Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0003570

Physical Location & Seq

Genomic Coordinates Forward (+)

29188609 .. 29192472

Gene Structure 3864 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1193000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1077 bp

ATGGCTAATAATCTCGCCCCAGCTCAGGACAAAAAATTGGTTTTGCGTGAAGATTCTAAAGATGAATCCTCAGATAAAAAAGGAGGGTCACAAGATGACAAGTCCCTGGTTGTTTGCTTCGGGGAAATGCTAATCGACTTTGTGCCCACAGTTAGTGGAGTTTCACTTGCTGAAGCATCTGCTTTTCAAAAAGCTCCTGGTGGTGCTCCTGCTAATGTCGCTGTTGGTGTTGCAAGACTGGGTGGTTCAGCAGGTTTCATAGGCAAGGTAGGCGATGATGAATTTGGATACATGTTGTCTGACATTCTAAAACAAAACAATGTTGACAATTCTGGTGTTCGATTTGACCCCAATGCAAGAACTGCATTGGCTTTTGTTACACTCAGAGCTGATGGAGAGCGTGAATTCCTGTTTTTCCGTCATCCAAGTGCTGATATGCTTCTACGTGAATCAGAGCTTGACATAAATCTAATTAAGAAGGCAAAGATCTTCCATTATGGTTCAATTAGTTTGATTGATGAACCATGCAGGTCAACCCATCTAGCTGCTATGAAAATTGCCAAGGAGTCTGGTTGCATCCTTTCTTATGATCCAAATTTAAGATTGCCGTTGTGGCCGTCGGAAGATGCTGCACGGAAAGGCATAATGAGTATATGGGATCAAGCAGATATAATCAAGATAAGCGAGGATGAAATTAGATTCCTGACTGGTGGTGATGATCCTTATGATGATAATGTGGTGTTAACGAAGCTATTTCATCCCAATCTTAGACTGTTGGTAGTAACTGAGGGTTCAGAGGGTTGCAGATATTACACTCAGAAATTTCACGGCCGGGTTGGTGGTGTTAAAGTTAAAGCTGTTGACACAACCGGTGCTGGTGATGCATTCGTCGGTGGGGTACTGGACAGCATAGCTTCTAACTTGAATCTTTTTCAGGATGAGAAGGGGCTACGAGAAGCTCTACTCTTCGCAAATGCATGCGGTGCGCTCACAGTAACAGAAAGAGGAGCCATTCCTGCAATGCCGACGAGAGAAGCTGTGCTCCAGTGCTTAAAGCAGGCTGCTGAGAAAAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004396 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005996 GO:0006000 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008610 GO:0008865 GO:0009058 GO:0009987 GO:0010035 GO:0010038 GO:0016051 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019200 GO:0019318 GO:0019752 GO:0032787 GO:0042221 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044262 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046686 GO:0046835 GO:0050896 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00051 ko00500 ko00520 ko01100 map00051 map00500 map00520 map01100

Pfam Domains

Protein Families

PfkB

Protein Analysis

359

Amino Acids

38.69

Weight (kDa)

5.23

Isoelectric Point (pI)

