Rh4DG312500

TPX2 (targeting protein for Xklp2) protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001269
Physical Location & Seq
Forward (+)
53528080 .. 53531339
3260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG312500.1

Sequence Viewer

Length: 1614 bp
ATGGGTGACTCTTCGTGTCTAATGAGGTCGTTTTCTGGGCCTTCTGAGCGTTCCGGTGATGTCAAAGAGGGGGGCCTCCTTCGGTGTCTAGGAGAATCAGTCTCATTCGGTAGGTATGCTTCAGAACCTCTTGATTGGGAAAAATGGTCGACATTTTCTCACAATCGGTATCTGGAAGAAGTGGAGAAGTTTTCCAAACCGGGCTCGGTTGCACAGAAGAAAGCCTACTTTGAAGCACACTACAAAAAGAAGGCTGCTGAGAAAGCAGCAGCTTTGGCTGAGGTCGCAAATACTTGTGATAGCAAAGAACCCGAGTCAGTTTCTATGCACAAGAGTTGTCAAAGCTCCTCTGTCAACATGGAGTTGGCAAATGGAGAGAGCCTGATGGTTGAGGAAAATATAGTTCCAAGTACTGAGGTGGTATACTCAGCTGTTGTGAATGACCACAATCCAGATGTTGAAGGGACTCAATTGGAAAATGCAAAGGTGGATGGAGCAGAGGCGGTCATTCAAGAAAGCGTTACCGTGGAGGATGCTATTCAGGTTGAGGTGGAGAATTCTATGCAGGATGAAATGGATACTTCGATTCAGGTTCAGATGGAGAGTTCTACTCGAGTTGAAATCTCAAACCAGTTTGAAAATGACCAAAAAGAGATTGTTAACGGTGCCACCCAAGAAGAGAAGATGCCTGATAAGGAAGCTGCGAGTGAGGAAAATCTAGCTTCAACAAGCAAGAAAAGATCGGTGAACTCTTCTCCCTCAAGGTTATCTACTCAATGTAGAGCATCCAAGATCCAATCTTTTCCTGCCAAACAACTTGCTGGTGTTCCAACTGGAAAGAAGAACAATGCCACCCCAAAGAGTAAGAATTCTGAGGCTGACTTAGTTGACAAGAGGAGATCAACAAAAAAATCACTCCACATGTCAATCAATTTCAGCTCTCATGCTGGTGAAACCAGTAAAAGAACTTCGCCGTTCCTGGAGAAGATAAAAAACTTAAGAGTTGATGCTACTGCACTTAATGTGCCTGTTAAAAACTCAGCTTCCCTACAAACTGCAACTAGGGCATCTGTTAATGGGGTATTGAAGCAATCTTCAGTAAACCCATGGTCCAAAGATAGAAGTCAATCTCTTGCTGCAAATGGAAGCAAACCACGAGGTCCTATCAAGTCCTGTCCTTTTAGCCTTAGGAGTGAAGAAAGAGCAGCAAAACGGAAAGAGTTCTTTCAGAGGCTGGAAGAGAAGAAAAATGGAGAGACAGAAAGTAAGCAGCTGCCAATGAGATCTAATAAGGAAAAAGCGGTGAATGATGTTAAAAAATTGCGTCAGAGTACAGGCTTAAAAGCCAAAGATCTGTCTTCTGGATCACAATTGCTAAATAATAAAGTGAAGAAGATGACACTTATACAGGTTCCACCGACCCGGAGTCAGTCACAGAAACTCGAGAGGCAGTCTACTACCAGCAAGGTCTTGGACCCAAGCACTCGGCCTTTGGTCAATTCTCACAGCTCTAAGCATGTCACACAGAAATATAACCCAAGGACCAGCCAGTCAGTCACTTCCCTTCCAAAGAAGAATGCTCATGAGAATTCTTCTCCCAATATTCAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

537

Amino Acids

59.08

Weight (kDa)

9.25

Isoelectric Point (pI)

58.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 394 - 453 1.5e-11 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1549
