Rroxscaffold_4G00285380

Appr-1'-p processing enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004383
Physical Location & Seq
Reverse (-)
6704051 .. 6706081
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00285380.1

Sequence Viewer

Length: 729 bp
ATGGCGAGCTTGCTGTGTAAAGCATCAAGATTTCTGAGCCCAGCTCCGGCGGTCAATTTAAGCCGTGGAAAAGTTGGGAGCTTTGTTAGGGTTAGAGCTAGGGTTTCGACGATGGCGAGTGGAGAAGGAGCAGTTCGGTTTCCTTTGTCTGATTCAAGCACTCTCGTGATACAAATGGGCGATATCACCAAGTGGTTCGTCGACGGCTCCACTGACGCCATTGTTAATCCAGCGAATGAGCGCATGCTTGGAGGTGGTGGTGCAGATGGAGCTATACATAGGGCTGCTGGCCCGGATCTTCTACAGGCATGCTATGGTGTCCCCGAAGCCAGGCCAGGAGTCCGCTGCCCCACTGGAGAAGCGAGAATTACCCCAGGGTTTAAGTTGCCTGCGTCTCGTGTAATTCACACTGTTGGACCTGTCTACCATGCTGGTAACAACCCTGAAGTCTATCTGAGCTCTGCATACAGGAATAGCTTGAGCATAGCCAAAGCGCACAACATTCAGTATGTTGCATTTCCTGCCATATCTTGTGGTGTGTATGGATATCCTTATGATGAAGCTGCTGAAGTGTCCCTATCCACAATTAAAGAATCCGTGAATGACATAAAGGAGGTATGGCAGCTTGTATTTGCTTCTGGACATGGCAGTAATGTGCACTTTGTTCTTTTCTCGGAAGATATTTACAATGTCTGGTTGGACAAGGCAAAGAAATTGCTTATAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Macro

Protein Analysis

242

Amino Acids

25.84

Weight (kDa)

8.65

Isoelectric Point (pI)

