Rh1AG193300

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0005196
Physical Location & Seq
Forward (+)
36884371 .. 36888664
4294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG193300.1

Sequence Viewer

Length: 1293 bp
ATGTTGATGAGTGATGTGATGACAGGGCCAATCTTCACCTATTCAACTGGAAGCATACAGCTATTAAGCGTTACAGAGATAGAGATGGTGAAGGAAATTAGCATGTGCACATCTTTGATTTTAGGAAGGCCTGCTTATATGTCTAAGGATTTGAAGCCCCTATTAGGACAAGGCATTATCTCTTCAAGTGGCCAAACTTGGTCACACCAGAGGAAAATCATTTCTCCTGAATTCTACTTTGATAAGGTTAAGGATATGGTCGATTTAATGGTGGACTCTACAGCGTCACTATTAAGATCTTGGGAAAGCAAAGTTGAAAGTGAGGGAGGAATGGCATTGTTTAGGATTGATCAAGATTTGAGAAGCTTGTCAGCGGATATAATATCAAGATCATCCTTTGGAAGTAATTATTCAAAGGGGAAAGAAATCTTTTCAAAGCTCAGGACTCTTCAACAAGTTATGTCTCAAGGAAACCTTGGCATTCCCGGCGTAAGGCACTTGCCAACAAAGAGGAACAGAGAGATAAGGAGTCTAGAGAACGAGATTCACTCAATGATACTGGAGGTTGCAAAAAAGCGCAATGAGGCTACTTATGAGAAGGATCTTCTTCAAATGATACTCGAGGGAGCAAAAAATTACTCTGATGCTGATAGCTTTTTTTCATCTGGTATGTCTGAGGACCAGTTCATAGTGGATAATTGCAAGAATATATATTTTGCTGGCCATGAGACCACCGCCATCACAGCATCATGGTGCTTGCTGTTGTTGGCTGCATATCCAGAGTGGCAAGCTCGTGTTCGTGCTGAGGTGCTTGAAATTTGCAAGGATGGCATTCCAAATGCTGATGTGCTTCGAGGCATGAAAACATTGACTATGGTGATTCAAGAAACACTGCGCTTGTACTCACCAGCAACTTTCATCGTAAGACATGCATTGGAAGATATTAAACTCAAAGATATTCTAATTCCCAAAGGAACCACCATTCAGATCCCAATCTCAATGGTGCAGCAATTGCCGGAATTATGGGGACCTGATGCTGCTGACTTTAATCCCAATAGATTCGAGGATGGAATTCGTGGGGCTTGCAAGTTTCCTCAGGCCTATTTCCCATTTGGATTTGGCGCTCGCATTTGTGTTGGCCAAAACTTGGCCATGACAGAATTGAAGGTGATTCTGTCTCTCATATTATCCAAGTTATCATTCTCGCTCGACCCTGCATACCAACACAGCCCGGTTTTTAGGTTGGTTATTGAACCTGAACATGGAGTAAGTCTCCATGTGAGGAGGGTGTGA

Protein Analysis

430

Amino Acids

48.29

Weight (kDa)

6.48

Isoelectric Point (pI)