AccB1I GGYRCC 1 cut(s) 665
AccI GTMKAC 3 cut(s) 149, 423, 1454
AciI CCGC 2 cut(s) 503, 1301
AclWI GGATC 2 cut(s) 787, 1372
AcsI RAATTY 3 cut(s) 556, 868, 1588
AcuI CTGAAG 2 cut(s) 105, 1080
AfaI GTAC 2 cut(s) 412, 1333
AflII CTTAAG 1 cut(s) 997
AflIII ACRYGT 1 cut(s) 921
AgsI TTSAA 7 cut(s) 233, 461, 512, 620, 638, 726, 1087
AhdI GACNNNNNGTC 1 cut(s) 1425
AjnI CCWGG 1 cut(s) 978
AluBI AGCT 9 cut(s) 272, 345, 431, 701, 722, 939, 1043, 1273, 1509
AluI AGCT 9 cut(s) 272, 345, 431, 701, 722, 939, 1043, 1273, 1509
Alw26I GTCTC 2 cut(s) 106, 1250
AlwI GGATC 2 cut(s) 787, 1372
AlwNI CAGNNNCTG 1 cut(s) 1234
Ama87I CYCGRG 3 cut(s) 311, 612, 1442
AoxI GGCC 3 cut(s) 38, 73, 1487
ApeKI GCWGC 8 cut(s) 254, 266, 269, 701, 1136, 1205, 1270, 1273
ApoI RAATTY 3 cut(s) 556, 868, 1588
Asp700I GAANNNNTTC 1 cut(s) 1220
AspS9I GGNCC 6 cut(s) 38, 73, 1110, 1160, 1474, 1542
AsuC2I CCSGG 2 cut(s) 201, 1423
AsuHPI GGTGA 5 cut(s) 17, 68, 757, 962, 1315
AvaI CYCGRG 3 cut(s) 311, 612, 1442
AvaII GGWCC 4 cut(s) 1110, 1160, 1474, 1542
AxyI CCTNAGG 1 cut(s) 1187
BanI GGYRCC 1 cut(s) 665
BanII GRGCYC 1 cut(s) 206
BarI GAAGNNNNNNTAC 6 cut(s) 103, 135, 209, 241, 952, 984
BauI CACGAG 1 cut(s) 1155
BbsI GAAGAC 1 cut(s) 1350
BbvCI CCTCAGC 1 cut(s) 279
BbvI GCAGC 8 cut(s) 241, 278, 281, 688, 1123, 1217, 1260, 1282
BccI CCATC 3 cut(s) 379, 485, 592
BceAI ACGGC 1 cut(s) 958
BciT130I CCWGG 1 cut(s) 980
BciVI GTATCC 1 cut(s) 571
BcnI CCSGG 2 cut(s) 201, 1423
BcoDI GTCTC 2 cut(s) 106, 1250
BfaI CTAG 3 cut(s) 89, 719, 1062
BfrI CTTAAG 1 cut(s) 997
BfuI GTATCC 1 cut(s) 571
BglII AGATCT 2 cut(s) 1283, 1351
BisI GCNGC 8 cut(s) 255, 267, 270, 702, 1137, 1206, 1271, 1274
BlsI GCNGC 8 cut(s) 256, 268, 271, 703, 1138, 1207, 1272, 1275
BmcAI AGTACT 1 cut(s) 412
Bme1390I CCNGG 3 cut(s) 201, 980, 1423
Bme18I GGWCC 4 cut(s) 1110, 1160, 1474, 1542
BmeRI GACNNNNNGTC 1 cut(s) 1425
BmeT110I CYCGRG 3 cut(s) 311, 612, 1442
BmgT120I GGNCC 6 cut(s) 38, 73, 1110, 1160, 1474, 1542
BmiI GGNNCC 4 cut(s) 74, 667, 1413, 1476
BmrFI CCNGG 3 cut(s) 201, 980, 1423
BmsI GCATC 5 cut(s) 523, 675, 794, 997, 1076
BpiI GAAGAC 1 cut(s) 1350
BplI GAGNNNNNCTC 2 cut(s) 595, 627
BpmI CTGGAG 1 cut(s) 1001
