Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0003709

Physical Location & Seq

Genomic Coordinates Forward (+)

848886 .. 853565

Gene Structure 4680 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g00250.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1620 bp

TGGAGCTCGAGGTCTGAACTGAACTCTATAAATAGGCTTGATTGTATCAGTTACGTGAAAACGCAGCAGTATTCAACATTATATATGGCGTTTGGAGGTTCTGTGTTCTTTTCGTTGCTGCTAGCTTTGGCTGCGGCTGCTAGCTTCTCCTTTGGCACTGCCCGTGCTTACTATATCCCTTTTAACCGAAGCAGTTTTCCGAGTGATTTCCTTTTTGGTGTTGGTTCAGCTGCTTATCAGTCAGAAGGAGCAGCATTAGTCGACGGAAGGGGACCTAGCATCTGGGATACTTTTGTTAGAAAGCATCCAGAAAAGATATGGGATCACAGCACCGGAGATGTAGCAAATGACTTTTACCATCGTTATAAGGAAGATATCAAATTGATGAAGAAAATTGGGTTGGACACCTTCAGATTCTCAATCTCATGGTCCAGATTACTTCCAAAGGGAAAACTCAGCGGAGGAGTCAACCCACTCGGTGTCAAATTTTACAACAATCTCATCAATGAGCTCGTATCAAACGGTATCAAGCCCTTTGTGACTTTACTCCATTATGATCCTCCCCAAGCACTTGATGAACTGTATGGCGGGCTCTTAAGCCGAAACATAATAAACGATTACGGAGATTATGTGGACTTTTGTTTCAAGACTTTTGGTGATCGAGTAAAGCACTGGGTCACGATGAACGAACCTAACGGATTTGCTATTAATGGCTACAACGGCGGTACCTTTGCGCCGGGGCGGTGTTCTAACTATGAGGGTAACTGCACTTCCGGTAACTCAGCGACTGAACCCTACATTGCGGCACACCATTTGCTTCTTGCTCATTCAGCTGCTGTCAAGATATACAAGGACAAATATCAAGCTACCCAAAAGGGACAGATCGGGATTACCATAGTGACTCACTGGTGGAAACCTAAGTTCCCAGAACAGCTGGCTAGCCGAAGAGCCACTCTTAGATCACTCGACTTTATGTTTGGATGGTTTGCCAATCCTATATTTCATGGGGATTATCCGGCGGTCATGAAATCTTTGGTCGGGGATCGATTGCCGAAATTTACAGAAGCTGAGTCGAAGCAGATAAAAGGCTCCCTCGATTTTCTTGGGTTGAATTACTACACAGCATATTACACAGTTGATGAACCAGCTTTATCCAACGCTGTCAACCATAGTTGGAGTGCAGACAGACAAGTCGGTGCTTCTACTACTGATAAAAATGGGGCTCTTATTGGTGCAGCGACTGATCTGGATTGGCTTTTCGTTTATCCGAAAGGGATCAGAGAGGTTCTTTTGTATATAAAGGAGAAGTACAATAACCCAAACATTTATATTACTGAGAATGGATATGCATACGGATATAATGCCTCAACACCAATTCAAGAAGCTCGTAAGGATAATTTGAGGATAAGATACCATCACGACCATTTGTGGTATCTTTTGAAGGCTATTAAGGATGGGGTAAAAGTGAAGGGATACTACGCATGGTCATTTTGGGATGACTTCGAATGGGATGCTGGTTACACAGTTGGGTTTGGCCTAACATTAGTTGATGTCAAGGATAATCTGAAAAGATACCTCAAGTATTCTGCCTATTGGTACAAGATGTTCCTCCTGCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0008150 GO:0008152 GO:0008422 GO:0015926 GO:0016787 GO:0016798 GO:0071704 GO:1901135 GO:1901657

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_1

Protein Analysis

540

Amino Acids

61.28

Weight (kDa)

8.8

Isoelectric Point (pI)