34.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PfkB	PF00294	35 - 343	3.1e-77	pfkB family carbohydrate kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003570)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51830 AT5G51830
fragaria_vesca	FvH4_7g12690 FvH4_7g28710 FvH4_7g28710
malus_domestica	MD00G1004100.v1.1 MD01G1193000.v1.1 MD02G1197500.v1.1 MD15G1307900.v1.1
prunus_persica	Prupe.2G151100_v2.0.a1 Prupe.2G287100_v2.0.a1 Prupe.2G287100_v2.0.a1 Prupe.2G287100_v2.0.a1
pyrus_communis	pycom01g20360 pycom02g16100 pycom07g23930
rosa_chinensis	RchiOBHm_Chr1g0351681 RchiOBHm_Chr1g0376211
rosa_laevigata	RLG00000026619 RLG00000028390
rosa_multiflora	Rmu_sc0000061.1_g000037 Rmu_sc0001144.1_g000004
rosa_roxburghii	Rroxscaffold_4G00282300 Rroxscaffold_4G00303620
rosa_rugosa	Rorug01G0220600 Rorug01G0394400
rosa_samantha	Rh1AG406000 Rh1BG369700 Rh1CG382400 Rh1CG382600 Rh1DG232200 Rh1DG399300 Rh1DG399500
rosa_wichuraiana	Rw1G020430 Rw1G036020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	242, 519
AciI	CCGC	1 cut(s)	981
AclWI	GGATC	3 cut(s)	584, 666, 713
AcoI	YGGCCR	2 cut(s)	614, 829
AcsI	RAATTY	4 cut(s)	281, 404, 595, 821
AcuI	CTGAAG	1 cut(s)	192
AfaI	GTAC	1 cut(s)	900
AfiI	CCNNNNNNNGG	1 cut(s)	25
AflIII	ACRYGT	1 cut(s)	291
AgeI	ACCGGT	1 cut(s)	869
AgsI	TTSAA	3 cut(s)	188, 504, 925
AjnI	CCWGG	2 cut(s)	105, 196
Alw21I	GWGCWC	2 cut(s)	208, 1044
AlwI	GGATC	3 cut(s)	584, 666, 713
AoxI	GGCC	2 cut(s)	614, 829
ApeKI	GCWGC	3 cut(s)	545, 629, 1061
ApoI	RAATTY	4 cut(s)	281, 404, 595, 821
AsiGI	ACCGGT	1 cut(s)	869
AspLEI	GCGC	1 cut(s)	988
AsuC2I	CCSGG	1 cut(s)	833
AsuHPI	GGTGA	2 cut(s)	725, 890
BaeGI	GKGCMC	1 cut(s)	147
Bbv12I	GWGCWC	2 cut(s)	208, 1044
BbvI	GCAGC	3 cut(s)	532, 616, 1048
BccI	CCATC	2 cut(s)	386, 546
BceAI	ACGGC	3 cut(s)	592, 601, 844
BciT130I	CCWGG	2 cut(s)	107, 198
BciVI	GTATCC	1 cut(s)	281
BcnI	CCSGG	1 cut(s)	833
BfaI	CTAG	2 cut(s)	542, 1075
BfuAI	ACCTGC	2 cut(s)	242, 519
BfuI	GTATCC	1 cut(s)	281
BglI	GCCNNNNNGGC	1 cut(s)	613
BglII	AGATCT	1 cut(s)	486
BisI	GCNGC	3 cut(s)	546, 630, 1062
BlsI	GCNGC	3 cut(s)	547, 631, 1063
Bme1390I	CCNGG	3 cut(s)	107, 198, 833
BmiI	GGNNCC	1 cut(s)	1009
BmrFI	CCNGG	3 cut(s)	107, 198, 833
BmrI	ACTGGG	1 cut(s)	248
BmsI	GCATC	4 cut(s)	185, 585, 616, 871
BmuI	ACTGGG	1 cut(s)	248
BpmI	CTGGAG	1 cut(s)	1028
Bpu10I	CCTNAGC	1 cut(s)	24
