Rorug01G0175400

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0005196
Physical Location & Seq
Forward (+)
23848844 .. 23856309
7466 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0175400.1

Sequence Viewer

Length: 624 bp
ATGGTGAACCGAAATTCCTCATGTTGTTCTTCTCCACTCAGAATCCAAATCCTCTTAGTATTGTCGTTGCTATTCTCTCCCGCAGTTCTCGCCAAGTCCCGTCGCCCAATCAGTGACATTGAGACAAGGCAGAAGAAGACCCAATGCTATGCTGATATTGACAGTGGCTTGTGGGGATGGCAATGCAAGTCAACTATGATAGCAAAGGAGAATTGTGTTTTGAAATGCCTCTCTCCAACTTGTTATGAGCTCGTCTATGAGAGCGATCCGCTTGAAGAAGGAGAGAAAGATCTTCTTAGAGGACAAGAGTTCAAGTACTGTATGCATAAAAGGCCTCCTTATGGAGGAAATAAATTTGGTGCTGGTGTGGAGTACTGCGCTTGGAGTTTTGTTCCCAGAGATTGCAACAAAGTAGCGCATGAAGCTGCAAAGCATGCCTTAGCAATTAGTTTTGCAGTTGATAGGAGGCGCAACTCCTTGGCCAATTGTTCCAATCCATCAAGACAGTTTGTCAAGATCGATAGTCAAATTCTTGTTATAATAGTTACGATAGAGTTCACGAACATGCTGAGAAGTTTACCAAATTTTAACAGCAGAGAGGGAAATATCAAGGATCTTAGCTAG

Protein Analysis

207

Amino Acids

23.4

Weight (kDa)

8.86

Isoelectric Point (pI)

