Rh1DG004200

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0003709
Physical Location & Seq
Forward (+)
652822 .. 658581
5760 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG004200.1

Sequence Viewer

Length: 1182 bp
ATGGCATTTGGAGGTTCTGTGTCCTTGTCTATGCTCCTATCTTTGGCTGCAGCTGCTTGCTTCTCCGTTGGCACTTCCGATGCTTATTATATCCCTTTCAACCGAAGCAGTTTTCCCACTGGATTTCTTTTTGGTGCTGGTTCAGCTGCTTATCAGTCAGAAGGAGCAGCATTTCTAGATGGAAGGGGACCTAGCATCTGGGATACTTTCGTTAGGAAACATCCAGAAAAAATATGGGATCACAGCACGGGAGACGTAGCTGATGACTTTTATCACCATTATAAGGAGGATATAAAATTGATGAAGAAAATTGGGTTGGACTCTTTCAGATTCTCAATTTCATGGCCCAGATTACTTCCCAAGGGAAAACTTAGTGGAGGAGTGAACCCACTTGGTGTCAAATTCTACAATAATCTCATCAATGAGCTCGTATCCAATGGTATCAAGCCCTTGGTGACTTTGCTCCATTATGATCCTCCCCAAGCACTTGATGATCTGTATGGCGGGTTCTTAAGCCCTAACATAATAAATGATTATGGAGATTATGTGGATTTCTGCTTCAAGACATTTGGTGATCGAGTGAAGCACTGGGTCACAATGAACGAACCAAACGGATTTGCTATTAATGCCTACAGCGGCGGTACCTTTGCACCAGGTCGGTGTTCTAACTATGAAGGAAACTGCACTTCCGGTAACTCTGCGACTGAACCCTACATTGTGGCACACCATTTGCTTCTTGCTCATTCAGCTGCTGTCAAAATATACAAGGACAAATATCAGGCTTCCCAAAAGGGACAGATCGGGATTACCATAGTCACTCACTGGTGGAAACCTAAGTTCCCAGAACAGCTGGCTAGCCGGAAAGCCACCCTTAGATCACTTGCCTTTATGTTTGGATGGTTTATGGATCCTATATTTCATGGTGATTATCCGAAGGTCATGCGATCTTTGGTCGGGAATAGATTGCCGAAATTTACAGAAGCTGAATCGAAGCAGATAAAAGGGTCCCTCGATTTTCTTGGGTATAATTATTACACAACATATTATACAGTAGATGAACCGGCTTCATCCAACTCTGTCAACCATAGTTGGAGTGCAGACAGACAAATCGCTGCTTCTACTAGTATGCATCCATCGATCCCACACACAATATACAACCGGCTTAGTTATCATTCATTATAA

Protein Analysis

393

Amino Acids

43.83

Weight (kDa)

8.44

Isoelectric Point (pI)

