Rmu_sc0004288.1_g000017

helix loop helix domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004288.1
OG0002277
Physical Location & Seq
Forward (+)
79852 .. 81801
1950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004288.1_g000017.1.cds

Sequence Viewer

Length: 912 bp
atgttaatggggatatggcattatggcagtggcttttgcatggggtccaaagcaatagaaagtgacaacgaaacaggagacatttctgatgttccttttgatcagtcatcactctttacctatcagacagcagtaacagcaggttattccaatggggaattcagatatgatcatggcatttttcagcacccaatgttgtttgacaagaatattaattcagctacccaatttcaagaggtcatacagaatgcaaggaagcgcccaataattgaggtcaatgatatcttgcccaaaaatgaccttggtggttcaattagtgagagaacaacttcaaatgaatggaaccaaaacaagagaaacaaggggacgaattctcaacaacaatggcataatcaattccaagaagcatctatggaaaaacaagacaataggaagttgcaagttcctgtgaggcgaagccaaaagcttagtgacaaaatcactgctcttcaaaaattggtttccccctatggcaaggtcagcagtttgcagagcactaaaaagttcatatctttaattcccagtaatataggaaatgaaattctgtactttctggaaagcctaattcaggtatggttaaattcgaaattgttgcagactgacactgcctcagttcttcaagaggcttcgatttacatcgtgcttcttcaacaacaaattcagaatctgcatcggatctttagtagttcatacaagaatgctgcaggacttcacacacaagaatctgggagtagtagccaagtgcttgacctcagaagcaatggcctttgtttggttccgatttccatgactcagaaagtgactatggaagagggttttgatgaggatcatctttctacatcaagaaaatttgtcatagctaaccatttctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
No domains found.

Protein Analysis

303

Amino Acids

34.29

Weight (kDa)

6.71

Isoelectric Point (pI)