60.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4787 PF16029 39 - 107 3.3e-24 Domain of unknown function (DUF4787)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 539
AciI CCGC 2 cut(s) 81, 269
AclWI GGATC 2 cut(s) 260, 621
AcoI YGGCCR 1 cut(s) 480
AcsI RAATTY 4 cut(s) 13, 353, 528, 583
AfaI GTAC 2 cut(s) 317, 374
AfiI CCNNNNNNNGG 2 cut(s) 341, 344
AgsI TTSAA 3 cut(s) 223, 275, 313
AhdI GACNNNNNGTC 1 cut(s) 509
AloI GAACNNNNNNTCC 1 cut(s) 31
AluBI AGCT 3 cut(s) 250, 425, 621
AluI AGCT 3 cut(s) 250, 425, 621
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 1 cut(s) 116
AlwI GGATC 2 cut(s) 260, 621
AoxI GGCC 2 cut(s) 332, 480
ApeKI GCWGC 1 cut(s) 425
ApoI RAATTY 4 cut(s) 13, 353, 528, 583
AspLEI GCGC 3 cut(s) 380, 418, 471
AsuHPI GGTGA 1 cut(s) 16
BalI TGGCCA 1 cut(s) 482
BanII GRGCYC 1 cut(s) 252
BbsI GAAGAC 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 252
BbvI GCAGC 1 cut(s) 412
BccI CCATC 2 cut(s) 171, 505
BcoDI GTCTC 1 cut(s) 116
BfaI CTAG 1 cut(s) 622
BglII AGATCT 1 cut(s) 289
BisI GCNGC 1 cut(s) 426
BlsI GCNGC 1 cut(s) 427
BmcAI AGTACT 2 cut(s) 317, 374
BmeRI GACNNNNNGTC 1 cut(s) 509
BpiI GAAGAC 1 cut(s) 143
Bpu10I CCTNAGC 1 cut(s) 439
Bsa29I ATCGAT 1 cut(s) 519
BsaJI CCNNGG 1 cut(s) 477
Bsc4I CCNNNNNNNGG 2 cut(s) 341, 344
Bse3DI GCAATG 1 cut(s) 188
BseCI ATCGAT 1 cut(s) 519
BseDI CCNNGG 1 cut(s) 477
BseGI GGATG 1 cut(s) 182
BseLI CCNNNNNNNGG 2 cut(s) 341, 344
BseMI GCAATG 1 cut(s) 188
BseMII CTCAG 2 cut(s) 52, 560
BseXI GCAGC 1 cut(s) 412
BshFI GGCC 2 cut(s) 334, 482
BshVI ATCGAT 1 cut(s) 519
BsiHKAI GWGCWC 1 cut(s) 252
BslFI GGGAC 1 cut(s) 82
BslI CCNNNNNNNGG 2 cut(s) 341, 344
BsmAI GTCTC 1 cut(s) 116
BsmFI GGGAC 1 cut(s) 82
BsnI GGCC 2 cut(s) 334, 482
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 4 cut(s) 265, 289, 516, 613
BspACI CCGC 2 cut(s) 81, 269
BspANI GGCC 2 cut(s) 334, 482
BspCNI CTCAG 2 cut(s) 51, 561
BspDI ATCGAT 1 cut(s) 519
BspPI GGATC 2 cut(s) 260, 621
BsrDI GCAATG 1 cut(s) 188
BssECI CCNNGG 1 cut(s) 477
BssMI GATC 4 cut(s) 265, 289, 516, 613
BssT1I CCWWGG 1 cut(s) 477
Bst4CI ACNGT 3 cut(s) 164, 320, 507
BstAPI GCANNNNNTGC 1 cut(s) 434
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 6 cut(s) 38, 55, 296, 439, 569, 617
BstENI CCTNNNNNAGG 1 cut(s) 342
BstF5I GGATG 1 cut(s) 182
BstHHI GCGC 3 cut(s) 380, 418, 471
BstKTI GATC 4 cut(s) 268, 292, 519, 616
BstMAI GTCTC 1 cut(s) 116
BstMBI GATC 4 cut(s) 265, 289, 516, 613
BstMWI GCNNNNNNNGC 4 cut(s) 89, 331, 422, 434
BstNSI RCATGY 2 cut(s) 437, 568
BstV1I GCAGC 1 cut(s) 412
BstV2I GAAGAC 1 cut(s) 143
BstX2I RGATCY 2 cut(s) 289, 613
BstYI RGATCY 2 cut(s) 289, 613
Bsu15I ATCGAT 1 cut(s) 519
BsuRI GGCC 2 cut(s) 334, 482
BsuTUI ATCGAT 1 cut(s) 519
BtsCI GGATG 1 cut(s) 182
BtsIMutI CAGTG 2 cut(s) 118, 169
Cac8I GCNNGC 1 cut(s) 435
CfoI GCGC 3 cut(s) 380, 418, 471
ClaI ATCGAT 1 cut(s) 519
Csp6I GTAC 2 cut(s) 316, 373
CviAII CATG 4 cut(s) 21, 419, 434, 565
CviJI RGCY 6 cut(s) 168, 250, 334, 425, 482, 621
CviKI_1 RGCY 6 cut(s) 168, 250, 334, 425, 482, 621
CviQI GTAC 2 cut(s) 316, 373
DdeI CTNAG 6 cut(s) 38, 55, 296, 439, 569, 617
DpnI GATC 4 cut(s) 267, 291, 518, 615
DpnII GATC 4 cut(s) 265, 289, 516, 613
DriI GACNNNNNGTC 1 cut(s) 509
EaeI YGGCCR 1 cut(s) 480
Eam1105I GACNNNNNGTC 1 cut(s) 509
Ecl136II GAGCTC 1 cut(s) 250
Eco130I CCWWGG 1 cut(s) 477
Eco147I AGGCCT 1 cut(s) 334
Eco24I GRGCYC 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 250
EcoICRI GAGCTC 1 cut(s) 250
EcoNI CCTNNNNNAGG 1 cut(s) 342
EcoT14I CCWWGG 1 cut(s) 477
EcoT22I ATGCAT 1 cut(s) 327