49.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 8 - 414 9.6e-76 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1147
AciI CCGC 2 cut(s) 374, 735
AclWI GGATC 2 cut(s) 609, 982
AcoI YGGCCR 4 cut(s) 190, 721, 1138, 1149
AcsI RAATTY 3 cut(s) 230, 816, 1071
AfaI GTAC 1 cut(s) 902
AfiI CCNNNNNNNGG 4 cut(s) 164, 492, 1147, 1262
AjuI GAANNNNNNNTTGG 2 cut(s) 1184, 1216
AluBI AGCT 5 cut(s) 61, 366, 439, 654, 791
AluI AGCT 5 cut(s) 61, 366, 439, 654, 791
Alw21I GWGCWC 1 cut(s) 110
Alw26I GTCTC 4 cut(s) 468, 722, 1182, 1277
Alw44I GTGCAC 1 cut(s) 106
AlwI GGATC 2 cut(s) 609, 982
Ama87I CYCGRG 1 cut(s) 620
AoxI GGCC 7 cut(s) 26, 128, 190, 721, 1098, 1138, 1149
ApaLI GTGCAC 1 cut(s) 106
ApeKI GCWGC 3 cut(s) 770, 1006, 1037
ApoI RAATTY 3 cut(s) 230, 816, 1071
AspLEI GCGC 3 cut(s) 579, 897, 1124
AspS9I GGNCC 3 cut(s) 26, 679, 1028
AsuC2I CCSGG 2 cut(s) 486, 1232
AsuHPI GGTGA 5 cut(s) 28, 100, 889, 897, 1180
AvaI CYCGRG 1 cut(s) 620
AvaII GGWCC 2 cut(s) 679, 1028
AxyI CCTNAGG 1 cut(s) 1095
BaeGI GKGCMC 1 cut(s) 110
BalI TGGCCA 4 cut(s) 192, 723, 1140, 1151
BauI CACGAG 1 cut(s) 792
Bbv12I GWGCWC 1 cut(s) 110
BbvCI CCTCAGC 1 cut(s) 804
BbvI GCAGC 3 cut(s) 757, 1018, 1024
BccI CCATC 4 cut(s) 79, 746, 821, 1061
BclI TGATCA 1 cut(s) 349
BcnI CCSGG 2 cut(s) 486, 1232
BcoDI GTCTC 4 cut(s) 468, 722, 1182, 1277
BfaI CTAG 1 cut(s) 533
BfmI CTRYAG 1 cut(s) 279
BfoI RGCGCY 1 cut(s) 1125
BglII AGATCT 1 cut(s) 296
BisI GCNGC 3 cut(s) 771, 1007, 1038
BlsI GCNGC 3 cut(s) 772, 1008, 1039
Bme1390I CCNGG 2 cut(s) 486, 1232
Bme18I GGWCC 2 cut(s) 679, 1028
BmeT110I CYCGRG 1 cut(s) 620
BmgT120I GGNCC 3 cut(s) 26, 679, 1028
BmiI GGNNCC 2 cut(s) 976, 1029
BmrFI CCNGG 2 cut(s) 486, 1232
BmsI GCATC 3 cut(s) 634, 755, 1024
BplI GAGNNNNNCTC 4 cut(s) 533, 565, 1257, 1289
BpmI CTGGAG 1 cut(s) 581
Bpu10I CCTNAGC 2 cut(s) 440, 804
BpuEI CTTGAG 1 cut(s) 450
BpuMI CCSGG 2 cut(s) 486, 1232
BsaBI GATNNNNATC 1 cut(s) 992
BsaI GGTCTC 1 cut(s) 722
BsaJI CCNNGG 1 cut(s) 475
Bsc4I CCNNNNNNNGG 4 cut(s) 164, 492, 1147, 1262
Bse1I ACTGG 3 cut(s) 52, 564, 682
Bse21I CCTNAGG 1 cut(s) 1095
Bse3DI GCAATG 1 cut(s) 586
Bse8I GATNNNNATC 1 cut(s) 992
BseDI CCNNGG 1 cut(s) 475
BseGI GGATG 3 cut(s) 392, 832, 1072
BseJI GATNNNNATC 1 cut(s) 992
BseLI CCNNNNNNNGG 4 cut(s) 164, 492, 1147, 1262
BseMI GCAATG 1 cut(s) 586
BseMII CTCAG 4 cut(s) 454, 666, 795, 1109
BseNI ACTGG 3 cut(s) 52, 564, 682
BseSI GKGCMC 1 cut(s) 110
