FvH4_7g25700

Appr-1'-p processing enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004383
Physical Location & Seq
Forward (+)
19618468 .. 19623512
5045 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g25700.t6

Sequence Viewer

Length: 678 bp
ATGGCGAGCTTGGGGCCTAAAGCAGCGAGATTTGTGAGGTCGGCTCCGGCGATCAGTTTGAGGTGTGGAAAAGTTCGGAGATTTGTTAGGGTTTCGGCTATGGCGAGTGGTGAAGGAGCTGTGAGGTTTCCGTTGTCTGATAAAAGCACTCTGGTGATACAAATGGGCGATATCACCAAGTGGTTCATCGACGGCTCCACTGACGCCATTGTTAATCCAGCAAATGAGAGAATGCTTGGAGGTGGTGGTGCAGATGGAGCTATACATAGGGCTGCAGGCCCGGATCTTTTACAGGCATGCTATGGTGTTCCGGAAGCCAGGCCTGGAGTCCGCTGCCCTACCGGAGAGGCGAGGATTACCCCAGGATTTAAGTTGCCTGTGGCTCATGTAATTCACACTGTTGGACCTGTATACCGTCCTGGTAACAACCCTGAAGTCTATCTGAGCTCTGCATACAGGAATAGCTTGAGCATAGCCAAAGAACACAACATTCAGTATGTTGCATTTCCTGCTATATCTTGTGGTGTGTATGGATATCCTTATGATGAAGCTTCCGAAGTGGCTCTATCCACAGTTAAAGAATCTGTGAATGACATAAAGGAGGTGCACTTTGTTCTTTTCTCTGAAGATATTTACAATGTCTGGTTGGACAAGGCAAATAAATTGCTTAAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Macro

Protein Analysis

226

Amino Acids

24.22

Weight (kDa)

