Rh1DG190600

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005196
Physical Location & Seq
Forward (+)
38025478 .. 38029876
4399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG190600.1

Sequence Viewer

Length: 1164 bp
ATGAAGAAGATCAAAATCGAGGCGATGAAGAAGCCAGCAGCTACAACAACAGAAGCAAAGGATCACCCACTTTCTATTGCTCATGATTGGCCTTCAAATCTATTCCCTCATATTGCGCAGTGGCGAAAGGAATATGGGCCAATCTTCACCTATTCAACTGGAAGCATACAGCTATTAAGCGTTACAGAGATAGAGATGGTGAAGGAAATTAGCATGTGCACATCTTTGATTTTAGGAAGGCCTGCTTATATGTCGAAGGATTTGAAGCCCCTATTAGGACAAGGCATTATCTCTTCAAGTGGCCAAACTTGGTCACACCAGAGGAAAATCATTTCTCCTGAATTCTACTTTGATAAGGTTAAGGATATGGTCGATTTAATGGTGGACTCTACAGCGTCACTATTAAGATCTTGGGAAAGCAAAGTTGAAAGTGAGGGAGGAATGGCATTGTTTAGGATTGATCAAGATTTGAGAAGCTTGTCAGCAGATATAATATCAAGATCATCCTTTGGAAGTAATTATTCAAAGGGGAAAGAAATCTTTTCAAAGCTCAGGACTCTTCAACAAGTTATGTCTCAGGGAAACCTTGGCATTCCCGGCTTAAGGCACTTGCCAACAAAGAGGAACAGAGAGATAAGGAGTCTAGAGAAAGAGATTCACTCAATGATACTGGAGGTTGCCAAAAAGCGCAATGAGGCTACTTATGAGAAGGATCTTCTTCAAATGATACTCGAGGGAGCAAAAAATTACTCTGATGCTGATAGCTTTTTTTCATCTGGTATGTCCAAGGACCAGTTCATAGTGGATAATTGCAAGAATATATATTTTGCTGGCCATGAGACCACCGCCATCACAGCATCATGGTGCTTGCTGTTGTTGGCTGCATATCCAGAGTGGCAAGCTCGTGTTCGTGCTGAGGTGCTTGAAATTTGCAAGGATGGCATTCCAAATGCTGATGTGCTTCGAGGCATGAAAACATTGTTGTATGACAAGAGCAAGGAACAAAGAGGTCGCCAGAAGAGTGAATTCAAGAAGGAATCTGTTGATCATCATCATTATAAATTGGTGAACACAGGAGATGAACAACACCCCCAACACGGTGATCGCTACAATTTCCACAAAAACACGACCATTGCCGTCATTGAGTTCGTCCAGGCGAGTTAG

Protein Analysis

387

Amino Acids

44.03

Weight (kDa)

8.77

Isoelectric Point (pI)

