MD01G1164200.v1.1

Appr-1'-p processing enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004383
Physical Location & Seq
Forward (+)
26875560 .. 26877760
2201 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1164200.v1.1.491

Sequence Viewer

Length: 687 bp
ATGCCCAGCTACCTGTGCAGAGCATCTAGATTTCTCAGCTCAGCTCCCACGATCAGACTCGCTTCTTCGAACCCTGCAAGATTTCGCTGCTCTGTTAAGCTTTCGACCATGTCTAATGGAGAAGGAGCTGTTCGCTTCCCCTTATCCGCTTCAAGCACTCTCGTAATCCAAAAGGGAGATATCACCAAGTGGTCCGTCGACGGCTCCACGGACGCCATCGTCAATCCAGCAAATGAGGGGATGCTTGGAGGTGGTGGTGCAGATGGAGCTATACATAGGGCTGCTGGCCCAGATCTTCTGCAAGCATGCTATAGTGTCCCAGAAGTCAGGCCACGTGTTCGCTGTCCCACCGGAGAAGCAAGGATCACCCCGGGTTTTAAGTTGCCTGCATCTCATGTAATTCATACTGTTGGACCCGTGTACCGTTCTGGCAGCAACCCGGAAACCTTTCTGAGTGCTGCGTACAGGAATAGCTTGACCTTAGCCAAATCAAACAACCTTCAGCATATCGCGTTTCCTGCTATTTCTTGTGGTGTGTATGGATATCCGTATGATGAAGCAGCCACAGTATCTATATCCACAATTAAGGAGTTTGCGGATGGCCTAAAGGAGGTGCACTTTGTTCTCTTCGCAGACGATATTTACGATGTTTGGGTGAAAAAGGCCAATAAATTGCTTAAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Macro

Protein Analysis

229

Amino Acids

24.37

Weight (kDa)

9.11

Isoelectric Point (pI)

