pycom17g01580

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001269
Physical Location & Seq
Forward (+)
1045287 .. 1047938
2652 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g01580.5

Sequence Viewer

Length: 1698 bp
ATGGGAGAATCGACGTGTCTGATGCAGCCGTTCTCCTATGCTGCCGGCATACCCAACGACGCACACGAGGGGAACCCGATTCATGCTCTTGGGCAGTCAGTGTCGTTCGGGAGGTTCGTGTCGGAGTCATTGGCTTGGGAGAAATGGTCGACATTTTCGCACAACCGGTATGTGGAGGAGGCCGAGAGGTACGCCCGCCCCGGTTCAGTTGCGCAGAAAAAGGCTTTCTTTGAAGCACATTACAAGAAAATCGCTGCTCAAAGAGCAGCCGCCTTGCTTGAACAAGCAAATGCTAATGCTAATGCTGCTGCTGCATCGGAAAATGCACCAGAACCCAAATCTGAAAGCCGGGATCTAAAGACGAGGATTTCAACCTCTGAAGTGTTCGTCGATGAGCAAAAGGAAGAGAAAGTCGAACAGAAGGTCTACGAGCCAAATCGAGAAGAAGAATCTATTGGTAAATATACCAATGACTATAATGCAAACACTGAAATGGAGAAATTTGAAAGCAGTAAGGTGCACCCTGTAGATCATGAAGACCAAGTTTCGGGGGAGAATTCGGTTGAGGTAGAACTTTCGAGCCAATGTGCCGATAAAGACAAGGAGGTAAACGGCATGGAGCTCACCGGCGCACCCCAGATCGAAAAGCCTCTGTTAAAGAGTTACAGATCGAATCACGAAGCTTTGACACCAGTGAAAACGAAAAAAACCAGACTATTTAGTTCCTCCAAATCATCATTAGCATATCATAAAGCACCCAAAGTCCCATCTTCTCCAGCCAAACCCACAGCTCCTAGTTGTTCCCGAAAGGATGACATTATCACTCCATTGCGTAAATATCCGGCTGCAGAACTGGAGGACAAGAAGAAATCAACTCCGAAATCACTTCACAAGTCGGTCAAATTTACGCCCATCAGGGAGCTTTCTAGACTGACTTCGACCGTCATGAGGAAGATTGAAAACTCAAGAGTTGGTGCTAGTTCTTCCAAGACATCAAAAGAATGCCTGACTCCTCTGAAGACTCCAACTACGGTATCTAGGAATGAGGTGCGCAAACGTTCTACAACCACCCCGTGTTCAGAAAAGCGAAGGGCCAAAACACCGCTTGATCCCTCAGCCAGTGGAACCAAAACTCCTGTGTCCAAATGGCGTTTACTCCGTACAGATTGTTCAAAGTTTTTGAGTGCCTGCAGAAACAAGGCGAGGTCACCATTTTCATCGGCCCCTATCGCTTTAAGAACTGAAGAAAGAGCTGCAAGTAGAAAGAAGAAGCTCGAAGAAAAGTTTAATGCGAATGAGGAACGAAAGGTGCAGGTGCAAAGAAAACTCAAGGAGGAAGAAGAAACAGAAATTGGAAAGTACCGCCAAACCCTTTGCTTCAAGGCGAGGCCGCTTCCTGATTTTTATAAAGAAAGAAAATCACCAAGGAACGAGATACATAAGGTTCCAGTTACTCGTCAGTCAAAGAACCCAGGGAAAAAGCCCACTCCAAGCACAGTTGAGAGCTCAACGTCTCTACCTCCTAGTGGAGCTTCAATCAAGAGCATTGGCTCCAAGAATGTCCAAGGAAGGAATGACCGAACCCCAACCTGTTCTCTCATCTCACGATCCTTCAAGACCACTCGCGAGAATACATCTCCAAATATTCAGCTTGGACAACCTAAGCTTGAAAACAAGTTTCTGTTGGAGCAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

566

Amino Acids

63.25

Weight (kDa)

9.65

Isoelectric Point (pI)

52.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1407
AarI CACCTGC 1 cut(s) 1303
Acc16I TGCGCA 2 cut(s) 213, 1052
