FvH4_7g00214

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003709
Physical Location & Seq
Forward (+)
811293 .. 815337
4045 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g00214.t3

Sequence Viewer

Length: 1527 bp
ATGGCATTCGGATATACTGTGTTATTGTCCTTGCTGCTAGCTTTGGCTGCAGCTTCTAGCTTCTCCGTTGGCACTTCCCATGCCTACTATATCCCTTTTAACCGAAGCAGCTTTCCGAGTGATTTCCTTTTTGGTGCTGGTTCAGCTGCTTATCAGTCAGAAGGAGCAGCATTTCTCGATGGTAGGGGAGCTAGTATCTGGGATACCTTCGTTAGGAAACATCCAGAAAAGATATCGGATCATAGCACCGGAGACGTAGCTAATGACTTTTATCACCGCTATAAGGAGGATATAAAATTGATGAAGAAAATTGGGTTGGACACCTTCAGAATGTCAATTTCATGGACCAGAGTACTTCCCAAGGGAAAACTTGGCGGAGGAGTGAATCCACTTGGTGTCAAGTTCTACAACAATGTTTTCAACGAGCTCTTGGCCAATGGTATCAAGCCCTTCGTGACTTTGCTCCATTATGATCCTCCCCAAGCACTTGAAGATCTGTATGGCGGGTTCTTAAGCCCTAATATAATAAATGATTATGGAGATTATGTGGATTTCTGCTTCAAGACATTTGGTGATCGAGTGAAGCACTGGGTCACAATGAATGAACCTAATGGATTTGCTATTAGTGGCTACGGCGGCGGAACCTTTGCACCGGGGCGGTGTTCTAACTACGAGGGAAATTGCACTTCCGGTAACTCAGCGACTGAATCCTACACTGTGGCACACCATTTGCTTCTCGCTCATTCAGCTGCTGTCAAAATATACAAGGACAAATATCAAGCCTCCCAAAAGGGACAGATCGGGATCACAGTAGTGACTCACTGGTGGAAACCAAAGTACCCAGCACAACTGGCAAGCCGCACGGCCACCCTTAGATCACTTGATTTCATGTTTGGATGGTTCGCCAATCCCATAGTTCATGGGGATTATCCGGAGGTCATGCGATCTATGGTCGGGAATCGATTGCCGAAATTTACAGAAGCCGAATCGAAGCAGATAAAAGGATCCCTGGACTTTCTCGGGTTGAATTACTACACATCATATTACACAGAAGATGGACCAGCTTCATCCAACCATAGCTGGACTTCAGACAGACAAGTCACTGCTTCCACTACTGATAAAGCTGGGGTTCTTATTGGTGCAGCGACTGATTTGGATTGGCTATACGTTTATCCAAAGGGGATTCGAGATGTTCTTTTGTATATAAAGGAAAAGTACAACAATCCCAACATTTTCATAACTGAGAATGGATATGCATATGGATATAATGCCTCGACGCCTATTGAAGAAGCTCGTAAAGACAACCTGAGGATAAGATACCATCACGACCATCTATGGTATCTCTTGAAAGCTATTAAGGATGGAGTTCAAGTGAAGGGATACTACGCATGGTCGTTTTTTGATGACTTCGAATGGGATGCTGGTTACACAGTTGGGTTTGGCCTAACATTGGTTGATGTCAAGGATAATCTGAAAAGATACCTCAAGTATTCTGCCTATTGGTACAAGATGTTCCTCCTACACTGA

Protein Analysis

509

Amino Acids

57.32

Weight (kDa)

8.09

Isoelectric Point (pI)

