RLG00000028864

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005196
Physical Location & Seq
Reverse (-)
29483927 .. 29484453
527 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028864

Sequence Viewer

Length: 366 bp
ATGATCCTTGAGGGAGCCAAAAACTATGGTGACACAGATAGTCTATTTTCAGGTATCTCCAAGGGTAATTTCATAGTGGATAATTGCAAGAGTATATACTTTACTGGCTATGAGACCACTGCAATAACAACATCATGGAGCTTGATGTTATTGGCTGCAGATCCTGAGTGGCAAGCTCGTGCTGAGGTGCTTGAAATTTGCCGAGTTGCTGTTCCCGATGCTGAAATGCTTCGAAACATGAAAACATTCAATATGGTGATTCAAGAAACATTGCACCTCTATCCCCCAGCAACATATGTCATAAGGCAAGCCATGGAAGATATTAAATTCAAAGATATTGTAATCCCAAAAGGAATGAATCTTTAG

Protein Analysis

122

Amino Acids

13.67

Weight (kDa)

4.89

Isoelectric Point (pI)

13.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 25 - 120 1.7e-19 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 155
AcsI RAATTY 2 cut(s) 195, 326
AgsI TTSAA 4 cut(s) 194, 250, 263, 331
AjuI GAANNNNNNNTTGG 2 cut(s) 53, 85
AluBI AGCT 2 cut(s) 141, 176
AluI AGCT 2 cut(s) 141, 176
Alw26I GTCTC 1 cut(s) 107
AlwI GGATC 1 cut(s) 155
AlwNI CAGNNNCTG 1 cut(s) 164
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 2 cut(s) 195, 326
Asp700I GAANNNNTTC 2 cut(s) 228, 245
AsuHPI GGTGA 2 cut(s) 41, 268
AsuII TTCGAA 1 cut(s) 232
BauI CACGAG 1 cut(s) 177
BbvCI CCTCAGC 1 cut(s) 183
BbvI GCAGC 1 cut(s) 142
BcoDI GTCTC 1 cut(s) 107
BfmI CTRYAG 1 cut(s) 156
BisI GCNGC 1 cut(s) 156
BlsI GCNGC 1 cut(s) 157
BmiI GGNNCC 1 cut(s) 16
BmsI GCATC 1 cut(s) 208
Bpu10I CCTNAGC 1 cut(s) 183
Bpu14I TTCGAA 1 cut(s) 232
BpuEI CTTGAG 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 2 cut(s) 60, 312
Bse1I ACTGG 1 cut(s) 109
Bse3DI GCAATG 1 cut(s) 269
BseDI CCNNGG 2 cut(s) 60, 312
BseMI GCAATG 1 cut(s) 269
BseMII CTCAG 2 cut(s) 156, 174
BseNI ACTGG 1 cut(s) 109
BseXI GCAGC 1 cut(s) 142
BseYI CCCAGC 1 cut(s) 286
BsmAI GTCTC 1 cut(s) 107
Bso31I GGTCTC 1 cut(s) 107
Bsp119I TTCGAA 1 cut(s) 232
Bsp143I GATC 2 cut(s) 3, 160
Bsp19I CCATGG 1 cut(s) 312
BspCNI CTCAG 2 cut(s) 157, 175
BspLI GGNNCC 1 cut(s) 16
BspMAI CTGCAG 1 cut(s) 160
BspPI GGATC 1 cut(s) 155
BspT104I TTCGAA 1 cut(s) 232
BspTNI GGTCTC 1 cut(s) 107
BsrDI GCAATG 1 cut(s) 269
BsrI ACTGG 1 cut(s) 109
BssECI CCNNGG 2 cut(s) 60, 312
BssMI GATC 2 cut(s) 3, 160
BssSI CACGAG 1 cut(s) 177
BssT1I CCWWGG 2 cut(s) 60, 312
Bst2BI CACGAG 1 cut(s) 177
BstBI TTCGAA 1 cut(s) 232
BstC8I GCNNGC 2 cut(s) 174, 309
BstDEI CTNAG 2 cut(s) 165, 183
BstDSI CCRYGG 1 cut(s) 312
BstKTI GATC 2 cut(s) 6, 163
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 2 cut(s) 3, 160
BstSFI CTRYAG 1 cut(s) 156