23.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_1	PF00232	64 - 535	7.8e-151	Glycosyl hydrolase family 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003709)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G44480 AT2G44480 AT2G44480 AT2G44480 AT2G44480
fragaria_vesca	FvH4_7g00214 FvH4_7g00214 FvH4_7g00214 FvH4_7g00214 FvH4_7g00250 FvH4_7g00270
malus_domestica	MD07G1003400.v1.1
prunus_persica	Prupe.2G005100_v2.0.a1
pyrus_communis	pycom07g00340
rosa_chinensis	RchiOBHm_Chr1g0313661 RchiOBHm_Chr1g0313711 RchiOBHm_Chr1g0313741
rosa_laevigata	RLG00000030780 RLG00000030783 RLG00000030785
rosa_multiflora	Rmu_sc0009740.1_g000006 Rmu_ssc0000027.1_g000024
rosa_roxburghii	Rroxscaffold_4G00332110 Rroxscaffold_4G00332130
rosa_rugosa	RorugPtG0005800 RorugPtG0005900.1 RorugPtG0006000.1
rosa_samantha	Rh1AG005800 Rh1CG008900 Rh1CG009000 Rh1DG004000 Rh1DG004200
rosa_wichuraiana	Rw1G000340 Rw1G000350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	366
AasI	GACNNNNNNGTC	1 cut(s)	1190
Acc65I	GGTACC	1 cut(s)	725
AccB1I	GGYRCC	1 cut(s)	725
AccI	GTMKAC	1 cut(s)	261
AciI	CCGC	7 cut(s)	134, 459, 588, 723, 742, 803, 1019
AclWI	GGATC	4 cut(s)	330, 551, 1050, 1283
AcsI	RAATTY	2 cut(s)	485, 1055
AcuI	CTGAAG	1 cut(s)	394
AdeI	CACNNNGTG	1 cut(s)	479
AfaI	GTAC	3 cut(s)	727, 1310, 1598
AflII	CTTAAG	1 cut(s)	595
AgsI	TTSAA	5 cut(s)	75, 646, 1111, 1379, 1441
AjuI	GAANNNNNNNTTGG	2 cut(s)	383, 415
Alw21I	GWGCWC	2 cut(s)	8, 513
AlwI	GGATC	4 cut(s)	330, 551, 1050, 1283
AlwNI	CAGNNNCTG	4 cut(s)	788, 836, 1067, 1241
Ama87I	CYCGRG	1 cut(s)	7
AoxI	GGCC	1 cut(s)	1534
ApeKI	GCWGC	8 cut(s)	64, 118, 131, 137, 230, 251, 833, 1235
ApoI	RAATTY	2 cut(s)	485, 1055
ArsI	GACNNNNNNTTYG	2 cut(s)	959, 991
AseI	ATTAAT	1 cut(s)	708
Asp718I	GGTACC	1 cut(s)	725
AspLEI	GCGC	1 cut(s)	736
AspS9I	GGNCC	2 cut(s)	272, 429
AsuC2I	CCSGG	1 cut(s)	738
AsuHPI	GGTGA	1 cut(s)	668
AsuII	TTCGAA	1 cut(s)	1503
AsuNHI	GCTAGC	3 cut(s)	121, 140, 938
AvaI	CYCGRG	1 cut(s)	7
AvaII	GGWCC	2 cut(s)	272, 429
BaeI	ACNNNNGTAYC	4 cut(s)	279, 312, 709, 742
BanI	GGYRCC	1 cut(s)	725
BanII	GRGCYC	4 cut(s)	8, 513, 594, 1225
BarI	GAAGNNNNNNTAC	4 cut(s)	754, 786, 1460, 1492
Bbv12I	GWGCWC	2 cut(s)	8, 513
