Prupe.1G238100_v2.0.a1

TPX2 (targeting protein for Xklp2) protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001269
Physical Location & Seq
Forward (+)
25283557 .. 25287835
4279 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G238100.1

Sequence Viewer

Length: 1629 bp
ATGGGTGAATCAGCCTGTCTGATGAGATCGTTCTCTAGCCCTTCTAAGTGTTCCAGTGAAGTCAAAGAGGGGGACCCCCTTCGTTGCCTAGGAGAATCAATCTCCTTCGGCAGGTATATGTCAGAACCCCTTGCTTGGGAGAAATGGTCAGCATTTTCCCACAATCGCTACTTAGAAGAAGTCGAAAAGTTTTCCAAACCCGGCTCTGTTGCTGAGAAGAAGGCTTATTTTGAAGCTCACTACAAGAGAAAAGCTGCTGAGAAAGCAGCAGCCTTGCTTGAGGTAACAAATGCAAGTGCCAGTAATGTCTCAGAGTCAGTAAATATGTACAAAAATTGTGACAGCTTCTCCAACATAGAGTCAGCGAATGGAGAGAGCCATATGGTTGTCGATAAACAACAGGAAAATTTTGTAACAAATTCGGAGGTAGTAGTATGCCCAGCTGATATGAGTGGCCCTAATCCAAATGTTGAAGGGAATCAATTGGATGTTTCCATGGTGGACGGAGCAGAAGCAGTGGTTCAAGAAAGCGTTAATTTGGCGAATCCTATTCAGGTTGAAATCTCAAACAAGTTTGAAAATGACAAAGACCAAGATGAGATTGTTGCCACCCAAGAAGAGAAGATTCCAAATAAGGAAGCAGCTGGTGAGGAGAATCTAGCTTCAACGAACAAGAAAAGATTGATAAACTCTTCCCCAAGGTTATCCACTAAAGGTAGAGCATCCAAGGCCCCAATGTCTCCTGCCAAACAAGCAACTCGTGTGCAAACCATAAATGGGAAAAATGTCACTCAAAAGGGTAAGAAATTTTCGTCAGACTTAGTTGACAAGAGGAGATTGACTGGAAAATCTCTCCACATGTCAATCCATTTCAGTTCTCGTGCTGGTGAAAGTGAAACCAGTAAAATAACTTCACCAGTTGTGGAGAAGACCAAAAATTCAAGAAGTAACACTACTATGTTTAATATCTCCATGAATAAGCCGGCTTCTCGACAAAGTACGGCTAGGGCATCTGTTAATGGGGTATTAAAGCAGTCTTCAGCAGACCTATGGTCCATAGATAGAAGGGAAAAAACATTACTTAATAAGTCGGTGGCTGGTGCAATTGCAGATGGGATATGGACATCCTCCTCAAAAGAACAATCTTCTACTGCAAATGGAAGTAAACCACGACCCCCTATTGCATCATGTCCTTTTATCTTCAAGAGTCAAGAAAGAGCAGAAAAGCGTAAAGAGTTCTTTCAGAGGCTGGAAGAGAAGAAAAATGCCAAGGAGGCTGAAAAGAAGCAGCTGCAACTGAGATCTAATAAAAAGGAAAAAGCTGGAGGGGATGTTAATAAACTGCGTCAGTGCACTGGCTTAAAAGCCAAACTAAACCAAGATCTGTCTTCAGGATCACAATTTCCAAGTAACCACTTGAACAAGATACCTCTGGCACAGCCTCGGTCACCAAGACTTGGAAGGCAGTCTACTCTCAGCAAGGCCCTGGACCCAAGCTCTAGGCCTTCAGTCAATTCTCACCGCTCCAACTATGCCACAAAAAAATATAAAGTGAGCACAAATAGATCAGTGCCTTCTCTTTTGAAGAAGAATGCTCATGAGAATTCTCCTCCCAATATCCAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

543

Amino Acids

59.64

Weight (kDa)

9.63

Isoelectric Point (pI)

60.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 102
AccB7I CCANNNNNTGG 1 cut(s) 1457
AccBSI CCGCTC 1 cut(s) 1524