33.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Macro PF01661 74 - 190 3.9e-40 Macro domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 218
AccI GTMKAC 1 cut(s) 198
AccII CGCG 1 cut(s) 512
AciI CCGC 2 cut(s) 147, 596
AclWI GGATC 1 cut(s) 371
AcuI CTGAAG 1 cut(s) 485
AcvI CACGTG 1 cut(s) 335
AcyI GRCGYC 1 cut(s) 213
AdeI CACNNNGTG 1 cut(s) 189
AfaI GTAC 2 cut(s) 422, 464
AfiI CCNNNNNNNGG 1 cut(s) 610
AflIII ACRYGT 1 cut(s) 334
AgsI TTSAA 1 cut(s) 153
AluBI AGCT 8 cut(s) 9, 39, 44, 100, 128, 269, 474, 683
AluI AGCT 8 cut(s) 9, 39, 44, 100, 128, 269, 474, 683
Alw21I GWGCWC 1 cut(s) 618
Alw44I GTGCAC 1 cut(s) 614
AlwI GGATC 1 cut(s) 371
Ama87I CYCGRG 1 cut(s) 370
AoxI GGCC 4 cut(s) 286, 329, 601, 663
ApaLI GTGCAC 1 cut(s) 614
ApeKI GCWGC 5 cut(s) 87, 281, 432, 458, 560
Asp700I GAANNNNTTC 1 cut(s) 447
AspS9I GGNCC 3 cut(s) 192, 287, 413
AsuC2I CCSGG 3 cut(s) 371, 372, 440
AsuHPI GGTGA 3 cut(s) 175, 358, 667
AsuII TTCGAA 1 cut(s) 68
AvaI CYCGRG 1 cut(s) 370
AvaII GGWCC 2 cut(s) 192, 413
BaeGI GKGCMC 1 cut(s) 618
BaeI ACNNNNGTAYC 2 cut(s) 404, 437
BbrPI CACGTG 1 cut(s) 335
Bbv12I GWGCWC 1 cut(s) 618
BbvI GCAGC 5 cut(s) 74, 268, 444, 445, 572
BccI CCATC 3 cut(s) 224, 257, 593
BceAI ACGGC 1 cut(s) 217
BcnI CCSGG 3 cut(s) 371, 372, 440
BfaI CTAG 1 cut(s) 27
BfmI CTRYAG 1 cut(s) 310
BglII AGATCT 1 cut(s) 292
BisI GCNGC 5 cut(s) 88, 282, 433, 459, 561
BlpI GCTNAGC 1 cut(s) 40
BlsI GCNGC 5 cut(s) 89, 283, 434, 460, 562
Bme1390I CCNGG 3 cut(s) 371, 372, 440
Bme18I GGWCC 2 cut(s) 192, 413
BmeT110I CYCGRG 1 cut(s) 370
BmgT120I GGNCC 3 cut(s) 192, 287, 413
BmiI GGNNCC 2 cut(s) 205, 415
BmrFI CCNGG 3 cut(s) 371, 372, 440
BmsI GCATC 3 cut(s) 32, 231, 398
Bpu10I CCTNAGC 1 cut(s) 481
Bpu1102I GCTNAGC 1 cut(s) 40
Bpu14I TTCGAA 1 cut(s) 68
BpuMI CCSGG 3 cut(s) 371, 372, 440
BsaAI YACGTR 1 cut(s) 335
BsaHI GRCGYC 1 cut(s) 213
BsaJI CCNNGG 3 cut(s) 207, 369, 370
BsaWI WCCGGW 1 cut(s) 350
Bsc4I CCNNNNNNNGG 1 cut(s) 610
BseDI CCNNGG 3 cut(s) 207, 369, 370
BseGI GGATG 2 cut(s) 246, 604
BseLI CCNNNNNNNGG 1 cut(s) 610
BseMII CTCAG 3 cut(s) 49, 54, 443
BseSI GKGCMC 1 cut(s) 618
BseXI GCAGC 5 cut(s) 74, 268, 444, 445, 572
BseYI CCCAGC 1 cut(s) 5
BsgI GTGCAG 2 cut(s) 37, 279
Bsh1236I CGCG 1 cut(s) 512
BshFI GGCC 4 cut(s) 288, 331, 603, 665
BsiHKAI GWGCWC 1 cut(s) 618
BsiHKCI CYCGRG 1 cut(s) 370
BsiSI CCGG 3 cut(s) 351, 371, 440
BslFI GGGAC 2 cut(s) 302, 330
BslI CCNNNNNNNGG 1 cut(s) 610
BsmFI GGGAC 2 cut(s) 302, 330
BsnI GGCC 4 cut(s) 288, 331, 603, 665
BsoBI CYCGRG 1 cut(s) 370
Bsp119I TTCGAA 1 cut(s) 68
Bsp1286I GDGCHC 1 cut(s) 618
Bsp143I GATC 3 cut(s) 51, 292, 363
Bsp1720I GCTNAGC 1 cut(s) 40
BspACI CCGC 2 cut(s) 147, 596
BspANI GGCC 4 cut(s) 288, 331, 603, 665
BspCNI CTCAG 3 cut(s) 48, 53, 444
