Rw4G027010

TPX2 (targeting protein for Xklp2) protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001269
Physical Location & Seq
Forward (+)
53297169 .. 53300433
3265 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G027010.1

Sequence Viewer

Length: 1674 bp
ATGGGTGACTCTTCGTGTCTAATGAGGTCGTTTTCTGGGCCTTCTGAGCGTTCCGGCGAAGTCAAAGAGGGGGGCCTCCTTCGGTGTCTAGGAGAATCAGTCTCATTCGGTAGGTATGCTTCAGAACCTCTTGATTGGGAAAAATGGTCGACATTTTCTCACAATCGGTATCTGGAAGAAGTGGAGAAGTTTTCCAAACCGGGCTCGGTTGCACAGAAGAAAGCCTACTTTGAAGCACACTACAAAAAGAAGGCTGCTGAGAAAGCAGCAGCTTTGGCTGAGGTCGCAAATACTTGTGATAGCAAAGAACCCGAGTCAGTTTCTATGCACAAAAGTTGTCAAAGCTCCTCTGTCAACATGGAGTTGGCAAATGGAGAGAGCCTGATGGTTGAGGAAAATATAGTTCCAAGTACTGAGGTGGTATACTCAGCTGTTGTGAATGACCACAATCCAGATGTTGAAGGGACTCAATTGGAAAATGCAAAGGTGGATGGAGCAGAGGCGGTCATTCAAGAAAGTGTTACTGTGGAGGATGCTATTCAGGTTGAGGTGGAGAATTCTATTCAGGATGAAATGGATACTTCGATTCAGGTTCAGATGGAGAGTTCGACTCGAGTTGAAATCTCAAACCAGTTTGAAAATGACCAAAAAGAGATTGTTAATGGTGCCACCCAAGAAGAGAAGATGCCTAATAAGGAAGCTGCGAGTGAGGAAAATCTAGCTTCAACAAGCAAGAAAAGATCGGTGTACTCTTCTCCCTCAAGGTTATCTACTCAATGTAGAGCATCCAAGATCCAATCTTTTCCTGCCAAACAACTTGCTGGTTTTCCAACTGGAAAGAAGAACAATGCCACCCCAAAGAGTAAGAATTCTGAGGCTGACTTAGTTGACAAGAGGAGATCAACAAAAAAATCACTCCACATGTCAATCAATTTCAGCTCTCATGCTGGCGAAACCAGTAAAAGAACTTCGCCGGTCCTGGAGAAGATAAAAAACTTAAGAGTTGATGCTACTGCACTTAATGTGCCTGTTAAAAACTCAGCTTCCCTACAAACTGCAACTAGGGCGTCTGTTAATGGGGTATTGAAGCAATCTTCAGTAAACCCATGGTCCAAAGATAGAAGGGCAAAAACAGTACTTAATAAGTCAGTAACTGGGAGTGTAGCTGCAGATGGGGTATGCAAGTCACCCTCAAAAGATCAATCTCTTGCTGCAAATGGAAGCAAACCACGAGGTCCTATCAAGTCCTGTCCTTTTAGCCTTAGGAGTGAAGAAAGAGCAGCAAAACGGAAGGAGTTCTTTCAGAGGCTGGAAGAGAAGAAAAATGGAGAGACAGAAAGTAAGCAGCTGACAATGAGATCTAATAAGGAAAAAGCAGTGAATGATGTTAAAAAATTGCGTCAGAGTACAGGCTTAAAAGCCAAAGATCTGTCTTCTGGATCACAATTGTTAAATAATAAAGTGAAGAAGGTTCCACCGACCTGGAGTCAGTCACAGAAACTCGAGAGGCAGTCTACTACCAGCAAGGTCTTGGACCCAAGCACTCGGCCTTTGGTCAATTCTCACAGCTCTAAGCATGTCACGCAGAAATATAACCCAAGGACCAGCCAGTCAGTCACTTCCCTTCCAAAGAAGAATGCTCATGAGAATTCTTCTCCCAATATTCAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

557

Amino Acids

61.08

Weight (kDa)

9.31

Isoelectric Point (pI)

56.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 419 - 480 5.7e-12 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1609
AccB1I GGYRCC 1 cut(s) 665
AccI GTMKAC 3 cut(s) 149, 423, 1514
AciI CCGC 1 cut(s) 503
