pycom16g09260

TPX2 (targeting protein for Xklp2) protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001269
Physical Location & Seq
Reverse (-)
6196628 .. 6199101
2474 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g09260.4

Sequence Viewer

Length: 1446 bp
ATGTCAGAACCTCTCTCTTGGGAGAAATGGTCTGCGTTTTCCCACAATCGCTACTTAGAAGAAGTCGAGAAGTTCTCTAAGCCCGGCTCTGTTGCTCAGAAGAAAGCTTATTTTGAAGCTCACTACAAGAAAAAAGCTGCTGAGAAAGCAGCAGCCTTGCTTGAGCTGACAAATGCTGGTGCCTGTAATGTTACAGCGTCATCAGTAAATATGTATGCAAATTACGAGAGCCCCTCCATGGACATAAACTCGGCGAATGGTGGGAGCCAGATGGTCGTTGATAAAGAACAGGAAAATGTTGTTCCAAATTCTGAGGTAGAAGTATACTCAGCTGATGTGAATGGCCATAATCAAAGTGTTGAAGGGAATCAACTGGATACCGCCAAGGTGGCCAGAGCAGAGGCGGTAATTCAAGAAAGTGTTAATTTGGAAATATCTATTCACGCTGAAATCTCGAACCGGTTTGAAAATGGCAAAGACCAAAATGAGATTGTTGCCACCCTAGAAGAGAAGATGCCAAATAAGATCCTCCAGGAAGCTGCCGGTGAGGAGAATCTCGCTTCAGCGAGCAAGAAAAGATTGATGAACTCTTCCCCAAGGTTATCCACTAAAGGTAGACCACCCAAGGTACCATCGCCTCCTGCCAAACAAGCAACTCGTGTGCAAACCTTAAACGGGAAAAATGCCACTCAAATGGGTAAGAAGGTTTCATCGGACTTAGTTGACAAGAGGAGTTTAACTGCAAAATCTCTCCACATGTCAAAGCATTTCACTTCTCATGGTGGTGAAAGTGAAATCAGTAAAAGAACTTCGCCAATTGTTGAGAAGACTAGAGATTCGAGAAGTAGTACAACTACACTTAATATCTCCATGAATAATCCAGCTTCTCGACGAACTGCTATTAGGGCATCTGTTAGCGGGGTATTGATGCAGTCTTCAGTTGACCCATGGTCCAAAGATAGAAGGGCGAAAACATTACTTAATAAATCGGTAGCTGGACCAATTGCAGATGGGAAGTGGATATCCTCCTCAAAAGACGAATCTTCTACTGCAAATGGAAGTAAACCACGACCCCCTATTGTATCCTGTCCTTTTAGATTGAAGAGTGAAGATAGAGCAGAAAAGCGTAGAGAGTTCTACAAGAGTCTGGAAGAGAAGAAAAATGCCAAGGAGGCTGAACGTGACTTGGTGCAACTGAAATCCAATAAAAAGAAAAAAGCAGTGGGGGATGTTAATAAACTGCACAACAGCACTGCCTTAAAAGCGAAACTAAACCAAGATCTGTCATCGGCATCGCAATTTCCAAGTGATCACTTGAACAAGTCCTCTGCTACAGTCTCGGTCACCAAAACTTGGGAGGCACTCTACTGTCAGCAAGGTTCTGGACTCAAGCTCTCGACCATCGGTCAATTCTCACGGCTCCAAGTACGTCACACAAAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

482

Amino Acids

52.82

Weight (kDa)

9.62

Isoelectric Point (pI)

