RchiOBHm_Chr1g0357761

Desiccation protectant protein Lea14 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003820
Physical Location & Seq
Forward (+)
50058916 .. 50060807
1892 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58298

Sequence Viewer

Length: 774 bp
ATGTTGAAGTCGACGGAGGATGTGGAGAAGGAGGTTAATAAGGAGGAGGACAAAACGGAGAGGTCGCTGATGAAAATGGCTAAGACGACGGAGGAGACAGCGGAGGAGGTTAAGGAGGAGGGGGATGAGGGTAAGAGGGACAGGTCTTTGATGGGAATGTTTAAATCGAAGGAGGAGGCGAAGAAGGAGGTTGAGGAGGAGGCGAAGGAGGCTAAGACGGAGGAGTCGTTGATAGACAAGGCGAAGAACTATGTGGCAGAGAAGATAGCAAATGTGCCAACGCCAGAGGCAAGTCTGGTCGATGTTGATTTCAAGAAGGTGAGCTTTAATTCTGCCGATTACCTTGCCAAGGTTAACATAAAAAATCCCTATTCACATTCCATCCCCATCTGTGAGATCGATTACACCCTCAAAAGCACTGGCAGTGTGATCGCTTCGGGGAGAATTCCAGACCCTGGATCGATAAAGGCTAGCGGTGATACTCAGGTGGATGTACCAGTGAAGGTGCCACACAGTATAATAGTGACTTTGGTTAAGGACATCGCTACAGATTGGGACATTGACTATGAGTTGGAACTGGGTCTCATCATTGACCTTCCTGTAATTGGGAACATTACCATACCACTATCCAGGAAAGGTGAGATCAAGCTTCCCACCTTAAAGGAGTTAGTATTTGGAGCAAAGGAGGAGGAGAAGGATTCAAAAGAAAAAGACATGGATTCAAAAGAAAAAGAGAAGGATTCAAAAGAAAAAGATAAGGATTCAGATAAATAG

Protein Analysis

257

Amino Acids

28.74

Weight (kDa)

4.83

Isoelectric Point (pI)

