FvH4_7g15460

Desiccation protectant protein Lea14 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003820
Physical Location & Seq
Forward (+)
13397980 .. 13400435
2456 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g15460.t1

Sequence Viewer

Length: 459 bp
ATGGCTGGGATATTTGACAAGGCTAAGAACTTCGCGACTGAGAAACTGGGCAACATAGAAAAGCCAGAGGCAGAGCTGACAGATGTTGACTTCAGGAAGGCGAGCTTCGATGCTGCGGAGTACCTCGGCAAGGTTCATGTCAAGAACCCTTATGATCACTCCATCCCCATCTGCGAGATCTCCTACACCCTCAAATGTGTTGGCAGGGAGATTGTAGCTGGGACAGTTCCGGACCCTGGATCGATTAAGGCAAGCGATGTTACCATTCTGGAGGTACCAGTGAAGGTGCCACATAGTGTGTTGGCGACCTTGTGTAAGGATGTTGGGGCAGATTGGGACATTGATTATGAGTTGATATTGGGTCTCACCATTGACCTTCCTGTGATTGGGAACTTCACCATACCTCTGTCCAAGAAGGGAGAGATCAAGCTTCCCAGTCTGACCAGCCTCTTTGGTTGA

Protein Analysis

153

Amino Acids

16.47

Weight (kDa)

5.02

Isoelectric Point (pI)