BbvI	GCAGC	8 cut(s)	76, 105, 118, 124, 217, 263, 820, 1247
BccI	CCATC	4 cut(s)	366, 975, 1422, 1448
BceAI	ACGGC	1 cut(s)	736
BcgI	CGANNNNNNTGC	2 cut(s)	1493, 1527
BciVI	GTATCC	2 cut(s)	280, 1466
BcnI	CCSGG	1 cut(s)	738
BfaI	CTAG	4 cut(s)	122, 141, 276, 939
BfrI	CTTAAG	1 cut(s)	595
BfuI	GTATCC	2 cut(s)	280, 1466
Bme1390I	CCNGG	1 cut(s)	738
Bme18I	GGWCC	2 cut(s)	272, 429
BmeT110I	CYCGRG	1 cut(s)	7
BmgT120I	GGNCC	2 cut(s)	272, 429
BmiI	GGNNCC	3 cut(s)	273, 727, 1090
BmrFI	CCNGG	1 cut(s)	738
BmrI	ACTGGG	1 cut(s)	682
BmsI	GCATC	3 cut(s)	288, 313, 1501
BmtI	GCTAGC	3 cut(s)	125, 144, 942
BmuI	ACTGGG	1 cut(s)	682
Bpu14I	TTCGAA	1 cut(s)	1503
BpuEI	CTTGAG	1 cut(s)	1562
BpuMI	CCSGG	1 cut(s)	738
Bsa29I	ATCGAT	1 cut(s)	1045
BsaAI	YACGTR	1 cut(s)	55
BsaJI	CCNNGG	1 cut(s)	737
BsaWI	WCCGGW	2 cut(s)	332, 773
BsaXI	ACNNNNNCTCC	4 cut(s)	87, 117, 615, 645
Bse1I	ACTGG	2 cut(s)	677, 911
Bse3DI	GCAATG	1 cut(s)	798
BseCI	ATCGAT	1 cut(s)	1045
BseDI	CCNNGG	1 cut(s)	737
BseGI	GGATG	5 cut(s)	304, 986, 1459, 1501, 1516
BseMI	GCAATG	1 cut(s)	798
BseMII	CTCAG	4 cut(s)	469, 795, 1059, 1326
BseNI	ACTGG	2 cut(s)	677, 911
BseRI	GAGGAG	1 cut(s)	477
BseXI	GCAGC	8 cut(s)	76, 105, 118, 124, 217, 263, 820, 1247
BsgI	GTGCAG	4 cut(s)	751, 1200, 1254, 1598
BshFI	GGCC	1 cut(s)	1536
BshNI	GGYRCC	1 cut(s)	725
BshVI	ATCGAT	1 cut(s)	1045
BsiHKAI	GWGCWC	2 cut(s)	8, 513
BsiHKCI	CYCGRG	1 cut(s)	7
BsiSI	CCGG	4 cut(s)	333, 737, 774, 1016
BslFI	GGGAC	2 cut(s)	285, 891
BsmFI	GGGAC	2 cut(s)	285, 891
BsnI	GGCC	1 cut(s)	1536
BsoBI	CYCGRG	1 cut(s)	7
Bsp119I	TTCGAA	1 cut(s)	1503
Bsp1286I	GDGCHC	4 cut(s)	8, 513, 594, 1225
Bsp143I	GATC	8 cut(s)	322, 556, 658, 882, 959, 1042, 1243, 1275
BspACI	CCGC	7 cut(s)	134, 459, 588, 723, 742, 803, 1019
BspANI	GGCC	1 cut(s)	1536
BspCNI	CTCAG	4 cut(s)	468, 794, 1060, 1327
BspDI	ATCGAT	1 cut(s)	1045
BspHI	TCATGA	1 cut(s)	1023
BspLI	GGNNCC	3 cut(s)	273, 727, 1090
BspOI	GCTAGC	3 cut(s)	125, 144, 942
BspPI	GGATC	4 cut(s)	330, 551, 1050, 1283
BspQI	GCTCTTC	1 cut(s)	940
BspT104I	TTCGAA	1 cut(s)	1503
BspT107I	GGYRCC	1 cut(s)	725
BspTI	CTTAAG	1 cut(s)	595
BsrDI	GCAATG	1 cut(s)	798
BsrI	ACTGG	2 cut(s)	677, 911
BssECI	CCNNGG	1 cut(s)	737
BssMI	GATC	8 cut(s)	322, 556, 658, 882, 959, 1042, 1243, 1275
