MD02G1221300.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005196
Physical Location & Seq
Reverse (-)
25713040 .. 25715506
2467 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1221300.v1.1.491

Sequence Viewer

Length: 1593 bp
ATGGCAGCAGCTGAGATGACAGTATCAGCACTGCTGGGAGGCTTTGTGTTATTCGTATTATATGTCTATGAATCTTTGGTCTTAAAGCCAAAGAGGCTAAGATCAAAGCTTGAAAAGCAAGGGATTAGAGGGCCTCCCCCTTCATCAATTCTCTTCGGCAATATCCCGGACATGAACAGCATCAAACTCAAGGTGATGAGGGAGAAGTCAACTGCTACGGAAACAAGTAGTACCAGTGTCTCAAAAGACCGGCATGTTTTGATTGATCATGATTGGCCTTCAACCCTTTTCCCTCATCTCGTGCAATGGCGAAACCACTTTGGGCCAAACTTCACATATTTAACTGGAAGCATACAGCAATTAAGCATAACTGATTTAGAAATGGTGAAGGAAGTTTGTTTGTGTAGATCTTTGAACTTAGGGAGGCCTACTTTTGTATCTAAGGATCGTAAGCCACTGTTCGGCCAAGGAATTATAGCGTCAAATGGTCCAATTTGGTTGCACCAAAAAAAAATTATTGGTCCAGAGTTCTACTTTGAAAAGGTTAAGGGTATGGTGGATTTGATGGTGGACTCCACAACCACCATGTTGAGATCTTGGGAGAGTAAAATCGAAAACGAAGGAGGAAGTGCAGTCTTTGAAGTTGATAAGGATTTGAGAAGCTTGTCGGCCGATATTATATCAAGAACGTCCTTTGGAAGTAATTATTCACAAGGGAAAGAAATATTCTTAAAGCTCAGAACTCTTCAGAAGATCATGTCCCAGGGAAATACTGGTGTACCGGGTGCGAGACACTTACCAACGAAAAACAACAGACTGATATGGAGACTAGAGAAAGAGATCAGTGCTATGATTTTAAGGGTTGCGAAGGAACGCAGTACTGAGGCTACTTGTGACAAGGATCTTCTGCAAATAATACTCGAGGGAGCCAAAAATTATGAAGACGCTAATAGCCTATTTTCAGGTATATCCCAGGATAGTTTTATAGTGGATAATTGCAAGAGTATATACTTTGCTGGCCACGAAACCACTGCCACAGCAGCGTCTTGGAGCTTGATGTTATTAGCTGCACATCAAGACTGGCAAACTTGTGTCCGTGCTGAGGTGCTTGAATTTTGTAGGGATGGCATTCCAGATGCTGATTTGCTTCGAAACATGAAAATATTGAATATGGTGATTCAAGAGACACTGCGCCTGTACTCACCATCAACGGTTGTTGCAAGAGAAGCCTTGGAAGATATTGAGCTGAAAGGCATTCTAATTCCCAAAGGAACGAACATTCAGATTCCAATTCCTATAGTGCATCGACTTCCTGATATCTGGGGTCCCGATGGCCATGAATTCAATCCAAAAAGATTCGAGCATGGAATTGCTGGGGCTTGCAAGTTTCCTCAGGCTTATATGCCATTTGGAGTTGGTGCCCGTATCTGTACTGGCCAACACTTAGCCATGACAGAGCTGAAGGTGATTCTGTCACTTATTCTGTCCAAGTTTTCCTTCTCACTCTCACCAGAATACCAACACTCCCCGGCTTATACGTTGGTTATTATGGAACCCGAACATGGAGTTAGTCTCCATATGAGGAGAGTGTGA

Protein Analysis

531

Amino Acids

59.47

Weight (kDa)

8.61

Isoelectric Point (pI)

37.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 99 - 517 1.2e-74 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1418
AclWI GGATC 2 cut(s) 453, 909
AcoI YGGCCR 5 cut(s) 463, 669, 1018, 1333, 1435
AcsI RAATTY 2 cut(s) 1112, 1340
AcuI CTGAAG 2 cut(s) 731, 1481
AfaI GTAC 5 cut(s) 232, 780, 880, 1199, 1432
