MD07G1130800.v1.1

Desiccation protectant protein Lea14 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003820
Physical Location & Seq
Reverse (-)
18697800 .. 18699563
1764 bp
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UTR
Exon/CDS
Intron
MD07G1130800.v1.1.491

Sequence Viewer

Length: 453 bp
ATGGCCGAAGAAAGTTTCATGGATAAGGCCAAGAACTTCGTGGCAGAAAAGATAGGAAACATGGCGAAGCCAGAAGCAGAAGTCACAGATGTTGATTTCAAGAAGGTGAGCTTGAGCTCTGTGGAGTACCTTGCCAAGGTATCAGTGACCAATCCCTACAGCCACTCCATCCCCATCTGTGATATCAAATACACCCTCAAAAGCGTTAACAGGGAGATTGCATCCGGGACTATACCGGACCCTGGATCCATAAGGGCAAGTGATGTAACTGTGCTGGAGGTGCTATTGAAGGTTCCACACAGCATATTGTTGACATTGGTAAAGGATCTGGGTGCAGACTGGGACTTCGACTATGAATTGGACATAGGCCTCATCATTGACCTACCCGTCATCGGCAACTTTACCATACCACTCAACAAGAAGGGAGAGTTCAAGCTTCCCTCCCTCTTTTAA

Protein Analysis

151

Amino Acids

16.54

Weight (kDa)

4.9

Isoelectric Point (pI)

