Rorug02G0574000

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001269
Physical Location & Seq
Forward (+)
69946522 .. 69948255
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0574000.1

Sequence Viewer

Length: 1365 bp
ATGAGCAAGCCACATATTATAGCTATTCCTTTCCCGGCACAAGGCCATGTAATGCCCTTGATGGAGTTCTCGCAGTGCTTAGCACGTCATGGCTTCAAAGTCACATTTGTGAACACAGAACATATTCACAAGCAAATTGTGAATGCAATATCCGATGAAAGTCATATAAGGAATGATCATGTTCATCTGGTTTCAATTCCAGATGGGTTAGAATCCCAAGAGGAAAGGAATGGGCCAGGGCTGCTATCCAAAGCAATACAAGCAATCATGCACCAGAATTTGGAGGATCTCATAGAGAAGATCAACAAAGAGGAAAGTGCAAAAATCACTTGTCTCATAGCTGATGAGAGTTGTGGGTGGGCTCTGGAAGTGGCACAAAAATTGAAGATTGCGAGGGTGGTTGCCTTTTGGCCTGCAGCAGCTGCAACTTTGGTATTGAACTTTTGTATCCCAAAATTAATTCATGAAGGAATCATTGACGATGATGGAACTGTATTGAAAAGCCAGATGGTTCAGTTGGCACCAAAAATGCCCATGATTAAATCTACAAACTTTGTGTGGGCGTGTGCAGGAAATTCAAGCACTCAGAAAATCCTATTCCAATTTATGGAAAGAACCAACAAGAATGCAAAATTGGTAGACTGGCTTGTTTGCAACTCAGCATACGAAATGGAGCCAGCAGCATTCGCTTTGGAACCACAGATATTACCAATAGGCCCGCTTTTGGCTAGCAGCCGCCTTGGCCACTCAGCAGGCAGCTTGTGGCCAACAGACTCAACTTGCCTAAATTGGTTGGATAAAAAACCACCTTGCTCAGTGATCTATGTTGCATTTGGCAGCACAGCAGTTTTCAATCAAACCCAATTCCAAGAACTGGCTTTAGCTCTTGAGTTGTCTAATAGGCCATTCCTCTGGGTTGTGAGACCAGATACCAGTGATAACATTCCCCACCCTGAAGGATATCAAGACAGAGTAGGATCTAATGGGCTAATGGTGAGCTGGGCCCCACAACAGAACGTTCTGGCCCATCCTTCAATTGCTTGCTTCCTAAGTCATTGTGGGTGGAACTCTACCATGGAAGGTGTCAGCAATGGGGTTCCTTTCTTGTGCTGGCCATACTTTGCTGACCAGTTCATCAATGAGAGCTACATTTGCGATGTTTGGGAGGTCGGATTGGGGTTTGATAAGAATGAAAGTGGGATCATTACTCAAGGAGAAATCAAGAACAAAATCGAGCACTTGCTTGGTGATAAAGACTTCAAAGCAAGGGCTTCCAAACTCAAGGAAATGGCCATGACAACTGTCAAAGAAGGAGGCCAATCTAACAAGATATTGAAGAATTTTATTGAATGGATTAAGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

454

Amino Acids

50.51

Weight (kDa)

