Rh1CG353400

Appr-1'-p processing enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0004383
Physical Location & Seq
Forward (+)
62923228 .. 62930074
6847 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG353400.1

Sequence Viewer

Length: 549 bp
ATGGCGAGCTACCTGTGTAAAGCATCAAGATTTCTGGGCCCAGCTCCGGCGATCAATTCAAGCCGTGGAAAAGTTGGGAGCTTTGTTAGGGTTAGAGCTAGGGTTTCGACGATGGCGAGTGGGGAAGGAGCAGTTCGGTTTCCTTTGTCTGATTCAAGCACTCTCGTGATACAAATGGGGGATATCACCAAGTGGTTCGTCGACGGCTCCACTGACGCCATTGTTAATCCAGCGAATGAGCGAATGCTTGGAGGTGGTGGTGCAGATGGAGCTATACATAGGGCTGCTGGCCCGGATCTTCTACAAGCATGCTATGGTGTTCCGGAAGCCAGGCCAGGAGTCCGCTGCCCCACTGGAGAAGCGAGAATTACCCCAGGGTTTAAGTTGCCTGCGTCTCATGTAATTCACACTGTTGGACCTGTCTACCATGCTGGTAACAACCCTGAAGTCTATCTGAGTTCTGCATACAGGAATAGCTTGAGCATAGCCAAAGCGCACAACATTCAGTATGTAGCATTTCCTGCCATATCTTGCGGTGTGTATGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Macro

Protein Analysis

182

Amino Acids

19.02

Weight (kDa)

9.35

Isoelectric Point (pI)