AfiI CCNNNNNNNGG 3 cut(s) 461, 1102, 1562
AgsI TTSAA 9 cut(s) 113, 282, 415, 539, 641, 1112, 1168, 1181, 1345
AjnI CCWGG 2 cut(s) 762, 972
AjuI GAANNNNNNNTTGG 2 cut(s) 1481, 1513
AluBI AGCT 8 cut(s) 11, 109, 663, 736, 1053, 1067, 1246, 1459
AluI AGCT 8 cut(s) 11, 109, 663, 736, 1053, 1067, 1246, 1459
Alw26I GTCTC 5 cut(s) 244, 784, 820, 1178, 1577
AlwI GGATC 2 cut(s) 453, 909
AlwNI CAGNNNCTG 2 cut(s) 11, 1139
Ama87I CYCGRG 1 cut(s) 920
AoxI GGCC 9 cut(s) 131, 275, 323, 425, 463, 669, 1018, 1333, 1435
ApeKI GCWGC 4 cut(s) 5, 8, 1040, 1067
ApoI RAATTY 2 cut(s) 1112, 1340
AspLEI GCGC 1 cut(s) 1194
AspS9I GGNCC 5 cut(s) 131, 323, 488, 521, 1325
AsuC2I CCSGG 3 cut(s) 167, 783, 1529
AsuHPI GGTGA 6 cut(s) 205, 397, 1186, 1194, 1477, 1500
AsuII TTCGAA 1 cut(s) 1150
AvaI CYCGRG 1 cut(s) 920
AvaII GGWCC 3 cut(s) 488, 521, 1325
AxyI CCTNAGG 1 cut(s) 1392
BaeGI GKGCMC 1 cut(s) 1423
BaeI ACNNNNGTAYC 4 cut(s) 420, 453, 762, 795
BalI TGGCCA 3 cut(s) 1020, 1335, 1437
BanI GGYRCC 1 cut(s) 1418
BauI CACGAG 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 948
BbvCI CCTCAGC 1 cut(s) 1101
BbvI GCAGC 4 cut(s) 17, 20, 1052, 1054
BccI CCATC 4 cut(s) 559, 1118, 1213, 1325
BcgI CGANNNNNNTGC 2 cut(s) 1013, 1047
BciT130I CCWGG 2 cut(s) 764, 974
BclI TGATCA 1 cut(s) 265
BcnI CCSGG 3 cut(s) 167, 783, 1529
BcoDI GTCTC 5 cut(s) 244, 784, 820, 1178, 1577
BfaI CTAG 1 cut(s) 830
BfmI CTRYAG 1 cut(s) 1296
BglI GCCNNNNNGGC 1 cut(s) 94
BglII AGATCT 2 cut(s) 407, 593
BisI GCNGC 4 cut(s) 6, 9, 1041, 1068
BlsI GCNGC 4 cut(s) 7, 10, 1042, 1069
BmcAI AGTACT 1 cut(s) 880
Bme1390I CCNGG 5 cut(s) 167, 764, 783, 974, 1529
Bme18I GGWCC 3 cut(s) 488, 521, 1325
BmeT110I CYCGRG 1 cut(s) 920
BmgT120I GGNCC 5 cut(s) 131, 323, 488, 521, 1325
BmiI GGNNCC 5 cut(s) 928, 1326, 1327, 1420, 1554
BmrFI CCNGG 5 cut(s) 167, 764, 783, 974, 1529
BmsI GCATC 3 cut(s) 189, 1126, 1312
BpiI GAAGAC 1 cut(s) 948
BplI GAGNNNNNCTC 2 cut(s) 1557, 1589
Bpu10I CCTNAGC 1 cut(s) 1101
Bpu14I TTCGAA 1 cut(s) 1150
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 3 cut(s) 167, 783, 1529
BsaJI CCNNGG 6 cut(s) 466, 762, 763, 972, 1230, 1527
BsaXI ACNNNNNCTCC 4 cut(s) 30, 60, 1508, 1538
Bsc4I CCNNNNNNNGG 3 cut(s) 461, 1102, 1562
Bse118I RCCGGY 1 cut(s) 249