5.93

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_N PF26168 6 - 146 2.8e-08 Glycosyltransferase, N-terminal domain
UDPGT PF00201 273 - 428 9.8e-23 UDP-glucoronosyl and UDP-glucosyl transferase
EryCIII-like_C PF06722 331 - 431 1.8e-06 Erythromycin biosynthesis protein CIII-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 520
AccB7I CCANNNNNTGG 3 cut(s) 280, 607, 874
AccI GTMKAC 1 cut(s) 639
AciI CCGC 2 cut(s) 719, 736
AclI AACGTT 1 cut(s) 1017
AclWI GGATC 3 cut(s) 294, 985, 1208
AcoI YGGCCR 4 cut(s) 742, 764, 1112, 1290
AcsI RAATTY 3 cut(s) 277, 574, 1339
AcuI CTGAAG 1 cut(s) 975
AfiI CCNNNNNNNGG 5 cut(s) 41, 280, 607, 724, 874
AjiI CACGTC 1 cut(s) 86
AjnI CCWGG 1 cut(s) 235
AjuI GAANNNNNNNTTGG 2 cut(s) 617, 649
AleI CACNNNNGTG 1 cut(s) 107
AluBI AGCT 7 cut(s) 23, 341, 422, 759, 884, 999, 1146
AluI AGCT 7 cut(s) 23, 341, 422, 759, 884, 999, 1146
Alw21I GWGCWC 1 cut(s) 1239
Alw26I GTCTC 2 cut(s) 338, 916
AlwI GGATC 3 cut(s) 294, 985, 1208
AlwNI CAGNNNCTG 1 cut(s) 422
ApaI GGGCCC 1 cut(s) 1006
ApeKI GCWGC 8 cut(s) 241, 416, 419, 422, 680, 732, 756, 837
ApoI RAATTY 3 cut(s) 277, 574, 1339
AseI ATTAAT 1 cut(s) 458
Asp700I GAANNNNTTC 1 cut(s) 123
AspS9I GGNCC 5 cut(s) 233, 716, 1002, 1003, 1024
AsuC2I CCSGG 1 cut(s) 35
AsuHPI GGTGA 2 cut(s) 1006, 1259
AsuNHI GCTAGC 1 cut(s) 728
BaeGI GKGCMC 1 cut(s) 1006
BaeI ACNNNNGTAYC 2 cut(s) 430, 463
BalI TGGCCA 4 cut(s) 744, 766, 1114, 1292
BanI GGYRCC 1 cut(s) 520
BanII GRGCYC 2 cut(s) 364, 1006
Bbv12I GWGCWC 1 cut(s) 1239
BbvI GCAGC 8 cut(s) 228, 409, 428, 431, 692, 744, 768, 849
BccI CCATC 5 cut(s) 55, 197, 479, 502, 1035
BciT130I CCWGG 1 cut(s) 237
BciVI GTATCC 1 cut(s) 458
BclI TGATCA 1 cut(s) 175
BcnI CCSGG 1 cut(s) 35
BcoDI GTCTC 2 cut(s) 338, 916
BfaI CTAG 1 cut(s) 729
BfmI CTRYAG 1 cut(s) 414
BfuI GTATCC 1 cut(s) 458
BglI GCCNNNNNGGC 1 cut(s) 741
BisI GCNGC 9 cut(s) 242, 417, 420, 423, 681, 733, 736, 757, 838
BlpI GCTNAGC 1 cut(s) 79
BlsI GCNGC 9 cut(s) 243, 418, 421, 424, 682, 734, 737, 758, 839
Bme1390I CCNGG 2 cut(s) 35, 237
BmgBI CACGTC 1 cut(s) 86
BmgT120I GGNCC 5 cut(s) 233, 716, 1002, 1003, 1024
BmiI GGNNCC 6 cut(s) 522, 675, 696, 1004, 1005, 1098
BmrFI CCNGG 2 cut(s) 35, 237
BmtI GCTAGC 1 cut(s) 732
BoxI GACNNNNGTC 1 cut(s) 1301
Bpu1102I GCTNAGC 1 cut(s) 79
BpuEI CTTGAG 3 cut(s) 908, 1194, 1265
BpuMI CCSGG 1 cut(s) 35
BsaI GGTCTC 1 cut(s) 916
BsaJI CCNNGG 3 cut(s) 236, 739, 1074
Bsc4I CCNNNNNNNGG 5 cut(s) 41, 280, 607, 724, 874
Bse1I ACTGG 4 cut(s) 647, 879, 933, 1129
Bse3DI GCAATG 1 cut(s) 1096