55.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 131
AclWI GGATC 2 cut(s) 722, 873
AcsI RAATTY 6 cut(s) 158, 370, 579, 619, 696, 887
AfaI GTAC 1 cut(s) 587
AfiI CCNNNNNNNGG 2 cut(s) 607, 811
AgsI TTSAA 6 cut(s) 233, 312, 333, 491, 659, 689
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
AluBI AGCT 3 cut(s) 221, 466, 899
AluI AGCT 3 cut(s) 221, 466, 899
Alw21I GWGCWC 1 cut(s) 536
Alw26I GTCTC 1 cut(s) 72
AlwI GGATC 2 cut(s) 722, 873
AlwNI CAGNNNCTG 1 cut(s) 706
AoxI GGCC 1 cut(s) 802
ApeKI GCWGC 1 cut(s) 740
ApoI RAATTY 6 cut(s) 158, 370, 579, 619, 696, 887
AseI ATTAAT 1 cut(s) 213
Asp700I GAANNNNTTC 1 cut(s) 328
AspLEI GCGC 1 cut(s) 261
AspS9I GGNCC 1 cut(s) 45
AsuII TTCGAA 1 cut(s) 623
AvaII GGWCC 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 536
BbvI GCAGC 1 cut(s) 727
BcgI CGANNNNNNTGC 2 cut(s) 613, 647
BclI TGATCA 2 cut(s) 100, 169
BcoDI GTCTC 1 cut(s) 72
BfaI CTAG 1 cut(s) 910
BfmI CTRYAG 1 cut(s) 741
BfoI RGCGCY 1 cut(s) 262
BfuAI ACCTGC 1 cut(s) 131
BisI GCNGC 1 cut(s) 741
BlsI GCNGC 1 cut(s) 742
Bme18I GGWCC 1 cut(s) 45
BmgT120I GGNCC 1 cut(s) 45
BmiI GGNNCC 3 cut(s) 46, 344, 816
BmrI ACTGGG 1 cut(s) 555
BmsI GCATC 2 cut(s) 416, 718
BmuI ACTGGG 1 cut(s) 555
Bpu14I TTCGAA 1 cut(s) 623
BsaBI GATNNNNATC 2 cut(s) 674, 864
BsaJI CCNNGG 1 cut(s) 301
Bsc4I CCNNNNNNNGG 2 cut(s) 607, 811
Bse1I ACTGG 1 cut(s) 561
Bse3DI GCAATG 1 cut(s) 805
Bse8I GATNNNNATC 2 cut(s) 674, 864
BseDI CCNNGG 1 cut(s) 301
BseJI GATNNNNATC 2 cut(s) 674, 864
BseLI CCNNNNNNNGG 2 cut(s) 607, 811
BseMI GCAATG 1 cut(s) 805
BseMII CTCAG 3 cut(s) 663, 805, 845
BseNI ACTGG 1 cut(s) 561
BseXI GCAGC 1 cut(s) 727
BshFI GGCC 1 cut(s) 804
BsiHKAI GWGCWC 1 cut(s) 536
BslFI GGGAC 1 cut(s) 379
BslI CCNNNNNNNGG 2 cut(s) 607, 811
BsmAI GTCTC 1 cut(s) 72
BsmFI GGGAC 1 cut(s) 379
BsmI GAATGC 2 cut(s) 253, 742
BsnI GGCC 1 cut(s) 804
Bsp119I TTCGAA 1 cut(s) 623
Bsp1286I GDGCHC 1 cut(s) 536
Bsp143I GATC 4 cut(s) 100, 169, 714, 865
BspANI GGCC 1 cut(s) 804
BspCNI CTCAG 3 cut(s) 662, 804, 844
BspLI GGNNCC 3 cut(s) 46, 344, 816
BspMAI CTGCAG 1 cut(s) 745
BspMI ACCTGC 1 cut(s) 131
BspPI GGATC 2 cut(s) 722, 873
BspQI GCTCTTC 1 cut(s) 492
BspT104I TTCGAA 1 cut(s) 623
BsrDI GCAATG 1 cut(s) 805
BsrI ACTGG 1 cut(s) 561
BssECI CCNNGG 1 cut(s) 301
BssMI GATC 4 cut(s) 100, 169, 714, 865
BssT1I CCWWGG 1 cut(s) 301
Bst6I CTCTTC 2 cut(s) 492, 843
BstBI TTCGAA 1 cut(s) 623
BstDEI CTNAG 4 cut(s) 467, 649, 791, 831
BstENI CCTNNNNNAGG 1 cut(s) 605
BstH2I RGCGCY 1 cut(s) 262
BstHHI GCGC 1 cut(s) 261
BstKTI GATC 4 cut(s) 103, 172, 717, 868
BstMAI GTCTC 1 cut(s) 72
BstMBI GATC 4 cut(s) 100, 169, 714, 865
BstMWI GCNNNNNNNGC 2 cut(s) 137, 519
BstSFI CTRYAG 1 cut(s) 741
BstV1I GCAGC 1 cut(s) 727
BstX2I RGATCY 1 cut(s) 714
BstYI RGATCY 1 cut(s) 714
BsuRI GGCC 1 cut(s) 804
BtsI GCAGTG 3 cut(s) 34, 480, 642
BtsIMutI CAGTG 3 cut(s) 34, 480, 642
BveI ACCTGC 1 cut(s) 131
CaiI CAGNNNCTG 1 cut(s) 706
CfoI GCGC 1 cut(s) 261
Cfr13I GGNCC 1 cut(s) 45
Csp6I GTAC 1 cut(s) 586
CviAII CATG 3 cut(s) 40, 173, 826
CviJI RGCY 9 cut(s) 33, 221, 459, 466, 600, 665, 777, 804, 899
CviKI_1 RGCY 9 cut(s) 33, 221, 459, 466, 600, 665, 777, 804, 899
CviQI GTAC 1 cut(s) 586
DdeI CTNAG 4 cut(s) 467, 649, 791, 831
DpnI GATC 4 cut(s) 102, 171, 716, 867
DpnII GATC 4 cut(s) 100, 169, 714, 865
