MD13G1108400.v1.1

TPX2 (targeting protein for Xklp2) protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001269
Physical Location & Seq
Reverse (-)
7787012 .. 7790968
3957 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1108400.v1.1.491

Sequence Viewer

Length: 1668 bp
ATGGGTGAATCGGCCTGCCTGATGAGCTCATCATCCGACCCTTCTGAGTGTTCCCCTGAAGTCAAAGAGGGCGACCCCCTTCACTGCCTAGGAGAATCGATCTCCTTCGGTAGGTATATGTCAGAACCTCTTGCTTGGGAGAAATGGTCAGCATTTTCCCACAATCGATACTTAGAAGAAGTCGAGAAGTTCTCCAAGCCCGGCTCTGTTGCTCTGAAGAAGGCTTATTTTGAAGCTCATTACAAGAAAAAAGCTGCCGAGAAAGCAGCAGCCTTGCTTGAGGTGACAAATGCTGATGCCTGTAATGTTACAGCATCATCGGTAACTGTGTACGGAAATTGTGAGAGCTCCTCCGTGGACATAGAACCGGCAAATGGAGGGAGCCAGATGGTTGTTGATAAAGAACAGGAAAATGTTGCTCCATATTCTGAGGTAGAAGTATACTCAGCTGATGTGAATAGCCATAATCAAAATGTTGAAGGGAATCAATTGGATATCACCAAGGTGGACGGATCAGAAGCGATGATTGAAGAAAGTGTTAATTTGGAAAATCCTATTCACGTTGAAATATCAAACCAGTTTAAAAATGGCAAAGACCAAAATGAGATTGTTGCCACCCTAGAAGAGAAGATGCCAGAGACGATCCTCCAGGAAGCTTCCGGTGAGGAGAATCTTGCTTCAATGAGCAAGAAAAGATTGATGAACTCTTCCCCAAGGTTATCCACTAAAGGTAGAGCACCCAAGGTGCCACCGTCTCCTGCCAAACAAGCAATTCGTGTGCAAACCATAAACGGGAAAAATGCCACTCAAATGGGTAAGAAGGTTTCATCAGACTTAGTTGACAAGAGGAGTTTGACTGCAAAATCTCTCCACATGTCAATGCACTTCACTTCTCATGTGGGCGAAAATGAAACCAGTAAAAGAACTTCACCAGTTGTTGAGAAGACCAGAAATTCGAGAAGTAAAATGACTAAACTTAATATCTCCATAAATGATCCGGCTTCTCATCGACGAACTGCTATTAGGGCATCTGTTGGCGGGGTATTAATGCAGTCTTCAGTAGAACCATGGTCCAAAGATAGAAGGGCAAAAACATTACTGAATAAGTCAGTAGCTGGACCAATTGGAGATGGGAAATGGATATCCTCCTCAAAAGACCAATCTTCTACTGCAAACGGAAGTAAACCACGACCCCCTATTGTATCATGTCCTTTTAGATTGAAGAGTGAAGAAAGAGCAGAAAAGCAGAAATTTCTCTTTCTTTTCCTGCACCAGTTCTATCAGAGGCTGGAAGAGAAGAAAATTGCCAAGGAGGCTGAACATAAGCTGTTGCAACTGAGATCTAATAAAAGGGAAAAAGCAGTGGGTGATGTTAATAAACTGCACCAGAGCACTGCCTTAAAAGCAAAACGAAACCAAGTTCTCTCCTCGGGACCACAATTGCCAAGTAATCACTTGAACAAGATTCCTCCGTCACAGTCTCGGTCACCCAAACTTGGGAGGCACTCTACCGTCAGCAAAATTCTGGACTCAAGCTCTCGACCTTTTGTCAATTCTCACAGCTCCAAGTGTGTCACACAAAAATACAATTTGAGCACAAATCGAATGGTGCCTTCTCTTTCAAAAAAGAATGCTCACGAGAATTCTCCTCCCAATATCCAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

556

Amino Acids

61.18

Weight (kDa)

9.37

Isoelectric Point (pI)

63.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 745, 1609
AccI GTMKAC 1 cut(s) 441