AclWI GGATC 2 cut(s) 787, 1447
AcsI RAATTY 3 cut(s) 556, 868, 1648
AcuI CTGAAG 2 cut(s) 105, 1080
AcyI GRCGYC 1 cut(s) 1067
AfaI GTAC 4 cut(s) 412, 749, 1137, 1408
AflII CTTAAG 1 cut(s) 997
AflIII ACRYGT 1 cut(s) 921
AgsI TTSAA 7 cut(s) 233, 461, 512, 620, 638, 726, 1087
AhdI GACNNNNNGTC 1 cut(s) 1485
AjnI CCWGG 2 cut(s) 978, 1481
Alw26I GTCTC 2 cut(s) 106, 1325
AlwI GGATC 2 cut(s) 787, 1447
AlwNI CAGNNNCTG 2 cut(s) 1154, 1309
Ama87I CYCGRG 3 cut(s) 311, 612, 1502
AoxI GGCC 3 cut(s) 38, 73, 1547
ApeKI GCWGC 8 cut(s) 254, 266, 269, 701, 1166, 1211, 1280, 1345
ApoI RAATTY 3 cut(s) 556, 868, 1648
Asp700I GAANNNNTTC 1 cut(s) 1295
AspS9I GGNCC 7 cut(s) 38, 73, 976, 1110, 1235, 1534, 1602
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 2 cut(s) 17, 1179
AvaI CYCGRG 3 cut(s) 311, 612, 1502
AvaII GGWCC 5 cut(s) 976, 1110, 1235, 1534, 1602
AxyI CCTNAGG 1 cut(s) 1262
BanI GGYRCC 1 cut(s) 665
BanII GRGCYC 1 cut(s) 206
BarI GAAGNNNNNNTAC 6 cut(s) 103, 135, 209, 241, 952, 984
BauI CACGAG 1 cut(s) 1230
BbsI GAAGAC 1 cut(s) 1425
BbvCI CCTCAGC 1 cut(s) 279
BbvI GCAGC 8 cut(s) 241, 278, 281, 688, 1153, 1198, 1292, 1357
BccI CCATC 4 cut(s) 379, 485, 592, 1166
BciT130I CCWGG 2 cut(s) 980, 1483
BciVI GTATCC 1 cut(s) 571
BcnI CCSGG 1 cut(s) 201
BcoDI GTCTC 2 cut(s) 106, 1325
BfaI CTAG 3 cut(s) 89, 719, 1062
BfmI CTRYAG 1 cut(s) 1167
BfrI CTTAAG 1 cut(s) 997
BfuI GTATCC 1 cut(s) 571
BglII AGATCT 2 cut(s) 1358, 1426
BisI GCNGC 8 cut(s) 255, 267, 270, 702, 1167, 1212, 1281, 1346
BlsI GCNGC 8 cut(s) 256, 268, 271, 703, 1168, 1213, 1282, 1347
BmcAI AGTACT 2 cut(s) 412, 1137
Bme1390I CCNGG 3 cut(s) 201, 980, 1483
Bme18I GGWCC 5 cut(s) 976, 1110, 1235, 1534, 1602
BmeRI GACNNNNNGTC 1 cut(s) 1485
BmeT110I CYCGRG 3 cut(s) 311, 612, 1502
BmgT120I GGNCC 7 cut(s) 38, 73, 976, 1110, 1235, 1534, 1602
BmiI GGNNCC 4 cut(s) 74, 667, 1473, 1536
BmrFI CCNGG 3 cut(s) 201, 980, 1483
BmrI ACTGGG 1 cut(s) 1164
BmsI GCATC 4 cut(s) 523, 675, 794, 997
BmuI ACTGGG 1 cut(s) 1164
BpiI GAAGAC 1 cut(s) 1425
BplI GAGNNNNNCTC 2 cut(s) 595, 627
BpmI CTGGAG 2 cut(s) 1001, 1504
Bpu10I CCTNAGC 1 cut(s) 279
BpuEI CTTGAG 1 cut(s) 745
BpuMI CCSGG 1 cut(s) 201
BsaHI GRCGYC 1 cut(s) 1067
BsaJI CCNNGG 2 cut(s) 1106, 1598
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bse118I RCCGGY 1 cut(s) 973
