RH1DG371200

Appr-1'-p processing enzyme

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0004383
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG371200.1

Sequence Viewer

Length: 516 bp
ATGGGGGATATCACCAAGTGGTTCGTCGACGGCTCCACTGACGCCATTGTTAATTCAGCGAATGAGCGAATGCTTGGAGCTATACATAGGGCTGCTGGCCCGGATCTTCTACAGGCATGCTATGGTGTCCCCGAAGCCAGGCCAGGCGTCCGCTGCCCCACTGGAGAAGCTAGAATTACCCTAGGGTTTAAGTTGCCTGCGTCTCATGTAATTCACACTGTTGGACCTGTCTACCATCCTGGTAGCGTCTACCCTCCTGCTAACAACCCTGAAGTTTATCTGAGCTCTGCATCTAGGAATAGCTTGAGCATAGCCAAAGCACACAACATTCAGTATGTTGCGTTTCCTCCCATATCTTGTGGTTCGTATGGATATCCTTATGATGAAGCTGCCGAAGTGTCCCTAACCACAATTAAAGAATCCGTGAATGACATAAAGGAGGTGCACTTTGTTCTTTTCTCGGAAGATATTTACAATGTCTGGTTGGACAAGGCAAAGAAATTGCTTAAAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Macro

Protein Analysis

171

Amino Acids

18.57

Weight (kDa)

6.28

Isoelectric Point (pI)

