RchiOBHm_Chr2g0174031

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001269
Physical Location & Seq
Reverse (-)
86776571 .. 86780438
3868 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54120

Sequence Viewer

Length: 1611 bp
ATGGGAGACTCTACCTGTCTCATGCAGCCCTTCTCTTACGCTTCAGGAATCCCCAATGAGTCAATCGAGGGGAATCCAATTCATGCTCTTGGGCATTCGGTTTCATTTGGGAGGTTTATGACAGATTCATTAGCTTGGGAGAAATGGTCATCATTCTCTCACAATCGATATGTCGAAGAGGCTGAGAGGTATGCTCAACCCGGCTCAGTTGCACAGAAGAAGGCTTTCTTTGAGGCACATTACAAGAAGGTTGCTGCTCAGCGAGCTGCGGCCTTGCTTGAACAAGCAAATGCGGCTGCGAAATCCAACAATGCTGCTCCACCTAAACCCCAAGCCGAGGTCCCGGATCCCCCAAAGTACATGATCTGTGAGCAGGAGCTAAGGGAGAAGGTGAAGGAGCGAAATGGATTTCGTTCGAAAGTTGGAATGGACAAATTCGAAAGCATTGAAATGGTGAAAGTAGCTGAAGAGAATGCTGAAGACGCACAGAGAGAAGAAGAAGCTGTACTTGTGGATACCAGTGGGTATAACTCAAGCATTGAAATGGAGAACTGTGAAAGAGTGCATCCTATTACTGAATACCCAGTTTGGGAGGAGAACTCTGCAGTGGCTGAACTTTCAACCCCGATGCAAGATGCTGATACCGACAAGGTGGAAAATGTCATGGAGCTCGAACACAGTGGTACACTTCAGATGGAAAAGCCTCTGTTAAAGGAGAGTCGCAGCTCTAATCAAGAAGCTTCATTACCAGCAAAAAAGAAGAAAATTCTTTTTTCGTCAAAGTCATCATCAATGTATCACAAAGCACCTAAGCCCCCAACTTCTCCATCCAAACCCACAGCTTCTTGCTTTCCGAGAAGGGAAGGTAATGCCACTCCATTGCAAAAAGCACCCAAGGCCCCAACATCCCCATCCAAACCCACAGCTTCTTGTTTTCCGAGGAGGGAAAGTAATGCCACTCCATTACTCAAAGCACCCAAACCCCCAACATCTCCAGCCAAACCCACAGCTTCTTGTTTTCCAAGAAGGGAAGGTAATGCCACTCCATTACATAAAAAGCTGTCATCAGCTTCTGAGGAGAAGAAAAGATCTACACCAAAATCTCTTCAAAAGTCGGTCAGTTTTACACCCATCAGAGAGCTCTCTAGGTTTACTTCAACAGTCATGAGAAAGATTGAGAACTCAAGAGTTGGTACAAGTTCTTTCAAGACACCTAAAGATTGCCTAACTCTTCCAAAAACTCCAACCACGGTATCTAAGAATGAGGTGCACAAGCATCCTTCAATAACTCCTTGTTCAGAAAAGAGAAGGGCAAAAACGCCAGGCTATCCGTCATCAGTCACTGGAGCCAAAATGTTTGGGCCCAAATGGCGCTCAATCCGTACAGAAAACAAAGCGCAATCCCCATTTTCATCTGCCTCTTTCAACTTAAGAACTGAAGAACGAGCTGCAAGTAGAAAGAAGGCAAGCCTTTGCTTTTGTTCTTTTCTAGATATATGTTCCCGAACTATAATCAAATCTATTATGTACGAGCTACTGTATATGTGTAAAGTACTTATGGATGTCAATACTTCATTGCTTTCAGAAGCTCGAAGAAAAGTTCAATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

536

Amino Acids

59.55

Weight (kDa)

9.4

Isoelectric Point (pI)

56.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 269, 293
AclWI GGATC 2 cut(s) 341, 354
AcsI RAATTY 2 cut(s) 434, 765
AcuI CTGAAG 5 cut(s) 27, 486, 498, 674, 1458
