74, 667, 1170, 1233
BspPI GGATC 2 cut(s) 787, 1144
BspT107I GGYRCC 1 cut(s) 665
BspTI CTTAAG 1 cut(s) 997
BsrFI RCCGGY 1 cut(s) 973
BsrI ACTGG 4 cut(s) 631, 838, 957, 1306
BssAI RCCGGY 1 cut(s) 973
BssECI CCNNGG 1 cut(s) 1295
BssMI GATC 5 cut(s) 740, 792, 899, 1123, 1136
BssNAI GTATAC 1 cut(s) 424
BssT1I CCWWGG 1 cut(s) 1295
Bst1107I GTATAC 1 cut(s) 424
Bst2UI CCWGG 1 cut(s) 980
Bst4CI ACNGT 3 cut(s) 505, 526, 665
Bst6I CTCTTC 3 cut(s) 16, 672, 757
BstAFI CTTAAG 1 cut(s) 997
BstDEI CTNAG 9 cut(s) 45, 258, 279, 414, 427, 873, 883, 1039, 1269
BstF5I GGATG 3 cut(s) 496, 538, 574
BstKTI GATC 5 cut(s) 743, 795, 902, 1126, 1139
BstMAI GTCTC 2 cut(s) 106, 494
BstMBI GATC 5 cut(s) 740, 792, 899, 1123, 1136
BstMWI GCNNNNNNNGC 4 cut(s) 46, 263, 275, 284
BstNI CCWGG 1 cut(s) 980
BstNSI RCATGY 2 cut(s) 925, 1277
BstSCI CCNGG 3 cut(s) 199, 978, 1178
BstV1I GCAGC 4 cut(s) 241, 278, 281, 688
BstV2I GAAGAC 1 cut(s) 1122
BstX2I RGATCY 2 cut(s) 792, 1123
BstYI RGATCY 2 cut(s) 792, 1123
BstZ17I GTATAC 1 cut(s) 424
BsuI GTATCC 1 cut(s) 571
BsuRI GGCC 3 cut(s) 40, 75, 1246
BtsCI GGATG 3 cut(s) 496, 538, 574
BtsI GCAGTG 1 cut(s) 1080
BtsIMutI CAGTG 1 cut(s) 1080
CciI TCATGA 1 cut(s) 1339
Cfr10I RCCGGY 1 cut(s) 973
Cfr13I GGNCC 5 cut(s) 38, 73, 976, 1231, 1299
CseI GACGC 2 cut(s) 774, 1085
Csp6I GTAC 2 cut(s) 411, 1104
CviAII CATG 5 cut(s) 358, 922, 944, 1274, 1340
CviQI GTAC 2 cut(s) 411, 1104
DdeI CTNAG 9 cut(s) 45, 258, 279, 414, 427, 873, 883, 1039, 1269
DpnI GATC 5 cut(s) 742, 794, 901, 1125, 1138
DpnII GATC 5 cut(s) 740, 792, 899, 1123, 1136
DrdI GACNNNNNNGTC 1 cut(s) 1306
DriI GACNNNNNGTC 1 cut(s) 1182
DseDI GACNNNNNNGTC 1 cut(s) 1306
Eam1104I CTCTTC 3 cut(s) 16, 672, 757
Eam1105I GACNNNNNGTC 1 cut(s) 1182
EarI CTCTTC 3 cut(s) 16, 672, 757
Eco130I CCWWGG 1 cut(s) 1295
Eco24I GRGCYC 1 cut(s) 206
Eco47I GGWCC 3 cut(s) 976, 1231, 1299
Eco57I CTGAAG 2 cut(s) 105, 1233
Eco88I CYCGRG 2 cut(s) 311, 1199
EcoO109I RGGNCCY 1 cut(s) 73
EcoRI GAATTC 3 cut(s) 556, 868, 1345
EcoRII CCWGG 1 cut(s) 978
EcoT14I CCWWGG 1 cut(s) 1295
EcoT38I GRGCYC 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 1295
Esp3I CGTCTC 1 cut(s) 494
FaeI CATG 5 cut(s) 361, 925, 947, 1277, 1343
FaqI GGGAC 1 cut(s) 478
FatI CATG 5 cut(s) 357, 921, 943, 1273, 1339
FblI GTMKAC 3 cut(s) 149, 423, 1211