9.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 46 - 142 1.5e-21 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 386
AclWI GGATC 3 cut(s) 240, 253, 333
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 1 cut(s) 128
AfiI CCNNNNNNNGG 3 cut(s) 136, 242, 392
AgsI TTSAA 3 cut(s) 100, 289, 433
AjnI CCWGG 1 cut(s) 241
AluBI AGCT 3 cut(s) 111, 117, 436
AluI AGCT 3 cut(s) 111, 117, 436
Alw21I GWGCWC 1 cut(s) 119
AlwI GGATC 3 cut(s) 240, 253, 333
AoxI GGCC 3 cut(s) 3, 27, 367
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
AspS9I GGNCC 1 cut(s) 238
AsuC2I CCSGG 1 cut(s) 226
AsuHPI GGTGA 1 cut(s) 118
AvaII GGWCC 1 cut(s) 238
BamHI GGATCC 1 cut(s) 245
BanII GRGCYC 1 cut(s) 119
Bbv12I GWGCWC 1 cut(s) 119
BccI CCATC 2 cut(s) 176, 182
BciT130I CCWGG 1 cut(s) 243
BcnI CCSGG 1 cut(s) 226
BfmI CTRYAG 1 cut(s) 157
Bme1390I CCNGG 2 cut(s) 226, 243
Bme18I GGWCC 1 cut(s) 238
BmgT120I GGNCC 1 cut(s) 238
BmiI GGNNCC 3 cut(s) 240, 247, 294
BmrFI CCNGG 2 cut(s) 226, 243
BmrI ACTGGG 1 cut(s) 349
BmsI GCATC 1 cut(s) 230
BmuI ACTGGG 1 cut(s) 349
BpmI CTGGAG 1 cut(s) 296
BpuEI CTTGAG 1 cut(s) 133
BpuMI CCSGG 1 cut(s) 226
BsaJI CCNNGG 2 cut(s) 135, 241
BsaWI WCCGGW 1 cut(s) 235
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 242, 392
Bse1I ACTGG 1 cut(s) 344
BseBI CCWGG 1 cut(s) 243
BseDI CCNNGG 2 cut(s) 135, 241
BseGI GGATG 2 cut(s) 168, 221
BseLI CCNNNNNNNGG 3 cut(s) 136, 242, 392
BseNI ACTGG 1 cut(s) 344
BsgI GTGCAG 1 cut(s) 354
BshFI GGCC 3 cut(s) 5, 29, 369
BsiHKAI GWGCWC 1 cut(s) 119
BsiSI CCGG 2 cut(s) 225, 236
BslFI GGGAC 2 cut(s) 241, 356
BslI CCNNNNNNNGG 3 cut(s) 136, 242, 392
BsmFI GGGAC 2 cut(s) 241, 356
BsnI GGCC 3 cut(s) 5, 29, 369
Bsp1286I GDGCHC 1 cut(s) 119
Bsp143I GATC 2 cut(s) 245, 325
BspANI GGCC 3 cut(s) 5, 29, 369
BspLI GGNNCC 3 cut(s) 240, 247, 294
BspPI GGATC 3 cut(s) 240, 253, 333
BsrI ACTGG 1 cut(s) 344
BssECI CCNNGG 2 cut(s) 135, 241
BssMI GATC 2 cut(s) 245, 325
BssT1I CCWWGG 1 cut(s) 135
Bst2UI CCWGG 1 cut(s) 243
Bst4CI ACNGT 1 cut(s) 271
BstENI CCTNNNNNAGG 1 cut(s) 134
BstF5I GGATG 2 cut(s) 168, 221
BstKTI GATC 2 cut(s) 248, 328
BstMBI GATC 2 cut(s) 245, 325
BstMWI GCNNNNNNNGC 1 cut(s) 280
BstNI CCWGG 1 cut(s) 243
BstSCI CCNGG 2 cut(s) 224, 241
BstSFI CTRYAG 1 cut(s) 157
BstX2I RGATCY 2 cut(s) 245, 325
BstYI RGATCY 2 cut(s) 245, 325
BsuRI GGCC 3 cut(s) 5, 29, 369
BtsCI GGATG 2 cut(s) 168, 221
BtsIMutI CAGTG 1 cut(s) 150
Cfr13I GGNCC 1 cut(s) 238
Csp6I GTAC 1 cut(s) 127
CviAII CATG 2 cut(s) 19, 61
CviJI RGCY 8 cut(s) 5, 29, 70, 111, 117, 162, 369, 436
CviKI_1 RGCY 8 cut(s) 5, 29, 70, 111, 117, 162, 369, 436
CviQI GTAC 1 cut(s) 127
DpnI GATC 2 cut(s) 247, 327
DpnII GATC 2 cut(s) 245, 325
DrdI GACNNNNNNGTC 1 cut(s) 386
DseDI GACNNNNNNGTC 1 cut(s) 386
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 117
Eco130I CCWWGG 1 cut(s) 135
Eco147I AGGCCT 1 cut(s) 369
Eco24I GRGCYC 1 cut(s) 119
Eco32I GATATC 1 cut(s) 184