8.86

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Macro PF01661 71 - 178 1e-35 Macro domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 411
AccIII TCCGGA 1 cut(s) 310
AciI CCGC 1 cut(s) 331
AclWI GGATC 1 cut(s) 291
AcuI CTGAAG 2 cut(s) 453, 645
AcyI GRCGYC 1 cut(s) 204
AdeI CACNNNGTG 1 cut(s) 180
AjnI CCWGG 4 cut(s) 317, 322, 361, 418
AleI CACNNNNGTG 1 cut(s) 152
AluBI AGCT 7 cut(s) 9, 119, 260, 447, 465, 551, 674
AluI AGCT 7 cut(s) 9, 119, 260, 447, 465, 551, 674
Alw21I GWGCWC 2 cut(s) 449, 609
Alw44I GTGCAC 1 cut(s) 605
AlwI GGATC 1 cut(s) 291
Aor13HI TCCGGA 1 cut(s) 310
AoxI GGCC 3 cut(s) 14, 277, 320
ApaLI GTGCAC 1 cut(s) 605
ApeKI GCWGC 3 cut(s) 23, 272, 333
AspS9I GGNCC 3 cut(s) 14, 278, 404
AsuC2I CCSGG 1 cut(s) 281
AsuHPI GGTGA 3 cut(s) 122, 166, 166
AvaII GGWCC 1 cut(s) 404
BaeGI GKGCMC 1 cut(s) 609
BanII GRGCYC 1 cut(s) 449
Bbv12I GWGCWC 2 cut(s) 449, 609
BbvI GCAGC 3 cut(s) 35, 259, 320
BccI CCATC 1 cut(s) 248
BceAI ACGGC 1 cut(s) 208
BciT130I CCWGG 4 cut(s) 319, 324, 363, 420
BcnI CCSGG 1 cut(s) 281
BfmI CTRYAG 1 cut(s) 273
BisI GCNGC 3 cut(s) 24, 273, 334
BlsI GCNGC 3 cut(s) 25, 274, 335
Bme1390I CCNGG 5 cut(s) 281, 319, 324, 363, 420
Bme18I GGWCC 1 cut(s) 404
BmgT120I GGNCC 3 cut(s) 14, 278, 404
BmiI GGNNCC 3 cut(s) 15, 45, 196
BmrFI CCNGG 5 cut(s) 281, 319, 324, 363, 420
BplI GAGNNNNNCTC 2 cut(s) 28, 60
BpmI CTGGAG 1 cut(s) 345
BpuEI CTTGAG 1 cut(s) 487
BpuMI CCSGG 1 cut(s) 281
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 361
BsaWI WCCGGW 2 cut(s) 310, 341
BseAI TCCGGA 1 cut(s) 310
BseBI CCWGG 4 cut(s) 319, 324, 363, 420
BseDI CCNNGG 1 cut(s) 361
BseMII CTCAG 1 cut(s) 434
BseSI GKGCMC 1 cut(s) 609
BseXI GCAGC 3 cut(s) 35, 259, 320
BsgI GTGCAG 1 cut(s) 270
BshFI GGCC 3 cut(s) 16, 279, 322
BsiHKAI GWGCWC 2 cut(s) 449, 609
BsiSI CCGG 4 cut(s) 47, 281, 311, 342
BsmI GAATGC 1 cut(s) 237
BsnI GGCC 3 cut(s) 16, 279, 322
Bsp1286I GDGCHC 2 cut(s) 449, 609
Bsp13I TCCGGA 1 cut(s) 310
Bsp143I GATC 2 cut(s) 51, 283
BspACI CCGC 1 cut(s) 331
BspANI GGCC 3 cut(s) 16, 279, 322
BspCNI CTCAG 1 cut(s) 435
BspEI TCCGGA 1 cut(s) 310
BspLI GGNNCC 3 cut(s) 15, 45, 196
BspMAI CTGCAG 1 cut(s) 277
BspPI GGATC 1 cut(s) 291
BssECI CCNNGG 1 cut(s) 361
BssMI GATC 2 cut(s) 51, 283
BssNAI GTATAC 1 cut(s) 412
BssNI GRCGYC 1 cut(s) 204
Bst1107I GTATAC 1 cut(s) 412
Bst2UI CCWGG 4 cut(s) 319, 324, 363, 420
Bst4CI ACNGT 3 cut(s) 400, 416, 574
BstACI GRCGYC 1 cut(s) 204
BstAPI GCANNNNNTGC 1 cut(s) 509
BstC8I GCNNGC 3 cut(s) 7, 277, 298
BstDEI CTNAG 2 cut(s) 443, 675
BstKTI GATC 2 cut(s) 54, 286
BstMBI GATC 2 cut(s) 51, 283
BstMWI GCNNNNNNNGC 2 cut(s) 257, 509
BstNI CCWGG 4 cut(s) 319, 324, 363, 420
BstNSI RCATGY 1 cut(s) 300
BstSCI CCNGG 5 cut(s) 279, 317, 322, 361, 418
BstSFI CTRYAG 1 cut(s) 273
BstSLI GKGCMC 1 cut(s) 609
BstV1I GCAGC 3 cut(s) 35, 259, 320
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BstZ17I GTATAC 1 cut(s) 412
BsuRI GGCC 3 cut(s) 16, 279, 322
BtsIMutI CAGTG 2 cut(s) 198, 396
Cac8I GCNNGC 3 cut(s) 7, 277, 298
Cfr13I GGNCC 3 cut(s) 14, 278, 404
CseI GACGC 1 cut(s) 212
CviAII CATG 2 cut(s) 297, 386
DdeI CTNAG 2 cut(s) 443, 675
DpnI GATC 2 cut(s) 53, 285
DpnII GATC 2 cut(s) 51, 283
DraIII CACNNNGTG 1 cut(s) 180
Ecl136II GAGCTC 1 cut(s) 447
Eco147I AGGCCT 1 cut(s) 322
Eco24I GRGCYC 1 cut(s) 449
Eco32I GATATC 2 cut(s) 172, 536
Eco47I GGWCC 1 cut(s) 404
Eco53kI GAGCTC 1 cut(s) 447
Eco57I CTGAAG 2 cut(s) 453, 645
EcoICRI GAGCTC 1 cut(s) 447
EcoO109I RGGNCCY 1 cut(s) 14
EcoRII CCWGG 4 cut(s) 317, 322, 361, 418
