MD07G1003400.v1.1

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003709
Physical Location & Seq
Forward (+)
334102 .. 341622
7521 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1003400.v1.1.491

Sequence Viewer

Length: 1590 bp
ATGTTCAATGCATTTGAGCGGCAGAAGAGAACCAGTTGTAGAAACATGTTGGGTACTAAAGGAGGACGTCTTCCTTTTTGCTTCATCCTCGTACTTTTTGCTTTGTTCGCAAGTGCTGCTGTGGAAGTTGAACCGAGTCATTATTCGATGCCATTTAATCGAACTAGCTTCCCACCTGGGTTCATCTTTGGAGCCGGTTTAGCTGCTTATCAGTCTGAAGGAGCAGCTCTTGTTCATGGAAAGGGACCAAGTATATGGGATACCTTCACTAGGGAACATCCAGAAAAAATTTCGGATGGTAGCAATGGGAATGTAGCCGATGATTTTTACAATCACTATAAGGAGGACATAAAATTGATAAAAAAGATCGGCTTGGACTCCTTCAGATTTTCTTTCTCATGGTCAAGAATACTACCTAATGGTACAATTAAAGGTGGAGTGAACCAAGAAGGAGTCAACTTCTACAACAATCTGATAGATGAGCTCTTATCCAATGGAATTGTGCCTTTTGTGACCCTTTTCCATTGGGATACACCACAAGCACTTGAAGATGAGTACGGTGGATTTTTAAGCCCTAAGATTGTGAATGATTTTCAAGATTATGCCAATTTTTGTTTCAAAACATTTGGGGATCGTGTTAAGCATTGGGTGACATTGAACGAGCCTGTTACATATTGTGTTGATGGATACAGTGGCGGTACTTATGCACCCGGTCGATGTTCAAACTATGTCGGAAATTGCACTGCTGGTAACTCTGCCACTGAGCCTTACATTGTTGGTCATCATCTACTACTTGCTCATGCACACGCTGTGAAGATATACAGGCACAAGTATCAGGCCTCTCAGAAAGGACAAATTGGGATTACGGTTGTGACATTCTGGTATGTAGCAAAATCTGAAACAGCTGCAAGTAAGAGGGCGGCCTCTAAGTCTCTTGATTGTATTTTCGGCTGGTTTACGCATCCTGTTACATTCGGTGAGTATCCCAAAACTATGAGGTCTGCGGTGGGAAATCGGTTGCCTAAATTCAGCGAAGCAGAATCAAAGCTTCTTAAAGGATCCTTGGATTTCCTTGGTGTCAATTATTACACGAGTTGTTATGCAGATGCTTCCTCACTCTCCACTGTTACAACTGTTAACCAAAGTTTCTATGGAGACTTGAATACTGCTCTCTCTACGGAGAAAAATGGGGTTCCTTTGGGCACACTGACTGCTTTGAGCTGGCTGTACATCTACCCAAGGGGAATTCGAGAGCTTATGCTGCATATAAAGGAAAATTACAACAACCCGGAGATTTACATTAGCGAAAATGGTGTTGGCGAGGCAAATAATAGCTCCATGCCTATCAAGACTGCCCTCAAGGATAGTACCAGGATTAGATACCATTATCAACATCTTCAATATCTTTCGAAAGCCATCAAGGAGGGCGTTAAGGTGAAGGGATACTTTGCATGGGCACTTCTGGATGTGTATGAATGGGACTCAGGCTACACTGTTCGATTTGGGTTCACATATATTGACTACAAAAATAATATGAAAAGGTACCTCAAGCACTCTGCATATTGGTTTAAGATGTTCCTCCTCAAATGA

Protein Analysis

530

Amino Acids

59.72

Weight (kDa)

8.86

Isoelectric Point (pI)