19.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 44 - 140 2.1e-21 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 274
AccB1I GGYRCC 2 cut(s) 274, 286
AccII CGCG 1 cut(s) 35
AccIII TCCGGA 1 cut(s) 229
AciI CCGC 1 cut(s) 116
AclWI GGATC 1 cut(s) 247
AcuI CTGAAG 1 cut(s) 76
AdeI CACNNNGTG 1 cut(s) 296
AfaI GTAC 2 cut(s) 122, 276
AfiI CCNNNNNNNGG 3 cut(s) 130, 236, 386
AjnI CCWGG 1 cut(s) 235
AluBI AGCT 4 cut(s) 76, 105, 218, 430
AluI AGCT 4 cut(s) 76, 105, 218, 430
Alw26I GTCTC 1 cut(s) 368
AlwI GGATC 1 cut(s) 247
Aor13HI TCCGGA 1 cut(s) 229
ApeKI GCWGC 1 cut(s) 113
Asp718I GGTACC 1 cut(s) 274
AspS9I GGNCC 1 cut(s) 232
AsuHPI GGTGA 2 cut(s) 358, 388
AvaII GGWCC 1 cut(s) 232
BanI GGYRCC 2 cut(s) 274, 286
BbvI GCAGC 1 cut(s) 100
BccI CCATC 2 cut(s) 170, 176
BciT130I CCWGG 1 cut(s) 237
BclI TGATCA 1 cut(s) 154
BcoDI GTCTC 1 cut(s) 368
BglII AGATCT 1 cut(s) 177
BisI GCNGC 1 cut(s) 114
BlsI GCNGC 1 cut(s) 115
Bme1390I CCNGG 1 cut(s) 237
Bme18I GGWCC 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 3 cut(s) 234, 276, 288
BmrFI CCNGG 1 cut(s) 237
BmrI ACTGGG 2 cut(s) 56, 429
BmsI GCATC 1 cut(s) 100
BmuI ACTGGG 2 cut(s) 56, 429
BpmI CTGGAG 1 cut(s) 290
Bsa29I ATCGAT 1 cut(s) 242
BsaI GGTCTC 1 cut(s) 368
BsaJI CCNNGG 2 cut(s) 124, 235
BsaWI WCCGGW 1 cut(s) 229
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 236, 386
Bse1I ACTGG 3 cut(s) 51, 278, 435
BseAI TCCGGA 1 cut(s) 229
BseBI CCWGG 1 cut(s) 237
BseCI ATCGAT 1 cut(s) 242
BseDI CCNNGG 2 cut(s) 124, 235
BseGI GGATG 2 cut(s) 162, 325
BseLI CCNNNNNNNGG 3 cut(s) 130, 236, 386
BseMII CTCAG 1 cut(s) 30
BseNI ACTGG 3 cut(s) 51, 278, 435
BseXI GCAGC 1 cut(s) 100
BseYI CCCAGC 2 cut(s) 5, 218
Bsh1236I CGCG 1 cut(s) 35
BshNI GGYRCC 2 cut(s) 274, 286
BshVI ATCGAT 1 cut(s) 242
BsiSI CCGG 1 cut(s) 230
BslFI GGGAC 2 cut(s) 235, 350
BslI CCNNNNNNNGG 3 cut(s) 130, 236, 386
BsmAI GTCTC 1 cut(s) 368
BsmFI GGGAC 2 cut(s) 235, 350
Bso31I GGTCTC 1 cut(s) 368
Bsp13I TCCGGA 1 cut(s) 229
Bsp143I GATC 4 cut(s) 154, 177, 239, 423
Bsp68I TCGCGA 1 cut(s) 35
BspACI CCGC 1 cut(s) 116
BspCNI CTCAG 1 cut(s) 31
BspDI ATCGAT 1 cut(s) 242
BspEI TCCGGA 1 cut(s) 229
BspFNI CGCG 1 cut(s) 35
BspLI GGNNCC 3 cut(s) 234, 276, 288
BspPI GGATC 1 cut(s) 247
BspT107I GGYRCC 2 cut(s) 274, 286
BspTNI GGTCTC 1 cut(s) 368
BsrI ACTGG 3 cut(s) 51, 278, 435
BssECI CCNNGG 2 cut(s) 124, 235
BssMI GATC 4 cut(s) 154, 177, 239, 423
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 226
BstC8I GCNNGC 2 cut(s) 103, 253
BstDEI CTNAG 2 cut(s) 24, 39
BstENI CCTNNNNNAGG 1 cut(s) 128
BstF5I GGATG 2 cut(s) 162, 325
BstFNI CGCG 1 cut(s) 35
BstKTI GATC 4 cut(s) 157, 180, 242, 426
BstMAI GTCTC 1 cut(s) 368
BstMBI GATC 4 cut(s) 154, 177, 239, 423
BstNI CCWGG 1 cut(s) 237
BstSCI CCNGG 1 cut(s) 235
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 1 cut(s) 100
BstX2I RGATCY 1 cut(s) 177
BstYI RGATCY 1 cut(s) 177
Bsu15I ATCGAT 1 cut(s) 242
BsuTUI ATCGAT 1 cut(s) 242