Eam1104I CTCTTC 2 cut(s) 492, 843
EarI CTCTTC 2 cut(s) 492, 843
Eco130I CCWWGG 1 cut(s) 301
Eco32I GATATC 1 cut(s) 283
Eco47I GGWCC 1 cut(s) 45
EcoNI CCTNNNNNAGG 1 cut(s) 605
EcoRI GAATTC 2 cut(s) 158, 370
EcoRV GATATC 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 301
ErhI CCWWGG 1 cut(s) 301
FaeI CATG 3 cut(s) 43, 176, 829
FaqI GGGAC 1 cut(s) 379
FatI CATG 3 cut(s) 39, 172, 825
FbaI TGATCA 2 cut(s) 100, 169
Fnu4HI GCNGC 1 cut(s) 741
Fsp4HI GCNGC 1 cut(s) 741
FspBI CTAG 1 cut(s) 910
GlaI GCGC 1 cut(s) 260
GluI GCNGC 1 cut(s) 741
HaeII RGCGCY 1 cut(s) 262
HaeIII GGCC 1 cut(s) 804
HhaI GCGC 1 cut(s) 261
Hin1II CATG 3 cut(s) 43, 176, 829
Hin6I GCGC 1 cut(s) 259
HinP1I GCGC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 3 cut(s) 703, 761, 829
Hpy188I TCNGA 8 cut(s) 88, 126, 164, 702, 714, 794, 819, 834
Hpy188III TCNNGA 4 cut(s) 233, 593, 659, 882
HpyCH4V TGCA 7 cut(s) 39, 251, 439, 529, 634, 709, 743
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 519
HpyF3I CTNAG 4 cut(s) 467, 649, 791, 831
Hsp92II CATG 3 cut(s) 43, 176, 829
HspAI GCGC 1 cut(s) 259
Ksp22I TGATCA 2 cut(s) 100, 169
Kzo9I GATC 4 cut(s) 100, 169, 714, 865
LguI GCTCTTC 1 cut(s) 492
LpnPI CCDG 8 cut(s) 60, 126, 459, 574, 578, 593, 729, 750
Lsp1109I GCAGC 1 cut(s) 727
LweI GCATC 2 cut(s) 416, 718
MaeI CTAG 1 cut(s) 910
MaeIII GTNAC 4 cut(s) 62, 133, 470, 838
MalI GATC 4 cut(s) 102, 171, 716, 867
MboI GATC 4 cut(s) 100, 169, 714, 865
MboII GAAGA 4 cut(s) 479, 647, 677, 860
MflI RGATCY 1 cut(s) 714
MhlI GDGCHC 1 cut(s) 536
MlyI GAGTC 1 cut(s) 823
MnlI CCTC 8 cut(s) 229, 265, 444, 655, 658, 800, 844, 856
MroXI GAANNNNTTC 1 cut(s) 328
MseI TTAA 4 cut(s) 5, 213, 554, 617
Mva1269I GAATGC 2 cut(s) 253, 742
MwoI GCNNNNNNNGC 2 cut(s) 137, 519
NdeII GATC 4 cut(s) 100, 169, 714, 865
NlaIII CATG 3 cut(s) 43, 176, 829
NlaIV GGNNCC 3 cut(s) 46, 344, 816
NmuCI GTSAC 3 cut(s) 62, 470, 838
NspV TTCGAA 1 cut(s) 623
PciSI GCTCTTC 1 cut(s) 492
PctI GAATGC 2 cut(s) 253, 742
PdmI GAANNNNTTC 1 cut(s) 328
PfeI GAWTC 2 cut(s) 703, 761
PkrI GCNGC 1 cut(s) 742
PleI GAGTC 1 cut(s) 823
PpsI GAGTC 1 cut(s) 823
PshBI ATTAAT 1 cut(s) 213
PspN4I GGNNCC 3 cut(s) 46, 344, 816
PspPI GGNCC 1 cut(s) 45
PstI CTGCAG 1 cut(s) 745
PstNI CAGNNNCTG 1 cut(s) 706
PsuI RGATCY 1 cut(s) 714
RsaI GTAC 1 cut(s) 587
RsaNI GTAC 1 cut(s) 586
SapI GCTCTTC 1 cut(s) 492
SaqAI TTAA 4 cut(s) 5, 213, 554, 617
SatI GCNGC 1 cut(s) 741
Sau3AI GATC 4 cut(s) 100, 169, 714, 865
Sau96I GGNCC 1 cut(s) 45
SchI GAGTC 1 cut(s) 823
SduI GDGCHC 1 cut(s) 536
SfaNI GCATC 2 cut(s) 416, 718
SfcI CTRYAG 1 cut(s) 741
SfuI TTCGAA 1 cut(s) 623
SinI GGWCC 1 cut(s) 45
SspI AATATT 1 cut(s) 211
SspMI CTAG 1 cut(s) 910
StyI CCWWGG 1 cut(s) 301
TaqI TCGA 2 cut(s) 623, 668
TatI WGTACW 1 cut(s) 585
TfiI GAWTC 2 cut(s) 703, 761
Tru1I TTAA 4 cut(s) 5, 213, 554, 617
Tru9I TTAA 4 cut(s) 5, 213, 554, 617
TscAI CASTG 3 cut(s) 34, 487, 649
TseFI GTSAC 3 cut(s) 62, 470, 838
TseI GCWGC 1 cut(s) 740
Tsp45I GTSAC 3 cut(s) 62, 470, 838
TspDTI ATGAA 4 cut(s) 351, 535, 591, 717
TspRI CASTG 3 cut(s) 34, 487, 649
VpaK11BI GGWCC 1 cut(s) 45
VspI ATTAAT 1 cut(s) 213
XagI CCTNNNNNAGG 1 cut(s) 605
XapI RAATTY 6 cut(s) 158, 370, 579, 619, 696, 887
XmnI GAANNNNTTC 1 cut(s) 328
XspI CTAG 1 cut(s) 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.