Rw1G000340

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0003709
Physical Location & Seq
Forward (+)
467752 .. 471397
3646 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G000340.1

Sequence Viewer

Length: 1563 bp
ATGGGTGGTCGTCCTCTGCTCTTCTTCATCTTGATCTTTATCTGCCTCATACTCACTGCTCTTGTTGGCTCATCAGCAAGCAATGAGGCGGCTGCTGCTGCTGCTGCTCCTCGTCATTATTCTATTCCATTCAATAGAAGTAGCTTCCCAGCTAGTTTCATCTTCGGAGCTGGTTCTGCTGCTTATCAGACTGAAGGAGCAGCACATATCCATGGAAAGGGACCAAGCATATGGGACACTTTCACTACAAAACATCAAGAAAAAATAACTGATGGTAGCAACGGGGATGTAGCAGATGACTTTTACCATCGTTATGAGGAAGATGTGAAATTGATGAAAAAAGTCGGTTTAGACTCCTTCAGATTTTCAATCTCATGGTCAAGATTACTACCTAAGGGAAAAGTCAAAGGTGGAGTGAACCAAGAAGGCGTAAAATTCTACAACAATCTTATAAATGAGCTCTTGTCGAATGGAATAATACCTTTTGTGACGCTTTTCCACTGGGATCTACCACAGGCTCTTGAAGATGAGTATGGTGGATTTTTAAATCATAAGATCGTTGGTGATTTTCAAGATTATGCCGATTTTTGTTTCAAAACATTTGGTGACCGAGTTAAGCATTGGATCACTCTGAACGAGCCAGTTACATACTGTGTCAACGGGTACAATGGTGGAACCAATGCACCTGGTCGATGCTCTAACTATGTCGGAAATTGCACCGCCGGTAACTCTGCCACAGAGCCTTACATTGTAGGCCATCATCTGCTGCTTGCTCACGCGCACTCTGCAAATTTATACAAGAAGAAGTATCAGGCTTCTCAAAAAGGGCAAATCGGGATTTCGGTTGTGACATACTGGTATTTAGCAAAATCGGAAACTGCTGCAAGCAAGAGGGCAGCTTCTAGGTCTCTTGATTTTGTTTTTGGATGGTTTGCTCATCCCGTTACATTCGGTGACTATCCTCAAACCATGAGAGCTTCTGTGGGTGATCGGTTGCCTAAATTCAGTAAAGAAGAAACTAAGCTCCTAAAAGGATCTCTAGATTTCCTTGGTGTCAATTACTACACAAGTAGTTATGCAGATGTTTCCTCACTCTCCACTAGTAATGTCAACCAAAGTTTCTTCGGAGATATGCATACTTCTCTCTCTACTGAGAAAAATGGGGTTCCTTTGGGTTCAGCGACTGCTTTGAGCTGGCTTTACATCTATCCAAAGGGAATTCGAGAGCTTATGTTATACATAAAAGAAAAGTACAATGATCCCGAGATTTACATTACTGAAAATGGTGTTGGTGAGGCAAATAATAGCTCCCAGCCCCTCAACGATGCCCTCAAGGATAGTACAAGGATAAGATACCATTCTCTCCATCTTTCATATCTTTTGAAGTCCATCAAGGAGGGGGTGAAGGTGAGGGGATACTTCGCATGGTCATTTCTTGATGTTTATGAATGGGATGCAGGCTACACAGTTCGATTTGGACTCACCTACGTCGATTACAAGAATAACTTGAAGAGATATCTCAAGTACTCTGCATACTGGTTCAAGATGTTCCTCCTCAAATGA

Protein Analysis

520

Amino Acids

58.35

Weight (kDa)

8.79

Isoelectric Point (pI)

