RLG00000026937

Appr-1'-p processing enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004383
Physical Location & Seq
Reverse (-)
7102833 .. 7103889
1057 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026937

Sequence Viewer

Length: 459 bp
ATGCTTGGAGGTGGTGGTGGAGATGGCGGGCTGCTGGCCCGGATCTTCTACAAGCATGCTATGGTGTCCCCGAAGCCAGGCCAGGAGTCCGCTGCCCCACTGGAGAAGCTAGAATTACCCCAGGGTTTAAGTTGCCTGCCTGCGTCTCATGTAATTCACACTGTTGGACCTGTCTACCGTCCTGGTACCGTCTACCCTCCTGCTAACAACCCTGAAGTTTATCTGAGCTCTGCATATAGGAATAGCTTGAGCATAGCCAAAGCACACACCATTCAGTATGTTGCATTTCCTCCCATATCTTGTGGTCCGTATGGATATCCTTATGATGAAGCTGCCGAAGTGTCTCTATCCACAATTAAGGAATCCGTGAATGACATAAAGGAGGTGCACTCTGTTCATAAATCGGAAGTTATTTACAATGTCTGTTTGGAAAAGGCAGAGAAATTGCTTAAAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Macro

Protein Analysis

153

Amino Acids

16.31

Weight (kDa)

6.58

Isoelectric Point (pI)