28.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 55 - 526 4.7e-152 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 70
Acc65I GGTACC 1 cut(s) 1542
AccB1I GGYRCC 1 cut(s) 1542
AccBSI CCGCTC 1 cut(s) 19
AciI CCGC 4 cut(s) 19, 696, 920, 1004
AclWI GGATC 3 cut(s) 639, 1053, 1066
AcsI RAATTY 3 cut(s) 288, 1025, 1245
AcuI CTGAAG 2 cut(s) 237, 367
AcyI GRCGYC 1 cut(s) 67
AdeI CACNNNGTG 1 cut(s) 811
AfaI GTAC 8 cut(s) 55, 93, 424, 557, 700, 1229, 1369, 1544
AfiI CCNNNNNNNGG 1 cut(s) 270
AflIII ACRYGT 1 cut(s) 45
AgsI TTSAA 9 cut(s) 7, 131, 548, 596, 619, 658, 723, 1162, 1400
AjnI CCWGG 2 cut(s) 175, 1370
AjuI GAANNNNNNNTTGG 4 cut(s) 599, 631, 1299, 1331
AluBI AGCT 9 cut(s) 168, 203, 227, 484, 905, 1048, 1221, 1255, 1335
AluI AGCT 9 cut(s) 168, 203, 227, 484, 905, 1048, 1221, 1255, 1335
Alw21I GWGCWC 1 cut(s) 486
Alw26I GTCTC 2 cut(s) 936, 1149
AlwI GGATC 3 cut(s) 639, 1053, 1066
AoxI GGCC 2 cut(s) 837, 921
ApeKI GCWGC 5 cut(s) 116, 203, 224, 905, 1261
ApoI RAATTY 3 cut(s) 288, 1025, 1245
Asp718I GGTACC 1 cut(s) 1542
AspS9I GGNCC 1 cut(s) 245
AsuC2I CCSGG 2 cut(s) 711, 1289
AsuHPI GGTGA 3 cut(s) 661, 989, 1447
AsuII TTCGAA 1 cut(s) 1409
AvaII GGWCC 1 cut(s) 245
BaeGI GKGCMC 2 cut(s) 1205, 1459
BamHI GGATCC 1 cut(s) 1058
BanI GGYRCC 1 cut(s) 1542
BanII GRGCYC 1 cut(s) 486
BauI CACGAG 1 cut(s) 1090
BbsI GAAGAC 1 cut(s) 62
Bbv12I GWGCWC 1 cut(s) 486
BbvI GCAGC 5 cut(s) 103, 190, 236, 892, 1248
BccI CCATC 3 cut(s) 290, 677, 1424
BciT130I CCWGG 2 cut(s) 177, 1372
BciVI GTATCC 5 cut(s) 253, 523, 680, 993, 1436
BcnI CCSGG 2 cut(s) 711, 1289
BcoDI GTCTC 2 cut(s) 936, 1149
BfaI CTAG 2 cut(s) 165, 270
BfuI GTATCC 5 cut(s) 253, 523, 680, 993, 1436
BisI GCNGC 7 cut(s) 20, 117, 204, 225, 906, 921, 1262
BlsI GCNGC 7 cut(s) 21, 118, 205, 226, 907, 922, 1263
Bme1390I CCNGG 4 cut(s) 177, 711, 1289, 1372
Bme18I GGWCC 1 cut(s) 245
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 5 cut(s) 193, 246, 1060, 1194, 1544
BmrFI CCNGG 4 cut(s) 177, 711, 1289, 1372
BmsI GCATC 3 cut(s) 138, 970, 1096
BpiI GAAGAC 1 cut(s) 62
Bpu14I TTCGAA 1 cut(s) 1409
BpuEI CTTGAG 2 cut(s) 1343, 1532
BpuMI CCSGG 2 cut(s) 711, 1289
BsaHI GRCGYC 1 cut(s) 67
BsaJI CCNNGG 4 cut(s) 176, 1062, 1072, 1238
Bsc4I CCNNNNNNNGG 1 cut(s) 270
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 1 cut(s) 33
Bse3DI GCAATG 1 cut(s) 310
BseBI CCWGG 2 cut(s) 177, 1372
BseDI CCNNGG 4 cut(s) 176, 1062, 1072, 1238
BseGI GGATG 5 cut(s) 84, 277, 301, 961, 1471
BseLI CCNNNNNNNGG 1 cut(s) 270
BseMI GCAATG 1 cut(s) 310
BseMII CTCAG 3 cut(s) 753, 857, 1497
BseNI ACTGG 1 cut(s) 33
BseRI GAGGAG 1 cut(s) 1571
BseSI GKGCMC 2 cut(s) 1205, 1459