24.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 1 - 404 3.2e-122 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1097
AccIII TCCGGA 1 cut(s) 931
AciI CCGC 7 cut(s) 277, 375, 504, 636, 639, 658, 859
AclWI GGATC 5 cut(s) 246, 467, 812, 999, 1012
AcoI YGGCCR 2 cut(s) 432, 864
AcsI RAATTY 1 cut(s) 971
AcuI CTGAAG 2 cut(s) 310, 1071
AcyI GRCGYC 1 cut(s) 1277
AdeI CACNNNGTG 1 cut(s) 395
AfaI GTAC 4 cut(s) 354, 839, 1217, 1505
AfiI CCNNNNNNNGG 3 cut(s) 213, 283, 1450
AflII CTTAAG 1 cut(s) 511
AgsI TTSAA 7 cut(s) 421, 491, 562, 1027, 1286, 1348, 1370
AjnI CCWGG 1 cut(s) 1008
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
AleI CACNNNNGTG 1 cut(s) 812
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 1 cut(s) 246
AlwI GGATC 5 cut(s) 246, 467, 812, 999, 1012
AlwNI CAGNNNCTG 3 cut(s) 704, 752, 1148
Ama87I CYCGRG 1 cut(s) 1019
Aor13HI TCCGGA 1 cut(s) 931
AoxI GGCC 3 cut(s) 432, 864, 1441
ApeKI GCWGC 8 cut(s) 34, 47, 50, 108, 146, 167, 749, 1142
ApoI RAATTY 1 cut(s) 971
AspS9I GGNCC 2 cut(s) 345, 1058
AsuC2I CCSGG 1 cut(s) 654
AsuHPI GGTGA 2 cut(s) 266, 584
AsuII TTCGAA 1 cut(s) 1410
AsuNHI GCTAGC 1 cut(s) 37
AvaI CYCGRG 1 cut(s) 1019
AvaII GGWCC 2 cut(s) 345, 1058
AxyI CCTNAGG 1 cut(s) 1307
BaeI ACNNNNGTAYC 4 cut(s) 195, 228, 821, 854
BalI TGGCCA 1 cut(s) 434
BamHI GGATCC 1 cut(s) 1004
BanII GRGCYC 1 cut(s) 429
BarI GAAGNNNNNNTAC 2 cut(s) 1367, 1399
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 8 cut(s) 21, 34, 62, 120, 133, 179, 736, 1154
BccI CCATC 6 cut(s) 173, 891, 1049, 1329, 1338, 1355
BceAI ACGGC 2 cut(s) 649, 879
BcgI CGANNNNNNTGC 2 cut(s) 1400, 1434
BciT130I CCWGG 1 cut(s) 1010
BciVI GTATCC 2 cut(s) 196, 1373
BcnI CCSGG 1 cut(s) 654
BcoDI GTCTC 1 cut(s) 246
BfaI CTAG 3 cut(s) 38, 57, 192
BfmI CTRYAG 1 cut(s) 48
BfrI CTTAAG 1 cut(s) 511
BfuI GTATCC 2 cut(s) 196, 1373
BglII AGATCT 1 cut(s) 493
BmcAI AGTACT 1 cut(s) 354
Bme1390I CCNGG 2 cut(s) 654, 1010
Bme18I GGWCC 2 cut(s) 345, 1058
BmeT110I CYCGRG 1 cut(s) 1019
BmgT120I GGNCC 2 cut(s) 345, 1058
BmiI GGNNCC 2 cut(s) 643, 1006
BmrFI CCNGG 2 cut(s) 654, 1010
BmrI ACTGGG 1 cut(s) 598
BmsI GCATC 1 cut(s) 1408
BmtI GCTAGC 1 cut(s) 41
BmuI ACTGGG 1 cut(s) 598
Bpu14I TTCGAA 1 cut(s) 1410