49.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 628
AccB1I GGYRCC 2 cut(s) 179, 628
AccB7I CCANNNNNTGG 1 cut(s) 1353
AccI GTMKAC 2 cut(s) 324, 616
AciI CCGC 3 cut(s) 381, 404, 918
AclWI GGATC 1 cut(s) 520
AcoI YGGCCR 2 cut(s) 343, 390
AcsI RAATTY 1 cut(s) 307
AcuI CTGAAG 2 cut(s) 546, 921
AfaI GTAC 3 cut(s) 630, 850, 1428
AfiI CCNNNNNNNGG 3 cut(s) 238, 675, 1353
AflIII ACRYGT 1 cut(s) 756
AgeI ACCGGT 1 cut(s) 459
AgsI TTSAA 6 cut(s) 116, 362, 413, 467, 1102, 1318
AhdI GACNNNNNGTC 2 cut(s) 949, 1404
AjnI CCWGG 1 cut(s) 531
AluBI AGCT 9 cut(s) 107, 119, 137, 166, 332, 539, 884, 995, 1393
AluI AGCT 9 cut(s) 107, 119, 137, 166, 332, 539, 884, 995, 1393
Alw26I GTCTC 1 cut(s) 1342
AlwI GGATC 1 cut(s) 520
AoxI GGCC 2 cut(s) 343, 390
ApeKI GCWGC 4 cut(s) 137, 149, 152, 539
ApoI RAATTY 1 cut(s) 307
ArsI GACNNNNNNTTYG 2 cut(s) 820, 852
AsiGI ACCGGT 1 cut(s) 459
Asp718I GGTACC 1 cut(s) 628
AspS9I GGNCC 2 cut(s) 951, 998
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 3 cut(s) 557, 797, 1336
AvaII GGWCC 2 cut(s) 951, 998
BalI TGGCCA 2 cut(s) 345, 392
BanI GGYRCC 2 cut(s) 179, 628
BanII GRGCYC 1 cut(s) 233
BarI GAAGNNNNNNTAC 2 cut(s) 793, 825
BauI CACGAG 1 cut(s) 657
BbsI GAAGAC 2 cut(s) 833, 927
BbvI GCAGC 4 cut(s) 124, 161, 164, 526
BccI CCATC 4 cut(s) 265, 640, 1004, 1409
BceAI ACGGC 1 cut(s) 1433
BciT130I CCWGG 1 cut(s) 533
BciVI GTATCC 2 cut(s) 370, 1093
BclI TGATCA 1 cut(s) 1309
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 1 cut(s) 1342
BfaI CTAG 2 cut(s) 503, 831
BfmI CTRYAG 1 cut(s) 1332
BfuI GTATCC 2 cut(s) 370, 1093
BglI GCCNNNNNGGC 2 cut(s) 389, 1172
BglII AGATCT 1 cut(s) 1279
BisI GCNGC 4 cut(s) 138, 150, 153, 540
BlsI GCNGC 4 cut(s) 139, 151, 154, 541
Bme1390I CCNGG 2 cut(s) 84, 533
Bme18I GGWCC 2 cut(s) 951, 998
BmeRI GACNNNNNGTC 2 cut(s) 949, 1404
BmgT120I GGNCC 2 cut(s) 951, 998
BmiI GGNNCC 4 cut(s) 181, 266, 630, 1421
BmrFI CCNGG 2 cut(s) 84, 533
BmsI GCATC 4 cut(s) 504, 917, 918, 1301
BpiI GAAGAC 2 cut(s) 833, 927
BplI GAGNNNNNCTC 4 cut(s) 59, 91, 218, 250
BpmI CTGGAG 1 cut(s) 515
BpuEI CTTGAG 2 cut(s) 182, 1373
BpuMI CCSGG 1 cut(s) 84
BsaJI CCNNGG 6 cut(s) 237, 384, 596, 624, 947, 1167
BsaWI WCCGGW 1 cut(s) 459
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bsc4I CCNNNNNNNGG 3 cut(s) 238, 675, 1353
Bse118I RCCGGY 2 cut(s) 459, 542
Bse1I ACTGG 1 cut(s) 378
BseBI CCWGG 1 cut(s) 533
BseDI CCNNGG 6 cut(s) 237, 384, 596, 624, 947, 1167
BseGI GGATG 1 cut(s) 1234
BseLI CCNNNNNNNGG 3 cut(s) 238, 675, 1353
BseMII CTCAG 4 cut(s) 110, 132, 303, 342
BseNI ACTGG 1 cut(s) 378
