pycom13g09370

TPX2 (targeting protein for Xklp2) protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001269
Physical Location & Seq
Reverse (-)
6284772 .. 6288101
3330 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g09370.3

Sequence Viewer

Length: 1695 bp
ATGGGTGAATCGGCCTGCCTGATGAGATCATTATACGACCCTTCTGAGTGTTCCACTGAAGTCAAAGAGGGTGACCCCCTTCGTTGCCTAGGAGAATCGATCTCCTTCGGTAGGTATATGTCAGAACCTCTTGCTTGGGAGAAATGGTCAGCATTTTCCCACAATCGATACTTAGAAGAAGTCGAGAAGTTCTCCAAGCCCGGCTCAGTTGCTCAGAAGAAGGCTTATTTTGAAGCTCACTACAAGAAAAAAGCGGCCGAGAAAGCAGCAGCCTTGCTTGAGGTGACAAATGCTGGTGCCTGTAATGTTACAGCATCATCGGTAACTATGTACGGAAATTGTGAGAGCTCCTCCATGGACATAGAATCGGCAAATGGAGGGAGCCAGATGGTCGTTGATAAAGAACAGGAAAATGTTGCTCCAAATTCTGAGGTAGAAGTATACTCAGCTGATATGAATAGCCATAATCAAAATGTTGAAGGGAATCATTTGGATATCGCCAAGGTGGACGGAGCAGAAGCAGTGATTGAAGCAAGTGTGAATTTGGAAAGTCCTATTCACGTTGAAATATCGAACCAGTTTGAAAATGGCAAGGACCAAAATGAGATTGTTGCCACCCTAGAAGAGAAGATGCCAAATACGATCCTCCAGGAAGCTTCCGGTGAGGAGAATCTTGCTTCAATGAGCAAGAAAAGATTGATGAACTCTTCCCCAAGGTTATCCACAAAAGGTAGAGCACCCAAGGTACCACCGTCTCCTGCCAAACAAGCAATTCATGTGCAAACCATAAACAGGAAAAATGCCACTCAAATGGGTAAGAAGGTTTCATCAGACTTAGTTGACAAGAGGAGTTTGACTGCAAAATCTCTCCACATGTCAATGCATTTCACTTCTCATGGGGGCGAAAATGAAGCCAGTAAAAGAACTTCACCAGTTGTTGAGAAGACCAGAAATTCGAGAAGTAACACAACTAAACTTGATATCTCCATAAATAATCCGGCTTCTCATCGACGAACTGCTATTAGGGCATCTGTTGGCGGGGTATTAATGCAGTCTTCAGTAGACCCATGGTCCAAAGATAGAAGGGCAAAAACATTACTGAATAAGTCAGTAGCTGGACCAATTGGAGATGGGAAATGGATATCCTCCTCAAAAGACCAATCTTCTACCGCAAATGGAAGTAAACCACGACCCCCTATTGTATCATGTCCTTTTAGATTGAAGAGTGAAGAAAGAGCAGAAAAGCGTAAAGAGTTCTATCAGAGGCTGGAAGAGAAGAAAAATGCCAAGGAGGCTGAACATAAGCTGCTGCAACTGAGATCTAATAAAAGGAGCAAGTCGTATAAAACATGTGTGACGGGGCAATGCGAGTTGAATCCTGAAAAAGCAGTGGGTGATGTTAATAAACTACACCAGAGCACTGCCTTAAAAGCGAAACAAAACCAAGATCTGTCCTCAGGACTACAATTGTCAAGTAATCACTTGAACAAGATTCCTATGTCACAGTCTCGGTCACCCAAACTTGGGAGGCACTCTACTGCCATCAAAATTCTGGACTCAAGCTCTCGACCTTTTGTCAATTCTCACAGCTCCAAGTGTGTCACACAAAAATACAATTTGAGCACATATCGAATGGTGCCTTCTCTTTCAAAGAAGAATGCTCACGAGAATTCTCCTCCCAATATCCTGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

565

Amino Acids

62.06

Weight (kDa)

9.34

Isoelectric Point (pI)

60.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 745
AccB1I GGYRCC 3 cut(s) 296, 745, 1636
AccI GTMKAC 2 cut(s) 441, 1062