AciI CCGC 1 cut(s) 1038
AclWI GGATC 3 cut(s) 520, 637, 989
AcsI RAATTY 4 cut(s) 952, 1250, 1521, 1642
AcuI CTGAAG 3 cut(s) 78, 236, 1041
AfaI GTAC 1 cut(s) 332
AfiI CCNNNNNNNGG 5 cut(s) 111, 374, 792, 1496, 1497
AflIII ACRYGT 1 cut(s) 873
AgsI TTSAA 8 cut(s) 233, 479, 530, 566, 681, 1222, 1459, 1623
AhdI GACNNNNNGTC 1 cut(s) 1547
AjnI CCWGG 1 cut(s) 648
AleI CACNNNNGTG 1 cut(s) 503
Alw21I GWGCWC 5 cut(s) 29, 350, 739, 1394, 1598
Alw26I GTCTC 3 cut(s) 632, 759, 1485
AlwI GGATC 3 cut(s) 520, 637, 989
AlwNI CAGNNNCTG 2 cut(s) 1115, 1288
Ama87I CYCGRG 1 cut(s) 1429
AoxI GGCC 1 cut(s) 12
ApeKI GCWGC 3 cut(s) 254, 266, 269
ApoI RAATTY 4 cut(s) 952, 1250, 1521, 1642
ArsI GACNNNNNNTTYG 2 cut(s) 937, 969
AseI ATTAAT 1 cut(s) 1046
AspA2I CCTAGG 1 cut(s) 88
AspS9I GGNCC 3 cut(s) 1071, 1118, 1433
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 7 cut(s) 17, 295, 490, 674, 921, 1379, 1479
AvaI CYCGRG 1 cut(s) 1429
AvaII GGWCC 3 cut(s) 1071, 1118, 1433
AvrII CCTAGG 1 cut(s) 88
BanI GGYRCC 2 cut(s) 745, 1609
BanII GRGCYC 2 cut(s) 29, 350
BarI GAAGNNNNNNTAC 2 cut(s) 910, 942
BauI CACGAG 1 cut(s) 1637
BbsI GAAGAC 2 cut(s) 950, 1047
Bbv12I GWGCWC 5 cut(s) 29, 350, 739, 1394, 1598
BbvI GCAGC 3 cut(s) 241, 278, 281
BccI CCATC 2 cut(s) 382, 1124
BciT130I CCWGG 1 cut(s) 650
BcnI CCSGG 1 cut(s) 201
BcoDI GTCTC 3 cut(s) 632, 759, 1485
BfaI CTAG 2 cut(s) 89, 620
BglI GCCNNNNNGGC 1 cut(s) 1313
BglII AGATCT 1 cut(s) 1340
BisI GCNGC 3 cut(s) 255, 267, 270
BlnI CCTAGG 1 cut(s) 88
BlsI GCNGC 3 cut(s) 256, 268, 271
Bme1390I CCNGG 2 cut(s) 201, 650
Bme18I GGWCC 3 cut(s) 1071, 1118, 1433
BmeRI GACNNNNNGTC 1 cut(s) 1547
BmeT110I CYCGRG 1 cut(s) 1429
BmgT120I GGNCC 3 cut(s) 1071, 1118, 1433
BmiI GGNNCC 4 cut(s) 383, 747, 1434, 1611
BmrFI CCNGG 2 cut(s) 201, 650
BmsI GCATC 4 cut(s) 286, 323, 621, 1037
BpiI GAAGAC 2 cut(s) 950, 1047
BplI GAGNNNNNCTC 4 cut(s) 176, 208, 335, 367
BpmI CTGGAG 1 cut(s) 632
BpuEI CTTGAG 2 cut(s) 299, 1516
BpuMI CCSGG 1 cut(s) 201
Bsa29I ATCGAT 2 cut(s) 98, 166
BsaJI CCNNGG 8 cut(s) 88, 354, 501, 713, 741, 1067, 1308, 1428
BsaWI WCCGGW 1 cut(s) 659
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 5 cut(s) 111, 374, 792, 1496, 1497