AccI GTMKAC 1 cut(s) 1469
AciI CCGC 1 cut(s) 1522
AclWI GGATC 1 cut(s) 1402
AcsI RAATTY 5 cut(s) 406, 418, 806, 937, 1603
AcuI CTGAAG 3 cut(s) 1023, 1374, 1491
AfaI GTAC 2 cut(s) 329, 1000
AfiI CCNNNNNNNGG 5 cut(s) 111, 135, 136, 777, 1457
AflIII ACRYGT 1 cut(s) 858
AhdI GACNNNNNGTC 1 cut(s) 1051
AjnI CCWGG 1 cut(s) 1485
AluBI AGCT 9 cut(s) 236, 254, 345, 443, 644, 662, 1291, 1322, 1497
AluI AGCT 9 cut(s) 236, 254, 345, 443, 644, 662, 1291, 1322, 1497
Alw21I GWGCWC 2 cut(s) 1355, 1559
Alw26I GTCTC 2 cut(s) 313, 744
Alw44I GTGCAC 1 cut(s) 1351
AlwI GGATC 1 cut(s) 1402
AlwNI CAGNNNCTG 1 cut(s) 1249
AoxI GGCC 4 cut(s) 454, 729, 1482, 1502
ApaLI GTGCAC 1 cut(s) 1351
ApeKI GCWGC 6 cut(s) 254, 266, 269, 641, 1288, 1291
ApoI RAATTY 5 cut(s) 406, 418, 806, 937, 1603
AspA2I CCTAGG 1 cut(s) 88
AspS9I GGNCC 6 cut(s) 73, 455, 730, 1053, 1483, 1489
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 6 cut(s) 17, 659, 899, 906, 1440, 1511
AvaII GGWCC 3 cut(s) 73, 1053, 1489
AvrII CCTAGG 1 cut(s) 88
BaeGI GKGCMC 1 cut(s) 1355
BarI GAAGNNNNNNTAC 6 cut(s) 895, 927, 937, 969, 1453, 1485
BauI CACGAG 2 cut(s) 759, 879
BbsI GAAGAC 3 cut(s) 935, 1029, 1380
Bbv12I GWGCWC 2 cut(s) 1355, 1559
BbvI GCAGC 6 cut(s) 241, 278, 281, 653, 1278, 1300
BccI CCATC 1 cut(s) 1106
BceAI ACGGC 1 cut(s) 1017
BciT130I CCWGG 1 cut(s) 1487
BcnI CCSGG 1 cut(s) 201
BcoDI GTCTC 2 cut(s) 313, 744
BfaI CTAG 5 cut(s) 36, 89, 659, 1005, 1500
BfuAI ACCTGC 1 cut(s) 102
BglI GCCNNNNNGGC 1 cut(s) 1274
BglII AGATCT 2 cut(s) 1301, 1381
BisI GCNGC 6 cut(s) 255, 267, 270, 642, 1289, 1292
BlnI CCTAGG 1 cut(s) 88
BlsI GCNGC 6 cut(s) 256, 268, 271, 643, 1290, 1293
Bme1390I CCNGG 2 cut(s) 201, 1487
Bme18I GGWCC 3 cut(s) 73, 1053, 1489
BmeRI GACNNNNNGTC 1 cut(s) 1051
BmgT120I GGNCC 6 cut(s) 73, 455, 730, 1053, 1483, 1489
BmiI GGNNCC 4 cut(s) 74, 75, 732, 1491
BmrFI CCNGG 2 cut(s) 201, 1487
BmsI GCATC 3 cut(s) 731, 1019, 1193
BpiI GAAGAC 3 cut(s) 935, 1029, 1380
BpmI CTGGAG 1 cut(s) 1344
BpuEI CTTGAG 1 cut(s) 299
BpuMI CCSGG 1 cut(s) 201
BsaJI CCNNGG 7 cut(s) 88, 495, 698, 726, 1269, 1442, 1485
BsaXI ACNNNNNCTCC 6 cut(s) 131, 161, 332, 362, 1317, 1347