AciI CCGC 3 cut(s) 254, 1038, 1170
AclWI GGATC 1 cut(s) 637
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 5 cut(s) 424, 541, 952, 1548, 1669
AcuI CTGAAG 2 cut(s) 78, 1041
AfaI GTAC 2 cut(s) 332, 747
AfiI CCNNNNNNNGG 4 cut(s) 111, 792, 1523, 1524
AflIII ACRYGT 2 cut(s) 873, 1349
AhdI GACNNNNNGTC 2 cut(s) 1069, 1574
AjnI CCWGG 1 cut(s) 648
AluBI AGCT 8 cut(s) 236, 348, 449, 656, 1115, 1306, 1563, 1590
AluI AGCT 8 cut(s) 236, 348, 449, 656, 1115, 1306, 1563, 1590
Alw21I GWGCWC 4 cut(s) 350, 739, 1421, 1625
Alw26I GTCTC 2 cut(s) 759, 1512
AlwI GGATC 1 cut(s) 637
AlwNI CAGNNNCTG 2 cut(s) 1115, 1267
AoxI GGCC 2 cut(s) 12, 255
ApeKI GCWGC 4 cut(s) 266, 269, 1306, 1309
ApoI RAATTY 5 cut(s) 424, 541, 952, 1548, 1669
ArsI GACNNNNNNTTYG 2 cut(s) 937, 969
AseI ATTAAT 1 cut(s) 1046
Asp718I GGTACC 1 cut(s) 745
AspA2I CCTAGG 1 cut(s) 88
AspS9I GGNCC 3 cut(s) 595, 1071, 1118
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 7 cut(s) 17, 83, 295, 674, 921, 1406, 1506
AvaII GGWCC 3 cut(s) 595, 1071, 1118
AvrII CCTAGG 1 cut(s) 88
AxyI CCTNAGG 1 cut(s) 1456
BaeI ACNNNNGTAYC 2 cut(s) 737, 770
BanI GGYRCC 3 cut(s) 296, 745, 1636
BanII GRGCYC 1 cut(s) 350
BarI GAAGNNNNNNTAC 2 cut(s) 910, 942
BauI CACGAG 1 cut(s) 1664
BbsI GAAGAC 2 cut(s) 950, 1047
Bbv12I GWGCWC 4 cut(s) 350, 739, 1421, 1625
BbvI GCAGC 4 cut(s) 278, 281, 1293, 1296
BccI CCATC 3 cut(s) 382, 1124, 1550
BciT130I CCWGG 1 cut(s) 650
BcnI CCSGG 1 cut(s) 201
BcoDI GTCTC 2 cut(s) 759, 1512
BfaI CTAG 2 cut(s) 89, 620
BglI GCCNNNNNGGC 1 cut(s) 1292
BglII AGATCT 2 cut(s) 1319, 1447
BisI GCNGC 5 cut(s) 255, 267, 270, 1307, 1310
BlnI CCTAGG 1 cut(s) 88
BlsI GCNGC 5 cut(s) 256, 268, 271, 1308, 1311
Bme1390I CCNGG 2 cut(s) 201, 650
Bme18I GGWCC 3 cut(s) 595, 1071, 1118
BmeRI GACNNNNNGTC 2 cut(s) 1069, 1574
BmgT120I GGNCC 3 cut(s) 595, 1071, 1118
BmiI GGNNCC 4 cut(s) 298, 383, 747, 1638
BmrFI CCNGG 2 cut(s) 201, 650
BmsI GCATC 3 cut(s) 323, 621, 1037
BpiI GAAGAC 2 cut(s) 950, 1047
BplI GAGNNNNNCTC 4 cut(s) 176, 208, 335, 367
BpmI CTGGAG 1 cut(s) 632
BpuEI CTTGAG 2 cut(s) 299, 1543
BpuMI CCSGG 1 cut(s) 201
Bsa29I ATCGAT 2 cut(s) 98, 166
BsaJI CCNNGG 7 cut(s) 88, 354, 501, 713, 741, 1067, 1287
BsaWI WCCGGW 1 cut(s) 659