Bse1I ACTGG 5 cut(s) 234, 349, 778, 1085, 1438
Bse21I CCTNAGG 1 cut(s) 1392
Bse3DI GCAATG 1 cut(s) 311
BseBI CCWGG 2 cut(s) 764, 974
BseDI CCNNGG 6 cut(s) 466, 762, 763, 972, 1230, 1527
BseGI GGATG 1 cut(s) 1129
BseLI CCNNNNNNNGG 3 cut(s) 461, 1102, 1562
BseMI GCAATG 1 cut(s) 311
BseMII CTCAG 4 cut(s) 751, 873, 1092, 1406
BseNI ACTGG 5 cut(s) 234, 349, 778, 1085, 1438
BseSI GKGCMC 1 cut(s) 1423
BseX3I CGGCCG 1 cut(s) 669
BseXI GCAGC 4 cut(s) 17, 20, 1052, 1054
BseYI CCCAGC 2 cut(s) 34, 1373
BsgI GTGCAG 2 cut(s) 651, 1053
Bsh1285I CGRYCG 1 cut(s) 672
BshFI GGCC 9 cut(s) 133, 277, 325, 427, 465, 671, 1020, 1335, 1437
BshNI GGYRCC 1 cut(s) 1418
BsiEI CGRYCG 1 cut(s) 672
BsiHKCI CYCGRG 1 cut(s) 920
BsiSI CCGG 4 cut(s) 167, 250, 782, 1529
BslFI GGGAC 2 cut(s) 745, 1311
BslI CCNNNNNNNGG 3 cut(s) 461, 1102, 1562
BsmAI GTCTC 5 cut(s) 244, 784, 820, 1178, 1577
BsmFI GGGAC 2 cut(s) 745, 1311
BsmI GAATGC 2 cut(s) 1128, 1254
BsnI GGCC 9 cut(s) 133, 277, 325, 427, 465, 671, 1020, 1335, 1437
BsoBI CYCGRG 1 cut(s) 920
Bsp119I TTCGAA 1 cut(s) 1150
Bsp1286I GDGCHC 1 cut(s) 1423
Bsp143I GATC 8 cut(s) 101, 265, 407, 445, 593, 753, 840, 901
BspANI GGCC 9 cut(s) 133, 277, 325, 427, 465, 671, 1020, 1335, 1437
BspCNI CTCAG 5 cut(s) 4, 750, 874, 1093, 1405
BspHI TCATGA 1 cut(s) 268
BspLI GGNNCC 5 cut(s) 928, 1326, 1327, 1420, 1554
BspPI GGATC 2 cut(s) 453, 909
BspT104I TTCGAA 1 cut(s) 1150
BspT107I GGYRCC 1 cut(s) 1418
BsrDI GCAATG 1 cut(s) 311
BsrFI RCCGGY 1 cut(s) 249
BsrI ACTGG 5 cut(s) 234, 349, 778, 1085, 1438
BssAI RCCGGY 1 cut(s) 249
BssECI CCNNGG 6 cut(s) 466, 762, 763, 972, 1230, 1527
BssMI GATC 8 cut(s) 101, 265, 407, 445, 593, 753, 840, 901
BssSI CACGAG 1 cut(s) 299
BssT1I CCWWGG 2 cut(s) 466, 1230
Bst2BI CACGAG 1 cut(s) 299
Bst2UI CCWGG 2 cut(s) 764, 974
Bst4CI ACNGT 3 cut(s) 22, 459, 1213
Bst6I CTCTTC 2 cut(s) 158, 750
BstBI TTCGAA 1 cut(s) 1150
BstC8I GCNNGC 2 cut(s) 1018, 1381
BstDEI CTNAG 9 cut(s) 12, 98, 418, 441, 737, 882, 1101, 1392, 1444
BstF5I GGATG 1 cut(s) 1129
BstHHI GCGC 1 cut(s) 1194
BstKTI GATC 8 cut(s) 104, 268, 410, 448, 596, 756, 843, 904
BstMAI GTCTC 5 cut(s) 244, 784, 820, 1178, 1577
BstMBI GATC 8 cut(s) 101, 265, 407, 445, 593, 753, 840, 901
BstMCI CGRYCG 1 cut(s) 672