Eco47I GGWCC 1 cut(s) 238
Eco53kI GAGCTC 1 cut(s) 117
EcoICRI GAGCTC 1 cut(s) 117
EcoNI CCTNNNNNAGG 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 241
EcoRV GATATC 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 135
EcoT38I GRGCYC 1 cut(s) 119
ErhI CCWWGG 1 cut(s) 135
FaeI CATG 2 cut(s) 22, 64
FaiI YATR 8 cut(s) 20, 62, 233, 251, 305, 354, 365, 407
FalI AAGNNNNNCTT 2 cut(s) 95, 127
FaqI GGGAC 2 cut(s) 241, 356
FatI CATG 2 cut(s) 18, 60
FokI GGATG 2 cut(s) 155, 208
FriOI GRGCYC 1 cut(s) 119
GsuI CTGGAG 1 cut(s) 296
HaeIII GGCC 3 cut(s) 5, 29, 369
HapII CCGG 2 cut(s) 225, 236
Hin1II CATG 2 cut(s) 22, 64
HincII GTYRAC 2 cut(s) 208, 312
HindII GTYRAC 2 cut(s) 208, 312
HindIII AAGCTT 1 cut(s) 434
HpaI GTTAAC 1 cut(s) 208
HpaII CCGG 2 cut(s) 225, 236
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 2 cut(s) 208, 312
Hpy188III TCNNGA 1 cut(s) 100
Hpy8I GTNNAC 2 cut(s) 208, 312
HpyAV CCTTC 3 cut(s) 97, 283, 415
HpyCH4III ACNGT 1 cut(s) 271
HpyCH4V TGCA 2 cut(s) 221, 335
HpyF10VI GCNNNNNNNGC 1 cut(s) 280
Hsp92II CATG 2 cut(s) 22, 64
KspAI GTTAAC 1 cut(s) 208
Kzo9I GATC 2 cut(s) 245, 325
LpnPI CCDG 9 cut(s) 84, 196, 228, 238, 249, 255, 260, 314, 325
LweI GCATC 1 cut(s) 230
MaeIII GTNAC 3 cut(s) 82, 145, 265
MalI GATC 2 cut(s) 247, 327
MboI GATC 2 cut(s) 245, 325
MboII GAAGA 1 cut(s) 20
MflI RGATCY 2 cut(s) 245, 325
MhlI GDGCHC 1 cut(s) 119
MluCI AATT 1 cut(s) 356
MnlI CCTC 4 cut(s) 206, 271, 380, 451
MseI TTAA 2 cut(s) 207, 451
MspI CCGG 2 cut(s) 225, 236
MspR9I CCNGG 2 cut(s) 226, 243
MvaI CCWGG 1 cut(s) 243
MwoI GCNNNNNNNGC 1 cut(s) 280
NciI CCSGG 1 cut(s) 226
NdeII GATC 2 cut(s) 245, 325
NlaIII CATG 2 cut(s) 22, 64
NlaIV GGNNCC 3 cut(s) 240, 247, 294
NmuCI GTSAC 2 cut(s) 82, 145
PceI AGGCCT 1 cut(s) 369
PfoI TCCNGGA 1 cut(s) 224
Psp124BI GAGCTC 1 cut(s) 119
Psp6I CCWGG 1 cut(s) 241
PspGI CCWGG 1 cut(s) 241
PspN4I GGNNCC 3 cut(s) 240, 247, 294
PspPI GGNCC 1 cut(s) 238
PsuI RGATCY 2 cut(s) 245, 325
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
SacI GAGCTC 1 cut(s) 119
SaqAI TTAA 2 cut(s) 207, 451
Sau3AI GATC 2 cut(s) 245, 325
Sau96I GGNCC 1 cut(s) 238
ScrFI CCNGG 2 cut(s) 226, 243
SduI GDGCHC 1 cut(s) 119
SetI ASST 9 cut(s) 108, 113, 119, 132, 141, 282, 294, 384, 438
SfaNI GCATC 1 cut(s) 230
SfcI CTRYAG 1 cut(s) 157
SinI GGWCC 1 cut(s) 238
SmlI CTYRAG 1 cut(s) 112
SmoI CTYRAG 1 cut(s) 112
Sse9I AATT 1 cut(s) 356
SseBI AGGCCT 1 cut(s) 369
SstI GAGCTC 1 cut(s) 119
StuI AGGCCT 1 cut(s) 369
StyD4I CCNGG 2 cut(s) 224, 241
StyI CCWWGG 1 cut(s) 135
TaaI ACNGT 1 cut(s) 271
TaqI TCGA 1 cut(s) 348
TasI AATT 1 cut(s) 356
Tru1I TTAA 2 cut(s) 207, 451
Tru9I TTAA 2 cut(s) 207, 451
TscAI CASTG 1 cut(s) 150
TseFI GTSAC 2 cut(s) 82, 145
Tsp45I GTSAC 2 cut(s) 82, 145
TspDTI ATGAA 2 cut(s) 7, 369
TspRI CASTG 1 cut(s) 150
VpaK11BI GGWCC 1 cut(s) 238
XagI CCTNNNNNAGG 1 cut(s) 134
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.