Rh1BG340200

Appr-1'-p processing enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0004383
Physical Location & Seq
Forward (+)
47438250 .. 47439518
1269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG340200.1

Sequence Viewer

Length: 465 bp
ATGCTTGGAGGTGGTGGTGGAGATGGCGCTATACATAGGGCTGCTGGCCCGGATCTTCTACAGGCATGCTATGGTGTCCCCGAAGCCAGGCCAGGAGTCCGCTGCCCCACTGGAGAAGCTAGAATTACCCCAGGGTTTAAGTTGCCTGCGTCTCATGTAATTCACACTGTTGGACCTGTCTACCGTCCCGGTAGCATCTATCCTCCTGCTAACAACCCTGAAGTTTATCTGAGCTCTGCATATAGGAATAGCTTGATCATAGCCAAAGCACACAACATTCAGTATGTTGCGTTTCCTCCCATATCTTGTGGTTCGTATGGATATCCTTATGATGAATCTGCCGAAGTGTCTCTATCCACAATCAAGGAATCCGTGAATGACATAAAGGAGGTACACTTTGTTCATAAATCGGAAGTTATTTACAATGTCTGGTTGGAAAAGGCAAAGAAATTGCTTAAAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Macro

Protein Analysis

154

Amino Acids

16.59

Weight (kDa)