BseXI GCAGC 5 cut(s) 103, 190, 236, 892, 1248
Bsh1285I CGRYCG 1 cut(s) 715
BshFI GGCC 2 cut(s) 839, 923
BshNI GGYRCC 1 cut(s) 1542
BsiEI CGRYCG 1 cut(s) 715
BsiHKAI GWGCWC 1 cut(s) 486
BsiSI CCGG 3 cut(s) 195, 711, 1289
BslFI GGGAC 2 cut(s) 258, 1493
BslI CCNNNNNNNGG 1 cut(s) 270
BsmAI GTCTC 2 cut(s) 936, 1149
BsmFI GGGAC 2 cut(s) 258, 1493
BsnI GGCC 2 cut(s) 839, 923
Bsp119I TTCGAA 1 cut(s) 1409
Bsp1286I GDGCHC 3 cut(s) 486, 1205, 1459
Bsp1407I TGTACA 1 cut(s) 1227
Bsp143I GATC 3 cut(s) 366, 631, 1058
BspACI CCGC 4 cut(s) 19, 696, 920, 1004
BspANI GGCC 2 cut(s) 839, 923
BspCNI CTCAG 3 cut(s) 754, 856, 1496
BspLI GGNNCC 5 cut(s) 193, 246, 1060, 1194, 1544
BspPI GGATC 3 cut(s) 639, 1053, 1066
BspT104I TTCGAA 1 cut(s) 1409
BspT107I GGYRCC 1 cut(s) 1542
BsrBI CCGCTC 1 cut(s) 19
BsrDI GCAATG 1 cut(s) 310
BsrFI RCCGGY 1 cut(s) 194
BsrGI TGTACA 1 cut(s) 1227
BsrI ACTGG 1 cut(s) 33
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 4 cut(s) 176, 1062, 1072, 1238
BssMI GATC 3 cut(s) 366, 631, 1058
BssNI GRCGYC 1 cut(s) 67
BssSI CACGAG 1 cut(s) 1090
BssT1I CCWWGG 3 cut(s) 1062, 1072, 1238
Bst2BI CACGAG 1 cut(s) 1090
Bst2UI CCWGG 2 cut(s) 177, 1372
Bst4CI ACNGT 6 cut(s) 560, 692, 868, 1126, 1135, 1495
Bst6I CTCTTC 1 cut(s) 20
BstACI GRCGYC 1 cut(s) 67
BstAPI GCANNNNNTGC 1 cut(s) 116
BstAUI TGTACA 1 cut(s) 1227
BstBI TTCGAA 1 cut(s) 1409
BstC8I GCNNGC 1 cut(s) 1223
BstDEI CTNAG 5 cut(s) 576, 762, 843, 927, 1483
BstENI CCTNNNNNAGG 1 cut(s) 268
BstF5I GGATG 5 cut(s) 84, 277, 301, 961, 1471
BstKTI GATC 3 cut(s) 369, 634, 1061
BstMAI GTCTC 2 cut(s) 936, 1149
BstMBI GATC 3 cut(s) 366, 631, 1058
BstMCI CGRYCG 1 cut(s) 715
BstMWI GCNNNNNNNGC 4 cut(s) 107, 116, 200, 1261
BstNI CCWGG 2 cut(s) 177, 1372
BstNSI RCATGY 1 cut(s) 49
BstSCI CCNGG 4 cut(s) 175, 709, 1287, 1370
BstSLI GKGCMC 2 cut(s) 1205, 1459
BstV1I GCAGC 5 cut(s) 103, 190, 236, 892, 1248
BstV2I GAAGAC 1 cut(s) 62
BstX2I RGATCY 1 cut(s) 1058
BstXI CCANNNNNNTGG 1 cut(s) 255
BstYI RGATCY 1 cut(s) 1058
BsuI GTATCC 5 cut(s) 253, 523, 680, 993, 1436
BsuRI GGCC 2 cut(s) 839, 923
BtsCI GGATG 5 cut(s) 84, 277, 301, 961, 1471
BtsI GCAGTG 1 cut(s) 741
BtsIMutI CAGTG 6 cut(s) 697, 741, 759, 1122, 1205, 1491
Cac8I GCNNGC 1 cut(s) 1223
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 1 cut(s) 245
Csp6I GTAC 8 cut(s) 54, 92, 423, 556, 699, 1228, 1368, 1543
CviAII CATG 6 cut(s) 46, 236, 399, 800, 1339, 1452
CviQI GTAC 8 cut(s) 54, 92, 423, 556, 699, 1228, 1368, 1543
DdeI CTNAG 5 cut(s) 576, 762, 843, 927, 1483
DpnI GATC 3 cut(s) 368, 633, 1060
DpnII GATC 3 cut(s) 366, 631, 1058
DraIII CACNNNGTG 1 cut(s) 811
