Rh1CG009000

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0003709
Physical Location & Seq
Forward (+)
1335214 .. 1345482
10269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG009000.1

Sequence Viewer

Length: 1536 bp
ATGGCATTTGGAGGTTCTGTGTTCTTGTCTGTGCTCCTAGCTTTAGCTGCAGCTGCCAGCGTCTCCGTCAGCACTTCCCATGCTTATTATATCCCATTTAACCGAAGCAGTTTTCCAAATGGATTCCTTTTTGGTGCTGGTTCAGCTGCTTATCAGTCAGAAGGAGCAGCATTTCTACATGGAAGGGGACCTAGCATCTGGGATACTTTCGTTAGGAAAAATCCAGAAAAGATATCAGATCACAGCACCGGAGACGTAGCTGATGACTTTTATCACCACTATAAGGAGGATATAAAATTGATGAAGAAAATTGGGTTGGACACCTTCAGAATGTCAATTTCATGGACCAGATTACTTCCTAAGGGAAAACTTAGTGGAGGAGTGAACCCACTTGGTGTACAATTCTACAACAATCTCATCAATGAGCTCGTATCCAACGGTATCAAGCCCTTCGTGACTTTGCTCCATTATGATCCTCCCCAAGCACTTGAGGATCTGTATGGCGGGTTCTTAAGCCCTAACATAATAAATGATTACGGAGATTATGTGGATTTCTGCTTCAAGACATTTGGTGATCGGGTGAAGCACTGGGTCACAATGAACGAACCTAACGGATTTGCTATTAGTGGCTACGACGGCGGAACCTTTGCACCAGGTCGGTGTTCTAACTATGAGGGAAATTGCACTTCCGGTAATTCTGCGACTGAACCCTACATTGTGGCACACCATTTGCTTCTTGCGCATTCAGCCGCTGTCAAAATATACAAGGACAAATATCAAGCTTCCCAAAAGGGACAGATCGGGATCACAGTAGTGACTCACTGGTGGAAACCTAAGTTCCCAACACAGCTGGCAAGCCGGACGGCCACCCTTAGGTCACTTGATTTTATGTTCGGATGGTTTGCCAATCCTATAATTCATGGTGATTATCCAAAGGTCATGCGATCTTTGGTCGGGAATCGATTGCCGAAATTTACAGAAGCTGAATCGAAGCAGATAAAAGGCTCCCTTGATTTTCTTGGGTTGAATTACTACACATCATATTATACAGAAGATGCACCAGCTTCATCCAACGCTGTCAACCAGAGTTGGACCGCAGACAGACAAGTAGCTGCTTCTACTACTGATAAAAATGGGGCTCTTATTGGTGCAGCGACTGATTTGGATTGGCTTTTCGTTTATCCGAAGGGGATCAGAGAGGTTCTTGTGTATATAAAGGAAAAGTACAATAACCCTGACATTTACATAACCGAGAATGGCTATGCATATGGATATAATGCTTCAATGCCAATTCAAGAAGCTCGTAAGGATAATTTGAGAATAAGATACCATCACGACCATCTGTGGTATCTTTTGAAAGCTATTAAGGATGGGGTTAAAGTGAAGGGATACTATGCATGGTCATTTTTTGATGACTTCGAATGGGATGCCGGTTACACAGTTGGGTTTGGCCTAACATTAGTTGATTTCAAGGATAATCTGAGAAGATACCTCAAATATTCTGCCTATTGGTTCAAGATGTTCCTCCTGCACTGA

Protein Analysis

511

Amino Acids

57.63

Weight (kDa)

8.37

Isoelectric Point (pI)