Bse1I ACTGG 5 cut(s) 631, 838, 957, 1159, 1609
Bse21I CCTNAGG 1 cut(s) 1262
BseBI CCWGG 2 cut(s) 980, 1483
BseDI CCNNGG 2 cut(s) 1106, 1598
BseGI GGATG 4 cut(s) 496, 538, 574, 785
BseMII CTCAG 7 cut(s) 36, 249, 270, 405, 441, 864, 1053
BseNI ACTGG 5 cut(s) 631, 838, 957, 1159, 1609
BseRI GAGGAG 2 cut(s) 337, 910
BseXI GCAGC 8 cut(s) 241, 278, 281, 688, 1153, 1198, 1292, 1357
BsgI GTGCAG 1 cut(s) 999
BshFI GGCC 3 cut(s) 40, 75, 1549
BshNI GGYRCC 1 cut(s) 665
BsiHKCI CYCGRG 3 cut(s) 311, 612, 1502
BsiSI CCGG 3 cut(s) 54, 200, 974
BslFI GGGAC 1 cut(s) 478
BsmAI GTCTC 2 cut(s) 106, 1325
BsmFI GGGAC 1 cut(s) 478
BsmI GAATGC 1 cut(s) 1642
BsnI GGCC 3 cut(s) 40, 75, 1549
BsoBI CYCGRG 3 cut(s) 311, 612, 1502
Bsp1286I GDGCHC 1 cut(s) 206
Bsp143I GATC 7 cut(s) 740, 792, 899, 1198, 1358, 1426, 1439
Bsp19I CCATGG 1 cut(s) 1106
BspACI CCGC 1 cut(s) 503
BspANI GGCC 3 cut(s) 40, 75, 1549
BspCNI CTCAG 7 cut(s) 37, 250, 271, 406, 440, 865, 1052
BspHI TCATGA 1 cut(s) 1642
BspLI GGNNCC 4 cut(s) 74, 667, 1473, 1536
BspMAI CTGCAG 1 cut(s) 1171
BspPI GGATC 2 cut(s) 787, 1447
BspT107I GGYRCC 1 cut(s) 665
BspTI CTTAAG 1 cut(s) 997
BsrFI RCCGGY 1 cut(s) 973
BsrI ACTGG 5 cut(s) 631, 838, 957, 1159, 1609
BssAI RCCGGY 1 cut(s) 973
BssECI CCNNGG 2 cut(s) 1106, 1598
BssMI GATC 7 cut(s) 740, 792, 899, 1198, 1358, 1426, 1439
BssNAI GTATAC 1 cut(s) 424
BssNI GRCGYC 1 cut(s) 1067
BssSI CACGAG 1 cut(s) 1230
BssT1I CCWWGG 2 cut(s) 1106, 1598
Bst1107I GTATAC 1 cut(s) 424
Bst2BI CACGAG 1 cut(s) 1230
Bst2UI CCWGG 2 cut(s) 980, 1483
Bst4CI ACNGT 2 cut(s) 526, 1135
Bst6I CTCTTC 4 cut(s) 16, 672, 757, 1308
BstACI GRCGYC 1 cut(s) 1067
BstAFI CTTAAG 1 cut(s) 997
BstC8I GCNNGC 1 cut(s) 949
BstDSI CCRYGG 1 cut(s) 1106
BstF5I GGATG 4 cut(s) 496, 538, 574, 785
BstKTI GATC 7 cut(s) 743, 795, 902, 1201, 1361, 1429, 1442
BstMAI GTCTC 2 cut(s) 106, 1325
BstMBI GATC 7 cut(s) 740, 792, 899, 1198, 1358, 1426, 1439
BstMWI GCNNNNNNNGC 6 cut(s) 46, 263, 275, 284, 1064, 1582
BstNI CCWGG 2 cut(s) 980, 1483
BstNSI RCATGY 2 cut(s) 925, 1580
BstSCI CCNGG 3 cut(s) 199, 978, 1481
BstSFI CTRYAG 1 cut(s) 1167
BstV1I GCAGC 8 cut(s) 241, 278, 281, 688, 1153, 1198, 1292, 1357
BstV2I GAAGAC 1 cut(s) 1425
BstX2I RGATCY 3 cut(s) 792, 1358, 1426
BstXI CCANNNNNNTGG 1 cut(s) 1482
BstYI RGATCY 3 cut(s) 792, 1358, 1426