BstMWI GCNNNNNNNGC 4 cut(s) 94, 115, 1040, 1226
BstNI CCWGG 2 cut(s) 764, 974
BstNSI RCATGY 1 cut(s) 257
BstSCI CCNGG 5 cut(s) 165, 762, 781, 972, 1527
BstSFI CTRYAG 1 cut(s) 1296
BstSLI GKGCMC 1 cut(s) 1423
BstV1I GCAGC 4 cut(s) 17, 20, 1052, 1054
BstV2I GAAGAC 1 cut(s) 948
BstX2I RGATCY 3 cut(s) 407, 593, 901
BstYI RGATCY 3 cut(s) 407, 593, 901
BstZI CGGCCG 1 cut(s) 669
Bsu36I CCTNAGG 1 cut(s) 1392
BsuRI GGCC 9 cut(s) 133, 277, 325, 427, 465, 671, 1020, 1335, 1437
BtsCI GGATG 1 cut(s) 1129
BtsI GCAGTG 3 cut(s) 29, 1029, 1187
BtsIMutI CAGTG 6 cut(s) 29, 241, 455, 850, 1029, 1187
Cac8I GCNNGC 2 cut(s) 1018, 1381
CaiI CAGNNNCTG 2 cut(s) 11, 1139
CciI TCATGA 1 cut(s) 268
CfoI GCGC 1 cut(s) 1194
Cfr10I RCCGGY 1 cut(s) 249
Cfr13I GGNCC 5 cut(s) 131, 323, 488, 521, 1325
CseI GACGC 3 cut(s) 468, 953, 1032
Csp6I GTAC 5 cut(s) 231, 779, 879, 1198, 1431
CviQI GTAC 5 cut(s) 231, 779, 879, 1198, 1431
DdeI CTNAG 9 cut(s) 12, 98, 418, 441, 737, 882, 1101, 1392, 1444
DpnI GATC 8 cut(s) 103, 267, 409, 447, 595, 755, 842, 903
DpnII GATC 8 cut(s) 101, 265, 407, 445, 593, 753, 840, 901
EaeI YGGCCR 5 cut(s) 463, 669, 1018, 1333, 1435
EagI CGGCCG 1 cut(s) 669
Eam1104I CTCTTC 2 cut(s) 158, 750
EarI CTCTTC 2 cut(s) 158, 750
EclXI CGGCCG 1 cut(s) 669
Eco130I CCWWGG 2 cut(s) 466, 1230
Eco147I AGGCCT 1 cut(s) 427
Eco32I GATATC 1 cut(s) 1318
Eco47I GGWCC 3 cut(s) 488, 521, 1325
Eco52I CGGCCG 1 cut(s) 669
Eco57I CTGAAG 2 cut(s) 731, 1481
Eco81I CCTNAGG 1 cut(s) 1392
Eco88I CYCGRG 1 cut(s) 920
EcoO109I RGGNCCY 2 cut(s) 131, 1325
EcoRI GAATTC 1 cut(s) 1340
EcoRII CCWGG 2 cut(s) 762, 972
EcoRV GATATC 1 cut(s) 1318
EcoT14I CCWWGG 2 cut(s) 466, 1230
ErhI CCWWGG 2 cut(s) 466, 1230
FalI AAGNNNNNCTT 2 cut(s) 1481, 1513
FaqI GGGAC 2 cut(s) 745, 1311
FauNDI CATATG 1 cut(s) 1578
FbaI TGATCA 1 cut(s) 265
Fnu4HI GCNGC 4 cut(s) 6, 9, 1041, 1068
FokI GGATG 1 cut(s) 1136
Fsp4HI GCNGC 4 cut(s) 6, 9, 1041, 1068
FspBI CTAG 1 cut(s) 830
GlaI GCGC 1 cut(s) 1193
GluI GCNGC 4 cut(s) 6, 9, 1041, 1068
GsaI CCCAGC 2 cut(s) 38, 1377
HaeIII GGCC 9 cut(s) 133, 277, 325, 427, 465, 671, 1020, 1335, 1437
HapII CCGG 4 cut(s) 167, 250, 782, 1529
HgaI GACGC 3 cut(s) 468, 953, 1032
HhaI GCGC 1 cut(s) 1194
Hin6I GCGC 1 cut(s) 1192
HinP1I GCGC 1 cut(s) 1192