25.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 36 - 507 2.4e-148 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 741
AciI CCGC 4 cut(s) 504, 639, 750, 1095
AclWI GGATC 4 cut(s) 467, 501, 812, 1199
AcoI YGGCCR 1 cut(s) 864
AcsI RAATTY 1 cut(s) 971
AcuI CTGAAG 1 cut(s) 310
AdeI CACNNNGTG 1 cut(s) 395
AfaI GTAC 2 cut(s) 399, 1226
AfiI CCNNNNNNNGG 2 cut(s) 283, 873
AflII CTTAAG 1 cut(s) 511
AgsI TTSAA 7 cut(s) 562, 1027, 1284, 1295, 1357, 1471, 1516
AjnI CCWGG 1 cut(s) 652
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
AleI CACNNNNGTG 1 cut(s) 812
Alw21I GWGCWC 2 cut(s) 36, 429
Alw26I GTCTC 2 cut(s) 67, 246
AlwI GGATC 4 cut(s) 467, 501, 812, 1199
AlwNI CAGNNNCTG 3 cut(s) 752, 983, 1157
AoxI GGCC 2 cut(s) 864, 1450
ApeKI GCWGC 7 cut(s) 47, 50, 53, 146, 167, 1112, 1151
ApoI RAATTY 1 cut(s) 971
AspLEI GCGC 1 cut(s) 742
AspS9I GGNCC 3 cut(s) 188, 345, 1092
AsuHPI GGTGA 4 cut(s) 266, 584, 592, 935
AsuII TTCGAA 1 cut(s) 1419
AvaII GGWCC 3 cut(s) 188, 345, 1092
AxyI CCTNAGG 2 cut(s) 360, 872
BaeI ACNNNNGTAYC 2 cut(s) 195, 228
BanII GRGCYC 2 cut(s) 429, 1141
Bbv12I GWGCWC 2 cut(s) 36, 429
BbvI GCAGC 7 cut(s) 34, 40, 62, 133, 179, 1099, 1163
BccI CCATC 4 cut(s) 891, 1338, 1347, 1364
BceAI ACGGC 2 cut(s) 652, 879
BcgI CGANNNNNNTGC 2 cut(s) 1409, 1443
BciT130I CCWGG 1 cut(s) 654
BciVI GTATCC 3 cut(s) 196, 442, 1382
BcoDI GTCTC 2 cut(s) 67, 246
BfaI CTAG 2 cut(s) 38, 192
BfmI CTRYAG 1 cut(s) 48
BfrI CTTAAG 1 cut(s) 511
BfuI GTATCC 3 cut(s) 196, 442, 1382
BisI GCNGC 8 cut(s) 48, 51, 54, 147, 168, 750, 1113, 1152
BlsI GCNGC 8 cut(s) 49, 52, 55, 148, 169, 751, 1114, 1153
Bme1390I CCNGG 1 cut(s) 654
Bme18I GGWCC 3 cut(s) 188, 345, 1092
BmgT120I GGNCC 3 cut(s) 188, 345, 1092
BmiI GGNNCC 3 cut(s) 189, 643, 1006
BmrFI CCNGG 1 cut(s) 654
BmrI ACTGGG 1 cut(s) 598
BmsI GCATC 3 cut(s) 204, 1045, 1417
BmuI ACTGGG 1 cut(s) 598
Bpu14I TTCGAA 1 cut(s) 1419
BpuEI CTTGAG 1 cut(s) 509
Bsa29I ATCGAT 1 cut(s) 961
BsaBI GATNNNNATC 1 cut(s) 803
BsaWI WCCGGW 2 cut(s) 248, 689
BsaXI ACNNNNNCTCC 3 cut(s) 33, 531, 561
Bsc4I CCNNNNNNNGG 2 cut(s) 283, 873
Bse118I RCCGGY 1 cut(s) 1430
Bse1I ACTGG 2 cut(s) 593, 827
Bse21I CCTNAGG 2 cut(s) 360, 872
Bse8I GATNNNNATC 1 cut(s) 803
BseBI CCWGG 1 cut(s) 654
BseCI ATCGAT 1 cut(s) 961
BseGI GGATG 4 cut(s) 902, 1067, 1375, 1432
BseJI GATNNNNATC 1 cut(s) 803
BseLI CCNNNNNNNGG 2 cut(s) 283, 873
BseMII CTCAG 1 cut(s) 1472
BseNI ACTGG 2 cut(s) 593, 827
BseRI GAGGAG 1 cut(s) 393
BseXI GCAGC 7 cut(s) 34, 40, 62, 133, 179, 1099, 1163
BsgI GTGCAG 2 cut(s) 1170, 1514
BshFI GGCC 2 cut(s) 866, 1452
BshVI ATCGAT 1 cut(s) 961
BsiHKAI GWGCWC 2 cut(s) 36, 429