Fnu4HI GCNGC 4 cut(s) 255, 267, 270, 702
FokI GGATG 3 cut(s) 503, 545, 581
FriOI GRGCYC 1 cut(s) 206
Fsp4HI GCNGC 4 cut(s) 255, 267, 270, 702
FspBI CTAG 3 cut(s) 89, 719, 1062
GluI GCNGC 4 cut(s) 255, 267, 270, 702
GsuI CTGGAG 1 cut(s) 1001
HaeIII GGCC 3 cut(s) 40, 75, 1246
HapII CCGG 4 cut(s) 54, 200, 974, 1180
HgaI GACGC 2 cut(s) 774, 1085
Hin1II CATG 5 cut(s) 361, 925, 947, 1277, 1343
HincII GTYRAC 4 cut(s) 150, 355, 661, 889
HindII GTYRAC 4 cut(s) 150, 355, 661, 889
HinfI GANTC 7 cut(s) 8, 95, 314, 466, 586, 592, 1183
HpaI GTTAAC 1 cut(s) 661
HpaII CCGG 4 cut(s) 54, 200, 974, 1180
HphI GGTGA 4 cut(s) 17, 68, 757, 962
Hpy166II GTNNAC 7 cut(s) 150, 355, 424, 661, 748, 889, 1212
Hpy188I TCNGA 7 cut(s) 46, 124, 591, 597, 874, 1101, 1365
Hpy188III TCNNGA 8 cut(s) 131, 173, 452, 512, 566, 1134, 1201, 1340
Hpy8I GTNNAC 7 cut(s) 150, 355, 424, 661, 748, 889, 1212
HpyAV CCTTC 6 cut(s) 51, 89, 244, 455, 1257, 1331
HpyCH4III ACNGT 3 cut(s) 505, 526, 665
HpyCH4V TGCA 5 cut(s) 212, 328, 482, 1016, 1058
HpyF10VI GCNNNNNNNGC 4 cut(s) 46, 263, 275, 284
HpyF3I CTNAG 9 cut(s) 45, 258, 279, 414, 427, 873, 883, 1039, 1269
Hsp92II CATG 5 cut(s) 361, 925, 947, 1277, 1343
KspAI GTTAAC 1 cut(s) 661
Kzo9I GATC 5 cut(s) 740, 792, 899, 1123, 1136
LmnI GCTCC 2 cut(s) 350, 494
Lsp1109I GCAGC 4 cut(s) 241, 278, 281, 688
LweI GCATC 3 cut(s) 523, 675, 997
MaeI CTAG 3 cut(s) 89, 719, 1062
MaeIII GTNAC 5 cut(s) 5, 520, 1188, 1276, 1312
MalI GATC 5 cut(s) 742, 794, 901, 1125, 1138
MboI GATC 5 cut(s) 740, 792, 899, 1123, 1136
MfeI CAATTG 2 cut(s) 470, 1142
MflI RGATCY 2 cut(s) 792, 1123
MhlI GDGCHC 1 cut(s) 206
MluCI AATT 8 cut(s) 470, 556, 868, 931, 1091, 1142, 1255, 1345
MlyI GAGTC 4 cut(s) 2, 323, 460, 1192
MmeI TCCRAC 1 cut(s) 854
MseI TTAA 6 cut(s) 660, 998, 1020, 1032, 1086, 1112
MslI CAYNNNNRTG 1 cut(s) 948
MspA1I CMGCKG 1 cut(s) 431
MspCI CTTAAG 1 cut(s) 997
MspI CCGG 4 cut(s) 54, 200, 974, 1180
MspR9I CCNGG 3 cut(s) 201, 980, 1180
MunI CAATTG 2 cut(s) 470, 1142
Mva1269I GAATGC 1 cut(s) 1339
MvaI CCWGG 1 cut(s) 980
MwoI GCNNNNNNNGC 4 cut(s) 46, 263, 275, 284
NciI CCSGG 2 cut(s) 201, 1180
NdeII GATC 5 cut(s) 740, 792, 899, 1123, 1136
NlaIII CATG 5 cut(s) 361, 925, 947, 1277, 1343
NlaIV GGNNCC 4 cut(s) 74, 667, 1170, 1233
NmeAIII GCCGAG 1 cut(s) 1222
