Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0003570

Physical Location & Seq

Genomic Coordinates Forward (+)

657583 .. 661362

Gene Structure 3780 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1004100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1077 bp

ATGGCTAATAATCTCGGCCCAGCTCTGGACAAAAAATTGGTTTTGTGTGAAGATTCTAAAGATGCATCCTCAGATAAAAAAGGAGGGTCACAAGATGACAAGTCCCTGGTTGTTTGCTTTGGGGAAATGCTAATTGACTTTGTGCCCACCGTTGGTGGAGTTTCACTTGCTGAAGCACCTGCTTTTAATAAAGCTCCTGGTGGTGCTCCTGCTAATGTCGCTGTTGGAGTCGCAAGACTGGGTGGTTCAGCAGGTTTCATAGGCAAGGTAGGTGATGATGAATTTGGATACATGTTGGCTGACATTTTAAAACAAAACAATGTTGACAATTCTGGCGTGCGATTTGACCCCAGTGCAAGAACTGCATTGGCATTTGTTACACTCAGAGCTGATGGAGAGCGTGAATTCCTTTTTTTCCGTCATCCAAGTGCTGATATGCTTCTACGTGAATCAGAGCTTGACATAAATCTTATTAAGAAGGCAAAAATTTTCCATTACGGTTCAATTAGTTTAATTGATGAACCATGCAGGTCAACCCATCTTGCTGCAATGAAAATTGCCAAGAAGTCTGGTTGCATCCTTTCTTATGATCCAAATTTAAGATTGCCATTGTGGCCATCAGAAGATGCCGCACTGAAAGGCATAATGAGTATATGGGATCAAGCAGATATTATCAAGATAAGTGAGGATGAGATTAGATTCCTGACTGGTGGTGATGATCCTTGCGATGATAATGTGGTGTTAACGAAGCTTTTTCATCCTAATCTTAGACTTTTGGTAGTAACTGAAGGATCAGAGGGTTGCAGATATTACACACAGAAATTCCATGGGCGGGTTGGTGGTGTTAAAGTTAAACCTGTTGACACAACTGGTGCTGGTGATGCATTCGTTGGTGGGGTACTGAACAGCATAGCTTCTAACTTGAGTCTTTTTCAGGACGAGAAGGGGTTACGAGAAGCTCTACTCTTTGCGAATGCATGCGGTGCGCTCACAGTAACAGAAAGAGGAGCCATTCCTGCAATGCCGACAAGAGAAGCCGTGCTCCAGTGCTTAAAGCAGGCTGCTGAGAAAAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004396 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005996 GO:0006000 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008610 GO:0008865 GO:0009058 GO:0009987 GO:0010035 GO:0010038 GO:0016051 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019200 GO:0019318 GO:0019752 GO:0032787 GO:0042221 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044262 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046686 GO:0046835 GO:0050896 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00051 ko00500 ko00520 ko01100 map00051 map00500 map00520 map01100

Pfam Domains

Protein Families

PfkB

Protein Analysis

359

Amino Acids

38.38

Weight (kDa)

5.53

Isoelectric Point (pI)