BsiSI CCGG 4 cut(s) 249, 690, 859, 1431
BslFI GGGAC 2 cut(s) 201, 807
BslI CCNNNNNNNGG 2 cut(s) 283, 873
BsmAI GTCTC 2 cut(s) 67, 246
BsmBI CGTCTC 2 cut(s) 67, 246
BsmFI GGGAC 2 cut(s) 201, 807
BsmI GAATGC 1 cut(s) 742
BsnI GGCC 2 cut(s) 866, 1452
Bsp119I TTCGAA 1 cut(s) 1419
Bsp1286I GDGCHC 3 cut(s) 36, 429, 1141
Bsp1407I TGTACA 1 cut(s) 397
Bsp143I GATC 8 cut(s) 238, 472, 493, 574, 798, 804, 944, 1191
BspACI CCGC 4 cut(s) 504, 639, 750, 1095
BspANI GGCC 2 cut(s) 866, 1452
BspCNI CTCAG 1 cut(s) 1473
BspDI ATCGAT 1 cut(s) 961
BspLI GGNNCC 3 cut(s) 189, 643, 1006
BspMAI CTGCAG 1 cut(s) 52
BspPI GGATC 4 cut(s) 467, 501, 812, 1199
BspT104I TTCGAA 1 cut(s) 1419
BspTI CTTAAG 1 cut(s) 511
BsrFI RCCGGY 1 cut(s) 1430
BsrGI TGTACA 1 cut(s) 397
BsrI ACTGG 2 cut(s) 593, 827
BssAI RCCGGY 1 cut(s) 1430
BssMI GATC 8 cut(s) 238, 472, 493, 574, 798, 804, 944, 1191
Bst2UI CCWGG 1 cut(s) 654
Bst4CI ACNGT 3 cut(s) 440, 811, 1441
BstAFI CTTAAG 1 cut(s) 511
BstAUI TGTACA 1 cut(s) 397
BstBI TTCGAA 1 cut(s) 1419
BstC8I GCNNGC 3 cut(s) 58, 852, 856
BstDEI CTNAG 5 cut(s) 360, 371, 834, 872, 1481
BstF5I GGATG 4 cut(s) 902, 1067, 1375, 1432
BstHHI GCGC 1 cut(s) 742
BstKTI GATC 8 cut(s) 241, 475, 496, 577, 801, 807, 947, 1194
BstMAI GTCTC 2 cut(s) 67, 246
BstMBI GATC 8 cut(s) 238, 472, 493, 574, 798, 804, 944, 1191
BstMWI GCNNNNNNNGC 6 cut(s) 47, 53, 143, 636, 739, 746
BstNI CCWGG 1 cut(s) 654
BstSCI CCNGG 1 cut(s) 652
BstSFI CTRYAG 1 cut(s) 48
BstV1I GCAGC 7 cut(s) 34, 40, 62, 133, 179, 1099, 1163
BstX2I RGATCY 1 cut(s) 493
BstYI RGATCY 1 cut(s) 493
Bsu15I ATCGAT 1 cut(s) 961
Bsu36I CCTNAGG 2 cut(s) 360, 872
BsuI GTATCC 3 cut(s) 196, 442, 1382
BsuRI GGCC 2 cut(s) 866, 1452
BsuTUI ATCGAT 1 cut(s) 961
BtsCI GGATG 4 cut(s) 902, 1067, 1375, 1432
BtsIMutI CAGTG 3 cut(s) 586, 820, 1531
Cac8I GCNNGC 3 cut(s) 58, 852, 856
CaiI CAGNNNCTG 3 cut(s) 752, 983, 1157
CfoI GCGC 1 cut(s) 742
Cfr10I RCCGGY 1 cut(s) 1430
Cfr13I GGNCC 3 cut(s) 188, 345, 1092
ClaI ATCGAT 1 cut(s) 961
CseI GACGC 1 cut(s) 49
CsiI ACCWGGT 1 cut(s) 652
Csp6I GTAC 2 cut(s) 398, 1225
CviAII CATG 6 cut(s) 80, 179, 342, 920, 940, 1398
CviQI GTAC 2 cut(s) 398, 1225
DdeI CTNAG 5 cut(s) 360, 371, 834, 872, 1481
DpnI GATC 8 cut(s) 240, 474, 495, 576, 800, 806, 946, 1193
DpnII GATC 8 cut(s) 238, 472, 493, 574, 798, 804, 944, 1191
DraIII CACNNNGTG 1 cut(s) 395
EaeI YGGCCR 1 cut(s) 864
EciI GGCGGA 1 cut(s) 654
Ecl136II GAGCTC 1 cut(s) 427
Eco24I GRGCYC 2 cut(s) 429, 1141
Eco32I GATATC 1 cut(s) 234
Eco47I GGWCC 3 cut(s) 188, 345, 1092
Eco53kI GAGCTC 1 cut(s) 427
Eco57I CTGAAG 1 cut(s) 310
Eco81I CCTNAGG 2 cut(s) 360, 872
EcoICRI GAGCTC 1 cut(s) 427
