Rh1BG159900

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0005196
Physical Location & Seq
Forward (+)
25863076 .. 25864629
1554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG159900.1

Sequence Viewer

Length: 1038 bp
ATGGTCGATTTAATGGTGGACTCTACAGCGTCACTATTAAGATCTTGGGAAAGCAAAGTTGAAAGTGAGGGAGGAATGGCATTGTTTAGGATTGATCAAGATTTGAGAAGCTTGTCAGCAGATATAATATCAAGATCATCCTTTGGAAGTAATTATTCAAAGGGGAAAGAAATCTTTTCAAAGCTCAGGACTCTTCAACAAGTTATGTCTCAGGGAAACCTTGGCATTCCCGGCTTAAGGCACTTGCCAACAAAGAGGAACAGAGAGATAAGGAGTCTAGAGAAAGAGATTCACTCAATGATACTGGAGGTTGCCAAAAAGCGCAATGAGGCTACTTATGAGAAGGATCTTCTTCAAATGATACTCGAGGGAGCAAAAAATTACTCTGATGCTGATAGCTTTTTTTCATCTGGTATGTCCAAGGACCAGTTCATAGTGGATAATTGCAAGAATATATATTTTGCTGGCCATGAGACCACCGCCATCACAGCATCATGGTGCTTGCTGTTGTTGGCTGCATATCCAGAGTGGCAAGCTCGTGTTCGTGCTGAGGTGCTTGAAATTTGCAAGGATGGCATTCCAAATGCTGATGTGCTTCGAGGCATGAAAACATTGACTATGGTGATTCAAGAAACACTGCGCTTGTACTCACCAGCAACTTTCATCGTAAGACATGCATTGGAAGATATTAAACTTAAAGACATTCTAATTCCCAAAGGAACCACCATTCAGATCCCAATCTCAATGGTGCAGCAATTGCCGGAATTATGGGGACCTGATGCTGCTGACTTTAATCCCAATAGATTCGAGGATGGAATTCGTGGGGCTTGCAAGTTTCCTCAGGCCTATTTCCCATTTGGATTTGGCGCTCGCATTTGTGTTGGCCAAAACTTGGCCATGACAGAATTGAAGGTGATTCTGTCTCTCATATTATCCAAGTTATCATTCTCGCTCGACCCTGCATACCAACACAGCCCGGTTTTTAGGTTGGTTATTGAACCTGAACATGGAGTAAGTCTCCATGTGAGGAGGGTGTGA

Protein Analysis

345

Amino Acids

38.83

Weight (kDa)