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 4 cut(s) 111, 792, 1523, 1524
Bse1I ACTGG 3 cut(s) 577, 915, 932
Bse21I CCTNAGG 1 cut(s) 1456
Bse3DI GCAATG 1 cut(s) 1370
BseBI CCWGG 1 cut(s) 650
BseCI ATCGAT 2 cut(s) 98, 166
BseDI CCNNGG 7 cut(s) 88, 354, 501, 713, 741, 1067, 1287
BseLI CCNNNNNNNGG 4 cut(s) 111, 792, 1523, 1524
BseMI GCAATG 1 cut(s) 1370
BseMII CTCAG 8 cut(s) 36, 219, 227, 420, 459, 1307, 1470, 1679
BseNI ACTGG 3 cut(s) 577, 915, 932
BseRI GAGGAG 5 cut(s) 340, 680, 862, 1138, 1665
BseX3I CGGCCG 1 cut(s) 255
BseXI GCAGC 4 cut(s) 278, 281, 1293, 1296
Bsh1285I CGRYCG 1 cut(s) 258
BshFI GGCC 2 cut(s) 14, 257
BshNI GGYRCC 3 cut(s) 296, 745, 1636
BshVI ATCGAT 2 cut(s) 98, 166
BsiEI CGRYCG 1 cut(s) 258
BsiHKAI GWGCWC 4 cut(s) 350, 739, 1421, 1625
BsiSI CCGG 3 cut(s) 201, 660, 998
BslI CCNNNNNNNGG 4 cut(s) 111, 792, 1523, 1524
BsmAI GTCTC 2 cut(s) 759, 1512
BsmBI CGTCTC 1 cut(s) 759
BsmI GAATGC 1 cut(s) 1663
BsnI GGCC 2 cut(s) 14, 257
Bsp1286I GDGCHC 4 cut(s) 350, 739, 1421, 1625
Bsp143I GATC 5 cut(s) 26, 99, 642, 1319, 1447
Bsp19I CCATGG 2 cut(s) 354, 1067
BspACI CCGC 3 cut(s) 254, 1038, 1170
BspANI GGCC 2 cut(s) 14, 257
BspCNI CTCAG 8 cut(s) 37, 218, 226, 421, 458, 1308, 1469, 1680
BspDI ATCGAT 2 cut(s) 98, 166
BspLI GGNNCC 4 cut(s) 298, 383, 747, 1638
BspPI GGATC 1 cut(s) 637
BspT107I GGYRCC 3 cut(s) 296, 745, 1636
BsrDI GCAATG 1 cut(s) 1370
BsrI ACTGG 3 cut(s) 577, 915, 932
BssECI CCNNGG 7 cut(s) 88, 354, 501, 713, 741, 1067, 1287
BssMI GATC 5 cut(s) 26, 99, 642, 1319, 1447
BssNAI GTATAC 1 cut(s) 442
BssSI CACGAG 1 cut(s) 1664
BssT1I CCWWGG 7 cut(s) 88, 354, 501, 713, 741, 1067, 1287
Bst1107I GTATAC 1 cut(s) 442
Bst2BI CACGAG 1 cut(s) 1664
Bst2UI CCWGG 1 cut(s) 650
Bst4CI ACNGT 2 cut(s) 753, 1506
Bst6I CTCTTC 4 cut(s) 618, 712, 1217, 1266
BstC8I GCNNGC 1 cut(s) 16
BstDSI CCRYGG 2 cut(s) 354, 1067
BstEII GGTNACC 2 cut(s) 71, 1512
BstENI CCTNNNNNAGG 1 cut(s) 109
BstKTI GATC 5 cut(s) 29, 102, 645, 1322, 1450
BstMAI GTCTC 2 cut(s) 759, 1512
BstMBI GATC 5 cut(s) 26, 99, 642, 1319, 1447
BstMCI CGRYCG 1 cut(s) 258
BstMWI GCNNNNNNNGC 5 cut(s) 263, 767, 1025, 1292, 1430
BstNI CCWGG 1 cut(s) 650
BstNSI RCATGY 2 cut(s) 877, 1353
BstPI GGTNACC 2 cut(s) 71, 1512