BpuMI	CCSGG	1 cut(s)	833
BsaAI	YACGTR	1 cut(s)	446
BsaJI	CCNNGG	2 cut(s)	105, 561
BsaWI	WCCGGW	1 cut(s)	869
Bsc4I	CCNNNNNNNGG	1 cut(s)	25
Bse118I	RCCGGY	1 cut(s)	869
Bse1I	ACTGG	4 cut(s)	243, 712, 906, 1045
Bse3DI	GCAATG	1 cut(s)	1026
BseBI	CCWGG	2 cut(s)	107, 198
BseDI	CCNNGG	2 cut(s)	105, 561
BseGI	GGATG	5 cut(s)	421, 576, 694, 757, 943
BseLI	CCNNNNNNNGG	1 cut(s)	25
BseMI	GCAATG	1 cut(s)	1026
BseMII	CTCAG	6 cut(s)	38, 84, 397, 777, 830, 1056
BseNI	ACTGG	4 cut(s)	243, 712, 906, 1045
BseRI	GAGGAG	1 cut(s)	1020
BseSI	GKGCMC	1 cut(s)	147
BseX3I	CGGCCG	1 cut(s)	829
BseXI	GCAGC	3 cut(s)	532, 616, 1048
BseYI	CCCAGC	1 cut(s)	19
BsgI	GTGCAG	1 cut(s)	615
Bsh1285I	CGRYCG	1 cut(s)	832
BshFI	GGCC	2 cut(s)	616, 831
BshTI	ACCGGT	1 cut(s)	869
BsiEI	CGRYCG	1 cut(s)	832
BsiHKAI	GWGCWC	2 cut(s)	208, 1044
BsiSI	CCGG	2 cut(s)	832, 870
BslFI	GGGAC	1 cut(s)	88
BslI	CCNNNNNNNGG	1 cut(s)	25
BsmFI	GGGAC	1 cut(s)	88
BsmI	GAATGC	1 cut(s)	884
BsnI	GGCC	2 cut(s)	616, 831
Bsp1286I	GDGCHC	3 cut(s)	147, 208, 1044
Bsp143I	GATC	4 cut(s)	486, 589, 658, 718
BspACI	CCGC	1 cut(s)	981
BspANI	GGCC	2 cut(s)	616, 831
BspCNI	CTCAG	6 cut(s)	37, 83, 396, 778, 829, 1057
BspLI	GGNNCC	1 cut(s)	1009
BspMI	ACCTGC	2 cut(s)	242, 519
BspPI	GGATC	3 cut(s)	584, 666, 713
BsrDI	GCAATG	1 cut(s)	1026
BsrFI	RCCGGY	1 cut(s)	869
BsrI	ACTGG	4 cut(s)	243, 712, 906, 1045
BssAI	RCCGGY	1 cut(s)	869
BssECI	CCNNGG	2 cut(s)	105, 561
BssMI	GATC	4 cut(s)	486, 589, 658, 718
BssT1I	CCWWGG	1 cut(s)	561
Bst2UI	CCWGG	2 cut(s)	107, 198
Bst4CI	ACNGT	3 cut(s)	151, 774, 994
Bst6I	CTCTTC	1 cut(s)	971
BstAPI	GCANNNNNTGC	2 cut(s)	362, 983
BstBAI	YACGTR	1 cut(s)	446
BstC8I	GCNNGC	2 cut(s)	979, 1059
BstDEI	CTNAG	7 cut(s)	24, 70, 383, 767, 786, 816, 1065
BstF5I	GGATG	5 cut(s)	421, 576, 694, 757, 943
BstHHI	GCGC	1 cut(s)	988
BstKTI	GATC	4 cut(s)	489, 592, 661, 721
BstMBI	GATC	4 cut(s)	486, 589, 658, 718
BstMCI	CGRYCG	1 cut(s)	832
BstMWI	GCNNNNNNNGC	7 cut(s)	218, 270, 362, 613, 881, 983, 1016
BstNI	CCWGG	2 cut(s)	107, 198
BstNSI	RCATGY	2 cut(s)	295, 981
BstSCI	CCNGG	3 cut(s)	105, 196, 831
BstSLI	GKGCMC	1 cut(s)	147
BstV1I	GCAGC	3 cut(s)	532, 616, 1048
BstX2I	RGATCY	1 cut(s)	486
BstYI	RGATCY	1 cut(s)	486
BstZI	CGGCCG	1 cut(s)	829
BsuI	GTATCC	1 cut(s)	281
BsuRI	GGCC	2 cut(s)	616, 831
BtgZI	GCGATG	1 cut(s)	288
BtsCI	GGATG	5 cut(s)	421, 576, 694, 757, 943