Bst4CI	ACNGT	4 cut(s)	524, 582, 1135, 1525
Bst6I	CTCTTC	1 cut(s)	940
BstAFI	CTTAAG	1 cut(s)	595
BstBAI	YACGTR	1 cut(s)	55
BstBI	TTCGAA	1 cut(s)	1503
BstC8I	GCNNGC	5 cut(s)	123, 142, 590, 936, 940
BstDEI	CTNAG	6 cut(s)	455, 781, 918, 956, 1068, 1335
BstF5I	GGATG	5 cut(s)	304, 986, 1459, 1501, 1516
BstHHI	GCGC	1 cut(s)	736
BstKTI	GATC	8 cut(s)	325, 559, 661, 885, 962, 1045, 1246, 1278
BstMBI	GATC	8 cut(s)	322, 556, 658, 882, 959, 1042, 1243, 1275
BstMWI	GCNNNNNNNGC	4 cut(s)	131, 137, 720, 830
BstSCI	CCNGG	1 cut(s)	736
BstV1I	GCAGC	8 cut(s)	76, 105, 118, 124, 217, 263, 820, 1247
Bsu15I	ATCGAT	1 cut(s)	1045
BsuI	GTATCC	2 cut(s)	280, 1466
BsuRI	GGCC	1 cut(s)	1536
BsuTUI	ATCGAT	1 cut(s)	1045
BtsCI	GGATG	5 cut(s)	304, 986, 1459, 1501, 1516
BtsI	GCAGTG	1 cut(s)	156
BtsIMutI	CAGTG	4 cut(s)	156, 670, 904, 1615
Cac8I	GCNNGC	5 cut(s)	123, 142, 590, 936, 940
CaiI	CAGNNNCTG	4 cut(s)	788, 836, 1067, 1241
CciI	TCATGA	1 cut(s)	1023
CfoI	GCGC	1 cut(s)	736
Cfr13I	GGNCC	2 cut(s)	272, 429
ClaI	ATCGAT	1 cut(s)	1045
Csp6I	GTAC	3 cut(s)	726, 1309, 1597
CviAII	CATG	4 cut(s)	426, 1004, 1024, 1482
CviQI	GTAC	3 cut(s)	726, 1309, 1597
DdeI	CTNAG	6 cut(s)	455, 781, 918, 956, 1068, 1335
DpnI	GATC	8 cut(s)	324, 558, 660, 884, 961, 1044, 1245, 1277
DpnII	GATC	8 cut(s)	322, 556, 658, 882, 959, 1042, 1243, 1275
DraIII	CACNNNGTG	1 cut(s)	479
DrdI	GACNNNNNNGTC	1 cut(s)	1190
DseDI	GACNNNNNNGTC	1 cut(s)	1190
Eam1104I	CTCTTC	1 cut(s)	940
EarI	CTCTTC	1 cut(s)	940
Ecl136II	GAGCTC	2 cut(s)	6, 511
Eco24I	GRGCYC	4 cut(s)	8, 513, 594, 1225
Eco32I	GATATC	1 cut(s)	376
Eco47I	GGWCC	2 cut(s)	272, 429
Eco53kI	GAGCTC	2 cut(s)	6, 511
Eco57I	CTGAAG	1 cut(s)	394
Eco88I	CYCGRG	1 cut(s)	7
EcoICRI	GAGCTC	2 cut(s)	6, 511
EcoO109I	RGGNCCY	1 cut(s)	272
EcoRV	GATATC	1 cut(s)	376
EcoT22I	ATGCAT	1 cut(s)	1351
EcoT38I	GRGCYC	4 cut(s)	8, 513, 594, 1225
FaeI	CATG	4 cut(s)	429, 1007, 1027, 1485
FaqI	GGGAC	2 cut(s)	285, 891
FatI	CATG	4 cut(s)	425, 1003, 1023, 1481
FauI	CCCGC	1 cut(s)	581
FblI	GTMKAC	1 cut(s)	261
FokI	GGATG	5 cut(s)	291, 993, 1466, 1508, 1523
FriOI	GRGCYC	4 cut(s)	8, 513, 594, 1225
FspBI	CTAG	4 cut(s)	122, 141, 276, 939
GlaI	GCGC	1 cut(s)	735
HaeIII	GGCC	1 cut(s)	1536
HapII	CCGG	4 cut(s)	333, 737, 774, 1016
