RchiOBHm_Chr1g0357771

Desiccation protectant protein Lea14 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003820
Physical Location & Seq
Forward (+)
50065071 .. 50066151
1081 bp
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UTR
Exon/CDS
Intron
PRQ58299

Sequence Viewer

Length: 459 bp
ATGGCTCAGTTCTTTGACAAGGCCAAGAACTTCGTGACAGACAAACTGGCCAACGTAGAAAAGCCAGAGGCAGATCTCACAGATGTTGACTTCAGGAAGGCGAGCTTCGATTCCGTGGAGTACATCGGTAAGGTCCATGTCAAAAACCCCTATGATCACTCCATCCCCATCTGCGAGATCTCCTACACCCTCAAATGTGTTGGCAGGGAGATTATATCTGGGACAGTTCCGGACCCCGGATCAATTAAGGCAAGTGATGTTACCCTTCTGGAGGTACCAGTGAAGGTGCCACACAGTGTATTGATGACATTGGTGAAGGATGTTGGGGCAGATTGGGACATTGATTATGAGTTGATATTGGGTCTCACCATTGACCTTCCTGTGATTGGGAACTTCACCATACCTTTGTCGAAGAAGGGAGAGATCAAGCTTCCCAGCCTCACCAGCCTCTTTGCTTGA

Protein Analysis

152

Amino Acids

16.72

Weight (kDa)

4.99

Isoelectric Point (pI)