BseRI GAGGAG 3 cut(s) 563, 745, 1018
BseXI GCAGC 4 cut(s) 124, 161, 164, 526
BsgI GTGCAG 1 cut(s) 1226
BshFI GGCC 2 cut(s) 345, 392
BshNI GGYRCC 2 cut(s) 179, 628
BshTI ACCGGT 1 cut(s) 459
BsiSI CCGG 3 cut(s) 84, 460, 543
BslI CCNNNNNNNGG 3 cut(s) 238, 675, 1353
BsmAI GTCTC 1 cut(s) 1342
BsnI GGCC 2 cut(s) 345, 392
Bsp1286I GDGCHC 1 cut(s) 233
Bsp143I GATC 3 cut(s) 525, 1279, 1309
Bsp19I CCATGG 2 cut(s) 237, 947
BspACI CCGC 3 cut(s) 381, 404, 918
BspANI GGCC 2 cut(s) 345, 392
BspCNI CTCAG 4 cut(s) 109, 133, 304, 341
BspLI GGNNCC 4 cut(s) 181, 266, 630, 1421
BspPI GGATC 1 cut(s) 520
BspT107I GGYRCC 2 cut(s) 179, 628
BsrFI RCCGGY 2 cut(s) 459, 542
BsrI ACTGG 1 cut(s) 378
BssAI RCCGGY 2 cut(s) 459, 542
BssECI CCNNGG 6 cut(s) 237, 384, 596, 624, 947, 1167
BssMI GATC 3 cut(s) 525, 1279, 1309
BssNAI GTATAC 1 cut(s) 325
BssSI CACGAG 1 cut(s) 657
BssT1I CCWWGG 6 cut(s) 237, 384, 596, 624, 947, 1167
Bst1107I GTATAC 1 cut(s) 325
Bst2BI CACGAG 1 cut(s) 657
Bst2UI CCWGG 1 cut(s) 533
Bst4CI ACNGT 2 cut(s) 1336, 1370
Bst6I CTCTTC 4 cut(s) 501, 595, 1097, 1146
BstC8I GCNNGC 1 cut(s) 568
BstDEI CTNAG 7 cut(s) 55, 78, 96, 141, 312, 328, 718
BstDSI CCRYGG 2 cut(s) 237, 947
BstEII GGTNACC 1 cut(s) 1342
BstF5I GGATG 1 cut(s) 1234
BstKTI GATC 3 cut(s) 528, 1282, 1312
BstMAI GTCTC 1 cut(s) 1342
BstMBI GATC 3 cut(s) 525, 1279, 1309
BstMWI GCNNNNNNNGC 6 cut(s) 146, 389, 650, 905, 1172, 1262
BstNI CCWGG 1 cut(s) 533
BstNSI RCATGY 1 cut(s) 760
BstPI GGTNACC 1 cut(s) 1342
BstSCI CCNGG 2 cut(s) 82, 531
BstSFI CTRYAG 1 cut(s) 1332
BstV1I GCAGC 4 cut(s) 124, 161, 164, 526
BstV2I GAAGAC 2 cut(s) 833, 927
BstX2I RGATCY 2 cut(s) 525, 1279
BstXI CCANNNNNNTGG 1 cut(s) 694
BstYI RGATCY 2 cut(s) 525, 1279
BstZ17I GTATAC 1 cut(s) 325
BsuI GTATCC 2 cut(s) 370, 1093
BsuRI GGCC 2 cut(s) 345, 392
BtgI CCRYGG 2 cut(s) 237, 947
BtgZI GCGATG 2 cut(s) 618, 1278
BtsCI GGATG 1 cut(s) 1234
BtsI GCAGTG 2 cut(s) 1227, 1251
BtsIMutI CAGTG 2 cut(s) 1227, 1251
Cac8I GCNNGC 1 cut(s) 568
Cfr10I RCCGGY 2 cut(s) 459, 542
Cfr13I GGNCC 2 cut(s) 951, 998
CseI GACGC 1 cut(s) 186
Csp6I GTAC 3 cut(s) 629, 849, 1427
CspAI ACCGGT 1 cut(s) 459
CviAII CATG 5 cut(s) 238, 757, 779, 871, 948
CviQI GTAC 3 cut(s) 629, 849, 1427
DdeI CTNAG 7 cut(s) 55, 78, 96, 141, 312, 328, 718
DpnI GATC 3 cut(s) 527, 1281, 1311
DpnII GATC 3 cut(s) 525, 1279, 1309
DriI GACNNNNNGTC 2 cut(s) 949, 1404
EaeI YGGCCR 2 cut(s) 343, 390
Eam1104I CTCTTC 4 cut(s) 501, 595, 1097, 1146
Eam1105I GACNNNNNGTC 2 cut(s) 949, 1404
EarI CTCTTC 4 cut(s) 501, 595, 1097, 1146