29.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 36 - 369 4.8e-110 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 282, 1180
Acc65I GGTACC 1 cut(s) 641
AccB1I GGYRCC 1 cut(s) 641
AciI CCGC 3 cut(s) 504, 636, 639
AclWI GGATC 5 cut(s) 246, 467, 902, 915, 1132
AcsI RAATTY 2 cut(s) 401, 971
AdeI CACNNNGTG 1 cut(s) 395
AfaI GTAC 1 cut(s) 643
AfiI CCNNNNNNNGG 2 cut(s) 43, 283
AflII CTTAAG 1 cut(s) 511
AgsI TTSAA 2 cut(s) 100, 562
AhlI ACTAGT 1 cut(s) 1121
AjnI CCWGG 1 cut(s) 652
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
AluBI AGCT 7 cut(s) 53, 146, 260, 427, 749, 850, 983
AluI AGCT 7 cut(s) 53, 146, 260, 427, 749, 850, 983
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 1 cut(s) 246
AlwI GGATC 5 cut(s) 246, 467, 902, 915, 1132
AlwNI CAGNNNCTG 2 cut(s) 752, 983
AoxI GGCC 1 cut(s) 344
ApeKI GCWGC 7 cut(s) 47, 50, 53, 146, 167, 749, 1112
ApoI RAATTY 2 cut(s) 401, 971
AseI ATTAAT 1 cut(s) 624
Asp718I GGTACC 1 cut(s) 641
AspS9I GGNCC 3 cut(s) 188, 345, 1005
AsuHPI GGTGA 4 cut(s) 266, 466, 584, 935
AsuNHI GCTAGC 1 cut(s) 854
AvaII GGWCC 2 cut(s) 188, 1005
BaeI ACNNNNGTAYC 2 cut(s) 195, 228
BamHI GGATCC 1 cut(s) 907
BanI GGYRCC 1 cut(s) 641
BanII GRGCYC 1 cut(s) 429
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 7 cut(s) 34, 40, 62, 133, 179, 736, 1099
BccI CCATC 3 cut(s) 173, 891, 1141
BcgI CGANNNNNNTGC 2 cut(s) 922, 956
BciT130I CCWGG 1 cut(s) 654
BciVI GTATCC 2 cut(s) 196, 442
BcoDI GTCTC 1 cut(s) 246
BcuI ACTAGT 1 cut(s) 1121
BfaI CTAG 4 cut(s) 176, 192, 855, 1122
BfmI CTRYAG 2 cut(s) 48, 631
BfrI CTTAAG 1 cut(s) 511
BfuI GTATCC 2 cut(s) 196, 442
BisI GCNGC 8 cut(s) 48, 51, 54, 147, 168, 637, 750, 1113
BlsI GCNGC 8 cut(s) 49, 52, 55, 148, 169, 638, 751, 1114
Bme1390I CCNGG 1 cut(s) 654
Bme18I GGWCC 2 cut(s) 188, 1005
BmgT120I GGNCC 3 cut(s) 188, 345, 1005
BmiI GGNNCC 5 cut(s) 189, 643, 909, 1006, 1007
BmrFI CCNGG 1 cut(s) 654
BmrI ACTGGG 1 cut(s) 598
BmsI GCATC 3 cut(s) 70, 204, 1138
BmtI GCTAGC 1 cut(s) 858
BmuI ACTGGG 1 cut(s) 598
Bsa29I ATCGAT 1 cut(s) 1136
BsaJI CCNNGG 2 cut(s) 360, 450
BsaWI WCCGGW 1 cut(s) 689
BsaXI ACNNNNNCTCC 3 cut(s) 33, 531, 561
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 283
Bse118I RCCGGY 2 cut(s) 1060, 1158
Bse1I ACTGG 3 cut(s) 124, 593, 827
BseBI CCWGG 1 cut(s) 654
BseCI ATCGAT 1 cut(s) 1136
BseDI CCNNGG 2 cut(s) 360, 450
BseGI GGATG 4 cut(s) 220, 902, 1067, 1129
BseLI CCNNNNNNNGG 2 cut(s) 43, 283
BseNI ACTGG 3 cut(s) 124, 593, 827