BseXI GCAGC 3 cut(s) 757, 1018, 1024
BsgI GTGCAG 1 cut(s) 1025
BshFI GGCC 7 cut(s) 28, 130, 192, 723, 1100, 1140, 1151
BsiHKAI GWGCWC 1 cut(s) 110
BsiHKCI CYCGRG 1 cut(s) 620
BsiSI CCGG 3 cut(s) 486, 1016, 1232
BslFI GGGAC 1 cut(s) 1041
BslI CCNNNNNNNGG 4 cut(s) 164, 492, 1147, 1262
BsmAI GTCTC 4 cut(s) 468, 722, 1182, 1277
BsmFI GGGAC 1 cut(s) 1041
BsmI GAATGC 2 cut(s) 480, 831
BsnI GGCC 7 cut(s) 28, 130, 192, 723, 1100, 1140, 1151
Bso31I GGTCTC 1 cut(s) 722
BsoBI CYCGRG 1 cut(s) 620
Bsp1286I GDGCHC 1 cut(s) 110
Bsp143I GATC 5 cut(s) 296, 349, 389, 601, 987
BspACI CCGC 2 cut(s) 374, 735
BspANI GGCC 7 cut(s) 28, 130, 192, 723, 1100, 1140, 1151
BspCNI CTCAG 4 cut(s) 453, 667, 796, 1108
BspLI GGNNCC 2 cut(s) 976, 1029
BspPI GGATC 2 cut(s) 609, 982
BspTNI GGTCTC 1 cut(s) 722
BsrDI GCAATG 1 cut(s) 586
BsrI ACTGG 3 cut(s) 52, 564, 682
BssECI CCNNGG 1 cut(s) 475
BssMI GATC 5 cut(s) 296, 349, 389, 601, 987
BssSI CACGAG 1 cut(s) 792
BssT1I CCWWGG 1 cut(s) 475
Bst2BI CACGAG 1 cut(s) 792
Bst6I CTCTTC 2 cut(s) 187, 453
BstAPI GCANNNNNTGC 1 cut(s) 1012
BstC8I GCNNGC 6 cut(s) 132, 721, 758, 789, 1084, 1126
BstDEI CTNAG 5 cut(s) 144, 440, 675, 804, 1095
BstF5I GGATG 3 cut(s) 392, 832, 1072
BstH2I RGCGCY 1 cut(s) 1125
BstHHI GCGC 3 cut(s) 579, 897, 1124
BstKTI GATC 5 cut(s) 299, 352, 392, 604, 990
BstMAI GTCTC 4 cut(s) 468, 722, 1182, 1277
BstMBI GATC 5 cut(s) 296, 349, 389, 601, 987
BstMWI GCNNNNNNNGC 4 cut(s) 486, 743, 828, 1012
BstNSI RCATGY 2 cut(s) 106, 932
BstSCI CCNGG 2 cut(s) 484, 1230
BstSFI CTRYAG 1 cut(s) 279
BstSLI GKGCMC 1 cut(s) 110
BstV1I GCAGC 3 cut(s) 757, 1018, 1024
BstX2I RGATCY 3 cut(s) 296, 601, 987
BstYI RGATCY 3 cut(s) 296, 601, 987
Bsu36I CCTNAGG 1 cut(s) 1095
BsuRI GGCC 7 cut(s) 28, 130, 192, 723, 1100, 1140, 1151
BtsCI GGATG 3 cut(s) 392, 832, 1072
BtsI GCAGTG 1 cut(s) 890
BtsIMutI CAGTG 1 cut(s) 890
Cac8I GCNNGC 6 cut(s) 132, 721, 758, 789, 1084, 1126
CfoI GCGC 3 cut(s) 579, 897, 1124
Cfr13I GGNCC 3 cut(s) 26, 679, 1028
CseI GACGC 1 cut(s) 273
Csp6I GTAC 1 cut(s) 901
CviAII CATG 8 cut(s) 103, 725, 750, 859, 929, 1153, 1262, 1277
CviQI GTAC 1 cut(s) 901
DdeI CTNAG 5 cut(s) 144, 440, 675, 804, 1095
DpnI GATC 5 cut(s) 298, 351, 391, 603, 989
DpnII GATC 5 cut(s) 296, 349, 389, 601, 987
EaeI YGGCCR 4 cut(s) 190, 721, 1138, 1149
Eam1104I CTCTTC 2 cut(s) 187, 453
EarI CTCTTC 2 cut(s) 187, 453
Eco130I CCWWGG 1 cut(s) 475
Eco147I AGGCCT 2 cut(s) 130, 1100
Eco31I GGTCTC 1 cut(s) 722
