Rorug02G0574100

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001269
Physical Location & Seq
Forward (+)
69949656 .. 69951054
1399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0574100.1

Sequence Viewer

Length: 699 bp
ATGCCTGTGAAGTTGGAGGAGCTCATACAGAAGATCAACCAAGGGGAAGGTGATAAAGTCACTTGTGTCATTGCTGATGAGAGTTGCGGATGGGCTCTGGAAGTGGCACGAAAAATGAAGATCAGGCAGGCCCGCTTTTGGCGAGCAGCCGGCTTGGAAAAACAGCCGGCAGCTTCTGGGCAGATCGAAGACCCAACTTGCCTAGAATGGCTTGATCAACAATCACCTTCCTCAGTCATCTATGTTGCTTTTGGAAGCTTCACAGTTTTTGATCAAACCCAATTCCAAGAATTGGCTTTGGCTCTTGAACTGTCCAATAGGCCATTCCTTTGGGTTGTGAGGCCAGACATATCTGAAAGTACTCCCTACCCTGAAGGATATGAAGAAAGAGTAGGCTCTAAAGGGTTGATGGTGGGATGGGCCCCACAACAGAAGGTTCTGGCCCATCCTTCAATTGCTTGCTTCCTAAGCCACTGTGGTTGGAACTCTATCTTGGAAGGTCTAAGAAATGGGGTTCCTTTCCTGTGTTGGCCATATTTTGCTGACCAGTTCCTTAATGAGAGCTACGTCTGTGATATTTGGAAGGTGGGATTAAGGCTTGATAAGAATAAAAGTGGGATCATAACGAAAGGAGAAATCAAGAACAAGTCCTTGGCGGCAAAAAATTCAGTGCCAGGGCTTCAAAACTCAAGGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

232

Amino Acids

26.0

Weight (kDa)

6.18

Isoelectric Point (pI)