HincII GTYRAC 1 cut(s) 210
HindII GTYRAC 1 cut(s) 210
HindIII AAGCTT 2 cut(s) 107, 661
HinfI GANTC 6 cut(s) 71, 572, 1177, 1285, 1356, 1468
HpaII CCGG 4 cut(s) 167, 250, 782, 1529
HphI GGTGA 6 cut(s) 205, 397, 1186, 1194, 1477, 1500
Hpy166II GTNNAC 3 cut(s) 210, 571, 779
Hpy188I TCNGA 3 cut(s) 740, 750, 1284
Hpy188III TCNNGA 8 cut(s) 269, 524, 684, 1076, 1133, 1181, 1313, 1328
Hpy8I GTNNAC 3 cut(s) 210, 571, 779
HpyAV CCTTC 7 cut(s) 150, 288, 382, 614, 862, 1456, 1507
HpyCH4III ACNGT 3 cut(s) 22, 459, 1213
HpyCH4IV ACGT 2 cut(s) 689, 1538
HpyCH4V TGCA 9 cut(s) 304, 502, 632, 910, 999, 1070, 1220, 1303, 1383
HpyF10VI GCNNNNNNNGC 4 cut(s) 94, 115, 1040, 1226
HpyF3I CTNAG 9 cut(s) 12, 98, 418, 441, 737, 882, 1101, 1392, 1444
HpySE526I ACGT 2 cut(s) 689, 1538
HspAI GCGC 1 cut(s) 1192
KflI GGGWCCC 1 cut(s) 1325
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 8 cut(s) 101, 265, 407, 445, 593, 753, 840, 901
LmnI GCTCC 2 cut(s) 926, 1050
Lsp1109I GCAGC 4 cut(s) 17, 20, 1052, 1054
LweI GCATC 3 cut(s) 189, 1126, 1312
MaeI CTAG 1 cut(s) 830
MaeII ACGT 2 cut(s) 689, 1538
MaeIII GTNAC 2 cut(s) 893, 1473
MalI GATC 8 cut(s) 103, 267, 409, 447, 595, 755, 842, 903
MboI GATC 8 cut(s) 101, 265, 407, 445, 593, 753, 840, 901
MboII GAAGA 6 cut(s) 145, 737, 763, 896, 953, 1247
MflI RGATCY 3 cut(s) 407, 593, 901
MhlI GDGCHC 1 cut(s) 1423
MlsI TGGCCA 3 cut(s) 1020, 1335, 1437
MluNI TGGCCA 3 cut(s) 1020, 1335, 1437
MlyI GAGTC 1 cut(s) 566
Mox20I TGGCCA 3 cut(s) 1020, 1335, 1437
MscI TGGCCA 3 cut(s) 1020, 1335, 1437
MseI TTAA 6 cut(s) 83, 341, 362, 546, 731, 857
Msp20I TGGCCA 3 cut(s) 1020, 1335, 1437
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 4 cut(s) 167, 250, 782, 1529
MspR9I CCNGG 5 cut(s) 167, 764, 783, 974, 1529
Mva1269I GAATGC 2 cut(s) 1128, 1254
MvaI CCWGG 2 cut(s) 764, 974
MwoI GCNNNNNNNGC 4 cut(s) 94, 115, 1040, 1226
NciI CCSGG 3 cut(s) 167, 783, 1529
NdeI CATATG 1 cut(s) 1578
NdeII GATC 8 cut(s) 101, 265, 407, 445, 593, 753, 840, 901
NlaIV GGNNCC 5 cut(s) 928, 1326, 1327, 1420, 1554
NmuCI GTSAC 2 cut(s) 893, 1473
NspI RCATGY 1 cut(s) 257
NspV TTCGAA 1 cut(s) 1150
PaeR7I CTCGAG 1 cut(s) 920
PagI TCATGA 1 cut(s) 268
PasI CCCWGGG 1 cut(s) 763
PceI AGGCCT 1 cut(s) 427