BspFNI CGCG 1 cut(s) 512
BspLI GGNNCC 2 cut(s) 205, 415
BspPI GGATC 1 cut(s) 371
BspT104I TTCGAA 1 cut(s) 68
BssECI CCNNGG 3 cut(s) 207, 369, 370
BssMI GATC 3 cut(s) 51, 292, 363
BssNI GRCGYC 1 cut(s) 213
Bst4CI ACNGT 3 cut(s) 409, 425, 568
Bst6I CTCTTC 1 cut(s) 632
BstACI GRCGYC 1 cut(s) 213
BstBAI YACGTR 1 cut(s) 335
BstBI TTCGAA 1 cut(s) 68
BstC8I GCNNGC 4 cut(s) 286, 303, 307, 387
BstDEI CTNAG 4 cut(s) 35, 40, 452, 481
BstDSI CCRYGG 1 cut(s) 207
BstENI CCTNNNNNAGG 1 cut(s) 608
BstF5I GGATG 2 cut(s) 246, 604
BstFNI CGCG 1 cut(s) 512
BstKTI GATC 3 cut(s) 54, 295, 366
BstMBI GATC 3 cut(s) 51, 292, 363
BstMWI GCNNNNNNNGC 3 cut(s) 15, 266, 518
BstNSI RCATGY 1 cut(s) 309
BstSCI CCNGG 3 cut(s) 369, 370, 438
BstSFI CTRYAG 1 cut(s) 310
BstSLI GKGCMC 1 cut(s) 618
BstUI CGCG 1 cut(s) 512
BstV1I GCAGC 5 cut(s) 74, 268, 444, 445, 572
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
BsuRI GGCC 4 cut(s) 288, 331, 603, 665
BtgI CCRYGG 1 cut(s) 207
BtsCI GGATG 2 cut(s) 246, 604
Cac8I GCNNGC 4 cut(s) 286, 303, 307, 387
Cfr13I GGNCC 3 cut(s) 192, 287, 413
Cfr9I CCCGGG 1 cut(s) 370
CseI GACGC 1 cut(s) 221
Csp6I GTAC 2 cut(s) 421, 463
CviAII CATG 3 cut(s) 109, 306, 395
CviQI GTAC 2 cut(s) 421, 463
DdeI CTNAG 4 cut(s) 35, 40, 452, 481
DpnI GATC 3 cut(s) 53, 294, 365
DpnII GATC 3 cut(s) 51, 292, 363
DraIII CACNNNGTG 1 cut(s) 189
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
Eam1104I CTCTTC 1 cut(s) 632
EarI CTCTTC 1 cut(s) 632
Eco32I GATATC 2 cut(s) 181, 545
Eco47I GGWCC 2 cut(s) 192, 413
Eco57I CTGAAG 1 cut(s) 485
Eco72I CACGTG 1 cut(s) 335
Eco88I CYCGRG 1 cut(s) 370
EcoNI CCTNNNNNAGG 1 cut(s) 608
EcoRV GATATC 2 cut(s) 181, 545
FaeI CATG 3 cut(s) 112, 309, 398
FaqI GGGAC 2 cut(s) 302, 330
FatI CATG 3 cut(s) 108, 305, 394
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 5 cut(s) 88, 282, 433, 459, 561
FokI GGATG 2 cut(s) 253, 611
Fsp4HI GCNGC 5 cut(s) 88, 282, 433, 459, 561
FspBI CTAG 1 cut(s) 27
GluI GCNGC 5 cut(s) 88, 282, 433, 459, 561
GsaI CCCAGC 1 cut(s) 9
HaeIII GGCC 4 cut(s) 288, 331, 603, 665
HapII CCGG 3 cut(s) 351, 371, 440
HgaI GACGC 1 cut(s) 221
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 3 cut(s) 112, 309, 398
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HindIII AAGCTT 2 cut(s) 98, 681
HinfI GANTC 1 cut(s) 57
HpaII CCGG 3 cut(s) 351, 371, 440
HphI GGTGA 3 cut(s) 175, 358, 667
Hpy166II GTNNAC 3 cut(s) 199, 421, 616
Hpy188I TCNGA 2 cut(s) 56, 453
Hpy188III TCNNGA 1 cut(s) 27
Hpy8I GTNNAC 3 cut(s) 199, 421, 616
Hpy99I CGWCG 2 cut(s) 200, 203
HpyAV CCTTC 2 cut(s) 116, 509
HpyCH4III ACNGT 3 cut(s) 409, 425, 568
HpyCH4IV ACGT 1 cut(s) 334
HpyCH4V TGCA 6 cut(s) 18, 77, 260, 301, 389, 616
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 266, 518