Acc36I ACCTGC 1 cut(s) 1303
AccI GTMKAC 2 cut(s) 149, 426
AccII CGCG 1 cut(s) 1626
AciI CCGC 5 cut(s) 196, 270, 1103, 1363, 1391
AclI AACGTT 1 cut(s) 1057
AclWI GGATC 3 cut(s) 360, 1103, 1602
AcsI RAATTY 3 cut(s) 500, 556, 902
AcuI CTGAAG 3 cut(s) 399, 1037, 1263
AdeI CACNNNGTG 1 cut(s) 1074
AfaI GTAC 3 cut(s) 191, 1162, 1361
AfiI CCNNNNNNNGG 4 cut(s) 172, 547, 948, 1526
AflIII ACRYGT 1 cut(s) 14
AgeI ACCGGT 1 cut(s) 165
AjiI CACGTC 1 cut(s) 15
AjnI CCWGG 1 cut(s) 1471
AjuI GAANNNNNNNTTGG 4 cut(s) 438, 470, 1335, 1367
AloI GAACNNNNNNTCC 2 cut(s) 1117, 1149
Alw21I GWGCWC 3 cut(s) 522, 624, 1508
Alw26I GTCTC 1 cut(s) 1518
Alw44I GTGCAC 1 cut(s) 518
AlwI GGATC 3 cut(s) 360, 1103, 1602
AoxI GGCC 4 cut(s) 180, 1092, 1221, 1388
ApaLI GTGCAC 1 cut(s) 518
ApeKI GCWGC 9 cut(s) 25, 41, 254, 266, 305, 308, 311, 845, 1253
ApoI RAATTY 3 cut(s) 500, 556, 902
ArsI GACNNNNNNTTYG 2 cut(s) 408, 440
AsiGI ACCGGT 1 cut(s) 165
AspLEI GCGC 3 cut(s) 214, 632, 1053
AspS9I GGNCC 2 cut(s) 1092, 1222
AsuC2I CCSGG 2 cut(s) 201, 350
AsuHPI GGTGA 3 cut(s) 616, 1200, 1413
BaeGI GKGCMC 1 cut(s) 522
BaeI ACNNNNGTAYC 2 cut(s) 1017, 1050
BanII GRGCYC 2 cut(s) 624, 1508
BauI CACGAG 1 cut(s) 65
BbsI GAAGAC 2 cut(s) 543, 1025
Bbv12I GWGCWC 3 cut(s) 522, 624, 1508
BbvCI CCTCAGC 1 cut(s) 1114
BbvI GCAGC 9 cut(s) 28, 37, 241, 278, 292, 295, 298, 832, 1240
BccI CCATC 2 cut(s) 775, 920
BceAI ACGGC 2 cut(s) 13, 628
BciT130I CCWGG 1 cut(s) 1473
BcnI CCSGG 2 cut(s) 201, 350
BcoDI GTCTC 1 cut(s) 1518
BfaI CTAG 5 cut(s) 795, 927, 978, 1038, 1524
BfmI CTRYAG 3 cut(s) 525, 846, 1189
BfuAI ACCTGC 1 cut(s) 1303
Bme1390I CCNGG 3 cut(s) 201, 350, 1473
BmgBI CACGTC 1 cut(s) 15
BmgT120I GGNCC 2 cut(s) 1092, 1222
BmiI GGNNCC 5 cut(s) 74, 1126, 1224, 1446, 1552
BmrFI CCNGG 3 cut(s) 201, 350, 1473
BmsI GCATC 2 cut(s) 12, 323
BpiI GAAGAC 2 cut(s) 543, 1025
BpmI CTGGAG 2 cut(s) 759, 875
Bpu10I CCTNAGC 2 cut(s) 1114, 1662
BpuEI CTTGAG 2 cut(s) 949, 1313
BpuMI CCSGG 2 cut(s) 201, 350
BsaJI CCNNGG 5 cut(s) 199, 1424, 1471, 1472, 1564
BsaWI WCCGGW 1 cut(s) 165
BsaXI ACNNNNNCTCC 5 cut(s) 131, 161, 1117, 1147, 1679
Bsc4I CCNNNNNNNGG 4 cut(s) 172, 547, 948, 1526
Bse118I RCCGGY 3 cut(s) 44, 165, 626