Bsc4I CCNNNNNNNGG 5 cut(s) 111, 135, 136, 777, 1457
Bse118I RCCGGY 1 cut(s) 982
Bse1I ACTGG 6 cut(s) 54, 300, 847, 900, 917, 1360
BseBI CCWGG 1 cut(s) 1487
BseDI CCNNGG 7 cut(s) 88, 495, 698, 726, 1269, 1442, 1485
BseGI GGATG 4 cut(s) 493, 722, 1124, 1336
BseLI CCNNNNNNNGG 5 cut(s) 111, 135, 136, 777, 1457
BseMII CTCAG 5 cut(s) 204, 249, 324, 1289, 1489
BseNI ACTGG 6 cut(s) 54, 300, 847, 900, 917, 1360
BseRI GAGGAG 4 cut(s) 665, 847, 1120, 1599
BseSI GKGCMC 1 cut(s) 1355
BseXI GCAGC 6 cut(s) 241, 278, 281, 653, 1278, 1300
BseYI CCCAGC 1 cut(s) 439
BshFI GGCC 4 cut(s) 456, 731, 1484, 1504
BsiHKAI GWGCWC 2 cut(s) 1355, 1559
BsiSI CCGG 2 cut(s) 201, 983
BslFI GGGAC 1 cut(s) 86
BslI CCNNNNNNNGG 5 cut(s) 111, 135, 136, 777, 1457
BsmAI GTCTC 2 cut(s) 313, 744
BsmFI GGGAC 1 cut(s) 86
BsmI GAATGC 1 cut(s) 1597
BsnI GGCC 4 cut(s) 456, 731, 1484, 1504
Bsp1286I GDGCHC 2 cut(s) 1355, 1559
Bsp1407I TGTACA 1 cut(s) 327
Bsp143I GATC 5 cut(s) 26, 1301, 1381, 1394, 1565
Bsp19I CCATGG 1 cut(s) 495
BspACI CCGC 1 cut(s) 1522
BspANI GGCC 4 cut(s) 456, 731, 1484, 1504
BspCNI CTCAG 5 cut(s) 205, 250, 323, 1290, 1488
BspHI TCATGA 1 cut(s) 1597
BspLI GGNNCC 4 cut(s) 74, 75, 732, 1491
BspMI ACCTGC 1 cut(s) 102
BspPI GGATC 1 cut(s) 1402
BsrBI CCGCTC 1 cut(s) 1524
BsrFI RCCGGY 1 cut(s) 982
BsrGI TGTACA 1 cut(s) 327
BsrI ACTGG 6 cut(s) 54, 300, 847, 900, 917, 1360
BssAI RCCGGY 1 cut(s) 982
BssECI CCNNGG 7 cut(s) 88, 495, 698, 726, 1269, 1442, 1485
BssMI GATC 5 cut(s) 26, 1301, 1381, 1394, 1565
BssSI CACGAG 2 cut(s) 759, 879
BssT1I CCWWGG 5 cut(s) 88, 495, 698, 726, 1269
Bst2BI CACGAG 2 cut(s) 759, 879
Bst2UI CCWGG 1 cut(s) 1487
Bst6I CTCTTC 3 cut(s) 612, 697, 1248
BstAUI TGTACA 1 cut(s) 327
BstC8I GCNNGC 1 cut(s) 984
BstDEI CTNAG 8 cut(s) 45, 172, 213, 258, 310, 820, 1298, 1475
BstDSI CCRYGG 1 cut(s) 495
BstEII GGTNACC 1 cut(s) 1446
BstENI CCTNNNNNAGG 1 cut(s) 109
BstF5I GGATG 4 cut(s) 493, 722, 1124, 1336
BstKTI GATC 5 cut(s) 29, 1304, 1384, 1397, 1568
BstMAI GTCTC 2 cut(s) 313, 744
BstMBI GATC 5 cut(s) 26, 1301, 1381, 1394, 1565
BstMWI GCNNNNNNNGC 4 cut(s) 263, 728, 752, 1274
BstNI CCWGG 1 cut(s) 1487
BstNSI RCATGY 1 cut(s) 862