EcoO109I RGGNCCY 1 cut(s) 188
EcoRII CCWGG 1 cut(s) 652
EcoRV GATATC 1 cut(s) 234
EcoT22I ATGCAT 2 cut(s) 1267, 1399
EcoT38I GRGCYC 2 cut(s) 429, 1141
Esp3I CGTCTC 2 cut(s) 67, 246
FaeI CATG 6 cut(s) 83, 182, 345, 923, 943, 1401
FalI AAGNNNNNCTT 2 cut(s) 993, 1025
FaqI GGGAC 2 cut(s) 201, 807
FatI CATG 6 cut(s) 79, 178, 341, 919, 939, 1397
FauI CCCGC 1 cut(s) 497
FauNDI CATATG 1 cut(s) 1267
Fnu4HI GCNGC 8 cut(s) 48, 51, 54, 147, 168, 750, 1113, 1152
FokI GGATG 4 cut(s) 909, 1054, 1382, 1439
FriOI GRGCYC 2 cut(s) 429, 1141
Fsp4HI GCNGC 8 cut(s) 48, 51, 54, 147, 168, 750, 1113, 1152
FspBI CTAG 2 cut(s) 38, 192
FspI TGCGCA 1 cut(s) 741
GlaI GCGC 1 cut(s) 741
GluI GCNGC 8 cut(s) 48, 51, 54, 147, 168, 750, 1113, 1152
HaeIII GGCC 2 cut(s) 866, 1452
HapII CCGG 4 cut(s) 249, 690, 859, 1431
HgaI GACGC 1 cut(s) 49
HhaI GCGC 1 cut(s) 742
Hin1II CATG 6 cut(s) 83, 182, 345, 923, 943, 1401
Hin6I GCGC 1 cut(s) 740
HinP1I GCGC 1 cut(s) 740
HincII GTYRAC 1 cut(s) 1081
HindII GTYRAC 1 cut(s) 1081
HindIII AAGCTT 1 cut(s) 780
HinfI GANTC 4 cut(s) 123, 817, 958, 986
HpaII CCGG 4 cut(s) 249, 690, 859, 1431
HphI GGTGA 4 cut(s) 266, 584, 592, 935
Hpy166II GTNNAC 3 cut(s) 385, 398, 1081
Hpy188I TCNGA 7 cut(s) 160, 238, 329, 896, 1185, 1196, 1482
Hpy188III TCNNGA 8 cut(s) 224, 454, 562, 802, 955, 1295, 1334, 1516
Hpy8I GTNNAC 3 cut(s) 385, 398, 1081
Hpy99I CGWCG 1 cut(s) 638
HpyAV CCTTC 6 cut(s) 155, 177, 334, 460, 1180, 1378
HpyCH4III ACNGT 3 cut(s) 440, 811, 1441
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 8 cut(s) 50, 650, 684, 1058, 1151, 1265, 1397, 1531
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 53, 143, 636, 739, 746
HpyF3I CTNAG 5 cut(s) 360, 371, 834, 872, 1481
HpySE526I ACGT 1 cut(s) 255
Hsp92II CATG 6 cut(s) 83, 182, 345, 923, 943, 1401
HspAI GCGC 1 cut(s) 740
Kzo9I GATC 8 cut(s) 238, 472, 493, 574, 798, 804, 944, 1191
LmnI GCTCC 4 cut(s) 39, 164, 468, 1010
Lsp1109I GCAGC 7 cut(s) 34, 40, 62, 133, 179, 1099, 1163
LweI GCATC 3 cut(s) 204, 1045, 1417
MabI ACCWGGT 1 cut(s) 652
MaeI CTAG 2 cut(s) 38, 192
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 5 cut(s) 454, 592, 814, 876, 1433
MalI GATC 8 cut(s) 240, 474, 495, 576, 800, 806, 946, 1193
MboI GATC 8 cut(s) 238, 472, 493, 574, 798, 804, 944, 1191
MboII GAAGA 3 cut(s) 316, 1064, 1497
MflI RGATCY 1 cut(s) 493
MhlI GDGCHC 3 cut(s) 36, 429, 1141
MlyI GAGTC 1 cut(s) 811
MmeI TCCRAC 4 cut(s) 297, 459, 1070, 1095
MnlI CCTC 9 cut(s) 5, 280, 371, 484, 486, 667, 1192, 1502, 1535
Mph1103I ATGCAT 2 cut(s) 1267, 1399
MseI TTAA 4 cut(s) 99, 512, 1365, 1377
MslI CAYNNNNRTG 1 cut(s) 812
MspA1I CMGCKG 4 cut(s) 53, 146, 752, 850
