Rroxscaffold_4G00303620

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003570
Physical Location & Seq
Reverse (-)
23995509 .. 23998785
3277 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00303620.1

Sequence Viewer

Length: 1101 bp
ATGCCGATGGGTTGTGGCTGCTTCCCCAGGACATCCCGAAAGTCCAATCACCTCAAGTTACCGAGGCTGCCCTCCATGACCGATTCAATCAAATCATTATCTCCCAGGAAAAAGAATGCCCCAGAGAGGAGCACACTGGTCGTTTGCTTCGGAGAGATGTTGATTGATTTTGTCCCAACGAGTGCTGGAGTTTCTCTTGCTGATGCAGAAGGTTTCAAGAAAGCTGCTGGTGGAGCTCCTGCTAATGTTGCAGTTGGAATCTCAAGATTAGGAGGCTCAGCAGCTTTTATGGGCAAGGTTGGTGCAGATGAGTTTGGATACATGCTGTCTAATATTCTGAAAGAAAACAATGTGGACAATTCCGGCATGTGTTTTGACCAACATGCAAGGACTGCATTGGCTTTTGTTACACTGAGAAGTGATGGAGAACGCGAATTCATGTTCTATCGCAACCCGAGTGCTGATATGCTTCTCACTAAGGAAGAACTTAACGTCAACCTAATCAAAAAGGCAAATATATTTCACTATGGGTCGATTAGTCTGATTGAGGAACCATGTAAATCAGCACATCTCGCTGCGATGGACGTTGCTAAGAAGGCTGGCTGTATCCTCTCTTATGACCCGAACTTGAGATTGCCACTGTGGCCTTCTGCAGAGGCTGCTCAGAAGGGCATTATGAGCATTTGGAACCTAGCTGACATTATTAAAATAAGTGAGGAGGAAATTACATTTTTGACTGGAGGTGATGATCCTTATGATGATGATGTGGTCTTGAACAAGCTTTATCACCCCAATCTGAAGCTTCTGTTAGTAACTGAAGGGCCAGCAGGCTGCAGATACTACACAAAGGAATTCAAGGGTAAAGTTGCAGGAATCAAAGCGGACTGTGTAGATACAACTGGAGCTGGTGACTCTTTTGTTGGCTCTGTACTCACCAGCTTGGCTTCAGACCCTGATTTGTTCAAGAATGAACAACGGTTGAAGGAGGCTCTCAAATTTGCCAATGCTTGTGGTGCTATCACTGTGGAAAAGAAAGGAGCCATTCCTGCCCTACCCACAAAAGCAGCTGTCCATAAAGTTCTTAGCCAAACATCGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00500 ko00520 ko01100 map00051 map00500 map00520 map01100
Pfam Domains
Protein Families
PfkB

Protein Analysis

366

Amino Acids

39.38

Weight (kDa)

7.99

Isoelectric Point (pI)