25.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 47 - 517 5.3e-156 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 452
AccII CGCG 1 cut(s) 779
AciI CCGC 2 cut(s) 89, 720
AclWI GGATC 4 cut(s) 513, 632, 1042, 1253
AcsI RAATTY 4 cut(s) 434, 790, 1001, 1218
AcuI CTGAAG 2 cut(s) 213, 343
AfaI GTAC 4 cut(s) 665, 1253, 1342, 1526
AfiI CCNNNNNNNGG 1 cut(s) 217
AgsI TTSAA 8 cut(s) 133, 369, 524, 572, 595, 1384, 1510, 1543
AhlI ACTAGT 1 cut(s) 1100
AjnI CCWGG 1 cut(s) 685
AjuI GAANNNNNNNTTGG 2 cut(s) 1272, 1304
Alw21I GWGCWC 1 cut(s) 462
Alw26I GTCTC 1 cut(s) 912
AlwI GGATC 4 cut(s) 513, 632, 1042, 1253
AlwNI CAGNNNCTG 1 cut(s) 1184
Ama87I CYCGRG 1 cut(s) 1262
AoxI GGCC 1 cut(s) 754
ApoI RAATTY 4 cut(s) 434, 790, 1001, 1218
AspLEI GCGC 1 cut(s) 781
AspS9I GGNCC 1 cut(s) 221
AsuHPI GGTGA 8 cut(s) 575, 617, 965, 998, 1304, 1414, 1420, 1474
AvaI CYCGRG 1 cut(s) 1262
AvaII GGWCC 1 cut(s) 221
AxyI CCTNAGG 1 cut(s) 393
BaeI ACNNNNGTAYC 2 cut(s) 655, 688
BanII GRGCYC 1 cut(s) 462
Bbv12I GWGCWC 1 cut(s) 462
BccI CCATC 6 cut(s) 266, 315, 765, 921, 1374, 1397
BciT130I CCWGG 1 cut(s) 687
BciVI GTATCC 1 cut(s) 1409
BcoDI GTCTC 1 cut(s) 912
BcuI ACTAGT 1 cut(s) 1100
BfaI CTAG 4 cut(s) 153, 903, 1040, 1101
BfuI GTATCC 1 cut(s) 1409
BmcAI AGTACT 1 cut(s) 1526
Bme1390I CCNGG 1 cut(s) 687
Bme18I GGWCC 1 cut(s) 221
BmeT110I CYCGRG 1 cut(s) 1262
BmgT120I GGNCC 1 cut(s) 221
BmiI GGNNCC 3 cut(s) 222, 676, 1167
BmrFI CCNGG 1 cut(s) 687
BmrI ACTGGG 1 cut(s) 511
BmsI GCATC 3 cut(s) 683, 1315, 1444
BmuI ACTGGG 1 cut(s) 511
BpuEI CTTGAG 2 cut(s) 1316, 1505
BsaBI GATNNNNATC 1 cut(s) 38
BsaI GGTCTC 1 cut(s) 912
BsaJI CCNNGG 2 cut(s) 211, 1048
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse118I RCCGGY 1 cut(s) 722
Bse1I ACTGG 4 cut(s) 506, 641, 860, 1541
Bse21I CCTNAGG 1 cut(s) 393
Bse3DI GCAATG 1 cut(s) 88
Bse8I GATNNNNATC 1 cut(s) 38
BseBI CCWGG 1 cut(s) 687
BseDI CCNNGG 2 cut(s) 211, 1048
BseGI GGATG 4 cut(s) 292, 932, 937, 1459
BseJI GATNNNNATC 1 cut(s) 38
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMI GCAATG 1 cut(s) 88
BseMII CTCAG 1 cut(s) 1143
BseNI ACTGG 4 cut(s) 506, 641, 860, 1541
BseRI GAGGAG 2 cut(s) 99, 1544
BseYI CCCAGC 2 cut(s) 148, 1311
Bsh1236I CGCG 1 cut(s) 779
BshFI GGCC 1 cut(s) 756
BsiHKAI GWGCWC 1 cut(s) 462
BsiHKCI CYCGRG 1 cut(s) 1262
BsiSI CCGG 1 cut(s) 723
BslFI GGGAC 2 cut(s) 234, 248