AfaI GTAC 7 cut(s) 359, 507, 685, 1195, 1384, 1529, 1554
AfiI CCNNNNNNNGG 2 cut(s) 337, 589
AflII CTTAAG 1 cut(s) 1429
AjnI CCWGG 1 cut(s) 1321
Alw21I GWGCWC 3 cut(s) 672, 1143, 1272
Alw26I GTCTC 1 cut(s) 23
Alw44I GTGCAC 1 cut(s) 1268
AlwI GGATC 2 cut(s) 341, 354
AlwNI CAGNNNCTG 3 cut(s) 611, 1073, 1343
AoxI GGCC 3 cut(s) 270, 897, 1361
ApaI GGGCCC 1 cut(s) 1365
ApaLI GTGCAC 1 cut(s) 1268
ApeKI GCWGC 7 cut(s) 25, 254, 266, 296, 314, 723, 1448
ApoI RAATTY 2 cut(s) 434, 765
Asp700I GAANNNNTTC 1 cut(s) 224
AspLEI GCGC 2 cut(s) 1374, 1399
AspS9I GGNCC 4 cut(s) 340, 898, 1361, 1362
AsuC2I CCSGG 2 cut(s) 201, 344
AsuHPI GGTGA 2 cut(s) 403, 466
AsuII TTCGAA 2 cut(s) 416, 438
AvaII GGWCC 1 cut(s) 340
BaeGI GKGCMC 2 cut(s) 1272, 1365
BaeI ACNNNNGTAYC 2 cut(s) 1236, 1269
BamHI GGATCC 1 cut(s) 346
BanII GRGCYC 3 cut(s) 672, 1143, 1365
BarI GAAGNNNNNNTAC 4 cut(s) 489, 521, 729, 761
BbsI GAAGAC 1 cut(s) 486
Bbv12I GWGCWC 3 cut(s) 672, 1143, 1272
BbvI GCAGC 7 cut(s) 37, 241, 253, 283, 301, 735, 1435
BccI CCATC 4 cut(s) 688, 835, 919, 1139
BciT130I CCWGG 1 cut(s) 1323
BciVI GTATCC 1 cut(s) 508
BcnI CCSGG 2 cut(s) 201, 344
BcoDI GTCTC 1 cut(s) 23
BfaI CTAG 2 cut(s) 1146, 1490
BfmI CTRYAG 1 cut(s) 603
BfoI RGCGCY 1 cut(s) 1375
BfrI CTTAAG 1 cut(s) 1429
BfuI GTATCC 1 cut(s) 508
BglI GCCNNNNNGGC 1 cut(s) 1369
BglII AGATCT 1 cut(s) 1088
BisI GCNGC 9 cut(s) 26, 255, 267, 270, 294, 297, 315, 724, 1449
BlpI GCTNAGC 1 cut(s) 258
BlsI GCNGC 9 cut(s) 27, 256, 268, 271, 295, 298, 316, 725, 1450
BmcAI AGTACT 1 cut(s) 1554
Bme1390I CCNGG 3 cut(s) 201, 344, 1323
Bme18I GGWCC 1 cut(s) 340
BmgT120I GGNCC 4 cut(s) 340, 898, 1361, 1362
BmiI GGNNCC 5 cut(s) 342, 348, 900, 1348, 1363
BmrFI CCNGG 3 cut(s) 201, 344, 1323
BmrI ACTGGG 1 cut(s) 578
BmsI GCATC 4 cut(s) 574, 618, 625, 1285
BmuI ACTGGG 1 cut(s) 578
BpiI GAAGAC 1 cut(s) 486
BplI GAGNNNNNCTC 4 cut(s) 178, 210, 584, 616
BpmI CTGGAG 2 cut(s) 978, 1365
Bpu10I CCTNAGC 2 cut(s) 380, 810
Bpu1102I GCTNAGC 1 cut(s) 258
Bpu14I TTCGAA 2 cut(s) 416, 438
BpuEI CTTGAG 2 cut(s) 517, 1168
BpuMI CCSGG 2 cut(s) 201, 344
Bsa29I ATCGAT 1 cut(s) 166
BsaJI CCNNGG 4 cut(s) 336, 894, 938, 1248