Bpu10I CCTNAGC 1 cut(s) 279
BpuEI CTTGAG 1 cut(s) 745
BpuMI CCSGG 2 cut(s) 201, 1423
BsaJI CCNNGG 3 cut(s) 525, 1106, 1538
BsaWI WCCGGW 1 cut(s) 53
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bse1I ACTGG 4 cut(s) 631, 838, 957, 1549
Bse21I CCTNAGG 1 cut(s) 1187
BseBI CCWGG 1 cut(s) 980
BseDI CCNNGG 3 cut(s) 525, 1106, 1538
BseGI GGATG 4 cut(s) 496, 538, 574, 785
BseMII CTCAG 7 cut(s) 36, 249, 270, 405, 441, 864, 1053
BseNI ACTGG 4 cut(s) 631, 838, 957, 1549
BseRI GAGGAG 2 cut(s) 337, 910
BseXI GCAGC 8 cut(s) 241, 278, 281, 688, 1123, 1217, 1260, 1282
BsgI GTGCAG 1 cut(s) 999
BshFI GGCC 3 cut(s) 40, 75, 1489
BshNI GGYRCC 1 cut(s) 665
BsiHKCI CYCGRG 3 cut(s) 311, 612, 1442
BsiSI CCGG 3 cut(s) 54, 200, 1423
BslFI GGGAC 1 cut(s) 478
BsmAI GTCTC 2 cut(s) 106, 1250
BsmFI GGGAC 1 cut(s) 478
BsmI GAATGC 1 cut(s) 1582
BsnI GGCC 3 cut(s) 40, 75, 1489
BsoBI CYCGRG 3 cut(s) 311, 612, 1442
Bsp1286I GDGCHC 1 cut(s) 206
Bsp143I GATC 6 cut(s) 740, 792, 899, 1283, 1351, 1364
Bsp19I CCATGG 1 cut(s) 1106
BspACI CCGC 2 cut(s) 503, 1301
BspANI GGCC 3 cut(s) 40, 75, 1489
BspCNI CTCAG 7 cut(s) 37, 250, 271, 406, 440, 865, 1052
BspHI TCATGA 1 cut(s) 1582
BspLI GGNNCC 4 cut(s) 74, 667, 1413, 1476
BspPI GGATC 2 cut(s) 787, 1372
BspT107I GGYRCC 1 cut(s) 665
BspTI CTTAAG 1 cut(s) 997
BsrI ACTGG 4 cut(s) 631, 838, 957, 1549
BssECI CCNNGG 3 cut(s) 525, 1106, 1538
BssMI GATC 6 cut(s) 740, 792, 899, 1283, 1351, 1364
BssNAI GTATAC 1 cut(s) 424
BssSI CACGAG 1 cut(s) 1155
BssT1I CCWWGG 2 cut(s) 1106, 1538
Bst1107I GTATAC 1 cut(s) 424
Bst2BI CACGAG 1 cut(s) 1155
Bst2UI CCWGG 1 cut(s) 980
Bst4CI ACNGT 2 cut(s) 526, 665
Bst6I CTCTTC 4 cut(s) 16, 672, 757, 1233
BstAFI CTTAAG 1 cut(s) 997
BstDSI CCRYGG 2 cut(s) 525, 1106
BstF5I GGATG 4 cut(s) 496, 538, 574, 785
BstKTI GATC 6 cut(s) 743, 795, 902, 1286, 1354, 1367
BstMAI GTCTC 2 cut(s) 106, 1250
BstMBI GATC 6 cut(s) 740, 792, 899, 1283, 1351, 1364
BstMWI GCNNNNNNNGC 5 cut(s) 46, 263, 275, 284, 1064
BstNI CCWGG 1 cut(s) 980
BstNSI RCATGY 2 cut(s) 925, 1520
BstSCI CCNGG 3 cut(s) 199, 978, 1421
BstV1I GCAGC 8 cut(s) 241, 278, 281, 688, 1123, 1217, 1260, 1282
BstV2I GAAGAC 1 cut(s) 1350