33.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 45 - 353 5.5e-76 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 432
AciI CCGC 1 cut(s) 881
AclWI GGATC 1 cut(s) 743
AcsI RAATTY 3 cut(s) 434, 851, 995
AcuI CTGAAG 3 cut(s) 818, 837, 930
AfaI GTAC 1 cut(s) 930
AfiI CCNNNNNNNGG 2 cut(s) 126, 1093
AgsI TTSAA 6 cut(s) 87, 217, 775, 856, 964, 982
AjnI CCWGG 2 cut(s) 26, 104
AluBI AGCT 9 cut(s) 224, 236, 284, 695, 781, 802, 905, 939, 1067
AluI AGCT 9 cut(s) 224, 236, 284, 695, 781, 802, 905, 939, 1067
Alw21I GWGCWC 2 cut(s) 134, 238
AlwI GGATC 1 cut(s) 743
AlwNI CAGNNNCTG 2 cut(s) 659, 953
Ama87I CYCGRG 1 cut(s) 454
AoxI GGCC 2 cut(s) 644, 821
ApeKI GCWGC 8 cut(s) 18, 67, 224, 281, 575, 659, 831, 1064
ApoI RAATTY 3 cut(s) 434, 851, 995
AspS9I GGNCC 1 cut(s) 821
AsuHPI GGTGA 5 cut(s) 41, 755, 779, 920, 925
AvaI CYCGRG 1 cut(s) 454
BanII GRGCYC 1 cut(s) 238
Bbv12I GWGCWC 2 cut(s) 134, 238
BbvI GCAGC 8 cut(s) 5, 54, 211, 293, 562, 646, 818, 1076
BccI CCATC 2 cut(s) 416, 574
BcgI CGANNNNNNTGC 2 cut(s) 121, 155
BciT130I CCWGG 2 cut(s) 28, 106
BciVI GTATCC 2 cut(s) 311, 617
BfaI CTAG 1 cut(s) 692
BfmI CTRYAG 2 cut(s) 651, 832
BfuI GTATCC 2 cut(s) 311, 617
BglI GCCNNNNNGGC 1 cut(s) 643
BisI GCNGC 8 cut(s) 19, 68, 225, 282, 576, 660, 832, 1065
BlpI GCTNAGC 1 cut(s) 277
BlsI GCNGC 8 cut(s) 20, 69, 226, 283, 577, 661, 833, 1066
Bme1390I CCNGG 2 cut(s) 28, 106
BmeT110I CYCGRG 1 cut(s) 454
BmgT120I GGNCC 1 cut(s) 821
BmiI GGNNCC 3 cut(s) 552, 689, 1039
BmrFI CCNGG 2 cut(s) 28, 106
BmsI GCATC 1 cut(s) 193
BpmI CTGGAG 3 cut(s) 207, 759, 921
Bpu1102I GCTNAGC 1 cut(s) 277
BpuEI CTTGAG 3 cut(s) 38, 247, 649
BsaJI CCNNGG 3 cut(s) 26, 62, 104
Bsc4I CCNNNNNNNGG 2 cut(s) 126, 1093
Bse1I ACTGG 3 cut(s) 141, 742, 904
BseBI CCWGG 2 cut(s) 28, 106
BseDI CCNNGG 3 cut(s) 26, 62, 104
BseGI GGATG 1 cut(s) 32
BseLI CCNNNNNNNGG 2 cut(s) 126, 1093
BseMII CTCAG 3 cut(s) 291, 404, 677
BseNI ACTGG 3 cut(s) 141, 742, 904
BseRI GAGGAG 2 cut(s) 142, 731
BseXI GCAGC 8 cut(s) 5, 54, 211, 293, 562, 646, 818, 1076
BsgI GTGCAG 1 cut(s) 324
Bsh1236I CGCG 1 cut(s) 432
BshFI GGCC 2 cut(s) 646, 823
BsiHKAI GWGCWC 2 cut(s) 134, 238
BsiHKCI CYCGRG 1 cut(s) 454
BsiSI CCGG 1 cut(s) 363
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 2 cut(s) 126, 1093
BsmFI GGGAC 1 cut(s) 158
BsmI GAATGC 1 cut(s) 121
BsnI GGCC 2 cut(s) 646, 823
BsoBI CYCGRG 1 cut(s) 454
Bsp1286I GDGCHC 2 cut(s) 134, 238
Bsp143I GATC 1 cut(s) 748
Bsp1720I GCTNAGC 1 cut(s) 277
BspACI CCGC 1 cut(s) 881
BspANI GGCC 2 cut(s) 646, 823
BspCNI CTCAG 3 cut(s) 290, 405, 676
BspFNI CGCG 1 cut(s) 432
BspLI GGNNCC 3 cut(s) 552, 689, 1039