48.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UDPGT PF00201 76 - 194 2.1e-20 UDP-glucoronosyl and UDP-glucosyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 292
AciI CCGC 3 cut(s) 87, 133, 656
AclWI GGATC 1 cut(s) 626
AcoI YGGCCR 1 cut(s) 530
AcsI RAATTY 1 cut(s) 664
AcuI CTGAAG 1 cut(s) 393
AfaI GTAC 1 cut(s) 361
AfiI CCNNNNNNNGG 2 cut(s) 138, 292
AgsI TTSAA 3 cut(s) 308, 453, 683
AjnI CCWGG 1 cut(s) 673
AjuI GAANNNNNNNTTGG 6 cut(s) 308, 340, 476, 508, 635, 667
AluBI AGCT 4 cut(s) 22, 173, 258, 564
AluI AGCT 4 cut(s) 22, 173, 258, 564
Alw21I GWGCWC 1 cut(s) 24
AlwI GGATC 1 cut(s) 626
AlwNI CAGNNNCTG 1 cut(s) 176
AoxI GGCC 6 cut(s) 129, 320, 341, 420, 441, 530
ApaI GGGCCC 1 cut(s) 424
ApeKI GCWGC 2 cut(s) 146, 170
ApoI RAATTY 1 cut(s) 664
AspS9I GGNCC 4 cut(s) 130, 420, 421, 442
AsuHPI GGTGA 2 cut(s) 62, 216
BaeGI GKGCMC 1 cut(s) 424
BalI TGGCCA 1 cut(s) 532
BanII GRGCYC 3 cut(s) 24, 97, 424
BbsI GAAGAC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 24
BbvI GCAGC 2 cut(s) 158, 182
BccI CCATC 4 cut(s) 84, 403, 411, 453
BciT130I CCWGG 1 cut(s) 675
BclI TGATCA 2 cut(s) 214, 271
BfaI CTAG 2 cut(s) 203, 697
BisI GCNGC 3 cut(s) 147, 171, 657
BlsI GCNGC 3 cut(s) 148, 172, 658
BmcAI AGTACT 1 cut(s) 361
Bme1390I CCNGG 1 cut(s) 675
BmgT120I GGNCC 4 cut(s) 130, 420, 421, 442
BmiI GGNNCC 3 cut(s) 422, 423, 516
BmrFI CCNGG 1 cut(s) 675
BpiI GAAGAC 1 cut(s) 195
Bpu10I CCTNAGC 1 cut(s) 467
BpuEI CTTGAG 1 cut(s) 673
BsaJI CCNNGG 3 cut(s) 40, 651, 674
Bsc4I CCNNNNNNNGG 2 cut(s) 138, 292
Bse118I RCCGGY 2 cut(s) 149, 166
Bse1I ACTGG 1 cut(s) 547
Bse3DI GCAATG 1 cut(s) 69
BseBI CCWGG 1 cut(s) 675
BseDI CCNNGG 3 cut(s) 40, 651, 674
BseGI GGATG 3 cut(s) 95, 422, 445
BseLI CCNNNNNNNGG 2 cut(s) 138, 292
BseMI GCAATG 1 cut(s) 69
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 1 cut(s) 547
BseRI GAGGAG 1 cut(s) 32
BseSI GKGCMC 1 cut(s) 424
BseXI GCAGC 2 cut(s) 158, 182
BshFI GGCC 6 cut(s) 131, 322, 343, 422, 443, 532
BsiHKAI GWGCWC 1 cut(s) 24
BsiSI CCGG 2 cut(s) 150, 167
BslI CCNNNNNNNGG 2 cut(s) 138, 292
BsnI GGCC 6 cut(s) 131, 322, 343, 422, 443, 532
Bsp120I GGGCCC 1 cut(s) 420
Bsp1286I GDGCHC 3 cut(s) 24, 97, 424
Bsp143I GATC 6 cut(s) 33, 120, 183, 214, 271, 618
BspACI CCGC 3 cut(s) 87, 133, 656
BspANI GGCC 6 cut(s) 131, 322, 343, 422, 443, 532
BspCNI CTCAG 1 cut(s) 245
BspLI GGNNCC 3 cut(s) 422, 423, 516
BspPI GGATC 1 cut(s) 626
BsrDI GCAATG 1 cut(s) 69
BsrFI RCCGGY 2 cut(s) 149, 166
BsrI ACTGG 1 cut(s) 547
BssAI RCCGGY 2 cut(s) 149, 166
BssECI CCNNGG 3 cut(s) 40, 651, 674
BssMI GATC 6 cut(s) 33, 120, 183, 214, 271, 618
BssT1I CCWWGG 2 cut(s) 40, 651
Bst2UI CCWGG 1 cut(s) 675
Bst4CI ACNGT 3 cut(s) 265, 312, 476
BstC8I GCNNGC 6 cut(s) 129, 133, 144, 151, 168, 460
BstDEI CTNAG 3 cut(s) 232, 467, 503
BstF5I GGATG 3 cut(s) 95, 422, 445
BstKTI GATC 6 cut(s) 36, 123, 186, 217, 274, 621
BstMBI GATC 6 cut(s) 33, 120, 183, 214, 271, 618
BstMWI GCNNNNNNNGC 1 cut(s) 468
BstNI CCWGG 1 cut(s) 675
BstSCI CCNGG 1 cut(s) 673
BstSLI GKGCMC 1 cut(s) 424
BstV1I GCAGC 2 cut(s) 158, 182
BstV2I GAAGAC 1 cut(s) 195
BstXI CCANNNNNNTGG 1 cut(s) 330
BsuRI GGCC 6 cut(s) 131, 322, 343, 422, 443, 532
BtsCI GGATG 3 cut(s) 95, 422, 445
BtsIMutI CAGTG 2 cut(s) 472, 675
Cac8I GCNNGC 6 cut(s) 129, 133, 144, 151, 168, 460
CaiI CAGNNNCTG 1 cut(s) 176
Cfr10I RCCGGY 2 cut(s) 149, 166
Cfr13I GGNCC 4 cut(s) 130, 420, 421, 442
Csp6I GTAC 1 cut(s) 360
CspCI CAANNNNNGTGG 2 cut(s) 461, 496
CviQI GTAC 1 cut(s) 360
DdeI CTNAG 3 cut(s) 232, 467, 503
DpnI GATC 6 cut(s) 35, 122, 185, 216, 273, 620
DpnII GATC 6 cut(s) 33, 120, 183, 214, 271, 618