Bse118I RCCGGY 1 cut(s) 367
Bse1I ACTGG 4 cut(s) 577, 915, 932, 1273
BseBI CCWGG 1 cut(s) 650
BseCI ATCGAT 2 cut(s) 98, 166
BseDI CCNNGG 8 cut(s) 88, 354, 501, 713, 741, 1067, 1308, 1428
BseGI GGATG 1 cut(s) 32
BseLI CCNNNNNNNGG 5 cut(s) 111, 374, 792, 1496, 1497
BseMII CTCAG 4 cut(s) 36, 420, 459, 1328
BseNI ACTGG 4 cut(s) 577, 915, 932, 1273
BseRI GAGGAG 6 cut(s) 340, 680, 862, 1138, 1417, 1638
BseXI GCAGC 3 cut(s) 241, 278, 281
BsgI GTGCAG 2 cut(s) 1253, 1367
BshFI GGCC 1 cut(s) 14
BshNI GGYRCC 2 cut(s) 745, 1609
BshVI ATCGAT 2 cut(s) 98, 166
BsiHKAI GWGCWC 5 cut(s) 29, 350, 739, 1394, 1598
BsiHKCI CYCGRG 1 cut(s) 1429
BsiSI CCGG 4 cut(s) 201, 368, 660, 998
BslFI GGGAC 1 cut(s) 1446
BslI CCNNNNNNNGG 5 cut(s) 111, 374, 792, 1496, 1497
BsmAI GTCTC 3 cut(s) 632, 759, 1485
BsmBI CGTCTC 2 cut(s) 632, 759
BsmFI GGGAC 1 cut(s) 1446
BsmI GAATGC 1 cut(s) 1636
BsnI GGCC 1 cut(s) 14
BsoBI CYCGRG 1 cut(s) 1429
Bsp1286I GDGCHC 5 cut(s) 29, 350, 739, 1394, 1598
Bsp143I GATC 5 cut(s) 99, 512, 642, 994, 1340
Bsp19I CCATGG 1 cut(s) 1067
BspACI CCGC 1 cut(s) 1038
BspANI GGCC 1 cut(s) 14
BspCNI CTCAG 4 cut(s) 37, 421, 458, 1329
BspDI ATCGAT 2 cut(s) 98, 166
BspLI GGNNCC 4 cut(s) 383, 747, 1434, 1611
BspPI GGATC 3 cut(s) 520, 637, 989
BspT107I GGYRCC 2 cut(s) 745, 1609
BsrFI RCCGGY 1 cut(s) 367
BsrI ACTGG 4 cut(s) 577, 915, 932, 1273
BssAI RCCGGY 1 cut(s) 367
BssECI CCNNGG 8 cut(s) 88, 354, 501, 713, 741, 1067, 1308, 1428
BssMI GATC 5 cut(s) 99, 512, 642, 994, 1340
BssNAI GTATAC 1 cut(s) 442
BssSI CACGAG 1 cut(s) 1637
BssT1I CCWWGG 6 cut(s) 88, 501, 713, 741, 1067, 1308
Bst1107I GTATAC 1 cut(s) 442
Bst2BI CACGAG 1 cut(s) 1637
Bst2UI CCWGG 1 cut(s) 650
Bst4CI ACNGT 4 cut(s) 328, 753, 1479, 1513
Bst6I CTCTTC 4 cut(s) 618, 712, 1217, 1287
BstC8I GCNNGC 1 cut(s) 16
BstDEI CTNAG 6 cut(s) 45, 172, 429, 445, 835, 1337
BstDSI CCRYGG 2 cut(s) 354, 1067
BstEII GGTNACC 1 cut(s) 1485
BstENI CCTNNNNNAGG 1 cut(s) 109
BstF5I GGATG 1 cut(s) 32
BstKTI GATC 5 cut(s) 102, 515, 645, 997, 1343
BstMAI GTCTC 3 cut(s) 632, 759, 1485
BstMBI GATC 5 cut(s) 99, 512, 642, 994, 1340
BstMWI GCNNNNNNNGC 6 cut(s) 24, 263, 767, 1025, 1313, 1403