BsaXI ACNNNNNCTCC 4 cut(s) 131, 161, 934, 964
Bsc4I CCNNNNNNNGG 2 cut(s) 337, 589
Bse1I ACTGG 3 cut(s) 519, 584, 1348
Bse3DI GCAATG 2 cut(s) 878, 1574
BseBI CCWGG 1 cut(s) 1323
BseCI ATCGAT 1 cut(s) 166
BseDI CCNNGG 4 cut(s) 336, 894, 938, 1248
BseGI GGATG 6 cut(s) 565, 827, 905, 911, 1276, 1567
BseLI CCNNNNNNNGG 2 cut(s) 337, 589
BseMI GCAATG 2 cut(s) 878, 1574
BseMII CTCAG 4 cut(s) 174, 219, 272, 1065
BseNI ACTGG 3 cut(s) 519, 584, 1348
BseRI GAGGAG 3 cut(s) 608, 955, 1091
BseSI GKGCMC 2 cut(s) 1272, 1365
BseXI GCAGC 7 cut(s) 37, 241, 253, 283, 301, 735, 1435
BshFI GGCC 3 cut(s) 272, 899, 1363
BshVI ATCGAT 1 cut(s) 166
BsiHKAI GWGCWC 3 cut(s) 672, 1143, 1272
BsiSI CCGG 2 cut(s) 201, 344
BslFI GGGAC 1 cut(s) 326
BslI CCNNNNNNNGG 2 cut(s) 337, 589
BsmAI GTCTC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 326
BsmI GAATGC 2 cut(s) 94, 478
BsnI GGCC 3 cut(s) 272, 899, 1363
Bsp119I TTCGAA 2 cut(s) 416, 438
Bsp120I GGGCCC 1 cut(s) 1361
Bsp1286I GDGCHC 4 cut(s) 672, 1143, 1272, 1365
Bsp143I GATC 3 cut(s) 346, 363, 1088
Bsp1720I GCTNAGC 1 cut(s) 258
BspACI CCGC 2 cut(s) 269, 293
BspANI GGCC 3 cut(s) 272, 899, 1363
BspCNI CTCAG 4 cut(s) 175, 218, 271, 1066
BspDI ATCGAT 1 cut(s) 166
BspHI TCATGA 1 cut(s) 1164
BspLI GGNNCC 5 cut(s) 342, 348, 900, 1348, 1363
BspMAI CTGCAG 1 cut(s) 607
BspPI GGATC 2 cut(s) 341, 354
BspT104I TTCGAA 2 cut(s) 416, 438
BspTI CTTAAG 1 cut(s) 1429
BsrDI GCAATG 2 cut(s) 878, 1574
BsrI ACTGG 3 cut(s) 519, 584, 1348
BssECI CCNNGG 4 cut(s) 336, 894, 938, 1248
BssMI GATC 3 cut(s) 346, 363, 1088
BssT1I CCWWGG 1 cut(s) 894
Bst2UI CCWGG 1 cut(s) 1323
Bst4CI ACNGT 5 cut(s) 554, 680, 1162, 1252, 1539
Bst6I CTCTTC 4 cut(s) 171, 462, 1110, 1236
BstAFI CTTAAG 1 cut(s) 1429
BstBI TTCGAA 2 cut(s) 416, 438
BstC8I GCNNGC 2 cut(s) 264, 1468
BstDEI CTNAG 7 cut(s) 183, 205, 258, 380, 810, 1074, 1257
BstDSI CCRYGG 1 cut(s) 1248
BstF5I GGATG 6 cut(s) 565, 827, 905, 911, 1276, 1567
BstH2I RGCGCY 1 cut(s) 1375
BstHHI GCGC 2 cut(s) 1374, 1399
BstKTI GATC 3 cut(s) 349, 366, 1091
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 3 cut(s) 346, 363, 1088
BstMWI GCNNNNNNNGC 5 cut(s) 263, 293, 482, 896, 1369