7.66

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 1 - 329 3.4e-64 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 892
AciI CCGC 1 cut(s) 480
AclWI GGATC 2 cut(s) 354, 727
AcoI YGGCCR 3 cut(s) 466, 883, 894
AcsI RAATTY 2 cut(s) 561, 816
AfaI GTAC 1 cut(s) 647
AfiI CCNNNNNNNGG 3 cut(s) 237, 892, 1007
AflII CTTAAG 1 cut(s) 235
AgsI TTSAA 9 cut(s) 62, 159, 180, 197, 356, 560, 629, 910, 998
AjuI GAANNNNNNNTTGG 4 cut(s) 413, 445, 929, 961
AluBI AGCT 4 cut(s) 111, 184, 399, 536
AluI AGCT 4 cut(s) 111, 184, 399, 536
Alw26I GTCTC 4 cut(s) 213, 467, 927, 1022
AlwI GGATC 2 cut(s) 354, 727
Ama87I CYCGRG 1 cut(s) 365
AoxI GGCC 4 cut(s) 466, 843, 883, 894
ApeKI GCWGC 3 cut(s) 515, 751, 782
ApoI RAATTY 2 cut(s) 561, 816
AspLEI GCGC 3 cut(s) 324, 642, 869
AspS9I GGNCC 2 cut(s) 424, 773
AsuC2I CCSGG 2 cut(s) 231, 977
AsuHPI GGTGA 3 cut(s) 634, 642, 925
AvaI CYCGRG 1 cut(s) 365
AvaII GGWCC 2 cut(s) 424, 773
AxyI CCTNAGG 1 cut(s) 840
BalI TGGCCA 3 cut(s) 468, 885, 896
BauI CACGAG 1 cut(s) 537
BbvCI CCTCAGC 1 cut(s) 549
BbvI GCAGC 3 cut(s) 502, 763, 769
BccI CCATC 3 cut(s) 491, 566, 806
BclI TGATCA 1 cut(s) 94
BcnI CCSGG 2 cut(s) 231, 977
BcoDI GTCTC 4 cut(s) 213, 467, 927, 1022
BfaI CTAG 1 cut(s) 278
BfmI CTRYAG 1 cut(s) 24
BfoI RGCGCY 1 cut(s) 870
BfrI CTTAAG 1 cut(s) 235
BglII AGATCT 1 cut(s) 41
BisI GCNGC 3 cut(s) 516, 752, 783
BlsI GCNGC 3 cut(s) 517, 753, 784
Bme1390I CCNGG 2 cut(s) 231, 977
Bme18I GGWCC 2 cut(s) 424, 773
BmeT110I CYCGRG 1 cut(s) 365
BmgT120I GGNCC 2 cut(s) 424, 773
BmiI GGNNCC 2 cut(s) 721, 774
BmrFI CCNGG 2 cut(s) 231, 977
BmsI GCATC 3 cut(s) 379, 500, 769
BplI GAGNNNNNCTC 4 cut(s) 278, 310, 1002, 1034
BpmI CTGGAG 1 cut(s) 326
Bpu10I CCTNAGC 2 cut(s) 185, 549
BpuMI CCSGG 2 cut(s) 231, 977
BsaBI GATNNNNATC 1 cut(s) 737
BsaI GGTCTC 1 cut(s) 467
BsaJI CCNNGG 2 cut(s) 220, 420
Bsc4I CCNNNNNNNGG 3 cut(s) 237, 892, 1007
Bse1I ACTGG 2 cut(s) 309, 427
Bse21I CCTNAGG 1 cut(s) 840
Bse3DI GCAATG 1 cut(s) 331
Bse8I GATNNNNATC 1 cut(s) 737
BseDI CCNNGG 2 cut(s) 220, 420
BseGI GGATG 3 cut(s) 137, 577, 817
BseJI GATNNNNATC 1 cut(s) 737
BseLI CCNNNNNNNGG 3 cut(s) 237, 892, 1007
BseMI GCAATG 1 cut(s) 331
BseMII CTCAG 4 cut(s) 199, 224, 540, 854
BseNI ACTGG 2 cut(s) 309, 427
BseXI GCAGC 3 cut(s) 502, 763, 769