NmuCI GTSAC 4 cut(s) 5, 1188, 1276, 1312
NspI RCATGY 2 cut(s) 925, 1277
PaeR7I CTCGAG 1 cut(s) 1199
PagI TCATGA 1 cut(s) 1339
PciI ACATGT 1 cut(s) 921
PctI GAATGC 1 cut(s) 1339
PfeI GAWTC 3 cut(s) 95, 586, 592
PfoI TCCNGGA 1 cut(s) 978
PkrI GCNGC 4 cut(s) 256, 268, 271, 703
PleI GAGTC 4 cut(s) 2, 322, 460, 1191
PpsI GAGTC 4 cut(s) 2, 322, 460, 1191
PscI ACATGT 1 cut(s) 921
Psp6I CCWGG 1 cut(s) 978
PspGI CCWGG 1 cut(s) 978
PspN4I GGNNCC 4 cut(s) 74, 667, 1170, 1233
PspPI GGNCC 5 cut(s) 38, 73, 976, 1231, 1299
PsuI RGATCY 2 cut(s) 792, 1123
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 2 cut(s) 412, 1105
RsaNI GTAC 2 cut(s) 411, 1104
RseI CAYNNNNRTG 1 cut(s) 948
SalI GTCGAC 1 cut(s) 148
SaqAI TTAA 6 cut(s) 660, 998, 1020, 1032, 1086, 1112
SatI GCNGC 4 cut(s) 255, 267, 270, 702
Sau3AI GATC 5 cut(s) 740, 792, 899, 1123, 1136
Sau96I GGNCC 5 cut(s) 38, 73, 976, 1231, 1299
ScaI AGTACT 1 cut(s) 412
SchI GAGTC 4 cut(s) 2, 323, 460, 1192
ScrFI CCNGG 3 cut(s) 201, 980, 1180
SduI GDGCHC 1 cut(s) 206
SfaNI GCATC 3 cut(s) 523, 675, 997
Sfr274I CTCGAG 1 cut(s) 1199
SinI GGWCC 3 cut(s) 976, 1231, 1299
SlaI CTCGAG 1 cut(s) 1199
SmiMI CAYNNNNRTG 1 cut(s) 948
SmlI CTYRAG 4 cut(s) 612, 760, 997, 1199
SmoI CTYRAG 4 cut(s) 612, 760, 997, 1199
Sse9I AATT 8 cut(s) 470, 556, 868, 931, 1091, 1142, 1255, 1345
SspI AATATT 1 cut(s) 1360
SspMI CTAG 3 cut(s) 89, 719, 1062
StyD4I CCNGG 3 cut(s) 199, 978, 1178
StyI CCWWGG 1 cut(s) 1295
TaaI ACNGT 3 cut(s) 505, 526, 665
TaqI TCGA 3 cut(s) 149, 584, 1200
TasI AATT 8 cut(s) 470, 556, 868, 931, 1091, 1142, 1255, 1345
TatI WGTACW 2 cut(s) 410, 1103
TfiI GAWTC 3 cut(s) 95, 586, 592
Tru1I TTAA 6 cut(s) 660, 998, 1020, 1032, 1086, 1112
Tru9I TTAA 6 cut(s) 660, 998, 1020, 1032, 1086, 1112
TscAI CASTG 1 cut(s) 1080
TseFI GTSAC 4 cut(s) 5, 1188, 1276, 1312
TseI GCWGC 4 cut(s) 254, 266, 269, 701
Tsp45I GTSAC 4 cut(s) 5, 1188, 1276, 1312
TspDTI ATGAA 1 cut(s) 585
TspRI CASTG 1 cut(s) 1080
Vha464I CTTAAG 1 cut(s) 997
VpaK11BI GGWCC 3 cut(s) 976, 1231, 1299
XapI RAATTY 3 cut(s) 556, 868, 1345
XceI RCATGY 2 cut(s) 925, 1277
XcmI CCANNNNNNNNNTGG 1 cut(s) 1225
XhoI CTCGAG 1 cut(s) 1199
XmiI GTMKAC 3 cut(s) 149, 423, 1211
XspI CTAG 3 cut(s) 89, 719, 1062
ZrmI AGTACT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.