EaeI YGGCCR 1 cut(s) 530
Ecl136II GAGCTC 1 cut(s) 22
Eco130I CCWWGG 2 cut(s) 40, 651
Eco24I GRGCYC 3 cut(s) 24, 97, 424
Eco53kI GAGCTC 1 cut(s) 22
Eco57I CTGAAG 1 cut(s) 393
EcoICRI GAGCTC 1 cut(s) 22
EcoO109I RGGNCCY 1 cut(s) 421
EcoRII CCWGG 1 cut(s) 673
EcoT14I CCWWGG 2 cut(s) 40, 651
EcoT38I GRGCYC 3 cut(s) 24, 97, 424
ErhI CCWWGG 2 cut(s) 40, 651
FaiI YATR 6 cut(s) 26, 243, 350, 381, 535, 623
FauI CCCGC 1 cut(s) 140
FbaI TGATCA 2 cut(s) 214, 271
Fnu4HI GCNGC 3 cut(s) 147, 171, 657
FokI GGATG 3 cut(s) 102, 429, 432
FriOI GRGCYC 3 cut(s) 24, 97, 424
Fsp4HI GCNGC 3 cut(s) 147, 171, 657
FspBI CTAG 2 cut(s) 203, 697
GluI GCNGC 3 cut(s) 147, 171, 657
HaeIII GGCC 6 cut(s) 131, 322, 343, 422, 443, 532
HapII CCGG 2 cut(s) 150, 167
HindIII AAGCTT 1 cut(s) 256
HpaII CCGG 2 cut(s) 150, 167
HphI GGTGA 2 cut(s) 62, 216
Hpy188I TCNGA 1 cut(s) 355
Hpy188III TCNNGA 3 cut(s) 98, 305, 640
HpyAV CCTTC 7 cut(s) 41, 237, 368, 427, 459, 491, 577
HpyCH4III ACNGT 3 cut(s) 265, 312, 476
HpyCH4IV ACGT 1 cut(s) 567
HpyF10VI GCNNNNNNNGC 1 cut(s) 468
HpyF3I CTNAG 3 cut(s) 232, 467, 503
HpySE526I ACGT 1 cut(s) 567
KroI GCCGGC 2 cut(s) 149, 166
KroNI GCCGGC 2 cut(s) 151, 168
Ksp22I TGATCA 2 cut(s) 214, 271
Kzo9I GATC 6 cut(s) 33, 120, 183, 214, 271, 618
LmnI GCTCC 1 cut(s) 19
Lsp1109I GCAGC 2 cut(s) 158, 182
MaeI CTAG 2 cut(s) 203, 697
MaeII ACGT 1 cut(s) 567
MaeIII GTNAC 1 cut(s) 58
MalI GATC 6 cut(s) 35, 122, 185, 216, 273, 620
MboI GATC 6 cut(s) 33, 120, 183, 214, 271, 618
MboII GAAGA 4 cut(s) 43, 130, 200, 395
MfeI CAATTG 1 cut(s) 453
MhlI GDGCHC 3 cut(s) 24, 97, 424
MlsI TGGCCA 1 cut(s) 532
MluCI AATT 4 cut(s) 281, 290, 453, 664
MluNI TGGCCA 1 cut(s) 532
MmeI TCCRAC 1 cut(s) 461
MnlI CCTC 3 cut(s) 10, 241, 333
Mox20I TGGCCA 1 cut(s) 532
MroNI GCCGGC 2 cut(s) 149, 166
MscI TGGCCA 1 cut(s) 532
MseI TTAA 2 cut(s) 555, 593
Msp20I TGGCCA 1 cut(s) 532
MspI CCGG 2 cut(s) 150, 167
MspR9I CCNGG 1 cut(s) 675
MunI CAATTG 1 cut(s) 453
MvaI CCWGG 1 cut(s) 675
MwoI GCNNNNNNNGC 1 cut(s) 468
NaeI GCCGGC 2 cut(s) 151, 168
NdeII GATC 6 cut(s) 33, 120, 183, 214, 271, 618
NgoMIV GCCGGC 2 cut(s) 149, 166
NlaIV GGNNCC 3 cut(s) 422, 423, 516
NmuCI GTSAC 1 cut(s) 58
PdiI GCCGGC 2 cut(s) 151, 168
PflMI CCANNNNNTGG 1 cut(s) 292
PkrI GCNGC 3 cut(s) 148, 172, 658
Psp124BI GAGCTC 1 cut(s) 24
Psp6I CCWGG 1 cut(s) 673
PspGI CCWGG 1 cut(s) 673
PspN4I GGNNCC 3 cut(s) 422, 423, 516
PspOMI GGGCCC 1 cut(s) 420
PspPI GGNCC 4 cut(s) 130, 420, 421, 442
PstNI CAGNNNCTG 1 cut(s) 176
RsaI GTAC 1 cut(s) 361
RsaNI GTAC 1 cut(s) 360
SacI GAGCTC 1 cut(s) 24
SaqAI TTAA 2 cut(s) 555, 593
SatI GCNGC 3 cut(s) 147, 171, 657
Sau3AI GATC 6 cut(s) 33, 120, 183, 214, 271, 618
Sau96I GGNCC 4 cut(s) 130, 420, 421, 442
ScaI AGTACT 1 cut(s) 361
ScrFI CCNGG 1 cut(s) 675
SduI GDGCHC 3 cut(s) 24, 97, 424
SmlI CTYRAG 1 cut(s) 688
SmoI CTYRAG 1 cut(s) 688
Sse9I AATT 4 cut(s) 281, 290, 453, 664
SsiI CCGC 3 cut(s) 87, 133, 656
SspMI CTAG 2 cut(s) 203, 697
SstI GAGCTC 1 cut(s) 24
StyD4I CCNGG 1 cut(s) 673
StyI CCWWGG 2 cut(s) 40, 651
TaaI ACNGT 3 cut(s) 265, 312, 476
TaiI ACGT 1 cut(s) 570
TaqI TCGA 1 cut(s) 186
TasI AATT 4 cut(s) 281, 290, 453, 664
TatI WGTACW 1 cut(s) 359
TauI GCSGC 1 cut(s) 659
Tru1I TTAA 2 cut(s) 555, 593
Tru9I TTAA 2 cut(s) 555, 593
TscAI CASTG 2 cut(s) 479, 675
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 2 cut(s) 146, 170
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 2 cut(s) 131, 396
TspRI CASTG 2 cut(s) 479, 675
Van91I CCANNNNNTGG 1 cut(s) 292
XapI RAATTY 1 cut(s) 664
XspI CTAG 2 cut(s) 203, 697
ZrmI AGTACT 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.