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 1 cut(s) 912
BsmFI GGGAC 2 cut(s) 234, 248
BsnI GGCC 1 cut(s) 756
Bso31I GGTCTC 1 cut(s) 912
BsoBI CYCGRG 1 cut(s) 1262
Bsp1286I GDGCHC 1 cut(s) 462
Bsp143I GATC 7 cut(s) 33, 505, 555, 624, 988, 1034, 1258
Bsp19I CCATGG 1 cut(s) 211
BspACI CCGC 2 cut(s) 89, 720
BspANI GGCC 1 cut(s) 756
BspCNI CTCAG 1 cut(s) 1144
BspFNI CGCG 1 cut(s) 779
BspLI GGNNCC 3 cut(s) 222, 676, 1167
BspPI GGATC 4 cut(s) 513, 632, 1042, 1253
BspQI GCTCTTC 1 cut(s) 26
BspTNI GGTCTC 1 cut(s) 912
BsrDI GCAATG 1 cut(s) 88
BsrFI RCCGGY 1 cut(s) 722
BsrI ACTGG 4 cut(s) 506, 641, 860, 1541
BssAI RCCGGY 1 cut(s) 722
BssECI CCNNGG 2 cut(s) 211, 1048
BssMI GATC 7 cut(s) 33, 505, 555, 624, 988, 1034, 1258
BssT1I CCWWGG 2 cut(s) 211, 1048
Bst2UI CCWGG 1 cut(s) 687
Bst4CI ACNGT 2 cut(s) 653, 1468
Bst6I CTCTTC 2 cut(s) 26, 1505
BstC8I GCNNGC 5 cut(s) 79, 771, 886, 1196, 1459
BstDEI CTNAG 3 cut(s) 393, 1020, 1152
BstDSI CCRYGG 1 cut(s) 211
BstEII GGTNACC 1 cut(s) 605
BstF5I GGATG 4 cut(s) 292, 932, 937, 1459
BstFNI CGCG 1 cut(s) 779
BstHHI GCGC 1 cut(s) 781
BstKTI GATC 7 cut(s) 36, 508, 558, 627, 991, 1037, 1261
BstMAI GTCTC 1 cut(s) 912
BstMBI GATC 7 cut(s) 33, 505, 555, 624, 988, 1034, 1258
BstMWI GCNNNNNNNGC 6 cut(s) 95, 98, 101, 104, 176, 785
BstNI CCWGG 1 cut(s) 687
BstPI GGTNACC 1 cut(s) 605
BstSCI CCNGG 1 cut(s) 685
BstUI CGCG 1 cut(s) 779
BstX2I RGATCY 2 cut(s) 505, 1034
BstXI CCANNNNNNTGG 1 cut(s) 231
BstYI RGATCY 2 cut(s) 505, 1034
Bsu36I CCTNAGG 1 cut(s) 393
BsuI GTATCC 1 cut(s) 1409
BsuRI GGCC 1 cut(s) 756
BtgI CCRYGG 1 cut(s) 211
BtsCI GGATG 4 cut(s) 292, 932, 937, 1459
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 2 cut(s) 54, 499
Cac8I GCNNGC 5 cut(s) 79, 771, 886, 1196, 1459
CaiI CAGNNNCTG 1 cut(s) 1184
CfoI GCGC 1 cut(s) 781
Cfr10I RCCGGY 1 cut(s) 722
Cfr13I GGNCC 1 cut(s) 221
CseI GACGC 1 cut(s) 499
CsiI ACCWGGT 1 cut(s) 685
Csp6I GTAC 4 cut(s) 664, 1252, 1341, 1525
CviAII CATG 4 cut(s) 212, 375, 970, 1425
CviQI GTAC 4 cut(s) 664, 1252, 1341, 1525
DdeI CTNAG 3 cut(s) 393, 1020, 1152
DpnI GATC 7 cut(s) 35, 507, 557, 626, 990, 1036, 1260
DpnII GATC 7 cut(s) 33, 505, 555, 624, 988, 1034, 1258
DraI TTTAAA 1 cut(s) 546
Eam1104I CTCTTC 2 cut(s) 26, 1505
EarI CTCTTC 2 cut(s) 26, 1505
Ecl136II GAGCTC 1 cut(s) 460
Eco130I CCWWGG 2 cut(s) 211, 1048
Eco24I GRGCYC 1 cut(s) 462
Eco31I GGTCTC 1 cut(s) 912
