FvH4_7g09111

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0005196
Physical Location & Seq
Forward (+)
8847581 .. 8851609
4029 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g09111.t2

Sequence Viewer

Length: 1554 bp
ATGGCAGCAAAGGTGATACTATCAGTGTTGTTGGGTGGGTTTGTGATAGGGGTACTGTATCTCTTTGAGGTTTTGGTGCTGCAGCCAAAGAGGCTAAGATCAAAGCTGGAAAAGCAAGGGATCAGAGGGCCTCCTCCAACGATTCTATTGGGGAATATCCCAGAAATGAAGAAGATCAAACTCGAGTCCATGAAGAAGTCAGAAGCAAAGGATCAGACACCTTCTATTGCTCATGATTGGCCTTCAAAACTCTTCCCTCATATTGCGCAGTGGAGAAAGGAATACGGGCCAATCTTCACCTATTCAACTGGAATCATACAGCTATTAAGCGTTACGGAGATAGAGATGGTGAAGGAAATTAGTATGTGCACGTCTTTGATATTAGGAAGGCCTGCTTATATGTCTAAGGATTTGAAGCCTCTATTAGGACAAGGCATTATCTCTTCAAGTGGCCAAACTTGGTCACACCAGAGGAAAATTATTTCTCCTGAATTCTACTCTGATAAGGTTAAGGGTATGGTCGATTTAATGGTGGACTCTACAGCCTTAATGTTAAGATTTTGGGAAAGCATAGTTGAAAGTGATGGAGGAATTGCACGGCTTAGGATTGATGTATATTTGAGAAGCTTGTCAGCAGATATAATATCAAGGTCATCCTTTGGGAGTAATTATGTAAAAGGGAAACAAATTTTTTTAAAGCTCAGGACTCTTCAACAACTTATGTCTCAGGGAAACCTTGGCATTCCTGGGTTAAGGCACTTGCCAACAAAGAGGAACAAAGAAATAAGGAGTTTAGAGAAAGAGATTCACTCAATGATATTGGAGGTAGCAAAAAAACGCAATGAGGCTACCTCTGAGAAGGATCTTCTTCAAATGATACTGGAGGGAGCAAAAAATTACTCTGACGCTGATAGCTTTTTTTCATCTGGTACATCTGAGGATCAGTTCATAGTGGATAATTGCAAGAATATATACTTTGCTGGCCATGAAACCACTGCCATCACAGCATCATGGTGCTTGCTGTTGCTGGCTGCATATCCGGAGTGGCAAGCTTGTGTTCGTGCTGAGGTGCTTGAAATTTGCAAGGATGGCATTGTAAATGCTGATGTGCTTCGAGGCATGAAAACATTGACTATGGTGATCCAAGAAACATTGCGACTGTACCCACCAGCAACTTTTATCGTAAGACATGCATTGGAAGATATTAAACTCAGAGGCATTCTAATTCCCAAGGGAACTACCATTCAGATCCCAATCTCAATGGTGCAGCAATTGCCTGAATTATGGGGACCTGATGCTGCTGACTTCAATCCCATGAGATTCGAGGATGGAATTCGTGGGGCTTGCAAGTTTCCTCAGGCCTATTTCCCATTTGGAGTTGGCGCTCGCATTTGTGTTGGCCAAAACTTAGCAATGACAGAATTGAAGGTGATTTTGTCTCTCCTTCTATCCAAGTTATCTTTCTCGCTCGATCCTGAATACAAACACAGCCCGGTTTTTAAGTTGGTTACTGAGCCTGAACATGGAGTAACTCTATTTGTGAGGAGGGTGTGA

Protein Analysis

518

Amino Acids

58.03

Weight (kDa)

9.12

Isoelectric Point (pI)