45.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 34 - 314 3.1e-36 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1059
Acc16I TGCGCA 1 cut(s) 117
AciI CCGC 1 cut(s) 846
AclWI GGATC 2 cut(s) 69, 720
AcoI YGGCCR 2 cut(s) 301, 832
AcsI RAATTY 3 cut(s) 341, 927, 1025
AfiI CCNNNNNNNGG 3 cut(s) 275, 603, 1097
AflII CTTAAG 1 cut(s) 601
AjnI CCWGG 1 cut(s) 1152
AjuI GAANNNNNNNTTGG 2 cut(s) 779, 811
AluBI AGCT 6 cut(s) 41, 172, 477, 550, 765, 902
AluI AGCT 6 cut(s) 41, 172, 477, 550, 765, 902
Alw21I GWGCWC 1 cut(s) 221
Alw26I GTCTC 2 cut(s) 579, 833
Alw44I GTGCAC 1 cut(s) 217
AlwI GGATC 2 cut(s) 69, 720
Ama87I CYCGRG 1 cut(s) 731
AoxI GGCC 5 cut(s) 89, 137, 239, 301, 832
ApaLI GTGCAC 1 cut(s) 217
ApeKI GCWGC 2 cut(s) 38, 881
ApoI RAATTY 3 cut(s) 341, 927, 1025
AspLEI GCGC 2 cut(s) 118, 690
AspS9I GGNCC 2 cut(s) 137, 790
AsuC2I CCSGG 1 cut(s) 597
AsuHPI GGTGA 5 cut(s) 56, 139, 211, 1078, 1112
AvaI CYCGRG 1 cut(s) 731
AvaII GGWCC 1 cut(s) 790
BaeGI GKGCMC 1 cut(s) 221
BalI TGGCCA 2 cut(s) 303, 834
BauI CACGAG 1 cut(s) 903
Bbv12I GWGCWC 1 cut(s) 221
BbvCI CCTCAGC 1 cut(s) 915
BbvI GCAGC 2 cut(s) 50, 868
BccI CCATC 3 cut(s) 190, 857, 932
BceAI ACGGC 1 cut(s) 1121
BciT130I CCWGG 1 cut(s) 1154
BclI TGATCA 2 cut(s) 460, 1045
BcnI CCSGG 1 cut(s) 597
BcoDI GTCTC 2 cut(s) 579, 833
BfaI CTAG 1 cut(s) 644
BfmI CTRYAG 1 cut(s) 390
BfrI CTTAAG 1 cut(s) 601
BglII AGATCT 1 cut(s) 407
BisI GCNGC 2 cut(s) 39, 882
BlsI GCNGC 2 cut(s) 40, 883
Bme1390I CCNGG 2 cut(s) 597, 1154
Bme18I GGWCC 1 cut(s) 790
BmeT110I CYCGRG 1 cut(s) 731
BmgT120I GGNCC 2 cut(s) 137, 790
BmrFI CCNGG 2 cut(s) 597, 1154
BmsI GCATC 2 cut(s) 745, 866
BplI GAGNNNNNCTC 2 cut(s) 644, 676
BpmI CTGGAG 1 cut(s) 692
Bpu10I CCTNAGC 2 cut(s) 551, 915
BpuMI CCSGG 1 cut(s) 597
BsaBI GATNNNNATC 2 cut(s) 14, 1050
BsaI GGTCTC 1 cut(s) 833
BsaJI CCNNGG 2 cut(s) 586, 786
Bsc4I CCNNNNNNNGG 3 cut(s) 275, 603, 1097
Bse1I ACTGG 3 cut(s) 163, 675, 793
Bse3DI GCAATG 2 cut(s) 697, 1131
Bse8I GATNNNNATC 2 cut(s) 14, 1050
BseBI CCWGG 1 cut(s) 1154
BseDI CCNNGG 2 cut(s) 586, 786
BseGI GGATG 2 cut(s) 503, 943
BseJI GATNNNNATC 2 cut(s) 14, 1050
BseLI CCNNNNNNNGG 3 cut(s) 275, 603, 1097
BseMI GCAATG 2 cut(s) 697, 1131
BseMII CTCAG 3 cut(s) 565, 590, 906