PctI GAATGC 2 cut(s) 1128, 1254
PfeI GAWTC 5 cut(s) 71, 1177, 1285, 1356, 1468
PfoI TCCNGGA 1 cut(s) 165
PkrI GCNGC 4 cut(s) 7, 10, 1042, 1069
PleI GAGTC 1 cut(s) 566
PpsI GAGTC 1 cut(s) 566
PpuMI RGGWCCY 1 cut(s) 1325
Psp5II RGGWCCY 1 cut(s) 1325
Psp6I CCWGG 2 cut(s) 762, 972
PspFI CCCAGC 2 cut(s) 34, 1373
PspGI CCWGG 2 cut(s) 762, 972
PspN4I GGNNCC 5 cut(s) 928, 1326, 1327, 1420, 1554
PspPI GGNCC 5 cut(s) 131, 323, 488, 521, 1325
PspPPI RGGWCCY 1 cut(s) 1325
PspXI VCTCGAGB 1 cut(s) 920
PstNI CAGNNNCTG 2 cut(s) 11, 1139
PsuI RGATCY 3 cut(s) 407, 593, 901
PvuII CAGCTG 1 cut(s) 11
RsaI GTAC 5 cut(s) 232, 780, 880, 1199, 1432
RsaNI GTAC 5 cut(s) 231, 779, 879, 1198, 1431
SaqAI TTAA 6 cut(s) 83, 341, 362, 546, 731, 857
SatI GCNGC 4 cut(s) 6, 9, 1041, 1068
Sau3AI GATC 8 cut(s) 101, 265, 407, 445, 593, 753, 840, 901
Sau96I GGNCC 5 cut(s) 131, 323, 488, 521, 1325
ScaI AGTACT 1 cut(s) 880
SchI GAGTC 1 cut(s) 566
ScrFI CCNGG 5 cut(s) 167, 764, 783, 974, 1529
SduI GDGCHC 1 cut(s) 1423
SfaNI GCATC 3 cut(s) 189, 1126, 1312
SfcI CTRYAG 1 cut(s) 1296
Sfr274I CTCGAG 1 cut(s) 920
SfuI TTCGAA 1 cut(s) 1150
SinI GGWCC 3 cut(s) 488, 521, 1325
SlaI CTCGAG 1 cut(s) 920
SmlI CTYRAG 2 cut(s) 188, 920
SmoI CTYRAG 2 cut(s) 188, 920
SseBI AGGCCT 1 cut(s) 427
SspI AATATT 2 cut(s) 726, 1164
SspMI CTAG 1 cut(s) 830
StuI AGGCCT 1 cut(s) 427
StyD4I CCNGG 5 cut(s) 165, 762, 781, 972, 1527
StyI CCWWGG 2 cut(s) 466, 1230
TaaI ACNGT 3 cut(s) 22, 459, 1213
TaiI ACGT 2 cut(s) 692, 1541
TaqI TCGA 5 cut(s) 612, 921, 1150, 1306, 1359
TatI WGTACW 3 cut(s) 878, 1197, 1430
TfiI GAWTC 5 cut(s) 71, 1177, 1285, 1356, 1468
Tru1I TTAA 6 cut(s) 83, 341, 362, 546, 731, 857
Tru9I TTAA 6 cut(s) 83, 341, 362, 546, 731, 857
TscAI CASTG 6 cut(s) 36, 241, 462, 850, 1036, 1194
TseFI GTSAC 2 cut(s) 893, 1473
TseI GCWGC 4 cut(s) 5, 8, 1040, 1067
Tsp45I GTSAC 2 cut(s) 893, 1473
TspDTI ATGAA 6 cut(s) 84, 132, 188, 954, 1172, 1353
TspGWI ACGGA 2 cut(s) 233, 1085
TspRI CASTG 6 cut(s) 36, 241, 462, 850, 1036, 1194
VpaK11BI GGWCC 3 cut(s) 488, 521, 1325
XapI RAATTY 2 cut(s) 1112, 1340
XceI RCATGY 1 cut(s) 257
XcmI CCANNNNNNNNNTGG 1 cut(s) 770
XhoI CTCGAG 1 cut(s) 920
XspI CTAG 1 cut(s) 830
ZrmI AGTACT 1 cut(s) 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.