45.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 86 - 363 1.6e-31 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 267
AccIII TCCGGA 1 cut(s) 1039
AclWI GGATC 7 cut(s) 128, 219, 870, 948, 1135, 1243, 1466
AcoI YGGCCR 3 cut(s) 451, 982, 1399
AcsI RAATTY 4 cut(s) 491, 687, 1077, 1332
AfaI GTAC 3 cut(s) 54, 931, 1163
AfiI CCNNNNNNNGG 2 cut(s) 425, 1523
AjiI CACGTC 1 cut(s) 372
AjnI CCWGG 1 cut(s) 745
AjuI GAANNNNNNNTTGG 2 cut(s) 1445, 1477
AluBI AGCT 6 cut(s) 106, 322, 627, 700, 915, 1052
AluI AGCT 6 cut(s) 106, 322, 627, 700, 915, 1052
Alw21I GWGCWC 1 cut(s) 371
Alw26I GTCTC 2 cut(s) 729, 1443
Alw44I GTGCAC 1 cut(s) 367
AlwI GGATC 7 cut(s) 128, 219, 870, 948, 1135, 1243, 1466
Ama87I CYCGRG 1 cut(s) 182
Aor13HI TCCGGA 1 cut(s) 1039
AoxI GGCC 8 cut(s) 128, 239, 287, 389, 451, 982, 1359, 1399
ApaLI GTGCAC 1 cut(s) 367
ApeKI GCWGC 6 cut(s) 5, 79, 82, 1031, 1267, 1298
ApoI RAATTY 4 cut(s) 491, 687, 1077, 1332
AspLEI GCGC 2 cut(s) 268, 1385
AspS9I GGNCC 3 cut(s) 128, 287, 1289
AsuC2I CCSGG 1 cut(s) 1493
AsuHPI GGTGA 5 cut(s) 25, 289, 361, 1150, 1441
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 1 cut(s) 1289
AxyI CCTNAGG 1 cut(s) 1356
BaeGI GKGCMC 1 cut(s) 371
BalI TGGCCA 3 cut(s) 453, 984, 1401
Bbv12I GWGCWC 1 cut(s) 371
BbvCI CCTCAGC 1 cut(s) 1065
BbvI GCAGC 6 cut(s) 17, 66, 94, 1018, 1279, 1285
BccI CCATC 5 cut(s) 340, 578, 1007, 1082, 1322
BceAI ACGGC 1 cut(s) 614
BciT130I CCWGG 1 cut(s) 747
BcnI CCSGG 1 cut(s) 1493
BcoDI GTCTC 2 cut(s) 729, 1443
BfmI CTRYAG 2 cut(s) 80, 540
BfoI RGCGCY 1 cut(s) 1386
BglI GCCNNNNNGGC 1 cut(s) 91
BisI GCNGC 6 cut(s) 6, 80, 83, 1032, 1268, 1299
BlsI GCNGC 6 cut(s) 7, 81, 84, 1033, 1269, 1300
Bme1390I CCNGG 2 cut(s) 747, 1493
Bme18I GGWCC 1 cut(s) 1289
BmeT110I CYCGRG 1 cut(s) 182
BmgBI CACGTC 1 cut(s) 372
BmgT120I GGNCC 3 cut(s) 128, 287, 1289
BmiI GGNNCC 1 cut(s) 1290
BmrFI CCNGG 2 cut(s) 747, 1493
BmsI GCATC 2 cut(s) 1016, 1285
BplI GAGNNNNNCTC 4 cut(s) 794, 826, 836, 868
BpmI CTGGAG 1 cut(s) 902
Bpu10I CCTNAGC 3 cut(s) 602, 701, 1065
BpuMI CCSGG 1 cut(s) 1493
BsaBI GATNNNNATC 1 cut(s) 1253
BsaJI CCNNGG 3 cut(s) 736, 746, 1230
BsaWI WCCGGW 1 cut(s) 1039