BstNI CCWGG 1 cut(s) 650
BstNSI RCATGY 1 cut(s) 877
BstPI GGTNACC 1 cut(s) 1485
BstSCI CCNGG 2 cut(s) 199, 648
BstV1I GCAGC 3 cut(s) 241, 278, 281
BstV2I GAAGAC 2 cut(s) 950, 1047
BstX2I RGATCY 1 cut(s) 1340
BstXI CCANNNNNNTGG 1 cut(s) 811
BstYI RGATCY 1 cut(s) 1340
BstZ17I GTATAC 1 cut(s) 442
Bsu15I ATCGAT 2 cut(s) 98, 166
BsuRI GGCC 1 cut(s) 14
BsuTUI ATCGAT 2 cut(s) 98, 166
BtgI CCRYGG 2 cut(s) 354, 1067
BtgZI GCGATG 1 cut(s) 536
BtsCI GGATG 1 cut(s) 32
BtsI GCAGTG 3 cut(s) 82, 1368, 1392
BtsIMutI CAGTG 3 cut(s) 82, 1368, 1392
Cac8I GCNNGC 1 cut(s) 16
CaiI CAGNNNCTG 2 cut(s) 1115, 1288
Cfr10I RCCGGY 1 cut(s) 367
Cfr13I GGNCC 3 cut(s) 1071, 1118, 1433
ClaI ATCGAT 2 cut(s) 98, 166
Csp6I GTAC 1 cut(s) 331
CviAII CATG 4 cut(s) 874, 896, 1068, 1206
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 6 cut(s) 45, 172, 429, 445, 835, 1337
DpnI GATC 5 cut(s) 101, 514, 644, 996, 1342
DpnII GATC 5 cut(s) 99, 512, 642, 994, 1340
DraI TTTAAA 1 cut(s) 583
DriI GACNNNNNGTC 1 cut(s) 1547
Eam1104I CTCTTC 4 cut(s) 618, 712, 1217, 1287
Eam1105I GACNNNNNGTC 1 cut(s) 1547
EarI CTCTTC 4 cut(s) 618, 712, 1217, 1287
Ecl136II GAGCTC 2 cut(s) 27, 348
Eco130I CCWWGG 6 cut(s) 88, 501, 713, 741, 1067, 1308
Eco24I GRGCYC 2 cut(s) 29, 350
Eco32I GATATC 2 cut(s) 496, 1143
Eco47I GGWCC 3 cut(s) 1071, 1118, 1433
Eco53kI GAGCTC 2 cut(s) 27, 348
Eco57I CTGAAG 3 cut(s) 78, 236, 1041
Eco88I CYCGRG 1 cut(s) 1429
Eco91I GGTNACC 1 cut(s) 1485
EcoICRI GAGCTC 2 cut(s) 27, 348
EcoNI CCTNNNNNAGG 1 cut(s) 109
EcoO65I GGTNACC 1 cut(s) 1485
EcoRI GAATTC 1 cut(s) 1642
EcoRII CCWGG 1 cut(s) 648
EcoRV GATATC 2 cut(s) 496, 1143
EcoT14I CCWWGG 6 cut(s) 88, 501, 713, 741, 1067, 1308
EcoT38I GRGCYC 2 cut(s) 29, 350
ErhI CCWWGG 6 cut(s) 88, 501, 713, 741, 1067, 1308
Esp3I CGTCTC 2 cut(s) 632, 759
FaeI CATG 4 cut(s) 877, 899, 1071, 1209
FaqI GGGAC 1 cut(s) 1446
FatI CATG 4 cut(s) 873, 895, 1067, 1205
FauI CCCGC 1 cut(s) 1031
FblI GTMKAC 1 cut(s) 441
Fnu4HI GCNGC 3 cut(s) 255, 267, 270
FokI GGATG 1 cut(s) 19
FriOI GRGCYC 2 cut(s) 29, 350
Fsp4HI GCNGC 3 cut(s) 255, 267, 270
FspBI CTAG 2 cut(s) 89, 620
GluI GCNGC 3 cut(s) 255, 267, 270
GsuI CTGGAG 1 cut(s) 632