12.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 44 - 140 4.6e-22 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 274
AccB1I GGYRCC 2 cut(s) 274, 286
AccIII TCCGGA 1 cut(s) 229
AclWI GGATC 1 cut(s) 247
AcoI YGGCCR 1 cut(s) 48
AcuI CTGAAG 1 cut(s) 76
AdeI CACNNNGTG 1 cut(s) 296
AfaI GTAC 2 cut(s) 122, 276
AfiI CCNNNNNNNGG 3 cut(s) 236, 271, 386
AluBI AGCT 2 cut(s) 105, 430
AluI AGCT 2 cut(s) 105, 430
Alw26I GTCTC 1 cut(s) 368
AlwI GGATC 1 cut(s) 247
Aor13HI TCCGGA 1 cut(s) 229
AoxI GGCC 2 cut(s) 21, 48
Asp718I GGTACC 1 cut(s) 274
AspS9I GGNCC 2 cut(s) 133, 232
AsuC2I CCSGG 1 cut(s) 237
AsuHPI GGTGA 4 cut(s) 325, 358, 388, 433
AvaII GGWCC 2 cut(s) 133, 232
BalI TGGCCA 1 cut(s) 50
BanI GGYRCC 2 cut(s) 274, 286
BccI CCATC 2 cut(s) 170, 176
BclI TGATCA 1 cut(s) 154
BcnI CCSGG 1 cut(s) 237
BcoDI GTCTC 1 cut(s) 368
BglII AGATCT 2 cut(s) 73, 177
Bme1390I CCNGG 1 cut(s) 237
Bme18I GGWCC 2 cut(s) 133, 232
BmgT120I GGNCC 2 cut(s) 133, 232
BmiI GGNNCC 3 cut(s) 234, 276, 288
BmrFI CCNGG 1 cut(s) 237
BpmI CTGGAG 1 cut(s) 290
BpuMI CCSGG 1 cut(s) 237
BsaI GGTCTC 1 cut(s) 368
BsaJI CCNNGG 2 cut(s) 114, 235
BsaWI WCCGGW 1 cut(s) 229
Bsc4I CCNNNNNNNGG 3 cut(s) 236, 271, 386
Bse1I ACTGG 2 cut(s) 51, 278
BseAI TCCGGA 1 cut(s) 229
BseDI CCNNGG 2 cut(s) 114, 235
BseGI GGATG 2 cut(s) 162, 325
BseLI CCNNNNNNNGG 3 cut(s) 236, 271, 386
BseMII CTCAG 1 cut(s) 20
BseNI ACTGG 2 cut(s) 51, 278
BseYI CCCAGC 1 cut(s) 434
BshFI GGCC 2 cut(s) 23, 50
BshNI GGYRCC 2 cut(s) 274, 286
BsiSI CCGG 2 cut(s) 230, 237
BslFI GGGAC 2 cut(s) 235, 350
BslI CCNNNNNNNGG 3 cut(s) 236, 271, 386
BsmAI GTCTC 1 cut(s) 368
BsmFI GGGAC 2 cut(s) 235, 350
BsnI GGCC 2 cut(s) 23, 50
Bso31I GGTCTC 1 cut(s) 368
Bsp13I TCCGGA 1 cut(s) 229
Bsp143I GATC 5 cut(s) 73, 154, 177, 239, 423
BspANI GGCC 2 cut(s) 23, 50
BspCNI CTCAG 1 cut(s) 19
BspEI TCCGGA 1 cut(s) 229
BspLI GGNNCC 3 cut(s) 234, 276, 288
BspPI GGATC 1 cut(s) 247
BspT107I GGYRCC 2 cut(s) 274, 286
BspTNI GGTCTC 1 cut(s) 368
BsrI ACTGG 2 cut(s) 51, 278
BssECI CCNNGG 2 cut(s) 114, 235
BssMI GATC 5 cut(s) 73, 154, 177, 239, 423
Bst4CI ACNGT 2 cut(s) 226, 296
BstC8I GCNNGC 1 cut(s) 103
BstDEI CTNAG 1 cut(s) 6
BstDSI CCRYGG 1 cut(s) 114
BstENI CCTNNNNNAGG 1 cut(s) 269
BstF5I GGATG 2 cut(s) 162, 325
BstKTI GATC 5 cut(s) 76, 157, 180, 242, 426
BstMAI GTCTC 1 cut(s) 368
BstMBI GATC 5 cut(s) 73, 154, 177, 239, 423
BstMWI GCNNNNNNNGC 1 cut(s) 444
BstSCI CCNGG 1 cut(s) 235
BstX2I RGATCY 2 cut(s) 73, 177
BstYI RGATCY 2 cut(s) 73, 177
BsuRI GGCC 2 cut(s) 23, 50
BtgI CCRYGG 1 cut(s) 114
BtsCI GGATG 2 cut(s) 162, 325
BtsIMutI CAGTG 2 cut(s) 285, 301
Cac8I GCNNGC 1 cut(s) 103
Cfr13I GGNCC 2 cut(s) 133, 232
Csp6I GTAC 2 cut(s) 121, 275
CviAII CATG 1 cut(s) 137
CviJI RGCY 8 cut(s) 5, 23, 50, 64, 105, 430, 438, 447
CviKI_1 RGCY 8 cut(s) 5, 23, 50, 64, 105, 430, 438, 447
CviQI GTAC 2 cut(s) 121, 275