BstX2I RGATCY 3 cut(s) 792, 1283, 1351
BstYI RGATCY 3 cut(s) 792, 1283, 1351
BstZ17I GTATAC 1 cut(s) 424
Bsu36I CCTNAGG 1 cut(s) 1187
BsuI GTATCC 1 cut(s) 571
BsuRI GGCC 3 cut(s) 40, 75, 1489
BtgI CCRYGG 2 cut(s) 525, 1106
BtsCI GGATG 4 cut(s) 496, 538, 574, 785
CaiI CAGNNNCTG 1 cut(s) 1234
CciI TCATGA 1 cut(s) 1582
Cfr13I GGNCC 6 cut(s) 38, 73, 1110, 1160, 1474, 1542
CseI GACGC 1 cut(s) 1313
Csp6I GTAC 2 cut(s) 411, 1332
CviAII CATG 6 cut(s) 358, 922, 944, 1107, 1517, 1583
CviQI GTAC 2 cut(s) 411, 1332
DpnI GATC 6 cut(s) 742, 794, 901, 1285, 1353, 1366
DpnII GATC 6 cut(s) 740, 792, 899, 1283, 1351, 1364
DrdI GACNNNNNNGTC 1 cut(s) 1549
DriI GACNNNNNGTC 1 cut(s) 1425
DseDI GACNNNNNNGTC 1 cut(s) 1549
Eam1104I CTCTTC 4 cut(s) 16, 672, 757, 1233
Eam1105I GACNNNNNGTC 1 cut(s) 1425
EarI CTCTTC 4 cut(s) 16, 672, 757, 1233
Eco130I CCWWGG 2 cut(s) 1106, 1538
Eco24I GRGCYC 1 cut(s) 206
Eco47I GGWCC 4 cut(s) 1110, 1160, 1474, 1542
Eco57I CTGAAG 2 cut(s) 105, 1080
Eco81I CCTNAGG 1 cut(s) 1187
Eco88I CYCGRG 3 cut(s) 311, 612, 1442
EcoO109I RGGNCCY 2 cut(s) 73, 1160
EcoRI GAATTC 3 cut(s) 556, 868, 1588
EcoRII CCWGG 1 cut(s) 978
EcoT14I CCWWGG 2 cut(s) 1106, 1538
EcoT38I GRGCYC 1 cut(s) 206
ErhI CCWWGG 2 cut(s) 1106, 1538
FaeI CATG 6 cut(s) 361, 925, 947, 1110, 1520, 1586
FaqI GGGAC 1 cut(s) 478
FatI CATG 6 cut(s) 357, 921, 943, 1106, 1516, 1582
FblI GTMKAC 3 cut(s) 149, 423, 1454
Fnu4HI GCNGC 8 cut(s) 255, 267, 270, 702, 1137, 1206, 1271, 1274
FokI GGATG 4 cut(s) 503, 545, 581, 772
FriOI GRGCYC 1 cut(s) 206
Fsp4HI GCNGC 8 cut(s) 255, 267, 270, 702, 1137, 1206, 1271, 1274
FspBI CTAG 3 cut(s) 89, 719, 1062
GluI GCNGC 8 cut(s) 255, 267, 270, 702, 1137, 1206, 1271, 1274
GsuI CTGGAG 1 cut(s) 1001
HaeIII GGCC 3 cut(s) 40, 75, 1489
HapII CCGG 3 cut(s) 54, 200, 1423
HgaI GACGC 1 cut(s) 1313
Hin1II CATG 6 cut(s) 361, 925, 947, 1110, 1520, 1586
HincII GTYRAC 4 cut(s) 150, 355, 661, 889
HindII GTYRAC 4 cut(s) 150, 355, 661, 889
HinfI GANTC 6 cut(s) 8, 95, 314, 466, 586, 1426
HpaI GTTAAC 1 cut(s) 661
HpaII CCGG 3 cut(s) 54, 200, 1423
HphI GGTGA 5 cut(s) 17, 68, 757, 962, 1315
Hpy166II GTNNAC 8 cut(s) 150, 355, 424, 661, 748, 889, 1102, 1455
