Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001269

Physical Location & Seq

Genomic Coordinates Forward (+)

1147568 .. 1150598

Gene Structure 3031 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1018500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1632 bp

ATGGGAGAATCGACGTGTCTGATGCGGCCGTTCTCCTATGCTTCCGGCATCCCCAACGACACATACGAGGGGAACCCGATTCATGCTCTCGGGCAGTCAGTGTCGTTCGGGAGGTTCGTGTCGGAGTCCGTAGCTTGGGATAAATGGTCGACAATCTCGCACAACCGGCATGTGGAGGAGGCCGAGAGGTACTCCCGCCCGGGTTCAGTTGCGCAGAAAAAGGCTTTCTTTGAAGCACATTACAAGAAAATCGCTGCTCAAAGAGCCGCTGCGGTGCTTGAACAAGCCAGTGCCAATGCCAATGCTGCTGCTGCATTGGAAAATGCCGCCGAAACCAAATCTGAAAGTCGGGATCGAAGGACGAGGATTTCAACCTCTGAAGTGTTCGTCGATGAGCAAAGGGAAGAGAAAGAGGAAGGGAAGGTCTACGAGCCAAATCGAGAAGAAGAATGCTTTGGTAAATATACCAATGACTATAATGCAAACACTGAAATGGAAAAATTTGAAAGCAGCAAGGTGCACCCTGTAGATCATGAAGACCAAGTTTTGGTGGAGAATTCAGCTGAGGTAGAACTTTCGAGCCGAAGTGCTGATAACAACAAGGAGGTAAACGAAATGGAGCTCACCGGCAAACCCCAGATCGAAAAGCCTCTGTTGAAGCAAAGTTACAGCTCGAATCAAGAAGCTTCGACCCCAGTGAAAACGAAAAAAACCAGACCTTTTATTTCCTCCAAATCATCATTAACATATCATAAAGCACCCAAAGTCCCATCTTCTCCAACCAAATCCACAGCTCCTAGTTGTTCCAGAAAGGATGACATTACCACTCCATTGCGTAAATATCCGGCCGCAGAGCTGGAGGACAAGAAGAAATCAACTCCGAAATCAATTCACAAGTCGTTCAGGTTTACACCCATCAGGGAACTTTCTAGGCTGACTTCAACCGTCATCAGGAAGATTGAAAACTCAAGAGCTGGTGCTAGTTCTTCCAAGACATCGAAAGATTGCCCGACTGCTCTGAGGACTCCAACTAAGGTATCTAAGAATGAGGTGCGCCAGCATTCCACCACCACCCCATGTTCAGAAAAGAGAAGGGCCAAAACACCACTTGATCCCTCAGCCAGTGGAACCAAAACTCCTGTGTCCAAATGGCGTTCACTCCGTACAGATTGTTCAAAGATTTTGAGTGCCTGCAGAAACAAGGCGAGGTCCCCATTTTCATCGGCCCCCTTCACCTTAAAAACTGAAGAAAGAGCTGCAAGTAGAAAGAAGAAGCTCGAAGAAAAGTTTAATGCAAATGAGGAACAAAAGGTGCAGGCGCAAAGAAAACTCAAGGAGAAAGAAGAAAAAGAGATTGGAAAGTACCGTCAAACGCTTTGCTTCAAGGCCAGGCCACTTCCTGATTTCTACAAAGACAGAAAATCACCAAAGAAGGAGATACAGAAGGTTCCAGTTACTCCTCAGTCAAAGAATCCAATCACAGTTGAGAGCTCAACGCCTCGACCTCCTAGTGGAGCTTCAATCAAGAGCATTAGCCAAGGAAGGAATAACCGAACCCCAACTCGTTCTCTCATTTCACGATCCATTAAGACTACTCGCGAGAATAAATCTCCAAATATTCAGCTTGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TPX2

Protein Analysis

544

Amino Acids

60.94

Weight (kDa)

9.74

Isoelectric Point (pI)