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 484
Eco130I CCWWGG 3 cut(s) 1062, 1072, 1238
Eco147I AGGCCT 1 cut(s) 839
Eco24I GRGCYC 1 cut(s) 486
Eco47I GGWCC 1 cut(s) 245
Eco53kI GAGCTC 1 cut(s) 484
Eco57I CTGAAG 2 cut(s) 237, 367
EcoICRI GAGCTC 1 cut(s) 484
EcoNI CCTNNNNNAGG 1 cut(s) 268
EcoRI GAATTC 1 cut(s) 1245
EcoRII CCWGG 2 cut(s) 175, 1370
EcoT14I CCWWGG 3 cut(s) 1062, 1072, 1238
EcoT22I ATGCAT 1 cut(s) 13
EcoT38I GRGCYC 1 cut(s) 486
ErhI CCWWGG 3 cut(s) 1062, 1072, 1238
FaeI CATG 6 cut(s) 49, 239, 402, 803, 1342, 1455
FalI AAGNNNNNCTT 4 cut(s) 356, 388, 1430, 1462
FaqI GGGAC 2 cut(s) 258, 1493
FatI CATG 6 cut(s) 45, 235, 398, 799, 1338, 1451
Fnu4HI GCNGC 7 cut(s) 20, 117, 204, 225, 906, 921, 1262
FokI GGATG 5 cut(s) 71, 264, 308, 948, 1478
FriOI GRGCYC 1 cut(s) 486
Fsp4HI GCNGC 7 cut(s) 20, 117, 204, 225, 906, 921, 1262
FspBI CTAG 2 cut(s) 165, 270
GluI GCNGC 7 cut(s) 20, 117, 204, 225, 906, 921, 1262
HaeIII GGCC 2 cut(s) 839, 923
HapII CCGG 3 cut(s) 195, 711, 1289
Hin1I GRCGYC 1 cut(s) 67
Hin1II CATG 6 cut(s) 49, 239, 402, 803, 1342, 1455
HincII GTYRAC 2 cut(s) 457, 1138
HindII GTYRAC 2 cut(s) 457, 1138
HindIII AAGCTT 1 cut(s) 1046
HinfI GANTC 5 cut(s) 136, 377, 453, 1040, 1481
HpaI GTTAAC 1 cut(s) 1138
HpaII CCGG 3 cut(s) 195, 711, 1289
HphI GGTGA 3 cut(s) 661, 989, 1447
Hpy166II GTNNAC 5 cut(s) 442, 457, 957, 1138, 1509
Hpy188I TCNGA 7 cut(s) 217, 295, 386, 474, 734, 846, 898
Hpy188III TCNNGA 7 cut(s) 281, 405, 596, 935, 1250, 1348, 1463
Hpy8I GTNNAC 5 cut(s) 442, 457, 957, 1138, 1509
HpyAV CCTTC 5 cut(s) 212, 274, 391, 443, 1432
HpyCH4III ACNGT 6 cut(s) 560, 692, 868, 1126, 1135, 1495
HpyCH4IV ACGT 1 cut(s) 67
HpyCH4V TGCA 9 cut(s) 11, 707, 741, 803, 908, 1103, 1264, 1451, 1559
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 116, 200, 1261
HpyF3I CTNAG 5 cut(s) 576, 762, 843, 927, 1483
HpySE526I ACGT 1 cut(s) 67
Hsp92I GRCGYC 1 cut(s) 67
Hsp92II CATG 6 cut(s) 49, 239, 402, 803, 1342, 1455
KpnI GGTACC 1 cut(s) 1546
KspAI GTTAAC 1 cut(s) 1138
Kzo9I GATC 3 cut(s) 366, 631, 1058
LmnI GCTCC 3 cut(s) 191, 221, 1340
Lsp1109I GCAGC 5 cut(s) 103, 190, 236, 892, 1248
LweI GCATC 3 cut(s) 138, 970, 1096
MaeI CTAG 2 cut(s) 165, 270
MaeII ACGT 1 cut(s) 67
MaeIII GTNAC 7 cut(s) 511, 649, 667, 749, 871, 967, 1126
MalI GATC 3 cut(s) 368, 633, 1060
MbiI CCGCTC 1 cut(s) 19
MboI GATC 3 cut(s) 366, 631, 1058
MboII GAAGA 5 cut(s) 37, 62, 560, 826, 1388
MflI RGATCY 1 cut(s) 1058
MhlI GDGCHC 3 cut(s) 486, 1205, 1459
MlyI GAGTC 4 cut(s) 145, 371, 462, 1475
MmeI TCCRAC 1 cut(s) 712
Mph1103I ATGCAT 1 cut(s) 13
MseI TTAA 8 cut(s) 156, 429, 569, 639, 1053, 1137, 1431, 1569
