FvH4_7g21560

helix loop helix domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002277
Physical Location & Seq
Reverse (-)
17220360 .. 17223499
3140 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g21560.t4

Sequence Viewer

Length: 879 bp
ATGAGAAACGCTCTTCTTCCTTCCCATCTTTACAACTTCGGACACACTGATTTTCAGATGACAAATTGGGATTCTCAGATGAACATGAACAGAAAGATTGGCTTTTGCATGGGGTCCAAAGCAAATGAAAGTGACAACCATAACAGAATTTCTGGAGACTTTTCTGATGTTCCTCTTGATCAGTCAGCACTCTTTACCTATCAAACAGCAGTAACAGCAGGTTATTCCACTGGAGAATTCAGATATGATCATGGCATTTTTCAGCAGCCACTATTGTTTGACAAAAGTATTGATTCAGCTACCCATTTTGAAGAAGATCTTCAGGTCATACAAAATGGTAGGAAGCGTCAAATAATTGAGGTCAATGACATCTTTCCCAAAAATGACCTCGGTGGTTCAATTGGTGAGAGAACAACTTCAAATGAATGGAACAAAAACAAGAGAAGCAAGGTGACAAATTCTCAACAACAATGGCATAATCAATTCCAAGAAATATCTATGGAAACACAAGACAATAGGAAGTTGCAAGTTCCTGTGAGGAGAAGCCAAAAGCTTAGTGACAAAATCACTGCTCTTCAAAAATTGGTTTCCCCCTATGGCAAGACTGACACTGCCTCAGTTCTTCAAGAGGCTTCGGTTTACATCGTGCTCCTTCAACAACAAATTCAGAATCTGCATCGGATCTTTAGTAGTTCATACATCAATGCAGTAGGACTTCATTCACAAGAATCTGGGAGTAGTAGCCAAGTGCTTGACCTCAGAAGCAAAGGCCTTTGTTTGGTTCCCATTTCGATGACTCAGAAGGTGACTATGGAAGAGCGTTTTGATGAGGATCATCTTTCTACATCAAGAAATTTTGTCATAGCTAACCATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
No domains found.

Protein Analysis

293

Amino Acids

33.36

Weight (kDa)

6.55

Isoelectric Point (pI)