Bse1I ACTGG 4 cut(s) 692, 858, 1119, 1448
Bse3DI GCAATG 1 cut(s) 827
BseBI CCWGG 1 cut(s) 1473
BseDI CCNNGG 5 cut(s) 199, 1424, 1471, 1472, 1564
BseGI GGATG 1 cut(s) 817
BseLI CCNNNNNNNGG 4 cut(s) 172, 547, 948, 1526
BseMI GCAATG 1 cut(s) 827
BseMII CTCAG 1 cut(s) 1128
BseNI ACTGG 4 cut(s) 692, 858, 1119, 1448
BseRI GAGGAG 2 cut(s) 191, 1002
BseSI GKGCMC 1 cut(s) 522
BseXI GCAGC 9 cut(s) 28, 37, 241, 278, 292, 295, 298, 832, 1240
BsgI GTGCAG 1 cut(s) 1331
Bsh1236I CGCG 1 cut(s) 1626
Bsh1285I CGRYCG 1 cut(s) 942
BshFI GGCC 4 cut(s) 182, 1094, 1223, 1390
BshTI ACCGGT 1 cut(s) 165
BsiEI CGRYCG 1 cut(s) 942
BsiHKAI GWGCWC 3 cut(s) 522, 624, 1508
BsiSI CCGG 6 cut(s) 45, 166, 201, 349, 627, 842
BslFI GGGAC 1 cut(s) 749
BslI CCNNNNNNNGG 4 cut(s) 172, 547, 948, 1526
BsmAI GTCTC 1 cut(s) 1518
BsmBI CGTCTC 1 cut(s) 1518
BsmFI GGGAC 1 cut(s) 749
BsmI GAATGC 1 cut(s) 1007
BsnI GGCC 4 cut(s) 182, 1094, 1223, 1390
Bsp1286I GDGCHC 3 cut(s) 522, 624, 1508
Bsp143I GATC 6 cut(s) 352, 529, 639, 668, 1108, 1607
Bsp68I TCGCGA 1 cut(s) 1626
BspACI CCGC 5 cut(s) 196, 270, 1103, 1363, 1391
BspANI GGCC 4 cut(s) 182, 1094, 1223, 1390
BspCNI CTCAG 1 cut(s) 1127
BspFNI CGCG 1 cut(s) 1626
BspHI TCATGA 2 cut(s) 532, 945
BspLI GGNNCC 5 cut(s) 74, 1126, 1224, 1446, 1552
BspMAI CTGCAG 2 cut(s) 850, 1193
BspMI ACCTGC 1 cut(s) 1303
BspPI GGATC 3 cut(s) 360, 1103, 1602
BsrDI GCAATG 1 cut(s) 827
BsrFI RCCGGY 3 cut(s) 44, 165, 626
BsrI ACTGG 4 cut(s) 692, 858, 1119, 1448
BssAI RCCGGY 3 cut(s) 44, 165, 626
BssECI CCNNGG 5 cut(s) 199, 1424, 1471, 1472, 1564
BssMI GATC 6 cut(s) 352, 529, 639, 668, 1108, 1607
BssSI CACGAG 1 cut(s) 65
BssT1I CCWWGG 2 cut(s) 1424, 1564
Bst2BI CACGAG 1 cut(s) 65
Bst2UI CCWGG 1 cut(s) 1473
Bst4CI ACNGT 3 cut(s) 943, 1033, 1498
Bst6I CTCTTC 1 cut(s) 399
BstC8I GCNNGC 3 cut(s) 46, 196, 1189
BstDEI CTNAG 2 cut(s) 1114, 1662
BstEII GGTNACC 1 cut(s) 1206
BstF5I GGATG 1 cut(s) 817
BstFNI CGCG 1 cut(s) 1626
BstHHI GCGC 3 cut(s) 214, 632, 1053
BstKTI GATC 6 cut(s) 355, 532, 642, 671, 1111, 1610
BstMAI GTCTC 1 cut(s) 1518
BstMBI GATC 6 cut(s) 352, 529, 639, 668, 1108, 1607
BstMCI CGRYCG 1 cut(s) 942
BstMWI GCNNNNNNNGC 4 cut(s) 263, 305, 311, 1229