BsgI GTGCAG 1 cut(s) 770
BshFI GGCC 4 cut(s) 468, 845, 885, 896
BsiHKCI CYCGRG 1 cut(s) 365
BsiSI CCGG 3 cut(s) 231, 761, 977
BslFI GGGAC 1 cut(s) 786
BslI CCNNNNNNNGG 3 cut(s) 237, 892, 1007
BsmAI GTCTC 4 cut(s) 213, 467, 927, 1022
BsmFI GGGAC 1 cut(s) 786
BsmI GAATGC 2 cut(s) 225, 576
BsnI GGCC 4 cut(s) 468, 845, 885, 896
Bso31I GGTCTC 1 cut(s) 467
BsoBI CYCGRG 1 cut(s) 365
Bsp143I GATC 5 cut(s) 41, 94, 134, 346, 732
BspACI CCGC 1 cut(s) 480
BspANI GGCC 4 cut(s) 468, 845, 885, 896
BspCNI CTCAG 4 cut(s) 198, 223, 541, 853
BspLI GGNNCC 2 cut(s) 721, 774
BspPI GGATC 2 cut(s) 354, 727
BspTI CTTAAG 1 cut(s) 235
BspTNI GGTCTC 1 cut(s) 467
BsrDI GCAATG 1 cut(s) 331
BsrI ACTGG 2 cut(s) 309, 427
BssECI CCNNGG 2 cut(s) 220, 420
BssMI GATC 5 cut(s) 41, 94, 134, 346, 732
BssSI CACGAG 1 cut(s) 537
BssT1I CCWWGG 2 cut(s) 220, 420
Bst2BI CACGAG 1 cut(s) 537
Bst6I CTCTTC 1 cut(s) 198
BstAFI CTTAAG 1 cut(s) 235
BstAPI GCANNNNNTGC 1 cut(s) 757
BstC8I GCNNGC 5 cut(s) 466, 503, 534, 829, 871
BstDEI CTNAG 4 cut(s) 185, 210, 549, 840
BstF5I GGATG 3 cut(s) 137, 577, 817
BstH2I RGCGCY 1 cut(s) 870
BstHHI GCGC 3 cut(s) 324, 642, 869
BstKTI GATC 5 cut(s) 44, 97, 137, 349, 735
BstMAI GTCTC 4 cut(s) 213, 467, 927, 1022
BstMBI GATC 5 cut(s) 41, 94, 134, 346, 732
BstMWI GCNNNNNNNGC 4 cut(s) 231, 488, 573, 757
BstNSI RCATGY 1 cut(s) 677
BstSCI CCNGG 2 cut(s) 229, 975
BstSFI CTRYAG 1 cut(s) 24
BstV1I GCAGC 3 cut(s) 502, 763, 769
BstX2I RGATCY 3 cut(s) 41, 346, 732
BstYI RGATCY 3 cut(s) 41, 346, 732
Bsu36I CCTNAGG 1 cut(s) 840
BsuRI GGCC 4 cut(s) 468, 845, 885, 896
BtsCI GGATG 3 cut(s) 137, 577, 817
BtsI GCAGTG 1 cut(s) 635
BtsIMutI CAGTG 1 cut(s) 635
Cac8I GCNNGC 5 cut(s) 466, 503, 534, 829, 871
CfoI GCGC 3 cut(s) 324, 642, 869
Cfr13I GGNCC 2 cut(s) 424, 773
CseI GACGC 1 cut(s) 18
Csp6I GTAC 1 cut(s) 646
CviAII CATG 7 cut(s) 470, 495, 604, 674, 898, 1007, 1022
CviQI GTAC 1 cut(s) 646
DdeI CTNAG 4 cut(s) 185, 210, 549, 840
DpnI GATC 5 cut(s) 43, 96, 136, 348, 734
DpnII GATC 5 cut(s) 41, 94, 134, 346, 732
EaeI YGGCCR 3 cut(s) 466, 883, 894
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco130I CCWWGG 2 cut(s) 220, 420
Eco147I AGGCCT 1 cut(s) 845
Eco31I GGTCTC 1 cut(s) 467
Eco47I GGWCC 2 cut(s) 424, 773
Eco81I CCTNAGG 1 cut(s) 840
Eco88I CYCGRG 1 cut(s) 365
