pycom07g18140

helix loop helix domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002277
Physical Location & Seq
Reverse (-)
20637817 .. 20640452
2636 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g18140.2

Sequence Viewer

Length: 834 bp
ATGAGAAACGCTGATCTTCTTCCATCTCTACCCTCCCATCTTTTCAACTTTGGAAACAATATTGATATTCAGATGACAAATTGGGATGCACAAATGAACATGAACAGAAAATTTGGGTTTTGCATGGGGTCCAAGGCATTCGAAAGTGACAACCAGAATAACATGAGCGCCGGAGACTTTTCTCTTCCCCTGCAGCAGTCATCATCCCTCATTACCTATCAATCTGCAGTTAACTATCCATCGGGTGCATTCAGATATGATGACATTGCCGGGCAGTCAATGTTGCTGGACAAGAAGATTTCAGCTCCCCAATTTCAAGATTGCCAGGCCATTCAAAATGCAAGGAAACGTCCAATTGAGGTTGATGACCTTGGTCGTCTGGTTGGTGACACGACGGCTCTAAACGACTTATACAACAATAAGAGAAACAAGATGACAAACGGTAATTCTCAACAACAGTGGCATCAAGAAAACTCAATGGAGCAACAAAACAATAAGTTGCATCATCTGCCAGTGAGAAGAAGCCAAAAGCTTAGTGACAAAATCACAGCTCTCCAAAAATTGGTTTCCCCCTATGGCAAGACTGACACTGCCTCAGTTCTTCAAGAGGCTTCCATATATATCATGCTCCTCCAAGAACAAATTCAGAATCTGGAAAGGATGCTTAGTAGTTCATACAAGAATGCTGCAGCTCTTCACCCACAGGAATCTGGGCGTGGGCAGGTGCTTGATCTCAGAAGCAAAGGCCTTTGCTTGGTTCCTGTGTCAATTACTCAGAAAGTGACTATGGAAGCGCGATCGATCATCTTGCTACATCAAGAAAAGTTGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
No domains found.

Protein Analysis

278

Amino Acids

31.24

Weight (kDa)

8.68

Isoelectric Point (pI)