55.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPX2	PF06886	411 - 478	5.7e-17	Targeting protein for Xklp2 (TPX2) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001269)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01710 AT3G01710 AT3G01710 AT3G01710 AT3G27350 AT3G27350 AT3G27350 AT5G40700 AT5G40700 AT5G40700
fragaria_vesca	FvH4_4g24860 FvH4_4g24860 FvH4_4g24860 FvH4_6g52400 FvH4_6g52400
malus_domestica	MD09G1018500.v1.1 MD13G1108400.v1.1 MD16G1108500.v1.1 MD17G1019000.v1.1
prunus_persica	Prupe.1G238100_v2.0.a1 Prupe.1G238100_v2.0.a1 Prupe.3G298700_v2.0.a1 Prupe.3G298700_v2.0.a1 Prupe.3G298700_v2.0.a1 Prupe.3G298700_v2.0.a1 Prupe.3G298700_v2.0.a1 Prupe.3G298700_v2.0.a1 Prupe.3G298700_v2.0.a1 Prupe.3G298700_v2.0.a1
pyrus_communis	pycom111g01540 pycom13g09370 pycom16g09260 pycom17g01580
rosa_chinensis	RchiOBHm_Chr2g0174031 RchiOBHm_Chr4g0431931
rosa_laevigata	RLG00000006874 RLG00000022238
rosa_multiflora	Rmu_sc0003188.1_g000031 Rmu_sc0004965.1_g000009
rosa_roxburghii	Rroxscaffold_2G00078520 Rroxscaffold_5G00373370
rosa_rugosa	Rorug02G0574000 Rorug02G0574100 Rorug04G0253300 Rorug04G0253300
rosa_samantha	Rh2AG653300 Rh2BG664600 Rh2CG629000 Rh2DG679400 Rh4AG310500 Rh4BG317600 Rh4CG332900 Rh4DG312500
rosa_wichuraiana	Rw2G053660 Rw4G027010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	213
AccB7I	CCANNNNNTGG	1 cut(s)	547
AccI	GTMKAC	2 cut(s)	149, 426
AccII	CGCG	1 cut(s)	1599
AciI	CCGC	6 cut(s)	25, 196, 267, 272, 327, 849
AclWI	GGATC	3 cut(s)	360, 1106, 1575
AcoI	YGGCCR	2 cut(s)	26, 846
AcsI	RAATTY	2 cut(s)	500, 556
AcuI	CTGAAG	2 cut(s)	399, 1266
AfaI	GTAC	3 cut(s)	191, 1165, 1364
AfiI	CCNNNNNNNGG	5 cut(s)	135, 172, 547, 951, 1511
AflIII	ACRYGT	1 cut(s)	14
AjiI	CACGTC	1 cut(s)	15
AjnI	CCWGG	1 cut(s)	1388
AjuI	GAANNNNNNNTTGG	4 cut(s)	438, 470, 1338, 1370
AloI	GAACNNNNNNTCC	2 cut(s)	1120, 1152
Alw21I	GWGCWC	3 cut(s)	522, 624, 1493
Alw44I	GTGCAC	1 cut(s)	518
AlwI	GGATC	3 cut(s)	360, 1106, 1575
Ama87I	CYCGRG	2 cut(s)	89, 199
AoxI	GGCC	7 cut(s)	26, 180, 846, 1095, 1224, 1386, 1391
ApaLI	GTGCAC	1 cut(s)	518
ApeKI	GCWGC	7 cut(s)	254, 269, 305, 308, 311, 510, 1256
ApoI	RAATTY	2 cut(s)	500, 556
AspLEI	GCGC	3 cut(s)	214, 1056, 1321
AspS9I	GGNCC	3 cut(s)	1095, 1209, 1225
AsuC2I	CCSGG	2 cut(s)	200, 201
AsuHPI	GGTGA	3 cut(s)	616, 1225, 1416
AvaI	CYCGRG	2 cut(s)	89, 199
AvaII	GGWCC	1 cut(s)	1209
BaeGI	GKGCMC	1 cut(s)	522