EcoRV GATATC 2 cut(s) 172, 536
EcoT38I GRGCYC 1 cut(s) 449
FaeI CATG 2 cut(s) 300, 389
FatI CATG 2 cut(s) 296, 385
FblI GTMKAC 1 cut(s) 411
Fnu4HI GCNGC 3 cut(s) 24, 273, 334
FriOI GRGCYC 1 cut(s) 449
Fsp4HI GCNGC 3 cut(s) 24, 273, 334
GluI GCNGC 3 cut(s) 24, 273, 334
GsuI CTGGAG 1 cut(s) 345
HaeIII GGCC 3 cut(s) 16, 279, 322
HapII CCGG 4 cut(s) 47, 281, 311, 342
HgaI GACGC 1 cut(s) 212
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 2 cut(s) 300, 389
HindIII AAGCTT 2 cut(s) 549, 672
HinfI GANTC 2 cut(s) 327, 581
HpaII CCGG 4 cut(s) 47, 281, 311, 342
HphI GGTGA 3 cut(s) 122, 166, 166
Hpy166II GTNNAC 2 cut(s) 412, 607
Hpy188I TCNGA 5 cut(s) 78, 139, 444, 556, 625
Hpy188III TCNNGA 1 cut(s) 311
Hpy8I GTNNAC 2 cut(s) 412, 607
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 1 cut(s) 107
HpyCH4III ACNGT 3 cut(s) 400, 416, 574
HpyCH4V TGCA 5 cut(s) 251, 275, 452, 503, 607
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 509
HpyF3I CTNAG 2 cut(s) 443, 675
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 2 cut(s) 300, 389
Kpn2I TCCGGA 1 cut(s) 310
Kzo9I GATC 2 cut(s) 51, 283
LmnI GCTCC 4 cut(s) 49, 116, 200, 257
Lsp1109I GCAGC 3 cut(s) 35, 259, 320
MaeIII GTNAC 1 cut(s) 422
MalI GATC 2 cut(s) 53, 285
MboI GATC 2 cut(s) 51, 283
MboII GAAGA 1 cut(s) 638
MflI RGATCY 1 cut(s) 283
MhlI GDGCHC 2 cut(s) 449, 609
MluCI AATT 2 cut(s) 390, 662
MlyI GAGTC 1 cut(s) 336
MmeI TCCRAC 2 cut(s) 382, 627
MnlI CCTC 7 cut(s) 30, 54, 117, 233, 340, 345, 595
MroI TCCGGA 1 cut(s) 310
MseI TTAA 4 cut(s) 213, 369, 576, 669
MslI CAYNNNNRTG 1 cut(s) 152
MspA1I CMGCKG 1 cut(s) 333
MspI CCGG 4 cut(s) 47, 281, 311, 342
MspR9I CCNGG 5 cut(s) 281, 319, 324, 363, 420
Mva1269I GAATGC 1 cut(s) 237
MvaI CCWGG 4 cut(s) 319, 324, 363, 420
MwoI GCNNNNNNNGC 2 cut(s) 257, 509
NciI CCSGG 1 cut(s) 281
NdeII GATC 2 cut(s) 51, 283
NlaIII CATG 2 cut(s) 300, 389
NlaIV GGNNCC 3 cut(s) 15, 45, 196
NspI RCATGY 1 cut(s) 300
OliI CACNNNNGTG 1 cut(s) 152
PaeI GCATGC 1 cut(s) 300
PceI AGGCCT 1 cut(s) 322
PcsI WCGNNNNNNNCGW 2 cut(s) 47, 101
PctI GAATGC 1 cut(s) 237
PfeI GAWTC 1 cut(s) 581
PkrI GCNGC 3 cut(s) 25, 274, 335
PleI GAGTC 1 cut(s) 335
PpsI GAGTC 1 cut(s) 335
Psp124BI GAGCTC 1 cut(s) 449
Psp6I CCWGG 4 cut(s) 317, 322, 361, 418
PspGI CCWGG 4 cut(s) 317, 322, 361, 418
PspN4I GGNNCC 3 cut(s) 15, 45, 196
PspPI GGNCC 3 cut(s) 14, 278, 404
PstI CTGCAG 1 cut(s) 277
PsuI RGATCY 1 cut(s) 283
RseI CAYNNNNRTG 1 cut(s) 152
SacI GAGCTC 1 cut(s) 449
SaqAI TTAA 4 cut(s) 213, 369, 576, 669
SatI GCNGC 3 cut(s) 24, 273, 334
Sau3AI GATC 2 cut(s) 51, 283
Sau96I GGNCC 3 cut(s) 14, 278, 404
SchI GAGTC 1 cut(s) 336
ScrFI CCNGG 5 cut(s) 281, 319, 324, 363, 420
SduI GDGCHC 2 cut(s) 449, 609
SfcI CTRYAG 1 cut(s) 273
SinI GGWCC 1 cut(s) 404
SmiMI CAYNNNNRTG 1 cut(s) 152
SmlI CTYRAG 1 cut(s) 466
SmoI CTYRAG 1 cut(s) 466
SphI GCATGC 1 cut(s) 300
Sse9I AATT 2 cut(s) 390, 662
SseBI AGGCCT 1 cut(s) 322
SsiI CCGC 1 cut(s) 331
SstI GAGCTC 1 cut(s) 449
StuI AGGCCT 1 cut(s) 322
StyD4I CCNGG 5 cut(s) 279, 317, 322, 361, 418
TaaI ACNGT 3 cut(s) 400, 416, 574
TaqI TCGA 1 cut(s) 189
TasI AATT 2 cut(s) 390, 662
TfiI GAWTC 1 cut(s) 581
Tru1I TTAA 4 cut(s) 213, 369, 576, 669
Tru9I TTAA 4 cut(s) 213, 369, 576, 669
TscAI CASTG 2 cut(s) 205, 403
TseI GCWGC 3 cut(s) 23, 272, 333
TspDTI ATGAA 2 cut(s) 175, 561
TspGWI ACGGA 1 cut(s) 120
TspRI CASTG 2 cut(s) 205, 403
VneI GTGCAC 1 cut(s) 605
VpaK11BI GGWCC 1 cut(s) 404
XceI RCATGY 1 cut(s) 300
XmiI GTMKAC 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.