BstSCI CCNGG 2 cut(s) 199, 648
BstV1I GCAGC 4 cut(s) 278, 281, 1293, 1296
BstV2I GAAGAC 2 cut(s) 950, 1047
BstX2I RGATCY 2 cut(s) 1319, 1447
BstXI CCANNNNNNTGG 1 cut(s) 811
BstYI RGATCY 2 cut(s) 1319, 1447
BstZ17I GTATAC 1 cut(s) 442
BstZI CGGCCG 1 cut(s) 255
Bsu15I ATCGAT 2 cut(s) 98, 166
Bsu36I CCTNAGG 1 cut(s) 1456
BsuRI GGCC 2 cut(s) 14, 257
BsuTUI ATCGAT 2 cut(s) 98, 166
BtgI CCRYGG 2 cut(s) 354, 1067
BtsI GCAGTG 3 cut(s) 528, 1395, 1419
BtsIMutI CAGTG 4 cut(s) 54, 528, 1395, 1419
Cac8I GCNNGC 1 cut(s) 16
CaiI CAGNNNCTG 2 cut(s) 1115, 1267
Cfr13I GGNCC 3 cut(s) 595, 1071, 1118
ClaI ATCGAT 2 cut(s) 98, 166
Csp6I GTAC 2 cut(s) 331, 746
CviAII CATG 7 cut(s) 355, 776, 874, 896, 1068, 1206, 1350
CviQI GTAC 2 cut(s) 331, 746
DpnI GATC 5 cut(s) 28, 101, 644, 1321, 1449
DpnII GATC 5 cut(s) 26, 99, 642, 1319, 1447
DriI GACNNNNNGTC 2 cut(s) 1069, 1574
EaeI YGGCCR 1 cut(s) 255
EagI CGGCCG 1 cut(s) 255
Eam1104I CTCTTC 4 cut(s) 618, 712, 1217, 1266
Eam1105I GACNNNNNGTC 2 cut(s) 1069, 1574
EarI CTCTTC 4 cut(s) 618, 712, 1217, 1266
Ecl136II GAGCTC 1 cut(s) 348
EclXI CGGCCG 1 cut(s) 255
Eco130I CCWWGG 7 cut(s) 88, 354, 501, 713, 741, 1067, 1287
Eco24I GRGCYC 1 cut(s) 350
Eco32I GATATC 3 cut(s) 496, 982, 1143
Eco47I GGWCC 3 cut(s) 595, 1071, 1118
Eco52I CGGCCG 1 cut(s) 255
Eco53kI GAGCTC 1 cut(s) 348
Eco57I CTGAAG 2 cut(s) 78, 1041
Eco81I CCTNAGG 1 cut(s) 1456
Eco91I GGTNACC 2 cut(s) 71, 1512
EcoICRI GAGCTC 1 cut(s) 348
EcoNI CCTNNNNNAGG 1 cut(s) 109
EcoO65I GGTNACC 2 cut(s) 71, 1512
EcoRI GAATTC 1 cut(s) 1669
EcoRII CCWGG 1 cut(s) 648
EcoRV GATATC 3 cut(s) 496, 982, 1143
EcoT14I CCWWGG 7 cut(s) 88, 354, 501, 713, 741, 1067, 1287
EcoT22I ATGCAT 1 cut(s) 885
EcoT38I GRGCYC 1 cut(s) 350
ErhI CCWWGG 7 cut(s) 88, 354, 501, 713, 741, 1067, 1287
Esp3I CGTCTC 1 cut(s) 759
FaeI CATG 7 cut(s) 358, 779, 877, 899, 1071, 1209, 1353
FatI CATG 7 cut(s) 354, 775, 873, 895, 1067, 1205, 1349
FauI CCCGC 1 cut(s) 1031
FblI GTMKAC 2 cut(s) 441, 1062
Fnu4HI GCNGC 5 cut(s) 255, 267, 270, 1307, 1310
FriOI GRGCYC 1 cut(s) 350
Fsp4HI GCNGC 5 cut(s) 255, 267, 270, 1307, 1310
FspBI CTAG 2 cut(s) 89, 620
GluI GCNGC 5 cut(s) 255, 267, 270, 1307, 1310
GsuI CTGGAG 1 cut(s) 632
HaeIII GGCC 2 cut(s) 14, 257