Hpy188I TCNGA 7 cut(s) 46, 124, 597, 874, 1230, 1329, 1608
Hpy188III TCNNGA 7 cut(s) 131, 173, 452, 512, 1362, 1444, 1583
Hpy8I GTNNAC 8 cut(s) 150, 355, 424, 661, 748, 889, 1102, 1455
HpyAV CCTTC 5 cut(s) 51, 89, 244, 455, 1574
HpyCH4III ACNGT 2 cut(s) 526, 665
HpyCH4V TGCA 7 cut(s) 212, 328, 482, 565, 1016, 1058, 1139
HpyF10VI GCNNNNNNNGC 5 cut(s) 46, 263, 275, 284, 1064
Hsp92II CATG 6 cut(s) 361, 925, 947, 1110, 1520, 1586
KspAI GTTAAC 1 cut(s) 661
Kzo9I GATC 6 cut(s) 740, 792, 899, 1283, 1351, 1364
LmnI GCTCC 2 cut(s) 350, 494
Lsp1109I GCAGC 8 cut(s) 241, 278, 281, 688, 1123, 1217, 1260, 1282
LweI GCATC 5 cut(s) 523, 675, 794, 997, 1076
MaeI CTAG 3 cut(s) 89, 719, 1062
MaeIII GTNAC 5 cut(s) 5, 520, 1431, 1519, 1555
MalI GATC 6 cut(s) 742, 794, 901, 1285, 1353, 1366
MboI GATC 6 cut(s) 740, 792, 899, 1283, 1351, 1364
MfeI CAATTG 2 cut(s) 470, 1370
MflI RGATCY 3 cut(s) 792, 1283, 1351
MhlI GDGCHC 1 cut(s) 206
MluCI AATT 8 cut(s) 470, 556, 868, 931, 1319, 1370, 1498, 1588
MlyI GAGTC 4 cut(s) 2, 323, 460, 1435
MmeI TCCRAC 1 cut(s) 854
MroXI GAANNNNTTC 1 cut(s) 1220
MseI TTAA 7 cut(s) 660, 998, 1020, 1032, 1074, 1314, 1340
MslI CAYNNNNRTG 1 cut(s) 948
MspA1I CMGCKG 2 cut(s) 431, 1273
MspCI CTTAAG 1 cut(s) 997
MspI CCGG 3 cut(s) 54, 200, 1423
MspR9I CCNGG 3 cut(s) 201, 980, 1423
MunI CAATTG 2 cut(s) 470, 1370
Mva1269I GAATGC 1 cut(s) 1582
MvaI CCWGG 1 cut(s) 980
MwoI GCNNNNNNNGC 5 cut(s) 46, 263, 275, 284, 1064
NciI CCSGG 2 cut(s) 201, 1423
NcoI CCATGG 1 cut(s) 1106
NdeII GATC 6 cut(s) 740, 792, 899, 1283, 1351, 1364
NlaIII CATG 6 cut(s) 361, 925, 947, 1110, 1520, 1586
NlaIV GGNNCC 4 cut(s) 74, 667, 1413, 1476
NmeAIII GCCGAG 1 cut(s) 1465
NmuCI GTSAC 4 cut(s) 5, 1431, 1519, 1555
NspI RCATGY 2 cut(s) 925, 1520
PaeR7I CTCGAG 2 cut(s) 612, 1442
PagI TCATGA 1 cut(s) 1582
PciI ACATGT 1 cut(s) 921
PctI GAATGC 1 cut(s) 1582
PdmI GAANNNNTTC 1 cut(s) 1220
PfeI GAWTC 2 cut(s) 95, 586
PfoI TCCNGGA 1 cut(s) 978
PkrI GCNGC 8 cut(s) 256, 268, 271, 703, 1138, 1207, 1272, 1275
PleI GAGTC 4 cut(s) 2, 322, 460, 1434
PpsI GAGTC 4 cut(s) 2, 322, 460, 1434
PpuMI RGGWCCY 1 cut(s) 1160
PscI ACATGT 1 cut(s) 921
Psp5II RGGWCCY 1 cut(s) 1160