MspCI CTTAAG 1 cut(s) 511
MspI CCGG 4 cut(s) 249, 690, 859, 1431
MspR9I CCNGG 1 cut(s) 654
Mva1269I GAATGC 1 cut(s) 742
MvaI CCWGG 1 cut(s) 654
MwoI GCNNNNNNNGC 6 cut(s) 47, 53, 143, 636, 739, 746
NdeI CATATG 1 cut(s) 1267
NdeII GATC 8 cut(s) 238, 472, 493, 574, 798, 804, 944, 1191
NlaIII CATG 6 cut(s) 83, 182, 345, 923, 943, 1401
NlaIV GGNNCC 3 cut(s) 189, 643, 1006
NmuCI GTSAC 4 cut(s) 454, 592, 814, 876
NsbI TGCGCA 1 cut(s) 741
NsiI ATGCAT 2 cut(s) 1267, 1399
NspV TTCGAA 1 cut(s) 1419
OliI CACNNNNGTG 1 cut(s) 812
PctI GAATGC 1 cut(s) 742
PfeI GAWTC 3 cut(s) 123, 958, 986
PkrI GCNGC 8 cut(s) 49, 52, 55, 148, 169, 751, 1114, 1153
PleI GAGTC 1 cut(s) 811
PpsI GAGTC 1 cut(s) 811
PpuMI RGGWCCY 1 cut(s) 188
Psp124BI GAGCTC 1 cut(s) 429
Psp5II RGGWCCY 1 cut(s) 188
Psp6I CCWGG 1 cut(s) 652
PspGI CCWGG 1 cut(s) 652
PspN4I GGNNCC 3 cut(s) 189, 643, 1006
PspPI GGNCC 3 cut(s) 188, 345, 1092
PspPPI RGGWCCY 1 cut(s) 188
PsrI GAACNNNNNNTAC 2 cut(s) 491, 523
PstI CTGCAG 1 cut(s) 52
PstNI CAGNNNCTG 3 cut(s) 752, 983, 1157
PsuI RGATCY 1 cut(s) 493
PvuII CAGCTG 3 cut(s) 53, 146, 850
RsaI GTAC 2 cut(s) 399, 1226
RsaNI GTAC 2 cut(s) 398, 1225
RseI CAYNNNNRTG 1 cut(s) 812
SacI GAGCTC 1 cut(s) 429
SaqAI TTAA 4 cut(s) 99, 512, 1365, 1377
SatI GCNGC 8 cut(s) 48, 51, 54, 147, 168, 750, 1113, 1152
Sau3AI GATC 8 cut(s) 238, 472, 493, 574, 798, 804, 944, 1191
Sau96I GGNCC 3 cut(s) 188, 345, 1092
SchI GAGTC 1 cut(s) 811
ScrFI CCNGG 1 cut(s) 654
SduI GDGCHC 3 cut(s) 36, 429, 1141
SexAI ACCWGGT 1 cut(s) 652
SfaNI GCATC 3 cut(s) 204, 1045, 1417
SfcI CTRYAG 1 cut(s) 48
SfuI TTCGAA 1 cut(s) 1419
SinI GGWCC 3 cut(s) 188, 345, 1092
SmiMI CAYNNNNRTG 1 cut(s) 812
SmlI CTYRAG 2 cut(s) 488, 511
SmoI CTYRAG 2 cut(s) 488, 511
SsiI CCGC 4 cut(s) 504, 639, 750, 1095
SspI AATATT 1 cut(s) 1499
SspMI CTAG 2 cut(s) 38, 192
SstI GAGCTC 1 cut(s) 429
StyD4I CCNGG 1 cut(s) 652
TaaI ACNGT 3 cut(s) 440, 811, 1441
TaiI ACGT 1 cut(s) 258
TaqI TCGA 3 cut(s) 961, 989, 1419
TatI WGTACW 2 cut(s) 397, 1224
TauI GCSGC 1 cut(s) 752
TfiI GAWTC 3 cut(s) 123, 958, 986
Tru1I TTAA 4 cut(s) 99, 512, 1365, 1377
Tru9I TTAA 4 cut(s) 99, 512, 1365, 1377
TscAI CASTG 2 cut(s) 593, 827
TseFI GTSAC 4 cut(s) 454, 592, 814, 876
TseI GCWGC 7 cut(s) 47, 50, 53, 146, 167, 1112, 1151
Tsp45I GTSAC 4 cut(s) 454, 592, 814, 876
TspDTI ATGAA 5 cut(s) 317, 330, 614, 908, 1056
TspGWI ACGGA 3 cut(s) 55, 552, 627
TspRI CASTG 2 cut(s) 593, 827
Vha464I CTTAAG 1 cut(s) 511
VpaK11BI GGWCC 3 cut(s) 188, 345, 1092
XapI RAATTY 1 cut(s) 971
XspI CTAG 2 cut(s) 38, 192
Zsp2I ATGCAT 2 cut(s) 1267, 1399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.