34.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Macro PF01661 75 - 182 5.1e-37 Macro domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 201, 423
AccIII TCCGGA 1 cut(s) 322
AciI CCGC 2 cut(s) 343, 534
AclWI GGATC 1 cut(s) 303
AcuI CTGAAG 1 cut(s) 465
AcyI GRCGYC 1 cut(s) 216
AdeI CACNNNGTG 1 cut(s) 192
AfiI CCNNNNNNNGG 1 cut(s) 46
AgsI TTSAA 2 cut(s) 60, 156
AjnI CCWGG 3 cut(s) 329, 334, 373
AleI CACNNNNGTG 1 cut(s) 164
AluBI AGCT 6 cut(s) 9, 44, 81, 98, 272, 477
AluI AGCT 6 cut(s) 9, 44, 81, 98, 272, 477
Alw26I GTCTC 1 cut(s) 399
AlwI GGATC 1 cut(s) 303
Aor13HI TCCGGA 1 cut(s) 322
AoxI GGCC 3 cut(s) 37, 289, 332
ApaI GGGCCC 1 cut(s) 41
ApeKI GCWGC 2 cut(s) 284, 345
AspLEI GCGC 1 cut(s) 496
AspS9I GGNCC 4 cut(s) 37, 38, 290, 416
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 178
AvaII GGWCC 1 cut(s) 416
BaeGI GKGCMC 1 cut(s) 41
BanII GRGCYC 1 cut(s) 41
BauI CACGAG 1 cut(s) 164
BbvI GCAGC 2 cut(s) 271, 332
BccI CCATC 2 cut(s) 106, 260
BceAI ACGGC 2 cut(s) 48, 220
BciT130I CCWGG 3 cut(s) 331, 336, 375
BcnI CCSGG 1 cut(s) 293
BcoDI GTCTC 1 cut(s) 399
BfaI CTAG 1 cut(s) 99
BisI GCNGC 2 cut(s) 285, 346
BlsI GCNGC 2 cut(s) 286, 347
Bme1390I CCNGG 4 cut(s) 293, 331, 336, 375
Bme18I GGWCC 1 cut(s) 416
BmgT120I GGNCC 4 cut(s) 37, 38, 290, 416
BmiI GGNNCC 2 cut(s) 39, 208
BmrFI CCNGG 4 cut(s) 293, 331, 336, 375
BmsI GCATC 1 cut(s) 32
BpmI CTGGAG 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 499
BpuMI CCSGG 1 cut(s) 293
BsaHI GRCGYC 1 cut(s) 216
BsaJI CCNNGG 3 cut(s) 64, 373, 374
BsaWI WCCGGW 1 cut(s) 322
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse1I ACTGG 1 cut(s) 358
BseAI TCCGGA 1 cut(s) 322
BseBI CCWGG 3 cut(s) 331, 336, 375
BseDI CCNNGG 3 cut(s) 64, 373, 374
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMII CTCAG 1 cut(s) 446
BseNI ACTGG 1 cut(s) 358
BseSI GKGCMC 1 cut(s) 41
BseXI GCAGC 2 cut(s) 271, 332
BseYI CCCAGC 1 cut(s) 40
BsgI GTGCAG 1 cut(s) 282
BshFI GGCC 3 cut(s) 39, 291, 334
BsiSI CCGG 3 cut(s) 47, 293, 323
BslI CCNNNNNNNGG 1 cut(s) 46
BsmAI GTCTC 1 cut(s) 399
BsmBI CGTCTC 1 cut(s) 399
BsmI GAATGC 1 cut(s) 249
BsnI GGCC 3 cut(s) 39, 291, 334
Bsp120I GGGCCC 1 cut(s) 37
Bsp1286I GDGCHC 1 cut(s) 41
Bsp13I TCCGGA 1 cut(s) 322
Bsp143I GATC 2 cut(s) 51, 295
BspACI CCGC 2 cut(s) 343, 534
BspANI GGCC 3 cut(s) 39, 291, 334
BspCNI CTCAG 1 cut(s) 447
BspEI TCCGGA 1 cut(s) 322
BspLI GGNNCC 2 cut(s) 39, 208
BspPI GGATC 1 cut(s) 303
BsrI ACTGG 1 cut(s) 358
BssECI CCNNGG 3 cut(s) 64, 373, 374
BssMI GATC 2 cut(s) 51, 295
BssNI GRCGYC 1 cut(s) 216
BssSI CACGAG 1 cut(s) 164
Bst2BI CACGAG 1 cut(s) 164
Bst2UI CCWGG 3 cut(s) 331, 336, 375
Bst4CI ACNGT 1 cut(s) 412
BstACI GRCGYC 1 cut(s) 216
BstAPI GCANNNNNTGC 1 cut(s) 521
BstC8I GCNNGC 4 cut(s) 7, 289, 310, 390
BstDEI CTNAG 1 cut(s) 455
BstDSI CCRYGG 1 cut(s) 64
BstHHI GCGC 1 cut(s) 496
BstKTI GATC 2 cut(s) 54, 298
BstMAI GTCTC 1 cut(s) 399
BstMBI GATC 2 cut(s) 51, 295
BstMWI GCNNNNNNNGC 2 cut(s) 269, 521
BstNI CCWGG 3 cut(s) 331, 336, 375
BstNSI RCATGY 1 cut(s) 312
BstSCI CCNGG 4 cut(s) 291, 329, 334, 373
BstSLI GKGCMC 1 cut(s) 41
BstV1I GCAGC 2 cut(s) 271, 332
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BsuRI GGCC 3 cut(s) 39, 291, 334
BtgI CCRYGG 1 cut(s) 64
BtsIMutI CAGTG 3 cut(s) 210, 351, 408
Cac8I GCNNGC 4 cut(s) 7, 289, 310, 390
CfoI GCGC 1 cut(s) 496
Cfr13I GGNCC 4 cut(s) 37, 38, 290, 416
CseI GACGC 2 cut(s) 224, 381
CviAII CATG 3 cut(s) 309, 398, 428
DdeI CTNAG 1 cut(s) 455
DpnI GATC 2 cut(s) 53, 297
DpnII GATC 2 cut(s) 51, 295
DraIII CACNNNGTG 1 cut(s) 192
Eco24I GRGCYC 1 cut(s) 41
Eco32I GATATC 1 cut(s) 184
Eco47I GGWCC 1 cut(s) 416
Eco57I CTGAAG 1 cut(s) 465