BstPI GGTNACC 1 cut(s) 1446
BstSCI CCNGG 2 cut(s) 199, 1485
BstSLI GKGCMC 1 cut(s) 1355
BstV1I GCAGC 6 cut(s) 241, 278, 281, 653, 1278, 1300
BstV2I GAAGAC 3 cut(s) 935, 1029, 1380
BstX2I RGATCY 2 cut(s) 1301, 1381
BstYI RGATCY 2 cut(s) 1301, 1381
BsuRI GGCC 4 cut(s) 456, 731, 1484, 1504
BtgI CCRYGG 1 cut(s) 495
BtsCI GGATG 4 cut(s) 493, 722, 1124, 1336
BtsI GCAGTG 1 cut(s) 522
BtsIMutI CAGTG 5 cut(s) 61, 522, 1353, 1355, 1575
BveI ACCTGC 1 cut(s) 102
Cac8I GCNNGC 1 cut(s) 984
CaiI CAGNNNCTG 1 cut(s) 1249
CciI TCATGA 1 cut(s) 1597
Cfr10I RCCGGY 1 cut(s) 982
Cfr13I GGNCC 6 cut(s) 73, 455, 730, 1053, 1483, 1489
CseI GACGC 1 cut(s) 1334
Csp6I GTAC 2 cut(s) 328, 999
CviAII CATG 5 cut(s) 496, 859, 973, 1188, 1598
CviQI GTAC 2 cut(s) 328, 999
DdeI CTNAG 8 cut(s) 45, 172, 213, 258, 310, 820, 1298, 1475
DpnI GATC 5 cut(s) 28, 1303, 1383, 1396, 1567
DpnII GATC 5 cut(s) 26, 1301, 1381, 1394, 1565
DriI GACNNNNNGTC 1 cut(s) 1051
Eam1104I CTCTTC 3 cut(s) 612, 697, 1248
Eam1105I GACNNNNNGTC 1 cut(s) 1051
EarI CTCTTC 3 cut(s) 612, 697, 1248
Eco130I CCWWGG 5 cut(s) 88, 495, 698, 726, 1269
Eco147I AGGCCT 1 cut(s) 1504
Eco47I GGWCC 3 cut(s) 73, 1053, 1489
Eco57I CTGAAG 3 cut(s) 1023, 1374, 1491
Eco91I GGTNACC 1 cut(s) 1446
EcoNI CCTNNNNNAGG 1 cut(s) 109
EcoO109I RGGNCCY 3 cut(s) 73, 730, 1483
EcoO65I GGTNACC 1 cut(s) 1446
EcoRI GAATTC 1 cut(s) 1603
EcoRII CCWGG 1 cut(s) 1485
EcoT14I CCWWGG 5 cut(s) 88, 495, 698, 726, 1269
ErhI CCWWGG 5 cut(s) 88, 495, 698, 726, 1269
FaeI CATG 5 cut(s) 499, 862, 976, 1191, 1601
FaqI GGGAC 1 cut(s) 86
FatI CATG 5 cut(s) 495, 858, 972, 1187, 1597
FauNDI CATATG 1 cut(s) 381
FblI GTMKAC 1 cut(s) 1469
Fnu4HI GCNGC 6 cut(s) 255, 267, 270, 642, 1289, 1292
FokI GGATG 4 cut(s) 500, 709, 1111, 1343
Fsp4HI GCNGC 6 cut(s) 255, 267, 270, 642, 1289, 1292
FspBI CTAG 5 cut(s) 36, 89, 659, 1005, 1500
GluI GCNGC 6 cut(s) 255, 267, 270, 642, 1289, 1292
GsaI CCCAGC 1 cut(s) 443
GsuI CTGGAG 1 cut(s) 1344
HaeIII GGCC 4 cut(s) 456, 731, 1484, 1504
HapII CCGG 2 cut(s) 201, 983
HgaI GACGC 1 cut(s) 1334
Hin1II CATG 5 cut(s) 499, 862, 976, 1191, 1601
HincII GTYRAC 1 cut(s) 826
HindII GTYRAC 1 cut(s) 826