BstNI CCWGG 1 cut(s) 1473
BstPI GGTNACC 1 cut(s) 1206
BstSCI CCNGG 3 cut(s) 199, 348, 1471
BstSFI CTRYAG 3 cut(s) 525, 846, 1189
BstSLI GKGCMC 1 cut(s) 522
BstUI CGCG 1 cut(s) 1626
BstV1I GCAGC 9 cut(s) 28, 37, 241, 278, 292, 295, 298, 832, 1240
BstV2I GAAGAC 2 cut(s) 543, 1025
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BsuRI GGCC 4 cut(s) 182, 1094, 1223, 1390
BtrI CACGTC 1 cut(s) 15
BtsCI GGATG 1 cut(s) 817
BtsIMutI CAGTG 4 cut(s) 105, 486, 699, 1126
BtuMI TCGCGA 1 cut(s) 1626
BveI ACCTGC 1 cut(s) 1303
Cac8I GCNNGC 3 cut(s) 46, 196, 1189
CciI TCATGA 2 cut(s) 532, 945
CfoI GCGC 3 cut(s) 214, 632, 1053
Cfr10I RCCGGY 3 cut(s) 44, 165, 626
Cfr13I GGNCC 2 cut(s) 1092, 1222
CseI GACGC 1 cut(s) 68
Csp6I GTAC 3 cut(s) 190, 1161, 1360
CspAI ACCGGT 1 cut(s) 165
CviAII CATG 4 cut(s) 83, 533, 616, 946
CviQI GTAC 3 cut(s) 190, 1161, 1360
DdeI CTNAG 2 cut(s) 1114, 1662
DpnI GATC 6 cut(s) 354, 531, 641, 670, 1110, 1609
DpnII GATC 6 cut(s) 352, 529, 639, 668, 1108, 1607
DraIII CACNNNGTG 1 cut(s) 1074
Eam1104I CTCTTC 1 cut(s) 399
EarI CTCTTC 1 cut(s) 399
Ecl136II GAGCTC 2 cut(s) 622, 1506
Eco130I CCWWGG 2 cut(s) 1424, 1564
Eco24I GRGCYC 2 cut(s) 624, 1508
Eco53kI GAGCTC 2 cut(s) 622, 1506
Eco57I CTGAAG 3 cut(s) 399, 1037, 1263
Eco91I GGTNACC 1 cut(s) 1206
EcoICRI GAGCTC 2 cut(s) 622, 1506
EcoO65I GGTNACC 1 cut(s) 1206
EcoRI GAATTC 1 cut(s) 556
EcoRII CCWGG 1 cut(s) 1471
EcoT14I CCWWGG 2 cut(s) 1424, 1564
EcoT38I GRGCYC 2 cut(s) 624, 1508
ErhI CCWWGG 2 cut(s) 1424, 1564
Esp3I CGTCTC 1 cut(s) 1518
FaeI CATG 4 cut(s) 86, 536, 619, 949
FalI AAGNNNNNCTT 2 cut(s) 212, 244
FaqI GGGAC 1 cut(s) 749
FatI CATG 4 cut(s) 82, 532, 615, 945
FauI CCCGC 1 cut(s) 203
FblI GTMKAC 2 cut(s) 149, 426
FokI GGATG 1 cut(s) 824
FriOI GRGCYC 2 cut(s) 624, 1508
FspBI CTAG 5 cut(s) 795, 927, 978, 1038, 1524
FspI TGCGCA 2 cut(s) 213, 1052
GlaI GCGC 3 cut(s) 213, 631, 1052
GsuI CTGGAG 2 cut(s) 759, 875
HaeIII GGCC 4 cut(s) 182, 1094, 1223, 1390
HapII CCGG 6 cut(s) 45, 166, 201, 349, 627, 842
HgaI GACGC 1 cut(s) 68
HhaI GCGC 3 cut(s) 214, 632, 1053
Hin1II CATG 4 cut(s) 86, 536, 619, 949
Hin6I GCGC 3 cut(s) 212, 630, 1051
HinP1I GCGC 3 cut(s) 212, 630, 1051