MspA1I CMGCKG 1 cut(s) 905
MspI CCGG 3 cut(s) 195, 711, 1289
MspR9I CCNGG 4 cut(s) 177, 711, 1289, 1372
MvaI CCWGG 2 cut(s) 177, 1372
MwoI GCNNNNNNNGC 4 cut(s) 107, 116, 200, 1261
NciI CCSGG 2 cut(s) 711, 1289
NdeII GATC 3 cut(s) 366, 631, 1058
NlaIII CATG 6 cut(s) 49, 239, 402, 803, 1342, 1455
NlaIV GGNNCC 5 cut(s) 193, 246, 1060, 1194, 1544
NmuCI GTSAC 3 cut(s) 511, 649, 871
NsiI ATGCAT 1 cut(s) 13
NspI RCATGY 1 cut(s) 49
NspV TTCGAA 1 cut(s) 1409
PceI AGGCCT 1 cut(s) 839
PciI ACATGT 1 cut(s) 45
PfeI GAWTC 1 cut(s) 1040
PkrI GCNGC 7 cut(s) 21, 118, 205, 226, 907, 922, 1263
PleI GAGTC 4 cut(s) 144, 371, 461, 1475
PpsI GAGTC 4 cut(s) 144, 371, 461, 1475
PscI ACATGT 1 cut(s) 45
Psp124BI GAGCTC 1 cut(s) 486
Psp6I CCWGG 2 cut(s) 175, 1370
PspGI CCWGG 2 cut(s) 175, 1370
PspN4I GGNNCC 5 cut(s) 193, 246, 1060, 1194, 1544
PspPI GGNCC 1 cut(s) 245
PsuI RGATCY 1 cut(s) 1058
PvuII CAGCTG 1 cut(s) 905
RsaI GTAC 8 cut(s) 55, 93, 424, 557, 700, 1229, 1369, 1544
RsaNI GTAC 8 cut(s) 54, 92, 423, 556, 699, 1228, 1368, 1543
SacI GAGCTC 1 cut(s) 486
SaqAI TTAA 8 cut(s) 156, 429, 569, 639, 1053, 1137, 1431, 1569
SatI GCNGC 7 cut(s) 20, 117, 204, 225, 906, 921, 1262
Sau3AI GATC 3 cut(s) 366, 631, 1058
Sau96I GGNCC 1 cut(s) 245
SchI GAGTC 4 cut(s) 145, 371, 462, 1475
ScrFI CCNGG 4 cut(s) 177, 711, 1289, 1372
SduI GDGCHC 3 cut(s) 486, 1205, 1459
SfaNI GCATC 3 cut(s) 138, 970, 1096
SfuI TTCGAA 1 cut(s) 1409
SinI GGWCC 1 cut(s) 245
SmlI CTYRAG 2 cut(s) 1358, 1547
SmoI CTYRAG 2 cut(s) 1358, 1547
SseBI AGGCCT 1 cut(s) 839
SsiI CCGC 4 cut(s) 19, 696, 920, 1004
SspMI CTAG 2 cut(s) 165, 270
SstI GAGCTC 1 cut(s) 486
StuI AGGCCT 1 cut(s) 839
StyD4I CCNGG 4 cut(s) 175, 709, 1287, 1370
StyI CCWWGG 3 cut(s) 1062, 1072, 1238
TaaI ACNGT 6 cut(s) 560, 692, 868, 1126, 1135, 1495
TaiI ACGT 1 cut(s) 70
TaqI TCGA 6 cut(s) 146, 160, 715, 1249, 1409, 1498
TatI WGTACW 1 cut(s) 1227
TauI GCSGC 2 cut(s) 22, 923
TfiI GAWTC 1 cut(s) 1040
Tru1I TTAA 8 cut(s) 156, 429, 569, 639, 1053, 1137, 1431, 1569
Tru9I TTAA 8 cut(s) 156, 429, 569, 639, 1053, 1137, 1431, 1569
TscAI CASTG 6 cut(s) 697, 748, 766, 1129, 1212, 1498
TseFI GTSAC 3 cut(s) 511, 649, 871
TseI GCWGC 5 cut(s) 116, 203, 224, 905, 1261
Tsp45I GTSAC 3 cut(s) 511, 649, 871
TspDTI ATGAA 5 cut(s) 73, 172, 224, 1488, 1550
TspGWI ACGGA 1 cut(s) 1193
TspRI CASTG 6 cut(s) 697, 748, 766, 1129, 1212, 1498
VpaK11BI GGWCC 1 cut(s) 245
XagI CCTNNNNNAGG 1 cut(s) 268
XapI RAATTY 3 cut(s) 288, 1025, 1245
XceI RCATGY 1 cut(s) 49
XcmI CCANNNNNNNNNTGG 1 cut(s) 1148
XspI CTAG 2 cut(s) 165, 270
ZraI GACGTC 1 cut(s) 68
Zsp2I ATGCAT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.