Bsc4I CCNNNNNNNGG 2 cut(s) 425, 1523
Bse1I ACTGG 2 cut(s) 313, 885
Bse21I CCTNAGG 1 cut(s) 1356
Bse3DI GCAATG 3 cut(s) 847, 1151, 1419
Bse8I GATNNNNATC 1 cut(s) 1253
BseAI TCCGGA 1 cut(s) 1039
BseBI CCWGG 1 cut(s) 747
BseDI CCNNGG 3 cut(s) 736, 746, 1230
BseGI GGATG 3 cut(s) 653, 1093, 1333
BseJI GATNNNNATC 1 cut(s) 1253
BseLI CCNNNNNNNGG 2 cut(s) 425, 1523
BseMI GCAATG 3 cut(s) 847, 1151, 1419
BseMII CTCAG 8 cut(s) 715, 740, 846, 927, 1056, 1225, 1370, 1503
BseNI ACTGG 2 cut(s) 313, 885
BseRI GAGGAG 1 cut(s) 123
BseSI GKGCMC 1 cut(s) 371
BseXI GCAGC 6 cut(s) 17, 66, 94, 1018, 1279, 1285
BsgI GTGCAG 1 cut(s) 1286
BshFI GGCC 8 cut(s) 130, 241, 289, 391, 453, 984, 1361, 1401
BsiHKAI GWGCWC 1 cut(s) 371
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 2 cut(s) 1040, 1493
BslFI GGGAC 1 cut(s) 1302
BslI CCNNNNNNNGG 2 cut(s) 425, 1523
BsmAI GTCTC 2 cut(s) 729, 1443
BsmFI GGGAC 1 cut(s) 1302
BsmI GAATGC 2 cut(s) 741, 1218
BsnI GGCC 8 cut(s) 130, 241, 289, 391, 453, 984, 1361, 1401
BsoBI CYCGRG 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 371
Bsp13I TCCGGA 1 cut(s) 1039
Bsp143I GATC 9 cut(s) 98, 120, 174, 211, 862, 940, 1140, 1248, 1471
BspANI GGCC 8 cut(s) 130, 241, 289, 391, 453, 984, 1361, 1401
BspCNI CTCAG 8 cut(s) 714, 739, 847, 928, 1057, 1224, 1369, 1504
BspEI TCCGGA 1 cut(s) 1039
BspHI TCATGA 1 cut(s) 232
BspLI GGNNCC 1 cut(s) 1290
BspMAI CTGCAG 1 cut(s) 84
BspPI GGATC 7 cut(s) 128, 219, 870, 948, 1135, 1243, 1466
BsrDI GCAATG 3 cut(s) 847, 1151, 1419
BsrI ACTGG 2 cut(s) 313, 885
BssECI CCNNGG 3 cut(s) 736, 746, 1230
BssMI GATC 9 cut(s) 98, 120, 174, 211, 862, 940, 1140, 1248, 1471
BssT1I CCWWGG 2 cut(s) 736, 1230
Bst2UI CCWGG 1 cut(s) 747
Bst4CI ACNGT 2 cut(s) 57, 1161
Bst6I CTCTTC 3 cut(s) 257, 448, 714
BstAPI GCANNNNNTGC 1 cut(s) 1273
BstC8I GCNNGC 7 cut(s) 393, 982, 1019, 1029, 1050, 1345, 1387
BstENI CCTNNNNNAGG 1 cut(s) 423
BstF5I GGATG 3 cut(s) 653, 1093, 1333
BstH2I RGCGCY 1 cut(s) 1386
BstHHI GCGC 2 cut(s) 268, 1385
BstKTI GATC 9 cut(s) 101, 123, 177, 214, 865, 943, 1143, 1251, 1474
BstMAI GTCTC 2 cut(s) 729, 1443
BstMBI GATC 9 cut(s) 98, 120, 174, 211, 862, 940, 1140, 1248, 1471