HhaI	GCGC	1 cut(s)	736
Hin1II	CATG	4 cut(s)	429, 1007, 1027, 1485
Hin6I	GCGC	1 cut(s)	734
HinP1I	GCGC	1 cut(s)	734
HincII	GTYRAC	3 cut(s)	262, 469, 1165
HindII	GTYRAC	3 cut(s)	262, 469, 1165
HinfI	GANTC	4 cut(s)	414, 465, 901, 1070
HpaII	CCGG	4 cut(s)	333, 737, 774, 1016
HphI	GGTGA	1 cut(s)	668
Hpy166II	GTNNAC	4 cut(s)	262, 469, 634, 1165
Hpy188I	TCNGA	7 cut(s)	16, 201, 244, 413, 1269, 1280, 1566
Hpy8I	GTNNAC	4 cut(s)	262, 469, 634, 1165
Hpy99I	CGWCG	1 cut(s)	266
HpyAV	CCTTC	5 cut(s)	239, 261, 418, 1435, 1462
HpyCH4III	ACNGT	4 cut(s)	524, 582, 1135, 1525
HpyCH4IV	ACGT	1 cut(s)	54
HpyCH4V	TGCA	5 cut(s)	768, 1181, 1235, 1349, 1615
HpyF10VI	GCNNNNNNNGC	4 cut(s)	131, 137, 720, 830
HpyF3I	CTNAG	6 cut(s)	455, 781, 918, 956, 1068, 1335
HpySE526I	ACGT	1 cut(s)	54
Hsp92II	CATG	4 cut(s)	429, 1007, 1027, 1485
HspAI	GCGC	1 cut(s)	734
KpnI	GGTACC	1 cut(s)	729
Kzo9I	GATC	8 cut(s)	322, 556, 658, 882, 959, 1042, 1243, 1275
LguI	GCTCTTC	1 cut(s)	940
LmnI	GCTCC	2 cut(s)	248, 1094
Lsp1109I	GCAGC	8 cut(s)	76, 105, 118, 124, 217, 263, 820, 1247
LweI	GCATC	3 cut(s)	288, 313, 1501
MaeI	CTAG	4 cut(s)	122, 141, 276, 939
MaeII	ACGT	1 cut(s)	54
MaeIII	GTNAC	7 cut(s)	50, 538, 676, 761, 776, 898, 1517
MalI	GATC	8 cut(s)	324, 558, 660, 884, 961, 1044, 1245, 1277
MboI	GATC	8 cut(s)	322, 556, 658, 882, 959, 1042, 1243, 1275
MboII	GAAGA	3 cut(s)	383, 400, 957
MhlI	GDGCHC	4 cut(s)	8, 513, 594, 1225
MluCI	AATT	7 cut(s)	380, 393, 485, 1055, 1111, 1374, 1396
MlyI	GAGTC	3 cut(s)	474, 895, 1079
MmeI	TCCRAC	3 cut(s)	381, 1154, 1179
Mph1103I	ATGCAT	1 cut(s)	1351
MseI	TTAA	4 cut(s)	183, 596, 708, 1449
MspA1I	CMGCKG	4 cut(s)	230, 459, 833, 934
MspCI	CTTAAG	1 cut(s)	595
MspI	CCGG	4 cut(s)	333, 737, 774, 1016
MspR9I	CCNGG	1 cut(s)	738
MwoI	GCNNNNNNNGC	4 cut(s)	131, 137, 720, 830
NciI	CCSGG	1 cut(s)	738
NdeII	GATC	8 cut(s)	322, 556, 658, 882, 959, 1042, 1243, 1275
NheI	GCTAGC	3 cut(s)	121, 140, 938
NlaIII	CATG	4 cut(s)	429, 1007, 1027, 1485
NlaIV	GGNNCC	3 cut(s)	273, 727, 1090
NmuCI	GTSAC	3 cut(s)	538, 676, 898
NsiI	ATGCAT	1 cut(s)	1351
NspV	TTCGAA	1 cut(s)	1503
PaeR7I	CTCGAG	1 cut(s)	7
PagI	TCATGA	1 cut(s)	1023
PciSI	GCTCTTC	1 cut(s)	940
PfeI	GAWTC	1 cut(s)	414
PleI	GAGTC	3 cut(s)	473, 895, 1078