HapII CCGG 3 cut(s) 201, 660, 998
Hin1II CATG 7 cut(s) 358, 779, 877, 899, 1071, 1209, 1353
HincII GTYRAC 1 cut(s) 841
HindII GTYRAC 1 cut(s) 841
HindIII AAGCTT 1 cut(s) 654
HinfI GANTC 8 cut(s) 8, 95, 365, 484, 670, 1375, 1492, 1556
HpaII CCGG 3 cut(s) 201, 660, 998
HphI GGTGA 7 cut(s) 17, 83, 295, 674, 921, 1406, 1506
Hpy166II GTNNAC 5 cut(s) 442, 508, 841, 1063, 1184
Hpy188I TCNGA 6 cut(s) 46, 124, 216, 430, 832, 1263
Hpy188III TCNNGA 8 cut(s) 184, 957, 1379, 1458, 1553, 1566, 1664, 1687
Hpy8I GTNNAC 5 cut(s) 442, 508, 841, 1063, 1184
Hpy99I CGWCG 1 cut(s) 1014
HpyAV CCTTC 8 cut(s) 51, 89, 115, 214, 473, 814, 1077, 1650
HpyCH4III ACNGT 2 cut(s) 753, 1506
HpyCH4IV ACGT 1 cut(s) 561
HpyCH4V TGCA 5 cut(s) 781, 860, 883, 1051, 1312
HpyF10VI GCNNNNNNNGC 5 cut(s) 263, 767, 1025, 1292, 1430
HpySE526I ACGT 1 cut(s) 561
Hsp92II CATG 7 cut(s) 358, 779, 877, 899, 1071, 1209, 1353
KpnI GGTACC 1 cut(s) 749
Kzo9I GATC 5 cut(s) 26, 99, 642, 1319, 1447
LmnI GCTCC 6 cut(s) 353, 381, 424, 512, 1332, 1595
Lsp1109I GCAGC 4 cut(s) 278, 281, 1293, 1296
LweI GCATC 3 cut(s) 323, 621, 1037
MaeI CTAG 2 cut(s) 89, 620
MaeII ACGT 1 cut(s) 561
MaeIII GTNAC 9 cut(s) 71, 283, 307, 322, 962, 1354, 1500, 1512, 1600
MalI GATC 5 cut(s) 28, 101, 644, 1321, 1449
MboI GATC 5 cut(s) 26, 99, 642, 1319, 1447
MfeI CAATTG 2 cut(s) 1122, 1466
MflI RGATCY 2 cut(s) 1319, 1447
MhlI GDGCHC 4 cut(s) 350, 739, 1421, 1625
MlyI GAGTC 1 cut(s) 1550
Mph1103I ATGCAT 1 cut(s) 885
MseI TTAA 3 cut(s) 1046, 1401, 1427
MslI CAYNNNNRTG 2 cut(s) 809, 878
MspA1I CMGCKG 1 cut(s) 449
MspI CCGG 3 cut(s) 201, 660, 998
MspR9I CCNGG 2 cut(s) 201, 650
MunI CAATTG 2 cut(s) 1122, 1466
Mva1269I GAATGC 1 cut(s) 1663
MvaI CCWGG 1 cut(s) 650
MwoI GCNNNNNNNGC 5 cut(s) 263, 767, 1025, 1292, 1430
NciI CCSGG 1 cut(s) 201
NcoI CCATGG 2 cut(s) 354, 1067
NdeII GATC 5 cut(s) 26, 99, 642, 1319, 1447
NlaIII CATG 7 cut(s) 358, 779, 877, 899, 1071, 1209, 1353
NlaIV GGNNCC 4 cut(s) 298, 383, 747, 1638
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 6 cut(s) 71, 283, 1354, 1500, 1512, 1600
NsiI ATGCAT 1 cut(s) 885
NspI RCATGY 2 cut(s) 877, 1353
PciI ACATGT 2 cut(s) 873, 1349
PctI GAATGC 1 cut(s) 1663
PfeI GAWTC 7 cut(s) 8, 95, 365, 484, 670, 1375, 1492
