Rh1AG334300

helix loop helix domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0002277
Physical Location & Seq
Reverse (-)
56862622 .. 56864941
2320 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG334300.1

Sequence Viewer

Length: 873 bp
ATGAGTAACGCTCTTCTTAATTCCCATCTTTACAACTTTGGAAATACTGATTTTCAGATGATAAATTGGGATACTCAAATGAACATGAATAGAAAGATTGGCTTTTGCATGGGGTCCAAAGCAATAGAAAGTGACAACGAAACAGGAGACATTTCTGATGTTCCTCTTGATCAGTCATCACTCTTTACCTATCAGACAGCAGTAACAGCAGGTTATTACAATGGGGAATTCAGATATGATCATGGCATTTTTCAGCAGCCAATGTTGTTTGACAAGAATATTAATTCAGCTACCCAATTTCAAGAGGATTTGCAGGTCATACAGAATGCAAGGAAGCGCCCAATAATTGAGGTCAATGATATCTTGCCCAAAAATGACCTTGGTGGTTCAATTGGTGAGAGAACAACTTCAAATGAATGGAACCAAAACAAGAGAAACAAGGGGACAAATTCTCAACAACAATGGCATAATCAATTCCAAGAAGCATCTATGGAAAAACAAGACAATAGGAAGTTGCAAGTTCCTGTGAGGCGAAGCCAAAAGCTTAGTGACAAAATCACTGCTCTTCAAAAATTGGTTTCCCCCTATGGCAAGACTGACACTGCATCAGTTCTTCAAGAGGCTTCGATTTACATCGTTCTTCTTCAACAACAAATTCAGCAGAATCTGCATCGGATCTTTAGTAGTTCATACAAGAATGCTGCAGGACTTCACACACAAGAATCTGGGAGTAGTAGCCAAGTGCTTGACCTCAGAAGCAATGGCCTTTGTTTGGTTCCGATTTCCTTGACTCAGAAAGTGACTATGGAAGAGGGTTTTGATGAGGATCATCTTTCCACATCAAGAAAATTTGTCATAGCTGGCCATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
No domains found.

Protein Analysis

290

Amino Acids

32.93

Weight (kDa)

6.17

Isoelectric Point (pI)