EcoRII CCWGG 3 cut(s) 329, 334, 373
EcoRV GATATC 1 cut(s) 184
EcoT38I GRGCYC 1 cut(s) 41
Esp3I CGTCTC 1 cut(s) 399
FaeI CATG 3 cut(s) 312, 401, 431
FatI CATG 3 cut(s) 308, 397, 427
FblI GTMKAC 2 cut(s) 201, 423
Fnu4HI GCNGC 2 cut(s) 285, 346
FriOI GRGCYC 1 cut(s) 41
Fsp4HI GCNGC 2 cut(s) 285, 346
FspBI CTAG 1 cut(s) 99
GlaI GCGC 1 cut(s) 495
GluI GCNGC 2 cut(s) 285, 346
GsaI CCCAGC 1 cut(s) 44
GsuI CTGGAG 1 cut(s) 375
HaeIII GGCC 3 cut(s) 39, 291, 334
HapII CCGG 3 cut(s) 47, 293, 323
HgaI GACGC 2 cut(s) 224, 381
HhaI GCGC 1 cut(s) 496
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 3 cut(s) 312, 401, 431
Hin6I GCGC 1 cut(s) 494
HinP1I GCGC 1 cut(s) 494
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 2 cut(s) 152, 339
HpaII CCGG 3 cut(s) 47, 293, 323
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 2 cut(s) 202, 424
Hpy188I TCNGA 2 cut(s) 151, 456
Hpy188III TCNNGA 3 cut(s) 27, 166, 323
Hpy8I GTNNAC 2 cut(s) 202, 424
Hpy99I CGWCG 3 cut(s) 112, 203, 206
HpyAV CCTTC 1 cut(s) 119
HpyCH4III ACNGT 1 cut(s) 412
HpyCH4V TGCA 2 cut(s) 263, 464
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 521
HpyF3I CTNAG 1 cut(s) 455
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 3 cut(s) 312, 401, 431
HspAI GCGC 1 cut(s) 494
Kpn2I TCCGGA 1 cut(s) 322
Kzo9I GATC 2 cut(s) 51, 295
LmnI GCTCC 5 cut(s) 49, 78, 128, 212, 269
Lsp1109I GCAGC 2 cut(s) 271, 332
LweI GCATC 1 cut(s) 32
MaeI CTAG 1 cut(s) 99
MaeIII GTNAC 1 cut(s) 434
MalI GATC 2 cut(s) 53, 297
MboI GATC 2 cut(s) 51, 295
MboII GAAGA 1 cut(s) 290
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 1 cut(s) 41
MluCI AATT 3 cut(s) 55, 366, 402
MlyI GAGTC 1 cut(s) 348
MmeI TCCRAC 1 cut(s) 394
MnlI CCTC 1 cut(s) 245
MroI TCCGGA 1 cut(s) 322
MseI TTAA 2 cut(s) 225, 381
MslI CAYNNNNRTG 1 cut(s) 164
MspA1I CMGCKG 1 cut(s) 345
MspI CCGG 3 cut(s) 47, 293, 323
MspR9I CCNGG 4 cut(s) 293, 331, 336, 375
Mva1269I GAATGC 1 cut(s) 249
MvaI CCWGG 3 cut(s) 331, 336, 375
MwoI GCNNNNNNNGC 2 cut(s) 269, 521
NciI CCSGG 1 cut(s) 293
NdeII GATC 2 cut(s) 51, 295
NlaIII CATG 3 cut(s) 312, 401, 431
NlaIV GGNNCC 2 cut(s) 39, 208
NspI RCATGY 1 cut(s) 312
OliI CACNNNNGTG 1 cut(s) 164
PaeI GCATGC 1 cut(s) 312
PasI CCCWGGG 1 cut(s) 374
PcsI WCGNNNNNNNCGW 1 cut(s) 113
PctI GAATGC 1 cut(s) 249
PfeI GAWTC 1 cut(s) 152
PkrI GCNGC 2 cut(s) 286, 347
PleI GAGTC 1 cut(s) 347
PpsI GAGTC 1 cut(s) 347
Psp6I CCWGG 3 cut(s) 329, 334, 373
PspFI CCCAGC 1 cut(s) 40
PspGI CCWGG 3 cut(s) 329, 334, 373
PspN4I GGNNCC 2 cut(s) 39, 208
PspOMI GGGCCC 1 cut(s) 37
PspPI GGNCC 4 cut(s) 37, 38, 290, 416
PsuI RGATCY 1 cut(s) 295
RseI CAYNNNNRTG 1 cut(s) 164
SalI GTCGAC 1 cut(s) 200
SaqAI TTAA 2 cut(s) 225, 381
SatI GCNGC 2 cut(s) 285, 346
Sau3AI GATC 2 cut(s) 51, 295
Sau96I GGNCC 4 cut(s) 37, 38, 290, 416
SchI GAGTC 1 cut(s) 348
ScrFI CCNGG 4 cut(s) 293, 331, 336, 375
SduI GDGCHC 1 cut(s) 41
SetI ASST 9 cut(s) 11, 15, 46, 83, 100, 256, 274, 421, 479
SfaNI GCATC 1 cut(s) 32
SgrDI CGTCGACG 1 cut(s) 200
SinI GGWCC 1 cut(s) 416
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 478
SmoI CTYRAG 1 cut(s) 478
SphI GCATGC 1 cut(s) 312
Sse9I AATT 3 cut(s) 55, 366, 402
SsiI CCGC 2 cut(s) 343, 534
SspMI CTAG 1 cut(s) 99
StyD4I CCNGG 4 cut(s) 291, 329, 334, 373
TaaI ACNGT 1 cut(s) 412
TaqI TCGA 2 cut(s) 107, 201
TasI AATT 3 cut(s) 55, 366, 402
TfiI GAWTC 1 cut(s) 152
Tru1I TTAA 2 cut(s) 225, 381
Tru9I TTAA 2 cut(s) 225, 381
TscAI CASTG 3 cut(s) 217, 358, 415
TseI GCWGC 2 cut(s) 284, 345
TspRI CASTG 3 cut(s) 217, 358, 415
VpaK11BI GGWCC 1 cut(s) 416
XceI RCATGY 1 cut(s) 312
XmiI GTMKAC 2 cut(s) 201, 423
XspI CTAG 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.