HinfI GANTC 9 cut(s) 8, 95, 314, 359, 478, 544, 625, 655, 1207
HpaII CCGG 2 cut(s) 201, 983
HphI GGTGA 6 cut(s) 17, 659, 899, 906, 1440, 1511
Hpy166II GTNNAC 5 cut(s) 502, 826, 1166, 1353, 1470
Hpy188I TCNGA 6 cut(s) 21, 124, 313, 424, 817, 1245
Hpy188III TCNNGA 8 cut(s) 524, 942, 990, 1204, 1211, 1392, 1598, 1621
Hpy8I GTNNAC 5 cut(s) 502, 826, 1166, 1353, 1470
HpyAV CCTTC 9 cut(s) 51, 89, 115, 214, 467, 1059, 1455, 1515, 1584
HpyCH4V TGCA 8 cut(s) 293, 766, 1103, 1109, 1154, 1184, 1294, 1353
HpyF10VI GCNNNNNNNGC 4 cut(s) 263, 728, 752, 1274
HpyF3I CTNAG 8 cut(s) 45, 172, 213, 258, 310, 820, 1298, 1475
Hsp92II CATG 5 cut(s) 499, 862, 976, 1191, 1601
KflI GGGWCCC 1 cut(s) 73
KroI GCCGGC 1 cut(s) 982
KroNI GCCGGC 1 cut(s) 984
Kzo9I GATC 5 cut(s) 26, 1301, 1381, 1394, 1565
LmnI GCTCC 2 cut(s) 506, 1529
Lsp1109I GCAGC 6 cut(s) 241, 278, 281, 653, 1278, 1300
LweI GCATC 3 cut(s) 731, 1019, 1193
MaeI CTAG 5 cut(s) 36, 89, 659, 1005, 1500
MaeIII GTNAC 7 cut(s) 283, 338, 412, 787, 947, 1409, 1446
MalI GATC 5 cut(s) 28, 1303, 1383, 1396, 1567
MbiI CCGCTC 1 cut(s) 1524
MboI GATC 5 cut(s) 26, 1301, 1381, 1394, 1565
MfeI CAATTG 2 cut(s) 482, 1104
MflI RGATCY 2 cut(s) 1301, 1381
MhlI GDGCHC 2 cut(s) 1355, 1559
MlyI GAGTC 3 cut(s) 323, 368, 1216
MmeI TCCRAC 2 cut(s) 375, 1551
MroNI GCCGGC 1 cut(s) 982
MseI TTAA 7 cut(s) 534, 963, 1017, 1028, 1083, 1335, 1361
MslI CAYNNNNRTG 1 cut(s) 956
MspA1I CMGCKG 3 cut(s) 443, 644, 1291
MspI CCGG 2 cut(s) 201, 983
MspR9I CCNGG 2 cut(s) 201, 1487
MunI CAATTG 2 cut(s) 482, 1104
Mva1269I GAATGC 1 cut(s) 1597
MvaI CCWGG 1 cut(s) 1487
MwoI GCNNNNNNNGC 4 cut(s) 263, 728, 752, 1274
NaeI GCCGGC 1 cut(s) 984
NciI CCSGG 1 cut(s) 201
NcoI CCATGG 1 cut(s) 495
NdeI CATATG 1 cut(s) 381
NdeII GATC 5 cut(s) 26, 1301, 1381, 1394, 1565
NgoMIV GCCGGC 1 cut(s) 982
NlaIII CATG 5 cut(s) 499, 862, 976, 1191, 1601
NlaIV GGNNCC 4 cut(s) 74, 75, 732, 1491
NmuCI GTSAC 3 cut(s) 338, 787, 1446
NspI RCATGY 1 cut(s) 862
PagI TCATGA 1 cut(s) 1597
PceI AGGCCT 1 cut(s) 1504
PciI ACATGT 1 cut(s) 858
PctI GAATGC 1 cut(s) 1597
PdiI GCCGGC 1 cut(s) 984
PfeI GAWTC 6 cut(s) 8, 95, 478, 544, 625, 655
PflMI CCANNNNNTGG 1 cut(s) 1457
