RLG00000027999

Desiccation protectant protein Lea14 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003820
Physical Location & Seq
Reverse (-)
17585742 .. 17587827
2086 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027999

Sequence Viewer

Length: 735 bp
ATGTTGAAGTCGACGGAGGATGTGGAGAAGGAGGTTAATAAGGAGGAGGACAAAACGGAGAGGTCGCAGATGAAAATGGTTAAGTCGACGGAGGAGGTTAAGGAGGAGGCGGATGAGGGTAAGAGGGACAGGTCTTTGATGGGAATGTTTAAATCGAAGGAGGAGGCGAAGAAGGAGGTTGAGGAGGAGGCGAAGGAGGCTAAGACGGAGGAGTCGTTGATAGACAAGGCGAAGAACTATGTGGCAGAGAAGATAGCAAATGTGCCAACGCCAGAGGCAAGTCTGGTCGATGTTGATTTCAAGAAGGTGAGCTTTGATTCTGCCGATTACCTTGCCAAGGTTAACATCAAAAATCCCTATTCACATTCCATCCCCATATGTGAGATCGATTACACCCTCAAAAGCATTGGCAGTGTGATCGCTTCCGGGAGAATTCCAGACCCTGGATCGATAAAGGCAAGCTGTGATACTCAGGTGGATGTACCAGTGAAGGTGCCACACAGTATAATAGTGACTTTGGTTAAGGACATCGCTACAGATTGGGACATTGACTACGAGTTGGAACTGGGTCTCATCATTGACCTTCCTGTAATTGGAAACATTACCATACCACTATCCAGGAAAGGTGAGATCAAGCTTCCCACCTTAAAGGAGTTAGTATTTGGAGCAGAGGAGGAGGATTCAAAAGAAAAAGACAAGGATTCAAAGGAAAAAGATAAGGATTCAGATAAATAG

Protein Analysis

245

Amino Acids

27.31

Weight (kDa)