56.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 712
Acc36I ACCTGC 1 cut(s) 712
AccB7I CCANNNNNTGG 1 cut(s) 562
AccII CGCG 1 cut(s) 796
AcsI RAATTY 2 cut(s) 110, 642
AfiI CCNNNNNNNGG 2 cut(s) 562, 754
AgsI TTSAA 4 cut(s) 46, 317, 335, 605
AjnI CCWGG 1 cut(s) 324
AjuI GAANNNNNNNTTGG 2 cut(s) 627, 659
AluBI AGCT 4 cut(s) 305, 532, 551, 692
AluI AGCT 4 cut(s) 305, 532, 551, 692
Alw26I GTCTC 1 cut(s) 168
AlwNI CAGNNNCTG 1 cut(s) 652
AoxI GGCC 2 cut(s) 327, 745
ApeKI GCWGC 3 cut(s) 193, 686, 689
ApoI RAATTY 2 cut(s) 110, 642
Asp700I GAANNNNTTC 1 cut(s) 642
AspLEI GCGC 2 cut(s) 170, 796
AspS9I GGNCC 1 cut(s) 129
AsuC2I CCSGG 1 cut(s) 271
AsuHPI GGTGA 2 cut(s) 398, 689
AsuII TTCGAA 1 cut(s) 141
AvaII GGWCC 1 cut(s) 129
BbvI GCAGC 3 cut(s) 205, 673, 701
BccI CCATC 3 cut(s) 31, 45, 247
BceAI ACGGC 1 cut(s) 411
BcgI CGANNNNNNTGC 2 cut(s) 790, 824
BciT130I CCWGG 1 cut(s) 326
BcnI CCSGG 1 cut(s) 271
BcoDI GTCTC 1 cut(s) 168
BfmI CTRYAG 3 cut(s) 191, 225, 687
BfoI RGCGCY 1 cut(s) 171
BfuAI ACCTGC 1 cut(s) 712
BisI GCNGC 3 cut(s) 194, 687, 690
BlsI GCNGC 3 cut(s) 195, 688, 691
Bme1390I CCNGG 2 cut(s) 271, 326
Bme18I GGWCC 1 cut(s) 129
BmgT120I GGNCC 1 cut(s) 129
BmiI GGNNCC 2 cut(s) 130, 759
BmrFI CCNGG 2 cut(s) 271, 326
BmsI GCATC 4 cut(s) 76, 472, 511, 651
BoxI GACNNNNGTC 1 cut(s) 372
Bpu14I TTCGAA 1 cut(s) 141
BpuMI CCSGG 1 cut(s) 271
Bsa29I ATCGAT 1 cut(s) 800
BsaJI CCNNGG 2 cut(s) 132, 370
Bsc4I CCNNNNNNNGG 2 cut(s) 562, 754
Bse1I ACTGG 1 cut(s) 512
Bse3DI GCAATG 1 cut(s) 264
BseBI CCWGG 1 cut(s) 326
BseCI ATCGAT 1 cut(s) 800
BseDI CCNNGG 2 cut(s) 132, 370
BseGI GGATG 3 cut(s) 91, 203, 666
BseLI CCNNNNNNNGG 2 cut(s) 562, 754
BseMI GCAATG 1 cut(s) 264
BseMII CTCAG 3 cut(s) 609, 748, 788
BseNI ACTGG 1 cut(s) 512
BseRI GAGGAG 1 cut(s) 620
BseXI GCAGC 3 cut(s) 205, 673, 701
Bsh1236I CGCG 1 cut(s) 796
Bsh1285I CGRYCG 1 cut(s) 800
BshFI GGCC 2 cut(s) 329, 747
BshVI ATCGAT 1 cut(s) 800
BsiEI CGRYCG 1 cut(s) 800
BsiSI CCGG 2 cut(s) 171, 270
BslI CCNNNNNNNGG 2 cut(s) 562, 754
BsmAI GTCTC 1 cut(s) 168
BsmI GAATGC 3 cut(s) 137, 248, 688
BsnI GGCC 2 cut(s) 329, 747
Bsp119I TTCGAA 1 cut(s) 141
Bsp143I GATC 4 cut(s) 13, 730, 797, 801
BspANI GGCC 2 cut(s) 329, 747
BspCNI CTCAG 3 cut(s) 608, 747, 787
BspDI ATCGAT 1 cut(s) 800
BspFNI CGCG 1 cut(s) 796
BspLI GGNNCC 2 cut(s) 130, 759
BspMAI CTGCAG 3 cut(s) 195, 229, 691
BspMI ACCTGC 1 cut(s) 712
BspQI GCTCTTC 1 cut(s) 699
BspT104I TTCGAA 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 264
BsrI ACTGG 1 cut(s) 512
BssECI CCNNGG 2 cut(s) 132, 370
BssMI GATC 4 cut(s) 13, 730, 797, 801
BssT1I CCWWGG 2 cut(s) 132, 370
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 2 cut(s) 443, 459