BstZ17I GTATAC 1 cut(s) 424
Bsu36I CCTNAGG 1 cut(s) 1262
BsuI GTATCC 1 cut(s) 571
BsuRI GGCC 3 cut(s) 40, 75, 1549
BtgI CCRYGG 1 cut(s) 1106
BtsCI GGATG 4 cut(s) 496, 538, 574, 785
BtsI GCAGTG 1 cut(s) 1383
BtsIMutI CAGTG 1 cut(s) 1383
Cac8I GCNNGC 1 cut(s) 949
CaiI CAGNNNCTG 2 cut(s) 1154, 1309
CciI TCATGA 1 cut(s) 1642
Cfr10I RCCGGY 1 cut(s) 973
Cfr13I GGNCC 7 cut(s) 38, 73, 976, 1110, 1235, 1534, 1602
CseI GACGC 2 cut(s) 1056, 1388
Csp6I GTAC 4 cut(s) 411, 748, 1136, 1407
CviAII CATG 6 cut(s) 358, 922, 944, 1107, 1577, 1643
CviQI GTAC 4 cut(s) 411, 748, 1136, 1407
DpnI GATC 7 cut(s) 742, 794, 901, 1200, 1360, 1428, 1441
DpnII GATC 7 cut(s) 740, 792, 899, 1198, 1358, 1426, 1439
DrdI GACNNNNNNGTC 1 cut(s) 1609
DriI GACNNNNNGTC 1 cut(s) 1485
DseDI GACNNNNNNGTC 1 cut(s) 1609
Eam1104I CTCTTC 4 cut(s) 16, 672, 757, 1308
Eam1105I GACNNNNNGTC 1 cut(s) 1485
EarI CTCTTC 4 cut(s) 16, 672, 757, 1308
Eco130I CCWWGG 2 cut(s) 1106, 1598
Eco24I GRGCYC 1 cut(s) 206
Eco47I GGWCC 5 cut(s) 976, 1110, 1235, 1534, 1602
Eco57I CTGAAG 2 cut(s) 105, 1080
Eco81I CCTNAGG 1 cut(s) 1262
Eco88I CYCGRG 3 cut(s) 311, 612, 1502
EcoO109I RGGNCCY 2 cut(s) 73, 1235
EcoRI GAATTC 3 cut(s) 556, 868, 1648
EcoRII CCWGG 2 cut(s) 978, 1481
EcoT14I CCWWGG 2 cut(s) 1106, 1598
EcoT38I GRGCYC 1 cut(s) 206
ErhI CCWWGG 2 cut(s) 1106, 1598
FaeI CATG 6 cut(s) 361, 925, 947, 1110, 1580, 1646
FalI AAGNNNNNCTT 2 cut(s) 1283, 1315
FaqI GGGAC 1 cut(s) 478
FatI CATG 6 cut(s) 357, 921, 943, 1106, 1576, 1642
FblI GTMKAC 3 cut(s) 149, 423, 1514
Fnu4HI GCNGC 8 cut(s) 255, 267, 270, 702, 1167, 1212, 1281, 1346
FokI GGATG 4 cut(s) 503, 545, 581, 772
FriOI GRGCYC 1 cut(s) 206
Fsp4HI GCNGC 8 cut(s) 255, 267, 270, 702, 1167, 1212, 1281, 1346
FspBI CTAG 3 cut(s) 89, 719, 1062
GluI GCNGC 8 cut(s) 255, 267, 270, 702, 1167, 1212, 1281, 1346
GsuI CTGGAG 2 cut(s) 1001, 1504
HaeIII GGCC 3 cut(s) 40, 75, 1549
HapII CCGG 3 cut(s) 54, 200, 974
HgaI GACGC 2 cut(s) 1056, 1388
Hin1I GRCGYC 1 cut(s) 1067
Hin1II CATG 6 cut(s) 361, 925, 947, 1110, 1580, 1646
HincII GTYRAC 3 cut(s) 150, 355, 889
HindII GTYRAC 3 cut(s) 150, 355, 889
HinfI GANTC 7 cut(s) 8, 95, 314, 466, 586, 610, 1486
HpaII CCGG 3 cut(s) 54, 200, 974
HphI GGTGA 2 cut(s) 17, 1179
Hpy166II GTNNAC 7 cut(s) 150, 355, 424, 748, 889, 1102, 1515