BstNI CCWGG 1 cut(s) 1323
BstSCI CCNGG 3 cut(s) 199, 342, 1321
BstSFI CTRYAG 1 cut(s) 603
BstSLI GKGCMC 2 cut(s) 1272, 1365
BstV1I GCAGC 7 cut(s) 37, 241, 253, 283, 301, 735, 1435
BstV2I GAAGAC 1 cut(s) 486
BstX2I RGATCY 2 cut(s) 346, 1088
BstYI RGATCY 2 cut(s) 346, 1088
Bsu15I ATCGAT 1 cut(s) 166
BsuI GTATCC 1 cut(s) 508
BsuRI GGCC 3 cut(s) 272, 899, 1363
BsuTUI ATCGAT 1 cut(s) 166
BtgI CCRYGG 1 cut(s) 1248
BtsCI GGATG 6 cut(s) 565, 827, 905, 911, 1276, 1567
BtsI GCAGTG 1 cut(s) 612
BtsIMutI CAGTG 4 cut(s) 526, 612, 685, 1341
Cac8I GCNNGC 2 cut(s) 264, 1468
CaiI CAGNNNCTG 3 cut(s) 611, 1073, 1343
CciI TCATGA 1 cut(s) 1164
CfoI GCGC 2 cut(s) 1374, 1399
Cfr13I GGNCC 4 cut(s) 340, 898, 1361, 1362
ClaI ATCGAT 1 cut(s) 166
CseI GACGC 1 cut(s) 491
Csp6I GTAC 7 cut(s) 358, 506, 684, 1194, 1383, 1528, 1553
CviAII CATG 5 cut(s) 22, 83, 361, 664, 1165
CviQI GTAC 7 cut(s) 358, 506, 684, 1194, 1383, 1528, 1553
DdeI CTNAG 7 cut(s) 183, 205, 258, 380, 810, 1074, 1257
DpnI GATC 3 cut(s) 348, 365, 1090
DpnII GATC 3 cut(s) 346, 363, 1088
Eam1104I CTCTTC 4 cut(s) 171, 462, 1110, 1236
EarI CTCTTC 4 cut(s) 171, 462, 1110, 1236
Ecl136II GAGCTC 2 cut(s) 670, 1141
Eco130I CCWWGG 1 cut(s) 894
Eco24I GRGCYC 3 cut(s) 672, 1143, 1365
Eco47I GGWCC 1 cut(s) 340
Eco53kI GAGCTC 2 cut(s) 670, 1141
Eco57I CTGAAG 5 cut(s) 27, 486, 498, 674, 1458
EcoICRI GAGCTC 2 cut(s) 670, 1141
EcoO109I RGGNCCY 2 cut(s) 340, 898
EcoRII CCWGG 1 cut(s) 1321
EcoT14I CCWWGG 1 cut(s) 894
EcoT38I GRGCYC 3 cut(s) 672, 1143, 1365
ErhI CCWWGG 1 cut(s) 894
FaeI CATG 5 cut(s) 25, 86, 364, 667, 1168
FalI AAGNNNNNCTT 4 cut(s) 212, 244, 492, 524
FaqI GGGAC 1 cut(s) 326
FatI CATG 5 cut(s) 21, 82, 360, 663, 1164
Fnu4HI GCNGC 9 cut(s) 26, 255, 267, 270, 294, 297, 315, 724, 1449
FokI GGATG 6 cut(s) 552, 814, 892, 898, 1263, 1574
FriOI GRGCYC 3 cut(s) 672, 1143, 1365
Fsp4HI GCNGC 9 cut(s) 26, 255, 267, 270, 294, 297, 315, 724, 1449
FspBI CTAG 2 cut(s) 1146, 1490
GlaI GCGC 2 cut(s) 1373, 1398
GluI GCNGC 9 cut(s) 26, 255, 267, 270, 294, 297, 315, 724, 1449
GsuI CTGGAG 2 cut(s) 978, 1365
HaeII RGCGCY 1 cut(s) 1375
HaeIII GGCC 3 cut(s) 272, 899, 1363