8.4

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Macro PF01661 1 - 116 1.5e-32 Macro domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 180
AciI CCGC 1 cut(s) 100
AclWI GGATC 1 cut(s) 60
AcuI CTGAAG 1 cut(s) 240
AfaI GTAC 1 cut(s) 393
AfiI CCNNNNNNNGG 1 cut(s) 87
AjnI CCWGG 3 cut(s) 86, 91, 130
AluBI AGCT 3 cut(s) 119, 234, 252
AluI AGCT 3 cut(s) 119, 234, 252
Alw21I GWGCWC 1 cut(s) 236
Alw26I GTCTC 2 cut(s) 156, 354
AlwI GGATC 1 cut(s) 60
AoxI GGCC 2 cut(s) 46, 89
ApeKI GCWGC 2 cut(s) 41, 102
AspLEI GCGC 1 cut(s) 29
AspS9I GGNCC 2 cut(s) 47, 173
AsuC2I CCSGG 2 cut(s) 50, 189
AvaII GGWCC 1 cut(s) 173
BanII GRGCYC 1 cut(s) 236
Bbv12I GWGCWC 1 cut(s) 236
BbvI GCAGC 2 cut(s) 28, 89
BccI CCATC 1 cut(s) 17
BciT130I CCWGG 3 cut(s) 88, 93, 132
BclI TGATCA 1 cut(s) 255
BcnI CCSGG 2 cut(s) 50, 189
BcoDI GTCTC 2 cut(s) 156, 354
BfaI CTAG 1 cut(s) 120
BfmI CTRYAG 1 cut(s) 59
BfoI RGCGCY 1 cut(s) 30
BisI GCNGC 2 cut(s) 42, 103
BlsI GCNGC 2 cut(s) 43, 104
Bme1390I CCNGG 5 cut(s) 50, 88, 93, 132, 189
Bme18I GGWCC 1 cut(s) 173
BmgT120I GGNCC 2 cut(s) 47, 173
BmrFI CCNGG 5 cut(s) 50, 88, 93, 132, 189
BmsI GCATC 1 cut(s) 204
BpmI CTGGAG 1 cut(s) 132
BpuMI CCSGG 2 cut(s) 50, 189
BsaJI CCNNGG 2 cut(s) 130, 131
Bsc4I CCNNNNNNNGG 1 cut(s) 87
Bse1I ACTGG 1 cut(s) 115
BseBI CCWGG 3 cut(s) 88, 93, 132
BseDI CCNNGG 2 cut(s) 130, 131
BseLI CCNNNNNNNGG 1 cut(s) 87
BseMII CTCAG 1 cut(s) 221
BseNI ACTGG 1 cut(s) 115
BseXI GCAGC 2 cut(s) 28, 89
BshFI GGCC 2 cut(s) 48, 91
BsiHKAI GWGCWC 1 cut(s) 236
BsiSI CCGG 2 cut(s) 50, 189
BslFI GGGAC 2 cut(s) 62, 171
BslI CCNNNNNNNGG 1 cut(s) 87
BsmAI GTCTC 2 cut(s) 156, 354
BsmBI CGTCTC 1 cut(s) 156
BsmFI GGGAC 2 cut(s) 62, 171
BsnI GGCC 2 cut(s) 48, 91
Bsp1286I GDGCHC 1 cut(s) 236
Bsp143I GATC 2 cut(s) 52, 255
BspACI CCGC 1 cut(s) 100
BspANI GGCC 2 cut(s) 48, 91
BspCNI CTCAG 1 cut(s) 222
BspPI GGATC 1 cut(s) 60
BsrI ACTGG 1 cut(s) 115
BssECI CCNNGG 2 cut(s) 130, 131
BssMI GATC 2 cut(s) 52, 255
Bst2UI CCWGG 3 cut(s) 88, 93, 132
Bst4CI ACNGT 2 cut(s) 169, 185
BstC8I GCNNGC 3 cut(s) 46, 67, 147
BstDEI CTNAG 1 cut(s) 230
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 1 cut(s) 29
BstKTI GATC 2 cut(s) 55, 258
BstMAI GTCTC 2 cut(s) 156, 354
BstMBI GATC 2 cut(s) 52, 255
BstNI CCWGG 3 cut(s) 88, 93, 132
BstNSI RCATGY 1 cut(s) 69
BstSCI CCNGG 5 cut(s) 48, 86, 91, 130, 187
BstSFI CTRYAG 1 cut(s) 59
BstV1I GCAGC 2 cut(s) 28, 89
BstX2I RGATCY 1 cut(s) 52
BstYI RGATCY 1 cut(s) 52
BsuRI GGCC 2 cut(s) 48, 91
BtsIMutI CAGTG 2 cut(s) 108, 165
Cac8I GCNNGC 3 cut(s) 46, 67, 147
CfoI GCGC 1 cut(s) 29
Cfr13I GGNCC 2 cut(s) 47, 173
CseI GACGC 1 cut(s) 138
Csp6I GTAC 1 cut(s) 392
CviAII CATG 2 cut(s) 66, 155
CviJI RGCY 9 cut(s) 41, 48, 86, 91, 119, 234, 252, 263, 461
CviKI_1 RGCY 9 cut(s) 41, 48, 86, 91, 119, 234, 252, 263, 461
CviQI GTAC 1 cut(s) 392
DdeI CTNAG 1 cut(s) 230
DpnI GATC 2 cut(s) 54, 257
DpnII GATC 2 cut(s) 52, 255
Ecl136II GAGCTC 1 cut(s) 234
Eco24I GRGCYC 1 cut(s) 236
Eco32I GATATC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 173