EcoO109I RGGNCCY 1 cut(s) 773
EcoRI GAATTC 1 cut(s) 816
EcoT14I CCWWGG 2 cut(s) 220, 420
EcoT22I ATGCAT 1 cut(s) 679
ErhI CCWWGG 2 cut(s) 220, 420
FaeI CATG 7 cut(s) 473, 498, 607, 677, 901, 1010, 1025
FaqI GGGAC 1 cut(s) 786
FatI CATG 7 cut(s) 469, 494, 603, 673, 897, 1006, 1021
FbaI TGATCA 1 cut(s) 94
Fnu4HI GCNGC 3 cut(s) 516, 752, 783
FokI GGATG 3 cut(s) 124, 584, 824
Fsp4HI GCNGC 3 cut(s) 516, 752, 783
FspBI CTAG 1 cut(s) 278
GlaI GCGC 3 cut(s) 323, 641, 868
GluI GCNGC 3 cut(s) 516, 752, 783
GsuI CTGGAG 1 cut(s) 326
HaeII RGCGCY 1 cut(s) 870
HaeIII GGCC 4 cut(s) 468, 845, 885, 896
HapII CCGG 3 cut(s) 231, 761, 977
HgaI GACGC 1 cut(s) 18
HhaI GCGC 3 cut(s) 324, 642, 869
Hin1II CATG 7 cut(s) 473, 498, 607, 677, 901, 1010, 1025
Hin6I GCGC 3 cut(s) 322, 640, 867
HinP1I GCGC 3 cut(s) 322, 640, 867
HindIII AAGCTT 1 cut(s) 109
HinfI GANTC 7 cut(s) 20, 190, 274, 289, 625, 804, 916
HpaII CCGG 3 cut(s) 231, 761, 977
HphI GGTGA 3 cut(s) 634, 642, 925
Hpy166II GTNNAC 1 cut(s) 19
Hpy188I TCNGA 2 cut(s) 388, 732
Hpy188III TCNNGA 6 cut(s) 98, 132, 187, 278, 524, 629
Hpy8I GTNNAC 1 cut(s) 19
HpyAV CCTTC 2 cut(s) 337, 904
HpyCH4V TGCA 7 cut(s) 447, 518, 567, 677, 751, 831, 962
HpyF10VI GCNNNNNNNGC 4 cut(s) 231, 488, 573, 757
HpyF3I CTNAG 4 cut(s) 185, 210, 549, 840
Hsp92II CATG 7 cut(s) 473, 498, 607, 677, 901, 1010, 1025
HspAI GCGC 3 cut(s) 322, 640, 867
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 5 cut(s) 41, 94, 134, 346, 732
LmnI GCTCC 1 cut(s) 371
Lsp1109I GCAGC 3 cut(s) 502, 763, 769
LweI GCATC 3 cut(s) 379, 500, 769
MaeI CTAG 1 cut(s) 278
MaeIII GTNAC 1 cut(s) 30
MalI GATC 5 cut(s) 43, 96, 136, 348, 734
MboI GATC 5 cut(s) 41, 94, 134, 346, 732
MboII GAAGA 4 cut(s) 185, 341, 344, 695
MfeI CAATTG 1 cut(s) 755
MflI RGATCY 3 cut(s) 41, 346, 732
MlsI TGGCCA 3 cut(s) 468, 885, 896
MluCI AATT 9 cut(s) 151, 379, 442, 561, 708, 755, 764, 816, 905
MluNI TGGCCA 3 cut(s) 468, 885, 896
MlyI GAGTC 3 cut(s) 14, 184, 283
Mox20I TGGCCA 3 cut(s) 468, 885, 896
Mph1103I ATGCAT 1 cut(s) 679
MscI TGGCCA 3 cut(s) 468, 885, 896
MseI TTAA 6 cut(s) 11, 38, 236, 690, 696, 792
MslI CAYNNNNRTG 1 cut(s) 496
Msp20I TGGCCA 3 cut(s) 468, 885, 896
MspCI CTTAAG 1 cut(s) 235
MspI CCGG 3 cut(s) 231, 761, 977
MspR9I CCNGG 2 cut(s) 231, 977