31.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Macro PF01661 75 - 191 2.4e-40 Macro domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 201, 423
AciI CCGC 2 cut(s) 50, 343
AclWI GGATC 1 cut(s) 303
AcuI CTGAAG 2 cut(s) 465, 588
AcyI GRCGYC 1 cut(s) 216
AdeI CACNNNGTG 1 cut(s) 192
AfiI CCNNNNNNNGG 2 cut(s) 46, 330
AgsI TTSAA 1 cut(s) 156
AjnI CCWGG 3 cut(s) 329, 334, 373
AleI CACNNNNGTG 1 cut(s) 164
AluBI AGCT 9 cut(s) 9, 44, 81, 98, 272, 459, 477, 563, 625
AluI AGCT 9 cut(s) 9, 44, 81, 98, 272, 459, 477, 563, 625
Alw21I GWGCWC 2 cut(s) 461, 660
Alw26I GTCTC 1 cut(s) 399
Alw44I GTGCAC 1 cut(s) 656
AlwI GGATC 1 cut(s) 303
AoxI GGCC 2 cut(s) 289, 332
ApaLI GTGCAC 1 cut(s) 656
ApeKI GCWGC 4 cut(s) 284, 345, 563, 622
AspLEI GCGC 2 cut(s) 243, 496
AspS9I GGNCC 2 cut(s) 290, 416
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 178
AvaII GGWCC 1 cut(s) 416
BaeGI GKGCMC 1 cut(s) 660
BanII GRGCYC 2 cut(s) 41, 461
BauI CACGAG 2 cut(s) 164, 396
Bbv12I GWGCWC 2 cut(s) 461, 660
BbvI GCAGC 4 cut(s) 271, 332, 550, 634
BccI CCATC 2 cut(s) 106, 260
BceAI ACGGC 2 cut(s) 48, 220
BciT130I CCWGG 3 cut(s) 331, 336, 375
BcnI CCSGG 1 cut(s) 293
BcoDI GTCTC 1 cut(s) 399
BfaI CTAG 1 cut(s) 99
BfmI CTRYAG 1 cut(s) 302
BisI GCNGC 4 cut(s) 285, 346, 564, 623
BlsI GCNGC 4 cut(s) 286, 347, 565, 624
Bme1390I CCNGG 4 cut(s) 293, 331, 336, 375
Bme18I GGWCC 1 cut(s) 416
BmgT120I GGNCC 2 cut(s) 290, 416
BmiI GGNNCC 1 cut(s) 208
BmrFI CCNGG 4 cut(s) 293, 331, 336, 375
BmsI GCATC 1 cut(s) 32
BplI GAGNNNNNCTC 2 cut(s) 28, 60
BpmI CTGGAG 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 499
BpuMI CCSGG 1 cut(s) 293
BsaHI GRCGYC 1 cut(s) 216
BsaJI CCNNGG 3 cut(s) 64, 373, 374
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 330
Bse1I ACTGG 1 cut(s) 358
BseBI CCWGG 3 cut(s) 331, 336, 375
BseDI CCNNGG 3 cut(s) 64, 373, 374
BseLI CCNNNNNNNGG 2 cut(s) 46, 330
BseMII CTCAG 2 cut(s) 26, 446
BseNI ACTGG 1 cut(s) 358
BseSI GKGCMC 1 cut(s) 660
BseXI GCAGC 4 cut(s) 271, 332, 550, 634
BseYI CCCAGC 1 cut(s) 40
BsgI GTGCAG 1 cut(s) 282
BshFI GGCC 2 cut(s) 291, 334
BsiHKAI GWGCWC 2 cut(s) 461, 660
BsiSI CCGG 2 cut(s) 47, 293
BslFI GGGAC 2 cut(s) 305, 559
BslI CCNNNNNNNGG 2 cut(s) 46, 330
BsmAI GTCTC 1 cut(s) 399
BsmBI CGTCTC 1 cut(s) 399
BsmFI GGGAC 2 cut(s) 305, 559
BsnI GGCC 2 cut(s) 291, 334
Bsp1286I GDGCHC 3 cut(s) 41, 461, 660
Bsp143I GATC 1 cut(s) 295
BspACI CCGC 2 cut(s) 50, 343
BspANI GGCC 2 cut(s) 291, 334
BspCNI CTCAG 2 cut(s) 27, 447
BspLI GGNNCC 1 cut(s) 208
BspPI GGATC 1 cut(s) 303
BsrI ACTGG 1 cut(s) 358
BssECI CCNNGG 3 cut(s) 64, 373, 374
BssMI GATC 1 cut(s) 295