BstV1I GCAGC 1 cut(s) 142
BstX2I RGATCY 1 cut(s) 160
BstYI RGATCY 1 cut(s) 160
BtgI CCRYGG 1 cut(s) 312
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 1 cut(s) 117
Cac8I GCNNGC 2 cut(s) 174, 309
CaiI CAGNNNCTG 1 cut(s) 164
CviAII CATG 3 cut(s) 135, 238, 313
CviJI RGCY 6 cut(s) 17, 108, 141, 155, 176, 311
CviKI_1 RGCY 6 cut(s) 17, 108, 141, 155, 176, 311
DdeI CTNAG 2 cut(s) 165, 183
DpnI GATC 2 cut(s) 5, 162
DpnII GATC 2 cut(s) 3, 160
Eco130I CCWWGG 2 cut(s) 60, 312
Eco31I GGTCTC 1 cut(s) 107
EcoT14I CCWWGG 2 cut(s) 60, 312
ErhI CCWWGG 2 cut(s) 60, 312
FaeI CATG 3 cut(s) 138, 241, 316
FatI CATG 3 cut(s) 134, 237, 312
FauNDI CATATG 1 cut(s) 295
Fnu4HI GCNGC 1 cut(s) 156
Fsp4HI GCNGC 1 cut(s) 156
GluI GCNGC 1 cut(s) 156
GsaI CCCAGC 1 cut(s) 290
Hin1II CATG 3 cut(s) 138, 241, 316
HinfI GANTC 2 cut(s) 259, 358
HphI GGTGA 2 cut(s) 41, 268
Hpy188III TCNNGA 3 cut(s) 164, 215, 263
HpyCH4V TGCA 4 cut(s) 87, 122, 158, 274
HpyF3I CTNAG 2 cut(s) 165, 183
Hsp92II CATG 3 cut(s) 138, 241, 316
Kzo9I GATC 2 cut(s) 3, 160
LmnI GCTCC 2 cut(s) 14, 138
LpnPI CCDG 4 cut(s) 36, 90, 177, 300
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 1 cut(s) 208
MaeIII GTNAC 1 cut(s) 29
MalI GATC 2 cut(s) 5, 162
MboI GATC 2 cut(s) 3, 160
MboII GAAGA 1 cut(s) 329
MflI RGATCY 1 cut(s) 160
MluCI AATT 4 cut(s) 67, 82, 195, 326
MnlI CCTC 3 cut(s) 4, 178, 287
MroXI GAANNNNTTC 2 cut(s) 228, 245
MseI TTAA 1 cut(s) 324
NcoI CCATGG 1 cut(s) 312
NdeI CATATG 1 cut(s) 295
NdeII GATC 2 cut(s) 3, 160
NlaIII CATG 3 cut(s) 138, 241, 316
NlaIV GGNNCC 1 cut(s) 16
NmeAIII GCCGAG 1 cut(s) 227
NmuCI GTSAC 1 cut(s) 29
NspV TTCGAA 1 cut(s) 232
PdmI GAANNNNTTC 2 cut(s) 228, 245
PfeI GAWTC 2 cut(s) 259, 358
PkrI GCNGC 1 cut(s) 157
PspFI CCCAGC 1 cut(s) 286
PspN4I GGNNCC 1 cut(s) 16
PstI CTGCAG 1 cut(s) 160
PstNI CAGNNNCTG 1 cut(s) 164
PsuI RGATCY 1 cut(s) 160
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 1 cut(s) 156
Sau3AI GATC 2 cut(s) 3, 160
SetI ASST 5 cut(s) 55, 143, 178, 189, 279
SfaNI GCATC 1 cut(s) 208
SfcI CTRYAG 1 cut(s) 156
SfuI TTCGAA 1 cut(s) 232
SmlI CTYRAG 1 cut(s) 8
SmoI CTYRAG 1 cut(s) 8
Sse9I AATT 4 cut(s) 67, 82, 195, 326
StyI CCWWGG 2 cut(s) 60, 312
TaqI TCGA 1 cut(s) 232
TasI AATT 4 cut(s) 67, 82, 195, 326
TfiI GAWTC 2 cut(s) 259, 358
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 1 cut(s) 29
TseI GCWGC 1 cut(s) 155
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 2 cut(s) 61, 254
TspRI CASTG 1 cut(s) 124
XapI RAATTY 2 cut(s) 195, 326
XmnI GAANNNNTTC 2 cut(s) 228, 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.