HincII GTYRAC 1 cut(s) 150
HindII GTYRAC 1 cut(s) 150
HindIII AAGCTT 2 cut(s) 681, 1664
HinfI GANTC 7 cut(s) 8, 79, 125, 449, 673, 1009, 1021
HpaII CCGG 6 cut(s) 45, 166, 201, 349, 627, 842
HphI GGTGA 3 cut(s) 616, 1200, 1413
Hpy166II GTNNAC 5 cut(s) 150, 427, 520, 610, 1154
Hpy188I TCNGA 8 cut(s) 21, 124, 319, 343, 379, 879, 1017, 1081
Hpy8I GTNNAC 5 cut(s) 150, 427, 520, 610, 1154
Hpy99I CGWCG 3 cut(s) 16, 62, 392
HpyAV CCTTC 4 cut(s) 415, 1083, 1563, 1621
HpyCH4III ACNGT 3 cut(s) 943, 1033, 1498
HpyCH4IV ACGT 3 cut(s) 14, 1057, 1511
HpyF10VI GCNNNNNNNGC 4 cut(s) 263, 305, 311, 1229
HpyF3I CTNAG 2 cut(s) 1114, 1662
HpySE526I ACGT 3 cut(s) 14, 1057, 1511
Hsp92II CATG 4 cut(s) 86, 536, 619, 949
HspAI GCGC 3 cut(s) 212, 630, 1051
KroI GCCGGC 1 cut(s) 44
KroNI GCCGGC 1 cut(s) 46
Kzo9I GATC 6 cut(s) 352, 529, 639, 668, 1108, 1607
LmnI GCTCC 6 cut(s) 619, 796, 919, 1529, 1556, 1687
Lsp1109I GCAGC 9 cut(s) 28, 37, 241, 278, 292, 295, 298, 832, 1240
LweI GCATC 2 cut(s) 12, 323
MaeI CTAG 5 cut(s) 795, 927, 978, 1038, 1524
MaeII ACGT 3 cut(s) 14, 1057, 1511
MaeIII GTNAC 3 cut(s) 662, 1206, 1450
MalI GATC 6 cut(s) 354, 531, 641, 670, 1110, 1609
MboI GATC 6 cut(s) 352, 529, 639, 668, 1108, 1607
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 3 cut(s) 522, 624, 1508
MluCI AATT 4 cut(s) 500, 556, 902, 1350
MlyI GAGTC 3 cut(s) 134, 1003, 1015
MmeI TCCRAC 3 cut(s) 102, 1049, 1665
MroNI GCCGGC 1 cut(s) 44
MseI TTAA 3 cut(s) 656, 1235, 1287
MslI CAYNNNNRTG 1 cut(s) 491
MspI CCGG 6 cut(s) 45, 166, 201, 349, 627, 842
MspR9I CCNGG 3 cut(s) 201, 350, 1473
Mva1269I GAATGC 1 cut(s) 1007
MvaI CCWGG 1 cut(s) 1473
MvnI CGCG 1 cut(s) 1626
MwoI GCNNNNNNNGC 4 cut(s) 263, 305, 311, 1229
NaeI GCCGGC 1 cut(s) 46
NciI CCSGG 2 cut(s) 201, 350
NdeII GATC 6 cut(s) 352, 529, 639, 668, 1108, 1607
NgoMIV GCCGGC 1 cut(s) 44
NlaIII CATG 4 cut(s) 86, 536, 619, 949
NlaIV GGNNCC 5 cut(s) 74, 1126, 1224, 1446, 1552
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 1 cut(s) 1206
NruI TCGCGA 1 cut(s) 1626
NsbI TGCGCA 2 cut(s) 213, 1052
PagI TCATGA 2 cut(s) 532, 945
PaqCI CACCTGC 1 cut(s) 1303
PasI CCCWGGG 1 cut(s) 1472
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 114