BstMWI GCNNNNNNNGC 5 cut(s) 91, 112, 1004, 1089, 1273
BstNI CCWGG 1 cut(s) 747
BstNSI RCATGY 1 cut(s) 1193
BstSCI CCNGG 2 cut(s) 745, 1491
BstSFI CTRYAG 2 cut(s) 80, 540
BstSLI GKGCMC 1 cut(s) 371
BstV1I GCAGC 6 cut(s) 17, 66, 94, 1018, 1279, 1285
BstX2I RGATCY 2 cut(s) 862, 1248
BstYI RGATCY 2 cut(s) 862, 1248
Bsu36I CCTNAGG 1 cut(s) 1356
BsuRI GGCC 8 cut(s) 130, 241, 289, 391, 453, 984, 1361, 1401
BtrI CACGTC 1 cut(s) 372
BtsCI GGATG 3 cut(s) 653, 1093, 1333
BtsI GCAGTG 2 cut(s) 275, 993
BtsIMutI CAGTG 3 cut(s) 30, 275, 993
Cac8I GCNNGC 7 cut(s) 393, 982, 1019, 1029, 1050, 1345, 1387
CciI TCATGA 1 cut(s) 232
CfoI GCGC 2 cut(s) 268, 1385
Cfr13I GGNCC 3 cut(s) 128, 287, 1289
CseI GACGC 1 cut(s) 914
Csp6I GTAC 3 cut(s) 53, 930, 1162
CviAII CATG 8 cut(s) 190, 233, 986, 1011, 1120, 1190, 1315, 1523
CviQI GTAC 3 cut(s) 53, 930, 1162
DpnI GATC 9 cut(s) 100, 122, 176, 213, 864, 942, 1142, 1250, 1473
DpnII GATC 9 cut(s) 98, 120, 174, 211, 862, 940, 1140, 1248, 1471
DraI TTTAAA 1 cut(s) 696
EaeI YGGCCR 3 cut(s) 451, 982, 1399
Eam1104I CTCTTC 3 cut(s) 257, 448, 714
EarI CTCTTC 3 cut(s) 257, 448, 714
Eco130I CCWWGG 2 cut(s) 736, 1230
Eco147I AGGCCT 2 cut(s) 391, 1361
Eco47I GGWCC 1 cut(s) 1289
Eco81I CCTNAGG 1 cut(s) 1356
Eco88I CYCGRG 1 cut(s) 182
EcoNI CCTNNNNNAGG 1 cut(s) 423
EcoO109I RGGNCCY 2 cut(s) 128, 1289
EcoRI GAATTC 2 cut(s) 491, 1332
EcoRII CCWGG 1 cut(s) 745
EcoT14I CCWWGG 2 cut(s) 736, 1230
EcoT22I ATGCAT 1 cut(s) 1195
ErhI CCWWGG 2 cut(s) 736, 1230
FaeI CATG 8 cut(s) 193, 236, 989, 1014, 1123, 1193, 1318, 1526
FalI AAGNNNNNCTT 2 cut(s) 379, 411
FaqI GGGAC 1 cut(s) 1302
FatI CATG 8 cut(s) 189, 232, 985, 1010, 1119, 1189, 1314, 1522
Fnu4HI GCNGC 6 cut(s) 6, 80, 83, 1032, 1268, 1299
FokI GGATG 3 cut(s) 640, 1100, 1340
Fsp4HI GCNGC 6 cut(s) 6, 80, 83, 1032, 1268, 1299
FspI TGCGCA 1 cut(s) 267
GlaI GCGC 2 cut(s) 267, 1384
GluI GCNGC 6 cut(s) 6, 80, 83, 1032, 1268, 1299
GsuI CTGGAG 1 cut(s) 902
HaeII RGCGCY 1 cut(s) 1386
HaeIII GGCC 8 cut(s) 130, 241, 289, 391, 453, 984, 1361, 1401
HapII CCGG 2 cut(s) 1040, 1493
HgaI GACGC 1 cut(s) 914
HhaI GCGC 2 cut(s) 268, 1385