Hpy188I TCNGA 7 cut(s) 46, 124, 597, 874, 1305, 1404, 1668
Hpy188III TCNNGA 8 cut(s) 131, 173, 452, 512, 566, 1437, 1504, 1643
Hpy8I GTNNAC 7 cut(s) 150, 355, 424, 748, 889, 1102, 1515
HpyAV CCTTC 8 cut(s) 51, 89, 244, 455, 1116, 1285, 1462, 1634
HpyCH4III ACNGT 2 cut(s) 526, 1135
HpyCH4V TGCA 8 cut(s) 212, 328, 482, 1016, 1058, 1169, 1182, 1214
HpyF10VI GCNNNNNNNGC 6 cut(s) 46, 263, 275, 284, 1064, 1582
Hsp92I GRCGYC 1 cut(s) 1067
Hsp92II CATG 6 cut(s) 361, 925, 947, 1110, 1580, 1646
Kzo9I GATC 7 cut(s) 740, 792, 899, 1198, 1358, 1426, 1439
LmnI GCTCC 2 cut(s) 350, 494
Lsp1109I GCAGC 8 cut(s) 241, 278, 281, 688, 1153, 1198, 1292, 1357
LweI GCATC 4 cut(s) 523, 675, 794, 997
MaeI CTAG 3 cut(s) 89, 719, 1062
MaeIII GTNAC 7 cut(s) 5, 520, 1150, 1185, 1491, 1579, 1615
MalI GATC 7 cut(s) 742, 794, 901, 1200, 1360, 1428, 1441
MboI GATC 7 cut(s) 740, 792, 899, 1198, 1358, 1426, 1439
MfeI CAATTG 2 cut(s) 470, 1445
MflI RGATCY 3 cut(s) 792, 1358, 1426
MhlI GDGCHC 1 cut(s) 206
MluCI AATT 8 cut(s) 470, 556, 868, 931, 1394, 1445, 1558, 1648
MlyI GAGTC 5 cut(s) 2, 323, 460, 604, 1495
MmeI TCCRAC 1 cut(s) 854
MroXI GAANNNNTTC 1 cut(s) 1295
MseI TTAA 9 cut(s) 660, 998, 1020, 1032, 1074, 1140, 1389, 1415, 1451
MspA1I CMGCKG 2 cut(s) 431, 1348
MspCI CTTAAG 1 cut(s) 997
MspI CCGG 3 cut(s) 54, 200, 974
MspR9I CCNGG 3 cut(s) 201, 980, 1483
MunI CAATTG 2 cut(s) 470, 1445
Mva1269I GAATGC 1 cut(s) 1642
MvaI CCWGG 2 cut(s) 980, 1483
MwoI GCNNNNNNNGC 6 cut(s) 46, 263, 275, 284, 1064, 1582
NciI CCSGG 1 cut(s) 201
NcoI CCATGG 1 cut(s) 1106
NdeII GATC 7 cut(s) 740, 792, 899, 1198, 1358, 1426, 1439
NlaIII CATG 6 cut(s) 361, 925, 947, 1110, 1580, 1646
NlaIV GGNNCC 4 cut(s) 74, 667, 1473, 1536
NmeAIII GCCGAG 1 cut(s) 1525
NmuCI GTSAC 5 cut(s) 5, 1185, 1491, 1579, 1615
NspI RCATGY 2 cut(s) 925, 1580
PaeR7I CTCGAG 2 cut(s) 612, 1502
PagI TCATGA 1 cut(s) 1642
PciI ACATGT 1 cut(s) 921
PctI GAATGC 1 cut(s) 1642
PdmI GAANNNNTTC 1 cut(s) 1295
PfeI GAWTC 2 cut(s) 95, 586
PfoI TCCNGGA 1 cut(s) 978
PkrI GCNGC 8 cut(s) 256, 268, 271, 703, 1168, 1213, 1282, 1347
PleI GAGTC 5 cut(s) 2, 322, 460, 604, 1494
PpsI GAGTC 5 cut(s) 2, 322, 460, 604, 1494
PpuMI RGGWCCY 1 cut(s) 1235
PscI ACATGT 1 cut(s) 921
Psp5II RGGWCCY 1 cut(s) 1235
Psp6I CCWGG 2 cut(s) 978, 1481