BssNI GRCGYC 1 cut(s) 216
BssSI CACGAG 2 cut(s) 164, 396
Bst2BI CACGAG 2 cut(s) 164, 396
Bst2UI CCWGG 3 cut(s) 331, 336, 375
Bst4CI ACNGT 1 cut(s) 412
BstACI GRCGYC 1 cut(s) 216
BstAPI GCANNNNNTGC 1 cut(s) 521
BstC8I GCNNGC 6 cut(s) 7, 11, 245, 289, 310, 390
BstDEI CTNAG 2 cut(s) 35, 455
BstDSI CCRYGG 1 cut(s) 64
BstHHI GCGC 2 cut(s) 243, 496
BstKTI GATC 1 cut(s) 298
BstMAI GTCTC 1 cut(s) 399
BstMBI GATC 1 cut(s) 295
BstMWI GCNNNNNNNGC 2 cut(s) 269, 521
BstNI CCWGG 3 cut(s) 331, 336, 375
BstNSI RCATGY 2 cut(s) 247, 312
BstSCI CCNGG 4 cut(s) 291, 329, 334, 373
BstSFI CTRYAG 1 cut(s) 302
BstSLI GKGCMC 1 cut(s) 660
BstV1I GCAGC 4 cut(s) 271, 332, 550, 634
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BsuRI GGCC 2 cut(s) 291, 334
BtgI CCRYGG 1 cut(s) 64
BtsIMutI CAGTG 3 cut(s) 210, 351, 408
Cac8I GCNNGC 6 cut(s) 7, 11, 245, 289, 310, 390
CfoI GCGC 2 cut(s) 243, 496
Cfr13I GGNCC 2 cut(s) 290, 416
CseI GACGC 2 cut(s) 224, 381
CviAII CATG 4 cut(s) 244, 309, 428, 644
DdeI CTNAG 2 cut(s) 35, 455
DpnI GATC 1 cut(s) 297
DpnII GATC 1 cut(s) 295
DraIII CACNNNGTG 1 cut(s) 192
Ecl136II GAGCTC 1 cut(s) 459
Eco24I GRGCYC 2 cut(s) 41, 461
Eco32I GATATC 2 cut(s) 184, 548
Eco47I GGWCC 1 cut(s) 416
Eco53kI GAGCTC 1 cut(s) 459
Eco57I CTGAAG 2 cut(s) 465, 588
EcoICRI GAGCTC 1 cut(s) 459
EcoRII CCWGG 3 cut(s) 329, 334, 373
EcoRV GATATC 2 cut(s) 184, 548
EcoT38I GRGCYC 2 cut(s) 41, 461
Esp3I CGTCTC 1 cut(s) 399
FaeI CATG 4 cut(s) 247, 312, 431, 647
FaqI GGGAC 2 cut(s) 305, 559
FatI CATG 4 cut(s) 243, 308, 427, 643
FblI GTMKAC 2 cut(s) 201, 423
Fnu4HI GCNGC 4 cut(s) 285, 346, 564, 623
FriOI GRGCYC 2 cut(s) 41, 461
Fsp4HI GCNGC 4 cut(s) 285, 346, 564, 623
FspBI CTAG 1 cut(s) 99
GlaI GCGC 2 cut(s) 242, 495
GluI GCNGC 4 cut(s) 285, 346, 564, 623
GsaI CCCAGC 1 cut(s) 44
GsuI CTGGAG 1 cut(s) 375
HaeIII GGCC 2 cut(s) 291, 334
HapII CCGG 2 cut(s) 47, 293
HgaI GACGC 2 cut(s) 224, 381
HhaI GCGC 2 cut(s) 243, 496
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 4 cut(s) 247, 312, 431, 647
Hin6I GCGC 2 cut(s) 241, 494
HinP1I GCGC 2 cut(s) 241, 494
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 3 cut(s) 152, 339, 593
HpaII CCGG 2 cut(s) 47, 293
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 3 cut(s) 202, 424, 658
Hpy188I TCNGA 4 cut(s) 36, 151, 456, 676
Hpy188III TCNNGA 3 cut(s) 27, 166, 639
Hpy8I GTNNAC 3 cut(s) 202, 424, 658
Hpy99I CGWCG 3 cut(s) 112, 203, 206
HpyAV CCTTC 1 cut(s) 119
HpyCH4III ACNGT 1 cut(s) 412
HpyCH4V TGCA 4 cut(s) 263, 464, 515, 658
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 521
HpyF3I CTNAG 2 cut(s) 35, 455