BseRI GAGGAG 1 cut(s) 393
BseXI GCAGC 7 cut(s) 34, 40, 62, 133, 179, 736, 1099
BsgI GTGCAG 2 cut(s) 667, 1116
BshFI GGCC 1 cut(s) 346
BshNI GGYRCC 1 cut(s) 641
BshVI ATCGAT 1 cut(s) 1136
BsiHKAI GWGCWC 1 cut(s) 429
BsiSI CCGG 4 cut(s) 690, 859, 1061, 1159
BslFI GGGAC 3 cut(s) 201, 807, 991
BslI CCNNNNNNNGG 2 cut(s) 43, 283
BsmAI GTCTC 1 cut(s) 246
BsmBI CGTCTC 1 cut(s) 246
BsmFI GGGAC 3 cut(s) 201, 807, 991
BsnI GGCC 1 cut(s) 346
Bsp1286I GDGCHC 1 cut(s) 429
Bsp143I GATC 9 cut(s) 238, 472, 493, 574, 798, 875, 907, 944, 1137
BspACI CCGC 3 cut(s) 504, 636, 639
BspANI GGCC 1 cut(s) 346
BspDI ATCGAT 1 cut(s) 1136
BspLI GGNNCC 5 cut(s) 189, 643, 909, 1006, 1007
BspMAI CTGCAG 1 cut(s) 52
BspOI GCTAGC 1 cut(s) 858
BspPI GGATC 5 cut(s) 246, 467, 902, 915, 1132
BspT107I GGYRCC 1 cut(s) 641
BspTI CTTAAG 1 cut(s) 511
BsrFI RCCGGY 2 cut(s) 1060, 1158
BsrI ACTGG 3 cut(s) 124, 593, 827
BssAI RCCGGY 2 cut(s) 1060, 1158
BssECI CCNNGG 2 cut(s) 360, 450
BssMI GATC 9 cut(s) 238, 472, 493, 574, 798, 875, 907, 944, 1137
BssT1I CCWWGG 2 cut(s) 360, 450
Bst2UI CCWGG 1 cut(s) 654
Bst4CI ACNGT 1 cut(s) 1051
BstAFI CTTAAG 1 cut(s) 511
BstC8I GCNNGC 3 cut(s) 58, 852, 856
BstDEI CTNAG 4 cut(s) 371, 834, 872, 1163
BstF5I GGATG 4 cut(s) 220, 902, 1067, 1129
BstKTI GATC 9 cut(s) 241, 475, 496, 577, 801, 878, 910, 947, 1140
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 9 cut(s) 238, 472, 493, 574, 798, 875, 907, 944, 1137
BstMWI GCNNNNNNNGC 4 cut(s) 53, 143, 626, 746
BstNI CCWGG 1 cut(s) 654
BstSCI CCNGG 1 cut(s) 652
BstSFI CTRYAG 2 cut(s) 48, 631
BstV1I GCAGC 7 cut(s) 34, 40, 62, 133, 179, 736, 1099
BstX2I RGATCY 1 cut(s) 907
BstYI RGATCY 1 cut(s) 907
Bsu15I ATCGAT 1 cut(s) 1136
BsuI GTATCC 2 cut(s) 196, 442
BsuRI GGCC 1 cut(s) 346
BsuTUI ATCGAT 1 cut(s) 1136
BtsCI GGATG 4 cut(s) 220, 902, 1067, 1129
BtsIMutI CAGTG 3 cut(s) 117, 586, 820
Cac8I GCNNGC 3 cut(s) 58, 852, 856
CaiI CAGNNNCTG 2 cut(s) 752, 983
Cfr10I RCCGGY 2 cut(s) 1060, 1158
Cfr13I GGNCC 3 cut(s) 188, 345, 1005
ClaI ATCGAT 1 cut(s) 1136
CsiI ACCWGGT 1 cut(s) 652
Csp6I GTAC 1 cut(s) 642
CviAII CATG 3 cut(s) 342, 920, 940
CviQI GTAC 1 cut(s) 642
DdeI CTNAG 4 cut(s) 371, 834, 872, 1163
DpnI GATC 9 cut(s) 240, 474, 495, 576, 800, 877, 909, 946, 1139
DpnII GATC 9 cut(s) 238, 472, 493, 574, 798, 875, 907, 944, 1137
DraIII CACNNNGTG 1 cut(s) 395
Ecl136II GAGCTC 1 cut(s) 427
Eco130I CCWWGG 2 cut(s) 360, 450
Eco24I GRGCYC 1 cut(s) 429
Eco47I GGWCC 2 cut(s) 188, 1005
Eco53kI GAGCTC 1 cut(s) 427
