Rroxscaffold_4G00285390

Appr-1'-p processing enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004383
Physical Location & Seq
Reverse (-)
6706796 .. 6708880
2085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00285390.1

Sequence Viewer

Length: 597 bp
ATGGCGAGTGGAGAAGGAGCAGTTCGGTTTCTTTTGTCTGATTCAAGCACTCTCGTGATACAAATGGGGGATATCACCAAGTGGTTCGTCGACGGCTCCACTGACGCCATTGTTAATTCAGCGAATGAGCGAATGCTTGGAGGTGGTGGTGGAAATGGCGCTATACATAGGGCTGCTGGCCCGGATCTTCTACAAGCATGCTATGGTGTCCCCGAAGCCAGGCCAGGAGTCCGCTGCCCCACTGGAGAAGCTAGAATTACCCCAGGGTTTAAGTTGCCTGCGTCTCATGTAATTCACACTGTTGGACCTGTCTACCGTCCTGGTAGCATCTACCCTCCTGCTAACAACCCTGAAGTTTATCTGAGCTCTGCATATAGGAATAGCTTGAGCATAGCCAAAGCACACAACATTCAGTATGTTGCATTTCCTGCCATATCTTGTGGTTCGTATGGATATCCTTATGATGAAGCTGCCGAAGTGTCTCTATCCACAATTAAGGATTCCGTGAATGACAGAAAGGAGGTGCACTTTGTTCATTTCTCGGAAGATACTTACAATGTCTGGTTGGAAAAGGCAAAGAAATTGCTTAAAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Macro

Protein Analysis

198

Amino Acids

21.25

Weight (kDa)

6.58

Isoelectric Point (pI)