BpuEI CTTGAG 1 cut(s) 1469
BpuMI CCSGG 1 cut(s) 654
Bsa29I ATCGAT 1 cut(s) 961
BsaBI GATNNNNATC 1 cut(s) 803
BsaHI GRCGYC 1 cut(s) 1277
BsaJI CCNNGG 3 cut(s) 360, 653, 1008
BsaWI WCCGGW 3 cut(s) 248, 689, 931
BsaXI ACNNNNNCTCC 4 cut(s) 531, 561, 1356, 1386
Bsc4I CCNNNNNNNGG 3 cut(s) 213, 283, 1450
Bse1I ACTGG 3 cut(s) 593, 827, 855
Bse21I CCTNAGG 1 cut(s) 1307
Bse8I GATNNNNATC 1 cut(s) 803
BseAI TCCGGA 1 cut(s) 931
BseBI CCWGG 1 cut(s) 1010
BseCI ATCGAT 1 cut(s) 961
BseDI CCNNGG 3 cut(s) 360, 653, 1008
BseGI GGATG 5 cut(s) 220, 902, 1067, 1366, 1423
BseJI GATNNNNATC 1 cut(s) 803
BseLI CCNNNNNNNGG 3 cut(s) 213, 283, 1450
BseMII CTCAG 3 cut(s) 711, 1233, 1298
BseNI ACTGG 3 cut(s) 593, 827, 855
BseRI GAGGAG 1 cut(s) 393
BseXI GCAGC 8 cut(s) 21, 34, 62, 120, 133, 179, 736, 1154
BseYI CCCAGC 2 cut(s) 841, 1124
BsgI GTGCAG 1 cut(s) 1161
BshFI GGCC 3 cut(s) 434, 866, 1443
BshVI ATCGAT 1 cut(s) 961
BsiHKAI GWGCWC 1 cut(s) 429
BsiHKCI CYCGRG 1 cut(s) 1019
BsiSI CCGG 4 cut(s) 249, 653, 690, 932
BslFI GGGAC 1 cut(s) 807
BslI CCNNNNNNNGG 3 cut(s) 213, 283, 1450
BsmAI GTCTC 1 cut(s) 246
BsmBI CGTCTC 1 cut(s) 246
BsmFI GGGAC 1 cut(s) 807
BsmI GAATGC 1 cut(s) 5
BsnI GGCC 3 cut(s) 434, 866, 1443
BsoBI CYCGRG 1 cut(s) 1019
Bsp119I TTCGAA 1 cut(s) 1410
Bsp1286I GDGCHC 1 cut(s) 429
Bsp13I TCCGGA 1 cut(s) 931
Bsp143I GATC 9 cut(s) 238, 472, 493, 574, 798, 804, 875, 944, 1004
BspACI CCGC 7 cut(s) 277, 375, 504, 636, 639, 658, 859
BspANI GGCC 3 cut(s) 434, 866, 1443
BspCNI CTCAG 3 cut(s) 710, 1234, 1299
BspDI ATCGAT 1 cut(s) 961
BspEI TCCGGA 1 cut(s) 931
BspLI GGNNCC 2 cut(s) 643, 1006
BspMAI CTGCAG 1 cut(s) 52
BspOI GCTAGC 1 cut(s) 41
BspPI GGATC 5 cut(s) 246, 467, 812, 999, 1012
BspT104I TTCGAA 1 cut(s) 1410
BspTI CTTAAG 1 cut(s) 511
BsrI ACTGG 3 cut(s) 593, 827, 855
BssECI CCNNGG 3 cut(s) 360, 653, 1008
BssMI GATC 9 cut(s) 238, 472, 493, 574, 798, 804, 875, 944, 1004
BssNI GRCGYC 1 cut(s) 1277
BssT1I CCWWGG 1 cut(s) 360
Bst2UI CCWGG 1 cut(s) 1010
Bst4CI ACNGT 4 cut(s) 19, 718, 811, 1432
BstACI GRCGYC 1 cut(s) 1277
BstAFI CTTAAG 1 cut(s) 511
BstBI TTCGAA 1 cut(s) 1410
BstC8I GCNNGC 2 cut(s) 39, 856
BstDEI CTNAG 4 cut(s) 697, 872, 1242, 1307
BstENI CCTNNNNNAGG 1 cut(s) 211