PfoI TCCNGGA 1 cut(s) 648
PkrI GCNGC 5 cut(s) 256, 268, 271, 1308, 1311
PleI GAGTC 1 cut(s) 1550
PpsI GAGTC 1 cut(s) 1550
PscI ACATGT 2 cut(s) 873, 1349
PshBI ATTAAT 1 cut(s) 1046
Psp124BI GAGCTC 1 cut(s) 350
Psp6I CCWGG 1 cut(s) 648
PspEI GGTNACC 2 cut(s) 71, 1512
PspGI CCWGG 1 cut(s) 648
PspN4I GGNNCC 4 cut(s) 298, 383, 747, 1638
PspPI GGNCC 3 cut(s) 595, 1071, 1118
PstNI CAGNNNCTG 2 cut(s) 1115, 1267
PsuI RGATCY 2 cut(s) 1319, 1447
PvuII CAGCTG 1 cut(s) 449
RsaI GTAC 2 cut(s) 332, 747
RsaNI GTAC 2 cut(s) 331, 746
RseI CAYNNNNRTG 2 cut(s) 809, 878
SacI GAGCTC 1 cut(s) 350
SaqAI TTAA 3 cut(s) 1046, 1401, 1427
SatI GCNGC 5 cut(s) 255, 267, 270, 1307, 1310
Sau3AI GATC 5 cut(s) 26, 99, 642, 1319, 1447
Sau96I GGNCC 3 cut(s) 595, 1071, 1118
SchI GAGTC 1 cut(s) 1550
ScrFI CCNGG 2 cut(s) 201, 650
SduI GDGCHC 4 cut(s) 350, 739, 1421, 1625
SfaNI GCATC 3 cut(s) 323, 621, 1037
SinI GGWCC 3 cut(s) 595, 1071, 1118
SmiMI CAYNNNNRTG 2 cut(s) 809, 878
SmlI CTYRAG 2 cut(s) 278, 1558
SmoI CTYRAG 2 cut(s) 278, 1558
SsiI CCGC 3 cut(s) 254, 1038, 1170
SspMI CTAG 2 cut(s) 89, 620
SstI GAGCTC 1 cut(s) 350
StyD4I CCNGG 2 cut(s) 199, 648
StyI CCWWGG 7 cut(s) 88, 354, 501, 713, 741, 1067, 1287
TaaI ACNGT 2 cut(s) 753, 1506
TaiI ACGT 1 cut(s) 564
TaqI TCGA 8 cut(s) 98, 166, 183, 572, 956, 1009, 1567, 1630
TaqII GACCGA 1 cut(s) 1500
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 7 cut(s) 8, 95, 365, 484, 670, 1375, 1492
Tru1I TTAA 3 cut(s) 1046, 1401, 1427
Tru9I TTAA 3 cut(s) 1046, 1401, 1427
TscAI CASTG 4 cut(s) 61, 528, 1395, 1426
TseFI GTSAC 6 cut(s) 71, 283, 1354, 1500, 1512, 1600
TseI GCWGC 4 cut(s) 266, 269, 1306, 1309
Tsp45I GTSAC 6 cut(s) 71, 283, 1354, 1500, 1512, 1600
TspDTI ATGAA 5 cut(s) 470, 716, 764, 816, 924
TspGWI ACGGA 2 cut(s) 348, 525
TspRI CASTG 4 cut(s) 61, 528, 1395, 1426
VpaK11BI GGWCC 3 cut(s) 595, 1071, 1118
VspI ATTAAT 1 cut(s) 1046
XagI CCTNNNNNAGG 1 cut(s) 109
XapI RAATTY 5 cut(s) 424, 541, 952, 1548, 1669
XceI RCATGY 2 cut(s) 877, 1353
XcmI CCANNNNNNNNNTGG 2 cut(s) 584, 1549
XmaJI CCTAGG 1 cut(s) 88
XmiI GTMKAC 2 cut(s) 441, 1062
XspI CTAG 2 cut(s) 89, 620
Zsp2I ATGCAT 1 cut(s) 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.