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 4 cut(s) 247, 312, 431, 647
HspAI GCGC 2 cut(s) 241, 494
Kzo9I GATC 1 cut(s) 295
LmnI GCTCC 5 cut(s) 49, 78, 128, 212, 269
Lsp1109I GCAGC 4 cut(s) 271, 332, 550, 634
LweI GCATC 1 cut(s) 32
MaeI CTAG 1 cut(s) 99
MaeIII GTNAC 1 cut(s) 434
MalI GATC 1 cut(s) 297
MboI GATC 1 cut(s) 295
MboII GAAGA 2 cut(s) 290, 689
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 3 cut(s) 41, 461, 660
MluCI AATT 5 cut(s) 55, 366, 402, 585, 713
MlyI GAGTC 1 cut(s) 348
MmeI TCCRAC 2 cut(s) 394, 678
MnlI CCTC 2 cut(s) 245, 607
MseI TTAA 4 cut(s) 59, 225, 381, 588
MslI CAYNNNNRTG 1 cut(s) 164
MspA1I CMGCKG 1 cut(s) 345
MspI CCGG 2 cut(s) 47, 293
MspR9I CCNGG 4 cut(s) 293, 331, 336, 375
MvaI CCWGG 3 cut(s) 331, 336, 375
MwoI GCNNNNNNNGC 2 cut(s) 269, 521
NciI CCSGG 1 cut(s) 293
NdeII GATC 1 cut(s) 295
NlaIII CATG 4 cut(s) 247, 312, 431, 647
NlaIV GGNNCC 1 cut(s) 208
NspI RCATGY 2 cut(s) 247, 312
OliI CACNNNNGTG 1 cut(s) 164
PaeI GCATGC 2 cut(s) 247, 312
PasI CCCWGGG 1 cut(s) 374
PcsI WCGNNNNNNNCGW 1 cut(s) 113
PfeI GAWTC 2 cut(s) 152, 593
PkrI GCNGC 4 cut(s) 286, 347, 565, 624
PleI GAGTC 1 cut(s) 347
PpsI GAGTC 1 cut(s) 347
Psp124BI GAGCTC 1 cut(s) 461
Psp6I CCWGG 3 cut(s) 329, 334, 373
PspFI CCCAGC 1 cut(s) 40
PspGI CCWGG 3 cut(s) 329, 334, 373
PspN4I GGNNCC 1 cut(s) 208
PspPI GGNCC 2 cut(s) 290, 416
PsuI RGATCY 1 cut(s) 295
RseI CAYNNNNRTG 1 cut(s) 164
SacI GAGCTC 1 cut(s) 461
SalI GTCGAC 1 cut(s) 200
SaqAI TTAA 4 cut(s) 59, 225, 381, 588
SatI GCNGC 4 cut(s) 285, 346, 564, 623
Sau3AI GATC 1 cut(s) 295
Sau96I GGNCC 2 cut(s) 290, 416
SchI GAGTC 1 cut(s) 348
ScrFI CCNGG 4 cut(s) 293, 331, 336, 375
SduI GDGCHC 3 cut(s) 41, 461, 660
SfaNI GCATC 1 cut(s) 32
SfcI CTRYAG 1 cut(s) 302
SgrDI CGTCGACG 1 cut(s) 200
SinI GGWCC 1 cut(s) 416
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 478
SmoI CTYRAG 1 cut(s) 478
SphI GCATGC 2 cut(s) 247, 312
Sse9I AATT 5 cut(s) 55, 366, 402, 585, 713
SsiI CCGC 2 cut(s) 50, 343
SspMI CTAG 1 cut(s) 99
SstI GAGCTC 1 cut(s) 461
StyD4I CCNGG 4 cut(s) 291, 329, 334, 373
TaaI ACNGT 1 cut(s) 412
TaqI TCGA 2 cut(s) 107, 201
TasI AATT 5 cut(s) 55, 366, 402, 585, 713
TfiI GAWTC 2 cut(s) 152, 593
Tru1I TTAA 4 cut(s) 59, 225, 381, 588
Tru9I TTAA 4 cut(s) 59, 225, 381, 588
TscAI CASTG 3 cut(s) 217, 358, 415
TseI GCWGC 4 cut(s) 284, 345, 563, 622
TspDTI ATGAA 1 cut(s) 573
TspGWI ACGGA 1 cut(s) 586
TspRI CASTG 3 cut(s) 217, 358, 415
VneI GTGCAC 1 cut(s) 656
VpaK11BI GGWCC 1 cut(s) 416
XceI RCATGY 2 cut(s) 247, 312
XmiI GTMKAC 2 cut(s) 201, 423
XspI CTAG 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.