Eco47I GGWCC 2 cut(s) 679, 1028
Eco81I CCTNAGG 1 cut(s) 1095
Eco88I CYCGRG 1 cut(s) 620
EcoO109I RGGNCCY 1 cut(s) 1028
EcoRI GAATTC 2 cut(s) 230, 1071
EcoT14I CCWWGG 1 cut(s) 475
EcoT22I ATGCAT 1 cut(s) 934
ErhI CCWWGG 1 cut(s) 475
FaeI CATG 8 cut(s) 106, 728, 753, 862, 932, 1156, 1265, 1280
FalI AAGNNNNNCTT 4 cut(s) 118, 150, 459, 491
FaqI GGGAC 1 cut(s) 1041
FatI CATG 8 cut(s) 102, 724, 749, 858, 928, 1152, 1261, 1276
FbaI TGATCA 1 cut(s) 349
Fnu4HI GCNGC 3 cut(s) 771, 1007, 1038
FokI GGATG 3 cut(s) 379, 839, 1079
Fsp4HI GCNGC 3 cut(s) 771, 1007, 1038
FspBI CTAG 1 cut(s) 533
GlaI GCGC 3 cut(s) 578, 896, 1123
GluI GCNGC 3 cut(s) 771, 1007, 1038
GsuI CTGGAG 1 cut(s) 581
HaeII RGCGCY 1 cut(s) 1125
HaeIII GGCC 7 cut(s) 28, 130, 192, 723, 1100, 1140, 1151
HapII CCGG 3 cut(s) 486, 1016, 1232
HgaI GACGC 1 cut(s) 273
HhaI GCGC 3 cut(s) 579, 897, 1124
Hin1II CATG 8 cut(s) 106, 728, 753, 862, 932, 1156, 1265, 1280
Hin6I GCGC 3 cut(s) 577, 895, 1122
HinP1I GCGC 3 cut(s) 577, 895, 1122
HindIII AAGCTT 1 cut(s) 364
HinfI GANTC 7 cut(s) 275, 445, 529, 544, 880, 1059, 1171
HpaII CCGG 3 cut(s) 486, 1016, 1232
HphI GGTGA 5 cut(s) 28, 100, 889, 897, 1180
Hpy166II GTNNAC 2 cut(s) 108, 274
Hpy188I TCNGA 3 cut(s) 643, 676, 987
Hpy188III TCNNGA 7 cut(s) 227, 353, 387, 442, 533, 779, 884
Hpy8I GTNNAC 2 cut(s) 108, 274
HpyAV CCTTC 4 cut(s) 85, 120, 592, 1159
HpyCH4V TGCA 9 cut(s) 108, 569, 702, 773, 822, 932, 1006, 1086, 1217
HpyF10VI GCNNNNNNNGC 4 cut(s) 486, 743, 828, 1012
HpyF3I CTNAG 5 cut(s) 144, 440, 675, 804, 1095
Hsp92II CATG 8 cut(s) 106, 728, 753, 862, 932, 1156, 1265, 1280
HspAI GCGC 3 cut(s) 577, 895, 1122
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 5 cut(s) 296, 349, 389, 601, 987
LmnI GCTCC 1 cut(s) 626
Lsp1109I GCAGC 3 cut(s) 757, 1018, 1024
LweI GCATC 3 cut(s) 634, 755, 1024
MaeI CTAG 1 cut(s) 533
MaeIII GTNAC 3 cut(s) 70, 201, 285
MalI GATC 5 cut(s) 298, 351, 391, 603, 989
MboI GATC 5 cut(s) 296, 349, 389, 601, 987
MboII GAAGA 6 cut(s) 25, 174, 440, 596, 599, 950
MfeI CAATTG 1 cut(s) 1010
MflI RGATCY 3 cut(s) 296, 601, 987
MhlI GDGCHC 1 cut(s) 110
MlsI TGGCCA 4 cut(s) 192, 723, 1140, 1151
MluNI TGGCCA 4 cut(s) 192, 723, 1140, 1151
MlyI GAGTC 3 cut(s) 269, 439, 538
Mox20I TGGCCA 4 cut(s) 192, 723, 1140, 1151
Mph1103I ATGCAT 1 cut(s) 934
MscI TGGCCA 4 cut(s) 192, 723, 1140, 1151
MseI TTAA 6 cut(s) 65, 249, 266, 293, 945, 1047
MslI CAYNNNNRTG 1 cut(s) 751