PpsI	GAGTC	3 cut(s)	473, 895, 1078
Ppu21I	YACGTR	1 cut(s)	55
PpuMI	RGGWCCY	1 cut(s)	272
PshBI	ATTAAT	1 cut(s)	708
PsiI	TTATAA	1 cut(s)	366
Psp124BI	GAGCTC	2 cut(s)	8, 513
Psp5II	RGGWCCY	1 cut(s)	272
PspN4I	GGNNCC	3 cut(s)	273, 727, 1090
PspPI	GGNCC	2 cut(s)	272, 429
PspPPI	RGGWCCY	1 cut(s)	272
PspXI	VCTCGAGB	1 cut(s)	7
PsrI	GAACNNNNNNTAC	1 cut(s)	1589
PstNI	CAGNNNCTG	4 cut(s)	788, 836, 1067, 1241
PvuII	CAGCTG	3 cut(s)	230, 833, 934
RsaI	GTAC	3 cut(s)	727, 1310, 1598
RsaNI	GTAC	3 cut(s)	726, 1309, 1597
SacI	GAGCTC	2 cut(s)	8, 513
SalI	GTCGAC	1 cut(s)	260
SapI	GCTCTTC	1 cut(s)	940
SaqAI	TTAA	4 cut(s)	183, 596, 708, 1449
Sau3AI	GATC	8 cut(s)	322, 556, 658, 882, 959, 1042, 1243, 1275
Sau96I	GGNCC	2 cut(s)	272, 429
SchI	GAGTC	3 cut(s)	474, 895, 1079
ScrFI	CCNGG	1 cut(s)	738
SduI	GDGCHC	4 cut(s)	8, 513, 594, 1225
SfaNI	GCATC	3 cut(s)	288, 313, 1501
Sfr274I	CTCGAG	1 cut(s)	7
SfuI	TTCGAA	1 cut(s)	1503
SinI	GGWCC	2 cut(s)	272, 429
SlaI	CTCGAG	1 cut(s)	7
SmlI	CTYRAG	3 cut(s)	7, 595, 1577
SmoI	CTYRAG	3 cut(s)	7, 595, 1577
Sse9I	AATT	7 cut(s)	380, 393, 485, 1055, 1111, 1374, 1396
SsiI	CCGC	7 cut(s)	134, 459, 588, 723, 742, 803, 1019
SspMI	CTAG	4 cut(s)	122, 141, 276, 939
SstI	GAGCTC	2 cut(s)	8, 513
StyD4I	CCNGG	1 cut(s)	736
TaaI	ACNGT	4 cut(s)	524, 582, 1135, 1525
TaiI	ACGT	1 cut(s)	57
TaqI	TCGA	8 cut(s)	8, 261, 661, 966, 1045, 1073, 1095, 1503
TasI	AATT	7 cut(s)	380, 393, 485, 1055, 1111, 1374, 1396
TatI	WGTACW	1 cut(s)	1308
TauI	GCSGC	2 cut(s)	137, 806
TfiI	GAWTC	1 cut(s)	414
Tru1I	TTAA	4 cut(s)	183, 596, 708, 1449
Tru9I	TTAA	4 cut(s)	183, 596, 708, 1449
TscAI	CASTG	3 cut(s)	163, 677, 911
TseFI	GTSAC	3 cut(s)	538, 676, 898
TseI	GCWGC	8 cut(s)	64, 118, 131, 137, 230, 251, 833, 1235
Tsp45I	GTSAC	3 cut(s)	538, 676, 898
TspDTI	ATGAA	6 cut(s)	401, 591, 698, 992, 1040, 1155
TspGWI	ACGGA	4 cut(s)	279, 636, 711, 1368
TspRI	CASTG	3 cut(s)	163, 677, 911
Vha464I	CTTAAG	1 cut(s)	595
VpaK11BI	GGWCC	2 cut(s)	272, 429
VspI	ATTAAT	1 cut(s)	708
XapI	RAATTY	2 cut(s)	485, 1055
XcmI	CCANNNNNNNNNTGG	1 cut(s)	315
XhoI	CTCGAG	1 cut(s)	7
XmiI	GTMKAC	1 cut(s)	261
XspI	CTAG	4 cut(s)	122, 141, 276, 939
Zsp2I	ATGCAT	1 cut(s)	1351

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.