Hin1II CATG 8 cut(s) 193, 236, 989, 1014, 1123, 1193, 1318, 1526
Hin6I GCGC 2 cut(s) 266, 1383
HinP1I GCGC 2 cut(s) 266, 1383
HindIII AAGCTT 2 cut(s) 625, 1050
HinfI GANTC 7 cut(s) 142, 185, 312, 536, 706, 805, 1320
HpaII CCGG 2 cut(s) 1040, 1493
HphI GGTGA 5 cut(s) 25, 289, 361, 1150, 1441
Hpy166II GTNNAC 2 cut(s) 369, 535
Hpy188I TCNGA 9 cut(s) 125, 202, 216, 502, 856, 904, 937, 1214, 1248
Hpy188III TCNNGA 5 cut(s) 233, 488, 703, 1040, 1475
Hpy8I GTNNAC 2 cut(s) 369, 535
HpyAV CCTTC 7 cut(s) 231, 252, 346, 381, 853, 1420, 1454
HpyCH4III ACNGT 2 cut(s) 57, 1161
HpyCH4IV ACGT 1 cut(s) 371
HpyCH4V TGCA 9 cut(s) 82, 369, 596, 963, 1034, 1083, 1193, 1267, 1347
HpyF10VI GCNNNNNNNGC 5 cut(s) 91, 112, 1004, 1089, 1273
HpySE526I ACGT 1 cut(s) 371
Hsp92II CATG 8 cut(s) 193, 236, 989, 1014, 1123, 1193, 1318, 1526
HspAI GCGC 2 cut(s) 266, 1383
Kpn2I TCCGGA 1 cut(s) 1039
Kzo9I GATC 9 cut(s) 98, 120, 174, 211, 862, 940, 1140, 1248, 1471
LmnI GCTCC 1 cut(s) 887
Lsp1109I GCAGC 6 cut(s) 17, 66, 94, 1018, 1279, 1285
LweI GCATC 2 cut(s) 1016, 1285
MaeII ACGT 1 cut(s) 371
MaeIII GTNAC 4 cut(s) 331, 462, 1507, 1528
MalI GATC 9 cut(s) 100, 122, 176, 213, 864, 942, 1142, 1250, 1473
MboI GATC 9 cut(s) 98, 120, 174, 211, 862, 940, 1140, 1248, 1471
MfeI CAATTG 1 cut(s) 1271
MflI RGATCY 2 cut(s) 862, 1248
MhlI GDGCHC 1 cut(s) 371
MlsI TGGCCA 3 cut(s) 453, 984, 1401
MluNI TGGCCA 3 cut(s) 453, 984, 1401
MlyI GAGTC 3 cut(s) 194, 530, 700
MmeI TCCRAC 1 cut(s) 161
Mox20I TGGCCA 3 cut(s) 453, 984, 1401
Mph1103I ATGCAT 1 cut(s) 1195
MroI TCCGGA 1 cut(s) 1039
MscI TGGCCA 3 cut(s) 453, 984, 1401
MseI TTAA 9 cut(s) 326, 510, 527, 548, 554, 695, 752, 1206, 1500
MslI CAYNNNNRTG 1 cut(s) 1012
Msp20I TGGCCA 3 cut(s) 453, 984, 1401
MspI CCGG 2 cut(s) 1040, 1493
MspR9I CCNGG 2 cut(s) 747, 1493
MunI CAATTG 1 cut(s) 1271
Mva1269I GAATGC 2 cut(s) 741, 1218
MvaI CCWGG 1 cut(s) 747
MwoI GCNNNNNNNGC 5 cut(s) 91, 112, 1004, 1089, 1273
NciI CCSGG 1 cut(s) 1493
NdeII GATC 9 cut(s) 98, 120, 174, 211, 862, 940, 1140, 1248, 1471
NlaIII CATG 8 cut(s) 193, 236, 989, 1014, 1123, 1193, 1318, 1526
NlaIV GGNNCC 1 cut(s) 1290
NmuCI GTSAC 1 cut(s) 462
NsbI TGCGCA 1 cut(s) 267