Eco130I CCWWGG 6 cut(s) 237, 384, 596, 624, 947, 1167
Eco24I GRGCYC 1 cut(s) 233
Eco32I GATATC 1 cut(s) 1023
Eco47I GGWCC 2 cut(s) 951, 998
Eco57I CTGAAG 2 cut(s) 546, 921
Eco91I GGTNACC 1 cut(s) 1342
EcoO65I GGTNACC 1 cut(s) 1342
EcoRII CCWGG 1 cut(s) 531
EcoRV GATATC 1 cut(s) 1023
EcoT14I CCWWGG 6 cut(s) 237, 384, 596, 624, 947, 1167
EcoT38I GRGCYC 1 cut(s) 233
ErhI CCWWGG 6 cut(s) 237, 384, 596, 624, 947, 1167
FaeI CATG 5 cut(s) 241, 760, 782, 874, 951
FatI CATG 5 cut(s) 237, 756, 778, 870, 947
FauI CCCGC 1 cut(s) 911
FbaI TGATCA 1 cut(s) 1309
FblI GTMKAC 2 cut(s) 324, 616
Fnu4HI GCNGC 4 cut(s) 138, 150, 153, 540
FokI GGATG 1 cut(s) 1241
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 4 cut(s) 138, 150, 153, 540
FspBI CTAG 2 cut(s) 503, 831
GluI GCNGC 4 cut(s) 138, 150, 153, 540
GsuI CTGGAG 1 cut(s) 515
HaeIII GGCC 2 cut(s) 345, 392
HapII CCGG 3 cut(s) 84, 460, 543
HgaI GACGC 1 cut(s) 186
Hin1II CATG 5 cut(s) 241, 760, 782, 874, 951
HincII GTYRAC 2 cut(s) 724, 943
HindII GTYRAC 2 cut(s) 724, 943
HindIII AAGCTT 1 cut(s) 105
HinfI GANTC 6 cut(s) 367, 553, 836, 1040, 1144, 1386
HpaII CCGG 3 cut(s) 84, 460, 543
HphI GGTGA 3 cut(s) 557, 797, 1336
Hpy166II GTNNAC 5 cut(s) 325, 617, 724, 943, 1064
Hpy188I TCNGA 4 cut(s) 7, 99, 313, 715
Hpy188III TCNNGA 8 cut(s) 67, 413, 454, 840, 888, 1148, 1383, 1396
Hpy8I GTNNAC 5 cut(s) 325, 617, 724, 943, 1064
Hpy99I CGWCG 1 cut(s) 894
HpyAV CCTTC 3 cut(s) 356, 697, 957
HpyCH4III ACNGT 2 cut(s) 1336, 1370
HpyCH4IV ACGT 2 cut(s) 1180, 1429
HpyCH4V TGCA 8 cut(s) 218, 664, 743, 931, 1007, 1052, 1192, 1243
HpyF10VI GCNNNNNNNGC 6 cut(s) 146, 389, 650, 905, 1172, 1262
HpyF3I CTNAG 7 cut(s) 55, 78, 96, 141, 312, 328, 718
HpySE526I ACGT 2 cut(s) 1180, 1429
Hsp92II CATG 5 cut(s) 241, 760, 782, 874, 951
KpnI GGTACC 1 cut(s) 632
Ksp22I TGATCA 1 cut(s) 1309
Kzo9I GATC 3 cut(s) 525, 1279, 1309
LmnI GCTCC 2 cut(s) 264, 1425
Lsp1109I GCAGC 4 cut(s) 124, 161, 164, 526
LweI GCATC 4 cut(s) 504, 917, 918, 1301
MaeI CTAG 2 cut(s) 503, 831
MaeII ACGT 2 cut(s) 1180, 1429
MaeIII GTNAC 4 cut(s) 190, 1181, 1342, 1430
MalI GATC 3 cut(s) 527, 1281, 1311
MboI GATC 3 cut(s) 525, 1279, 1309
MfeI CAATTG 2 cut(s) 816, 1002
MflI RGATCY 2 cut(s) 525, 1279
MhlI GDGCHC 1 cut(s) 233
MlsI TGGCCA 2 cut(s) 345, 392
MluCI AATT 8 cut(s) 220, 307, 408, 424, 816, 1002, 1298, 1409
MluNI TGGCCA 2 cut(s) 345, 392
MlyI GAGTC 2 cut(s) 1153, 1380
Mox20I TGGCCA 2 cut(s) 345, 392
MscI TGGCCA 2 cut(s) 345, 392
MseI TTAA 7 cut(s) 423, 671, 737, 861, 981, 1233, 1259
MslI CAYNNNNRTG 2 cut(s) 692, 783