HaeIII GGCC 1 cut(s) 14
HapII CCGG 4 cut(s) 201, 368, 660, 998
Hin1II CATG 4 cut(s) 877, 899, 1071, 1209
HincII GTYRAC 1 cut(s) 841
HindII GTYRAC 1 cut(s) 841
HindIII AAGCTT 1 cut(s) 654
HinfI GANTC 6 cut(s) 8, 95, 484, 670, 1465, 1529
HpaII CCGG 4 cut(s) 201, 368, 660, 998
HphI GGTGA 7 cut(s) 17, 295, 490, 674, 921, 1379, 1479
Hpy166II GTNNAC 6 cut(s) 331, 358, 442, 508, 841, 1184
Hpy188I TCNGA 8 cut(s) 37, 46, 124, 216, 430, 517, 832, 1284
Hpy188III TCNNGA 7 cut(s) 184, 957, 1431, 1526, 1539, 1637, 1660
Hpy8I GTNNAC 6 cut(s) 331, 358, 442, 508, 841, 1184
Hpy99I CGWCG 1 cut(s) 1014
HpyAV CCTTC 8 cut(s) 51, 89, 115, 214, 473, 814, 1077, 1623
HpyCH4III ACNGT 4 cut(s) 328, 753, 1479, 1513
HpyCH4IV ACGT 1 cut(s) 561
HpyCH4V TGCA 8 cut(s) 781, 860, 883, 1051, 1172, 1270, 1333, 1384
HpyF10VI GCNNNNNNNGC 6 cut(s) 24, 263, 767, 1025, 1313, 1403
HpyF3I CTNAG 6 cut(s) 45, 172, 429, 445, 835, 1337
HpySE526I ACGT 1 cut(s) 561
Hsp92II CATG 4 cut(s) 877, 899, 1071, 1209
Kzo9I GATC 5 cut(s) 99, 512, 642, 994, 1340
LmnI GCTCC 4 cut(s) 353, 381, 424, 1568
Lsp1109I GCAGC 3 cut(s) 241, 278, 281
LweI GCATC 4 cut(s) 286, 323, 621, 1037
MaeI CTAG 2 cut(s) 89, 620
MaeII ACGT 1 cut(s) 561
MaeIII GTNAC 6 cut(s) 283, 307, 322, 1473, 1485, 1573
MalI GATC 5 cut(s) 101, 514, 644, 996, 1342
MboI GATC 5 cut(s) 99, 512, 642, 994, 1340
MfeI CAATTG 3 cut(s) 488, 1122, 1439
MflI RGATCY 1 cut(s) 1340
MhlI GDGCHC 5 cut(s) 29, 350, 739, 1394, 1598
MlyI GAGTC 1 cut(s) 1523
MmeI TCCRAC 1 cut(s) 60
MseI TTAA 6 cut(s) 540, 582, 978, 1046, 1374, 1400
MslI CAYNNNNRTG 3 cut(s) 503, 809, 878
MspA1I CMGCKG 1 cut(s) 449
MspI CCGG 4 cut(s) 201, 368, 660, 998
MspR9I CCNGG 2 cut(s) 201, 650
MunI CAATTG 3 cut(s) 488, 1122, 1439
Mva1269I GAATGC 1 cut(s) 1636
MvaI CCWGG 1 cut(s) 650
MwoI GCNNNNNNNGC 6 cut(s) 24, 263, 767, 1025, 1313, 1403
NciI CCSGG 1 cut(s) 201
NcoI CCATGG 1 cut(s) 1067
NdeII GATC 5 cut(s) 99, 512, 642, 994, 1340
NlaIII CATG 4 cut(s) 877, 899, 1071, 1209
NlaIV GGNNCC 4 cut(s) 383, 747, 1434, 1611
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 4 cut(s) 283, 1473, 1485, 1573
NspI RCATGY 1 cut(s) 877
OliI CACNNNNGTG 1 cut(s) 503
PciI ACATGT 1 cut(s) 873
