MD01G1122500.v1.1

helix loop helix domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002277
Physical Location & Seq
Reverse (-)
23568825 .. 23572023
3199 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1122500.v1.1.491

Sequence Viewer

Length: 867 bp
ATGAGAAACACTGATCTCCTTCCATCTCTACCCTCCCATCTTTACAGCTGCGAAAACAATATTGATGTTCAGATGACAAATTGGGATGCACAAATGGACATGAACAGTAAAATTGGGTTTTGCATGGGATCCAACGCATTCGAAAGTGACAACCATGATAACATGAGAGCTGCAGACTATTCTCTTCCTCTGCAGCAGTCATCATCCCTCATTACCTATCAACCTCCAGTTAATTATCCATCTGGTTCGTTGAGATATGATGACATTTCAGAGCAGTCAATGTTCCTGGACAAAAAGATTTCAGCCCCCCAATTTCAAGCAGATTGCCAGGCCATACAAAATGCAAGGAAACGTCCAATTGAGGTTGATGACTTTGGTGGTCTTGTTGGTGACAGAACATCTCTCAACGAATTATACAAAAATAAGAGAAGCAAGATGACAAATAATAATTCTCAACAACAATATTGGCATCAACAGCTCCAAGAAAACTCAATGGAGCAACAAAACAATAAGAAGTTGCATCATGTGCCTGTGAGAAGAAGCCAAAAGCTTAGTGACAAAATCACAGCTCTCCAAAAATTGGTTTCCCCCTATGGCAAGACTGACACTGCCTCAGTTCTTCAAGAGGCTTCCACATATATCATGCTCCTCCAAGAACAAATTCAGAATCTGCTAAGGATGCTTGGTAATTCATACAAGAATGCTGCGGCTCTTCACTCACAGGAATCTGGGAGTGGGCAGGCGCTTGACCTCAGAAGCAAAGGCCTTTGCTTGGTTCCTGTGTCAATTACTCAGAAAGTGACTATGGAAGAGGGTTCTGATCATCTTGCTCCGTCAAGAAAAATTGTCATAGCTAATAACTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
No domains found.

Protein Analysis

289

Amino Acids

32.51

Weight (kDa)

6.65

Isoelectric Point (pI)