30.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Macro PF01661 38 - 160 3.1e-37 Macro domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 90, 312
AciI CCGC 1 cut(s) 232
AclWI GGATC 1 cut(s) 192
AcuI CTGAAG 1 cut(s) 372
AcyI GRCGYC 1 cut(s) 105
AdeI CACNNNGTG 1 cut(s) 81
AfiI CCNNNNNNNGG 1 cut(s) 219
AgsI TTSAA 1 cut(s) 45
AjnI CCWGG 4 cut(s) 218, 223, 262, 319
AleI CACNNNNGTG 1 cut(s) 53
AluBI AGCT 4 cut(s) 251, 366, 384, 470
AluI AGCT 4 cut(s) 251, 366, 384, 470
Alw21I GWGCWC 2 cut(s) 368, 528
Alw26I GTCTC 2 cut(s) 288, 486
Alw44I GTGCAC 1 cut(s) 524
AlwI GGATC 1 cut(s) 192
AoxI GGCC 2 cut(s) 178, 221
ApaLI GTGCAC 1 cut(s) 524
ApeKI GCWGC 3 cut(s) 173, 234, 470
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 2 cut(s) 179, 305
AsuC2I CCSGG 1 cut(s) 182
AsuHPI GGTGA 1 cut(s) 67
AvaII GGWCC 1 cut(s) 305
BaeGI GKGCMC 1 cut(s) 528
BanII GRGCYC 1 cut(s) 368
BauI CACGAG 1 cut(s) 53
Bbv12I GWGCWC 2 cut(s) 368, 528
BbvI GCAGC 3 cut(s) 160, 221, 457
BceAI ACGGC 1 cut(s) 109
BciT130I CCWGG 4 cut(s) 220, 225, 264, 321
BcnI CCSGG 1 cut(s) 182
BcoDI GTCTC 2 cut(s) 288, 486
BfaI CTAG 1 cut(s) 252
BfoI RGCGCY 1 cut(s) 162
BisI GCNGC 3 cut(s) 174, 235, 471
BlsI GCNGC 3 cut(s) 175, 236, 472
Bme1390I CCNGG 5 cut(s) 182, 220, 225, 264, 321
Bme18I GGWCC 1 cut(s) 305
BmgT120I GGNCC 2 cut(s) 179, 305
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 5 cut(s) 182, 220, 225, 264, 321
BmsI GCATC 1 cut(s) 336
BpmI CTGGAG 1 cut(s) 264
BpuEI CTTGAG 1 cut(s) 406
BpuMI CCSGG 1 cut(s) 182
BsaHI GRCGYC 1 cut(s) 105
BsaJI CCNNGG 2 cut(s) 262, 263
Bsc4I CCNNNNNNNGG 1 cut(s) 219
Bse1I ACTGG 1 cut(s) 247
BseBI CCWGG 4 cut(s) 220, 225, 264, 321
BseDI CCNNGG 2 cut(s) 262, 263
BseLI CCNNNNNNNGG 1 cut(s) 219
BseMII CTCAG 1 cut(s) 353
BseNI ACTGG 1 cut(s) 247
BseSI GKGCMC 1 cut(s) 528
BseXI GCAGC 3 cut(s) 160, 221, 457
BshFI GGCC 2 cut(s) 180, 223
BsiHKAI GWGCWC 2 cut(s) 368, 528
BsiSI CCGG 1 cut(s) 182
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 1 cut(s) 219
BsmAI GTCTC 2 cut(s) 288, 486
BsmBI CGTCTC 1 cut(s) 288
BsmFI GGGAC 1 cut(s) 194
BsmI GAATGC 1 cut(s) 138
BsnI GGCC 2 cut(s) 180, 223
Bsp1286I GDGCHC 2 cut(s) 368, 528
Bsp143I GATC 1 cut(s) 184
BspACI CCGC 1 cut(s) 232
BspANI GGCC 2 cut(s) 180, 223
BspCNI CTCAG 1 cut(s) 354
BspLI GGNNCC 1 cut(s) 97
BspPI GGATC 1 cut(s) 192
BsrI ACTGG 1 cut(s) 247
BssECI CCNNGG 2 cut(s) 262, 263
BssMI GATC 1 cut(s) 184
BssNI GRCGYC 1 cut(s) 105
BssSI CACGAG 1 cut(s) 53
Bst2BI CACGAG 1 cut(s) 53
Bst2UI CCWGG 4 cut(s) 220, 225, 264, 321
Bst4CI ACNGT 2 cut(s) 301, 317
BstACI GRCGYC 1 cut(s) 105
BstAPI GCANNNNNTGC 1 cut(s) 428
BstC8I GCNNGC 3 cut(s) 178, 199, 279
BstDEI CTNAG 1 cut(s) 362
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 1 cut(s) 187
BstMAI GTCTC 2 cut(s) 288, 486
BstMBI GATC 1 cut(s) 184
BstMWI GCNNNNNNNGC 1 cut(s) 428
BstNI CCWGG 4 cut(s) 220, 225, 264, 321
BstNSI RCATGY 1 cut(s) 201
BstSCI CCNGG 5 cut(s) 180, 218, 223, 262, 319
BstSLI GKGCMC 1 cut(s) 528
BstV1I GCAGC 3 cut(s) 160, 221, 457
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
BsuRI GGCC 2 cut(s) 180, 223
BtsIMutI CAGTG 3 cut(s) 99, 240, 297
Cac8I GCNNGC 3 cut(s) 178, 199, 279
CfoI GCGC 1 cut(s) 161
Cfr13I GGNCC 2 cut(s) 179, 305
CseI GACGC 2 cut(s) 113, 270
CviAII CATG 2 cut(s) 198, 287
DdeI CTNAG 1 cut(s) 362
DpnI GATC 1 cut(s) 186
DpnII GATC 1 cut(s) 184
DraIII CACNNNGTG 1 cut(s) 81
Ecl136II GAGCTC 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 368
Eco32I GATATC 2 cut(s) 73, 455
Eco47I GGWCC 1 cut(s) 305
Eco53kI GAGCTC 1 cut(s) 366
Eco57I CTGAAG 1 cut(s) 372
EcoICRI GAGCTC 1 cut(s) 366
EcoRII CCWGG 4 cut(s) 218, 223, 262, 319