4.78

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 113 - 209 1.6e-21 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 211
AccB1I GGYRCC 1 cut(s) 493
AccB7I CCANNNNNTGG 1 cut(s) 443
AccI GTMKAC 2 cut(s) 11, 86
AciI CCGC 1 cut(s) 110
AclWI GGATC 1 cut(s) 454
AcsI RAATTY 1 cut(s) 432
AfaI GTAC 1 cut(s) 483
AfiI CCNNNNNNNGG 3 cut(s) 337, 443, 593
AgsI TTSAA 4 cut(s) 7, 301, 684, 705
AjnI CCWGG 2 cut(s) 442, 617
AluBI AGCT 3 cut(s) 312, 462, 637
AluI AGCT 3 cut(s) 312, 462, 637
Alw26I GTCTC 1 cut(s) 575
AlwI GGATC 1 cut(s) 454
AlwNI CAGNNNCTG 1 cut(s) 443
ApoI RAATTY 1 cut(s) 432
AsuC2I CCSGG 1 cut(s) 427
AsuHPI GGTGA 2 cut(s) 319, 638
BaeI ACNNNNGTAYC 2 cut(s) 459, 492
BanI GGYRCC 1 cut(s) 493
BccI CCATC 2 cut(s) 133, 377
BcgI CGANNNNNNTGC 4 cut(s) 314, 348, 400, 434
BciT130I CCWGG 2 cut(s) 444, 619
BcnI CCSGG 1 cut(s) 427
BcoDI GTCTC 1 cut(s) 575
BfmI CTRYAG 1 cut(s) 534
Bme1390I CCNGG 3 cut(s) 427, 444, 619
BmiI GGNNCC 1 cut(s) 495
BmrFI CCNGG 3 cut(s) 427, 444, 619
BmrI ACTGGG 1 cut(s) 575
BmuI ACTGGG 1 cut(s) 575
BpuMI CCSGG 1 cut(s) 427
Bsa29I ATCGAT 2 cut(s) 387, 449
BsaI GGTCTC 1 cut(s) 575
BsaJI CCNNGG 2 cut(s) 336, 442
BsaXI ACNNNNNCTCC 2 cut(s) 200, 230
Bsc4I CCNNNNNNNGG 3 cut(s) 337, 443, 593
Bse1I ACTGG 2 cut(s) 485, 570
BseBI CCWGG 2 cut(s) 444, 619
BseCI ATCGAT 2 cut(s) 387, 449
BseDI CCNNGG 2 cut(s) 336, 442
BseGI GGATG 4 cut(s) 25, 118, 369, 484
BseLI CCNNNNNNNGG 3 cut(s) 337, 443, 593
BseMII CTCAG 1 cut(s) 485
BseNI ACTGG 2 cut(s) 485, 570
BseRI GAGGAG 9 cut(s) 59, 107, 119, 176, 197, 200, 224, 686, 689
BshNI GGYRCC 1 cut(s) 493
BshVI ATCGAT 2 cut(s) 387, 449
BsiSI CCGG 1 cut(s) 426
BslFI GGGAC 2 cut(s) 140, 557
BslI CCNNNNNNNGG 3 cut(s) 337, 443, 593
BsmAI GTCTC 1 cut(s) 575
BsmFI GGGAC 2 cut(s) 140, 557
Bso31I GGTCTC 1 cut(s) 575
Bsp143I GATC 4 cut(s) 384, 417, 446, 630
BspACI CCGC 1 cut(s) 110
BspCNI CTCAG 1 cut(s) 484
BspDI ATCGAT 2 cut(s) 387, 449
BspLI GGNNCC 1 cut(s) 495
BspPI GGATC 1 cut(s) 454
BspT107I GGYRCC 1 cut(s) 493
BspTNI GGTCTC 1 cut(s) 575
BsrI ACTGG 2 cut(s) 485, 570
BssECI CCNNGG 2 cut(s) 336, 442
BssMI GATC 4 cut(s) 384, 417, 446, 630
BssT1I CCWWGG 1 cut(s) 336
Bst2UI CCWGG 2 cut(s) 444, 619
Bst4CI ACNGT 1 cut(s) 503
BstC8I GCNNGC 1 cut(s) 460
BstDEI CTNAG 2 cut(s) 201, 471
BstENI CCTNNNNNAGG 1 cut(s) 335
BstF5I GGATG 4 cut(s) 25, 118, 369, 484
BstKTI GATC 4 cut(s) 387, 420, 449, 633
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 4 cut(s) 384, 417, 446, 630
BstMWI GCNNNNNNNGC 1 cut(s) 197
BstNI CCWGG 2 cut(s) 444, 619
BstSCI CCNGG 3 cut(s) 425, 442, 617
BstSFI CTRYAG 1 cut(s) 534
Bsu15I ATCGAT 2 cut(s) 387, 449
BsuTUI ATCGAT 2 cut(s) 387, 449
BtgZI GCGATG 1 cut(s) 514
BtsCI GGATG 4 cut(s) 25, 118, 369, 484
BtsI GCAGTG 1 cut(s) 418
BtsIMutI CAGTG 2 cut(s) 418, 492
Cac8I GCNNGC 1 cut(s) 460
CaiI CAGNNNCTG 1 cut(s) 443
ClaI ATCGAT 2 cut(s) 387, 449
Csp6I GTAC 1 cut(s) 482
CviJI RGCY 4 cut(s) 200, 312, 462, 637
CviKI_1 RGCY 4 cut(s) 200, 312, 462, 637
CviQI GTAC 1 cut(s) 482
DdeI CTNAG 2 cut(s) 201, 471
DpnI GATC 4 cut(s) 386, 419, 448, 632