32.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PfkB	PF00294	35 - 343	6.4e-77	pfkB family carbohydrate kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003570)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51830 AT5G51830
fragaria_vesca	FvH4_7g12690 FvH4_7g28710 FvH4_7g28710
malus_domestica	MD00G1004100.v1.1 MD01G1193000.v1.1 MD02G1197500.v1.1 MD15G1307900.v1.1
prunus_persica	Prupe.2G151100_v2.0.a1 Prupe.2G287100_v2.0.a1 Prupe.2G287100_v2.0.a1 Prupe.2G287100_v2.0.a1
pyrus_communis	pycom01g20360 pycom02g16100 pycom07g23930
rosa_chinensis	RchiOBHm_Chr1g0351681 RchiOBHm_Chr1g0376211
rosa_laevigata	RLG00000026619 RLG00000028390
rosa_multiflora	Rmu_sc0000061.1_g000037 Rmu_sc0001144.1_g000004
rosa_roxburghii	Rroxscaffold_4G00282300 Rroxscaffold_4G00303620
rosa_rugosa	Rorug01G0220600 Rorug01G0394400
rosa_samantha	Rh1AG406000 Rh1BG369700 Rh1CG382400 Rh1CG382600 Rh1DG232200 Rh1DG399300 Rh1DG399500
rosa_wichuraiana	Rw1G020430 Rw1G036020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	187
Acc36I	ACCTGC	3 cut(s)	187, 242, 519
AciI	CCGC	3 cut(s)	630, 832, 981
AclWI	GGATC	4 cut(s)	584, 666, 713, 799
AcoI	YGGCCR	1 cut(s)	614
AcsI	RAATTY	5 cut(s)	281, 404, 486, 595, 821
AcuI	CTGAAG	2 cut(s)	192, 807
AfaI	GTAC	1 cut(s)	900
AfiI	CCNNNNNNNGG	3 cut(s)	25, 152, 832
AflIII	ACRYGT	1 cut(s)	291
AgsI	TTSAA	1 cut(s)	504
AjnI	CCWGG	2 cut(s)	105, 196
AluBI	AGCT	7 cut(s)	23, 194, 389, 457, 751, 914, 959
AluI	AGCT	7 cut(s)	23, 194, 389, 457, 751, 914, 959
Alw21I	GWGCWC	2 cut(s)	208, 1044
AlwI	GGATC	4 cut(s)	584, 666, 713, 799
AoxI	GGCC	2 cut(s)	16, 614
ApeKI	GCWGC	2 cut(s)	545, 1061
ApoI	RAATTY	5 cut(s)	281, 404, 486, 595, 821
AspLEI	GCGC	1 cut(s)	988
AspS9I	GGNCC	1 cut(s)	17
AsuHPI	GGTGA	3 cut(s)	284, 725, 890
BaeGI	GKGCMC	1 cut(s)	147
BalI	TGGCCA	1 cut(s)	616
Bbv12I	GWGCWC	2 cut(s)	208, 1044
BbvI	GCAGC	2 cut(s)	532, 1048
BccI	CCATC	3 cut(s)	386, 546, 625
BceAI	ACGGC	1 cut(s)	1022
BciT130I	CCWGG	2 cut(s)	107, 198
BciVI	GTATCC	1 cut(s)	281
BfuAI	ACCTGC	3 cut(s)	187, 242, 519
BfuI	GTATCC	1 cut(s)	281
BglI	GCCNNNNNGGC	1 cut(s)	613
BisI	GCNGC	3 cut(s)	546, 630, 1062
BlsI	GCNGC	3 cut(s)	547, 631, 1063
Bme1390I	CCNGG	2 cut(s)	107, 198
BmgT120I	GGNCC	1 cut(s)	17
BmiI	GGNNCC	1 cut(s)	1009
BmrFI	CCNGG	2 cut(s)	107, 198