EcoRV GATATC 2 cut(s) 73, 455
EcoT38I GRGCYC 1 cut(s) 368
Esp3I CGTCTC 1 cut(s) 288
FaeI CATG 2 cut(s) 201, 290
FaqI GGGAC 1 cut(s) 194
FatI CATG 2 cut(s) 197, 286
FblI GTMKAC 2 cut(s) 90, 312
Fnu4HI GCNGC 3 cut(s) 174, 235, 471
FriOI GRGCYC 1 cut(s) 368
Fsp4HI GCNGC 3 cut(s) 174, 235, 471
FspBI CTAG 1 cut(s) 252
GlaI GCGC 1 cut(s) 160
GluI GCNGC 3 cut(s) 174, 235, 471
GsuI CTGGAG 1 cut(s) 264
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 2 cut(s) 180, 223
HapII CCGG 1 cut(s) 182
HgaI GACGC 2 cut(s) 113, 270
HhaI GCGC 1 cut(s) 161
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 2 cut(s) 201, 290
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HinfI GANTC 3 cut(s) 41, 228, 500
HpaII CCGG 1 cut(s) 182
HphI GGTGA 1 cut(s) 67
Hpy166II GTNNAC 3 cut(s) 91, 313, 526
Hpy188I TCNGA 3 cut(s) 40, 363, 544
Hpy188III TCNNGA 1 cut(s) 55
Hpy8I GTNNAC 3 cut(s) 91, 313, 526
Hpy99I CGWCG 2 cut(s) 92, 95
HpyAV CCTTC 1 cut(s) 8
HpyCH4III ACNGT 2 cut(s) 301, 317
HpyCH4V TGCA 3 cut(s) 371, 422, 526
HpyF10VI GCNNNNNNNGC 1 cut(s) 428
HpyF3I CTNAG 1 cut(s) 362
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 2 cut(s) 201, 290
HspAI GCGC 1 cut(s) 159
Kzo9I GATC 1 cut(s) 184
LmnI GCTCC 2 cut(s) 17, 101
Lsp1109I GCAGC 3 cut(s) 160, 221, 457
LweI GCATC 1 cut(s) 336
MaeI CTAG 1 cut(s) 252
MalI GATC 1 cut(s) 186
MboI GATC 1 cut(s) 184
MboII GAAGA 2 cut(s) 179, 557
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 2 cut(s) 368, 528
MluCI AATT 5 cut(s) 115, 255, 291, 492, 581
MlyI GAGTC 1 cut(s) 237
MmeI TCCRAC 2 cut(s) 283, 546
MnlI CCTC 3 cut(s) 134, 345, 514
MseI TTAA 4 cut(s) 114, 270, 495, 588
MslI CAYNNNNRTG 1 cut(s) 53
MspA1I CMGCKG 1 cut(s) 234
MspI CCGG 1 cut(s) 182
MspR9I CCNGG 5 cut(s) 182, 220, 225, 264, 321
Mva1269I GAATGC 1 cut(s) 138
MvaI CCWGG 4 cut(s) 220, 225, 264, 321
MwoI GCNNNNNNNGC 1 cut(s) 428
NciI CCSGG 1 cut(s) 182
NdeII GATC 1 cut(s) 184
NlaIII CATG 2 cut(s) 201, 290
NlaIV GGNNCC 1 cut(s) 97
NspI RCATGY 1 cut(s) 201
OliI CACNNNNGTG 1 cut(s) 53
PaeI GCATGC 1 cut(s) 201
PasI CCCWGGG 1 cut(s) 263
PctI GAATGC 1 cut(s) 138
PfeI GAWTC 2 cut(s) 41, 500
PkrI GCNGC 3 cut(s) 175, 236, 472
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
Psp124BI GAGCTC 1 cut(s) 368
Psp6I CCWGG 4 cut(s) 218, 223, 262, 319
PspGI CCWGG 4 cut(s) 218, 223, 262, 319
PspN4I GGNNCC 1 cut(s) 97
PspPI GGNCC 2 cut(s) 179, 305
PsuI RGATCY 1 cut(s) 184
RseI CAYNNNNRTG 1 cut(s) 53
SacI GAGCTC 1 cut(s) 368
SalI GTCGAC 1 cut(s) 89
SaqAI TTAA 4 cut(s) 114, 270, 495, 588
SatI GCNGC 3 cut(s) 174, 235, 471
Sau3AI GATC 1 cut(s) 184
Sau96I GGNCC 2 cut(s) 179, 305
SchI GAGTC 1 cut(s) 237
ScrFI CCNGG 5 cut(s) 182, 220, 225, 264, 321
SduI GDGCHC 2 cut(s) 368, 528
SetI ASST 7 cut(s) 145, 253, 310, 368, 386, 472, 525
SfaNI GCATC 1 cut(s) 336
SgrDI CGTCGACG 1 cut(s) 89
SinI GGWCC 1 cut(s) 305
SmiMI CAYNNNNRTG 1 cut(s) 53
SmlI CTYRAG 1 cut(s) 385
SmoI CTYRAG 1 cut(s) 385
SphI GCATGC 1 cut(s) 201
Sse9I AATT 5 cut(s) 115, 255, 291, 492, 581
SsiI CCGC 1 cut(s) 232
SspMI CTAG 1 cut(s) 252
SstI GAGCTC 1 cut(s) 368
StyD4I CCNGG 5 cut(s) 180, 218, 223, 262, 319
TaaI ACNGT 2 cut(s) 301, 317
TaqI TCGA 1 cut(s) 90
TasI AATT 5 cut(s) 115, 255, 291, 492, 581
TfiI GAWTC 2 cut(s) 41, 500
Tru1I TTAA 4 cut(s) 114, 270, 495, 588
Tru9I TTAA 4 cut(s) 114, 270, 495, 588
TscAI CASTG 3 cut(s) 106, 247, 304
TseI GCWGC 3 cut(s) 173, 234, 470
TspDTI ATGAA 2 cut(s) 480, 524
TspGWI ACGGA 1 cut(s) 493
TspRI CASTG 3 cut(s) 106, 247, 304
VneI GTGCAC 1 cut(s) 524
VpaK11BI GGWCC 1 cut(s) 305
XceI RCATGY 1 cut(s) 201
XmiI GTMKAC 2 cut(s) 90, 312
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.