Eco53kI GAGCTC 1 cut(s) 234
Eco57I CTGAAG 1 cut(s) 240
EcoICRI GAGCTC 1 cut(s) 234
EcoRII CCWGG 3 cut(s) 86, 91, 130
EcoRV GATATC 1 cut(s) 323
EcoT38I GRGCYC 1 cut(s) 236
Esp3I CGTCTC 1 cut(s) 156
FaeI CATG 2 cut(s) 69, 158
FaqI GGGAC 2 cut(s) 62, 171
FatI CATG 2 cut(s) 65, 154
FbaI TGATCA 1 cut(s) 255
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 2 cut(s) 42, 103
FriOI GRGCYC 1 cut(s) 236
Fsp4HI GCNGC 2 cut(s) 42, 103
FspBI CTAG 1 cut(s) 120
GlaI GCGC 1 cut(s) 28
GluI GCNGC 2 cut(s) 42, 103
GsuI CTGGAG 1 cut(s) 132
HaeII RGCGCY 1 cut(s) 30
HaeIII GGCC 2 cut(s) 48, 91
HapII CCGG 2 cut(s) 50, 189
HgaI GACGC 1 cut(s) 138
HhaI GCGC 1 cut(s) 29
Hin1II CATG 2 cut(s) 69, 158
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HinfI GANTC 3 cut(s) 96, 335, 368
HpaII CCGG 2 cut(s) 50, 189
Hpy166II GTNNAC 2 cut(s) 181, 394
Hpy188I TCNGA 2 cut(s) 231, 412
Hpy8I GTNNAC 2 cut(s) 181, 394
HpyCH4III ACNGT 2 cut(s) 169, 185
HpyCH4V TGCA 1 cut(s) 239
HpyF3I CTNAG 1 cut(s) 230
Hsp92II CATG 2 cut(s) 69, 158
HspAI GCGC 1 cut(s) 27
Ksp22I TGATCA 1 cut(s) 255
Kzo9I GATC 2 cut(s) 52, 255
Lsp1109I GCAGC 2 cut(s) 28, 89
LweI GCATC 1 cut(s) 204
MaeI CTAG 1 cut(s) 120
MalI GATC 2 cut(s) 54, 257
MboI GATC 2 cut(s) 52, 255
MboII GAAGA 1 cut(s) 47
MflI RGATCY 1 cut(s) 52
MhlI GDGCHC 1 cut(s) 236
MluCI AATT 3 cut(s) 123, 159, 449
MlyI GAGTC 1 cut(s) 105
MmeI TCCRAC 2 cut(s) 151, 414
MnlI CCTC 3 cut(s) 213, 306, 382
MseI TTAA 2 cut(s) 138, 456
MspA1I CMGCKG 1 cut(s) 102
MspI CCGG 2 cut(s) 50, 189
MspR9I CCNGG 5 cut(s) 50, 88, 93, 132, 189
MvaI CCWGG 3 cut(s) 88, 93, 132
NciI CCSGG 2 cut(s) 50, 189
NdeII GATC 2 cut(s) 52, 255
NlaIII CATG 2 cut(s) 69, 158
NspI RCATGY 1 cut(s) 69
PaeI GCATGC 1 cut(s) 69
PasI CCCWGGG 1 cut(s) 131
PfeI GAWTC 2 cut(s) 335, 368
PkrI GCNGC 2 cut(s) 43, 104
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
Psp124BI GAGCTC 1 cut(s) 236
Psp6I CCWGG 3 cut(s) 86, 91, 130
PspGI CCWGG 3 cut(s) 86, 91, 130
PspPI GGNCC 2 cut(s) 47, 173
PsrI GAACNNNNNNTAC 2 cut(s) 384, 416
PsuI RGATCY 1 cut(s) 52
RsaI GTAC 1 cut(s) 393
RsaNI GTAC 1 cut(s) 392
SacI GAGCTC 1 cut(s) 236
SaqAI TTAA 2 cut(s) 138, 456
SatI GCNGC 2 cut(s) 42, 103
Sau3AI GATC 2 cut(s) 52, 255
Sau96I GGNCC 2 cut(s) 47, 173
SchI GAGTC 1 cut(s) 105
ScrFI CCNGG 5 cut(s) 50, 88, 93, 132, 189
SduI GDGCHC 1 cut(s) 236
SetI ASST 6 cut(s) 13, 121, 178, 236, 254, 393
SfaNI GCATC 1 cut(s) 204
SfcI CTRYAG 1 cut(s) 59
SinI GGWCC 1 cut(s) 173
SphI GCATGC 1 cut(s) 69
Sse9I AATT 3 cut(s) 123, 159, 449
SsiI CCGC 1 cut(s) 100
SspMI CTAG 1 cut(s) 120
SstI GAGCTC 1 cut(s) 236
StyD4I CCNGG 5 cut(s) 48, 86, 91, 130, 187
TaaI ACNGT 2 cut(s) 169, 185
TasI AATT 3 cut(s) 123, 159, 449
TfiI GAWTC 2 cut(s) 335, 368
Tru1I TTAA 2 cut(s) 138, 456
Tru9I TTAA 2 cut(s) 138, 456
TscAI CASTG 2 cut(s) 115, 172
TseI GCWGC 2 cut(s) 41, 102
TspDTI ATGAA 2 cut(s) 348, 392
TspGWI ACGGA 1 cut(s) 361
TspRI CASTG 2 cut(s) 115, 172
VpaK11BI GGWCC 1 cut(s) 173
XceI RCATGY 1 cut(s) 69
XmiI GTMKAC 1 cut(s) 180
XspI CTAG 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.