EcoT38I GRGCYC 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 477
FaeI CATG 4 cut(s) 24, 422, 437, 568
FalI AAGNNNNNCTT 6 cut(s) 279, 311, 322, 354, 422, 454
FaqI GGGAC 1 cut(s) 82
FatI CATG 4 cut(s) 20, 418, 433, 564
FauI CCCGC 1 cut(s) 88
Fnu4HI GCNGC 1 cut(s) 426
FokI GGATG 1 cut(s) 189
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 1 cut(s) 426
FspBI CTAG 1 cut(s) 622
GlaI GCGC 3 cut(s) 379, 417, 470
GluI GCNGC 1 cut(s) 426
HaeIII GGCC 2 cut(s) 334, 482
HhaI GCGC 3 cut(s) 380, 418, 471
Hin1II CATG 4 cut(s) 24, 422, 437, 568
Hin6I GCGC 3 cut(s) 378, 416, 469
HinP1I GCGC 3 cut(s) 378, 416, 469
HincII GTYRAC 1 cut(s) 192
HindII GTYRAC 1 cut(s) 192
HinfI GANTC 1 cut(s) 42
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 4 cut(s) 7, 192, 558, 578
Hpy188I TCNGA 1 cut(s) 41
Hpy188III TCNNGA 3 cut(s) 501, 514, 559
Hpy8I GTNNAC 4 cut(s) 7, 192, 558, 578
Hpy99I CGWCG 1 cut(s) 105
HpyAV CCTTC 1 cut(s) 272
HpyCH4III ACNGT 3 cut(s) 164, 320, 507
HpyCH4V TGCA 5 cut(s) 186, 325, 405, 428, 455
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 331, 422, 434
HpyF3I CTNAG 6 cut(s) 38, 55, 296, 439, 569, 617
Hsp92II CATG 4 cut(s) 24, 422, 437, 568
HspAI GCGC 3 cut(s) 378, 416, 469
Kzo9I GATC 4 cut(s) 265, 289, 516, 613
LpnPI CCDG 2 cut(s) 348, 409
Lsp1109I GCAGC 1 cut(s) 412
MaeI CTAG 1 cut(s) 622
MaeIII GTNAC 2 cut(s) 113, 544
MalI GATC 4 cut(s) 267, 291, 518, 615
MboI GATC 4 cut(s) 265, 289, 516, 613
MboII GAAGA 5 cut(s) 21, 145, 148, 284, 287
MfeI CAATTG 1 cut(s) 484
MflI RGATCY 2 cut(s) 289, 613
MhlI GDGCHC 1 cut(s) 252
MlsI TGGCCA 1 cut(s) 482
MluCI AATT 7 cut(s) 13, 211, 353, 444, 484, 528, 583
MluNI TGGCCA 1 cut(s) 482
MmeI TCCRAC 1 cut(s) 260
MnlI CCTC 8 cut(s) 28, 62, 239, 293, 338, 345, 459, 592
Mox20I TGGCCA 1 cut(s) 482
Mph1103I ATGCAT 1 cut(s) 327
MscI TGGCCA 1 cut(s) 482
MseI TTAA 1 cut(s) 588
MslI CAYNNNNRTG 1 cut(s) 563
Msp20I TGGCCA 1 cut(s) 482
MunI CAATTG 1 cut(s) 484
MwoI GCNNNNNNNGC 4 cut(s) 89, 331, 422, 434
NdeII GATC 4 cut(s) 265, 289, 516, 613
NlaIII CATG 4 cut(s) 24, 422, 437, 568
NmuCI GTSAC 1 cut(s) 113
NsiI ATGCAT 1 cut(s) 327
NspI RCATGY 2 cut(s) 437, 568
PaeI GCATGC 1 cut(s) 437
PceI AGGCCT 1 cut(s) 334
PfeI GAWTC 1 cut(s) 42
PkrI GCNGC 1 cut(s) 427
PsiI TTATAA 1 cut(s) 539
Psp124BI GAGCTC 1 cut(s) 252
PsuI RGATCY 2 cut(s) 289, 613
RsaI GTAC 2 cut(s) 317, 374
RsaNI GTAC 2 cut(s) 316, 373
RseI CAYNNNNRTG 1 cut(s) 563
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 1 cut(s) 588
SatI GCNGC 1 cut(s) 426
Sau3AI GATC 4 cut(s) 265, 289, 516, 613
ScaI AGTACT 2 cut(s) 317, 374
SduI GDGCHC 1 cut(s) 252
SetI ASST 3 cut(s) 252, 427, 623
SmiMI CAYNNNNRTG 1 cut(s) 563
SphI GCATGC 1 cut(s) 437
Sse9I AATT 7 cut(s) 13, 211, 353, 444, 484, 528, 583
SseBI AGGCCT 1 cut(s) 334
SsiI CCGC 2 cut(s) 81, 269
SspMI CTAG 1 cut(s) 622
SstI GAGCTC 1 cut(s) 252
StuI AGGCCT 1 cut(s) 334
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 3 cut(s) 164, 320, 507
TaqI TCGA 1 cut(s) 519
TasI AATT 7 cut(s) 13, 211, 353, 444, 484, 528, 583
TatI WGTACW 2 cut(s) 315, 372
TfiI GAWTC 1 cut(s) 42
Tru1I TTAA 1 cut(s) 588
Tru9I TTAA 1 cut(s) 588
TscAI CASTG 2 cut(s) 118, 169
TseFI GTSAC 1 cut(s) 113
TseI GCWGC 1 cut(s) 425
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 1 cut(s) 435
TspRI CASTG 2 cut(s) 118, 169
XagI CCTNNNNNAGG 1 cut(s) 342
XapI RAATTY 4 cut(s) 13, 353, 528, 583
XceI RCATGY 2 cut(s) 437, 568
XspI CTAG 1 cut(s) 622
ZrmI AGTACT 2 cut(s) 317, 374
Zsp2I ATGCAT 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.