BseBI CCWGG 1 cut(s) 237
BseDI CCNNGG 3 cut(s) 236, 739, 1074
BseGI GGATG 1 cut(s) 1027
BseLI CCNNNNNNNGG 5 cut(s) 41, 280, 607, 724, 874
BseMI GCAATG 1 cut(s) 1096
BseMII CTCAG 4 cut(s) 599, 672, 762, 828
BseNI ACTGG 4 cut(s) 647, 879, 933, 1129
BseSI GKGCMC 1 cut(s) 1006
BseXI GCAGC 8 cut(s) 228, 409, 428, 431, 692, 744, 768, 849
BseYI CCCAGC 1 cut(s) 999
BsgI GTGCAG 1 cut(s) 588
BshNI GGYRCC 1 cut(s) 520
BsiHKAI GWGCWC 1 cut(s) 1239
BsiSI CCGG 1 cut(s) 35
BslI CCNNNNNNNGG 5 cut(s) 41, 280, 607, 724, 874
BsmAI GTCTC 2 cut(s) 338, 916
BsmI GAATGC 3 cut(s) 148, 631, 683
Bso31I GGTCTC 1 cut(s) 916
Bsp120I GGGCCC 1 cut(s) 1002
Bsp1286I GDGCHC 3 cut(s) 364, 1006, 1239
Bsp143I GATC 6 cut(s) 175, 286, 300, 819, 977, 1200
Bsp1720I GCTNAGC 1 cut(s) 79
Bsp19I CCATGG 1 cut(s) 1074
BspACI CCGC 2 cut(s) 719, 736
BspCNI CTCAG 4 cut(s) 598, 671, 761, 827
BspHI TCATGA 1 cut(s) 463
BspLI GGNNCC 6 cut(s) 522, 675, 696, 1004, 1005, 1098
BspMAI CTGCAG 1 cut(s) 418
BspOI GCTAGC 1 cut(s) 732
BspPI GGATC 3 cut(s) 294, 985, 1208
BspT107I GGYRCC 1 cut(s) 520
BspTNI GGTCTC 1 cut(s) 916
BsrDI GCAATG 1 cut(s) 1096
BsrI ACTGG 4 cut(s) 647, 879, 933, 1129
BssECI CCNNGG 3 cut(s) 236, 739, 1074
BssMI GATC 6 cut(s) 175, 286, 300, 819, 977, 1200
BssT1I CCWWGG 2 cut(s) 739, 1074
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 2 cut(s) 493, 1303
BstAPI GCANNNNNTGC 1 cut(s) 422
BstC8I GCNNGC 8 cut(s) 8, 414, 678, 719, 730, 754, 1042, 1112
BstDEI CTNAG 6 cut(s) 79, 585, 658, 748, 814, 1049
BstDSI CCRYGG 1 cut(s) 1074
BstF5I GGATG 1 cut(s) 1027
BstKTI GATC 6 cut(s) 178, 289, 303, 822, 980, 1203
BstMAI GTCTC 2 cut(s) 338, 916
BstMBI GATC 6 cut(s) 175, 286, 300, 819, 977, 1200
BstMWI GCNNNNNNNGC 6 cut(s) 241, 260, 422, 686, 741, 1152
BstNI CCWGG 1 cut(s) 237
BstPAI GACNNNNGTC 1 cut(s) 1301
BstSCI CCNGG 2 cut(s) 33, 235
BstSFI CTRYAG 1 cut(s) 414
BstSLI GKGCMC 1 cut(s) 1006
BstV1I GCAGC 8 cut(s) 228, 409, 428, 431, 692, 744, 768, 849
BstX2I RGATCY 2 cut(s) 286, 977
BstXI CCANNNNNNTGG 1 cut(s) 912
BstYI RGATCY 2 cut(s) 286, 977
BsuI GTATCC 1 cut(s) 458
BtgI CCRYGG 1 cut(s) 1074
BtgZI GCGATG 1 cut(s) 1170
BtrI CACGTC 1 cut(s) 86
BtsCI GGATG 1 cut(s) 1027
BtsI GCAGTG 1 cut(s) 80
BtsIMutI CAGTG 3 cut(s) 80, 822, 940
Cac8I GCNNGC 8 cut(s) 8, 414, 678, 719, 730, 754, 1042, 1112
CaiI CAGNNNCTG 1 cut(s) 422
CciI TCATGA 1 cut(s) 463
Cfr13I GGNCC 5 cut(s) 233, 716, 1002, 1003, 1024
CviAII CATG 8 cut(s) 47, 89, 179, 268, 464, 535, 1075, 1294
DdeI CTNAG 6 cut(s) 79, 585, 658, 748, 814, 1049