42.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Macro PF01661 46 - 114 3.7e-17 Macro domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 185
AccB1I GGYRCC 1 cut(s) 185
AccI GTMKAC 2 cut(s) 174, 192
AciI CCGC 2 cut(s) 27, 90
AclWI GGATC 1 cut(s) 50
AcuI CTGAAG 1 cut(s) 234
AfaI GTAC 1 cut(s) 187
AfiI CCNNNNNNNGG 1 cut(s) 77
AjnI CCWGG 4 cut(s) 76, 81, 120, 181
AluBI AGCT 4 cut(s) 109, 228, 246, 332
AluI AGCT 4 cut(s) 109, 228, 246, 332
Alw21I GWGCWC 2 cut(s) 230, 390
Alw26I GTCTC 2 cut(s) 150, 348
Alw44I GTGCAC 1 cut(s) 386
AlwI GGATC 1 cut(s) 50
AoxI GGCC 2 cut(s) 36, 79
ApaLI GTGCAC 1 cut(s) 386
ApeKI GCWGC 3 cut(s) 31, 92, 332
Asp718I GGTACC 1 cut(s) 185
AspS9I GGNCC 3 cut(s) 37, 167, 305
AsuC2I CCSGG 1 cut(s) 40
AvaII GGWCC 2 cut(s) 167, 305
BaeGI GKGCMC 1 cut(s) 390
BanI GGYRCC 1 cut(s) 185
BanII GRGCYC 1 cut(s) 230
Bbv12I GWGCWC 2 cut(s) 230, 390
BbvI GCAGC 3 cut(s) 18, 79, 319
BccI CCATC 1 cut(s) 17
BciT130I CCWGG 4 cut(s) 78, 83, 122, 183
BcnI CCSGG 1 cut(s) 40
BcoDI GTCTC 2 cut(s) 150, 348
BfaI CTAG 1 cut(s) 110
BisI GCNGC 3 cut(s) 32, 93, 333
BlsI GCNGC 3 cut(s) 33, 94, 334
Bme1390I CCNGG 5 cut(s) 40, 78, 83, 122, 183
Bme18I GGWCC 2 cut(s) 167, 305
BmgT120I GGNCC 3 cut(s) 37, 167, 305
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 5 cut(s) 40, 78, 83, 122, 183
BplI GAGNNNNNCTC 2 cut(s) 374, 406
BpmI CTGGAG 1 cut(s) 122
BpuEI CTTGAG 1 cut(s) 268
BpuMI CCSGG 1 cut(s) 40
BsaJI CCNNGG 2 cut(s) 120, 121
Bsc4I CCNNNNNNNGG 1 cut(s) 77
Bse1I ACTGG 1 cut(s) 105
BseBI CCWGG 4 cut(s) 78, 83, 122, 183
BseDI CCNNGG 2 cut(s) 120, 121
BseLI CCNNNNNNNGG 1 cut(s) 77
BseMII CTCAG 1 cut(s) 215
BseNI ACTGG 1 cut(s) 105
BseSI GKGCMC 1 cut(s) 390
BseXI GCAGC 3 cut(s) 18, 79, 319
BshFI GGCC 2 cut(s) 38, 81
BshNI GGYRCC 1 cut(s) 185
BsiHKAI GWGCWC 2 cut(s) 230, 390
BsiSI CCGG 1 cut(s) 40
BslFI GGGAC 1 cut(s) 52
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 2 cut(s) 150, 348
BsmBI CGTCTC 1 cut(s) 150
BsmFI GGGAC 1 cut(s) 52
BsnI GGCC 2 cut(s) 38, 81
Bsp1286I GDGCHC 2 cut(s) 230, 390
Bsp143I GATC 1 cut(s) 42
BspACI CCGC 2 cut(s) 27, 90
BspANI GGCC 2 cut(s) 38, 81
BspCNI CTCAG 1 cut(s) 216
BspLI GGNNCC 1 cut(s) 187
BspPI GGATC 1 cut(s) 50
BspT107I GGYRCC 1 cut(s) 185
BsrI ACTGG 1 cut(s) 105
BssECI CCNNGG 2 cut(s) 120, 121
BssMI GATC 1 cut(s) 42
Bst2UI CCWGG 4 cut(s) 78, 83, 122, 183
Bst4CI ACNGT 3 cut(s) 163, 179, 190
BstC8I GCNNGC 5 cut(s) 29, 36, 57, 137, 141
BstDEI CTNAG 1 cut(s) 224
BstKTI GATC 1 cut(s) 45
BstMAI GTCTC 2 cut(s) 150, 348
BstMBI GATC 1 cut(s) 42
BstNI CCWGG 4 cut(s) 78, 83, 122, 183
BstNSI RCATGY 1 cut(s) 59
BstSCI CCNGG 5 cut(s) 38, 76, 81, 120, 181
BstSLI GKGCMC 1 cut(s) 390
BstV1I GCAGC 3 cut(s) 18, 79, 319
BstX2I RGATCY 1 cut(s) 42
BstYI RGATCY 1 cut(s) 42
BsuRI GGCC 2 cut(s) 38, 81
BtsIMutI CAGTG 2 cut(s) 98, 159
Cac8I GCNNGC 5 cut(s) 29, 36, 57, 137, 141
Cfr13I GGNCC 3 cut(s) 37, 167, 305
CseI GACGC 1 cut(s) 132
Csp6I GTAC 1 cut(s) 186
CviAII CATG 2 cut(s) 56, 149
CviQI GTAC 1 cut(s) 186
DdeI CTNAG 1 cut(s) 224
DpnI GATC 1 cut(s) 44
DpnII GATC 1 cut(s) 42
Ecl136II GAGCTC 1 cut(s) 228
Eco24I GRGCYC 1 cut(s) 230