49.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 209
AclWI GGATC 2 cut(s) 689, 840
AcsI RAATTY 5 cut(s) 147, 236, 457, 663, 853
AcuI CTGAAG 1 cut(s) 305
AfiI CCNNNNNNNGG 1 cut(s) 778
AgsI TTSAA 6 cut(s) 311, 399, 420, 578, 626, 656
AluBI AGCT 3 cut(s) 299, 553, 866
AluI AGCT 3 cut(s) 299, 553, 866
Alw21I GWGCWC 1 cut(s) 651
Alw26I GTCTC 1 cut(s) 150
AlwI GGATC 2 cut(s) 689, 840
AlwNI CAGNNNCTG 1 cut(s) 673
AoxI GGCC 1 cut(s) 769
ApeKI GCWGC 1 cut(s) 265
ApoI RAATTY 5 cut(s) 147, 236, 457, 663, 853
Asp700I GAANNNNTTC 2 cut(s) 318, 415
AspS9I GGNCC 1 cut(s) 114
AsuHPI GGTGA 3 cut(s) 416, 463, 817
AvaII GGWCC 1 cut(s) 114
Bbv12I GWGCWC 1 cut(s) 651
BbvI GCAGC 1 cut(s) 277
BccI CCATC 1 cut(s) 33
BclI TGATCA 2 cut(s) 178, 247
BcoDI GTCTC 1 cut(s) 150
BfaI CTAG 1 cut(s) 877
BfuAI ACCTGC 1 cut(s) 209
BglII AGATCT 1 cut(s) 316
BisI GCNGC 1 cut(s) 266
BlsI GCNGC 1 cut(s) 267
Bme18I GGWCC 1 cut(s) 114
BmgT120I GGNCC 1 cut(s) 114
BmiI GGNNCC 2 cut(s) 115, 783
BmsI GCATC 1 cut(s) 685
BplI GAGNNNNNCTC 1 cut(s) 27
BpmI CTGGAG 2 cut(s) 174, 252
BsaBI GATNNNNATC 1 cut(s) 831
BsaJI CCNNGG 1 cut(s) 388
Bsc4I CCNNNNNNNGG 1 cut(s) 778
Bse1I ACTGG 1 cut(s) 235
Bse8I GATNNNNATC 1 cut(s) 831
BseDI CCNNGG 1 cut(s) 388
BseJI GATNNNNATC 1 cut(s) 831
BseLI CCNNNNNNNGG 1 cut(s) 778
BseMII CTCAG 4 cut(s) 89, 630, 772, 812
BseNI ACTGG 1 cut(s) 235
BseRI GAGGAG 1 cut(s) 553
BseXI GCAGC 1 cut(s) 277
BshFI GGCC 1 cut(s) 771
BsiHKAI GWGCWC 1 cut(s) 651
BslI CCNNNNNNNGG 1 cut(s) 778
BsmAI GTCTC 1 cut(s) 150
BsnI GGCC 1 cut(s) 771
Bsp1286I GDGCHC 1 cut(s) 651
Bsp143I GATC 5 cut(s) 178, 247, 316, 681, 832
BspANI GGCC 1 cut(s) 771
BspCNI CTCAG 4 cut(s) 88, 629, 771, 811
BspLI GGNNCC 2 cut(s) 115, 783
BspMI ACCTGC 1 cut(s) 209
BspPI GGATC 2 cut(s) 689, 840
BspQI GCTCTTC 3 cut(s) 18, 579, 810
BsrI ACTGG 1 cut(s) 235
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 5 cut(s) 178, 247, 316, 681, 832
Bst6I CTCTTC 3 cut(s) 18, 579, 810
BstDEI CTNAG 5 cut(s) 75, 554, 616, 758, 798
BstKTI GATC 5 cut(s) 181, 250, 319, 684, 835
BstMAI GTCTC 1 cut(s) 150
BstMBI GATC 5 cut(s) 178, 247, 316, 681, 832
BstMWI GCNNNNNNNGC 1 cut(s) 215
BstV1I GCAGC 1 cut(s) 277
BstX2I RGATCY 2 cut(s) 316, 681
BstYI RGATCY 2 cut(s) 316, 681
BsuRI GGCC 1 cut(s) 771
BtsI GCAGTG 2 cut(s) 567, 609
BtsIMutI CAGTG 4 cut(s) 45, 228, 567, 609
BveI ACCTGC 1 cut(s) 209
CaiI CAGNNNCTG 1 cut(s) 673
Cfr13I GGNCC 1 cut(s) 114
CseI GACGC 1 cut(s) 335
CviAII CATG 3 cut(s) 85, 109, 251
CviJI RGCY 9 cut(s) 102, 268, 299, 546, 553, 632, 744, 771, 866
CviKI_1 RGCY 9 cut(s) 102, 268, 299, 546, 553, 632, 744, 771, 866
DdeI CTNAG 5 cut(s) 75, 554, 616, 758, 798
DpnI GATC 5 cut(s) 180, 249, 318, 683, 834
DpnII GATC 5 cut(s) 178, 247, 316, 681, 832
Eam1104I CTCTTC 3 cut(s) 18, 579, 810
EarI CTCTTC 3 cut(s) 18, 579, 810
Eco147I AGGCCT 1 cut(s) 771
Eco47I GGWCC 1 cut(s) 114
Eco57I CTGAAG 1 cut(s) 305