BstF5I GGATG 5 cut(s) 220, 902, 1067, 1366, 1423
BstKTI GATC 9 cut(s) 241, 475, 496, 577, 801, 807, 878, 947, 1007
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 9 cut(s) 238, 472, 493, 574, 798, 804, 875, 944, 1004
BstMWI GCNNNNNNNGC 5 cut(s) 47, 143, 636, 746, 851
BstNI CCWGG 1 cut(s) 1010
BstSCI CCNGG 2 cut(s) 652, 1008
BstSFI CTRYAG 1 cut(s) 48
BstV1I GCAGC 8 cut(s) 21, 34, 62, 120, 133, 179, 736, 1154
BstX2I RGATCY 2 cut(s) 493, 1004
BstYI RGATCY 2 cut(s) 493, 1004
Bsu15I ATCGAT 1 cut(s) 961
Bsu36I CCTNAGG 1 cut(s) 1307
BsuI GTATCC 2 cut(s) 196, 1373
BsuRI GGCC 3 cut(s) 434, 866, 1443
BsuTUI ATCGAT 1 cut(s) 961
BtsCI GGATG 5 cut(s) 220, 902, 1067, 1366, 1423
BtsI GCAGTG 1 cut(s) 1101
BtsIMutI CAGTG 5 cut(s) 586, 714, 820, 1101, 1522
Cac8I GCNNGC 2 cut(s) 39, 856
CaiI CAGNNNCTG 3 cut(s) 704, 752, 1148
Cfr13I GGNCC 2 cut(s) 345, 1058
ClaI ATCGAT 1 cut(s) 961
CseI GACGC 1 cut(s) 1285
Csp6I GTAC 4 cut(s) 353, 838, 1216, 1504
CviAII CATG 6 cut(s) 80, 342, 889, 920, 940, 1389
CviQI GTAC 4 cut(s) 353, 838, 1216, 1504
DdeI CTNAG 4 cut(s) 697, 872, 1242, 1307
DpnI GATC 9 cut(s) 240, 474, 495, 576, 800, 806, 877, 946, 1006
DpnII GATC 9 cut(s) 238, 472, 493, 574, 798, 804, 875, 944, 1004
DraIII CACNNNGTG 1 cut(s) 395
DrdI GACNNNNNNGTC 1 cut(s) 1097
DseDI GACNNNNNNGTC 1 cut(s) 1097
EaeI YGGCCR 2 cut(s) 432, 864
EciI GGCGGA 2 cut(s) 390, 654
Ecl136II GAGCTC 1 cut(s) 427
Eco130I CCWWGG 1 cut(s) 360
Eco24I GRGCYC 1 cut(s) 429
Eco32I GATATC 1 cut(s) 234
Eco47I GGWCC 2 cut(s) 345, 1058
Eco53kI GAGCTC 1 cut(s) 427
Eco57I CTGAAG 2 cut(s) 310, 1071
Eco81I CCTNAGG 1 cut(s) 1307
Eco88I CYCGRG 1 cut(s) 1019
EcoICRI GAGCTC 1 cut(s) 427
EcoNI CCTNNNNNAGG 1 cut(s) 211
EcoRII CCWGG 1 cut(s) 1008
EcoRV GATATC 1 cut(s) 234
EcoT14I CCWWGG 1 cut(s) 360
EcoT22I ATGCAT 1 cut(s) 1258
EcoT38I GRGCYC 1 cut(s) 429
ErhI CCWWGG 1 cut(s) 360
Esp3I CGTCTC 1 cut(s) 246
FaeI CATG 6 cut(s) 83, 345, 892, 923, 943, 1392
FaqI GGGAC 1 cut(s) 807
FatI CATG 6 cut(s) 79, 341, 888, 919, 939, 1388
FauI CCCGC 1 cut(s) 497
FauNDI CATATG 1 cut(s) 1258
FokI GGATG 5 cut(s) 207, 909, 1054, 1373, 1430
FriOI GRGCYC 1 cut(s) 429
FspBI CTAG 3 cut(s) 38, 57, 192
GsaI CCCAGC 2 cut(s) 845, 1128
HaeIII GGCC 3 cut(s) 434, 866, 1443