BtsIMutI	CAGTG	1 cut(s)	1052
BveI	ACCTGC	2 cut(s)	242, 519
Cac8I	GCNNGC	2 cut(s)	979, 1059
CfoI	GCGC	1 cut(s)	988
Cfr10I	RCCGGY	1 cut(s)	869
Csp6I	GTAC	1 cut(s)	899
CspAI	ACCGGT	1 cut(s)	869
CviAII	CATG	3 cut(s)	292, 525, 978
CviQI	GTAC	1 cut(s)	899
DdeI	CTNAG	7 cut(s)	24, 70, 383, 767, 786, 816, 1065
DpnI	GATC	4 cut(s)	488, 591, 660, 720
DpnII	GATC	4 cut(s)	486, 589, 658, 718
EaeI	YGGCCR	2 cut(s)	614, 829
EagI	CGGCCG	1 cut(s)	829
Eam1104I	CTCTTC	1 cut(s)	971
EarI	CTCTTC	1 cut(s)	971
EclXI	CGGCCG	1 cut(s)	829
Eco130I	CCWWGG	1 cut(s)	561
Eco52I	CGGCCG	1 cut(s)	829
Eco57I	CTGAAG	1 cut(s)	192
EcoRI	GAATTC	1 cut(s)	404
EcoRII	CCWGG	2 cut(s)	105, 196
EcoT14I	CCWWGG	1 cut(s)	561
EcoT22I	ATGCAT	2 cut(s)	886, 979
ErhI	CCWWGG	1 cut(s)	561
FaeI	CATG	3 cut(s)	295, 528, 981
FaqI	GGGAC	1 cut(s)	88
FatI	CATG	3 cut(s)	291, 524, 977
Fnu4HI	GCNGC	3 cut(s)	546, 630, 1062
FokI	GGATG	5 cut(s)	408, 563, 701, 744, 950
Fsp4HI	GCNGC	3 cut(s)	546, 630, 1062
FspBI	CTAG	2 cut(s)	542, 1075
GlaI	GCGC	1 cut(s)	987
GluI	GCNGC	3 cut(s)	546, 630, 1062
GsaI	CCCAGC	1 cut(s)	23
GsuI	CTGGAG	1 cut(s)	1028
HaeIII	GGCC	2 cut(s)	616, 831
HapII	CCGG	2 cut(s)	832, 870
HhaI	GCGC	1 cut(s)	988
Hin1II	CATG	3 cut(s)	295, 528, 981
Hin6I	GCGC	1 cut(s)	986
HinP1I	GCGC	1 cut(s)	986
HincII	GTYRAC	4 cut(s)	325, 534, 744, 862
HindII	GTYRAC	4 cut(s)	325, 534, 744, 862
HinfI	GANTC	6 cut(s)	53, 65, 449, 566, 699, 925
HpaI	GTTAAC	1 cut(s)	744
HpaII	CCGG	2 cut(s)	832, 870
HphI	GGTGA	2 cut(s)	725, 890
Hpy166II	GTNNAC	4 cut(s)	325, 534, 744, 862
Hpy188I	TCNGA	7 cut(s)	73, 301, 386, 454, 622, 796, 819
Hpy188III	TCNNGA	4 cut(s)	26, 676, 703, 935
Hpy8I	GTNNAC	4 cut(s)	325, 534, 744, 862
Hpy99I	CGWCG	3 cut(s)	622, 893, 1030
HpyAV	CCTTC	2 cut(s)	472, 937
HpyCH4III	ACNGT	3 cut(s)	151, 774, 994
HpyCH4IV	ACGT	1 cut(s)	445
HpyF10VI	GCNNNNNNNGC	7 cut(s)	218, 270, 362, 613, 881, 983, 1016
HpyF3I	CTNAG	7 cut(s)	24, 70, 383, 767, 786, 816, 1065
HpySE526I	ACGT	1 cut(s)	445
Hsp92II	CATG	3 cut(s)	295, 528, 981
HspAI	GCGC	1 cut(s)	986
KspAI	GTTAAC	1 cut(s)	744
Kzo9I	GATC	4 cut(s)	486, 589, 658, 718
LmnI	GCTCC	4 cut(s)	199, 211, 1007, 1047
Lsp1109I	GCAGC	3 cut(s)	532, 616, 1048
LweI	GCATC	4 cut(s)	185, 585, 616, 871
MaeI	CTAG	2 cut(s)	542, 1075
MaeII	ACGT	1 cut(s)	445
MaeIII	GTNAC	4 cut(s)	87, 376, 781, 994
MalI	GATC	4 cut(s)	488, 591, 660, 720