49.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 200, 304
AclWI GGATC 2 cut(s) 683, 834
AcoI YGGCCR 1 cut(s) 862
AcsI RAATTY 4 cut(s) 227, 448, 654, 848
AfiI CCNNNNNNNGG 1 cut(s) 772
AgsI TTSAA 6 cut(s) 302, 390, 411, 569, 617, 647
AluBI AGCT 3 cut(s) 290, 544, 860
AluI AGCT 3 cut(s) 290, 544, 860
Alw26I GTCTC 1 cut(s) 141
AlwI GGATC 2 cut(s) 683, 834
AlwNI CAGNNNCTG 1 cut(s) 667
AoxI GGCC 2 cut(s) 763, 862
ApeKI GCWGC 2 cut(s) 256, 701
ApoI RAATTY 4 cut(s) 227, 448, 654, 848
AseI ATTAAT 1 cut(s) 282
Asp700I GAANNNNTTC 1 cut(s) 406
AspLEI GCGC 1 cut(s) 339
AspS9I GGNCC 1 cut(s) 114
AsuHPI GGTGA 1 cut(s) 407
AvaII GGWCC 1 cut(s) 114
BalI TGGCCA 1 cut(s) 864
BbvI GCAGC 2 cut(s) 268, 688
BccI CCATC 1 cut(s) 33
BciVI GTATCC 1 cut(s) 64
BclI TGATCA 2 cut(s) 169, 238
BcoDI GTCTC 1 cut(s) 141
BfaI CTAG 1 cut(s) 871
BfmI CTRYAG 1 cut(s) 702
BfoI RGCGCY 1 cut(s) 340
BfuAI ACCTGC 2 cut(s) 200, 304
BfuI GTATCC 1 cut(s) 64
BisI GCNGC 2 cut(s) 257, 702
BlsI GCNGC 2 cut(s) 258, 703
Bme18I GGWCC 1 cut(s) 114
BmgT120I GGNCC 1 cut(s) 114
BmiI GGNNCC 3 cut(s) 115, 422, 777
BmsI GCATC 3 cut(s) 494, 614, 679
BplI GAGNNNNNCTC 1 cut(s) 27
BsaBI GATNNNNATC 2 cut(s) 632, 825
BsaJI CCNNGG 1 cut(s) 379
Bsc4I CCNNNNNNNGG 1 cut(s) 772
Bse3DI GCAATG 1 cut(s) 766
Bse8I GATNNNNATC 2 cut(s) 632, 825
BseDI CCNNGG 1 cut(s) 379
BseJI GATNNNNATC 2 cut(s) 632, 825
BseLI CCNNNNNNNGG 1 cut(s) 772
BseMI GCAATG 1 cut(s) 766
BseMII CTCAG 2 cut(s) 766, 806
BseXI GCAGC 2 cut(s) 268, 688
BshFI GGCC 2 cut(s) 765, 864
BslFI GGGAC 1 cut(s) 457
BslI CCNNNNNNNGG 1 cut(s) 772
BsmAI GTCTC 1 cut(s) 141
BsmFI GGGAC 1 cut(s) 457
BsmI GAATGC 2 cut(s) 331, 703
BsnI GGCC 2 cut(s) 765, 864
Bsp143I GATC 4 cut(s) 169, 238, 675, 826
BspANI GGCC 2 cut(s) 765, 864
BspCNI CTCAG 2 cut(s) 765, 805
BspLI GGNNCC 3 cut(s) 115, 422, 777
BspMAI CTGCAG 1 cut(s) 706
BspMI ACCTGC 2 cut(s) 200, 304
BspPI GGATC 2 cut(s) 683, 834
BspQI GCTCTTC 2 cut(s) 18, 570
BsrDI GCAATG 1 cut(s) 766
BssECI CCNNGG 1 cut(s) 379
BssMI GATC 4 cut(s) 169, 238, 675, 826
BssT1I CCWWGG 1 cut(s) 379
Bst6I CTCTTC 3 cut(s) 18, 570, 804
BstAPI GCANNNNNTGC 1 cut(s) 667
BstC8I GCNNGC 1 cut(s) 862
BstDEI CTNAG 3 cut(s) 545, 752, 792
BstH2I RGCGCY 1 cut(s) 340
BstHHI GCGC 1 cut(s) 339
BstKTI GATC 4 cut(s) 172, 241, 678, 829
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 4 cut(s) 169, 238, 675, 826
BstMWI GCNNNNNNNGC 2 cut(s) 206, 667
BstSFI CTRYAG 1 cut(s) 702
BstV1I GCAGC 2 cut(s) 268, 688
BstX2I RGATCY 1 cut(s) 675
BstYI RGATCY 1 cut(s) 675
BsuI GTATCC 1 cut(s) 64
BsuRI GGCC 2 cut(s) 765, 864
BtsI GCAGTG 2 cut(s) 558, 600
BtsIMutI CAGTG 2 cut(s) 558, 600
BveI ACCTGC 2 cut(s) 200, 304
Cac8I GCNNGC 1 cut(s) 862
CaiI CAGNNNCTG 1 cut(s) 667
CfoI GCGC 1 cut(s) 339
Cfr13I GGNCC 1 cut(s) 114
CviAII CATG 3 cut(s) 85, 109, 242
DdeI CTNAG 3 cut(s) 545, 752, 792
DpnI GATC 4 cut(s) 171, 240, 677, 828
DpnII GATC 4 cut(s) 169, 238, 675, 826
EaeI YGGCCR 1 cut(s) 862
Eam1104I CTCTTC 3 cut(s) 18, 570, 804
EarI CTCTTC 3 cut(s) 18, 570, 804
Eco130I CCWWGG 1 cut(s) 379
Eco32I GATATC 1 cut(s) 361
Eco47I GGWCC 1 cut(s) 114
EcoRI GAATTC 1 cut(s) 227
EcoRV GATATC 1 cut(s) 361