HapII CCGG 2 cut(s) 201, 344
HgaI GACGC 1 cut(s) 491
HhaI GCGC 2 cut(s) 1374, 1399
Hin1II CATG 5 cut(s) 25, 86, 364, 667, 1168
Hin6I GCGC 2 cut(s) 1372, 1397
HinP1I GCGC 2 cut(s) 1372, 1397
HindIII AAGCTT 1 cut(s) 738
HinfI GANTC 6 cut(s) 8, 48, 59, 73, 125, 718
HpaII CCGG 2 cut(s) 201, 344
HphI GGTGA 2 cut(s) 403, 466
Hpy166II GTNNAC 3 cut(s) 686, 1152, 1270
Hpy188I TCNGA 7 cut(s) 693, 855, 939, 1075, 1136, 1300, 1585
Hpy188III TCNNGA 7 cut(s) 45, 734, 1165, 1185, 1207, 1490, 1503
Hpy8I GTNNAC 3 cut(s) 686, 1152, 1270
HpyCH4III ACNGT 5 cut(s) 554, 680, 1162, 1252, 1539
HpyCH4V TGCA 8 cut(s) 25, 212, 565, 605, 631, 883, 1270, 1451
HpyF10VI GCNNNNNNNGC 5 cut(s) 263, 293, 482, 896, 1369
HpyF3I CTNAG 7 cut(s) 183, 205, 258, 380, 810, 1074, 1257
Hsp92II CATG 5 cut(s) 25, 86, 364, 667, 1168
HspAI GCGC 2 cut(s) 1372, 1397
Kzo9I GATC 3 cut(s) 346, 363, 1088
LmnI GCTCC 5 cut(s) 322, 376, 397, 667, 1346
Lsp1109I GCAGC 7 cut(s) 37, 241, 253, 283, 301, 735, 1435
LweI GCATC 4 cut(s) 574, 618, 625, 1285
MaeI CTAG 2 cut(s) 1146, 1490
MaeIII GTNAC 1 cut(s) 1339
MalI GATC 3 cut(s) 348, 365, 1090
MboI GATC 3 cut(s) 346, 363, 1088
MflI RGATCY 2 cut(s) 346, 1088
MhlI GDGCHC 4 cut(s) 672, 1143, 1272, 1365
MluCI AATT 3 cut(s) 78, 434, 765
MlyI GAGTC 3 cut(s) 2, 68, 727
MmeI TCCRAC 3 cut(s) 330, 403, 1268
MroXI GAANNNNTTC 1 cut(s) 224
MseI TTAA 2 cut(s) 710, 1430
MslI CAYNNNNRTG 2 cut(s) 449, 542
MspCI CTTAAG 1 cut(s) 1429
MspI CCGG 2 cut(s) 201, 344
MspR9I CCNGG 3 cut(s) 201, 344, 1323
Mva1269I GAATGC 2 cut(s) 94, 478
MvaI CCWGG 1 cut(s) 1323
MwoI GCNNNNNNNGC 5 cut(s) 263, 293, 482, 896, 1369
NciI CCSGG 2 cut(s) 201, 344
NdeII GATC 3 cut(s) 346, 363, 1088
NlaIII CATG 5 cut(s) 25, 86, 364, 667, 1168
NlaIV GGNNCC 5 cut(s) 342, 348, 900, 1348, 1363
NmeAIII GCCGAG 1 cut(s) 361
NmuCI GTSAC 1 cut(s) 1339
NspV TTCGAA 2 cut(s) 416, 438
PagI TCATGA 1 cut(s) 1164
PctI GAATGC 2 cut(s) 94, 478
PdmI GAANNNNTTC 1 cut(s) 224
PfeI GAWTC 3 cut(s) 48, 73, 125
PfoI TCCNGGA 1 cut(s) 342
PkrI GCNGC 9 cut(s) 27, 256, 268, 271, 295, 298, 316, 725, 1450
PleI GAGTC 3 cut(s) 2, 67, 726
PpsI GAGTC 3 cut(s) 2, 67, 726
PpuMI RGGWCCY 1 cut(s) 340