PctI GAATGC 1 cut(s) 1007
PdiI GCCGGC 1 cut(s) 46
PfeI GAWTC 4 cut(s) 8, 79, 449, 673
PinAI ACCGGT 1 cut(s) 165
PleI GAGTC 3 cut(s) 133, 1003, 1015
PpsI GAGTC 3 cut(s) 133, 1003, 1015
PsiI TTATAA 1 cut(s) 1407
Psp124BI GAGCTC 2 cut(s) 624, 1508
Psp1406I AACGTT 1 cut(s) 1057
Psp6I CCWGG 1 cut(s) 1471
PspEI GGTNACC 1 cut(s) 1206
PspGI CCWGG 1 cut(s) 1471
PspN4I GGNNCC 5 cut(s) 74, 1126, 1224, 1446, 1552
PspPI GGNCC 2 cut(s) 1092, 1222
PsrI GAACNNNNNNTAC 2 cut(s) 1153, 1185
PstI CTGCAG 2 cut(s) 850, 1193
PsuI RGATCY 1 cut(s) 352
RruI TCGCGA 1 cut(s) 1626
RsaI GTAC 3 cut(s) 191, 1162, 1361
RsaNI GTAC 3 cut(s) 190, 1161, 1360
RseI CAYNNNNRTG 1 cut(s) 491
SacI GAGCTC 2 cut(s) 624, 1508
SalI GTCGAC 1 cut(s) 148
SaqAI TTAA 3 cut(s) 656, 1235, 1287
Sau3AI GATC 6 cut(s) 352, 529, 639, 668, 1108, 1607
Sau96I GGNCC 2 cut(s) 1092, 1222
SchI GAGTC 3 cut(s) 134, 1003, 1015
ScrFI CCNGG 3 cut(s) 201, 350, 1473
SduI GDGCHC 3 cut(s) 522, 624, 1508
SfaNI GCATC 2 cut(s) 12, 323
SfcI CTRYAG 3 cut(s) 525, 846, 1189
SgrAI CRCCGGYG 1 cut(s) 626
SmiMI CAYNNNNRTG 1 cut(s) 491
SmlI CTYRAG 2 cut(s) 964, 1328
SmoI CTYRAG 2 cut(s) 964, 1328
Sse9I AATT 4 cut(s) 500, 556, 902, 1350
SsiI CCGC 5 cut(s) 196, 270, 1103, 1363, 1391
SspI AATATT 1 cut(s) 1645
SspMI CTAG 5 cut(s) 795, 927, 978, 1038, 1524
SstI GAGCTC 2 cut(s) 624, 1508
StyD4I CCNGG 3 cut(s) 199, 348, 1471
StyI CCWWGG 2 cut(s) 1424, 1564
TaaI ACNGT 3 cut(s) 943, 1033, 1498
TaiI ACGT 3 cut(s) 17, 1060, 1514
TaqII GACCGA 2 cut(s) 886, 1593
TasI AATT 4 cut(s) 500, 556, 902, 1350
TauI GCSGC 2 cut(s) 272, 1393
TfiI GAWTC 4 cut(s) 8, 79, 449, 673
Tru1I TTAA 3 cut(s) 656, 1235, 1287
Tru9I TTAA 3 cut(s) 656, 1235, 1287
TscAI CASTG 4 cut(s) 105, 493, 699, 1126
TseFI GTSAC 1 cut(s) 1206
TseI GCWGC 9 cut(s) 25, 41, 254, 266, 305, 308, 311, 845, 1253
Tsp45I GTSAC 1 cut(s) 1206
TspDTI ATGAA 3 cut(s) 71, 549, 1206
TspGWI ACGGA 1 cut(s) 1148
TspRI CASTG 4 cut(s) 105, 493, 699, 1126
VneI GTGCAC 1 cut(s) 518
XapI RAATTY 3 cut(s) 500, 556, 902
XbaI TCTAGA 1 cut(s) 926
XmiI GTMKAC 2 cut(s) 149, 426
XspI CTAG 5 cut(s) 795, 927, 978, 1038, 1524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.