Eco32I GATATC 1 cut(s) 317
Eco47I GGWCC 2 cut(s) 167, 305
Eco53kI GAGCTC 1 cut(s) 228
Eco57I CTGAAG 1 cut(s) 234
EcoICRI GAGCTC 1 cut(s) 228
EcoRII CCWGG 4 cut(s) 76, 81, 120, 181
EcoRV GATATC 1 cut(s) 317
EcoT38I GRGCYC 1 cut(s) 230
Esp3I CGTCTC 1 cut(s) 150
FaeI CATG 2 cut(s) 59, 152
FaqI GGGAC 1 cut(s) 52
FatI CATG 2 cut(s) 55, 148
FauI CCCGC 1 cut(s) 20
FblI GTMKAC 2 cut(s) 174, 192
Fnu4HI GCNGC 3 cut(s) 32, 93, 333
FriOI GRGCYC 1 cut(s) 230
Fsp4HI GCNGC 3 cut(s) 32, 93, 333
FspBI CTAG 1 cut(s) 110
GluI GCNGC 3 cut(s) 32, 93, 333
GsuI CTGGAG 1 cut(s) 122
HaeIII GGCC 2 cut(s) 38, 81
HapII CCGG 1 cut(s) 40
HgaI GACGC 1 cut(s) 132
Hin1II CATG 2 cut(s) 59, 152
HinfI GANTC 2 cut(s) 86, 362
HpaII CCGG 1 cut(s) 40
Hpy166II GTNNAC 3 cut(s) 175, 193, 388
Hpy188I TCNGA 2 cut(s) 225, 406
Hpy8I GTNNAC 3 cut(s) 175, 193, 388
HpyCH4III ACNGT 3 cut(s) 163, 179, 190
HpyCH4V TGCA 3 cut(s) 233, 284, 388
HpyF3I CTNAG 1 cut(s) 224
Hsp92II CATG 2 cut(s) 59, 152
KpnI GGTACC 1 cut(s) 189
Kzo9I GATC 1 cut(s) 42
Lsp1109I GCAGC 3 cut(s) 18, 79, 319
MaeI CTAG 1 cut(s) 110
MalI GATC 1 cut(s) 44
MboI GATC 1 cut(s) 42
MboII GAAGA 1 cut(s) 37
MflI RGATCY 1 cut(s) 42
MhlI GDGCHC 2 cut(s) 230, 390
MluCI AATT 4 cut(s) 113, 153, 354, 443
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 1 cut(s) 145
MnlI CCTC 3 cut(s) 207, 300, 376
MseI TTAA 3 cut(s) 128, 357, 450
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 1 cut(s) 40
MspR9I CCNGG 5 cut(s) 40, 78, 83, 122, 183
MvaI CCWGG 4 cut(s) 78, 83, 122, 183
NciI CCSGG 1 cut(s) 40
NdeII GATC 1 cut(s) 42
NlaIII CATG 2 cut(s) 59, 152
NlaIV GGNNCC 1 cut(s) 187
NspI RCATGY 1 cut(s) 59
PaeI GCATGC 1 cut(s) 59
PasI CCCWGGG 1 cut(s) 121
PfeI GAWTC 1 cut(s) 362
PkrI GCNGC 3 cut(s) 33, 94, 334
PleI GAGTC 1 cut(s) 94
PpsI GAGTC 1 cut(s) 94
Psp124BI GAGCTC 1 cut(s) 230
Psp6I CCWGG 4 cut(s) 76, 81, 120, 181
PspGI CCWGG 4 cut(s) 76, 81, 120, 181
PspN4I GGNNCC 1 cut(s) 187
PspPI GGNCC 3 cut(s) 37, 167, 305
PsuI RGATCY 1 cut(s) 42
RsaI GTAC 1 cut(s) 187
RsaNI GTAC 1 cut(s) 186
SacI GAGCTC 1 cut(s) 230
SaqAI TTAA 3 cut(s) 128, 357, 450
SatI GCNGC 3 cut(s) 32, 93, 333
Sau3AI GATC 1 cut(s) 42
Sau96I GGNCC 3 cut(s) 37, 167, 305
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 5 cut(s) 40, 78, 83, 122, 183
SduI GDGCHC 2 cut(s) 230, 390
SetI ASST 7 cut(s) 13, 111, 172, 230, 248, 334, 387
SinI GGWCC 2 cut(s) 167, 305
SmlI CTYRAG 1 cut(s) 247
SmoI CTYRAG 1 cut(s) 247
SphI GCATGC 1 cut(s) 59
Sse9I AATT 4 cut(s) 113, 153, 354, 443
SsiI CCGC 2 cut(s) 27, 90
SspMI CTAG 1 cut(s) 110
SstI GAGCTC 1 cut(s) 230
StyD4I CCNGG 5 cut(s) 38, 76, 81, 120, 181
TaaI ACNGT 3 cut(s) 163, 179, 190
TasI AATT 4 cut(s) 113, 153, 354, 443
TfiI GAWTC 1 cut(s) 362
Tru1I TTAA 3 cut(s) 128, 357, 450
Tru9I TTAA 3 cut(s) 128, 357, 450
TscAI CASTG 2 cut(s) 105, 166
TseI GCWGC 3 cut(s) 31, 92, 332
TspDTI ATGAA 2 cut(s) 342, 386
TspGWI ACGGA 2 cut(s) 297, 355
TspRI CASTG 2 cut(s) 105, 166
VneI GTGCAC 1 cut(s) 386
VpaK11BI GGWCC 2 cut(s) 167, 305
XceI RCATGY 1 cut(s) 59
XmiI GTMKAC 2 cut(s) 174, 192
XspI CTAG 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.