Bst6I CTCTTC 2 cut(s) 189, 699
BstAPI GCANNNNNTGC 1 cut(s) 508
BstBI TTCGAA 1 cut(s) 141
BstDEI CTNAG 5 cut(s) 533, 595, 665, 734, 774
BstF5I GGATG 3 cut(s) 91, 203, 666
BstFNI CGCG 1 cut(s) 796
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 2 cut(s) 170, 796
BstKTI GATC 4 cut(s) 16, 733, 800, 804
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 4 cut(s) 13, 730, 797, 801
BstMCI CGRYCG 1 cut(s) 800
BstMWI GCNNNNNNNGC 1 cut(s) 508
BstNI CCWGG 1 cut(s) 326
BstPAI GACNNNNGTC 1 cut(s) 372
BstSCI CCNGG 2 cut(s) 269, 324
BstSFI CTRYAG 3 cut(s) 191, 225, 687
BstUI CGCG 1 cut(s) 796
BstV1I GCAGC 3 cut(s) 205, 673, 701
Bsu15I ATCGAT 1 cut(s) 800
BsuRI GGCC 2 cut(s) 329, 747
BsuTUI ATCGAT 1 cut(s) 800
BtsCI GGATG 3 cut(s) 91, 203, 666
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 3 cut(s) 464, 519, 588
BveI ACCTGC 1 cut(s) 712
CaiI CAGNNNCTG 1 cut(s) 652
CfoI GCGC 2 cut(s) 170, 796
Cfr13I GGNCC 1 cut(s) 129
ClaI ATCGAT 1 cut(s) 800
CspCI CAANNNNNGTGG 2 cut(s) 440, 475
CviAII CATG 4 cut(s) 100, 124, 163, 625
CviJI RGCY 9 cut(s) 305, 329, 398, 525, 532, 551, 611, 692, 747
CviKI_1 RGCY 9 cut(s) 305, 329, 398, 525, 532, 551, 611, 692, 747
DdeI CTNAG 5 cut(s) 533, 595, 665, 734, 774
DpnI GATC 4 cut(s) 15, 732, 799, 803
DpnII GATC 4 cut(s) 13, 730, 797, 801
Eam1104I CTCTTC 2 cut(s) 189, 699
EarI CTCTTC 2 cut(s) 189, 699
Eco130I CCWWGG 2 cut(s) 132, 370
Eco147I AGGCCT 1 cut(s) 747
Eco47I GGWCC 1 cut(s) 129
EcoRII CCWGG 1 cut(s) 324
EcoT14I CCWWGG 2 cut(s) 132, 370
ErhI CCWWGG 2 cut(s) 132, 370
FaeI CATG 4 cut(s) 103, 127, 166, 628
FatI CATG 4 cut(s) 99, 123, 162, 624
Fnu4HI GCNGC 3 cut(s) 194, 687, 690
FokI GGATG 3 cut(s) 98, 190, 673
Fsp4HI GCNGC 3 cut(s) 194, 687, 690
GlaI GCGC 2 cut(s) 169, 795
GluI GCNGC 3 cut(s) 194, 687, 690
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 2 cut(s) 329, 747
HapII CCGG 2 cut(s) 171, 270
HhaI GCGC 2 cut(s) 170, 796
Hin1II CATG 4 cut(s) 103, 127, 166, 628
Hin6I GCGC 2 cut(s) 168, 794
HinP1I GCGC 2 cut(s) 168, 794
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 2 cut(s) 649, 707
HpaI GTTAAC 1 cut(s) 232
HpaII CCGG 2 cut(s) 171, 270
HphI GGTGA 2 cut(s) 398, 689
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 5 cut(s) 72, 254, 648, 737, 777
Hpy188III TCNNGA 5 cut(s) 317, 467, 605, 653, 818
Hpy8I GTNNAC 1 cut(s) 232
Hpy99I CGWCG 1 cut(s) 397
HpyCH4III ACNGT 2 cut(s) 443, 459
HpyCH4IV ACGT 1 cut(s) 349
HpyCH4V TGCA 8 cut(s) 89, 123, 193, 227, 248, 341, 502, 689
HpyF10VI GCNNNNNNNGC 1 cut(s) 508
HpyF3I CTNAG 5 cut(s) 533, 595, 665, 734, 774
HpySE526I ACGT 1 cut(s) 349
Hsp92II CATG 4 cut(s) 103, 127, 166, 628