Psp124BI GAGCTC 2 cut(s) 672, 1143
Psp5II RGGWCCY 1 cut(s) 340
Psp6I CCWGG 1 cut(s) 1321
PspGI CCWGG 1 cut(s) 1321
PspN4I GGNNCC 5 cut(s) 342, 348, 900, 1348, 1363
PspOMI GGGCCC 1 cut(s) 1361
PspPI GGNCC 4 cut(s) 340, 898, 1361, 1362
PspPPI RGGWCCY 1 cut(s) 340
PstI CTGCAG 1 cut(s) 607
PstNI CAGNNNCTG 3 cut(s) 611, 1073, 1343
PsuI RGATCY 2 cut(s) 346, 1088
RsaI GTAC 7 cut(s) 359, 507, 685, 1195, 1384, 1529, 1554
RsaNI GTAC 7 cut(s) 358, 506, 684, 1194, 1383, 1528, 1553
RseI CAYNNNNRTG 2 cut(s) 449, 542
SacI GAGCTC 2 cut(s) 672, 1143
SaqAI TTAA 2 cut(s) 710, 1430
SatI GCNGC 9 cut(s) 26, 255, 267, 270, 294, 297, 315, 724, 1449
Sau3AI GATC 3 cut(s) 346, 363, 1088
Sau96I GGNCC 4 cut(s) 340, 898, 1361, 1362
ScaI AGTACT 1 cut(s) 1554
SchI GAGTC 3 cut(s) 2, 68, 727
ScrFI CCNGG 3 cut(s) 201, 344, 1323
SduI GDGCHC 4 cut(s) 672, 1143, 1272, 1365
SfaNI GCATC 4 cut(s) 574, 618, 625, 1285
SfcI CTRYAG 1 cut(s) 603
SfuI TTCGAA 2 cut(s) 416, 438
SinI GGWCC 1 cut(s) 340
SmiMI CAYNNNNRTG 2 cut(s) 449, 542
SmlI CTYRAG 3 cut(s) 532, 1183, 1429
SmoI CTYRAG 3 cut(s) 532, 1183, 1429
Sse9I AATT 3 cut(s) 78, 434, 765
SsiI CCGC 2 cut(s) 269, 293
SspMI CTAG 2 cut(s) 1146, 1490
SstI GAGCTC 2 cut(s) 672, 1143
StyD4I CCNGG 3 cut(s) 199, 342, 1321
StyI CCWWGG 1 cut(s) 894
TaaI ACNGT 5 cut(s) 554, 680, 1162, 1252, 1539
TaqI TCGA 7 cut(s) 66, 166, 174, 416, 438, 672, 1591
TaqII GACCGA 1 cut(s) 1105
TasI AATT 3 cut(s) 78, 434, 765
TatI WGTACW 3 cut(s) 357, 505, 1552
TauI GCSGC 2 cut(s) 272, 296
TfiI GAWTC 3 cut(s) 48, 73, 125
Tru1I TTAA 2 cut(s) 710, 1430
Tru9I TTAA 2 cut(s) 710, 1430
TscAI CASTG 4 cut(s) 526, 612, 685, 1348
TseFI GTSAC 1 cut(s) 1339
TseI GCWGC 7 cut(s) 25, 254, 266, 296, 314, 723, 1448
Tsp45I GTSAC 1 cut(s) 1339
TspDTI ATGAA 6 cut(s) 71, 93, 117, 732, 1401, 1563
TspGWI ACGGA 2 cut(s) 1320, 1370
TspRI CASTG 4 cut(s) 526, 612, 685, 1348
Vha464I CTTAAG 1 cut(s) 1429
VneI GTGCAC 1 cut(s) 1268
VpaK11BI GGWCC 1 cut(s) 340
XapI RAATTY 2 cut(s) 434, 765
XbaI TCTAGA 1 cut(s) 1489
XmnI GAANNNNTTC 1 cut(s) 224
XspI CTAG 2 cut(s) 1146, 1490
ZrmI AGTACT 1 cut(s) 1554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.