BseNI ACTGG 3 cut(s) 163, 675, 793
BseSI GKGCMC 1 cut(s) 221
BseXI GCAGC 2 cut(s) 50, 868
BshFI GGCC 5 cut(s) 91, 139, 241, 303, 834
BsiHKAI GWGCWC 1 cut(s) 221
BsiHKCI CYCGRG 1 cut(s) 731
BsiSI CCGG 1 cut(s) 597
BslI CCNNNNNNNGG 3 cut(s) 275, 603, 1097
BsmAI GTCTC 2 cut(s) 579, 833
BsmI GAATGC 2 cut(s) 591, 942
BsnI GGCC 5 cut(s) 91, 139, 241, 303, 834
Bso31I GGTCTC 1 cut(s) 833
BsoBI CYCGRG 1 cut(s) 731
Bsp1286I GDGCHC 1 cut(s) 221
Bsp143I GATC 8 cut(s) 9, 61, 407, 460, 500, 712, 1045, 1102
BspACI CCGC 1 cut(s) 846
BspANI GGCC 5 cut(s) 91, 139, 241, 303, 834
BspCNI CTCAG 3 cut(s) 564, 589, 907
BspHI TCATGA 1 cut(s) 82
BspPI GGATC 2 cut(s) 69, 720
BspTI CTTAAG 1 cut(s) 601
BspTNI GGTCTC 1 cut(s) 833
BsrDI GCAATG 2 cut(s) 697, 1131
BsrI ACTGG 3 cut(s) 163, 675, 793
BssECI CCNNGG 2 cut(s) 586, 786
BssMI GATC 8 cut(s) 9, 61, 407, 460, 500, 712, 1045, 1102
BssSI CACGAG 1 cut(s) 903
BssT1I CCWWGG 2 cut(s) 586, 786
Bst2BI CACGAG 1 cut(s) 903
Bst2UI CCWGG 1 cut(s) 1154
Bst4CI ACNGT 1 cut(s) 1100
Bst6I CTCTTC 3 cut(s) 298, 564, 1013
BstAFI CTTAAG 1 cut(s) 601
BstC8I GCNNGC 5 cut(s) 36, 243, 832, 869, 900
BstDEI CTNAG 3 cut(s) 551, 576, 915
BstF5I GGATG 2 cut(s) 503, 943
BstHHI GCGC 2 cut(s) 118, 690
BstKTI GATC 8 cut(s) 12, 64, 410, 463, 503, 715, 1048, 1105
BstMAI GTCTC 2 cut(s) 579, 833
BstMBI GATC 8 cut(s) 9, 61, 407, 460, 500, 712, 1045, 1102
BstMWI GCNNNNNNNGC 3 cut(s) 597, 854, 939
BstNI CCWGG 1 cut(s) 1154
BstNSI RCATGY 1 cut(s) 217
BstSCI CCNGG 2 cut(s) 595, 1152
BstSFI CTRYAG 1 cut(s) 390
BstSLI GKGCMC 1 cut(s) 221
BstV1I GCAGC 2 cut(s) 50, 868
BstX2I RGATCY 2 cut(s) 407, 712
BstYI RGATCY 2 cut(s) 407, 712
BsuRI GGCC 5 cut(s) 91, 139, 241, 303, 834
BtgZI GCGATG 1 cut(s) 38
BtsCI GGATG 2 cut(s) 503, 943
BtsI GCAGTG 1 cut(s) 125
BtsIMutI CAGTG 1 cut(s) 125
Cac8I GCNNGC 5 cut(s) 36, 243, 832, 869, 900
CciI TCATGA 1 cut(s) 82
CfoI GCGC 2 cut(s) 118, 690
Cfr13I GGNCC 2 cut(s) 137, 790
CseI GACGC 1 cut(s) 384
CviAII CATG 5 cut(s) 83, 214, 836, 861, 970
DdeI CTNAG 3 cut(s) 551, 576, 915
DpnI GATC 8 cut(s) 11, 63, 409, 462, 502, 714, 1047, 1104
DpnII GATC 8 cut(s) 9, 61, 407, 460, 500, 712, 1045, 1102
EaeI YGGCCR 2 cut(s) 301, 832
Eam1104I CTCTTC 3 cut(s) 298, 564, 1013
EarI CTCTTC 3 cut(s) 298, 564, 1013