HspAI GCGC 2 cut(s) 168, 794
KspAI GTTAAC 1 cut(s) 232
Kzo9I GATC 4 cut(s) 13, 730, 797, 801
LguI GCTCTTC 1 cut(s) 699
LmnI GCTCC 3 cut(s) 310, 481, 633
Lsp1109I GCAGC 3 cut(s) 205, 673, 701
LweI GCATC 4 cut(s) 76, 472, 511, 651
MaeII ACGT 1 cut(s) 349
MaeIII GTNAC 4 cut(s) 146, 386, 536, 781
MalI GATC 4 cut(s) 15, 732, 799, 803
MboI GATC 4 cut(s) 13, 730, 797, 801
MboII GAAGA 7 cut(s) 8, 11, 176, 307, 531, 593, 686
MfeI CAATTG 1 cut(s) 354
MluCI AATT 8 cut(s) 79, 110, 311, 354, 445, 560, 642, 768
MnlI CCTC 6 cut(s) 43, 218, 352, 601, 604, 641
MroXI GAANNNNTTC 1 cut(s) 642
MseI TTAA 1 cut(s) 231
MspI CCGG 2 cut(s) 171, 270
MspR9I CCNGG 2 cut(s) 271, 326
MunI CAATTG 1 cut(s) 354
Mva1269I GAATGC 3 cut(s) 137, 248, 688
MvaI CCWGG 1 cut(s) 326
MvnI CGCG 1 cut(s) 796
MwoI GCNNNNNNNGC 1 cut(s) 508
NciI CCSGG 1 cut(s) 271
NdeII GATC 4 cut(s) 13, 730, 797, 801
NlaIII CATG 4 cut(s) 103, 127, 166, 628
NlaIV GGNNCC 2 cut(s) 130, 759
NmuCI GTSAC 4 cut(s) 146, 386, 536, 781
NspV TTCGAA 1 cut(s) 141
PaqCI CACCTGC 1 cut(s) 712
PceI AGGCCT 1 cut(s) 747
PciSI GCTCTTC 1 cut(s) 699
PctI GAATGC 3 cut(s) 137, 248, 688
PdmI GAANNNNTTC 1 cut(s) 642
PfeI GAWTC 2 cut(s) 649, 707
PflMI CCANNNNNTGG 1 cut(s) 562
PkrI GCNGC 3 cut(s) 195, 688, 691
Ple19I CGATCG 1 cut(s) 800
PshAI GACNNNNGTC 1 cut(s) 372
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 2 cut(s) 130, 759
PspPI GGNCC 1 cut(s) 129
PstI CTGCAG 3 cut(s) 195, 229, 691
PstNI CAGNNNCTG 1 cut(s) 652
PvuI CGATCG 1 cut(s) 800
SapI GCTCTTC 1 cut(s) 699
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 3 cut(s) 194, 687, 690
Sau3AI GATC 4 cut(s) 13, 730, 797, 801
Sau96I GGNCC 1 cut(s) 129
ScrFI CCNGG 2 cut(s) 271, 326
SetI ASST 9 cut(s) 218, 307, 352, 363, 372, 534, 553, 694, 726
SfaNI GCATC 4 cut(s) 76, 472, 511, 651
SfcI CTRYAG 3 cut(s) 191, 225, 687
SfuI TTCGAA 1 cut(s) 141
SinI GGWCC 1 cut(s) 129
Sse9I AATT 8 cut(s) 79, 110, 311, 354, 445, 560, 642, 768
SseBI AGGCCT 1 cut(s) 747
SspI AATATT 1 cut(s) 61
StuI AGGCCT 1 cut(s) 747
StyD4I CCNGG 2 cut(s) 269, 324
StyI CCWWGG 2 cut(s) 132, 370
TaaI ACNGT 2 cut(s) 443, 459
TaiI ACGT 1 cut(s) 352
TaqI TCGA 2 cut(s) 141, 800
TasI AATT 8 cut(s) 79, 110, 311, 354, 445, 560, 642, 768
TfiI GAWTC 2 cut(s) 649, 707
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 3 cut(s) 464, 519, 595
TseFI GTSAC 4 cut(s) 146, 386, 536, 781
TseI GCWGC 3 cut(s) 193, 686, 689
Tsp45I GTSAC 4 cut(s) 146, 386, 536, 781
TspDTI ATGAA 3 cut(s) 110, 116, 663
TspRI CASTG 3 cut(s) 464, 519, 595
Van91I CCANNNNNTGG 1 cut(s) 562
VpaK11BI GGWCC 1 cut(s) 129
XapI RAATTY 2 cut(s) 110, 642
XmnI GAANNNNTTC 1 cut(s) 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.