DdeI CTNAG 1 cut(s) 6
DpnI GATC 5 cut(s) 75, 156, 179, 241, 425
DpnII GATC 5 cut(s) 73, 154, 177, 239, 423
DraIII CACNNNGTG 1 cut(s) 296
EaeI YGGCCR 1 cut(s) 48
Eco31I GGTCTC 1 cut(s) 368
Eco47I GGWCC 2 cut(s) 133, 232
Eco57I CTGAAG 1 cut(s) 76
EcoNI CCTNNNNNAGG 1 cut(s) 269
FaeI CATG 1 cut(s) 140
FaiI YATR 5 cut(s) 138, 153, 215, 348, 401
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FaqI GGGAC 2 cut(s) 235, 350
FatI CATG 1 cut(s) 136
FbaI TGATCA 1 cut(s) 154
FokI GGATG 2 cut(s) 149, 332
GsaI CCCAGC 1 cut(s) 438
GsuI CTGGAG 1 cut(s) 290
HaeIII GGCC 2 cut(s) 23, 50
HapII CCGG 2 cut(s) 230, 237
Hin1II CATG 1 cut(s) 140
HincII GTYRAC 1 cut(s) 88
HindII GTYRAC 1 cut(s) 88
HindIII AAGCTT 1 cut(s) 428
HinfI GANTC 1 cut(s) 110
HpaII CCGG 2 cut(s) 230, 237
HphI GGTGA 4 cut(s) 325, 358, 388, 433
Hpy166II GTNNAC 1 cut(s) 88
Hpy188III TCNNGA 4 cut(s) 34, 94, 230, 269
Hpy8I GTNNAC 1 cut(s) 88
HpyAV CCTTC 6 cut(s) 91, 275, 277, 310, 386, 409
HpyCH4III ACNGT 2 cut(s) 226, 296
HpyCH4IV ACGT 1 cut(s) 54
HpyF10VI GCNNNNNNNGC 1 cut(s) 444
HpyF3I CTNAG 1 cut(s) 6
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 1 cut(s) 140
Kpn2I TCCGGA 1 cut(s) 229
KpnI GGTACC 1 cut(s) 278
Ksp22I TGATCA 1 cut(s) 154
Kzo9I GATC 5 cut(s) 73, 154, 177, 239, 423
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 2 cut(s) 34, 259
MalI GATC 5 cut(s) 75, 156, 179, 241, 425
MboI GATC 5 cut(s) 73, 154, 177, 239, 423
MboII GAAGA 1 cut(s) 424
MflI RGATCY 2 cut(s) 73, 177
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 1 cut(s) 243
MluNI TGGCCA 1 cut(s) 50
MnlI CCTC 5 cut(s) 61, 200, 265, 449, 458
Mox20I TGGCCA 1 cut(s) 50
MroI TCCGGA 1 cut(s) 229
MscI TGGCCA 1 cut(s) 50
MseI TTAA 1 cut(s) 246
Msp20I TGGCCA 1 cut(s) 50
MspI CCGG 2 cut(s) 230, 237
MspR9I CCNGG 1 cut(s) 237
MwoI GCNNNNNNNGC 1 cut(s) 444
NciI CCSGG 1 cut(s) 237
NdeII GATC 5 cut(s) 73, 154, 177, 239, 423
NlaIII CATG 1 cut(s) 140
NlaIV GGNNCC 3 cut(s) 234, 276, 288
NmuCI GTSAC 1 cut(s) 34
PfeI GAWTC 1 cut(s) 110
PspFI CCCAGC 1 cut(s) 434
PspN4I GGNNCC 3 cut(s) 234, 276, 288
PspPI GGNCC 2 cut(s) 133, 232
PsuI RGATCY 2 cut(s) 73, 177
RsaI GTAC 2 cut(s) 122, 276
RsaNI GTAC 2 cut(s) 121, 275
SaqAI TTAA 1 cut(s) 246
Sau3AI GATC 5 cut(s) 73, 154, 177, 239, 423
Sau96I GGNCC 2 cut(s) 133, 232
ScrFI CCNGG 1 cut(s) 237
SetI ASST 8 cut(s) 57, 107, 135, 276, 288, 378, 406, 432
SinI GGWCC 2 cut(s) 133, 232
Sse9I AATT 1 cut(s) 243
StyD4I CCNGG 1 cut(s) 235
TaaI ACNGT 2 cut(s) 226, 296
TaiI ACGT 1 cut(s) 57
TaqI TCGA 2 cut(s) 108, 410
TasI AATT 1 cut(s) 243
TatI WGTACW 1 cut(s) 120
TfiI GAWTC 1 cut(s) 110
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 2 cut(s) 285, 301
TseFI GTSAC 1 cut(s) 34
Tsp45I GTSAC 1 cut(s) 34
TspGWI ACGGA 1 cut(s) 103
TspRI CASTG 2 cut(s) 285, 301
VpaK11BI GGWCC 2 cut(s) 133, 232
XagI CCTNNNNNAGG 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.