HpyF3I CTNAG 4 cut(s) 35, 40, 452, 481
HpySE526I ACGT 1 cut(s) 334
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 3 cut(s) 112, 309, 398
Kzo9I GATC 3 cut(s) 51, 292, 363
LmnI GCTCC 4 cut(s) 49, 125, 209, 266
Lsp1109I GCAGC 5 cut(s) 74, 268, 444, 445, 572
LweI GCATC 3 cut(s) 32, 231, 398
MaeI CTAG 1 cut(s) 27
MaeII ACGT 1 cut(s) 334
MalI GATC 3 cut(s) 53, 294, 365
MboI GATC 3 cut(s) 51, 292, 363
MboII GAAGA 3 cut(s) 57, 287, 619
MflI RGATCY 1 cut(s) 292
MhlI GDGCHC 1 cut(s) 618
MluCI AATT 3 cut(s) 399, 582, 671
MlyI GAGTC 1 cut(s) 51
MmeI TCCRAC 1 cut(s) 391
MnlI CCTC 3 cut(s) 229, 242, 604
MroXI GAANNNNTTC 1 cut(s) 447
MseI TTAA 5 cut(s) 96, 378, 585, 678, 685
MspI CCGG 3 cut(s) 351, 371, 440
MspR9I CCNGG 3 cut(s) 371, 372, 440
MvnI CGCG 1 cut(s) 512
MwoI GCNNNNNNNGC 3 cut(s) 15, 266, 518
NciI CCSGG 3 cut(s) 371, 372, 440
NdeII GATC 3 cut(s) 51, 292, 363
NlaIII CATG 3 cut(s) 112, 309, 398
NlaIV GGNNCC 2 cut(s) 205, 415
NspI RCATGY 1 cut(s) 309
NspV TTCGAA 1 cut(s) 68
PaeI GCATGC 1 cut(s) 309
PcsI WCGNNNNNNNCGW 1 cut(s) 642
PdmI GAANNNNTTC 1 cut(s) 447
PflFI GACNNNGTC 1 cut(s) 109
PkrI GCNGC 5 cut(s) 89, 283, 434, 460, 562
PleI GAGTC 1 cut(s) 51
PmaCI CACGTG 1 cut(s) 335
PmlI CACGTG 1 cut(s) 335
PpsI GAGTC 1 cut(s) 51
Ppu21I YACGTR 1 cut(s) 335
PspCI CACGTG 1 cut(s) 335
PspFI CCCAGC 1 cut(s) 5
PspN4I GGNNCC 2 cut(s) 205, 415
PspPI GGNCC 3 cut(s) 192, 287, 413
PsuI RGATCY 1 cut(s) 292
PsyI GACNNNGTC 1 cut(s) 109
RsaI GTAC 2 cut(s) 422, 464
RsaNI GTAC 2 cut(s) 421, 463
SalI GTCGAC 1 cut(s) 197
SaqAI TTAA 5 cut(s) 96, 378, 585, 678, 685
SatI GCNGC 5 cut(s) 88, 282, 433, 459, 561
Sau3AI GATC 3 cut(s) 51, 292, 363
Sau96I GGNCC 3 cut(s) 192, 287, 413
SchI GAGTC 1 cut(s) 51
ScrFI CCNGG 3 cut(s) 371, 372, 440
SduI GDGCHC 1 cut(s) 618
SfaNI GCATC 3 cut(s) 32, 231, 398
SfcI CTRYAG 1 cut(s) 310
SfuI TTCGAA 1 cut(s) 68
SgrDI CGTCGACG 1 cut(s) 197
SinI GGWCC 2 cut(s) 192, 413
SmaI CCCGGG 1 cut(s) 372
SphI GCATGC 1 cut(s) 309
Sse9I AATT 3 cut(s) 399, 582, 671
SsiI CCGC 2 cut(s) 147, 596
SspMI CTAG 1 cut(s) 27
StyD4I CCNGG 3 cut(s) 369, 370, 438
TaaI ACNGT 3 cut(s) 409, 425, 568
TaiI ACGT 1 cut(s) 337
TaqI TCGA 3 cut(s) 68, 104, 198
TasI AATT 3 cut(s) 399, 582, 671
Tru1I TTAA 5 cut(s) 96, 378, 585, 678, 685
Tru9I TTAA 5 cut(s) 96, 378, 585, 678, 685
TseI GCWGC 5 cut(s) 87, 281, 432, 458, 560
TspDTI ATGAA 2 cut(s) 392, 570
TspGWI ACGGA 3 cut(s) 184, 224, 537
TspMI CCCGGG 1 cut(s) 370
Tth111I GACNNNGTC 1 cut(s) 109
VneI GTGCAC 1 cut(s) 614
VpaK11BI GGWCC 2 cut(s) 192, 413
XagI CCTNNNNNAGG 1 cut(s) 608
XbaI TCTAGA 1 cut(s) 26
XceI RCATGY 1 cut(s) 309
XmaI CCCGGG 1 cut(s) 370
XmiI GTMKAC 1 cut(s) 198
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.