DpnI GATC 6 cut(s) 177, 288, 302, 821, 979, 1202
DpnII GATC 6 cut(s) 175, 286, 300, 819, 977, 1200
EaeI YGGCCR 4 cut(s) 742, 764, 1112, 1290
Eco130I CCWWGG 2 cut(s) 739, 1074
Eco24I GRGCYC 2 cut(s) 364, 1006
Eco31I GGTCTC 1 cut(s) 916
Eco32I GATATC 1 cut(s) 962
Eco57I CTGAAG 1 cut(s) 975
EcoO109I RGGNCCY 1 cut(s) 1003
EcoRII CCWGG 1 cut(s) 235
EcoRV GATATC 1 cut(s) 962
EcoT14I CCWWGG 2 cut(s) 739, 1074
EcoT38I GRGCYC 2 cut(s) 364, 1006
ErhI CCWWGG 2 cut(s) 739, 1074
FaeI CATG 8 cut(s) 50, 92, 182, 271, 467, 538, 1078, 1297
FatI CATG 8 cut(s) 46, 88, 178, 267, 463, 534, 1074, 1293
FauI CCCGC 1 cut(s) 726
FbaI TGATCA 1 cut(s) 175
FblI GTMKAC 1 cut(s) 639
Fnu4HI GCNGC 9 cut(s) 242, 417, 420, 423, 681, 733, 736, 757, 838
FokI GGATG 1 cut(s) 1014
FriOI GRGCYC 2 cut(s) 364, 1006
Fsp4HI GCNGC 9 cut(s) 242, 417, 420, 423, 681, 733, 736, 757, 838
FspBI CTAG 1 cut(s) 729
GluI GCNGC 9 cut(s) 242, 417, 420, 423, 681, 733, 736, 757, 838
GsaI CCCAGC 1 cut(s) 1003
HapII CCGG 1 cut(s) 35
Hin1II CATG 8 cut(s) 50, 92, 182, 271, 467, 538, 1078, 1297
HinfI GANTC 3 cut(s) 212, 471, 773
HpaII CCGG 1 cut(s) 35
HphI GGTGA 2 cut(s) 1006, 1259
Hpy166II GTNNAC 2 cut(s) 112, 640
Hpy188I TCNGA 3 cut(s) 154, 588, 1172
Hpy188III TCNNGA 6 cut(s) 200, 365, 464, 887, 965, 1222
Hpy8I GTNNAC 2 cut(s) 112, 640
HpyAV CCTTC 5 cut(s) 461, 950, 1041, 1073, 1304
HpyCH4III ACNGT 2 cut(s) 493, 1303
HpyCH4IV ACGT 2 cut(s) 85, 1017
HpyCH4V TGCA 9 cut(s) 146, 271, 320, 416, 425, 569, 629, 654, 830
HpyF10VI GCNNNNNNNGC 6 cut(s) 241, 260, 422, 686, 741, 1152
HpyF3I CTNAG 6 cut(s) 79, 585, 658, 748, 814, 1049
HpySE526I ACGT 2 cut(s) 85, 1017
Hsp92II CATG 8 cut(s) 50, 92, 182, 271, 467, 538, 1078, 1297
Ksp22I TGATCA 1 cut(s) 175
Kzo9I GATC 6 cut(s) 175, 286, 300, 819, 977, 1200
LmnI GCTCC 1 cut(s) 673
Lsp1109I GCAGC 8 cut(s) 228, 409, 428, 431, 692, 744, 768, 849
MaeI CTAG 1 cut(s) 729
MaeII ACGT 2 cut(s) 85, 1017
MaeIII GTNAC 1 cut(s) 100
MalI GATC 6 cut(s) 177, 288, 302, 821, 979, 1202
MboI GATC 6 cut(s) 175, 286, 300, 819, 977, 1200
MboII GAAGA 3 cut(s) 310, 397, 1348
MfeI CAATTG 1 cut(s) 1035
MflI RGATCY 2 cut(s) 286, 977
MhlI GDGCHC 3 cut(s) 364, 1006, 1239
MlsI TGGCCA 4 cut(s) 744, 766, 1114, 1292
MluNI TGGCCA 4 cut(s) 744, 766, 1114, 1292
MlyI GAGTC 1 cut(s) 767
MmeI TCCRAC 2 cut(s) 774, 1150
MnlI CCTC 7 cut(s) 214, 277, 304, 387, 920, 1159, 1307
Mox20I TGGCCA 4 cut(s) 744, 766, 1114, 1292
MroXI GAANNNNTTC 1 cut(s) 123
MscI TGGCCA 4 cut(s) 744, 766, 1114, 1292
MseI TTAA 3 cut(s) 458, 540, 1356