MunI CAATTG 1 cut(s) 755
Mva1269I GAATGC 2 cut(s) 225, 576
MwoI GCNNNNNNNGC 4 cut(s) 231, 488, 573, 757
NciI CCSGG 2 cut(s) 231, 977
NdeII GATC 5 cut(s) 41, 94, 134, 346, 732
NlaIII CATG 7 cut(s) 473, 498, 607, 677, 901, 1010, 1025
NlaIV GGNNCC 2 cut(s) 721, 774
NmuCI GTSAC 1 cut(s) 30
NsiI ATGCAT 1 cut(s) 679
NspI RCATGY 1 cut(s) 677
PaeR7I CTCGAG 1 cut(s) 365
PceI AGGCCT 1 cut(s) 845
PctI GAATGC 2 cut(s) 225, 576
PfeI GAWTC 4 cut(s) 289, 625, 804, 916
PflMI CCANNNNNTGG 1 cut(s) 892
PkrI GCNGC 3 cut(s) 517, 753, 784
PleI GAGTC 3 cut(s) 14, 184, 282
PpsI GAGTC 3 cut(s) 14, 184, 282
PpuMI RGGWCCY 1 cut(s) 773
Psp5II RGGWCCY 1 cut(s) 773
PspN4I GGNNCC 2 cut(s) 721, 774
PspPI GGNCC 2 cut(s) 424, 773
PspPPI RGGWCCY 1 cut(s) 773
PspXI VCTCGAGB 1 cut(s) 365
PsuI RGATCY 3 cut(s) 41, 346, 732
RsaI GTAC 1 cut(s) 647
RsaNI GTAC 1 cut(s) 646
RseI CAYNNNNRTG 1 cut(s) 496
SaqAI TTAA 6 cut(s) 11, 38, 236, 690, 696, 792
SatI GCNGC 3 cut(s) 516, 752, 783
Sau3AI GATC 5 cut(s) 41, 94, 134, 346, 732
Sau96I GGNCC 2 cut(s) 424, 773
SchI GAGTC 3 cut(s) 14, 184, 283
ScrFI CCNGG 2 cut(s) 231, 977
SfaNI GCATC 3 cut(s) 379, 500, 769
SfcI CTRYAG 1 cut(s) 24
Sfr274I CTCGAG 1 cut(s) 365
SinI GGWCC 2 cut(s) 424, 773
SlaI CTCGAG 1 cut(s) 365
SmiMI CAYNNNNRTG 1 cut(s) 496
SmlI CTYRAG 2 cut(s) 235, 365
SmoI CTYRAG 2 cut(s) 235, 365
Sse9I AATT 9 cut(s) 151, 379, 442, 561, 708, 755, 764, 816, 905
SseBI AGGCCT 1 cut(s) 845
SsiI CCGC 1 cut(s) 480
SspMI CTAG 1 cut(s) 278
StuI AGGCCT 1 cut(s) 845
StyD4I CCNGG 2 cut(s) 229, 975
StyI CCWWGG 2 cut(s) 220, 420
TaqI TCGA 5 cut(s) 6, 366, 598, 807, 954
TasI AATT 9 cut(s) 151, 379, 442, 561, 708, 755, 764, 816, 905
TatI WGTACW 1 cut(s) 645
TfiI GAWTC 4 cut(s) 289, 625, 804, 916
Tru1I TTAA 6 cut(s) 11, 38, 236, 690, 696, 792
Tru9I TTAA 6 cut(s) 11, 38, 236, 690, 696, 792
TscAI CASTG 1 cut(s) 642
TseFI GTSAC 1 cut(s) 30
TseI GCWGC 3 cut(s) 515, 751, 782
Tsp45I GTSAC 1 cut(s) 30
TspDTI ATGAA 4 cut(s) 396, 421, 620, 652
TspRI CASTG 1 cut(s) 642
Van91I CCANNNNNTGG 1 cut(s) 892
Vha464I CTTAAG 1 cut(s) 235
VpaK11BI GGWCC 2 cut(s) 424, 773
XapI RAATTY 2 cut(s) 561, 816
XbaI TCTAGA 1 cut(s) 277
XceI RCATGY 1 cut(s) 677
XhoI CTCGAG 1 cut(s) 365
XspI CTAG 1 cut(s) 278
Zsp2I ATGCAT 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.