BmrI	ACTGGG	2 cut(s)	248, 345
BmsI	GCATC	5 cut(s)	52, 74, 585, 616, 871
BmuI	ACTGGG	2 cut(s)	248, 345
BpmI	CTGGAG	1 cut(s)	1028
BpuEI	CTTGAG	1 cut(s)	943
BsaAI	YACGTR	1 cut(s)	446
BsaJI	CCNNGG	2 cut(s)	105, 826
Bsc4I	CCNNNNNNNGG	3 cut(s)	25, 152, 832
Bse1I	ACTGG	5 cut(s)	243, 351, 712, 874, 1045
Bse3DI	GCAATG	2 cut(s)	555, 1026
BseBI	CCWGG	2 cut(s)	107, 198
BseDI	CCNNGG	2 cut(s)	105, 826
BseGI	GGATG	5 cut(s)	65, 421, 576, 694, 757
BseLI	CCNNNNNNNGG	3 cut(s)	25, 152, 832
BseMI	GCAATG	2 cut(s)	555, 1026
BseMII	CTCAG	3 cut(s)	84, 397, 1056
BseNI	ACTGG	5 cut(s)	243, 351, 712, 874, 1045
BseRI	GAGGAG	1 cut(s)	1020
BseSI	GKGCMC	1 cut(s)	147
BseXI	GCAGC	2 cut(s)	532, 1048
BseYI	CCCAGC	1 cut(s)	19
BshFI	GGCC	2 cut(s)	18, 616
BsiHKAI	GWGCWC	2 cut(s)	208, 1044
BslFI	GGGAC	1 cut(s)	88
BslI	CCNNNNNNNGG	3 cut(s)	25, 152, 832
BsmFI	GGGAC	1 cut(s)	88
BsmI	GAATGC	2 cut(s)	884, 979
BsnI	GGCC	2 cut(s)	18, 616
Bsp1286I	GDGCHC	3 cut(s)	147, 208, 1044
Bsp143I	GATC	4 cut(s)	589, 658, 718, 791
Bsp19I	CCATGG	1 cut(s)	826
BspACI	CCGC	3 cut(s)	630, 832, 981
BspANI	GGCC	2 cut(s)	18, 616
BspCNI	CTCAG	3 cut(s)	83, 396, 1057
BspLI	GGNNCC	1 cut(s)	1009
BspMI	ACCTGC	3 cut(s)	187, 242, 519
BspPI	GGATC	4 cut(s)	584, 666, 713, 799
BsrDI	GCAATG	2 cut(s)	555, 1026
BsrI	ACTGG	5 cut(s)	243, 351, 712, 874, 1045
BssECI	CCNNGG	2 cut(s)	105, 826
BssMI	GATC	4 cut(s)	589, 658, 718, 791
BssT1I	CCWWGG	1 cut(s)	826
Bst2UI	CCWGG	2 cut(s)	107, 198
Bst4CI	ACNGT	3 cut(s)	151, 500, 994
BstAPI	GCANNNNNTGC	2 cut(s)	362, 983
BstBAI	YACGTR	1 cut(s)	446
BstC8I	GCNNGC	3 cut(s)	338, 979, 1059
BstDEI	CTNAG	4 cut(s)	70, 383, 767, 1065
BstDSI	CCRYGG	1 cut(s)	826
BstF5I	GGATG	5 cut(s)	65, 421, 576, 694, 757
BstHHI	GCGC	1 cut(s)	988
BstKTI	GATC	4 cut(s)	592, 661, 721, 794
BstMBI	GATC	4 cut(s)	589, 658, 718, 791
BstMWI	GCNNNNNNNGC	6 cut(s)	218, 362, 613, 881, 983, 1016
BstNI	CCWGG	2 cut(s)	107, 198
BstNSI	RCATGY	2 cut(s)	295, 981
BstSCI	CCNGG	2 cut(s)	105, 196
BstSLI	GKGCMC	1 cut(s)	147
BstV1I	GCAGC	2 cut(s)	532, 1048
BsuI	GTATCC	1 cut(s)	281
BsuRI	GGCC	2 cut(s)	18, 616
BtgI	CCRYGG	1 cut(s)	826
BtgZI	GCGATG	1 cut(s)	741
BtsCI	GGATG	5 cut(s)	65, 421, 576, 694, 757
BtsIMutI	CAGTG	3 cut(s)	358, 632, 1052
BveI	ACCTGC	3 cut(s)	187, 242, 519
Cac8I	GCNNGC	3 cut(s)	338, 979, 1059