EcoRI GAATTC 1 cut(s) 236
FaeI CATG 3 cut(s) 88, 112, 254
FalI AAGNNNNNCTT 4 cut(s) 86, 118, 303, 335
FatI CATG 3 cut(s) 84, 108, 250
FbaI TGATCA 2 cut(s) 178, 247
Fnu4HI GCNGC 1 cut(s) 266
Fsp4HI GCNGC 1 cut(s) 266
FspBI CTAG 1 cut(s) 877
GluI GCNGC 1 cut(s) 266
GsuI CTGGAG 2 cut(s) 174, 252
HaeIII GGCC 1 cut(s) 771
HgaI GACGC 1 cut(s) 335
Hin1II CATG 3 cut(s) 88, 112, 254
HindIII AAGCTT 1 cut(s) 551
HinfI GANTC 5 cut(s) 71, 293, 670, 728, 796
HphI GGTGA 3 cut(s) 416, 463, 817
Hpy166II GTNNAC 1 cut(s) 640
Hpy188I TCNGA 9 cut(s) 41, 57, 78, 166, 242, 669, 681, 761, 801
Hpy188III TCNNGA 4 cut(s) 153, 176, 626, 849
Hpy8I GTNNAC 1 cut(s) 640
HpyAV CCTTC 3 cut(s) 30, 662, 796
HpyCH4V TGCA 4 cut(s) 108, 526, 676, 707
HpyF10VI GCNNNNNNNGC 1 cut(s) 215
HpyF3I CTNAG 5 cut(s) 75, 554, 616, 758, 798
Hsp92II CATG 3 cut(s) 88, 112, 254
Ksp22I TGATCA 2 cut(s) 178, 247
Kzo9I GATC 5 cut(s) 178, 247, 316, 681, 832
LguI GCTCTTC 3 cut(s) 18, 579, 810
LmnI GCTCC 1 cut(s) 654
LpnPI CCDG 6 cut(s) 138, 204, 216, 308, 546, 717
Lsp1109I GCAGC 1 cut(s) 277
LweI GCATC 1 cut(s) 685
MaeI CTAG 1 cut(s) 877
MaeIII GTNAC 5 cut(s) 131, 211, 451, 557, 805
MalI GATC 5 cut(s) 180, 249, 318, 683, 834
MboI GATC 5 cut(s) 178, 247, 316, 681, 832
MboII GAAGA 8 cut(s) 5, 8, 311, 323, 326, 566, 614, 827
MfeI CAATTG 1 cut(s) 399
MflI RGATCY 2 cut(s) 316, 681
MhlI GDGCHC 1 cut(s) 651
MlyI GAGTC 1 cut(s) 790
MnlI CCTC 8 cut(s) 183, 352, 398, 531, 622, 625, 767, 823
MroXI GAANNNNTTC 2 cut(s) 318, 415
MslI CAYNNNNRTG 1 cut(s) 791
MunI CAATTG 1 cut(s) 399
MwoI GCNNNNNNNGC 1 cut(s) 215
NdeII GATC 5 cut(s) 178, 247, 316, 681, 832
NlaIII CATG 3 cut(s) 88, 112, 254
NlaIV GGNNCC 2 cut(s) 115, 783
NmuCI GTSAC 4 cut(s) 131, 451, 557, 805
PceI AGGCCT 1 cut(s) 771
PciSI GCTCTTC 3 cut(s) 18, 579, 810
PdmI GAANNNNTTC 2 cut(s) 318, 415
PfeI GAWTC 4 cut(s) 71, 293, 670, 728
PkrI GCNGC 1 cut(s) 267
PleI GAGTC 1 cut(s) 790
PpsI GAGTC 1 cut(s) 790
PspN4I GGNNCC 2 cut(s) 115, 783
PspPI GGNCC 1 cut(s) 114
PstNI CAGNNNCTG 1 cut(s) 673
PsuI RGATCY 2 cut(s) 316, 681
RseI CAYNNNNRTG 1 cut(s) 791
SapI GCTCTTC 3 cut(s) 18, 579, 810
SatI GCNGC 1 cut(s) 266
Sau3AI GATC 5 cut(s) 178, 247, 316, 681, 832
Sau96I GGNCC 1 cut(s) 114
SchI GAGTC 1 cut(s) 790
SduI GDGCHC 1 cut(s) 651
SfaNI GCATC 1 cut(s) 685
SinI GGWCC 1 cut(s) 114
SmiMI CAYNNNNRTG 1 cut(s) 791
SseBI AGGCCT 1 cut(s) 771
SspMI CTAG 1 cut(s) 877
StuI AGGCCT 1 cut(s) 771
TaqI TCGA 1 cut(s) 791
TfiI GAWTC 4 cut(s) 71, 293, 670, 728
TscAI CASTG 4 cut(s) 52, 235, 574, 616
TseFI GTSAC 4 cut(s) 131, 451, 557, 805
TseI GCWGC 1 cut(s) 265
Tsp45I GTSAC 4 cut(s) 131, 451, 557, 805
TspDTI ATGAA 6 cut(s) 95, 101, 141, 438, 684, 707
TspRI CASTG 4 cut(s) 52, 235, 574, 616
VpaK11BI GGWCC 1 cut(s) 114
XapI RAATTY 5 cut(s) 147, 236, 457, 663, 853
XmnI GAANNNNTTC 2 cut(s) 318, 415
XspI CTAG 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.