BspMAI CTGCAG 2 cut(s) 655, 836
BspPI GGATC 1 cut(s) 743
BsrI ACTGG 3 cut(s) 141, 742, 904
BssECI CCNNGG 3 cut(s) 26, 62, 104
BssMI GATC 1 cut(s) 748
Bst2UI CCWGG 2 cut(s) 28, 106
Bst4CI ACNGT 4 cut(s) 642, 887, 978, 1024
BstAPI GCANNNNNTGC 2 cut(s) 392, 659
BstC8I GCNNGC 3 cut(s) 601, 825, 829
BstDEI CTNAG 6 cut(s) 277, 413, 477, 591, 663, 1082
BstF5I GGATG 1 cut(s) 32
BstFNI CGCG 1 cut(s) 432
BstKTI GATC 1 cut(s) 751
BstMBI GATC 1 cut(s) 748
BstNI CCWGG 2 cut(s) 28, 106
BstNSI RCATGY 3 cut(s) 325, 370, 386
BstSCI CCNGG 2 cut(s) 26, 104
BstSFI CTRYAG 2 cut(s) 651, 832
BstUI CGCG 1 cut(s) 432
BstV1I GCAGC 8 cut(s) 5, 54, 211, 293, 562, 646, 818, 1076
BsuI GTATCC 2 cut(s) 311, 617
BsuRI GGCC 2 cut(s) 646, 823
BtgZI GCGATG 1 cut(s) 593
BtsCI GGATG 1 cut(s) 32
BtsIMutI CAGTG 4 cut(s) 134, 410, 638, 1020
Cac8I GCNNGC 3 cut(s) 601, 825, 829
CaiI CAGNNNCTG 2 cut(s) 659, 953
Cfr13I GGNCC 1 cut(s) 821
Csp6I GTAC 1 cut(s) 929
CspCI CAANNNNNGTGG 2 cut(s) 991, 1026
CviAII CATG 7 cut(s) 76, 322, 367, 383, 439, 555, 1098
CviQI GTAC 1 cut(s) 929
DdeI CTNAG 6 cut(s) 277, 413, 477, 591, 663, 1082
DpnI GATC 1 cut(s) 750
DpnII GATC 1 cut(s) 748
Ecl136II GAGCTC 1 cut(s) 236
Eco24I GRGCYC 1 cut(s) 238
Eco53kI GAGCTC 1 cut(s) 236
Eco57I CTGAAG 3 cut(s) 818, 837, 930
Eco88I CYCGRG 1 cut(s) 454
EcoICRI GAGCTC 1 cut(s) 236
EcoRI GAATTC 2 cut(s) 434, 851
EcoRII CCWGG 2 cut(s) 26, 104
EcoT38I GRGCYC 1 cut(s) 238
FaeI CATG 7 cut(s) 79, 325, 370, 386, 442, 558, 1101
FaqI GGGAC 1 cut(s) 158
FatI CATG 7 cut(s) 75, 321, 366, 382, 438, 554, 1097
Fnu4HI GCNGC 8 cut(s) 19, 68, 225, 282, 576, 660, 832, 1065
FokI GGATG 1 cut(s) 19
FriOI GRGCYC 1 cut(s) 238
Fsp4HI GCNGC 8 cut(s) 19, 68, 225, 282, 576, 660, 832, 1065
FspBI CTAG 1 cut(s) 692
GluI GCNGC 8 cut(s) 19, 68, 225, 282, 576, 660, 832, 1065
GsuI CTGGAG 3 cut(s) 207, 759, 921
HaeIII GGCC 2 cut(s) 646, 823
HapII CCGG 1 cut(s) 363
Hin1II CATG 7 cut(s) 79, 325, 370, 386, 442, 558, 1101
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HindIII AAGCTT 2 cut(s) 779, 800
HinfI GANTC 4 cut(s) 83, 258, 873, 911
HpaII CCGG 1 cut(s) 363
HphI GGTGA 5 cut(s) 41, 755, 779, 920, 925
Hpy166II GTNNAC 2 cut(s) 355, 496
Hpy188I TCNGA 6 cut(s) 152, 339, 543, 666, 798, 949
Hpy188III TCNNGA 5 cut(s) 36, 217, 264, 772, 964
Hpy8I GTNNAC 2 cut(s) 355, 496
HpyAV CCTTC 6 cut(s) 203, 589, 657, 661, 812, 976
HpyCH4III ACNGT 4 cut(s) 642, 887, 978, 1024
HpyCH4IV ACGT 2 cut(s) 492, 585
HpyCH4V TGCA 8 cut(s) 206, 251, 305, 386, 395, 653, 834, 869
HpyF3I CTNAG 6 cut(s) 277, 413, 477, 591, 663, 1082
HpySE526I ACGT 2 cut(s) 492, 585