BaeI	ACNNNNGTAYC	2 cut(s)	1020, 1053
BanII	GRGCYC	2 cut(s)	624, 1493
BarI	GAAGNNNNNNTAC	2 cut(s)	650, 682
BbsI	GAAGAC	1 cut(s)	543
Bbv12I	GWGCWC	3 cut(s)	522, 624, 1493
BbvCI	CCTCAGC	2 cut(s)	564, 1117
BbvI	GCAGC	7 cut(s)	241, 256, 292, 295, 298, 522, 1243
BccI	CCATC	2 cut(s)	778, 923
BceAI	ACGGC	1 cut(s)	13
BciT130I	CCWGG	1 cut(s)	1390
BcnI	CCSGG	2 cut(s)	200, 201
BfaI	CTAG	4 cut(s)	798, 930, 981, 1509
BfmI	CTRYAG	2 cut(s)	525, 1192
Bme1390I	CCNGG	3 cut(s)	200, 201, 1390
Bme18I	GGWCC	1 cut(s)	1209
BmeT110I	CYCGRG	2 cut(s)	89, 199
BmgBI	CACGTC	1 cut(s)	15
BmgT120I	GGNCC	3 cut(s)	1095, 1209, 1225
BmiI	GGNNCC	5 cut(s)	74, 1129, 1211, 1227, 1449
BmrFI	CCNGG	3 cut(s)	200, 201, 1390
BmrI	ACTGGG	1 cut(s)	689
BmsI	GCATC	2 cut(s)	12, 57
BmuI	ACTGGG	1 cut(s)	689
BpiI	GAAGAC	1 cut(s)	543
BplI	GAGNNNNNCTC	2 cut(s)	176, 208
BpmI	CTGGAG	1 cut(s)	878
Bpu10I	CCTNAGC	2 cut(s)	564, 1117
BpuEI	CTTGAG	2 cut(s)	952, 1316
BpuMI	CCSGG	2 cut(s)	200, 201
BsaJI	CCNNGG	2 cut(s)	199, 1537
BsaXI	ACNNNNNCTCC	2 cut(s)	1120, 1150
Bsc4I	CCNNNNNNNGG	5 cut(s)	135, 172, 547, 951, 1511
Bse118I	RCCGGY	2 cut(s)	165, 626
Bse1I	ACTGG	4 cut(s)	288, 695, 1122, 1451
Bse3DI	GCAATG	1 cut(s)	830
BseBI	CCWGG	1 cut(s)	1390
BseDI	CCNNGG	2 cut(s)	199, 1537
BseGI	GGATG	2 cut(s)	48, 820
BseLI	CCNNNNNNNGG	5 cut(s)	135, 172, 547, 951, 1511
BseMI	GCAATG	1 cut(s)	830
BseMII	CTCAG	4 cut(s)	555, 1010, 1131, 1475
BseNI	ACTGG	4 cut(s)	288, 695, 1122, 1451
BseRI	GAGGAG	2 cut(s)	191, 1449
BseSI	GKGCMC	1 cut(s)	522
BseX3I	CGGCCG	2 cut(s)	26, 846
BseXI	GCAGC	7 cut(s)	241, 256, 292, 295, 298, 522, 1243
BsgI	GTGCAG	1 cut(s)	1334
Bsh1236I	CGCG	1 cut(s)	1599
Bsh1285I	CGRYCG	2 cut(s)	29, 849
BshFI	GGCC	7 cut(s)	28, 182, 848, 1097, 1226, 1388, 1393
BsiEI	CGRYCG	2 cut(s)	29, 849
BsiHKAI	GWGCWC	3 cut(s)	522, 624, 1493
BsiHKCI	CYCGRG	2 cut(s)	89, 199
BsiSI	CCGG	5 cut(s)	45, 166, 200, 627, 845
BslFI	GGGAC	2 cut(s)	752, 1195
BslI	CCNNNNNNNGG	5 cut(s)	135, 172, 547, 951, 1511
BsmFI	GGGAC	2 cut(s)	752, 1195
BsmI	GAATGC	2 cut(s)	455, 1060
BsnI	GGCC	7 cut(s)	28, 182, 848, 1097, 1226, 1388, 1393
BsoBI	CYCGRG	2 cut(s)	89, 199
Bsp1286I	GDGCHC	3 cut(s)	522, 624, 1493
Bsp143I	GATC	5 cut(s)	352, 529, 639, 1111, 1580
Bsp68I	TCGCGA	1 cut(s)	1599
BspACI	CCGC	6 cut(s)	25, 196, 267, 272, 327, 849
BspANI	GGCC	7 cut(s)	28, 182, 848, 1097, 1226, 1388, 1393
BspCNI	CTCAG	4 cut(s)	556, 1011, 1130, 1474