Msp20I TGGCCA 2 cut(s) 345, 392
MspA1I CMGCKG 1 cut(s) 332
MspI CCGG 3 cut(s) 84, 460, 543
MspR9I CCNGG 2 cut(s) 84, 533
MunI CAATTG 2 cut(s) 816, 1002
MvaI CCWGG 1 cut(s) 533
MwoI GCNNNNNNNGC 6 cut(s) 146, 389, 650, 905, 1172, 1262
NciI CCSGG 1 cut(s) 84
NcoI CCATGG 2 cut(s) 237, 947
NdeII GATC 3 cut(s) 525, 1279, 1309
NlaIII CATG 5 cut(s) 241, 760, 782, 874, 951
NlaIV GGNNCC 4 cut(s) 181, 266, 630, 1421
NmeAIII GCCGAG 1 cut(s) 230
NmuCI GTSAC 3 cut(s) 1181, 1342, 1430
NspI RCATGY 1 cut(s) 760
PciI ACATGT 1 cut(s) 756
PfeI GAWTC 4 cut(s) 367, 553, 836, 1040
PflMI CCANNNNNTGG 1 cut(s) 1353
PfoI TCCNGGA 1 cut(s) 531
PinAI ACCGGT 1 cut(s) 459
PkrI GCNGC 4 cut(s) 139, 151, 154, 541
PleI GAGTC 2 cut(s) 1152, 1380
PpsI GAGTC 2 cut(s) 1152, 1380
PscI ACATGT 1 cut(s) 756
Psp6I CCWGG 1 cut(s) 531
PspEI GGTNACC 1 cut(s) 1342
PspGI CCWGG 1 cut(s) 531
PspN4I GGNNCC 4 cut(s) 181, 266, 630, 1421
PspPI GGNCC 2 cut(s) 951, 998
PsuI RGATCY 2 cut(s) 525, 1279
PvuII CAGCTG 1 cut(s) 332
RsaI GTAC 3 cut(s) 630, 850, 1428
RsaNI GTAC 3 cut(s) 629, 849, 1427
RseI CAYNNNNRTG 2 cut(s) 692, 783
SaqAI TTAA 7 cut(s) 423, 671, 737, 861, 981, 1233, 1259
SatI GCNGC 4 cut(s) 138, 150, 153, 540
Sau3AI GATC 3 cut(s) 525, 1279, 1309
Sau96I GGNCC 2 cut(s) 951, 998
SchI GAGTC 2 cut(s) 1153, 1380
ScrFI CCNGG 2 cut(s) 84, 533
SduI GDGCHC 1 cut(s) 233
SfaNI GCATC 4 cut(s) 504, 917, 918, 1301
SfcI CTRYAG 1 cut(s) 1332
SinI GGWCC 2 cut(s) 951, 998
SmiMI CAYNNNNRTG 2 cut(s) 692, 783
SmlI CTYRAG 2 cut(s) 161, 1388
SmoI CTYRAG 2 cut(s) 161, 1388
Sse9I AATT 8 cut(s) 220, 307, 408, 424, 816, 1002, 1298, 1409
SsiI CCGC 3 cut(s) 381, 404, 918
SspMI CTAG 2 cut(s) 503, 831
StyD4I CCNGG 2 cut(s) 82, 531
StyI CCWWGG 6 cut(s) 237, 384, 596, 624, 947, 1167
TaaI ACNGT 2 cut(s) 1336, 1370
TaiI ACGT 2 cut(s) 1183, 1432
TaqI TCGA 5 cut(s) 66, 455, 839, 889, 1397
TaqII GACCGA 2 cut(s) 1330, 1394
TasI AATT 8 cut(s) 220, 307, 408, 424, 816, 1002, 1298, 1409
TatI WGTACW 1 cut(s) 848
TfiI GAWTC 4 cut(s) 367, 553, 836, 1040
Tru1I TTAA 7 cut(s) 423, 671, 737, 861, 981, 1233, 1259
Tru9I TTAA 7 cut(s) 423, 671, 737, 861, 981, 1233, 1259
TscAI CASTG 2 cut(s) 1227, 1258
TseFI GTSAC 3 cut(s) 1181, 1342, 1430
TseI GCWGC 4 cut(s) 137, 149, 152, 539
Tsp45I GTSAC 3 cut(s) 1181, 1342, 1430
TspDTI ATGAA 3 cut(s) 599, 699, 887
TspRI CASTG 2 cut(s) 1227, 1258
Van91I CCANNNNNTGG 1 cut(s) 1353
VpaK11BI GGWCC 2 cut(s) 951, 998
XapI RAATTY 1 cut(s) 307
XceI RCATGY 1 cut(s) 760
XmiI GTMKAC 2 cut(s) 324, 616
XspI CTAG 2 cut(s) 503, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.