Hsp92II CATG 7 cut(s) 79, 325, 370, 386, 442, 558, 1101
Kzo9I GATC 1 cut(s) 748
LmnI GCTCC 5 cut(s) 129, 233, 241, 902, 1037
Lsp1109I GCAGC 8 cut(s) 5, 54, 211, 293, 562, 646, 818, 1076
LweI GCATC 1 cut(s) 193
MaeI CTAG 1 cut(s) 692
MaeII ACGT 2 cut(s) 492, 585
MaeIII GTNAC 4 cut(s) 57, 406, 811, 908
MalI GATC 1 cut(s) 750
MboI GATC 1 cut(s) 748
MboII GAAGA 1 cut(s) 494
MhlI GDGCHC 2 cut(s) 134, 238
MluCI AATT 5 cut(s) 358, 434, 723, 851, 995
MlyI GAGTC 1 cut(s) 905
MmeI TCCRAC 1 cut(s) 235
MseI TTAA 2 cut(s) 489, 705
MslI CAYNNNNRTG 1 cut(s) 1096
MspA1I CMGCKG 1 cut(s) 1067
MspI CCGG 1 cut(s) 363
MspR9I CCNGG 2 cut(s) 28, 106
Mva1269I GAATGC 1 cut(s) 121
MvaI CCWGG 2 cut(s) 28, 106
MvnI CGCG 1 cut(s) 432
NdeII GATC 1 cut(s) 748
NlaIII CATG 7 cut(s) 79, 325, 370, 386, 442, 558, 1101
NlaIV GGNNCC 3 cut(s) 552, 689, 1039
NmuCI GTSAC 1 cut(s) 908
NspI RCATGY 3 cut(s) 325, 370, 386
PctI GAATGC 1 cut(s) 121
PfeI GAWTC 3 cut(s) 83, 258, 873
PkrI GCNGC 8 cut(s) 20, 69, 226, 283, 577, 661, 833, 1066
PleI GAGTC 1 cut(s) 905
PpsI GAGTC 1 cut(s) 905
Psp124BI GAGCTC 1 cut(s) 238
Psp6I CCWGG 2 cut(s) 26, 104
PspGI CCWGG 2 cut(s) 26, 104
PspN4I GGNNCC 3 cut(s) 552, 689, 1039
PspPI GGNCC 1 cut(s) 821
PstI CTGCAG 2 cut(s) 655, 836
PstNI CAGNNNCTG 2 cut(s) 659, 953
PvuII CAGCTG 1 cut(s) 1067
RsaI GTAC 1 cut(s) 930
RsaNI GTAC 1 cut(s) 929
RseI CAYNNNNRTG 1 cut(s) 1096
SacI GAGCTC 1 cut(s) 238
SaqAI TTAA 2 cut(s) 489, 705
SatI GCNGC 8 cut(s) 19, 68, 225, 282, 576, 660, 832, 1065
Sau3AI GATC 1 cut(s) 748
Sau96I GGNCC 1 cut(s) 821
SchI GAGTC 1 cut(s) 905
ScrFI CCNGG 2 cut(s) 28, 106
SduI GDGCHC 2 cut(s) 134, 238
SfaNI GCATC 1 cut(s) 193
SfcI CTRYAG 2 cut(s) 651, 832
SmiMI CAYNNNNRTG 1 cut(s) 1096
SmlI CTYRAG 3 cut(s) 53, 262, 628
SmoI CTYRAG 3 cut(s) 53, 262, 628
Sse9I AATT 5 cut(s) 358, 434, 723, 851, 995
SsiI CCGC 1 cut(s) 881
SspI AATATT 1 cut(s) 334
SspMI CTAG 1 cut(s) 692
SstI GAGCTC 1 cut(s) 238
StyD4I CCNGG 2 cut(s) 26, 104
TaaI ACNGT 4 cut(s) 642, 887, 978, 1024
TaiI ACGT 2 cut(s) 495, 588
TaqI TCGA 1 cut(s) 533
TaqII GACCGA 1 cut(s) 95
TasI AATT 5 cut(s) 358, 434, 723, 851, 995
TatI WGTACW 1 cut(s) 928
TfiI GAWTC 3 cut(s) 83, 258, 873
Tru1I TTAA 2 cut(s) 489, 705
Tru9I TTAA 2 cut(s) 489, 705
TscAI CASTG 4 cut(s) 141, 417, 645, 1027
TseFI GTSAC 1 cut(s) 908
TseI GCWGC 8 cut(s) 18, 67, 224, 281, 575, 659, 831, 1064
Tsp45I GTSAC 1 cut(s) 908
TspDTI ATGAA 2 cut(s) 427, 984
TspRI CASTG 4 cut(s) 141, 417, 645, 1027
XapI RAATTY 3 cut(s) 434, 851, 995
XceI RCATGY 3 cut(s) 325, 370, 386
XspI CTAG 1 cut(s) 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.