BspFNI	CGCG	1 cut(s)	1599
BspHI	TCATGA	1 cut(s)	532
BspLI	GGNNCC	5 cut(s)	74, 1129, 1211, 1227, 1449
BspMAI	CTGCAG	1 cut(s)	1196
BspPI	GGATC	3 cut(s)	360, 1106, 1575
BsrDI	GCAATG	1 cut(s)	830
BsrFI	RCCGGY	2 cut(s)	165, 626
BsrI	ACTGG	4 cut(s)	288, 695, 1122, 1451
BssAI	RCCGGY	2 cut(s)	165, 626
BssECI	CCNNGG	2 cut(s)	199, 1537
BssMI	GATC	5 cut(s)	352, 529, 639, 1111, 1580
BssT1I	CCWWGG	1 cut(s)	1537
Bst2UI	CCWGG	1 cut(s)	1390
Bst4CI	ACNGT	3 cut(s)	946, 1367, 1483
Bst6I	CTCTTC	1 cut(s)	399
BstC8I	GCNNGC	3 cut(s)	1058, 1192, 1317
BstDEI	CTNAG	6 cut(s)	564, 1019, 1032, 1041, 1117, 1461
BstF5I	GGATG	2 cut(s)	48, 820
BstFNI	CGCG	1 cut(s)	1599
BstHHI	GCGC	3 cut(s)	214, 1056, 1321
BstKTI	GATC	5 cut(s)	355, 532, 642, 1114, 1583
BstMBI	GATC	5 cut(s)	352, 529, 639, 1111, 1580
BstMCI	CGRYCG	2 cut(s)	29, 849
BstMWI	GCNNNNNNNGC	4 cut(s)	166, 263, 305, 311
BstNI	CCWGG	1 cut(s)	1390
BstNSI	RCATGY	1 cut(s)	173
BstSCI	CCNGG	3 cut(s)	198, 199, 1388
BstSFI	CTRYAG	2 cut(s)	525, 1192
BstSLI	GKGCMC	1 cut(s)	522
BstUI	CGCG	1 cut(s)	1599
BstV1I	GCAGC	7 cut(s)	241, 256, 292, 295, 298, 522, 1243
BstV2I	GAAGAC	1 cut(s)	543
BstZI	CGGCCG	2 cut(s)	26, 846
BsuRI	GGCC	7 cut(s)	28, 182, 848, 1097, 1226, 1388, 1393
BtrI	CACGTC	1 cut(s)	15
BtsCI	GGATG	2 cut(s)	48, 820
BtsIMutI	CAGTG	5 cut(s)	105, 295, 486, 702, 1129
BtuMI	TCGCGA	1 cut(s)	1599
Cac8I	GCNNGC	3 cut(s)	1058, 1192, 1317
CciI	TCATGA	1 cut(s)	532
CfoI	GCGC	3 cut(s)	214, 1056, 1321
Cfr10I	RCCGGY	2 cut(s)	165, 626
Cfr13I	GGNCC	3 cut(s)	1095, 1209, 1225
Cfr9I	CCCGGG	1 cut(s)	199
Csp6I	GTAC	3 cut(s)	190, 1164, 1363
CviAII	CATG	4 cut(s)	83, 170, 533, 1077
CviQI	GTAC	3 cut(s)	190, 1164, 1363
DdeI	CTNAG	6 cut(s)	564, 1019, 1032, 1041, 1117, 1461
DpnI	GATC	5 cut(s)	354, 531, 641, 1113, 1582
DpnII	GATC	5 cut(s)	352, 529, 639, 1111, 1580
EaeI	YGGCCR	2 cut(s)	26, 846
EagI	CGGCCG	2 cut(s)	26, 846
Eam1104I	CTCTTC	1 cut(s)	399
EarI	CTCTTC	1 cut(s)	399
Ecl136II	GAGCTC	2 cut(s)	622, 1491
EclXI	CGGCCG	2 cut(s)	26, 846
Eco130I	CCWWGG	1 cut(s)	1537
Eco24I	GRGCYC	2 cut(s)	624, 1493
Eco47I	GGWCC	1 cut(s)	1209
Eco52I	CGGCCG	2 cut(s)	26, 846
Eco53kI	GAGCTC	2 cut(s)	622, 1491
Eco57I	CTGAAG	2 cut(s)	399, 1266
Eco88I	CYCGRG	2 cut(s)	89, 199
EcoICRI	GAGCTC	2 cut(s)	622, 1491
EcoO109I	RGGNCCY	1 cut(s)	1209
EcoRI	GAATTC	1 cut(s)	556
EcoRII	CCWGG	1 cut(s)	1388
EcoT14I	CCWWGG	1 cut(s)	1537
EcoT38I	GRGCYC	2 cut(s)	624, 1493