58.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 580
AciI CCGC 1 cut(s) 707
AclWI GGATC 2 cut(s) 123, 136
AcsI RAATTY 1 cut(s) 660
AfiI CCNNNNNNNGG 2 cut(s) 580, 772
AgsI TTSAA 2 cut(s) 317, 623
AjnI CCWGG 2 cut(s) 285, 327
AjuI GAANNNNNNNTTGG 2 cut(s) 645, 677
AluBI AGCT 6 cut(s) 48, 170, 478, 550, 569, 854
AluI AGCT 6 cut(s) 48, 170, 478, 550, 569, 854
AlwI GGATC 2 cut(s) 123, 136
AlwNI CAGNNNCTG 1 cut(s) 670
AoxI GGCC 2 cut(s) 330, 763
ApeKI GCWGC 4 cut(s) 48, 170, 193, 704
ApoI RAATTY 1 cut(s) 660
Asp700I GAANNNNTTC 1 cut(s) 660
AspLEI GCGC 1 cut(s) 745
AsuHPI GGTGA 1 cut(s) 401
AsuII TTCGAA 1 cut(s) 141
BamHI GGATCC 1 cut(s) 128
BbvI GCAGC 4 cut(s) 35, 157, 205, 691
BccI CCATC 3 cut(s) 31, 45, 247
BciT130I CCWGG 2 cut(s) 287, 329
BclI TGATCA 1 cut(s) 820
BfaI CTAG 1 cut(s) 865
BfmI CTRYAG 2 cut(s) 171, 191
BfoI RGCGCY 1 cut(s) 746
BisI GCNGC 5 cut(s) 49, 171, 194, 705, 708
BlsI GCNGC 5 cut(s) 50, 172, 195, 706, 709
Bme1390I CCNGG 2 cut(s) 287, 329
BmiI GGNNCC 2 cut(s) 130, 777
BmrFI CCNGG 2 cut(s) 287, 329
BmsI GCATC 4 cut(s) 76, 478, 529, 669
BpmI CTGGAG 1 cut(s) 210
Bpu10I CCTNAGC 1 cut(s) 674
Bpu14I TTCGAA 1 cut(s) 141
Bsc4I CCNNNNNNNGG 2 cut(s) 580, 772
Bse1I ACTGG 1 cut(s) 227
BseBI CCWGG 2 cut(s) 287, 329
BseGI GGATG 3 cut(s) 91, 203, 684
BseLI CCNNNNNNNGG 2 cut(s) 580, 772
BseMII CTCAG 3 cut(s) 627, 766, 806
BseNI ACTGG 1 cut(s) 227
BseRI GAGGAG 1 cut(s) 638
BseXI GCAGC 4 cut(s) 35, 157, 205, 691
BshFI GGCC 2 cut(s) 332, 765
BslI CCNNNNNNNGG 2 cut(s) 580, 772
BsmI GAATGC 2 cut(s) 137, 706
BsnI GGCC 2 cut(s) 332, 765
Bsp119I TTCGAA 1 cut(s) 141
Bsp143I GATC 3 cut(s) 13, 128, 820
BspACI CCGC 1 cut(s) 707
BspANI GGCC 2 cut(s) 332, 765
BspCNI CTCAG 3 cut(s) 626, 765, 805
BspLI GGNNCC 2 cut(s) 130, 777
BspMAI CTGCAG 2 cut(s) 175, 195
BspPI GGATC 2 cut(s) 123, 136
BspQI GCTCTTC 1 cut(s) 717
BspT104I TTCGAA 1 cut(s) 141
BsrI ACTGG 1 cut(s) 227
BssMI GATC 3 cut(s) 13, 128, 820
Bst2UI CCWGG 2 cut(s) 287, 329
Bst4CI ACNGT 1 cut(s) 107
Bst6I CTCTTC 3 cut(s) 189, 717, 804
BstAPI GCANNNNNTGC 1 cut(s) 526
BstBI TTCGAA 1 cut(s) 141
BstC8I GCNNGC 1 cut(s) 741
BstDEI CTNAG 5 cut(s) 551, 613, 674, 752, 792
BstF5I GGATG 3 cut(s) 91, 203, 684
BstH2I RGCGCY 1 cut(s) 746
BstHHI GCGC 1 cut(s) 745
BstKTI GATC 3 cut(s) 16, 131, 823
BstMBI GATC 3 cut(s) 13, 128, 820
BstMWI GCNNNNNNNGC 3 cut(s) 475, 526, 679
BstNI CCWGG 2 cut(s) 287, 329
BstSCI CCNGG 2 cut(s) 285, 327
BstSFI CTRYAG 2 cut(s) 171, 191
BstV1I GCAGC 4 cut(s) 35, 157, 205, 691
BstX2I RGATCY 1 cut(s) 128
BstYI RGATCY 1 cut(s) 128
BsuRI GGCC 2 cut(s) 332, 765
BtsCI GGATG 3 cut(s) 91, 203, 684
BtsI GCAGTG 1 cut(s) 606
BtsIMutI CAGTG 2 cut(s) 9, 606
Cac8I GCNNGC 1 cut(s) 741
CaiI CAGNNNCTG 1 cut(s) 670
CfoI GCGC 1 cut(s) 745
CviAII CATG 6 cut(s) 100, 124, 155, 163, 524, 643
DdeI CTNAG 5 cut(s) 551, 613, 674, 752, 792
DpnI GATC 3 cut(s) 15, 130, 822
DpnII GATC 3 cut(s) 13, 128, 820
Eam1104I CTCTTC 3 cut(s) 189, 717, 804
EarI CTCTTC 3 cut(s) 189, 717, 804
Eco147I AGGCCT 1 cut(s) 765
EcoRII CCWGG 2 cut(s) 285, 327
FaeI CATG 6 cut(s) 103, 127, 158, 166, 527, 646
FatI CATG 6 cut(s) 99, 123, 154, 162, 523, 642
FbaI TGATCA 1 cut(s) 820
Fnu4HI GCNGC 5 cut(s) 49, 171, 194, 705, 708