CfoI	GCGC	1 cut(s)	988
Cfr13I	GGNCC	1 cut(s)	17
Csp6I	GTAC	1 cut(s)	899
CviAII	CATG	4 cut(s)	292, 525, 827, 978
CviQI	GTAC	1 cut(s)	899
DdeI	CTNAG	4 cut(s)	70, 383, 767, 1065
DpnI	GATC	4 cut(s)	591, 660, 720, 793
DpnII	GATC	4 cut(s)	589, 658, 718, 791
DraI	TTTAAA	1 cut(s)	309
EaeI	YGGCCR	1 cut(s)	614
Eco130I	CCWWGG	1 cut(s)	826
Eco57I	CTGAAG	2 cut(s)	192, 807
EcoRI	GAATTC	1 cut(s)	404
EcoRII	CCWGG	2 cut(s)	105, 196
EcoT14I	CCWWGG	1 cut(s)	826
EcoT22I	ATGCAT	3 cut(s)	67, 886, 979
ErhI	CCWWGG	1 cut(s)	826
FaeI	CATG	4 cut(s)	295, 528, 830, 981
FaqI	GGGAC	1 cut(s)	88
FatI	CATG	4 cut(s)	291, 524, 826, 977
FauI	CCCGC	1 cut(s)	825
Fnu4HI	GCNGC	3 cut(s)	546, 630, 1062
FokI	GGATG	5 cut(s)	52, 408, 563, 701, 744
Fsp4HI	GCNGC	3 cut(s)	546, 630, 1062
GlaI	GCGC	1 cut(s)	987
GluI	GCNGC	3 cut(s)	546, 630, 1062
GsaI	CCCAGC	1 cut(s)	23
GsuI	CTGGAG	1 cut(s)	1028
HaeIII	GGCC	2 cut(s)	18, 616
HhaI	GCGC	1 cut(s)	988
Hin1II	CATG	4 cut(s)	295, 528, 830, 981
Hin6I	GCGC	1 cut(s)	986
HinP1I	GCGC	1 cut(s)	986
HincII	GTYRAC	4 cut(s)	325, 534, 744, 862
HindII	GTYRAC	4 cut(s)	325, 534, 744, 862
HindIII	AAGCTT	1 cut(s)	749
HinfI	GANTC	5 cut(s)	53, 228, 449, 699, 925
HpaI	GTTAAC	1 cut(s)	744
HphI	GGTGA	3 cut(s)	284, 725, 890
Hpy166II	GTNNAC	4 cut(s)	325, 534, 744, 862
Hpy188I	TCNGA	5 cut(s)	73, 386, 454, 622, 796
Hpy188III	TCNNGA	4 cut(s)	26, 676, 703, 935
Hpy8I	GTNNAC	4 cut(s)	325, 534, 744, 862
HpyAV	CCTTC	3 cut(s)	472, 782, 937
HpyCH4III	ACNGT	3 cut(s)	151, 500, 994
HpyCH4IV	ACGT	1 cut(s)	445
HpyF10VI	GCNNNNNNNGC	6 cut(s)	218, 362, 613, 881, 983, 1016
HpyF3I	CTNAG	4 cut(s)	70, 383, 767, 1065
HpySE526I	ACGT	1 cut(s)	445
Hsp92II	CATG	4 cut(s)	295, 528, 830, 981
HspAI	GCGC	1 cut(s)	986
KspAI	GTTAAC	1 cut(s)	744
Kzo9I	GATC	4 cut(s)	589, 658, 718, 791
LmnI	GCTCC	4 cut(s)	199, 211, 1007, 1047
Lsp1109I	GCAGC	2 cut(s)	532, 1048
LweI	GCATC	5 cut(s)	52, 74, 585, 616, 871
MaeII	ACGT	1 cut(s)	445
MaeIII	GTNAC	5 cut(s)	87, 376, 781, 948, 994
MalI	GATC	4 cut(s)	591, 660, 720, 793
MboI	GATC	4 cut(s)	589, 658, 718, 791
MboII	GAAGA	2 cut(s)	62, 635
MhlI	GDGCHC	3 cut(s)	147, 208, 1044
MlsI	TGGCCA	1 cut(s)	616
MluNI	TGGCCA	1 cut(s)	616
MlyI	GAGTC	2 cut(s)	237, 934
MmeI	TCCRAC	1 cut(s)	205
MnlI	CCTC	5 cut(s)	77, 79, 679, 790, 998