DpnII GATC 4 cut(s) 384, 417, 446, 630
DraI TTTAAA 1 cut(s) 151
DrdI GACNNNNNNGTC 1 cut(s) 211
DseDI GACNNNNNNGTC 1 cut(s) 211
EciI GGCGGA 1 cut(s) 125
Eco130I CCWWGG 1 cut(s) 336
Eco31I GGTCTC 1 cut(s) 575
EcoNI CCTNNNNNAGG 1 cut(s) 335
EcoRI GAATTC 1 cut(s) 432
EcoRII CCWGG 2 cut(s) 442, 617
EcoT14I CCWWGG 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 336
FaiI YATR 5 cut(s) 240, 377, 379, 506, 608
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FaqI GGGAC 2 cut(s) 140, 557
FauNDI CATATG 1 cut(s) 377
FblI GTMKAC 2 cut(s) 11, 86
FokI GGATG 4 cut(s) 32, 125, 356, 491
HapII CCGG 1 cut(s) 426
HincII GTYRAC 3 cut(s) 12, 87, 343
HindII GTYRAC 3 cut(s) 12, 87, 343
HindIII AAGCTT 1 cut(s) 635
HinfI GANTC 5 cut(s) 212, 317, 680, 701, 722
HpaI GTTAAC 1 cut(s) 343
HpaII CCGG 1 cut(s) 426
HphI GGTGA 2 cut(s) 319, 638
Hpy166II GTNNAC 3 cut(s) 12, 87, 343
Hpy188I TCNGA 1 cut(s) 727
Hpy188III TCNNGA 2 cut(s) 301, 437
Hpy8I GTNNAC 3 cut(s) 12, 87, 343
Hpy99I CGWCG 2 cut(s) 16, 91
HpyAV CCTTC 7 cut(s) 22, 151, 166, 187, 298, 484, 593
HpyCH4III ACNGT 1 cut(s) 503
HpyF10VI GCNNNNNNNGC 1 cut(s) 197
HpyF3I CTNAG 2 cut(s) 201, 471
KspAI GTTAAC 1 cut(s) 343
Kzo9I GATC 4 cut(s) 384, 417, 446, 630
LmnI GCTCC 1 cut(s) 665
MaeIII GTNAC 1 cut(s) 511
MalI GATC 4 cut(s) 386, 419, 448, 632
MboI GATC 4 cut(s) 384, 417, 446, 630
MboII GAAGA 3 cut(s) 181, 244, 262
MluCI AATT 2 cut(s) 432, 591
MlyI GAGTC 1 cut(s) 221
MmeI TCCRAC 1 cut(s) 540
MseI TTAA 7 cut(s) 36, 81, 99, 150, 342, 522, 647
MspI CCGG 1 cut(s) 426
MspR9I CCNGG 3 cut(s) 427, 444, 619
MvaI CCWGG 2 cut(s) 444, 619
MwoI GCNNNNNNNGC 1 cut(s) 197
NciI CCSGG 1 cut(s) 427
NdeI CATATG 1 cut(s) 377
NdeII GATC 4 cut(s) 384, 417, 446, 630
NlaIV GGNNCC 1 cut(s) 495
NmuCI GTSAC 1 cut(s) 511
PcsI WCGNNNNNNNCGW 1 cut(s) 212
PfeI GAWTC 4 cut(s) 317, 680, 701, 722
PflMI CCANNNNNTGG 1 cut(s) 443
PfoI TCCNGGA 2 cut(s) 425, 617
PleI GAGTC 1 cut(s) 220
PpsI GAGTC 1 cut(s) 220
Psp6I CCWGG 2 cut(s) 442, 617
PspGI CCWGG 2 cut(s) 442, 617
PspN4I GGNNCC 1 cut(s) 495
PstNI CAGNNNCTG 1 cut(s) 443
RsaI GTAC 1 cut(s) 483
RsaNI GTAC 1 cut(s) 482
SalI GTCGAC 2 cut(s) 10, 85
SaqAI TTAA 7 cut(s) 36, 81, 99, 150, 342, 522, 647
Sau3AI GATC 4 cut(s) 384, 417, 446, 630
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 3 cut(s) 427, 444, 619
SfcI CTRYAG 1 cut(s) 534
Sse9I AATT 2 cut(s) 432, 591
SsiI CCGC 1 cut(s) 110
StyD4I CCNGG 3 cut(s) 425, 442, 617
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 1 cut(s) 503
TaqI TCGA 6 cut(s) 11, 86, 155, 288, 387, 449
TasI AATT 2 cut(s) 432, 591
TfiI GAWTC 4 cut(s) 317, 680, 701, 722
Tru1I TTAA 7 cut(s) 36, 81, 99, 150, 342, 522, 647
Tru9I TTAA 7 cut(s) 36, 81, 99, 150, 342, 522, 647
TscAI CASTG 2 cut(s) 418, 492
TseFI GTSAC 1 cut(s) 511
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 1 cut(s) 86
TspGWI ACGGA 4 cut(s) 29, 71, 104, 221
TspRI CASTG 2 cut(s) 418, 492
Van91I CCANNNNNTGG 1 cut(s) 443
XagI CCTNNNNNAGG 1 cut(s) 335
XapI RAATTY 1 cut(s) 432
XmiI GTMKAC 2 cut(s) 11, 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.