EcoICRI GAGCTC 1 cut(s) 427
EcoO109I RGGNCCY 2 cut(s) 188, 1005
EcoRII CCWGG 1 cut(s) 652
EcoT14I CCWWGG 2 cut(s) 360, 450
EcoT22I ATGCAT 1 cut(s) 1131
EcoT38I GRGCYC 1 cut(s) 429
ErhI CCWWGG 2 cut(s) 360, 450
Esp3I CGTCTC 1 cut(s) 246
FaeI CATG 3 cut(s) 345, 923, 943
FalI AAGNNNNNCTT 2 cut(s) 855, 887
FaqI GGGAC 3 cut(s) 201, 807, 991
FatI CATG 3 cut(s) 341, 919, 939
FauI CCCGC 1 cut(s) 497
Fnu4HI GCNGC 8 cut(s) 48, 51, 54, 147, 168, 637, 750, 1113
FokI GGATG 4 cut(s) 207, 909, 1054, 1116
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 8 cut(s) 48, 51, 54, 147, 168, 637, 750, 1113
FspBI CTAG 4 cut(s) 176, 192, 855, 1122
GluI GCNGC 8 cut(s) 48, 51, 54, 147, 168, 637, 750, 1113
HaeIII GGCC 1 cut(s) 346
HapII CCGG 4 cut(s) 690, 859, 1061, 1159
Hin1II CATG 3 cut(s) 345, 923, 943
HincII GTYRAC 1 cut(s) 1081
HindII GTYRAC 1 cut(s) 1081
HinfI GANTC 3 cut(s) 320, 330, 986
HpaII CCGG 4 cut(s) 690, 859, 1061, 1159
HphI GGTGA 4 cut(s) 266, 466, 584, 935
Hpy166II GTNNAC 2 cut(s) 385, 1081
Hpy188I TCNGA 4 cut(s) 79, 160, 329, 933
Hpy188III TCNNGA 5 cut(s) 176, 224, 562, 802, 955
Hpy8I GTNNAC 2 cut(s) 385, 1081
HpyAV CCTTC 4 cut(s) 155, 177, 668, 928
HpyCH4III ACNGT 1 cut(s) 1051
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 5 cut(s) 50, 650, 684, 1097, 1129
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 143, 626, 746
HpyF3I CTNAG 4 cut(s) 371, 834, 872, 1163
HpySE526I ACGT 1 cut(s) 255
Hsp92II CATG 3 cut(s) 345, 923, 943
KflI GGGWCCC 1 cut(s) 1005
KpnI GGTACC 1 cut(s) 645
Kzo9I GATC 9 cut(s) 238, 472, 493, 574, 798, 875, 907, 944, 1137
LmnI GCTCC 3 cut(s) 39, 164, 468
Lsp1109I GCAGC 7 cut(s) 34, 40, 62, 133, 179, 736, 1099
LweI GCATC 3 cut(s) 70, 204, 1138
MabI ACCWGGT 1 cut(s) 652
MaeI CTAG 4 cut(s) 176, 192, 855, 1122
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 4 cut(s) 454, 592, 692, 814
MalI GATC 9 cut(s) 240, 474, 495, 576, 800, 877, 909, 946, 1139
MboI GATC 9 cut(s) 238, 472, 493, 574, 798, 875, 907, 944, 1137
MboII GAAGA 1 cut(s) 316
MflI RGATCY 1 cut(s) 907
MhlI GDGCHC 1 cut(s) 429
MluCI AATT 6 cut(s) 296, 309, 336, 401, 971, 1027
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 3 cut(s) 297, 1070, 1095
MnlI CCTC 5 cut(s) 5, 280, 371, 486, 1019
Mph1103I ATGCAT 1 cut(s) 1131
MseI TTAA 2 cut(s) 512, 624
MspA1I CMGCKG 5 cut(s) 53, 146, 636, 749, 850
MspCI CTTAAG 1 cut(s) 511
MspI CCGG 4 cut(s) 690, 859, 1061, 1159
MspR9I CCNGG 1 cut(s) 654
MvaI CCWGG 1 cut(s) 654
