Rw1G033160

Appr-1'-p processing enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0004383
Physical Location & Seq
Forward (+)
61304058 .. 61305132
1075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G033160.1

Sequence Viewer

Length: 450 bp
ATGGTGTCCCCGAAGCCAGGCCAGGCGTCCGCTGCCCCACTGGAGAAGCTAGAATTACCCTTGACCAACCGCATTGTAATTCACACTGTTGGACCTGTCTACCGTCCTGGTAGCGTCTACCCTCCTGCTAACAACCCTGAAGTTTATCTGAGCTCTGCGTATAGGAATAGCTTGAGCATAGCCAAAGCACACAACATTCAGTATGTTGCGTTTCCTCCCATATCTTGTGGTTCGTATGGATATCCTTATGATGAAGCTGCCGAAGTGTCTCTGTCCACAATCAAGGAATCCGTGAATGACATAAAGGAGGTGCACTTTGTTCATAAATCGGAAGTTATTTACAATGTCTGGTTGGAAAAGGCAAGAAATTGCTTAAAGCCTAATATGTACAAGGAACGAGGAATAAGGAGCTTGCAGATGTATAAGCCGTTCTGTCAGTTTGCAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Macro

Protein Analysis

149

Amino Acids

16.77

Weight (kDa)

8.93

Isoelectric Point (pI)