ErhI	CCWWGG	1 cut(s)	1537
FaeI	CATG	4 cut(s)	86, 173, 536, 1080
FalI	AAGNNNNNCTT	2 cut(s)	212, 244
FaqI	GGGAC	2 cut(s)	752, 1195
FatI	CATG	4 cut(s)	82, 169, 532, 1076
FauI	CCCGC	1 cut(s)	203
FblI	GTMKAC	2 cut(s)	149, 426
FokI	GGATG	2 cut(s)	35, 827
FriOI	GRGCYC	2 cut(s)	624, 1493
FspBI	CTAG	4 cut(s)	798, 930, 981, 1509
FspI	TGCGCA	1 cut(s)	213
GlaI	GCGC	3 cut(s)	213, 1055, 1320
GsuI	CTGGAG	1 cut(s)	878
HaeIII	GGCC	7 cut(s)	28, 182, 848, 1097, 1226, 1388, 1393
HapII	CCGG	5 cut(s)	45, 166, 200, 627, 845
HhaI	GCGC	3 cut(s)	214, 1056, 1321
Hin1II	CATG	4 cut(s)	86, 173, 536, 1080
Hin6I	GCGC	3 cut(s)	212, 1054, 1319
HinP1I	GCGC	3 cut(s)	212, 1054, 1319
HincII	GTYRAC	1 cut(s)	150
HindII	GTYRAC	1 cut(s)	150
HindIII	AAGCTT	1 cut(s)	684
HinfI	GANTC	6 cut(s)	8, 79, 125, 676, 1024, 1471
HpaII	CCGG	5 cut(s)	45, 166, 200, 627, 845
HphI	GGTGA	3 cut(s)	616, 1225, 1416
Hpy166II	GTNNAC	6 cut(s)	150, 427, 520, 610, 909, 1157
Hpy188I	TCNGA	7 cut(s)	21, 124, 343, 379, 882, 1020, 1084
Hpy8I	GTNNAC	6 cut(s)	150, 427, 520, 610, 909, 1157
Hpy99I	CGWCG	2 cut(s)	16, 392
HpyAV	CCTTC	8 cut(s)	351, 410, 415, 1086, 1240, 1426, 1438, 1536
HpyCH4III	ACNGT	3 cut(s)	946, 1367, 1483
HpyCH4IV	ACGT	1 cut(s)	14
HpyCH4V	TGCA	7 cut(s)	314, 482, 520, 1194, 1259, 1295, 1315
HpyF10VI	GCNNNNNNNGC	4 cut(s)	166, 263, 305, 311
HpyF3I	CTNAG	6 cut(s)	564, 1019, 1032, 1041, 1117, 1461
HpySE526I	ACGT	1 cut(s)	14
Hsp92II	CATG	4 cut(s)	86, 173, 536, 1080
HspAI	GCGC	3 cut(s)	212, 1054, 1319
Kzo9I	GATC	5 cut(s)	352, 529, 639, 1111, 1580
LmnI	GCTCC	3 cut(s)	619, 799, 1514
Lsp1109I	GCAGC	7 cut(s)	241, 256, 292, 295, 298, 522, 1243
LweI	GCATC	2 cut(s)	12, 57
MaeI	CTAG	4 cut(s)	798, 930, 981, 1509
MaeII	ACGT	1 cut(s)	14
MaeIII	GTNAC	2 cut(s)	665, 1453
MalI	GATC	5 cut(s)	354, 531, 641, 1113, 1582
MboI	GATC	5 cut(s)	352, 529, 639, 1111, 1580
MhlI	GDGCHC	3 cut(s)	522, 624, 1493
MluCI	AATT	3 cut(s)	500, 556, 888
MlyI	GAGTC	2 cut(s)	134, 1018
MmeI	TCCRAC	3 cut(s)	102, 803, 1052
MseI	TTAA	4 cut(s)	743, 1238, 1290, 1587
MslI	CAYNNNNRTG	1 cut(s)	491
MspA1I	CMGCKG	2 cut(s)	269, 563
MspI	CCGG	5 cut(s)	45, 166, 200, 627, 845
MspR9I	CCNGG	3 cut(s)	200, 201, 1390
Mva1269I	GAATGC	2 cut(s)	455, 1060
MvaI	CCWGG	1 cut(s)	1390
MvnI	CGCG	1 cut(s)	1599
MwoI	GCNNNNNNNGC	4 cut(s)	166, 263, 305, 311
NciI	CCSGG	2 cut(s)	200, 201
NdeII	GATC	5 cut(s)	352, 529, 639, 1111, 1580
NlaIII	CATG	4 cut(s)	86, 173, 536, 1080