MwoI GCNNNNNNNGC 4 cut(s) 53, 143, 626, 746
NdeII GATC 9 cut(s) 238, 472, 493, 574, 798, 875, 907, 944, 1137
NheI GCTAGC 1 cut(s) 854
NlaIII CATG 3 cut(s) 345, 923, 943
NlaIV GGNNCC 5 cut(s) 189, 643, 909, 1006, 1007
NmuCI GTSAC 3 cut(s) 454, 592, 814
NsiI ATGCAT 1 cut(s) 1131
PfeI GAWTC 2 cut(s) 330, 986
PkrI GCNGC 8 cut(s) 49, 52, 55, 148, 169, 638, 751, 1114
PleI GAGTC 1 cut(s) 314
PpsI GAGTC 1 cut(s) 314
PpuMI RGGWCCY 2 cut(s) 188, 1005
PshBI ATTAAT 1 cut(s) 624
PsiI TTATAA 2 cut(s) 282, 1180
Psp124BI GAGCTC 1 cut(s) 429
Psp5II RGGWCCY 2 cut(s) 188, 1005
Psp6I CCWGG 1 cut(s) 652
PspGI CCWGG 1 cut(s) 652
PspN4I GGNNCC 5 cut(s) 189, 643, 909, 1006, 1007
PspPI GGNCC 3 cut(s) 188, 345, 1005
PspPPI RGGWCCY 2 cut(s) 188, 1005
PsrI GAACNNNNNNTAC 2 cut(s) 491, 523
PstI CTGCAG 1 cut(s) 52
PstNI CAGNNNCTG 2 cut(s) 752, 983
PsuI RGATCY 1 cut(s) 907
PvuII CAGCTG 4 cut(s) 53, 146, 749, 850
RsaI GTAC 1 cut(s) 643
RsaNI GTAC 1 cut(s) 642
SacI GAGCTC 1 cut(s) 429
SaqAI TTAA 2 cut(s) 512, 624
SatI GCNGC 8 cut(s) 48, 51, 54, 147, 168, 637, 750, 1113
Sau3AI GATC 9 cut(s) 238, 472, 493, 574, 798, 875, 907, 944, 1137
Sau96I GGNCC 3 cut(s) 188, 345, 1005
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 1 cut(s) 654
SduI GDGCHC 1 cut(s) 429
SexAI ACCWGGT 1 cut(s) 652
SfaNI GCATC 3 cut(s) 70, 204, 1138
SfcI CTRYAG 2 cut(s) 48, 631
SinI GGWCC 2 cut(s) 188, 1005
SmlI CTYRAG 1 cut(s) 511
SmoI CTYRAG 1 cut(s) 511
SpeI ACTAGT 1 cut(s) 1121
Sse9I AATT 6 cut(s) 296, 309, 336, 401, 971, 1027
SsiI CCGC 3 cut(s) 504, 636, 639
SspMI CTAG 4 cut(s) 176, 192, 855, 1122
SstI GAGCTC 1 cut(s) 429
StyD4I CCNGG 1 cut(s) 652
StyI CCWWGG 2 cut(s) 360, 450
TaaI ACNGT 1 cut(s) 1051
TaiI ACGT 1 cut(s) 258
TaqI TCGA 4 cut(s) 577, 989, 1011, 1136
TasI AATT 6 cut(s) 296, 309, 336, 401, 971, 1027
TauI GCSGC 1 cut(s) 639
TfiI GAWTC 2 cut(s) 330, 986
Tru1I TTAA 2 cut(s) 512, 624
Tru9I TTAA 2 cut(s) 512, 624
TscAI CASTG 3 cut(s) 124, 593, 827
TseFI GTSAC 3 cut(s) 454, 592, 814
TseI GCWGC 7 cut(s) 47, 50, 53, 146, 167, 749, 1112
Tsp45I GTSAC 3 cut(s) 454, 592, 814
TspDTI ATGAA 8 cut(s) 317, 330, 614, 687, 908, 1056, 1071, 1164
TspGWI ACGGA 2 cut(s) 55, 627
TspRI CASTG 3 cut(s) 124, 593, 827
Vha464I CTTAAG 1 cut(s) 511
VpaK11BI GGWCC 2 cut(s) 188, 1005
VspI ATTAAT 1 cut(s) 624
XapI RAATTY 2 cut(s) 401, 971
XbaI TCTAGA 1 cut(s) 175
XcmI CCANNNNNNNNNTGG 1 cut(s) 231
XspI CTAG 4 cut(s) 176, 192, 855, 1122
Zsp2I ATGCAT 1 cut(s) 1131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.