NsiI ATGCAT 1 cut(s) 1195
NspI RCATGY 1 cut(s) 1193
PaeR7I CTCGAG 1 cut(s) 182
PagI TCATGA 1 cut(s) 232
PceI AGGCCT 2 cut(s) 391, 1361
PctI GAATGC 2 cut(s) 741, 1218
PfeI GAWTC 4 cut(s) 142, 312, 805, 1320
PkrI GCNGC 6 cut(s) 7, 81, 84, 1033, 1269, 1300
PleI GAGTC 3 cut(s) 193, 530, 700
PpsI GAGTC 3 cut(s) 193, 530, 700
PpuMI RGGWCCY 1 cut(s) 1289
Psp5II RGGWCCY 1 cut(s) 1289
Psp6I CCWGG 1 cut(s) 745
PspGI CCWGG 1 cut(s) 745
PspN4I GGNNCC 1 cut(s) 1290
PspPI GGNCC 3 cut(s) 128, 287, 1289
PspPPI RGGWCCY 1 cut(s) 1289
PspXI VCTCGAGB 1 cut(s) 182
PstI CTGCAG 1 cut(s) 84
PsuI RGATCY 2 cut(s) 862, 1248
RsaI GTAC 3 cut(s) 54, 931, 1163
RsaNI GTAC 3 cut(s) 53, 930, 1162
RseI CAYNNNNRTG 1 cut(s) 1012
SaqAI TTAA 9 cut(s) 326, 510, 527, 548, 554, 695, 752, 1206, 1500
SatI GCNGC 6 cut(s) 6, 80, 83, 1032, 1268, 1299
Sau3AI GATC 9 cut(s) 98, 120, 174, 211, 862, 940, 1140, 1248, 1471
Sau96I GGNCC 3 cut(s) 128, 287, 1289
SchI GAGTC 3 cut(s) 194, 530, 700
ScrFI CCNGG 2 cut(s) 747, 1493
SduI GDGCHC 1 cut(s) 371
SfaNI GCATC 2 cut(s) 1016, 1285
SfcI CTRYAG 2 cut(s) 80, 540
Sfr274I CTCGAG 1 cut(s) 182
SinI GGWCC 1 cut(s) 1289
SlaI CTCGAG 1 cut(s) 182
SmiMI CAYNNNNRTG 1 cut(s) 1012
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
SseBI AGGCCT 2 cut(s) 391, 1361
StuI AGGCCT 2 cut(s) 391, 1361
StyD4I CCNGG 2 cut(s) 745, 1491
StyI CCWWGG 2 cut(s) 736, 1230
TaaI ACNGT 2 cut(s) 57, 1161
TaiI ACGT 1 cut(s) 374
TaqI TCGA 5 cut(s) 183, 522, 1114, 1323, 1470
TfiI GAWTC 4 cut(s) 142, 312, 805, 1320
Tru1I TTAA 9 cut(s) 326, 510, 527, 548, 554, 695, 752, 1206, 1500
Tru9I TTAA 9 cut(s) 326, 510, 527, 548, 554, 695, 752, 1206, 1500
TscAI CASTG 3 cut(s) 30, 275, 1000
TseFI GTSAC 1 cut(s) 462
TseI GCWGC 6 cut(s) 5, 79, 82, 1031, 1267, 1298
Tsp45I GTSAC 1 cut(s) 462
TspDTI ATGAA 6 cut(s) 182, 206, 912, 937, 1002, 1136
TspGWI ACGGA 1 cut(s) 350
TspRI CASTG 3 cut(s) 30, 275, 1000
VneI GTGCAC 1 cut(s) 367
VpaK11BI GGWCC 1 cut(s) 1289
XagI CCTNNNNNAGG 1 cut(s) 423
XapI RAATTY 4 cut(s) 491, 687, 1077, 1332
XceI RCATGY 1 cut(s) 1193
XhoI CTCGAG 1 cut(s) 182
Zsp2I ATGCAT 1 cut(s) 1195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.