Rorug01G0367300

Appr-1'-p processing enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0004383
Physical Location & Seq
Forward (+)
48285358 .. 48285840
483 bp
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UTR
Exon/CDS
Intron
Rorug01G0367300.1

Sequence Viewer

Length: 483 bp
ATGCAGCGTTTCAAGATCCTTGCTTGGAATTGTCGAGGCCTCAACAATATTGAAACTCAGGATGCCCTAGTCAATATTGTACGCCAGCATAACCCTAGTATTGTTTTCCTATCTGAAACCCTAGCCACCCTTGATCTGTTGAACGAGGTTCGTAGGCGCATAGGCTTTGATGGTGTCCTTTGCTCACCTCGGGAGGACGACTGTCATGGACTTGGCTTGTTCTGGCGCAACGAGATTCCAGTACGTCTTCGAAATTATTCCGCTAACCACATCGATACAGAAGTCGGCGCTGTGGGTTCAGATGGTGTCTGTCGATTCACCGGGATATATGGAGTAGCCACAGCGGCGAATCGAATGGTTACTTGGAACCTGCTACGAACCCTAGCAGCTCAGATTCGTCTACCCTGGTTGATTGCCGGAGACTTTAATGAAATTCTCTACTTGGAAGACAAGTCTGGTGGCCCATCTCGTTGTGTATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Macro

Protein Analysis

160

Amino Acids

17.97

Weight (kDa)

6.82

Isoelectric Point (pI)