NlaIV	GGNNCC	5 cut(s)	74, 1129, 1211, 1227, 1449
NmeAIII	GCCGAG	1 cut(s)	208
NruI	TCGCGA	1 cut(s)	1599
NsbI	TGCGCA	1 cut(s)	213
NspI	RCATGY	1 cut(s)	173
PagI	TCATGA	1 cut(s)	532
PcsI	WCGNNNNNNNCGW	2 cut(s)	63, 114
PctI	GAATGC	2 cut(s)	455, 1060
PfeI	GAWTC	4 cut(s)	8, 79, 676, 1471
PflMI	CCANNNNNTGG	1 cut(s)	547
PleI	GAGTC	2 cut(s)	133, 1018
PpsI	GAGTC	2 cut(s)	133, 1018
PpuMI	RGGWCCY	1 cut(s)	1209
Psp124BI	GAGCTC	2 cut(s)	624, 1493
Psp5II	RGGWCCY	1 cut(s)	1209
Psp6I	CCWGG	1 cut(s)	1388
PspGI	CCWGG	1 cut(s)	1388
PspN4I	GGNNCC	5 cut(s)	74, 1129, 1211, 1227, 1449
PspPI	GGNCC	3 cut(s)	1095, 1209, 1225
PspPPI	RGGWCCY	1 cut(s)	1209
PsrI	GAACNNNNNNTAC	2 cut(s)	1156, 1188
PstI	CTGCAG	1 cut(s)	1196
PvuII	CAGCTG	1 cut(s)	563
RruI	TCGCGA	1 cut(s)	1599
RsaI	GTAC	3 cut(s)	191, 1165, 1364
RsaNI	GTAC	3 cut(s)	190, 1164, 1363
RseI	CAYNNNNRTG	1 cut(s)	491
SacI	GAGCTC	2 cut(s)	624, 1493
SalI	GTCGAC	1 cut(s)	148
SaqAI	TTAA	4 cut(s)	743, 1238, 1290, 1587
Sau3AI	GATC	5 cut(s)	352, 529, 639, 1111, 1580
Sau96I	GGNCC	3 cut(s)	1095, 1209, 1225
SchI	GAGTC	2 cut(s)	134, 1018
ScrFI	CCNGG	3 cut(s)	200, 201, 1390
SduI	GDGCHC	3 cut(s)	522, 624, 1493
SfaNI	GCATC	2 cut(s)	12, 57
SfcI	CTRYAG	2 cut(s)	525, 1192
SinI	GGWCC	1 cut(s)	1209
SmaI	CCCGGG	1 cut(s)	201
SmiMI	CAYNNNNRTG	1 cut(s)	491
SmlI	CTYRAG	2 cut(s)	967, 1331
SmoI	CTYRAG	2 cut(s)	967, 1331
Sse9I	AATT	3 cut(s)	500, 556, 888
SsiI	CCGC	6 cut(s)	25, 196, 267, 272, 327, 849
SspI	AATATT	1 cut(s)	1618
SspMI	CTAG	4 cut(s)	798, 930, 981, 1509
SstI	GAGCTC	2 cut(s)	624, 1493
StyD4I	CCNGG	3 cut(s)	198, 199, 1388
StyI	CCWWGG	1 cut(s)	1537
TaaI	ACNGT	3 cut(s)	946, 1367, 1483
TaiI	ACGT	1 cut(s)	17
TasI	AATT	3 cut(s)	500, 556, 888
TauI	GCSGC	4 cut(s)	28, 269, 329, 851
TfiI	GAWTC	4 cut(s)	8, 79, 676, 1471
Tru1I	TTAA	4 cut(s)	743, 1238, 1290, 1587
Tru9I	TTAA	4 cut(s)	743, 1238, 1290, 1587
TscAI	CASTG	5 cut(s)	105, 295, 493, 702, 1129
TseI	GCWGC	7 cut(s)	254, 269, 305, 308, 311, 510, 1256
TspDTI	ATGAA	3 cut(s)	71, 549, 1209
TspGWI	ACGGA	2 cut(s)	118, 1151
TspMI	CCCGGG	1 cut(s)	199
TspRI	CASTG	5 cut(s)	105, 295, 493, 702, 1129
Van91I	CCANNNNNTGG	1 cut(s)	547
VneI	GTGCAC	1 cut(s)	518
VpaK11BI	GGWCC	1 cut(s)	1209
XapI	RAATTY	2 cut(s)	500, 556
XceI	RCATGY	1 cut(s)	173
XmaI	CCCGGG	1 cut(s)	199
XmiI	GTMKAC	2 cut(s)	149, 426
XspI	CTAG	4 cut(s)	798, 930, 981, 1509

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.