MboI	GATC	4 cut(s)	486, 589, 658, 718
MboII	GAAGA	4 cut(s)	62, 481, 635, 958
MflI	RGATCY	1 cut(s)	486
MhlI	GDGCHC	3 cut(s)	147, 208, 1044
MlyI	GAGTC	1 cut(s)	575
MmeI	TCCRAC	1 cut(s)	600
MnlI	CCTC	6 cut(s)	77, 79, 679, 781, 790, 998
Mph1103I	ATGCAT	2 cut(s)	886, 979
MseI	TTAA	6 cut(s)	474, 599, 743, 846, 852, 1052
MslI	CAYNNNNRTG	1 cut(s)	426
MspI	CCGG	2 cut(s)	832, 870
MspR9I	CCNGG	3 cut(s)	107, 198, 833
Mva1269I	GAATGC	1 cut(s)	884
MvaI	CCWGG	2 cut(s)	107, 198
MwoI	GCNNNNNNNGC	7 cut(s)	218, 270, 362, 613, 881, 983, 1016
NciI	CCSGG	1 cut(s)	833
NdeII	GATC	4 cut(s)	486, 589, 658, 718
NlaIII	CATG	3 cut(s)	295, 528, 981
NlaIV	GGNNCC	1 cut(s)	1009
NmuCI	GTSAC	1 cut(s)	87
NsiI	ATGCAT	2 cut(s)	886, 979
NspI	RCATGY	2 cut(s)	295, 981
PaeI	GCATGC	1 cut(s)	981
PciI	ACATGT	1 cut(s)	291
PctI	GAATGC	1 cut(s)	884
PfeI	GAWTC	5 cut(s)	53, 65, 449, 699, 925
PinAI	ACCGGT	1 cut(s)	869
PkrI	GCNGC	3 cut(s)	547, 631, 1063
PleI	GAGTC	1 cut(s)	574
PpsI	GAGTC	1 cut(s)	574
Ppu21I	YACGTR	1 cut(s)	446
PscI	ACATGT	1 cut(s)	291
Psp6I	CCWGG	2 cut(s)	105, 196
PspFI	CCCAGC	1 cut(s)	19
PspGI	CCWGG	2 cut(s)	105, 196
PspN4I	GGNNCC	1 cut(s)	1009
PsuI	RGATCY	1 cut(s)	486
RsaI	GTAC	1 cut(s)	900
RsaNI	GTAC	1 cut(s)	899
RseI	CAYNNNNRTG	1 cut(s)	426
SaqAI	TTAA	6 cut(s)	474, 599, 743, 846, 852, 1052
SatI	GCNGC	3 cut(s)	546, 630, 1062
Sau3AI	GATC	4 cut(s)	486, 589, 658, 718
SchI	GAGTC	1 cut(s)	575
ScrFI	CCNGG	3 cut(s)	107, 198, 833
SduI	GDGCHC	3 cut(s)	147, 208, 1044
SfaNI	GCATC	4 cut(s)	185, 585, 616, 871
SmiMI	CAYNNNNRTG	1 cut(s)	426
SphI	GCATGC	1 cut(s)	981
SsiI	CCGC	1 cut(s)	981
SspMI	CTAG	2 cut(s)	542, 1075
StyD4I	CCNGG	3 cut(s)	105, 196, 831
StyI	CCWWGG	1 cut(s)	561
TaaI	ACNGT	3 cut(s)	151, 774, 994
TaiI	ACGT	1 cut(s)	448
TaqI	TCGA	2 cut(s)	135, 340
TfiI	GAWTC	5 cut(s)	53, 65, 449, 699, 925
Tru1I	TTAA	6 cut(s)	474, 599, 743, 846, 852, 1052
Tru9I	TTAA	6 cut(s)	474, 599, 743, 846, 852, 1052
TscAI	CASTG	1 cut(s)	1052
TseFI	GTSAC	1 cut(s)	87
TseI	GCWGC	3 cut(s)	545, 629, 1061
Tsp45I	GTSAC	1 cut(s)	87
TspDTI	ATGAA	7 cut(s)	78, 247, 294, 534, 566, 705, 746
TspGWI	ACGGA	2 cut(s)	407, 649
TspRI	CASTG	1 cut(s)	1052
XapI	RAATTY	4 cut(s)	281, 404, 595, 821
XceI	RCATGY	2 cut(s)	295, 981
XspI	CTAG	2 cut(s)	542, 1075
Zsp2I	ATGCAT	2 cut(s)	886, 979

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.