Mox20I	TGGCCA	1 cut(s)	616
Mph1103I	ATGCAT	3 cut(s)	67, 886, 979
MscI	TGGCCA	1 cut(s)	616
MseI	TTAA	9 cut(s)	186, 308, 474, 512, 599, 743, 846, 852, 1052
MslI	CAYNNNNRTG	1 cut(s)	426
Msp20I	TGGCCA	1 cut(s)	616
MspR9I	CCNGG	2 cut(s)	107, 198
Mva1269I	GAATGC	2 cut(s)	884, 979
MvaI	CCWGG	2 cut(s)	107, 198
MwoI	GCNNNNNNNGC	6 cut(s)	218, 362, 613, 881, 983, 1016
NcoI	CCATGG	1 cut(s)	826
NdeII	GATC	4 cut(s)	589, 658, 718, 791
NlaIII	CATG	4 cut(s)	295, 528, 830, 981
NlaIV	GGNNCC	1 cut(s)	1009
NmuCI	GTSAC	1 cut(s)	87
NsiI	ATGCAT	3 cut(s)	67, 886, 979
NspI	RCATGY	2 cut(s)	295, 981
PaeI	GCATGC	1 cut(s)	981
PaqCI	CACCTGC	1 cut(s)	187
PciI	ACATGT	1 cut(s)	291
PctI	GAATGC	2 cut(s)	884, 979
PfeI	GAWTC	3 cut(s)	53, 449, 699
PkrI	GCNGC	3 cut(s)	547, 631, 1063
PleI	GAGTC	2 cut(s)	236, 933
PpsI	GAGTC	2 cut(s)	236, 933
Ppu21I	YACGTR	1 cut(s)	446
PscI	ACATGT	1 cut(s)	291
Psp6I	CCWGG	2 cut(s)	105, 196
PspFI	CCCAGC	1 cut(s)	19
PspGI	CCWGG	2 cut(s)	105, 196
PspN4I	GGNNCC	1 cut(s)	1009
PspPI	GGNCC	1 cut(s)	17
RsaI	GTAC	1 cut(s)	900
RsaNI	GTAC	1 cut(s)	899
RseI	CAYNNNNRTG	1 cut(s)	426
SaqAI	TTAA	9 cut(s)	186, 308, 474, 512, 599, 743, 846, 852, 1052
SatI	GCNGC	3 cut(s)	546, 630, 1062
Sau3AI	GATC	4 cut(s)	589, 658, 718, 791
Sau96I	GGNCC	1 cut(s)	17
SchI	GAGTC	2 cut(s)	237, 934
ScrFI	CCNGG	2 cut(s)	107, 198
SduI	GDGCHC	3 cut(s)	147, 208, 1044
SfaNI	GCATC	5 cut(s)	52, 74, 585, 616, 871
SmiMI	CAYNNNNRTG	1 cut(s)	426
SmlI	CTYRAG	1 cut(s)	922
SmoI	CTYRAG	1 cut(s)	922
SphI	GCATGC	1 cut(s)	981
SsiI	CCGC	3 cut(s)	630, 832, 981
StyD4I	CCNGG	2 cut(s)	105, 196
StyI	CCWWGG	1 cut(s)	826
TaaI	ACNGT	3 cut(s)	151, 500, 994
TaiI	ACGT	1 cut(s)	448
TauI	GCSGC	1 cut(s)	632
TfiI	GAWTC	3 cut(s)	53, 449, 699
Tru1I	TTAA	9 cut(s)	186, 308, 474, 512, 599, 743, 846, 852, 1052
Tru9I	TTAA	9 cut(s)	186, 308, 474, 512, 599, 743, 846, 852, 1052
TscAI	CASTG	3 cut(s)	358, 639, 1052
TseFI	GTSAC	1 cut(s)	87
TseI	GCWGC	2 cut(s)	545, 1061
Tsp45I	GTSAC	1 cut(s)	87
TspDTI	ATGAA	5 cut(s)	247, 294, 534, 566, 746
TspGWI	ACGGA	1 cut(s)	407
TspRI	CASTG	3 cut(s)	358, 639, 1052
XapI	RAATTY	5 cut(s)	281, 404, 486, 595, 821
XceI	RCATGY	2 cut(s)	295, 981
XcmI	CCANNNNNNNNNTGG	1 cut(s)	833
Zsp2I	ATGCAT	3 cut(s)	67, 886, 979

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.