Msp20I TGGCCA 4 cut(s) 192, 723, 1140, 1151
MspA1I CMGCKG 1 cut(s) 374
MspI CCGG 3 cut(s) 486, 1016, 1232
MspR9I CCNGG 2 cut(s) 486, 1232
MunI CAATTG 1 cut(s) 1010
Mva1269I GAATGC 2 cut(s) 480, 831
MwoI GCNNNNNNNGC 4 cut(s) 486, 743, 828, 1012
NciI CCSGG 2 cut(s) 486, 1232
NdeII GATC 5 cut(s) 296, 349, 389, 601, 987
NlaIII CATG 8 cut(s) 106, 728, 753, 862, 932, 1156, 1265, 1280
NlaIV GGNNCC 2 cut(s) 976, 1029
NmuCI GTSAC 2 cut(s) 201, 285
NsiI ATGCAT 1 cut(s) 934
NspI RCATGY 2 cut(s) 106, 932
PaeR7I CTCGAG 1 cut(s) 620
PceI AGGCCT 2 cut(s) 130, 1100
PctI GAATGC 2 cut(s) 480, 831
PfeI GAWTC 4 cut(s) 544, 880, 1059, 1171
PflMI CCANNNNNTGG 1 cut(s) 1147
PkrI GCNGC 3 cut(s) 772, 1008, 1039
PleI GAGTC 3 cut(s) 269, 439, 537
PpsI GAGTC 3 cut(s) 269, 439, 537
PpuMI RGGWCCY 1 cut(s) 1028
Psp5II RGGWCCY 1 cut(s) 1028
PspN4I GGNNCC 2 cut(s) 976, 1029
PspPI GGNCC 3 cut(s) 26, 679, 1028
PspPPI RGGWCCY 1 cut(s) 1028
PspXI VCTCGAGB 1 cut(s) 620
PsuI RGATCY 3 cut(s) 296, 601, 987
RsaI GTAC 1 cut(s) 902
RsaNI GTAC 1 cut(s) 901
RseI CAYNNNNRTG 1 cut(s) 751
SaqAI TTAA 6 cut(s) 65, 249, 266, 293, 945, 1047
SatI GCNGC 3 cut(s) 771, 1007, 1038
Sau3AI GATC 5 cut(s) 296, 349, 389, 601, 987
Sau96I GGNCC 3 cut(s) 26, 679, 1028
SchI GAGTC 3 cut(s) 269, 439, 538
ScrFI CCNGG 2 cut(s) 486, 1232
SduI GDGCHC 1 cut(s) 110
SfaNI GCATC 3 cut(s) 634, 755, 1024
SfcI CTRYAG 1 cut(s) 279
Sfr274I CTCGAG 1 cut(s) 620
SinI GGWCC 2 cut(s) 679, 1028
SlaI CTCGAG 1 cut(s) 620
SmiMI CAYNNNNRTG 1 cut(s) 751
SmlI CTYRAG 2 cut(s) 465, 620
SmoI CTYRAG 2 cut(s) 465, 620
SseBI AGGCCT 2 cut(s) 130, 1100
SsiI CCGC 2 cut(s) 374, 735
SspMI CTAG 1 cut(s) 533
StuI AGGCCT 2 cut(s) 130, 1100
StyD4I CCNGG 2 cut(s) 484, 1230
StyI CCWWGG 1 cut(s) 475
TaqI TCGA 5 cut(s) 261, 621, 853, 1062, 1209
TatI WGTACW 1 cut(s) 900
TfiI GAWTC 4 cut(s) 544, 880, 1059, 1171
Tru1I TTAA 6 cut(s) 65, 249, 266, 293, 945, 1047
Tru9I TTAA 6 cut(s) 65, 249, 266, 293, 945, 1047
TscAI CASTG 1 cut(s) 897
TseFI GTSAC 2 cut(s) 201, 285
TseI GCWGC 3 cut(s) 770, 1006, 1037
Tsp45I GTSAC 2 cut(s) 201, 285
TspDTI ATGAA 4 cut(s) 651, 676, 875, 907
TspRI CASTG 1 cut(s) 897
Van91I CCANNNNNTGG 1 cut(s) 1147
VneI GTGCAC 1 cut(s) 106
VpaK11BI GGWCC 2 cut(s) 679, 1028
XapI RAATTY 3 cut(s) 230, 816, 1071
XbaI TCTAGA 1 cut(s) 532
XceI RCATGY 2 cut(s) 106, 932
XhoI CTCGAG 1 cut(s) 620
XspI CTAG 1 cut(s) 533
Zsp2I ATGCAT 1 cut(s) 934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.