PctI GAATGC 1 cut(s) 1636
PfeI GAWTC 5 cut(s) 8, 95, 484, 670, 1465
PfoI TCCNGGA 1 cut(s) 648
PkrI GCNGC 3 cut(s) 256, 268, 271
PleI GAGTC 1 cut(s) 1523
PpsI GAGTC 1 cut(s) 1523
PscI ACATGT 1 cut(s) 873
PshBI ATTAAT 1 cut(s) 1046
Psp124BI GAGCTC 2 cut(s) 29, 350
Psp6I CCWGG 1 cut(s) 648
PspEI GGTNACC 1 cut(s) 1485
PspGI CCWGG 1 cut(s) 648
PspN4I GGNNCC 4 cut(s) 383, 747, 1434, 1611
PspPI GGNCC 3 cut(s) 1071, 1118, 1433
PstNI CAGNNNCTG 2 cut(s) 1115, 1288
PsuI RGATCY 1 cut(s) 1340
PvuII CAGCTG 1 cut(s) 449
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
RseI CAYNNNNRTG 3 cut(s) 503, 809, 878
SacI GAGCTC 2 cut(s) 29, 350
SaqAI TTAA 6 cut(s) 540, 582, 978, 1046, 1374, 1400
SatI GCNGC 3 cut(s) 255, 267, 270
Sau3AI GATC 5 cut(s) 99, 512, 642, 994, 1340
Sau96I GGNCC 3 cut(s) 1071, 1118, 1433
SchI GAGTC 1 cut(s) 1523
ScrFI CCNGG 2 cut(s) 201, 650
SduI GDGCHC 5 cut(s) 29, 350, 739, 1394, 1598
SfaNI GCATC 4 cut(s) 286, 323, 621, 1037
SinI GGWCC 3 cut(s) 1071, 1118, 1433
SmiMI CAYNNNNRTG 3 cut(s) 503, 809, 878
SmlI CTYRAG 2 cut(s) 278, 1531
SmoI CTYRAG 2 cut(s) 278, 1531
SsiI CCGC 1 cut(s) 1038
SspMI CTAG 2 cut(s) 89, 620
SstI GAGCTC 2 cut(s) 29, 350
StyD4I CCNGG 2 cut(s) 199, 648
StyI CCWWGG 6 cut(s) 88, 501, 713, 741, 1067, 1308
TaaI ACNGT 4 cut(s) 328, 753, 1479, 1513
TaiI ACGT 1 cut(s) 564
TaqI TCGA 7 cut(s) 98, 166, 183, 956, 1009, 1540, 1603
TaqII GACCGA 1 cut(s) 1473
TfiI GAWTC 5 cut(s) 8, 95, 484, 670, 1465
Tru1I TTAA 6 cut(s) 540, 582, 978, 1046, 1374, 1400
Tru9I TTAA 6 cut(s) 540, 582, 978, 1046, 1374, 1400
TscAI CASTG 3 cut(s) 89, 1368, 1399
TseFI GTSAC 4 cut(s) 283, 1473, 1485, 1573
TseI GCWGC 3 cut(s) 254, 266, 269
Tsp45I GTSAC 4 cut(s) 283, 1473, 1485, 1573
TspDTI ATGAA 3 cut(s) 716, 816, 924
TspGWI ACGGA 5 cut(s) 343, 348, 525, 1191, 1461
TspRI CASTG 3 cut(s) 89, 1368, 1399
VpaK11BI GGWCC 3 cut(s) 1071, 1118, 1433
VspI ATTAAT 1 cut(s) 1046
XagI CCTNNNNNAGG 1 cut(s) 109
XapI RAATTY 4 cut(s) 952, 1250, 1521, 1642
XceI RCATGY 1 cut(s) 877
XcmI CCANNNNNNNNNTGG 1 cut(s) 584
XmaJI CCTAGG 1 cut(s) 88
XmiI GTMKAC 1 cut(s) 441
XspI CTAG 2 cut(s) 89, 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.