35.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 117 - 213 2.3e-23 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 223
AccB1I GGYRCC 1 cut(s) 505
AccB7I CCANNNNNTGG 1 cut(s) 455
AccI GTMKAC 1 cut(s) 11
AciI CCGC 2 cut(s) 101, 474
AclWI GGATC 1 cut(s) 466
AcsI RAATTY 1 cut(s) 444
AfaI GTAC 1 cut(s) 495
AfiI CCNNNNNNNGG 3 cut(s) 349, 455, 605
AgsI TTSAA 5 cut(s) 7, 313, 702, 723, 744
AjnI CCWGG 2 cut(s) 454, 629
AluBI AGCT 2 cut(s) 324, 649
AluI AGCT 2 cut(s) 324, 649
Alw26I GTCTC 2 cut(s) 89, 587
AlwI GGATC 1 cut(s) 466
AlwNI CAGNNNCTG 1 cut(s) 455
ApoI RAATTY 1 cut(s) 444
AsuHPI GGTGA 3 cut(s) 331, 488, 650
AsuNHI GCTAGC 1 cut(s) 470
BaeI ACNNNNGTAYC 2 cut(s) 471, 504
BanI GGYRCC 1 cut(s) 505
BccI CCATC 3 cut(s) 145, 389, 395
BcgI CGANNNNNNTGC 4 cut(s) 326, 360, 412, 446
BciT130I CCWGG 2 cut(s) 456, 631
BcoDI GTCTC 2 cut(s) 89, 587
BfaI CTAG 1 cut(s) 471
BfmI CTRYAG 1 cut(s) 546
Bme1390I CCNGG 2 cut(s) 456, 631
BmiI GGNNCC 1 cut(s) 507
BmrFI CCNGG 2 cut(s) 456, 631
BmrI ACTGGG 1 cut(s) 587
BmtI GCTAGC 1 cut(s) 474
BmuI ACTGGG 1 cut(s) 587
Bsa29I ATCGAT 2 cut(s) 399, 461
BsaI GGTCTC 1 cut(s) 587
BsaJI CCNNGG 2 cut(s) 348, 454
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 3 cut(s) 349, 455, 605
Bse1I ACTGG 3 cut(s) 424, 497, 582
BseBI CCWGG 2 cut(s) 456, 631
BseCI ATCGAT 2 cut(s) 399, 461
BseDI CCNNGG 2 cut(s) 348, 454
BseGI GGATG 4 cut(s) 25, 130, 381, 496
BseLI CCNNNNNNNGG 3 cut(s) 349, 455, 605
BseMII CTCAG 1 cut(s) 497
BseNI ACTGG 3 cut(s) 424, 497, 582
BshNI GGYRCC 1 cut(s) 505
BshVI ATCGAT 2 cut(s) 399, 461
BslFI GGGAC 2 cut(s) 152, 569
BslI CCNNNNNNNGG 3 cut(s) 349, 455, 605
BsmAI GTCTC 2 cut(s) 89, 587
BsmFI GGGAC 2 cut(s) 152, 569
Bso31I GGTCTC 1 cut(s) 587
Bsp143I GATC 4 cut(s) 396, 429, 458, 642
BspACI CCGC 2 cut(s) 101, 474
BspCNI CTCAG 1 cut(s) 496
BspDI ATCGAT 2 cut(s) 399, 461
BspLI GGNNCC 1 cut(s) 507
BspOI GCTAGC 1 cut(s) 474
BspPI GGATC 1 cut(s) 466
BspT107I GGYRCC 1 cut(s) 505
BspTNI GGTCTC 1 cut(s) 587
BsrI ACTGG 3 cut(s) 424, 497, 582
BssECI CCNNGG 2 cut(s) 348, 454
BssMI GATC 4 cut(s) 396, 429, 458, 642
BssT1I CCWWGG 1 cut(s) 348
Bst2UI CCWGG 2 cut(s) 456, 631
Bst4CI ACNGT 1 cut(s) 515
BstC8I GCNNGC 1 cut(s) 472
BstDEI CTNAG 3 cut(s) 81, 213, 483
BstENI CCTNNNNNAGG 1 cut(s) 347
BstF5I GGATG 4 cut(s) 25, 130, 381, 496
BstKTI GATC 4 cut(s) 399, 432, 461, 645
BstMAI GTCTC 2 cut(s) 89, 587
BstMBI GATC 4 cut(s) 396, 429, 458, 642
BstMWI GCNNNNNNNGC 1 cut(s) 209
BstNI CCWGG 2 cut(s) 456, 631
BstSCI CCNGG 2 cut(s) 454, 629
BstSFI CTRYAG 1 cut(s) 546
Bsu15I ATCGAT 2 cut(s) 399, 461
BsuTUI ATCGAT 2 cut(s) 399, 461
BtgZI GCGATG 1 cut(s) 526
BtsCI GGATG 4 cut(s) 25, 130, 381, 496
BtsI GCAGTG 1 cut(s) 430
BtsIMutI CAGTG 3 cut(s) 417, 430, 504
Cac8I GCNNGC 1 cut(s) 472
CaiI CAGNNNCTG 1 cut(s) 455
ClaI ATCGAT 2 cut(s) 399, 461
Csp6I GTAC 1 cut(s) 494
CviAII CATG 1 cut(s) 715
CviJI RGCY 5 cut(s) 80, 212, 324, 470, 649
CviKI_1 RGCY 5 cut(s) 80, 212, 324, 470, 649
CviQI GTAC 1 cut(s) 494
DdeI CTNAG 3 cut(s) 81, 213, 483
DpnI GATC 4 cut(s) 398, 431, 460, 644
DpnII GATC 4 cut(s) 396, 429, 458, 642