PspGI CCWGG 2 cut(s) 978, 1481
PspN4I GGNNCC 4 cut(s) 74, 667, 1473, 1536
PspPI GGNCC 7 cut(s) 38, 73, 976, 1110, 1235, 1534, 1602
PspPPI RGGWCCY 1 cut(s) 1235
PspXI VCTCGAGB 1 cut(s) 612
PstI CTGCAG 1 cut(s) 1171
PstNI CAGNNNCTG 2 cut(s) 1154, 1309
PsuI RGATCY 3 cut(s) 792, 1358, 1426
PvuII CAGCTG 2 cut(s) 431, 1348
RsaI GTAC 4 cut(s) 412, 749, 1137, 1408
RsaNI GTAC 4 cut(s) 411, 748, 1136, 1407
SalI GTCGAC 1 cut(s) 148
SaqAI TTAA 9 cut(s) 660, 998, 1020, 1032, 1074, 1140, 1389, 1415, 1451
SatI GCNGC 8 cut(s) 255, 267, 270, 702, 1167, 1212, 1281, 1346
Sau3AI GATC 7 cut(s) 740, 792, 899, 1198, 1358, 1426, 1439
Sau96I GGNCC 7 cut(s) 38, 73, 976, 1110, 1235, 1534, 1602
ScaI AGTACT 2 cut(s) 412, 1137
SchI GAGTC 5 cut(s) 2, 323, 460, 604, 1495
ScrFI CCNGG 3 cut(s) 201, 980, 1483
SduI GDGCHC 1 cut(s) 206
SfaNI GCATC 4 cut(s) 523, 675, 794, 997
SfcI CTRYAG 1 cut(s) 1167
Sfr274I CTCGAG 2 cut(s) 612, 1502
SinI GGWCC 5 cut(s) 976, 1110, 1235, 1534, 1602
SlaI CTCGAG 2 cut(s) 612, 1502
SmlI CTYRAG 4 cut(s) 612, 760, 997, 1502
SmoI CTYRAG 4 cut(s) 612, 760, 997, 1502
Sse9I AATT 8 cut(s) 470, 556, 868, 931, 1394, 1445, 1558, 1648
SsiI CCGC 1 cut(s) 503
SspI AATATT 1 cut(s) 1663
SspMI CTAG 3 cut(s) 89, 719, 1062
StyD4I CCNGG 3 cut(s) 199, 978, 1481
StyI CCWWGG 2 cut(s) 1106, 1598
TaaI ACNGT 2 cut(s) 526, 1135
TaqI TCGA 5 cut(s) 149, 584, 608, 613, 1503
TasI AATT 8 cut(s) 470, 556, 868, 931, 1394, 1445, 1558, 1648
TatI WGTACW 4 cut(s) 410, 747, 1135, 1406
TfiI GAWTC 2 cut(s) 95, 586
Tru1I TTAA 9 cut(s) 660, 998, 1020, 1032, 1074, 1140, 1389, 1415, 1451
Tru9I TTAA 9 cut(s) 660, 998, 1020, 1032, 1074, 1140, 1389, 1415, 1451
TscAI CASTG 1 cut(s) 1383
TseFI GTSAC 5 cut(s) 5, 1185, 1491, 1579, 1615
TseI GCWGC 8 cut(s) 254, 266, 269, 701, 1166, 1211, 1280, 1345
Tsp45I GTSAC 5 cut(s) 5, 1185, 1491, 1579, 1615
TspDTI ATGAA 1 cut(s) 585
TspGWI ACGGA 1 cut(s) 1303
TspRI CASTG 1 cut(s) 1383
Vha464I CTTAAG 1 cut(s) 997
VpaK11BI GGWCC 5 cut(s) 976, 1110, 1235, 1534, 1602
XapI RAATTY 3 cut(s) 556, 868, 1648
XceI RCATGY 2 cut(s) 925, 1580
XcmI CCANNNNNNNNNTGG 1 cut(s) 1528
XhoI CTCGAG 2 cut(s) 612, 1502
XmiI GTMKAC 3 cut(s) 149, 423, 1514
XmnI GAANNNNTTC 1 cut(s) 1295
XspI CTAG 3 cut(s) 89, 719, 1062
ZrmI AGTACT 2 cut(s) 412, 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.