38.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Macro PF01661 20 - 89 2.8e-17 Macro domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 99, 117
AciI CCGC 2 cut(s) 30, 70
AcuI CTGAAG 1 cut(s) 159
AcyI GRCGYC 1 cut(s) 26
AfaI GTAC 1 cut(s) 389
AfiI CCNNNNNNNGG 1 cut(s) 17
AjnI CCWGG 3 cut(s) 16, 21, 106
AluBI AGCT 5 cut(s) 49, 153, 171, 257, 411
AluI AGCT 5 cut(s) 49, 153, 171, 257, 411
Alw21I GWGCWC 2 cut(s) 155, 315
Alw26I GTCTC 1 cut(s) 273
Alw44I GTGCAC 1 cut(s) 311
AoxI GGCC 1 cut(s) 19
ApaLI GTGCAC 1 cut(s) 311
ApeKI GCWGC 2 cut(s) 32, 257
AspS9I GGNCC 1 cut(s) 92
AvaII GGWCC 1 cut(s) 92
BaeGI GKGCMC 1 cut(s) 315
BanII GRGCYC 1 cut(s) 155
Bbv12I GWGCWC 2 cut(s) 155, 315
BbvI GCAGC 2 cut(s) 19, 244
BceAI ACGGC 1 cut(s) 412
BciT130I CCWGG 3 cut(s) 18, 23, 108
BcoDI GTCTC 1 cut(s) 273
BfaI CTAG 1 cut(s) 50
BisI GCNGC 2 cut(s) 33, 258
BlsI GCNGC 2 cut(s) 34, 259
Bme1390I CCNGG 3 cut(s) 18, 23, 108
Bme18I GGWCC 1 cut(s) 92
BmgT120I GGNCC 1 cut(s) 92
BmrFI CCNGG 3 cut(s) 18, 23, 108
BpmI CTGGAG 1 cut(s) 62
BpuEI CTTGAG 1 cut(s) 193
BsaHI GRCGYC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse1I ACTGG 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 450
BseBI CCWGG 3 cut(s) 18, 23, 108
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMI GCAATG 1 cut(s) 450
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 1 cut(s) 45
BseSI GKGCMC 1 cut(s) 315
BseXI GCAGC 2 cut(s) 19, 244
BshFI GGCC 1 cut(s) 21
BsiHKAI GWGCWC 2 cut(s) 155, 315
BslI CCNNNNNNNGG 1 cut(s) 17
BsmAI GTCTC 1 cut(s) 273
BsnI GGCC 1 cut(s) 21
Bsp1286I GDGCHC 2 cut(s) 155, 315
Bsp1407I TGTACA 1 cut(s) 387
BspACI CCGC 2 cut(s) 30, 70
BspANI GGCC 1 cut(s) 21
BspCNI CTCAG 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 450
BsrGI TGTACA 1 cut(s) 387
BsrI ACTGG 1 cut(s) 45
BssNI GRCGYC 1 cut(s) 26
Bst2UI CCWGG 3 cut(s) 18, 23, 108
Bst4CI ACNGT 2 cut(s) 88, 104
BstACI GRCGYC 1 cut(s) 26
BstAUI TGTACA 1 cut(s) 387
BstC8I GCNNGC 1 cut(s) 413
BstDEI CTNAG 1 cut(s) 149
BstMAI GTCTC 1 cut(s) 273
BstMWI GCNNNNNNNGC 1 cut(s) 32
BstNI CCWGG 3 cut(s) 18, 23, 108
BstSCI CCNGG 3 cut(s) 16, 21, 106
BstSLI GKGCMC 1 cut(s) 315
BstV1I GCAGC 2 cut(s) 19, 244
BsuRI GGCC 1 cut(s) 21
BtsIMutI CAGTG 2 cut(s) 38, 84
Cac8I GCNNGC 1 cut(s) 413
Cfr13I GGNCC 1 cut(s) 92
CseI GACGC 2 cut(s) 15, 103
Csp6I GTAC 1 cut(s) 388
CviQI GTAC 1 cut(s) 388
DdeI CTNAG 1 cut(s) 149
Ecl136II GAGCTC 1 cut(s) 153
Eco24I GRGCYC 1 cut(s) 155
Eco32I GATATC 1 cut(s) 242
Eco47I GGWCC 1 cut(s) 92
Eco53kI GAGCTC 1 cut(s) 153
Eco57I CTGAAG 1 cut(s) 159
EcoICRI GAGCTC 1 cut(s) 153
EcoRII CCWGG 3 cut(s) 16, 21, 106
EcoRV GATATC 1 cut(s) 242
EcoT38I GRGCYC 1 cut(s) 155
FblI GTMKAC 2 cut(s) 99, 117
Fnu4HI GCNGC 2 cut(s) 33, 258
FriOI GRGCYC 1 cut(s) 155
Fsp4HI GCNGC 2 cut(s) 33, 258
FspBI CTAG 1 cut(s) 50
GluI GCNGC 2 cut(s) 33, 258
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 1 cut(s) 21
HgaI GACGC 2 cut(s) 15, 103
Hin1I GRCGYC 1 cut(s) 26
HinfI GANTC 1 cut(s) 287
Hpy166II GTNNAC 4 cut(s) 100, 118, 276, 313
Hpy188I TCNGA 2 cut(s) 150, 331
Hpy8I GTNNAC 4 cut(s) 100, 118, 276, 313
HpyCH4III ACNGT 2 cut(s) 88, 104
HpyCH4V TGCA 3 cut(s) 313, 415, 443
HpyF10VI GCNNNNNNNGC 1 cut(s) 32
HpyF3I CTNAG 1 cut(s) 149
Hsp92I GRCGYC 1 cut(s) 26
LmnI GCTCC 1 cut(s) 408
Lsp1109I GCAGC 2 cut(s) 19, 244
MaeI CTAG 1 cut(s) 50
MhlI GDGCHC 2 cut(s) 155, 315
MluCI AATT 3 cut(s) 53, 78, 367
MmeI TCCRAC 2 cut(s) 70, 333
MnlI CCTC 4 cut(s) 132, 225, 301, 392
MseI TTAA 1 cut(s) 374
MspA1I CMGCKG 1 cut(s) 32
MspR9I CCNGG 3 cut(s) 18, 23, 108
MvaI CCWGG 3 cut(s) 18, 23, 108
MwoI GCNNNNNNNGC 1 cut(s) 32
PfeI GAWTC 1 cut(s) 287
PkrI GCNGC 2 cut(s) 34, 259
Psp124BI GAGCTC 1 cut(s) 155
Psp6I CCWGG 3 cut(s) 16, 21, 106
PspGI CCWGG 3 cut(s) 16, 21, 106
PspPI GGNCC 1 cut(s) 92
PsrI GAACNNNNNNTAC 2 cut(s) 413, 445
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
SacI GAGCTC 1 cut(s) 155
SaqAI TTAA 1 cut(s) 374
SatI GCNGC 2 cut(s) 33, 258
Sau96I GGNCC 1 cut(s) 92
ScrFI CCNGG 3 cut(s) 18, 23, 108
SduI GDGCHC 2 cut(s) 155, 315
SetI ASST 7 cut(s) 51, 97, 155, 173, 259, 312, 413
SinI GGWCC 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 172
SmoI CTYRAG 1 cut(s) 172
Sse9I AATT 3 cut(s) 53, 78, 367
SsiI CCGC 2 cut(s) 30, 70
SspMI CTAG 1 cut(s) 50
SstI GAGCTC 1 cut(s) 155
StyD4I CCNGG 3 cut(s) 16, 21, 106
TaaI ACNGT 2 cut(s) 88, 104
TasI AATT 3 cut(s) 53, 78, 367
TatI WGTACW 1 cut(s) 387
TfiI GAWTC 1 cut(s) 287
Tru1I TTAA 1 cut(s) 374
Tru9I TTAA 1 cut(s) 374
TscAI CASTG 2 cut(s) 45, 91
TseI GCWGC 2 cut(s) 32, 257
TspDTI ATGAA 2 cut(s) 267, 311
TspGWI ACGGA 1 cut(s) 280
TspRI CASTG 2 cut(s) 45, 91
VneI GTGCAC 1 cut(s) 311
VpaK11BI GGWCC 1 cut(s) 92
XmiI GTMKAC 2 cut(s) 99, 117
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.