MslI CAYNNNNRTG 1 cut(s) 107
Msp20I TGGCCA 4 cut(s) 744, 766, 1114, 1292
MspA1I CMGCKG 1 cut(s) 422
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 2 cut(s) 35, 237
MunI CAATTG 1 cut(s) 1035
Mva1269I GAATGC 3 cut(s) 148, 631, 683
MvaI CCWGG 1 cut(s) 237
MwoI GCNNNNNNNGC 6 cut(s) 241, 260, 422, 686, 741, 1152
NciI CCSGG 1 cut(s) 35
NcoI CCATGG 1 cut(s) 1074
NdeII GATC 6 cut(s) 175, 286, 300, 819, 977, 1200
NheI GCTAGC 1 cut(s) 728
NlaIII CATG 8 cut(s) 50, 92, 182, 271, 467, 538, 1078, 1297
NlaIV GGNNCC 6 cut(s) 522, 675, 696, 1004, 1005, 1098
NmuCI GTSAC 1 cut(s) 100
OliI CACNNNNGTG 1 cut(s) 107
PagI TCATGA 1 cut(s) 463
PctI GAATGC 3 cut(s) 148, 631, 683
PdmI GAANNNNTTC 1 cut(s) 123
PfeI GAWTC 2 cut(s) 212, 471
PflMI CCANNNNNTGG 3 cut(s) 280, 607, 874
PkrI GCNGC 9 cut(s) 243, 418, 421, 424, 682, 734, 737, 758, 839
PleI GAGTC 1 cut(s) 767
PpsI GAGTC 1 cut(s) 767
PshAI GACNNNNGTC 1 cut(s) 1301
PshBI ATTAAT 1 cut(s) 458
Psp1406I AACGTT 1 cut(s) 1017
Psp6I CCWGG 1 cut(s) 235
PspFI CCCAGC 1 cut(s) 999
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 6 cut(s) 522, 675, 696, 1004, 1005, 1098
PspOMI GGGCCC 1 cut(s) 1002
PspPI GGNCC 5 cut(s) 233, 716, 1002, 1003, 1024
PstI CTGCAG 1 cut(s) 418
PstNI CAGNNNCTG 1 cut(s) 422
PsuI RGATCY 2 cut(s) 286, 977
PvuII CAGCTG 1 cut(s) 422
RseI CAYNNNNRTG 1 cut(s) 107
SaqAI TTAA 3 cut(s) 458, 540, 1356
SatI GCNGC 9 cut(s) 242, 417, 420, 423, 681, 733, 736, 757, 838
Sau3AI GATC 6 cut(s) 175, 286, 300, 819, 977, 1200
Sau96I GGNCC 5 cut(s) 233, 716, 1002, 1003, 1024
SchI GAGTC 1 cut(s) 767
ScrFI CCNGG 2 cut(s) 35, 237
SduI GDGCHC 3 cut(s) 364, 1006, 1239
SfcI CTRYAG 1 cut(s) 414
SmiMI CAYNNNNRTG 1 cut(s) 107
SmlI CTYRAG 3 cut(s) 887, 1209, 1280
SmoI CTYRAG 3 cut(s) 887, 1209, 1280
SsiI CCGC 2 cut(s) 719, 736
SspMI CTAG 1 cut(s) 729
StyD4I CCNGG 2 cut(s) 33, 235
StyI CCWWGG 2 cut(s) 739, 1074
TaaI ACNGT 2 cut(s) 493, 1303
TaiI ACGT 2 cut(s) 88, 1020
TaqI TCGA 1 cut(s) 1233
TauI GCSGC 1 cut(s) 738
TfiI GAWTC 2 cut(s) 212, 471
Tru1I TTAA 3 cut(s) 458, 540, 1356
Tru9I TTAA 3 cut(s) 458, 540, 1356
TscAI CASTG 3 cut(s) 80, 822, 940
TseFI GTSAC 1 cut(s) 100
TseI GCWGC 8 cut(s) 241, 416, 419, 422, 680, 732, 756, 837
Tsp45I GTSAC 1 cut(s) 100
TspDTI ATGAA 6 cut(s) 171, 173, 452, 480, 1123, 1206
TspRI CASTG 3 cut(s) 80, 822, 940
Van91I CCANNNNNTGG 3 cut(s) 280, 607, 874
VspI ATTAAT 1 cut(s) 458
XapI RAATTY 3 cut(s) 277, 574, 1339
XmiI GTMKAC 1 cut(s) 639
XmnI GAANNNNTTC 1 cut(s) 123
XspI CTAG 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.