47.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 8 - 144 1.2e-09 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 378
AccI GTMKAC 1 cut(s) 400
AciI CCGC 2 cut(s) 261, 344
AclWI GGATC 1 cut(s) 10
AcsI RAATTY 1 cut(s) 432
AfaI GTAC 2 cut(s) 81, 243
AgsI TTSAA 3 cut(s) 13, 53, 142
AjnI CCWGG 1 cut(s) 404
AjuI GAANNNNNNNTTGG 2 cut(s) 346, 378
AluBI AGCT 1 cut(s) 389
AluI AGCT 1 cut(s) 389
Alw26I GTCTC 1 cut(s) 414
AlwI GGATC 1 cut(s) 10
Ama87I CYCGRG 1 cut(s) 189
AoxI GGCC 2 cut(s) 37, 460
ApeKI GCWGC 2 cut(s) 4, 386
ApoI RAATTY 1 cut(s) 432
Asp700I GAANNNNTTC 1 cut(s) 256
AspLEI GCGC 3 cut(s) 159, 228, 290
AspS9I GGNCC 1 cut(s) 461
AsuC2I CCSGG 1 cut(s) 322
AsuHPI GGTGA 2 cut(s) 177, 310
AsuII TTCGAA 1 cut(s) 250
AvaI CYCGRG 1 cut(s) 189
BaeI ACNNNNGTAYC 2 cut(s) 267, 300
BbsI GAAGAC 2 cut(s) 239, 453
BbvI GCAGC 2 cut(s) 16, 398
BccI CCATC 3 cut(s) 164, 296, 472
BciT130I CCWGG 1 cut(s) 406
BcnI CCSGG 1 cut(s) 322
BcoDI GTCTC 1 cut(s) 414
BfaI CTAG 4 cut(s) 68, 96, 122, 383
BfoI RGCGCY 1 cut(s) 291
BfuAI ACCTGC 1 cut(s) 378
BglI GCCNNNNNGGC 1 cut(s) 344
BisI GCNGC 3 cut(s) 5, 345, 387
BlsI GCNGC 3 cut(s) 6, 346, 388
Bme1390I CCNGG 2 cut(s) 322, 406
BmeT110I CYCGRG 1 cut(s) 189
BmgT120I GGNCC 1 cut(s) 461
BmiI GGNNCC 1 cut(s) 368
BmrFI CCNGG 2 cut(s) 322, 406
BmsI GCATC 1 cut(s) 52
BoxI GACNNNNGTC 1 cut(s) 201
BpiI GAAGAC 2 cut(s) 239, 453
Bpu14I TTCGAA 1 cut(s) 250
BpuMI CCSGG 1 cut(s) 322
Bsa29I ATCGAT 1 cut(s) 273
BsaJI CCNNGG 2 cut(s) 188, 404
Bse1I ACTGG 1 cut(s) 239
BseBI CCWGG 1 cut(s) 406
BseCI ATCGAT 1 cut(s) 273
BseDI CCNNGG 2 cut(s) 188, 404
BseGI GGATG 1 cut(s) 67
BseMII CTCAG 2 cut(s) 71, 404
BseNI ACTGG 1 cut(s) 239
BseXI GCAGC 2 cut(s) 16, 398
BshFI GGCC 2 cut(s) 39, 462
BshVI ATCGAT 1 cut(s) 273
BsiHKCI CYCGRG 1 cut(s) 189
BsiSI CCGG 2 cut(s) 321, 417
BsmAI GTCTC 1 cut(s) 414
BsnI GGCC 2 cut(s) 39, 462
BsoBI CYCGRG 1 cut(s) 189
Bsp119I TTCGAA 1 cut(s) 250
Bsp143I GATC 2 cut(s) 15, 133
BspACI CCGC 2 cut(s) 261, 344
BspANI GGCC 2 cut(s) 39, 462
BspCNI CTCAG 2 cut(s) 70, 403
BspDI ATCGAT 1 cut(s) 273
BspLI GGNNCC 1 cut(s) 368
BspMI ACCTGC 1 cut(s) 378
BspPI GGATC 1 cut(s) 10
BspT104I TTCGAA 1 cut(s) 250
BsrI ACTGG 1 cut(s) 239
BssECI CCNNGG 2 cut(s) 188, 404
BssMI GATC 2 cut(s) 15, 133
Bst2UI CCWGG 1 cut(s) 406
Bst4CI ACNGT 1 cut(s) 203
BstBI TTCGAA 1 cut(s) 250
BstC8I GCNNGC 1 cut(s) 86
BstDEI CTNAG 2 cut(s) 57, 390
BstF5I GGATG 1 cut(s) 67
BstH2I RGCGCY 1 cut(s) 291
BstHHI GCGC 3 cut(s) 159, 228, 290
BstKTI GATC 2 cut(s) 18, 136
BstMAI GTCTC 1 cut(s) 414
BstMBI GATC 2 cut(s) 15, 133
BstMWI GCNNNNNNNGC 1 cut(s) 344
BstNI CCWGG 1 cut(s) 406
BstPAI GACNNNNGTC 1 cut(s) 201
BstSCI CCNGG 2 cut(s) 320, 404
BstV1I GCAGC 2 cut(s) 16, 398
BstV2I GAAGAC 2 cut(s) 239, 453
BstX2I RGATCY 1 cut(s) 15
BstYI RGATCY 1 cut(s) 15
Bsu15I ATCGAT 1 cut(s) 273
BsuRI GGCC 2 cut(s) 39, 462
BsuTUI ATCGAT 1 cut(s) 273
BtsCI GGATG 1 cut(s) 67
BveI ACCTGC 1 cut(s) 378
Cac8I GCNNGC 1 cut(s) 86
CfoI GCGC 3 cut(s) 159, 228, 290
Cfr13I GGNCC 1 cut(s) 461
ClaI ATCGAT 1 cut(s) 273
Csp6I GTAC 2 cut(s) 80, 242
CspCI CAANNNNNGTGG 2 cut(s) 439, 474
CviAII CATG 1 cut(s) 206
CviJI RGCY 7 cut(s) 39, 125, 165, 216, 338, 389, 462