Eco32I GATATC 1 cut(s) 1517
Eco47I GGWCC 1 cut(s) 221
Eco53kI GAGCTC 1 cut(s) 460
Eco57I CTGAAG 2 cut(s) 213, 343
Eco81I CCTNAGG 1 cut(s) 393
Eco88I CYCGRG 1 cut(s) 1262
Eco91I GGTNACC 1 cut(s) 605
EcoICRI GAGCTC 1 cut(s) 460
EcoO65I GGTNACC 1 cut(s) 605
EcoRI GAATTC 1 cut(s) 1218
EcoRII CCWGG 1 cut(s) 685
EcoRV GATATC 1 cut(s) 1517
EcoT14I CCWWGG 2 cut(s) 211, 1048
EcoT22I ATGCAT 1 cut(s) 1137
EcoT38I GRGCYC 1 cut(s) 462
ErhI CCWWGG 2 cut(s) 211, 1048
FaeI CATG 4 cut(s) 215, 378, 973, 1428
FalI AAGNNNNNCTT 2 cut(s) 1490, 1522
FaqI GGGAC 2 cut(s) 234, 248
FatI CATG 4 cut(s) 211, 374, 969, 1424
FauNDI CATATG 1 cut(s) 230
FokI GGATG 4 cut(s) 299, 924, 939, 1466
FriOI GRGCYC 1 cut(s) 462
FspBI CTAG 4 cut(s) 153, 903, 1040, 1101
GlaI GCGC 1 cut(s) 780
GsaI CCCAGC 2 cut(s) 152, 1315
HaeIII GGCC 1 cut(s) 756
HapII CCGG 1 cut(s) 723
HgaI GACGC 1 cut(s) 499
HhaI GCGC 1 cut(s) 781
Hin1II CATG 4 cut(s) 215, 378, 973, 1428
Hin6I GCGC 1 cut(s) 779
HinP1I GCGC 1 cut(s) 779
HincII GTYRAC 2 cut(s) 658, 1111
HindII GTYRAC 2 cut(s) 658, 1111
HinfI GANTC 2 cut(s) 353, 1479
HpaII CCGG 1 cut(s) 723
HphI GGTGA 8 cut(s) 575, 617, 965, 998, 1304, 1414, 1420, 1474
Hpy166II GTNNAC 3 cut(s) 418, 658, 1111
Hpy188I TCNGA 7 cut(s) 167, 189, 362, 633, 710, 874, 1127
Hpy8I GTNNAC 3 cut(s) 418, 658, 1111
Hpy99I CGWCG 1 cut(s) 1493
HpyAV CCTTC 4 cut(s) 188, 367, 419, 1399
HpyCH4III ACNGT 2 cut(s) 653, 1468
HpyCH4IV ACGT 1 cut(s) 1488
HpyCH4V TGCA 8 cut(s) 683, 717, 788, 884, 1079, 1135, 1457, 1532
HpyF10VI GCNNNNNNNGC 6 cut(s) 95, 98, 101, 104, 176, 785
HpyF3I CTNAG 3 cut(s) 393, 1020, 1152
HpySE526I ACGT 1 cut(s) 1488
Hsp92II CATG 4 cut(s) 215, 378, 973, 1428
HspAI GCGC 1 cut(s) 779
Kzo9I GATC 7 cut(s) 33, 505, 555, 624, 988, 1034, 1258
LguI GCTCTTC 1 cut(s) 26
LmnI GCTCC 5 cut(s) 112, 167, 197, 1029, 1313
LweI GCATC 3 cut(s) 683, 1315, 1444
MabI ACCWGGT 1 cut(s) 685
MaeI CTAG 4 cut(s) 153, 903, 1040, 1101
MaeII ACGT 1 cut(s) 1488
MaeIII GTNAC 7 cut(s) 487, 605, 643, 725, 847, 943, 953
MalI GATC 7 cut(s) 35, 507, 557, 626, 990, 1036, 1260
MboI GATC 7 cut(s) 33, 505, 555, 624, 988, 1034, 1258
MboII GAAGA 9 cut(s) 13, 16, 154, 332, 536, 814, 1025, 1114, 1522
MflI RGATCY 2 cut(s) 505, 1034
MhlI GDGCHC 1 cut(s) 462
MluCI AATT 7 cut(s) 329, 434, 712, 790, 1001, 1057, 1218
MlyI GAGTC 2 cut(s) 347, 1473
MmeI TCCRAC 1 cut(s) 688
Mph1103I ATGCAT 1 cut(s) 1137