32.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Macro PF01661 17 - 133 1.6e-29 Macro domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 27, 231, 249
AciI CCGC 1 cut(s) 151
AclWI GGATC 1 cut(s) 111
AcuI CTGAAG 1 cut(s) 291
AcyI GRCGYC 2 cut(s) 42, 147
AdeI CACNNNGTG 1 cut(s) 18
AfiI CCNNNNNNNGG 1 cut(s) 138
AjnI CCWGG 3 cut(s) 137, 142, 238
AluBI AGCT 5 cut(s) 80, 170, 285, 303, 389
AluI AGCT 5 cut(s) 80, 170, 285, 303, 389
Alw21I GWGCWC 2 cut(s) 287, 447
Alw26I GTCTC 1 cut(s) 207
Alw44I GTGCAC 1 cut(s) 443
AlwI GGATC 1 cut(s) 111
AoxI GGCC 2 cut(s) 97, 140
ApaLI GTGCAC 1 cut(s) 443
ApeKI GCWGC 3 cut(s) 92, 153, 389
AspA2I CCTAGG 1 cut(s) 181
AspS9I GGNCC 2 cut(s) 98, 224
AsuC2I CCSGG 1 cut(s) 101
AsuHPI GGTGA 1 cut(s) 4
AvaII GGWCC 1 cut(s) 224
AvrII CCTAGG 1 cut(s) 181
BaeGI GKGCMC 1 cut(s) 447
BanII GRGCYC 1 cut(s) 287
Bbv12I GWGCWC 2 cut(s) 287, 447
BbvI GCAGC 3 cut(s) 79, 140, 376
BccI CCATC 1 cut(s) 243
BceAI ACGGC 1 cut(s) 46
BciT130I CCWGG 3 cut(s) 139, 144, 240
BcnI CCSGG 1 cut(s) 101
BcoDI GTCTC 1 cut(s) 207
BfaI CTAG 3 cut(s) 171, 182, 294
BfmI CTRYAG 1 cut(s) 110
BisI GCNGC 3 cut(s) 93, 154, 390
BlnI CCTAGG 1 cut(s) 181
BlsI GCNGC 3 cut(s) 94, 155, 391
Bme1390I CCNGG 4 cut(s) 101, 139, 144, 240
Bme18I GGWCC 1 cut(s) 224
BmgT120I GGNCC 2 cut(s) 98, 224
BmiI GGNNCC 1 cut(s) 34
BmrFI CCNGG 4 cut(s) 101, 139, 144, 240
BmsI GCATC 1 cut(s) 299
BpmI CTGGAG 1 cut(s) 183
BpuEI CTTGAG 1 cut(s) 325
BpuMI CCSGG 1 cut(s) 101
BsaHI GRCGYC 2 cut(s) 42, 147
BsaJI CCNNGG 1 cut(s) 181
Bsc4I CCNNNNNNNGG 1 cut(s) 138
Bse1I ACTGG 1 cut(s) 166
BseBI CCWGG 3 cut(s) 139, 144, 240
BseDI CCNNGG 1 cut(s) 181
BseGI GGATG 1 cut(s) 235
BseLI CCNNNNNNNGG 1 cut(s) 138
BseMII CTCAG 1 cut(s) 272
BseNI ACTGG 1 cut(s) 166
BseSI GKGCMC 1 cut(s) 447
BseXI GCAGC 3 cut(s) 79, 140, 376
BshFI GGCC 2 cut(s) 99, 142
BsiHKAI GWGCWC 2 cut(s) 287, 447
BsiSI CCGG 1 cut(s) 101
BslFI GGGAC 2 cut(s) 113, 385
BslI CCNNNNNNNGG 1 cut(s) 138
BsmAI GTCTC 1 cut(s) 207
BsmBI CGTCTC 1 cut(s) 207
BsmFI GGGAC 2 cut(s) 113, 385
BsmI GAATGC 1 cut(s) 75
BsnI GGCC 2 cut(s) 99, 142
Bsp1286I GDGCHC 2 cut(s) 287, 447
Bsp143I GATC 1 cut(s) 103
BspACI CCGC 1 cut(s) 151
BspANI GGCC 2 cut(s) 99, 142
BspCNI CTCAG 1 cut(s) 273
BspLI GGNNCC 1 cut(s) 34
BspPI GGATC 1 cut(s) 111
BsrI ACTGG 1 cut(s) 166
BssECI CCNNGG 1 cut(s) 181
BssMI GATC 1 cut(s) 103
BssNI GRCGYC 2 cut(s) 42, 147
BssT1I CCWWGG 1 cut(s) 181
Bst2UI CCWGG 3 cut(s) 139, 144, 240
Bst4CI ACNGT 1 cut(s) 220
BstACI GRCGYC 2 cut(s) 42, 147
BstC8I GCNNGC 3 cut(s) 97, 118, 198
BstDEI CTNAG 1 cut(s) 281
BstF5I GGATG 1 cut(s) 235
BstKTI GATC 1 cut(s) 106
BstMAI GTCTC 1 cut(s) 207
BstMBI GATC 1 cut(s) 103
BstMWI GCNNNNNNNGC 1 cut(s) 153
BstNI CCWGG 3 cut(s) 139, 144, 240
BstNSI RCATGY 1 cut(s) 120
BstSCI CCNGG 4 cut(s) 99, 137, 142, 238
BstSFI CTRYAG 1 cut(s) 110
BstSLI GKGCMC 1 cut(s) 447
BstV1I GCAGC 3 cut(s) 79, 140, 376
BstX2I RGATCY 1 cut(s) 103
BstYI RGATCY 1 cut(s) 103
BsuRI GGCC 2 cut(s) 99, 142
BtsCI GGATG 1 cut(s) 235
BtsIMutI CAGTG 3 cut(s) 36, 159, 216
Cac8I GCNNGC 3 cut(s) 97, 118, 198
Cfr13I GGNCC 2 cut(s) 98, 224
CseI GACGC 4 cut(s) 50, 136, 189, 235
CviAII CATG 2 cut(s) 117, 206
DdeI CTNAG 1 cut(s) 281
DpnI GATC 1 cut(s) 105
DpnII GATC 1 cut(s) 103
DraIII CACNNNGTG 1 cut(s) 18
Ecl136II GAGCTC 1 cut(s) 285
Eco130I CCWWGG 1 cut(s) 181
Eco24I GRGCYC 1 cut(s) 287
Eco32I GATATC 2 cut(s) 10, 374
Eco47I GGWCC 1 cut(s) 224
Eco53kI GAGCTC 1 cut(s) 285