PkrI GCNGC 6 cut(s) 256, 268, 271, 643, 1290, 1293
PleI GAGTC 3 cut(s) 322, 367, 1215
PpsI GAGTC 3 cut(s) 322, 367, 1215
PpuMI RGGWCCY 1 cut(s) 73
PscI ACATGT 1 cut(s) 858
Psp5II RGGWCCY 1 cut(s) 73
Psp6I CCWGG 1 cut(s) 1485
PspEI GGTNACC 1 cut(s) 1446
PspFI CCCAGC 1 cut(s) 439
PspGI CCWGG 1 cut(s) 1485
PspN4I GGNNCC 4 cut(s) 74, 75, 732, 1491
PspPI GGNCC 6 cut(s) 73, 455, 730, 1053, 1483, 1489
PspPPI RGGWCCY 1 cut(s) 73
PstNI CAGNNNCTG 1 cut(s) 1249
PsuI RGATCY 2 cut(s) 1301, 1381
PvuII CAGCTG 3 cut(s) 443, 644, 1291
RsaI GTAC 2 cut(s) 329, 1000
RsaNI GTAC 2 cut(s) 328, 999
RseI CAYNNNNRTG 1 cut(s) 956
SaqAI TTAA 7 cut(s) 534, 963, 1017, 1028, 1083, 1335, 1361
SatI GCNGC 6 cut(s) 255, 267, 270, 642, 1289, 1292
Sau3AI GATC 5 cut(s) 26, 1301, 1381, 1394, 1565
Sau96I GGNCC 6 cut(s) 73, 455, 730, 1053, 1483, 1489
SchI GAGTC 3 cut(s) 323, 368, 1216
ScrFI CCNGG 2 cut(s) 201, 1487
SduI GDGCHC 2 cut(s) 1355, 1559
SfaNI GCATC 3 cut(s) 731, 1019, 1193
SinI GGWCC 3 cut(s) 73, 1053, 1489
SmiMI CAYNNNNRTG 1 cut(s) 956
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
SseBI AGGCCT 1 cut(s) 1504
SsiI CCGC 1 cut(s) 1522
SspMI CTAG 5 cut(s) 36, 89, 659, 1005, 1500
StuI AGGCCT 1 cut(s) 1504
StyD4I CCNGG 2 cut(s) 199, 1485
StyI CCWWGG 5 cut(s) 88, 495, 698, 726, 1269
TaqI TCGA 3 cut(s) 183, 390, 991
TaqII GACCGA 1 cut(s) 1434
TatI WGTACW 1 cut(s) 327
TfiI GAWTC 6 cut(s) 8, 95, 478, 544, 625, 655
Tru1I TTAA 7 cut(s) 534, 963, 1017, 1028, 1083, 1335, 1361
Tru9I TTAA 7 cut(s) 534, 963, 1017, 1028, 1083, 1335, 1361
TscAI CASTG 5 cut(s) 61, 522, 1355, 1360, 1575
TseFI GTSAC 3 cut(s) 338, 787, 1446
TseI GCWGC 6 cut(s) 254, 266, 269, 641, 1288, 1291
Tsp45I GTSAC 3 cut(s) 338, 787, 1446
TspDTI ATGAA 1 cut(s) 989
TspGWI ACGGA 1 cut(s) 519
TspRI CASTG 5 cut(s) 61, 522, 1355, 1360, 1575
Van91I CCANNNNNTGG 1 cut(s) 1457
VneI GTGCAC 1 cut(s) 1351
VpaK11BI GGWCC 3 cut(s) 73, 1053, 1489
XagI CCTNNNNNAGG 1 cut(s) 109
XapI RAATTY 5 cut(s) 406, 418, 806, 937, 1603
XceI RCATGY 1 cut(s) 862
XmaJI CCTAGG 1 cut(s) 88
XmiI GTMKAC 1 cut(s) 1469
XspI CTAG 5 cut(s) 36, 89, 659, 1005, 1500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.