DraI TTTAAA 1 cut(s) 163
DrdI GACNNNNNNGTC 1 cut(s) 223
DseDI GACNNNNNNGTC 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 348
Eco31I GGTCTC 1 cut(s) 587
EcoNI CCTNNNNNAGG 1 cut(s) 347
EcoRI GAATTC 1 cut(s) 444
EcoRII CCWGG 2 cut(s) 454, 629
EcoT14I CCWWGG 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 1 cut(s) 718
FaiI YATR 6 cut(s) 252, 359, 518, 567, 620, 716
FalI AAGNNNNNCTT 2 cut(s) 308, 340
FaqI GGGAC 2 cut(s) 152, 569
FatI CATG 1 cut(s) 714
FblI GTMKAC 1 cut(s) 11
FokI GGATG 4 cut(s) 32, 137, 368, 503
FspBI CTAG 1 cut(s) 471
Hin1II CATG 1 cut(s) 718
HincII GTYRAC 2 cut(s) 12, 355
HindII GTYRAC 2 cut(s) 12, 355
HindIII AAGCTT 1 cut(s) 647
HinfI GANTC 5 cut(s) 224, 698, 719, 740, 761
HpaI GTTAAC 1 cut(s) 355
HphI GGTGA 3 cut(s) 331, 488, 650
Hpy166II GTNNAC 2 cut(s) 12, 355
Hpy188I TCNGA 1 cut(s) 766
Hpy188III TCNNGA 2 cut(s) 313, 449
Hpy8I GTNNAC 2 cut(s) 12, 355
Hpy99I CGWCG 2 cut(s) 16, 91
HpyAV CCTTC 9 cut(s) 22, 163, 178, 199, 310, 496, 605, 688, 730
HpyCH4III ACNGT 1 cut(s) 515
HpyF10VI GCNNNNNNNGC 1 cut(s) 209
HpyF3I CTNAG 3 cut(s) 81, 213, 483
Hsp92II CATG 1 cut(s) 718
KspAI GTTAAC 1 cut(s) 355
Kzo9I GATC 4 cut(s) 396, 429, 458, 642
LmnI GCTCC 1 cut(s) 677
MaeI CTAG 1 cut(s) 471
MaeIII GTNAC 1 cut(s) 523
MalI GATC 4 cut(s) 398, 431, 460, 644
MboI GATC 4 cut(s) 396, 429, 458, 642
MboII GAAGA 3 cut(s) 193, 256, 274
MluCI AATT 3 cut(s) 328, 444, 603
MlyI GAGTC 1 cut(s) 233
MmeI TCCRAC 1 cut(s) 552
MseI TTAA 7 cut(s) 36, 111, 162, 327, 354, 534, 659
MspA1I CMGCKG 1 cut(s) 101
MspR9I CCNGG 2 cut(s) 456, 631
MvaI CCWGG 2 cut(s) 456, 631
MwoI GCNNNNNNNGC 1 cut(s) 209
NdeII GATC 4 cut(s) 396, 429, 458, 642
NheI GCTAGC 1 cut(s) 470
NlaIII CATG 1 cut(s) 718
NlaIV GGNNCC 1 cut(s) 507
NmuCI GTSAC 1 cut(s) 523
PcsI WCGNNNNNNNCGW 1 cut(s) 224
PfeI GAWTC 4 cut(s) 698, 719, 740, 761
PflMI CCANNNNNTGG 1 cut(s) 455
PfoI TCCNGGA 1 cut(s) 629
PleI GAGTC 1 cut(s) 232
PpsI GAGTC 1 cut(s) 232
Psp6I CCWGG 2 cut(s) 454, 629
PspGI CCWGG 2 cut(s) 454, 629
PspN4I GGNNCC 1 cut(s) 507
PstNI CAGNNNCTG 1 cut(s) 455
RsaI GTAC 1 cut(s) 495
RsaNI GTAC 1 cut(s) 494
SalI GTCGAC 1 cut(s) 10
SaqAI TTAA 7 cut(s) 36, 111, 162, 327, 354, 534, 659
Sau3AI GATC 4 cut(s) 396, 429, 458, 642
SchI GAGTC 1 cut(s) 233
ScrFI CCNGG 2 cut(s) 456, 631
SfcI CTRYAG 1 cut(s) 546
Sse9I AATT 3 cut(s) 328, 444, 603
SsiI CCGC 2 cut(s) 101, 474
SspMI CTAG 1 cut(s) 471
StyD4I CCNGG 2 cut(s) 454, 629
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 1 cut(s) 515
TaqI TCGA 5 cut(s) 11, 167, 300, 399, 461
TasI AATT 3 cut(s) 328, 444, 603
TfiI GAWTC 4 cut(s) 698, 719, 740, 761
Tru1I TTAA 7 cut(s) 36, 111, 162, 327, 354, 534, 659
Tru9I TTAA 7 cut(s) 36, 111, 162, 327, 354, 534, 659
TscAI CASTG 3 cut(s) 424, 430, 504
TseFI GTSAC 1 cut(s) 523
Tsp45I GTSAC 1 cut(s) 523
TspDTI ATGAA 1 cut(s) 86
TspGWI ACGGA 4 cut(s) 29, 71, 104, 233
TspRI CASTG 3 cut(s) 424, 430, 504
Van91I CCANNNNNTGG 1 cut(s) 455
XagI CCTNNNNNAGG 1 cut(s) 347
XapI RAATTY 1 cut(s) 444
XmiI GTMKAC 1 cut(s) 11
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.