Eco130I CCWWGG 2 cut(s) 586, 786
Eco147I AGGCCT 1 cut(s) 241
Eco31I GGTCTC 1 cut(s) 833
Eco47I GGWCC 1 cut(s) 790
Eco88I CYCGRG 1 cut(s) 731
EcoRI GAATTC 2 cut(s) 341, 1025
EcoRII CCWGG 1 cut(s) 1152
EcoT14I CCWWGG 2 cut(s) 586, 786
ErhI CCWWGG 2 cut(s) 586, 786
FaeI CATG 5 cut(s) 86, 217, 839, 864, 973
FalI AAGNNNNNCTT 2 cut(s) 229, 261
FatI CATG 5 cut(s) 82, 213, 835, 860, 969
FbaI TGATCA 2 cut(s) 460, 1045
Fnu4HI GCNGC 2 cut(s) 39, 882
FokI GGATG 2 cut(s) 490, 950
Fsp4HI GCNGC 2 cut(s) 39, 882
FspBI CTAG 1 cut(s) 644
FspI TGCGCA 1 cut(s) 117
GlaI GCGC 2 cut(s) 117, 689
GluI GCNGC 2 cut(s) 39, 882
GsuI CTGGAG 1 cut(s) 692
HaeIII GGCC 5 cut(s) 91, 139, 241, 303, 834
HapII CCGG 1 cut(s) 597
HgaI GACGC 1 cut(s) 384
HhaI GCGC 2 cut(s) 118, 690
Hin1II CATG 5 cut(s) 86, 217, 839, 864, 973
Hin6I GCGC 2 cut(s) 116, 688
HinP1I GCGC 2 cut(s) 116, 688
HindIII AAGCTT 1 cut(s) 475
HinfI GANTC 5 cut(s) 386, 556, 640, 655, 1037
HpaII CCGG 1 cut(s) 597
HphI GGTGA 5 cut(s) 56, 139, 211, 1078, 1112
Hpy166II GTNNAC 3 cut(s) 219, 385, 1069
Hpy188I TCNGA 1 cut(s) 754
Hpy188III TCNNGA 8 cut(s) 83, 338, 464, 498, 553, 644, 890, 1030
Hpy8I GTNNAC 3 cut(s) 219, 385, 1069
HpyAV CCTTC 6 cut(s) 102, 196, 231, 250, 703, 1027
HpyCH4III ACNGT 1 cut(s) 1100
HpyCH4V TGCA 4 cut(s) 219, 813, 884, 933
HpyF10VI GCNNNNNNNGC 3 cut(s) 597, 854, 939
HpyF3I CTNAG 3 cut(s) 551, 576, 915
Hsp92II CATG 5 cut(s) 86, 217, 839, 864, 973
HspAI GCGC 2 cut(s) 116, 688
Ksp22I TGATCA 2 cut(s) 460, 1045
Kzo9I GATC 8 cut(s) 9, 61, 407, 460, 500, 712, 1045, 1102
LmnI GCTCC 1 cut(s) 737
Lsp1109I GCAGC 2 cut(s) 50, 868
LweI GCATC 2 cut(s) 745, 866
MaeI CTAG 1 cut(s) 644
MaeIII GTNAC 3 cut(s) 181, 312, 396
MalI GATC 8 cut(s) 11, 63, 409, 462, 502, 714, 1047, 1104
MboI GATC 8 cut(s) 9, 61, 407, 460, 500, 712, 1045, 1102
MboII GAAGA 9 cut(s) 16, 19, 40, 136, 285, 551, 707, 710, 1030
MflI RGATCY 2 cut(s) 407, 712
MhlI GDGCHC 1 cut(s) 221
MlsI TGGCCA 2 cut(s) 303, 834
MluCI AATT 9 cut(s) 207, 341, 517, 745, 808, 927, 1025, 1061, 1111
MluNI TGGCCA 2 cut(s) 303, 834
MlyI GAGTC 3 cut(s) 380, 550, 649
Mox20I TGGCCA 2 cut(s) 303, 834
MscI TGGCCA 2 cut(s) 303, 834
MseI TTAA 5 cut(s) 176, 360, 377, 404, 602
MslI CAYNNNNRTG 1 cut(s) 862
Msp20I TGGCCA 2 cut(s) 303, 834