FokI GGATG 3 cut(s) 98, 190, 691
Fsp4HI GCNGC 5 cut(s) 49, 171, 194, 705, 708
FspBI CTAG 1 cut(s) 865
GlaI GCGC 1 cut(s) 744
GluI GCNGC 5 cut(s) 49, 171, 194, 705, 708
GsuI CTGGAG 1 cut(s) 210
HaeII RGCGCY 1 cut(s) 746
HaeIII GGCC 2 cut(s) 332, 765
HhaI GCGC 1 cut(s) 745
Hin1II CATG 6 cut(s) 103, 127, 158, 166, 527, 646
Hin6I GCGC 1 cut(s) 743
HinP1I GCGC 1 cut(s) 743
HindIII AAGCTT 1 cut(s) 548
HinfI GANTC 2 cut(s) 667, 725
HphI GGTGA 1 cut(s) 401
Hpy188I TCNGA 6 cut(s) 72, 271, 666, 755, 795, 820
Hpy188III TCNNGA 2 cut(s) 623, 837
HpyAV CCTTC 1 cut(s) 29
HpyCH4III ACNGT 1 cut(s) 107
HpyCH4IV ACGT 1 cut(s) 352
HpyCH4V TGCA 6 cut(s) 89, 123, 173, 193, 344, 520
HpyF10VI GCNNNNNNNGC 3 cut(s) 475, 526, 679
HpyF3I CTNAG 5 cut(s) 551, 613, 674, 752, 792
HpySE526I ACGT 1 cut(s) 352
Hsp92II CATG 6 cut(s) 103, 127, 158, 166, 527, 646
HspAI GCGC 1 cut(s) 743
Ksp22I TGATCA 1 cut(s) 820
Kzo9I GATC 3 cut(s) 13, 128, 820
LguI GCTCTTC 1 cut(s) 717
LmnI GCTCC 4 cut(s) 483, 496, 651, 835
Lsp1109I GCAGC 4 cut(s) 35, 157, 205, 691
LweI GCATC 4 cut(s) 76, 478, 529, 669
MaeI CTAG 1 cut(s) 865
MaeII ACGT 1 cut(s) 352
MaeIII GTNAC 4 cut(s) 146, 389, 554, 799
MalI GATC 3 cut(s) 15, 130, 822
MboI GATC 3 cut(s) 13, 128, 820
MboII GAAGA 5 cut(s) 176, 549, 611, 704, 821
MfeI CAATTG 1 cut(s) 357
MflI RGATCY 1 cut(s) 128
MmeI TCCRAC 1 cut(s) 156
MroXI GAANNNNTTC 1 cut(s) 660
MseI TTAA 1 cut(s) 231
MspA1I CMGCKG 1 cut(s) 48
MspR9I CCNGG 2 cut(s) 287, 329
MunI CAATTG 1 cut(s) 357
Mva1269I GAATGC 2 cut(s) 137, 706
MvaI CCWGG 2 cut(s) 287, 329
MwoI GCNNNNNNNGC 3 cut(s) 475, 526, 679
NdeII GATC 3 cut(s) 13, 128, 820
NlaIII CATG 6 cut(s) 103, 127, 158, 166, 527, 646
NlaIV GGNNCC 2 cut(s) 130, 777
NmuCI GTSAC 4 cut(s) 146, 389, 554, 799
NspV TTCGAA 1 cut(s) 141
PceI AGGCCT 1 cut(s) 765
PciSI GCTCTTC 1 cut(s) 717
PctI GAATGC 2 cut(s) 137, 706
PdmI GAANNNNTTC 1 cut(s) 660
PfeI GAWTC 2 cut(s) 667, 725
PflMI CCANNNNNTGG 1 cut(s) 580
PfoI TCCNGGA 1 cut(s) 285
PkrI GCNGC 5 cut(s) 50, 172, 195, 706, 709
Psp6I CCWGG 2 cut(s) 285, 327
PspGI CCWGG 2 cut(s) 285, 327
PspN4I GGNNCC 2 cut(s) 130, 777
PstI CTGCAG 2 cut(s) 175, 195
PstNI CAGNNNCTG 1 cut(s) 670
PsuI RGATCY 1 cut(s) 128
PvuII CAGCTG 1 cut(s) 48
SapI GCTCTTC 1 cut(s) 717
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 5 cut(s) 49, 171, 194, 705, 708
Sau3AI GATC 3 cut(s) 13, 128, 820
ScrFI CCNGG 2 cut(s) 287, 329
SfaNI GCATC 4 cut(s) 76, 478, 529, 669
SfcI CTRYAG 2 cut(s) 171, 191
SfuI TTCGAA 1 cut(s) 141
SseBI AGGCCT 1 cut(s) 765
SsiI CCGC 1 cut(s) 707
SspI AATATT 2 cut(s) 61, 464
SspMI CTAG 1 cut(s) 865
StuI AGGCCT 1 cut(s) 765
StyD4I CCNGG 2 cut(s) 285, 327
TaaI ACNGT 1 cut(s) 107
TaiI ACGT 1 cut(s) 355
TaqI TCGA 1 cut(s) 141
TauI GCSGC 1 cut(s) 710
TfiI GAWTC 2 cut(s) 667, 725
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 2 cut(s) 16, 613
TseFI GTSAC 4 cut(s) 146, 389, 554, 799
TseI GCWGC 4 cut(s) 48, 170, 193, 704
Tsp45I GTSAC 4 cut(s) 146, 389, 554, 799
TspDTI ATGAA 2 cut(s) 116, 681
TspGWI ACGGA 1 cut(s) 822
TspRI CASTG 2 cut(s) 16, 613
Van91I CCANNNNNTGG 1 cut(s) 580
XapI RAATTY 1 cut(s) 660
XmnI GAANNNNTTC 1 cut(s) 660
XspI CTAG 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.