BtgZI GCGATG 1 cut(s) 270
BtsCI GGATG 2 cut(s) 162, 325
BtsIMutI CAGTG 1 cut(s) 285
BtuMI TCGCGA 1 cut(s) 35
Cac8I GCNNGC 2 cut(s) 103, 253
Cfr13I GGNCC 1 cut(s) 232
ClaI ATCGAT 1 cut(s) 242
Csp6I GTAC 2 cut(s) 121, 275
CviAII CATG 1 cut(s) 137
CviJI RGCY 8 cut(s) 5, 23, 64, 76, 105, 218, 430, 447
CviKI_1 RGCY 8 cut(s) 5, 23, 64, 76, 105, 218, 430, 447
CviQI GTAC 2 cut(s) 121, 275
DdeI CTNAG 2 cut(s) 24, 39
DpnI GATC 4 cut(s) 156, 179, 241, 425
DpnII GATC 4 cut(s) 154, 177, 239, 423
DraIII CACNNNGTG 1 cut(s) 296
Eco31I GGTCTC 1 cut(s) 368
Eco47I GGWCC 1 cut(s) 232
Eco57I CTGAAG 1 cut(s) 76
EcoNI CCTNNNNNAGG 1 cut(s) 128
EcoRII CCWGG 1 cut(s) 235
FaeI CATG 1 cut(s) 140
FaiI YATR 6 cut(s) 56, 138, 153, 294, 348, 401
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FaqI GGGAC 2 cut(s) 235, 350
FatI CATG 1 cut(s) 136
FbaI TGATCA 1 cut(s) 154
Fnu4HI GCNGC 1 cut(s) 114
FokI GGATG 2 cut(s) 149, 332
Fsp4HI GCNGC 1 cut(s) 114
GluI GCNGC 1 cut(s) 114
GsaI CCCAGC 2 cut(s) 9, 222
GsuI CTGGAG 1 cut(s) 290
HapII CCGG 1 cut(s) 230
Hin1II CATG 1 cut(s) 140
HincII GTYRAC 1 cut(s) 88
HindII GTYRAC 1 cut(s) 88
HindIII AAGCTT 1 cut(s) 428
HpaII CCGG 1 cut(s) 230
HphI GGTGA 2 cut(s) 358, 388
Hpy166II GTNNAC 1 cut(s) 88
Hpy188I TCNGA 1 cut(s) 441
Hpy188III TCNNGA 5 cut(s) 34, 94, 142, 230, 269
Hpy8I GTNNAC 1 cut(s) 88
HpyAV CCTTC 4 cut(s) 91, 277, 386, 409
HpyCH4III ACNGT 1 cut(s) 226
HpyF3I CTNAG 2 cut(s) 24, 39
Hsp92II CATG 1 cut(s) 140
Kpn2I TCCGGA 1 cut(s) 229
KpnI GGTACC 1 cut(s) 278
Ksp22I TGATCA 1 cut(s) 154
Kzo9I GATC 4 cut(s) 154, 177, 239, 423
Lsp1109I GCAGC 1 cut(s) 100
LweI GCATC 1 cut(s) 100
MaeIII GTNAC 1 cut(s) 259
MalI GATC 4 cut(s) 156, 179, 241, 425
MboI GATC 4 cut(s) 154, 177, 239, 423
MflI RGATCY 1 cut(s) 177
MnlI CCTC 6 cut(s) 61, 134, 200, 265, 414, 458
MroI TCCGGA 1 cut(s) 229
MseI TTAA 1 cut(s) 246
MspI CCGG 1 cut(s) 230
MspR9I CCNGG 1 cut(s) 237
MvaI CCWGG 1 cut(s) 237
MvnI CGCG 1 cut(s) 35
NdeII GATC 4 cut(s) 154, 177, 239, 423
NlaIII CATG 1 cut(s) 140
NlaIV GGNNCC 3 cut(s) 234, 276, 288
NmeAIII GCCGAG 1 cut(s) 105
NruI TCGCGA 1 cut(s) 35
PkrI GCNGC 1 cut(s) 115
Psp6I CCWGG 1 cut(s) 235
PspFI CCCAGC 2 cut(s) 5, 218
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 3 cut(s) 234, 276, 288
PspPI GGNCC 1 cut(s) 232
PsuI RGATCY 1 cut(s) 177
RruI TCGCGA 1 cut(s) 35
RsaI GTAC 2 cut(s) 122, 276
RsaNI GTAC 2 cut(s) 121, 275
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 1 cut(s) 114
Sau3AI GATC 4 cut(s) 154, 177, 239, 423
Sau96I GGNCC 1 cut(s) 232
ScrFI CCNGG 1 cut(s) 237
SfaNI GCATC 1 cut(s) 100
SinI GGWCC 1 cut(s) 232
SsiI CCGC 1 cut(s) 116
StyD4I CCNGG 1 cut(s) 235
TaaI ACNGT 1 cut(s) 226
TaqI TCGA 2 cut(s) 108, 242
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 1 cut(s) 285
TseI GCWGC 1 cut(s) 113
TspDTI ATGAA 1 cut(s) 125
TspRI CASTG 1 cut(s) 285
VpaK11BI GGWCC 1 cut(s) 232
XagI CCTNNNNNAGG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.