MspCI CTTAAG 1 cut(s) 601
MspI CCGG 1 cut(s) 597
MspR9I CCNGG 2 cut(s) 597, 1154
Mva1269I GAATGC 2 cut(s) 591, 942
MvaI CCWGG 1 cut(s) 1154
MwoI GCNNNNNNNGC 3 cut(s) 597, 854, 939
NciI CCSGG 1 cut(s) 597
NdeII GATC 8 cut(s) 9, 61, 407, 460, 500, 712, 1045, 1102
NlaIII CATG 5 cut(s) 86, 217, 839, 864, 973
NmuCI GTSAC 2 cut(s) 312, 396
NsbI TGCGCA 1 cut(s) 117
NspI RCATGY 1 cut(s) 217
PaeR7I CTCGAG 1 cut(s) 731
PagI TCATGA 1 cut(s) 82
PceI AGGCCT 1 cut(s) 241
PctI GAATGC 2 cut(s) 591, 942
PfeI GAWTC 2 cut(s) 655, 1037
PkrI GCNGC 2 cut(s) 40, 883
PleI GAGTC 3 cut(s) 380, 550, 648
PpsI GAGTC 3 cut(s) 380, 550, 648
PsiI TTATAA 1 cut(s) 1059
Psp6I CCWGG 1 cut(s) 1152
PspGI CCWGG 1 cut(s) 1152
PspPI GGNCC 2 cut(s) 137, 790
PspXI VCTCGAGB 1 cut(s) 731
PsuI RGATCY 2 cut(s) 407, 712
RseI CAYNNNNRTG 1 cut(s) 862
SaqAI TTAA 5 cut(s) 176, 360, 377, 404, 602
SatI GCNGC 2 cut(s) 39, 882
Sau3AI GATC 8 cut(s) 9, 61, 407, 460, 500, 712, 1045, 1102
Sau96I GGNCC 2 cut(s) 137, 790
SchI GAGTC 3 cut(s) 380, 550, 649
ScrFI CCNGG 2 cut(s) 597, 1154
SduI GDGCHC 1 cut(s) 221
SfaNI GCATC 2 cut(s) 745, 866
SfcI CTRYAG 1 cut(s) 390
Sfr274I CTCGAG 1 cut(s) 731
SinI GGWCC 1 cut(s) 790
SlaI CTCGAG 1 cut(s) 731
SmiMI CAYNNNNRTG 1 cut(s) 862
SmlI CTYRAG 2 cut(s) 601, 731
SmoI CTYRAG 2 cut(s) 601, 731
Sse9I AATT 9 cut(s) 207, 341, 517, 745, 808, 927, 1025, 1061, 1111
SseBI AGGCCT 1 cut(s) 241
SsiI CCGC 1 cut(s) 846
SspMI CTAG 1 cut(s) 644
StuI AGGCCT 1 cut(s) 241
StyD4I CCNGG 2 cut(s) 595, 1152
StyI CCWWGG 2 cut(s) 586, 786
TaaI ACNGT 1 cut(s) 1100
TaqI TCGA 5 cut(s) 18, 254, 372, 732, 964
TasI AATT 9 cut(s) 207, 341, 517, 745, 808, 927, 1025, 1061, 1111
TfiI GAWTC 2 cut(s) 655, 1037
Tru1I TTAA 5 cut(s) 176, 360, 377, 404, 602
Tru9I TTAA 5 cut(s) 176, 360, 377, 404, 602
TscAI CASTG 1 cut(s) 125
TseFI GTSAC 2 cut(s) 312, 396
TseI GCWGC 2 cut(s) 38, 881
Tsp45I GTSAC 2 cut(s) 312, 396
TspDTI ATGAA 6 cut(s) 17, 41, 762, 787, 986, 1095
TspRI CASTG 1 cut(s) 125
Vha464I CTTAAG 1 cut(s) 601
VneI GTGCAC 1 cut(s) 217
VpaK11BI GGWCC 1 cut(s) 790
XapI RAATTY 3 cut(s) 341, 927, 1025
XbaI TCTAGA 1 cut(s) 643
XceI RCATGY 1 cut(s) 217
XhoI CTCGAG 1 cut(s) 731
XspI CTAG 1 cut(s) 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.