Psp6I CCWGG 1 cut(s) 978
PspGI CCWGG 1 cut(s) 978
PspN4I GGNNCC 4 cut(s) 74, 667, 1413, 1476
PspPI GGNCC 6 cut(s) 38, 73, 1110, 1160, 1474, 1542
PspPPI RGGWCCY 1 cut(s) 1160
PspXI VCTCGAGB 1 cut(s) 612
PstNI CAGNNNCTG 1 cut(s) 1234
PsuI RGATCY 3 cut(s) 792, 1283, 1351
PvuII CAGCTG 2 cut(s) 431, 1273
RsaI GTAC 2 cut(s) 412, 1333
RsaNI GTAC 2 cut(s) 411, 1332
RseI CAYNNNNRTG 1 cut(s) 948
SalI GTCGAC 1 cut(s) 148
SaqAI TTAA 7 cut(s) 660, 998, 1020, 1032, 1074, 1314, 1340
SatI GCNGC 8 cut(s) 255, 267, 270, 702, 1137, 1206, 1271, 1274
Sau3AI GATC 6 cut(s) 740, 792, 899, 1283, 1351, 1364
Sau96I GGNCC 6 cut(s) 38, 73, 1110, 1160, 1474, 1542
ScaI AGTACT 1 cut(s) 412
SchI GAGTC 4 cut(s) 2, 323, 460, 1435
ScrFI CCNGG 3 cut(s) 201, 980, 1423
SduI GDGCHC 1 cut(s) 206
SfaNI GCATC 5 cut(s) 523, 675, 794, 997, 1076
Sfr274I CTCGAG 2 cut(s) 612, 1442
SinI GGWCC 4 cut(s) 1110, 1160, 1474, 1542
SlaI CTCGAG 2 cut(s) 612, 1442
SmiMI CAYNNNNRTG 1 cut(s) 948
SmlI CTYRAG 4 cut(s) 612, 760, 997, 1442
SmoI CTYRAG 4 cut(s) 612, 760, 997, 1442
Sse9I AATT 8 cut(s) 470, 556, 868, 931, 1319, 1370, 1498, 1588
SsiI CCGC 2 cut(s) 503, 1301
SspI AATATT 1 cut(s) 1603
SspMI CTAG 3 cut(s) 89, 719, 1062
StyD4I CCNGG 3 cut(s) 199, 978, 1421
StyI CCWWGG 2 cut(s) 1106, 1538
TaaI ACNGT 2 cut(s) 526, 665
TaqI TCGA 4 cut(s) 149, 584, 613, 1443
TasI AATT 8 cut(s) 470, 556, 868, 931, 1319, 1370, 1498, 1588
TatI WGTACW 2 cut(s) 410, 1331
TfiI GAWTC 2 cut(s) 95, 586
Tru1I TTAA 7 cut(s) 660, 998, 1020, 1032, 1074, 1314, 1340
Tru9I TTAA 7 cut(s) 660, 998, 1020, 1032, 1074, 1314, 1340
TseFI GTSAC 4 cut(s) 5, 1431, 1519, 1555
TseI GCWGC 8 cut(s) 254, 266, 269, 701, 1136, 1205, 1270, 1273
Tsp45I GTSAC 4 cut(s) 5, 1431, 1519, 1555
TspDTI ATGAA 1 cut(s) 585
TspGWI ACGGA 1 cut(s) 1228
Vha464I CTTAAG 1 cut(s) 997
VpaK11BI GGWCC 4 cut(s) 1110, 1160, 1474, 1542
XapI RAATTY 3 cut(s) 556, 868, 1588
XceI RCATGY 2 cut(s) 925, 1520
XcmI CCANNNNNNNNNTGG 1 cut(s) 1468
XhoI CTCGAG 2 cut(s) 612, 1442
XmiI GTMKAC 3 cut(s) 149, 423, 1454
XmnI GAANNNNTTC 1 cut(s) 1220
XspI CTAG 3 cut(s) 89, 719, 1062
ZrmI AGTACT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.