CviKI_1 RGCY 7 cut(s) 39, 125, 165, 216, 338, 389, 462
CviQI GTAC 2 cut(s) 80, 242
DdeI CTNAG 2 cut(s) 57, 390
DpnI GATC 2 cut(s) 17, 135
DpnII GATC 2 cut(s) 15, 133
Eco147I AGGCCT 1 cut(s) 39
Eco88I CYCGRG 1 cut(s) 189
EcoRII CCWGG 1 cut(s) 404
FaeI CATG 1 cut(s) 209
FaiI YATR 6 cut(s) 90, 161, 207, 328, 330, 481
FatI CATG 1 cut(s) 205
FblI GTMKAC 1 cut(s) 400
Fnu4HI GCNGC 3 cut(s) 5, 345, 387
FokI GGATG 1 cut(s) 74
Fsp4HI GCNGC 3 cut(s) 5, 345, 387
FspBI CTAG 4 cut(s) 68, 96, 122, 383
GlaI GCGC 3 cut(s) 158, 227, 289
GluI GCNGC 3 cut(s) 5, 345, 387
HaeII RGCGCY 1 cut(s) 291
HaeIII GGCC 2 cut(s) 39, 462
HapII CCGG 2 cut(s) 321, 417
HhaI GCGC 3 cut(s) 159, 228, 290
Hin1II CATG 1 cut(s) 209
Hin6I GCGC 3 cut(s) 157, 226, 288
HinP1I GCGC 3 cut(s) 157, 226, 288
HinfI GANTC 4 cut(s) 235, 315, 349, 394
HpaII CCGG 2 cut(s) 321, 417
HphI GGTGA 2 cut(s) 177, 310
Hpy166II GTNNAC 1 cut(s) 401
Hpy188I TCNGA 3 cut(s) 115, 301, 393
Hpy188III TCNNGA 3 cut(s) 13, 59, 191
Hpy8I GTNNAC 1 cut(s) 401
HpyCH4III ACNGT 1 cut(s) 203
HpyCH4IV ACGT 1 cut(s) 244
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 344
HpyF3I CTNAG 2 cut(s) 57, 390
HpySE526I ACGT 1 cut(s) 244
Hsp92II CATG 1 cut(s) 209
HspAI GCGC 3 cut(s) 157, 226, 288
Kzo9I GATC 2 cut(s) 15, 133
Lsp1109I GCAGC 2 cut(s) 16, 398
LweI GCATC 1 cut(s) 52
MaeI CTAG 4 cut(s) 68, 96, 122, 383
MaeII ACGT 1 cut(s) 244
MaeIII GTNAC 1 cut(s) 358
MalI GATC 2 cut(s) 17, 135
MboI GATC 2 cut(s) 15, 133
MboII GAAGA 2 cut(s) 239, 458
MflI RGATCY 1 cut(s) 15
MluCI AATT 3 cut(s) 28, 253, 432
MnlI CCTC 5 cut(s) 29, 50, 139, 187, 198
MroXI GAANNNNTTC 1 cut(s) 256
MseI TTAA 1 cut(s) 426
MspA1I CMGCKG 1 cut(s) 344
MspI CCGG 2 cut(s) 321, 417
MspR9I CCNGG 2 cut(s) 322, 406
MvaI CCWGG 1 cut(s) 406
MwoI GCNNNNNNNGC 1 cut(s) 344
NciI CCSGG 1 cut(s) 322
NdeII GATC 2 cut(s) 15, 133
NlaIII CATG 1 cut(s) 209
NlaIV GGNNCC 1 cut(s) 368
NspV TTCGAA 1 cut(s) 250
PceI AGGCCT 1 cut(s) 39
PdmI GAANNNNTTC 1 cut(s) 256
PfeI GAWTC 4 cut(s) 235, 315, 349, 394
PkrI GCNGC 3 cut(s) 6, 346, 388
PshAI GACNNNNGTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 404
PspGI CCWGG 1 cut(s) 404
PspN4I GGNNCC 1 cut(s) 368
PspPI GGNCC 1 cut(s) 461
PsuI RGATCY 1 cut(s) 15
RsaI GTAC 2 cut(s) 81, 243
RsaNI GTAC 2 cut(s) 80, 242
SaqAI TTAA 1 cut(s) 426
SatI GCNGC 3 cut(s) 5, 345, 387
Sau3AI GATC 2 cut(s) 15, 133
Sau96I GGNCC 1 cut(s) 461
ScrFI CCNGG 2 cut(s) 322, 406
SetI ASST 5 cut(s) 150, 190, 247, 372, 391
SfaNI GCATC 1 cut(s) 52
SfuI TTCGAA 1 cut(s) 250
Sse9I AATT 3 cut(s) 28, 253, 432
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 2 cut(s) 261, 344
SspI AATATT 2 cut(s) 49, 76
SspMI CTAG 4 cut(s) 68, 96, 122, 383
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 320, 404
TaaI ACNGT 1 cut(s) 203
TaiI ACGT 1 cut(s) 247
TaqI TCGA 5 cut(s) 34, 250, 273, 313, 352
TasI AATT 3 cut(s) 28, 253, 432
TauI GCSGC 1 cut(s) 347
TfiI GAWTC 4 cut(s) 235, 315, 349, 394
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TseI GCWGC 2 cut(s) 4, 386
TspDTI ATGAA 1 cut(s) 444
XapI RAATTY 1 cut(s) 432
XmiI GTMKAC 1 cut(s) 400
XmnI GAANNNNTTC 1 cut(s) 256
XspI CTAG 4 cut(s) 68, 96, 122, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.