HapII CCGG 4 cut(s) 249, 653, 690, 932
HgaI GACGC 1 cut(s) 1285
Hin1I GRCGYC 1 cut(s) 1277
Hin1II CATG 6 cut(s) 83, 345, 892, 923, 943, 1392
HinfI GANTC 6 cut(s) 385, 707, 817, 958, 986, 1183
HpaII CCGG 4 cut(s) 249, 653, 690, 932
HphI GGTGA 2 cut(s) 266, 584
Hpy188I TCNGA 7 cut(s) 11, 117, 160, 238, 329, 1090, 1473
Hpy99I CGWCG 1 cut(s) 1279
HpyAV CCTTC 5 cut(s) 155, 217, 334, 460, 1369
HpyCH4III ACNGT 4 cut(s) 19, 718, 811, 1432
HpyCH4IV ACGT 2 cut(s) 255, 1167
HpyCH4V TGCA 5 cut(s) 50, 650, 684, 1142, 1256
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 143, 636, 746, 851
HpyF3I CTNAG 4 cut(s) 697, 872, 1242, 1307
HpySE526I ACGT 2 cut(s) 255, 1167
Hsp92I GRCGYC 1 cut(s) 1277
Hsp92II CATG 6 cut(s) 83, 345, 892, 923, 943, 1392
Kpn2I TCCGGA 1 cut(s) 931
Kzo9I GATC 9 cut(s) 238, 472, 493, 574, 798, 804, 875, 944, 1004
LmnI GCTCC 3 cut(s) 164, 188, 468
Lsp1109I GCAGC 8 cut(s) 21, 34, 62, 120, 133, 179, 736, 1154
LweI GCATC 1 cut(s) 1408
MaeI CTAG 3 cut(s) 38, 57, 192
MaeII ACGT 2 cut(s) 255, 1167
MaeIII GTNAC 6 cut(s) 454, 592, 692, 814, 1099, 1424
MalI GATC 9 cut(s) 240, 474, 495, 576, 800, 806, 877, 946, 1006
MboI GATC 9 cut(s) 238, 472, 493, 574, 798, 804, 875, 944, 1004
MboII GAAGA 4 cut(s) 316, 503, 1064, 1298
MflI RGATCY 2 cut(s) 493, 1004
MhlI GDGCHC 1 cut(s) 429
MlsI TGGCCA 1 cut(s) 434
MluCI AATT 6 cut(s) 296, 309, 336, 679, 971, 1027
MluNI TGGCCA 1 cut(s) 434
MlyI GAGTC 1 cut(s) 811
MmeI TCCRAC 2 cut(s) 297, 1095
Mox20I TGGCCA 1 cut(s) 434
Mph1103I ATGCAT 1 cut(s) 1258
MroI TCCGGA 1 cut(s) 931
MscI TGGCCA 1 cut(s) 434
MseI TTAA 3 cut(s) 99, 512, 1356
MslI CAYNNNNRTG 1 cut(s) 812
Msp20I TGGCCA 1 cut(s) 434
MspA1I CMGCKG 2 cut(s) 146, 749
MspCI CTTAAG 1 cut(s) 511
MspI CCGG 4 cut(s) 249, 653, 690, 932
MspR9I CCNGG 2 cut(s) 654, 1010
Mva1269I GAATGC 1 cut(s) 5
MvaI CCWGG 1 cut(s) 1010
MwoI GCNNNNNNNGC 5 cut(s) 47, 143, 636, 746, 851
NciI CCSGG 1 cut(s) 654
NdeI CATATG 1 cut(s) 1258
NdeII GATC 9 cut(s) 238, 472, 493, 574, 798, 804, 875, 944, 1004
NheI GCTAGC 1 cut(s) 37
NlaIII CATG 6 cut(s) 83, 345, 892, 923, 943, 1392
NlaIV GGNNCC 2 cut(s) 643, 1006
NmuCI GTSAC 4 cut(s) 454, 592, 814, 1099
NsiI ATGCAT 1 cut(s) 1258
NspV TTCGAA 1 cut(s) 1410