MseI TTAA 2 cut(s) 545, 615
MslI CAYNNNNRTG 2 cut(s) 210, 312
MspI CCGG 1 cut(s) 723
MspR9I CCNGG 1 cut(s) 687
MvaI CCWGG 1 cut(s) 687
MvnI CGCG 1 cut(s) 779
MwoI GCNNNNNNNGC 6 cut(s) 95, 98, 101, 104, 176, 785
NcoI CCATGG 1 cut(s) 211
NdeI CATATG 1 cut(s) 230
NdeII GATC 7 cut(s) 33, 505, 555, 624, 988, 1034, 1258
NlaIII CATG 4 cut(s) 215, 378, 973, 1428
NlaIV GGNNCC 3 cut(s) 222, 676, 1167
NmuCI GTSAC 4 cut(s) 487, 605, 847, 953
NsiI ATGCAT 1 cut(s) 1137
PciSI GCTCTTC 1 cut(s) 26
PleI GAGTC 2 cut(s) 347, 1473
PpsI GAGTC 2 cut(s) 347, 1473
PsiI TTATAA 1 cut(s) 452
Psp124BI GAGCTC 1 cut(s) 462
Psp6I CCWGG 1 cut(s) 685
PspEI GGTNACC 1 cut(s) 605
PspFI CCCAGC 2 cut(s) 148, 1311
PspGI CCWGG 1 cut(s) 685
PspN4I GGNNCC 3 cut(s) 222, 676, 1167
PspPI GGNCC 1 cut(s) 221
PstNI CAGNNNCTG 1 cut(s) 1184
PsuI RGATCY 2 cut(s) 505, 1034
RsaI GTAC 4 cut(s) 665, 1253, 1342, 1526
RsaNI GTAC 4 cut(s) 664, 1252, 1341, 1525
RseI CAYNNNNRTG 2 cut(s) 210, 312
SacI GAGCTC 1 cut(s) 462
SapI GCTCTTC 1 cut(s) 26
SaqAI TTAA 2 cut(s) 545, 615
Sau3AI GATC 7 cut(s) 33, 505, 555, 624, 988, 1034, 1258
Sau96I GGNCC 1 cut(s) 221
ScaI AGTACT 1 cut(s) 1526
SchI GAGTC 2 cut(s) 347, 1473
ScrFI CCNGG 1 cut(s) 687
SduI GDGCHC 1 cut(s) 462
SexAI ACCWGGT 1 cut(s) 685
SfaNI GCATC 3 cut(s) 683, 1315, 1444
SinI GGWCC 1 cut(s) 221
SmiMI CAYNNNNRTG 2 cut(s) 210, 312
SmlI CTYRAG 2 cut(s) 1331, 1520
SmoI CTYRAG 2 cut(s) 1331, 1520
SpeI ACTAGT 1 cut(s) 1100
Sse9I AATT 7 cut(s) 329, 434, 712, 790, 1001, 1057, 1218
SsiI CCGC 2 cut(s) 89, 720
SspMI CTAG 4 cut(s) 153, 903, 1040, 1101
SstI GAGCTC 1 cut(s) 462
StyD4I CCNGG 1 cut(s) 685
StyI CCWWGG 2 cut(s) 211, 1048
TaaI ACNGT 2 cut(s) 653, 1468
TaiI ACGT 1 cut(s) 1491
TaqI TCGA 5 cut(s) 467, 691, 1222, 1471, 1491
TaqII GACCGA 1 cut(s) 624
TasI AATT 7 cut(s) 329, 434, 712, 790, 1001, 1057, 1218
TatI WGTACW 3 cut(s) 1251, 1340, 1524
TauI GCSGC 1 cut(s) 92
Tru1I TTAA 2 cut(s) 545, 615
Tru9I TTAA 2 cut(s) 545, 615
TscAI CASTG 2 cut(s) 61, 506
TseFI GTSAC 4 cut(s) 487, 605, 847, 953
Tsp45I GTSAC 4 cut(s) 487, 605, 847, 953
TspDTI ATGAA 5 cut(s) 16, 148, 350, 1362, 1461
TspRI CASTG 2 cut(s) 61, 506
VpaK11BI GGWCC 1 cut(s) 221
XapI RAATTY 4 cut(s) 434, 790, 1001, 1218
XbaI TCTAGA 1 cut(s) 1039
XspI CTAG 4 cut(s) 153, 903, 1040, 1101
ZrmI AGTACT 1 cut(s) 1526
Zsp2I ATGCAT 1 cut(s) 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.