Eco57I CTGAAG 1 cut(s) 291
EcoICRI GAGCTC 1 cut(s) 285
EcoRII CCWGG 3 cut(s) 137, 142, 238
EcoRV GATATC 2 cut(s) 10, 374
EcoT14I CCWWGG 1 cut(s) 181
EcoT38I GRGCYC 1 cut(s) 287
ErhI CCWWGG 1 cut(s) 181
Esp3I CGTCTC 1 cut(s) 207
FaeI CATG 2 cut(s) 120, 209
FaqI GGGAC 2 cut(s) 113, 385
FatI CATG 2 cut(s) 116, 205
FblI GTMKAC 3 cut(s) 27, 231, 249
Fnu4HI GCNGC 3 cut(s) 93, 154, 390
FokI GGATG 1 cut(s) 222
FriOI GRGCYC 1 cut(s) 287
Fsp4HI GCNGC 3 cut(s) 93, 154, 390
FspBI CTAG 3 cut(s) 171, 182, 294
GluI GCNGC 3 cut(s) 93, 154, 390
GsuI CTGGAG 1 cut(s) 183
HaeIII GGCC 2 cut(s) 99, 142
HapII CCGG 1 cut(s) 101
HgaI GACGC 4 cut(s) 50, 136, 189, 235
Hin1I GRCGYC 2 cut(s) 42, 147
Hin1II CATG 2 cut(s) 120, 209
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HinfI GANTC 1 cut(s) 419
HpaII CCGG 1 cut(s) 101
HphI GGTGA 1 cut(s) 4
Hpy166II GTNNAC 4 cut(s) 28, 232, 250, 445
Hpy188I TCNGA 2 cut(s) 282, 463
Hpy8I GTNNAC 4 cut(s) 28, 232, 250, 445
Hpy99I CGWCG 2 cut(s) 29, 32
HpyCH4III ACNGT 1 cut(s) 220
HpyCH4V TGCA 2 cut(s) 290, 445
HpyF10VI GCNNNNNNNGC 1 cut(s) 153
HpyF3I CTNAG 1 cut(s) 281
Hsp92I GRCGYC 2 cut(s) 42, 147
Hsp92II CATG 2 cut(s) 120, 209
Kzo9I GATC 1 cut(s) 103
LmnI GCTCC 2 cut(s) 38, 77
Lsp1109I GCAGC 3 cut(s) 79, 140, 376
LweI GCATC 1 cut(s) 299
MaeI CTAG 3 cut(s) 171, 182, 294
MalI GATC 1 cut(s) 105
MboI GATC 1 cut(s) 103
MboII GAAGA 2 cut(s) 98, 476
MflI RGATCY 1 cut(s) 103
MhlI GDGCHC 2 cut(s) 287, 447
MluCI AATT 5 cut(s) 52, 174, 210, 411, 500
MmeI TCCRAC 2 cut(s) 202, 465
MnlI CCTC 3 cut(s) 264, 357, 433
MseI TTAA 4 cut(s) 51, 189, 414, 507
MspA1I CMGCKG 1 cut(s) 153
MspI CCGG 1 cut(s) 101
MspR9I CCNGG 4 cut(s) 101, 139, 144, 240
Mva1269I GAATGC 1 cut(s) 75
MvaI CCWGG 3 cut(s) 139, 144, 240
MwoI GCNNNNNNNGC 1 cut(s) 153
NciI CCSGG 1 cut(s) 101
NdeII GATC 1 cut(s) 103
NlaIII CATG 2 cut(s) 120, 209
NlaIV GGNNCC 1 cut(s) 34
NspI RCATGY 1 cut(s) 120
PaeI GCATGC 1 cut(s) 120
PctI GAATGC 1 cut(s) 75
PfeI GAWTC 1 cut(s) 419
PkrI GCNGC 3 cut(s) 94, 155, 391
Psp124BI GAGCTC 1 cut(s) 287
Psp6I CCWGG 3 cut(s) 137, 142, 238
PspGI CCWGG 3 cut(s) 137, 142, 238
PspN4I GGNNCC 1 cut(s) 34
PspPI GGNCC 2 cut(s) 98, 224
PsuI RGATCY 1 cut(s) 103
SacI GAGCTC 1 cut(s) 287
SalI GTCGAC 1 cut(s) 26
SaqAI TTAA 4 cut(s) 51, 189, 414, 507
SatI GCNGC 3 cut(s) 93, 154, 390
Sau3AI GATC 1 cut(s) 103
Sau96I GGNCC 2 cut(s) 98, 224
ScrFI CCNGG 4 cut(s) 101, 139, 144, 240
SduI GDGCHC 2 cut(s) 287, 447
SetI ASST 7 cut(s) 82, 172, 229, 287, 305, 391, 444
SfaNI GCATC 1 cut(s) 299
SfcI CTRYAG 1 cut(s) 110
SgrDI CGTCGACG 1 cut(s) 26
SinI GGWCC 1 cut(s) 224
SmlI CTYRAG 1 cut(s) 304
SmoI CTYRAG 1 cut(s) 304
SphI GCATGC 1 cut(s) 120
Sse9I AATT 5 cut(s) 52, 174, 210, 411, 500
SsiI CCGC 1 cut(s) 151
SspMI CTAG 3 cut(s) 171, 182, 294
SstI GAGCTC 1 cut(s) 287
StyD4I CCNGG 4 cut(s) 99, 137, 142, 238
StyI CCWWGG 1 cut(s) 181
TaaI ACNGT 1 cut(s) 220
TaqI TCGA 1 cut(s) 27
TasI AATT 5 cut(s) 52, 174, 210, 411, 500
TfiI GAWTC 1 cut(s) 419
Tru1I TTAA 4 cut(s) 51, 189, 414, 507
Tru9I TTAA 4 cut(s) 51, 189, 414, 507
TscAI CASTG 3 cut(s) 43, 166, 223
TseI GCWGC 3 cut(s) 92, 153, 389
TspDTI ATGAA 1 cut(s) 399
TspGWI ACGGA 1 cut(s) 412
TspRI CASTG 3 cut(s) 43, 166, 223
VneI GTGCAC 1 cut(s) 443
VpaK11BI GGWCC 1 cut(s) 224
XceI RCATGY 1 cut(s) 120
XmaJI CCTAGG 1 cut(s) 181
XmiI GTMKAC 3 cut(s) 27, 231, 249
XspI CTAG 3 cut(s) 171, 182, 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.