OliI CACNNNNGTG 1 cut(s) 812
PctI GAATGC 1 cut(s) 5
PfeI GAWTC 5 cut(s) 385, 707, 958, 986, 1183
PleI GAGTC 1 cut(s) 811
PpsI GAGTC 1 cut(s) 811
Psp124BI GAGCTC 1 cut(s) 429
Psp6I CCWGG 1 cut(s) 1008
PspFI CCCAGC 2 cut(s) 841, 1124
PspGI CCWGG 1 cut(s) 1008
PspN4I GGNNCC 2 cut(s) 643, 1006
PspPI GGNCC 2 cut(s) 345, 1058
PsrI GAACNNNNNNTAC 3 cut(s) 491, 523, 1496
PstI CTGCAG 1 cut(s) 52
PstNI CAGNNNCTG 3 cut(s) 704, 752, 1148
PsuI RGATCY 2 cut(s) 493, 1004
PvuII CAGCTG 2 cut(s) 146, 749
RsaI GTAC 4 cut(s) 354, 839, 1217, 1505
RsaNI GTAC 4 cut(s) 353, 838, 1216, 1504
RseI CAYNNNNRTG 1 cut(s) 812
SacI GAGCTC 1 cut(s) 429
SaqAI TTAA 3 cut(s) 99, 512, 1356
Sau3AI GATC 9 cut(s) 238, 472, 493, 574, 798, 804, 875, 944, 1004
Sau96I GGNCC 2 cut(s) 345, 1058
ScaI AGTACT 1 cut(s) 354
SchI GAGTC 1 cut(s) 811
ScrFI CCNGG 2 cut(s) 654, 1010
SduI GDGCHC 1 cut(s) 429
SfaNI GCATC 1 cut(s) 1408
SfcI CTRYAG 1 cut(s) 48
SfuI TTCGAA 1 cut(s) 1410
SinI GGWCC 2 cut(s) 345, 1058
SmiMI CAYNNNNRTG 1 cut(s) 812
SmlI CTYRAG 2 cut(s) 511, 1484
SmoI CTYRAG 2 cut(s) 511, 1484
Sse9I AATT 6 cut(s) 296, 309, 336, 679, 971, 1027
SsiI CCGC 7 cut(s) 277, 375, 504, 636, 639, 658, 859
SspMI CTAG 3 cut(s) 38, 57, 192
SstI GAGCTC 1 cut(s) 429
StyD4I CCNGG 2 cut(s) 652, 1008
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 4 cut(s) 19, 718, 811, 1432
TaiI ACGT 2 cut(s) 258, 1170
TaqI TCGA 7 cut(s) 177, 577, 961, 989, 1186, 1274, 1410
TasI AATT 6 cut(s) 296, 309, 336, 679, 971, 1027
TatI WGTACW 2 cut(s) 352, 1215
TauI GCSGC 2 cut(s) 639, 861
TfiI GAWTC 5 cut(s) 385, 707, 958, 986, 1183
Tru1I TTAA 3 cut(s) 99, 512, 1356
Tru9I TTAA 3 cut(s) 99, 512, 1356
TscAI CASTG 4 cut(s) 593, 721, 827, 1108
TseFI GTSAC 4 cut(s) 454, 592, 814, 1099
TseI GCWGC 8 cut(s) 34, 47, 50, 108, 146, 167, 749, 1142
Tsp45I GTSAC 4 cut(s) 454, 592, 814, 1099
TspDTI ATGAA 8 cut(s) 317, 330, 614, 618, 877, 908, 1056, 1225
TspGWI ACGGA 1 cut(s) 55
TspRI CASTG 4 cut(s) 593, 721, 827, 1108
Vha464I CTTAAG 1 cut(s) 511
VpaK11BI GGWCC 2 cut(s) 345, 1058
XagI CCTNNNNNAGG 1 cut(s) 211
XapI RAATTY 1 cut(s) 971
XspI CTAG 3 cut(s) 38, 57, 192
ZrmI AGTACT 1 cut(s) 354
Zsp2I ATGCAT 1 cut(s) 1258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.