EcoT14I CCWWGG 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 379
FaeI CATG 3 cut(s) 88, 112, 245
FalI AAGNNNNNCTT 2 cut(s) 86, 118
FaqI GGGAC 1 cut(s) 457
FatI CATG 3 cut(s) 84, 108, 241
FbaI TGATCA 2 cut(s) 169, 238
Fnu4HI GCNGC 2 cut(s) 257, 702
Fsp4HI GCNGC 2 cut(s) 257, 702
FspBI CTAG 1 cut(s) 871
GlaI GCGC 1 cut(s) 338
GluI GCNGC 2 cut(s) 257, 702
HaeII RGCGCY 1 cut(s) 340
HaeIII GGCC 2 cut(s) 765, 864
HhaI GCGC 1 cut(s) 339
Hin1II CATG 3 cut(s) 88, 112, 245
Hin6I GCGC 1 cut(s) 337
HinP1I GCGC 1 cut(s) 337
HindIII AAGCTT 1 cut(s) 542
HinfI GANTC 3 cut(s) 664, 722, 790
HphI GGTGA 1 cut(s) 407
Hpy188I TCNGA 8 cut(s) 57, 157, 195, 233, 675, 755, 780, 795
Hpy188III TCNNGA 4 cut(s) 167, 302, 617, 843
HpyCH4V TGCA 7 cut(s) 108, 313, 329, 517, 605, 670, 704
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 667
HpyF3I CTNAG 3 cut(s) 545, 752, 792
Hsp92II CATG 3 cut(s) 88, 112, 245
HspAI GCGC 1 cut(s) 337
Ksp22I TGATCA 2 cut(s) 169, 238
Kzo9I GATC 4 cut(s) 169, 238, 675, 826
LguI GCTCTTC 2 cut(s) 18, 570
LpnPI CCDG 7 cut(s) 129, 195, 299, 537, 690, 711, 846
Lsp1109I GCAGC 2 cut(s) 268, 688
LweI GCATC 3 cut(s) 494, 614, 679
MaeI CTAG 1 cut(s) 871
MaeIII GTNAC 5 cut(s) 5, 131, 202, 548, 799
MalI GATC 4 cut(s) 171, 240, 677, 828
MboI GATC 4 cut(s) 169, 238, 675, 826
MboII GAAGA 6 cut(s) 5, 557, 605, 632, 635, 821
MfeI CAATTG 1 cut(s) 390
MflI RGATCY 1 cut(s) 675
MlsI TGGCCA 1 cut(s) 864
MluNI TGGCCA 1 cut(s) 864
MlyI GAGTC 1 cut(s) 784
MnlI CCTC 8 cut(s) 174, 298, 343, 522, 613, 761, 805, 817
Mox20I TGGCCA 1 cut(s) 864
MroXI GAANNNNTTC 1 cut(s) 406
MscI TGGCCA 1 cut(s) 864
MseI TTAA 2 cut(s) 18, 282
Msp20I TGGCCA 1 cut(s) 864
MunI CAATTG 1 cut(s) 390
Mva1269I GAATGC 2 cut(s) 331, 703
MwoI GCNNNNNNNGC 2 cut(s) 206, 667
NdeII GATC 4 cut(s) 169, 238, 675, 826
NlaIII CATG 3 cut(s) 88, 112, 245
NlaIV GGNNCC 3 cut(s) 115, 422, 777
NmuCI GTSAC 3 cut(s) 131, 548, 799
PciSI GCTCTTC 2 cut(s) 18, 570
PctI GAATGC 2 cut(s) 331, 703
PdmI GAANNNNTTC 1 cut(s) 406
PfeI GAWTC 2 cut(s) 664, 722
PkrI GCNGC 2 cut(s) 258, 703
PleI GAGTC 1 cut(s) 784
PpsI GAGTC 1 cut(s) 784
PshBI ATTAAT 1 cut(s) 282
PspN4I GGNNCC 3 cut(s) 115, 422, 777
PspPI GGNCC 1 cut(s) 114
PstI CTGCAG 1 cut(s) 706
PstNI CAGNNNCTG 1 cut(s) 667
PsuI RGATCY 1 cut(s) 675
SapI GCTCTTC 2 cut(s) 18, 570
SaqAI TTAA 2 cut(s) 18, 282
SatI GCNGC 2 cut(s) 257, 702
Sau3AI GATC 4 cut(s) 169, 238, 675, 826
Sau96I GGNCC 1 cut(s) 114
SchI GAGTC 1 cut(s) 784
SetI ASST 9 cut(s) 191, 214, 292, 318, 354, 381, 546, 753, 862
SfaNI GCATC 3 cut(s) 494, 614, 679
SfcI CTRYAG 1 cut(s) 702
SinI GGWCC 1 cut(s) 114
SspI AATATT 1 cut(s) 280
SspMI CTAG 1 cut(s) 871
StyI CCWWGG 1 cut(s) 379
TaqI TCGA 1 cut(s) 626
TfiI GAWTC 2 cut(s) 664, 722
Tru1I TTAA 2 cut(s) 18, 282
Tru9I TTAA 2 cut(s) 18, 282
TscAI CASTG 2 cut(s) 565, 607
TseFI GTSAC 3 cut(s) 131, 548, 799
TseI GCWGC 2 cut(s) 256, 701
Tsp45I GTSAC 3 cut(s) 131, 548, 799
TspDTI ATGAA 4 cut(s) 95, 101, 429, 678
TspRI CASTG 2 cut(s) 565, 607
VpaK11BI GGWCC 1 cut(s) 114
VspI ATTAAT 1 cut(s) 282
XapI RAATTY 4 cut(s) 227, 448, 654, 848
XmnI GAANNNNTTC 1 cut(s) 406
XspI CTAG 1 cut(s) 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.